Agricultural functional active protein preparation rich in selenium, calcium and zinc and preparation method thereof

Abstract

The invention belongs to the field of agriculture, and particularly relates to an agricultural functional active protein preparation rich in selenium, calcium and zinc and a preparation method thereof. The preparation method comprises the following steps: mixing and extracting traditional Chinese medicine raw materials, then preparing a traditional Chinese medicine activation culture solution, and performing post-fermentation. According to the agricultural functional active protein preparation rich in selenium, calcium and zinc, trace elements such as selenium, calcium and zinc are converted from a combined state or an inactive state into an active organic state, so that the preparation has no toxic effect on soil and environment; after being biologically converted into an active organic state, the leaf spray has high absorption efficiency on agricultural products, and the leaf spray absorption efficiency can reach 90% or above; foliage spraying can be carried out, use is convenient, the labor cost is low when the fertilizer is applied to each mu of land, and multiple trace elements such as selenium, calcium and zinc are supplemented at the same time; organic selenium which is not easily absorbed by crops can be converted into active organic selenium which is easily absorbed by plants, efficient absorption and conversion of selenium are promoted, various pesticide residues can be rapidly and effectively degraded, soil is restored, the crop yield is increased, and the quality of target organs is improved.

Description

Technical Field

[0001] The invention belongs to the field of agriculture, and specifically relates to a selenium-calcium-zinc-rich agricultural functional active protein preparation and a preparation method thereof. Background Art

[0002] At present, chemical preparations such as sodium selenite are mostly used to supplement selenium in crops, but they have the following disadvantages:

[0003] (1) Selenium in its chemical form has a low absorption rate in plants, and in the form of sodium selenite it is toxic to the environment, crops, animals, and humans.

[0004] (2) The selenium content of the product is relatively low, and the amount absorbed and converted by plant organisms is even lower, which has a poor effect on increasing the selenium content in agricultural products.

[0005] (3) Failure to simultaneously supplement multiple trace elements such as selenium, calcium, and zinc.

[0006] (4) It fails to take into account the degradation of multiple pesticide residues and the restoration of soil.

[0007] (5) The effects of increasing crop yield and improving the quality of target organs are not significant.

[0008] Therefore, based on this, the technical solution of the present invention is proposed. Summary of the invention

[0009] In order to solve the problems existing in the prior art, the present invention provides a method for preparing a selenium-calcium-zinc-rich agricultural functional active protein preparation, the preparation method comprising the following steps:

[0010] (1) mixing liquorice and black tea, soaking the mixture in water, and then heating and extracting the mixture to obtain an extract;

[0011] (2) heating the extract and then sequentially adding peptone, yeast extract, white sugar, and sodium chloride, and mixing them evenly to obtain a traditional Chinese medicine activation culture solution;

[0012] (3) adding red wine koji (purchased from Shiyan Gufeng Xinyu Food Co., Ltd.) to the activated Chinese medicine culture solution, and fermenting under aerobic conditions to obtain a first fermentation solution;

[0013] (4) adding calcium chloride and zinc sulfate heptahydrate to the first fermentation liquid in sequence, and continuing the fermentation under aerobic conditions to obtain a second fermentation liquid;

[0014] (5) adding photosynthetic bacteria (purchased from Xiamen Mingde Weiye Biotechnology Co., Ltd.), actinomycetes (purchased from Beijing Kezhan Biotechnology Co., Ltd.), and Bacillus subtilis (purchased from Beijing Kezhan Biotechnology Co., Ltd.) to the second fermentation liquid in sequence, and continuing fermentation under aerobic conditions to obtain a third fermentation liquid;

[0015] (6) adding organic selenium to the third fermentation liquid, and continuing the fermentation under aerobic conditions, to obtain the selenium-rich calcium-zinc agricultural functional active protein preparation.

[0016] Preferably, in step (1), the heating and leaching are performed three times; wherein:

[0017] The first extraction was carried out under boiling conditions for 4 hours;

[0018] The second extraction was carried out under boiling conditions for 2 h;

[0019] The third extraction was carried out under boiling conditions for 1 h.

[0020] Preferably, in step (1), the amounts of raw materials used are, by weight, 35-40 parts of licorice and 55-60 parts of black tea.

[0021] Preferably, in step (2), the amounts of raw materials used, by weight, are: 75-80 parts of peptone, 35-40 parts of yeast extract, 25-30 parts of white sugar, and 8-10 parts of sodium chloride.

[0022] Preferably, in step (3), the amount of raw materials used is, by weight: 6 to 8 parts of red wine koji.

[0023] Preferably, in step (4), the amounts of raw materials used, by weight, are: 25-30 parts of calcium chloride and 15-20 parts of zinc sulfate heptahydrate.

[0024] Preferably, in step (5), the amounts of raw materials used, by weight, are: 6-8 parts of photosynthetic bacteria, 8-10 parts of actinomycetes, and 13-15 parts of Bacillus subtilis.

[0025] Preferably, in step (6), the amount of raw materials used, by weight, is: 3 parts of organic selenium; the organic selenium content is 99%.

[0026] Based on the same technical concept, another embodiment of the present invention is to provide a selenium-calcium-zinc agricultural functional active protein preparation obtained by the above preparation method.

[0027] The beneficial effects of the present invention are:

[0028] (1) The selenium-calcium-zinc agricultural functional active protein preparation obtained by the preparation method of the present invention has trace elements such as selenium, calcium, and zinc that are all converted from chemical states to active organic states through biological transformation, and has no toxic effects on the soil and the environment.

[0029] (2) After the selenium-calcium-zinc agricultural functional active protein preparation is bioconverted into an organic state, it has a high absorption efficiency when used on agricultural products, and the absorption efficiency of leaf spraying can reach more than 90%.

[0030] (3) The selenium-calcium-zinc-rich agricultural functional active protein preparation can be sprayed on leaves, is easy to use, and has low labor costs per acre of land.

[0031] (4) It can simultaneously supplement multiple trace elements such as selenium, calcium, and zinc.

[0032] (5) It can convert organic selenium that is not easily absorbed by crops into active organic selenium forms that are easily absorbed by plants, thereby promoting efficient absorption and conversion of selenium.

[0033] (6) Take into account the degradation of multiple pesticide residues and the restoration of soil.

[0034] (7) Increase crop yield and improve target organ quality. DETAILED DESCRIPTION

[0035] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0036] Example 1

[0037] This embodiment provides a method for preparing a selenium-calcium-zinc-rich agricultural functional active protein preparation, the preparation method comprising the following steps:

[0038] (1) 40 kg of licorice and 60 kg of black tea are first soaked in water for 48 hours. The first time, cold water is boiled over high heat, and then simmered over low heat for 4 hours, and 350 kg of juice is poured out; the second time, boiling water is added, and simmered over low heat for 2 hours, and 350 kg of juice is poured out; the third time, boiling water is added, and simmered over low heat for 1 hour, and 300 kg of juice is poured out. The three times together obtain 1000 kg of extract;

[0039] (2) boiling the 1000 kg extract, then adding 80 kg peptone, 40 kg yeast extract, 30 kg white sugar, and 10 kg sodium chloride, and mixing well to obtain a traditional Chinese medicine activation culture solution;

[0040] (3) lowering the temperature of the activated Chinese medicine culture solution to 45° C., adding 8 kg of red wine yeast, and fermenting under aerobic conditions at 35° C. for 7 days to obtain a first fermentation solution;

[0041] (4) At 12:00 noon on the 7th day of fermentation, 30 kg of calcium chloride and 20 kg of zinc sulfate heptahydrate were added, and fermentation was carried out under aerobic conditions at 35° C. for 7 days to obtain a second fermentation liquid;

[0042] (5) at 12:00 noon on the 14th day of fermentation, 8 kg of photosynthetic bacteria were added to the second fermentation liquid, at 12:00 noon on the 16th day of fermentation, 10 kg of actinomycetes were added, and at 12:00 noon on the 18th day of fermentation, 15 kg of Bacillus subtilis were added; starting from the 14th day, the fermentation temperature was reduced to 32° C., and the fermentation was continued with oxygen to obtain a third fermentation liquid;

[0043] (6) After 20 days of fermentation, 3 kg of organic selenium (organic selenium content 99%) was added to the third fermentation liquid. Starting from the 20th day, the fermentation temperature was reduced to 28° C. and aeration fermentation was continued until 49 days. After completion, the selenium-rich calcium-zinc agricultural functional active protein preparation was obtained.

[0044] Example 2

[0045] This embodiment provides a method for preparing a selenium-calcium-zinc-rich agricultural functional active protein preparation, the preparation method comprising the following steps:

[0046] (1) 35 kg of licorice and 55 kg of black tea are first soaked in water for 48 hours. The first time, cold water is boiled over high heat, and then simmered over low heat for 4 hours, and 350 kg of juice is poured out; the second time, boiling water is added, and simmered over low heat for 2 hours, and 350 kg of juice is poured out; the third time, boiling water is added, and simmered over low heat for 1 hour, and 300 kg of juice is poured out. The three times together obtain 1000 kg of extract;

[0047] (2) boiling the 1000 kg extract, then adding 75 kg peptone, 35 kg yeast extract, 25 kg white sugar, and 8 kg sodium chloride, and mixing well to obtain a traditional Chinese medicine activation culture solution;

[0048] (3) lowering the temperature of the activated Chinese medicine culture solution to 45° C., adding 6 kg of red wine koji, and fermenting under aerobic conditions at 35° C. for 7 days to obtain a first fermentation solution;

[0049] (4) At 12:00 noon on the 7th day of fermentation, 25 kg of calcium chloride and 15 kg of zinc sulfate heptahydrate were added, and fermentation was carried out under aerobic conditions at 35° C. for 7 days to obtain a second fermentation liquid;

[0050] (5) at 12:00 noon on the 14th day of fermentation, 6 kg of photosynthetic bacteria were added to the second fermentation liquid, at 12:00 noon on the 16th day of fermentation, 8 kg of actinomycetes were added, and at 12:00 noon on the 18th day of fermentation, 13 kg of Bacillus subtilis were added; starting from the 14th day, the fermentation temperature was reduced to 32° C., and the fermentation was continued with oxygen to obtain a third fermentation liquid;

[0051] (6) After 20 days of fermentation, 2.5 kg of organic selenium (organic selenium content 30%) was added to the third fermentation liquid. Starting from the 20th day, the fermentation temperature was reduced to 28° C. and aeration fermentation was continued until 49 days. After completion, the selenium-rich calcium-zinc agricultural functional active protein preparation was obtained.

[0052] Example 3

[0053] This embodiment provides a method for preparing a selenium-calcium-zinc-rich agricultural functional active protein preparation, the preparation method comprising the following steps:

[0054] (1) 38 kg of licorice and 58 kg of black tea were first soaked in water for 48 hours. The first time, cold water was boiled over high heat, and then simmered over low heat for 4 hours, and 350 kg of juice was poured out; the second time, boiling water was added, and simmered over low heat for 2 hours, and 350 kg of juice was poured out; the third time, boiling water was added, and simmered over low heat for 1 hour, and 300 kg of juice was poured out. The three times together obtained 1000 kg of extract;

[0055] (2) boiling the 1000 kg extract, then adding 78 kg peptone, 38 kg yeast extract, 28 kg white sugar, and 9 kg sodium chloride, and mixing well to obtain a traditional Chinese medicine activation culture solution;

[0056] (3) lowering the temperature of the activated Chinese medicine culture solution to 45° C., adding 7 kg of red wine yeast, and fermenting under aerobic conditions at 35° C. for 7 days to obtain a first fermentation solution;

[0057] (4) At 12:00 noon on the 7th day of fermentation, 28 kg of calcium chloride and 18 kg of zinc sulfate heptahydrate were added, and fermentation was carried out under aerobic conditions at 35° C. for 7 days to obtain a second fermentation liquid;

[0058] (5) at 12:00 noon on the 14th day of fermentation, 7 kg of photosynthetic bacteria were added to the second fermentation liquid, at 12:00 noon on the 16th day of fermentation, 9 kg of actinomycetes were added, and at 12:00 noon on the 18th day of fermentation, 14 kg of Bacillus subtilis were added; starting from the 14th day, the fermentation temperature was reduced to 32° C., and the fermentation was continued with oxygen to obtain a third fermentation liquid;

[0059] (6) After 20 days of fermentation, 2.8 kg of organic selenium (organic selenium content 30%) was added to the third fermentation liquid. Starting from the 20th day, the fermentation temperature was reduced to 28° C. and aeration fermentation was continued until 49 days. After completion, the selenium-rich calcium-zinc agricultural functional active protein preparation was obtained.

[0060] Verification Example

[0061] The selenium-calcium-zinc agricultural functional active protein preparation of the present invention is usually applied by 100 times foliar spraying, and root irrigation can be used for some root crops. Generally, spraying 2-3 times during the formation of the target organ can achieve the effect.

[0062] Organic selenium, organic calcium and organic zinc were detected according to the existing method, and the results are shown in Table 1.

[0063] Table 1

[0064] time Organic selenium / % Organic calcium / % Organic zinc / % 0d 0 0 0 7d 0 0 0 14d 0 34 24 21d 100 62 52 28d 100 78 81 35d 100 89 90 42d 100 96 96 49d 100 100 100

[0065] As shown in Table 1, the biotransformation of selenium, calcium and zinc through the fermentation process achieved 100% organic active conversion.

[0066] Example 1 of the present invention (this product) and relevant commercial products (comparative products A, B, and C) were used as test objects, and tomatoes, peaches, and corns were sprayed, and their effects were tested.

[0067] The spraying effect on tomatoes is shown in Table 2.

[0068] Table 2

[0069]

[0070] The spraying method of this product is: spray after 100 times dilution; the comparison product A is: organic selenium is diluted 300 times and sprayed; the comparison product B is: calcium chloride is diluted 300 times and sprayed; the comparison product C is: zinc sulfate heptahydrate is diluted 300 times and sprayed; the same below.

[0071] The spraying effect on peach is shown in Table 3.

[0072] Table 3

[0073]

[0074] The spraying effect on corn is shown in Table 4.

[0075] Table 4

[0076]

[0077] It can be seen from Tables 2 to 4 that the effects of the products of the present invention are significantly better than similar products on the market.

[0078] The temperature in Shandong Province reached a maximum of 32 degrees and a minimum of 20 degrees at the end of May. The main pesticide mixture (1000 times of phoxim (45% phoxim emulsifiable concentrate), 1000 times of phorate (55% phorate), 200 times of triazophos (4% triazophos), 500 times of thiamethoxam (25% thiamethoxam), 500 times of imidacloprid (20% concentrated soluble powder)) was irrigated to the roots of tomatoes, peaches and corns for pesticide residue detection. 1000 jin / mu, half a month after irrigating the roots, 200 times of the embodiment 1 (this product) of the present invention was irrigated 2 times every 7 days, and 300 times of the embodiment 1 (this product) of the present invention was sprayed on the leaves every 2 days, and 1000 jin of the embodiment 1 (this product) of the present invention was irrigated 3 times. Soil and target organs (tomato fruits, peach fruits, corn cobs) were taken as detection objects every 2 days, and their pesticide residues were detected. The results are shown in Table 5.

[0079] Table 5

[0080]

[0081] Table 5 shows that the pesticide residues in the soil of tomatoes, peaches and corns were basically completely degraded about half a month after the application of this product. Under the condition that the pesticide residues of phoxim, phorate, triazophos, thiamethoxam and imidacloprid in tomato fruits, peach fruits and corn kernels exceeded the national standard by 400 times, the pesticide residues were reduced to below the national limit on the 4th day after the use of this product, and the pesticide residues were reduced to 0 on the 10th day.

[0082] Table 6

[0083]

[0084] It can be seen from Table 6 that the application of Example 1 of the present invention (this product) can increase soil bulk density, maintain acid-base balance, increase soil organic matter, and increase soil microbial biomass carbon, thereby repairing the soil.

[0085] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A method for preparing a selenium-calcium-zinc-rich agricultural functional active protein preparation, characterized in that: The preparation method comprises the following steps: (1) mixing liquorice and black tea, soaking the mixture in water, and then heating and extracting the mixture to obtain an extract; (2) heating the extract and then sequentially adding peptone, yeast extract, white sugar, and sodium chloride, and mixing them evenly to obtain a traditional Chinese medicine activation culture solution; (3) adding red wine yeast to the activated Chinese medicine culture solution, and fermenting under aerobic conditions to obtain a first fermentation solution; (4) adding calcium chloride and zinc sulfate heptahydrate to the first fermentation liquid in sequence, and continuing the fermentation under aerobic conditions to obtain a second fermentation liquid; (5) adding photosynthetic bacteria, actinomycetes, and Bacillus subtilis to the second fermentation liquid in sequence, and continuing fermentation under aerobic conditions to obtain a third fermentation liquid; (6) adding organic selenium to the third fermentation liquid, and continuing the fermentation under aerobic conditions, to obtain the selenium-rich calcium-zinc agricultural functional active protein preparation.

2. The method for preparing the selenium-calcium-zinc agricultural functional active protein preparation according to claim 1, characterized in that: In step (1), the heating and leaching are performed three times; wherein: The first extraction was carried out under boiling conditions for 4 hours; The second extraction was carried out under boiling conditions for 2 hours; The third extraction was carried out under boiling conditions for 1 h.

3. The method for preparing the selenium-calcium-zinc agricultural functional active protein preparation according to claim 1, characterized in that: In step (1), the raw materials are used in the following amounts by weight: 35-40 parts of liquorice and 55-60 parts of black tea.

4. The method for preparing the selenium-calcium-zinc agricultural functional active protein preparation according to claim 1, characterized in that: In step (2), the amounts of raw materials used are, by weight, 75-80 parts of peptone, 35-40 parts of yeast extract, 25-30 parts of white sugar, and 8-10 parts of sodium chloride.

5. The method for preparing the selenium-calcium-zinc agricultural functional active protein preparation according to claim 1, characterized in that: In step (3), the amount of raw materials used is, by weight: 6 to 8 parts of red wine koji.

6. The method for preparing the selenium-calcium-zinc agricultural functional active protein preparation according to claim 1, characterized in that: In step (4), the amounts of raw materials used are, by weight, 25 to 30 parts of calcium chloride and 15 to 20 parts of zinc sulfate heptahydrate.

7. The method for preparing the selenium-calcium-zinc agricultural functional active protein preparation according to claim 1, characterized in that: In step (5), the amounts of raw materials used are, by weight, 6 to 8 parts of photosynthetic bacteria, 8 to 10 parts of actinomycetes, and 13 to 15 parts of Bacillus subtilis.

8. The method for preparing the selenium-calcium-zinc agricultural functional active protein preparation according to claim 1, characterized in that: In step (6), the amount of raw materials used is, by weight: 3 parts of organic selenium; the organic selenium content is 99%.

9. The selenium-calcium-zinc agricultural functional active protein preparation obtained by the preparation method according to any one of claims 1 to 8.

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