Cell sorting system and method and device for adjusting interdigital transducer

Abstract

The invention provides a cell sorting system and an interdigital transducer adjusting method and device.Before specific implementation, the cell sorting system can be improved firstly, specifically, feature information collecting units are arranged at the corresponding positions of a first interdigital transducer, a second interdigital transducer and a third interdigital transducer respectively; meanwhile, a feedback module and a control module are also introduced. During specific implementation, in the process of sorting and processing the target cells based on the cell sorting system, the characteristic information about the interdigital transducer is obtained and utilized through the feedback module, and whether the interdigital transducer in the cell sorting system meets the operation requirement or not is automatically detected and judged. After the target interdigital transducer which does not meet the operation requirements is identified, the excitation parameters of the target interdigital transducer can be adjusted in a targeted manner according to the feature information of the target interdigital transducer, so that the interdigital transducers in the cell sorting system can accurately operate according to the expected operation requirements, and the accuracy of cell sorting is improved. And a better cell sorting effect is obtained.

Description

Technical Field

[0001] The present invention relates to the field of biological ultrasonic instrument technology, and in particular to a cell sorting system, an adjustment method and device for an interdigital transducer. Background Art

[0002] In the field of biotechnology, cell sorting equipment is often used to screen the required cells using ultrasound technology. Among them, the above-mentioned interdigital transducer is a key component in the cell sorting equipment, and the actual operation of the interdigital transducer will have an important impact on the cell sorting effect of the cell sorting equipment.

[0003] However, based on existing equipment and methods, it is often impossible to automatically detect and analyze the actual operating conditions of the interdigital transducer, resulting in less than ideal cell sorting effects when using a cell sorting device to sort and process cells.

[0004] To address the above problems, no effective solution has been proposed yet. Summary of the invention

[0005] The present specification provides a method and device for adjusting a cell sorting system and an IDT, which can automatically detect and determine whether the IDT in the cell sorting system meets the operating requirements; and can also specifically adjust the excitation parameters of the target IDT that does not meet the operating requirements, so that the IDT in the cell sorting system can accurately operate according to the expected operating requirements and obtain better cell sorting effects.

[0006] This specification provides a cell sorting system, which at least includes: a cell transport pipeline, a feedback module, an excitation module, and a control module; wherein:

[0007] The end of the cell transport pipeline is connected to at least a first sub-pipeline and a second sub-pipeline; and a cell arrangement module, a cell squeezing module, a cell positioning module, and a cell sorting module are sequentially arranged along the cell transport pipeline;

[0008] The cell arrangement module includes at least a first interdigital transducer, the cell squeezing module includes at least a second interdigital transducer, and the cell sorting module includes at least a third interdigital transducer; wherein the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer are respectively connected to an excitation module; and the excitation module is connected to a control module;

[0009] Matching feature information acquisition units are also respectively arranged at corresponding positions of the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer;

[0010] The characteristic information acquisition unit is connected to a feedback module, and the feedback module is used to acquire characteristic information about the interdigital transducer through the characteristic information acquisition unit;

[0011] The control module is connected to the feedback module, and the feedback module is also used to send the characteristic information to the control module;

[0012] The control module is used to detect and determine a target IDT that does not meet the operating requirements from the first IDT, the second IDT, and the third IDT according to the characteristic information; and adjust the excitation parameters of the target IDT through the excitation module.

[0013] In one embodiment, the feature information acquisition unit includes at least one of the following: a microscopic camera, a temperature sensor, and a power detection circuit.

[0014] In one embodiment, the third IDT includes a plurality of sub-IDTs.

[0015] This specification also provides an adjustment method of an interdigital transducer, comprising:

[0016] Acquiring characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells; wherein the characteristic information at least carries a device identification of the corresponding interdigital transducer;

[0017] According to the device identification, the characteristic information is divided into a plurality of characteristic information data groups; wherein one characteristic information data group corresponds to one interdigital transducer;

[0018] According to the device identification, the operation rule of the corresponding interdigital transducer and the sound field type of the sound field provided by the interdigital transducer are determined;

[0019] According to the operation rules and acoustic field types of the IDT, by processing the corresponding characteristic information data group, detecting whether the IDT in the cell sorting system meets the operation requirements;

[0020] In the case where it is determined that there is an IDT that does not meet the operation requirements, the IDT that does not meet the operation requirements is determined as a target IDT;

[0021] According to the characteristic information data set of the target IDT, the excitation parameters of the target IDT are adjusted.

[0022] In one embodiment, the characteristic information includes at least one of the following: temperature information, image information, and power information.

[0023] In one embodiment, adjusting the excitation parameters of the target IDT according to the characteristic information data set of the target IDT includes:

[0024] According to the device identification of the target interdigital transducer, a matching target processing rule is determined;

[0025] Determine the problem factors that cause the target interdigital transducer to fail to meet the operation requirements according to the target processing rules and the characteristic information data group of the target interdigital transducer;

[0026] Determine the matching target adjustment strategy based on the problem factors, the operating rules of the target IDT and the sound field type;

[0027] According to the target adjustment strategy, the excitation parameters of the target IDT are adjusted.

[0028] In one embodiment, before acquiring characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells, the method further includes:

[0029] Starting a cell sorting system, and using the cell sorting system to sort and process test cells;

[0030] Acquiring test information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing test cells;

[0031] According to the test rules, using the test information, the cell sorting system is initially tested to obtain corresponding test results;

[0032] According to the test results, when it is determined that at least one IDT in the cell sorting system does not meet the operation requirements, the IDT is initially adjusted accordingly.

[0033] This specification also provides an excitation system for an IDT, comprising at least: a control module, an IDT, a feedback module, and an excitation module; wherein the excitation module is connected to the IDT;

[0034] The feedback module is used to collect characteristic information related to the interdigital transducer;

[0035] The control module is used to detect whether the IDT meets the operation requirements according to the characteristic information; and if it is determined that the IDT does not meet the operation requirements, adjust the excitation parameters of the IDT through the excitation module.

[0036] This specification also provides an adjustment device for an interdigital transducer, comprising:

[0037] An acquisition module, used to acquire characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells; wherein the characteristic information at least carries a device identification of the corresponding interdigital transducer;

[0038] A division module, used for dividing the characteristic information into a plurality of characteristic information data groups according to the device identification; wherein one characteristic information data group corresponds to one interdigital transducer respectively;

[0039] A first determination module is used to determine the operation rule of the corresponding IDT and the sound field type of the sound field provided by the IDT according to the device identification;

[0040] A detection module, used to detect whether the interdigital transducer in the cell sorting system meets the operation requirements by processing the corresponding characteristic information data group according to the operation rules and acoustic field type of the interdigital transducer;

[0041] A second determination module is used to determine the IDT that does not meet the operation requirements as a target IDT when it is determined that there is an IDT that does not meet the operation requirements;

[0042] The adjustment module is used to adjust the excitation parameters of the target IDT according to the characteristic information data group of the target IDT.

[0043] The present specification also provides a computer-readable storage medium on which computer instructions are stored. When the instructions are executed by a processor, the relevant steps of the IDT adjustment method are implemented.

[0044] Based on the cell sorting system, the adjustment method and device of the interdigital transducer provided in this specification, before the specific implementation, the cell sorting system can be improved first: matching feature information acquisition units are respectively set at the corresponding positions of the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer; at the same time, a feedback module and a control module are also introduced, and the feedback module is connected to the feature information acquisition unit and the control module respectively, and the control module is connected to the excitation module acting on the interdigital transducer. In the specific implementation, in the process of sorting and processing target cells based on the above-mentioned cell sorting system, the feature information about the interdigital transducer is first obtained; wherein the above-mentioned feature information at least carries the device identification of the corresponding interdigital transducer; according to the device identification, the feature information is divided into a plurality of feature information data groups corresponding to different interdigital transducers; at the same time, according to the device identification, the operation rules of the corresponding interdigital transducer and the type of the sound field are determined; then according to the operation rules and the type of the sound field of the interdigital transducer, different interdigital transducers are distinguished, and by processing the corresponding feature information data group, it is automatically detected and determined whether the interdigital transducer in the cell sorting system meets the operation requirements. When it is determined that there are IDTs in the cell sorting system that do not meet the operating requirements, different IDTs can be distinguished based on the characteristic information of the target IDT that does not meet the operating requirements, and the excitation parameters of the target IDT can be adjusted in a targeted manner so that the IDTs in the cell sorting system can operate accurately according to the expected operating requirements, thereby obtaining better cell sorting effects and effectively reducing the damage to the cells during the sorting and processing of the target cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] In order to more clearly illustrate the embodiments of this specification, the drawings required for use in the embodiments will be briefly introduced below. The drawings described below are only some embodiments recorded in this specification. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0046] Figure 1 is a schematic diagram of the structure of a cell sorting system provided in one embodiment of the present specification;

[0047] Figure 2 is a flow chart of an adjustment method of an interdigital transducer provided by an embodiment of the present specification;

[0048] Figure 3 is a schematic diagram of an embodiment of an IDT adjustment method provided by an embodiment of this specification, in a scenario example;

[0049] Figure 4 is a schematic diagram of an embodiment of an IDT adjustment method provided by an embodiment of this specification, in a scenario example;

[0050] Figure 5 is a schematic diagram of the structure of an excitation system of an interdigital transducer provided by an embodiment of the present specification;

[0051] Figure 6 is a schematic diagram of the structure of an electronic device provided by an embodiment of this specification;

[0052] Figure 7 is a schematic diagram of the structure of an adjustment device for an interdigital transducer provided by an embodiment of the present specification;

[0053] Figure 8 is a schematic diagram of an embodiment of an IDT adjustment method provided by an embodiment of this specification, in a scenario example;

[0054] Fig. 9 is a schematic diagram of an embodiment of an IDT adjustment method provided by an embodiment of this specification, in a scenario example;

[0055] Fig.10 It is a schematic diagram of an embodiment of an IDT adjustment method provided by an embodiment of this specification, in a scenario example. DETAILED DESCRIPTION

[0056] In order to enable those skilled in the art to better understand the technical solutions in this specification, the technical solutions in the embodiments of this specification will be clearly and completely described below in conjunction with the drawings in the embodiments of this specification. Obviously, the described embodiments are only part of the embodiments of this specification, not all of the embodiments. Based on the embodiments in this specification, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of this specification.

[0057] It should be noted that in the embodiments of this specification, certain software, components, models and other existing solutions in the industry may be mentioned, and they should be regarded as exemplary. Their purpose is only to illustrate the feasibility of implementing the technical solution of this application, but it does not mean that the applicant has or will necessarily use the solution.

[0058] See also Figure 1 As shown, the embodiment of this specification provides a cell sorting system, which may at least include: a cell transport pipeline, a feedback module, an excitation module, and a control module; wherein,

[0059] The end of the cell transport pipeline is connected to at least a first sub-pipeline and a second sub-pipeline; and a cell arrangement module, a cell squeezing module, a cell positioning module, and a cell sorting module are sequentially arranged along the cell transport pipeline;

[0060] The cell arrangement module includes at least a first interdigital transducer, the cell squeezing module includes at least a second interdigital transducer, and the cell sorting module includes at least a third interdigital transducer; wherein the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer are respectively connected to an excitation module; and the excitation module is connected to a control module;

[0061] Matching feature information acquisition units are also respectively arranged at corresponding positions of the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer;

[0062] The characteristic information acquisition unit is connected to a feedback module, and the feedback module is used to acquire characteristic information about the interdigital transducer through the characteristic information acquisition unit;

[0063] The control module is connected to the feedback module, and the feedback module is also used to send the characteristic information to the control module;

[0064] The control module is used to detect and determine a target IDT that does not meet the operating requirements from the first IDT, the second IDT, and the third IDT according to the characteristic information; and adjust the excitation parameters of the target IDT through the excitation module.

[0065] The first, second and third IDTs can be understood as IDTs deployed in different functional modules of the cell sorting system to implement different operations. The first, second and third IDTs can provide different types of sound fields.

[0066] The above-mentioned interdigital transducer (IDT) can specifically be a device made by depositing a layer of metal film on a piezoelectric substrate and then obtaining a row of crossed electrodes by photolithography. Based on the interdigital transducer, when an alternating electric field is loaded on a row of crossed electrodes, surface acoustic waves can be generated on the piezoelectric substrate through the reverse voltage effect to form a corresponding sound field. The above-mentioned interdigital transducer has the characteristics of simple production, high acoustic-to-electric conversion efficiency, high working efficiency, ability to eliminate parasitic modes, and superior performance. In addition, the above-mentioned interdigital transducer has broad application prospects and development space. For example, it can be applied to various acoustic and acoustic-to-electric conversion occasions, such as surface acoustic wave devices, ultrasonic sensors, etc. Moreover, with the continuous development of science and technology, the application fields of interdigital transducers are also constantly expanding. For example, interdigital transducers can be applied to biomedicine, ultrasonic imaging, etc.; they can also be applied to the field of wireless communications to realize acoustic wave communication, etc.

[0067] The target cells may be specifically understood as cells to be sorted. Specifically, the target cells may include cells in a state to be sorted (eg, healthy state) and / or cells of a type to be sorted (eg, blood cells).

[0068] The cell arrangement module at least includes a first interdigital transducer. In specific implementation, the cell arrangement module can use the first interdigital transducer to generate a standing wave acoustic field for the mixed cells containing target cells transported through the cell transport channel, so that the randomly stacked mixed cells are arranged in a straight line and transported in the cell transport channel in the form of a straight line. The thickness of the straight line can be 1 cell.

[0069] The cell squeezing module at least includes a second interdigital transducer. In specific implementation, the cell squeezing module can use the second interdigital transducer to generate a focused acoustic field for the mixed cells arranged in a straight line transported through the cell transport pipeline, so as to generate radiation force on the mixed cells to cause the cells to deform; then receive and identify the target cells to be sorted from the mixed cells based on the deformation information of the cells.

[0070] The cell positioning module may specifically include: a photosensitive electrode. In specific implementation, the cell positioning module may use the photosensitive electrode to collect the speed information of the target cell of interest and the distance information between the target cell and the third interdigital transducer in the cell sorting module; calculate the arrival time of the target cell when it reaches the third interdigital transducer in the cell sorting module according to the speed information and distance information of the target cell; and send the arrival time to the cell sorting module in advance.

[0071] The cell sorting module at least includes a third interdigital transducer. In specific implementation, when the cell sorting module detects that the current time is the arrival time of the target cell, the third interdigital transducer can be used to generate a planar acoustic field for the target cell transported through the cell transport pipeline, so as to exert a force on the target cell, change the movement speed and direction of the target cell, and then change the movement trajectory of the target cell, so that the target cell deviates from the movement trajectory of the original mixed cell, for example, the target cell eventually moves in the direction of the first sub-pipeline at the end of the cell transport pipeline according to the changed movement trajectory, and enters the first sub-pipeline; at the same time, the remaining cells in the original mixed cell except the target cell continue to move in the direction of the second sub-pipeline at the end of the cell transport pipeline according to the original movement trajectory, and then the second sub-pipeline, thereby realizing cell sorting.

[0072] In a specific implementation, based on the above-mentioned cell sorting system, in the process of sorting the target cells in the above-mentioned manner, the feedback module can collect characteristic information related to the interdigital transducer through the characteristic information acquisition unit as feedback information; and then analyze and detect the actual operating conditions of the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer based on the above-mentioned characteristic information to determine whether the above-mentioned interdigital transducers meet the operating requirements.

[0073] When it is determined that the first IDT, the second IDT, and the third IDT all meet the operating requirements, it can be determined that the IDTs in the current cell sorting system all operate according to the expected operating requirements, and further it can be determined that a good cell sorting effect can be obtained at present. At this time, there is no need to adjust the excitation parameters of the IDTs.

[0074] On the contrary, when it is determined that at least one of the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer does not meet the operating requirements, it can be determined that some interdigital transducers in the current cell sorting system do not operate according to the expected operating requirements, and then it can be judged that it is highly likely that a good cell sorting effect cannot be obtained at present. At this time, the interdigital transducer that does not meet the operating requirements can be determined as the target interdigital transducer; then, according to the characteristic information of the target interdigital transducer, the control module is used to adjust the excitation parameters of the target interdigital transducer through the excitation module, so that the target interdigital transducer meets the expected requirements again, thereby ensuring a good cell sorting effect.

[0075] Based on the above-mentioned cell sorting system, it is possible to automatically detect and determine whether the interdigital transducer in the cell sorting system meets the operating requirements; and when it is determined that there is an interdigital transducer in the cell sorting system that does not meet the operating requirements, it is also possible to adjust the excitation parameters of the target interdigital transducer in a targeted manner based on the characteristic information of the target interdigital transducer that does not meet the operating requirements, so that the interdigital transducer in the cell sorting system can operate accurately according to the expected operating requirements, thereby obtaining a better cell sorting effect and effectively reducing the damage to cells in the related process.

[0076] In some embodiments, the feature information acquisition unit may specifically include at least one of the following: a microscopic camera, a temperature sensor, a power detection circuit, etc.

[0077] In a specific implementation, a microscopic camera can be respectively arranged at the positions adjacent to the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer inside the cell transport channel, for respectively collecting image information about cells at positions adjacent to the relevant interdigital transducers as a kind of characteristic information. Of course, considering that the internal space of the cell transport channel is relatively limited, only one microscopic camera can be arranged at the middle position inside the cell transport channel; the microscopic camera can collect image information about cells at positions adjacent to the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer.

[0078] In specific implementation, corresponding power detection circuits can be arranged at the corresponding interfaces of the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer respectively to collect power information such as forward power and reverse power of each interdigital transducer as another type of characteristic information.

[0079] In a specific implementation, a temperature sensor can be respectively arranged inside the cell transport channel near the first, second and third IDTs to collect temperature information about cells near the relevant IDTs as another characteristic information.

[0080] In addition, during specific implementation, other types of characteristic information such as temperature information about the IDT, image information about the IDT, etc. may also be collected through a related characteristic information collection unit.

[0081] Based on the above embodiments, relatively rich and comprehensive feature information can be collected by the corresponding feature information collection unit, so that the relevant interdigital transducers can be detected and adjusted more accurately based on the above feature information.

[0082] In some embodiments, the third IDT may specifically include a plurality of sub-IDTs.

[0083] For example, see Figure 1 As shown, the third IDT may include three sub-IDTs arranged at equal intervals, which are respectively marked as: sub-IDT 1, sub-IDT 2, and sub-IDT 3.

[0084] When specifically sorting and processing target cells, the cell sorting module can use a combination of multiple sub-interdigital transducers to generate a planar acoustic field and apply force to the target cells multiple times at different time points, so as to more accurately control the target cells to enter the corresponding sub-pipeline (for example, the first sub-pipeline or the second sub-pipeline) to complete cell sorting.

[0085] Correspondingly, the control module can also collect feedback and, based on multiple characteristic information about the sub-interdigital transducers, comprehensively analyze and detect whether the third interdigital transducer composed of multiple sub-interdigital transducers meets the operating requirements as a whole; and when it is determined that it does not meet the operating requirements, comprehensively utilize multiple characteristic information about the sub-interdigital transducers to jointly adjust the excitation parameters of the multiple sub-interdigital transducers so that the third interdigital transducer as a whole can meet the operating requirements.

[0086] In some embodiments, the control module may specifically include an FPGA (Field Programmable Gate Array) main control module.

[0087] The cell sorting system may further include a power module, wherein the power module is used to provide the required electrical energy for the cell sorting system, and specifically, the power module may be connected to the control module.

[0088] The cell sorting system may further include an interactive module, such as a touch screen, a control panel, a control button, and other devices. In specific implementation, the user may use the interactive module to interact with the cell sorting system to input custom information. Accordingly, the cell sorting system may receive the user's custom information through the interactive module, and in combination with the user's custom information, realize the detection and adjustment of the related interdigital transducers to meet the diverse needs of the user.

[0089] The above-mentioned excitation module may specifically include: a current-to-voltage circuit, a filter circuit, a signal amplification circuit, a power amplification circuit and other circuit structures.

[0090] In specific implementation, the control module can, according to design requirements, first use the current-to-voltage circuit in the excitation module to convert the current signal output by the power module into a corresponding voltage signal; then use the filter circuit to perform corresponding filtering processing on the voltage signal to obtain a filtered voltage signal; use the signal amplification circuit to perform corresponding signal amplification processing on the filtered voltage signal to obtain an amplified signal; finally, use the power amplification circuit to perform corresponding power amplification on the amplified signal to obtain a power-amplified signal. The above-mentioned power-amplified signal can then be fed into the corresponding interdigital transducer so that the interdigital transducer provides a corresponding sound field.

[0091] See also Figure 2 As shown, this specification also provides an adjustment method of the interdigital transducer based on the above cell sorting system. When the method is implemented specifically, it may include the following contents:

[0092] S201: Acquire characteristic information about the IDT collected by the cell sorting system during the process of sorting and processing target cells; wherein the characteristic information at least carries a device identification of the corresponding IDT;

[0093] S202: dividing the characteristic information into a plurality of characteristic information data groups according to the device identification; wherein each characteristic information data group corresponds to one interdigital transducer;

[0094] S203: determining, according to the device identification, an operation rule of the corresponding IDT and a sound field type of the sound field provided by the IDT;

[0095] S204: According to the operation rules and acoustic field types of the IDT, by processing the corresponding characteristic information data group, detecting whether the IDT in the cell sorting system meets the operation requirements;

[0096] S205: in the case where it is determined that there is an IDT that does not meet the operation requirement, determining the IDT that does not meet the operation requirement as a target IDT;

[0097] S206: Adjusting the excitation parameters of the target IDT according to the characteristic information data set of the target IDT.

[0098] In specific implementation, the above method can be applied to the control module side of the cell sorting system. In the actual process of sorting and processing target cells by the cell sorting system, the characteristic information about the interdigital transducer is automatically collected through the feedback module.

[0099] The characteristic information may be characteristic information for one IDT, and accordingly, only carries the device identification of one IDT; or characteristic information for multiple IDTs, and accordingly, carries the device identification of multiple IDTs. The device identification corresponds to one IDT.

[0100] The above-mentioned IDT may specifically include: different types of IDTs such as a first IDT, a second IDT, and a third IDT, etc. Among them, the sound field types of the sound fields provided by different IDTs may be different.

[0101] In specific implementation, the collected characteristic information can be divided into multiple characteristic information data groups according to the device identification; each characteristic information data group corresponds to an IDT and contains one or more characteristic information related to the IDT.

[0102] At the same time, according to the device identification, the preset operation rule library can be queried to determine a preset operation rule that matches the device identification as the corresponding operation rule of the interdigital transducer. Among them, the preset operation rule library can store multiple preset operation rules, each preset operation rule at least includes the operation requirements of the corresponding interdigital transducer, and the detection characteristics and detection methods required to detect the operation requirements, etc., and each preset operation rule corresponds to a device identification of an interdigital transducer.

[0103] Before specific implementation, the design requirements for different types of IDTs in the cell sorting system can be combined with the experimental detection data of the cell sorting system under normal conditions to learn and organize multiple preset operating rules corresponding to different IDTs to establish a preset operating rule library.

[0104] In addition, the function usage information table of the interdigital transducer of the cell sorting system can be queried according to the device identification to determine the sound field type of the sound field provided by the interdigital transducer corresponding to the device identification in the cell sorting system. Among them, the above-mentioned sound field type can include at least one of the following: standing wave sound field, focused sound field, planar sound field, etc. Of course, it should be noted that the above-mentioned sound field types are only a schematic description. In specific implementation, other sound field types can also be included according to specific application scenarios and processing requirements. This manual does not limit this.

[0105] Furthermore, the cell type of the target cell currently being sorted by the cell sorting system may also be obtained.

[0106] In a specific implementation, according to the operating rules of each IDT, combined with the type of sound field provided by the IDT, and the cell type of the target cells targeted by the IDT, by processing the characteristic information contained in the corresponding characteristic information data group, different IDTs in the cell sorting system, such as the first IDT, the second IDT, the third IDT, etc., can be detected to see whether they meet the operating requirements, and corresponding detection results can be obtained.

[0107] According to the detection result, when it is determined that each IDT meets the operation requirements, the excitation parameters used by the current excitation module for each IDT may be maintained without adjustment.

[0108] On the contrary, according to the detection results, when it is determined that at least one of the multiple IDTs does not meet the operating requirements, the IDT that does not meet the operating requirements can be determined as the target IDT; then, based on the device identification of the target IDT, different IDTs can be distinguished, and the characteristic information data of the target IDT can be used in a matching data processing method to adjust the excitation parameters of the target IDT in a targeted manner to obtain a relatively good cell sorting effect.

[0109] Based on the above embodiments, characteristic information about the IDT can be obtained and used as feedback information; different IDTs can be distinguished, and according to the corresponding operating rules, relevant characteristic information data groups can be used to accurately detect and determine whether each IDT in the cell sorting system meets the operating requirements; and when a target IDT that does not meet the operating requirements is detected, the excitation parameters of the target IDT can be adjusted in a targeted manner so that the target IDT meets the operating requirements, thereby obtaining a better cell sorting effect.

[0110] In some embodiments, the characteristic information may specifically include at least one of the following: temperature information, image information, power information, and the like.

[0111] In a specific implementation, the collected characteristic information about the IDT may include characteristic information of the IDT itself and / or characteristic information of target cells targeted by the IDT.

[0112] In some embodiments, considering the differences in the types of sound fields provided and the differences in the functional roles played by different interdigital transducers, the main feature information contained in the corresponding feature information data sets may also be different.

[0113] Specifically, for the first interdigital transducer, the corresponding characteristic information data group may at least include: a first image (a type of image information) of mixed cells containing target cells after the stationary sound field arrangement provided by the first interdigital transducer, and the temperature of the mixed cells (a type of temperature information).

[0114] For the second IDT, the corresponding characteristic information data set may include at least: a second image (another image information) of the mixed cells squeezed by the focused acoustic field provided by the second IDT, and power information (a power information) of the second IDT.

[0115] For the third interdigital transducer, the corresponding characteristic information data set may at least include: a third image of the target cell after being acted upon by the planar acoustic field provided by the third interdigital transducer (another image information), the temperature of the target cell (another temperature information), and the power transmission efficiency of the third interdigital transducer (another power information). It should be noted that the third image here may specifically include two images collected at two different time points with a fixed time interval (e.g., 2 seconds) after being acted upon by the planar acoustic field.

[0116] Specifically, when obtaining the power transmission efficiency, the corresponding characteristic information collection unit may be used to respectively collect the forward power and reverse power of the third interdigital transducer; and then the forward power and reverse power may be used to calculate the corresponding power transmission efficiency.

[0117] In specific implementation, considering that the first interdigital transducer provides a standing wave acoustic field, the emitted wave is a continuous wave, which generates relatively more heat; and its function is to make the mixed cells containing the target cells line up in a straight line. Accordingly, according to the operation rules and acoustic field types of the interdigital transducer, by processing the corresponding characteristic information data group, the interdigital transducer in the cell sorting system is detected to see whether it meets the operation requirements. In specific implementation, the following contents may be included:

[0118] S1: according to the operation rule of the first interdigital transducer, calling the first image recognition model to perform image recognition on the first image, so as to determine and mark the cell position point in the first image; wherein the cell position point is the center point of the cell in the image;

[0119] S2: respectively calculating the distance difference between two adjacent cell position points in the vertical direction to the cell transport channel; and detecting whether there is a distance difference greater than a preset first distance threshold;

[0120] S3: When it is determined that there is at least one distance difference greater than a preset first distance threshold, it can be determined that the hybrid cells are not accurately arranged in a straight line under the sound field provided by the first interdigital transducer, and further it can be determined that the first interdigital transducer does not meet the operating requirements.

[0121] On the contrary, when it is determined that there is no distance difference greater than the preset first distance threshold, the average value and variance of the distance difference can be further calculated; and it is detected whether the average value of the distance difference is greater than the preset second distance threshold, and whether the variance is greater than the preset variance threshold; wherein the preset second distance threshold is smaller than the preset first distance threshold.

[0122] When it is determined that the average value of the distance difference is greater than the preset second distance threshold, and the variance is greater than the preset variance threshold, it can be determined that the first interdigital transducer does not meet the operating requirements; on the contrary, when it is determined that the average value of the distance difference is less than or equal to the preset second distance threshold, or the variance is less than or equal to the preset variance threshold, it can be further detected whether the temperature of the mixed cell is greater than the preset first temperature threshold.

[0123] When it is determined that the temperature of the hybrid cell is greater than the preset first temperature threshold, it can be determined that the temperature is relatively too high and may affect the activity of the hybrid cell, and further it can be determined that the first interdigital transducer does not meet the operating requirements.

[0124] On the contrary, when it is determined that the temperature of the mixed cell is less than or equal to the preset first temperature threshold, it can be determined that the first interdigital transducer meets the operation requirements.

[0125] Based on the above embodiments, it is possible to accurately detect and determine whether the first interdigital transducer meets the operating requirements according to the corresponding operating rules.

[0126] In the specific implementation, considering that the sound field provided by the second interdigital transducer is a focused sound field, the emitted wave is a pulse wave; and its function is to apply force to the mixed cells to generate a deformation amount, and then obtain and determine the target cells from the mixed cells based on the deformation amount. Accordingly, according to the operation rules and sound field type of the interdigital transducer, by processing the corresponding characteristic information data group, whether the interdigital transducer in the cell sorting system meets the operation requirements, the specific implementation may include the following contents:

[0127] S1: according to the operation rule of the second interdigital transducer, calling the second image recognition model to perform image recognition on the second image, and determining the feedback characteristics of the cells subjected to the force in the mixed cells; wherein the feedback characteristics of the cells include: the deformation position of the cells, the deformation amount of the cells, etc.;

[0128] S2: according to the feedback characteristics of the cells subjected to the force in the mixed cells, in combination with the power information (for example, output power) of the second interdigital transducer, a preset feedback characteristic template of the reference cells is queried to determine the target cells;

[0129] S3: Detecting whether the deformation amount of the target cell is greater than a first deformation amount threshold; wherein the first deformation amount threshold is determined according to power information of the second interdigital transducer;

[0130] S4: When it is determined that the deformation amount of the target cell is greater than the first deformation amount threshold, it can be determined that the force actually acting on the target cell is too large, and further it can be determined that the second interdigital transducer does not meet the operation requirements.

[0131] On the contrary, when it is determined that the deformation amount of the target cell is less than or equal to the first deformation amount threshold, it can be further detected whether the deformation amount of the target cell is less than the second deformation amount threshold; wherein the second deformation amount threshold can be a smaller value than the first deformation amount threshold.

[0132] When it is determined that the deformation amount of the target cell is less than the second deformation amount threshold, it can be determined that the force actually acting on the target cell is too small, and further it can be determined that the second interdigital transducer does not meet the operation requirements.

[0133] On the contrary, when it is determined that the deformation amount of the target cell is greater than or equal to the second deformation amount threshold, it can be determined that the second interdigital transducer meets the operation requirements.

[0134] Based on the above embodiments, it is possible to accurately detect and determine whether the second IDT meets the operating requirements according to the corresponding operating rules.

[0135] In the specific implementation, considering that the sound field provided by the third interdigital transducer is a planar sound field, the emitted wave is a pulse wave; and its function is to exert force on the target cell, so that the movement of the target cell is offset, thereby changing the movement trajectory, so as to distinguish it from other cells and enter the designated sub-pipeline. Accordingly, according to the operation rules and sound field type of the interdigital transducer, by processing the corresponding characteristic information data group, whether the interdigital transducer in the cell sorting system meets the operation requirements, the specific implementation may include the following contents:

[0136] S1: according to the operation rule of the third interdigital transducer, calling the third image recognition model to perform image recognition on the third image, so as to determine the current position and current speed of the target cell after being acted upon by the third interdigital transducer; wherein the current speed includes the current speed direction and the current speed value;

[0137] S2: marking the current position of the target cell and the current speed of the target cell in the third image to obtain a marked third image;

[0138] S3: Processing the marked third image using the trajectory prediction model pre-trained by machine learning to obtain a corresponding prediction result;

[0139] S4: According to the prediction result, when it is determined that the target cells cannot normally enter the designated sub-pipeline after being acted upon by the third interdigital transducer, it is determined that the third interdigital transducer does not meet the operation requirements.

[0140] On the contrary, when it is determined based on the prediction results that the target cells can normally enter the designated sub-pipeline after being acted upon by the third interdigital transducer, it is possible to further detect whether the power transmission efficiency of the third interdigital transducer is within the preset transmission efficiency range; at the same time, detect whether the temperature of the target cells is less than the preset second temperature threshold.

[0141] If it is determined that the power transmission efficiency of the third IDT falls within the preset transmission efficiency range and the temperature of the target cell is less than the preset second temperature threshold, it can be determined that the third IDT meets the operation requirements. Otherwise, it can be determined that the third IDT does not meet the operation requirements.

[0142] Based on the above embodiments, it is possible to accurately detect and determine whether the third interdigital transducer meets the operating requirements according to the corresponding operating rules.

[0143] In some embodiments, see Figure 3 As shown, the above-mentioned adjustment of the excitation parameters of the target IDT according to the characteristic information data set of the target IDT may include the following contents during specific implementation:

[0144] S1: Determine a matching target processing rule according to the device identification of the target IDT;

[0145] S2: determining the problem factors causing the target IDT to fail to meet the operation requirements according to the target processing rule and the characteristic information data group of the target IDT;

[0146] S3: Determine a matching target adjustment strategy based on the problem factors, the operating rules of the target IDT and the sound field type;

[0147] S4: adjusting the excitation parameters of the target IDT according to the target adjustment strategy.

[0148] In specific implementation, the matching target processing rule can be determined by querying the preset processing rule set according to the device identification of the target IDT. The preset processing rule set may store multiple preset processing rules corresponding to multiple device identifications, and each preset processing rule may include the corresponding relationship between the problem factor and the characteristic information of the corresponding IDT.

[0149] Before specific implementation, a large number of test records for different interdigital transducers can be obtained in advance; then, for the test records of each interdigital transducer, clustering processing based on different problem factors is performed using a machine learning model to obtain the common relationship of key feature information for different problem factors of each interdigital transducer; then, based on the common relationship of key feature information for different problem factors of each interdigital transducer, a plurality of preset processing rules for different interdigital transducers are constructed; and a plurality of preset processing rules are combined to construct the preset processing rule set.

[0150] Among them, the above-mentioned problem factors may include one or more of the following: failure of the IDT, failure of other equipment associated with the IDT (for example, failure of the excitation module, etc.), errors in the preparation process of the IDT, differences between the cells targeted by the IDT and the expected cells, etc.

[0151] Specifically, for example, for the third interdigital transducer, based on the target processing rules and the characteristic information data group of the target interdigital transducer, when it is detected that the power transmission efficiency is less than the preset transmission efficiency threshold, it can be determined that there is an abnormality in the power amplifier circuit in the excitation module, and then the problem factor can be determined to be an excitation module failure associated with the third interdigital transducer.

[0152] For another example, for the first interdigital transducer, according to the target processing rules and the characteristic information data group of the target interdigital transducer, when it is detected that the temperature of the mixed cell is greater than the upper temperature limit of the first interdigital transducer, and the output power of the first interdigital transducer is less than the power bearing value of the first interdigital transducer, it can be determined that the first interdigital transducer is abnormal, and further it can be determined that the problem factor is a failure of the first interdigital transducer.

[0153] In specific implementation, different IDTs can be distinguished according to the target processing rules, and the characteristic information data of the target IDT can be used to determine the problem factors that cause the target IDT to fail to meet the operating requirements. Furthermore, different IDTs can be distinguished according to the problem factors, the operating rules of the target IDT, and the sound field type of the sound field provided by the target IDT, and a target characteristic strategy for the problem factors of the target IDT can be generated. Then, according to the target adjustment strategy, the excitation parameters of the target IDT are adjusted in a targeted manner so that the target IDT meets the operating requirements, thereby enabling the cell sorting system as a whole to obtain a better cell sorting effect.

[0154] The excitation parameters to be adjusted may be different types of excitation parameters corresponding to different IDTs and different problem factors. The excitation parameters may specifically include one or more of the following: transmission frequency, transmission width (or pulse width), transmission interval, transmission power, transmission phase, etc.

[0155] In some embodiments, considering that when operating requirements are not met, for different interdigital transducers, there are differences in the types of sound fields provided, the functional roles played, and the related problem factors, the main excitation parameters adjusted during adjustment may also be different.

[0156] Specifically, for example, for the first interdigital transducer, when the problem factor is the error in the preparation process of the interdigital transducer, combined with the type of sound field provided by the first interdigital transducer and the functional role it plays, the target adjustment strategy can be used to jointly adjust the transmission power and transmission phase of the first interdigital transducer in a targeted manner to quickly make the first interdigital transducer meet the operating requirements.

[0157] For another example, for the second interdigital transducer, when the problem factor is the error in the preparation process of the interdigital transducer, combined with the type of sound field provided by the second interdigital transducer and the functional role it plays, the target adjustment strategy can be used to jointly adjust the transmission power, transmission phase and transmission width of the second interdigital transducer in a targeted manner to quickly make the second interdigital transducer meet the operating requirements.

[0158] For another example, for the third interdigital transducer, when the problem factor is the error in the preparation process of the interdigital transducer, combined with the type of sound field provided by the third interdigital transducer and the functional role it plays, the target adjustment strategy can be adopted to jointly adjust the transmission power and transmission width of the third interdigital transducer in a targeted manner to quickly make the third interdigital transducer meet the operating requirements.

[0159] Based on the above embodiments, different types of IDTs can be effectively distinguished, and the relevant excitation parameters can be accurately adjusted according to specific problem factors and the operating rules and sound field types of the IDTs so that the IDTs can quickly meet the operating requirements.

[0160] In some embodiments, in addition to adjusting the excitation parameters of the target IDT, other adjustments may be made to the target IDT in response to different problem factors.

[0161] For example, in the case where the problem factor is a target IDT failure, a replacement prompt for the target IDT may also be generated to prompt the user to replace the target IDT in a timely manner.

[0162] In some embodiments, before acquiring characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells, referring to Figure 4 As shown, when the method is implemented specifically, it may also include the following contents:

[0163] S1: Start the cell sorting system and use the cell sorting system to sort and process the test cells;

[0164] S2: Acquiring test information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing test cells;

[0165] S3: According to the test rules, using the test information, perform an initial test on the cell sorting system to obtain corresponding test results;

[0166] S4: Based on the test results, if it is determined that at least one IDT in the cell sorting system does not meet the operation requirements, perform corresponding initial adjustments on the IDT.

[0167] The above-mentioned test cells can be specifically understood as reference cells whose cell types are known and whose cell states are healthy. Accordingly, the above-mentioned test rules can be test rules corresponding to the above-mentioned test cells. The above-mentioned test rules can include reference images corresponding to the test cells. The above-mentioned test rules can be specifically obtained by sorting out the test experiment data after a large number of test experiments are carried out on the test cells using a cell sorting system in advance.

[0168] In specific implementation, before using the cell sorting system to sort and process target cells, the cell sorting system can be started and used to perform an initial test on the interdigital transducers in the cell sorting system by sorting and processing test cells.

[0169] In a specific implementation, the image information, power information, and temperature related to each IDT can be collected separately; then, according to the test rules, it is first detected whether the output power of each IDT is greater than the power tolerance value of the IDT; at the same time, it is detected whether the cell temperature is greater than the upper limit value of the cell temperature tolerance (for example, the preset first temperature threshold).

[0170] When it is determined that the output power is greater than the power tolerance value of the interdigital transducer, and / or the cell temperature is greater than the upper limit of the cell temperature tolerance, it can be determined that the initial test has failed, and the user is prompted to check and adjust the relevant modules of the cell sorting system before using the cell sorting system to sort and process the target cells.

[0171] When it is determined that there is no output power less than the power tolerance value of the interdigital transducer and no cell temperature greater than the upper limit of the cell temperature tolerance, the matching degree between the calculated image information and the corresponding reference image is then detected to see whether the matching degree is greater than a preset matching degree threshold.

[0172] When it is determined that the matching degree is less than or equal to the preset matching degree threshold, it can be determined that the initial test has failed; and the user is prompted to check and adjust the relevant modules of the cell sorting system before using the cell sorting system to sort and process the target cells.

[0173] On the contrary, when it is determined that the matching degree is greater than the preset matching threshold, it can be determined that the initial test has passed; and the user is prompted to use the cell sorting system to sort and process the target cells.

[0174] Based on the above embodiments, it is possible to first screen and discover more obvious and important abnormal problems in the cell sorting system through a relatively efficient initial test according to the test rules, and promptly prompt the user to check and adjust the cell sorting system before sorting the target cells. In this way, on the one hand, it can effectively avoid the inactivation of the target cells due to some reasons caused by directly using the cell sorting system to sort and process the target cells, resulting in the loss of cell materials; on the other hand, it can effectively reduce the workload of subsequent detection and adjustment of each interdigital transducer in the cell sorting system.

[0175] In some embodiments, after adjusting the excitation parameters of the target IDT according to the characteristic information data group of the target IDT, the method may also include: binding the adjusted excitation parameters of the target IDT with the device identifier of the target IDT and the cell identifier of the target cell (such as cell type or cell state) to obtain a binding relationship corresponding to the cell of the target cell; and storing the binding relationship.

[0176] In this way, when the subsequent cell sorting system sorts and processes the target cells again, it can query and download the binding relationship according to the cell identifier of the target cells; according to the binding relationship, it obtains and deteriorates the excitation parameters according to the device identifier of the target interdigital transducer, adjusts the target interdigital transducer, and then sorts and processes the target cells to obtain better cell sorting effects.

[0177] As can be seen from the above, based on the cell sorting system and the adjustment method of the interdigital transducer provided in the embodiment of this specification, before the specific implementation, the cell sorting system can be improved first: the characteristic information acquisition unit is respectively set at the corresponding positions of the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer; at the same time, a feedback module and a control module are also introduced, and the feedback module is respectively connected to the characteristic information acquisition unit and the control module, and the control module is connected to the excitation module acting on the interdigital transducer. In the specific implementation, in the process of sorting and processing target cells based on the above-mentioned cell sorting system, the characteristic information about the interdigital transducer is first obtained; wherein the above-mentioned characteristic information at least carries the device identification of the corresponding interdigital transducer; according to the device identification, the characteristic information is divided into a plurality of characteristic information data groups corresponding to different interdigital transducers; at the same time, according to the device identification, the corresponding operation rules of the interdigital transducer and the type of the sound field are determined; then according to the operation rules and the type of the sound field of the interdigital transducer, by processing the corresponding characteristic information data group, it is automatically detected and determined whether the interdigital transducer in the cell sorting system meets the operation requirements. When it is determined that there is an IDT in the cell sorting system that does not meet the operating requirements, it is also possible to adjust the excitation parameters of the target IDT in a targeted manner based on the characteristic information of the target IDT that does not meet the operating requirements, so that the IDT in the cell sorting system can operate accurately according to the expected operating requirements, obtain better cell sorting effects, and effectively reduce damage to cells in related processes.

[0178] See also Figure 5 As shown, this specification also provides an excitation system for an IDT, which may at least include: a control module, an IDT, a feedback module, an excitation module, etc.; wherein the excitation module may be connected to the IDT;

[0179] The feedback module is used to collect characteristic information related to the interdigital transducer;

[0180] The control module is used to detect whether the IDT meets the operation requirements according to the characteristic information; and if it is determined that the IDT does not meet the operation requirements, adjust the excitation parameters of the IDT through the excitation module.

[0181] Specifically, the above control module may include an FPGA main control module.

[0182] The excitation system of the IDT may further include a power supply module for providing the required electrical energy to the excitation system of the IDT.

[0183] The above-mentioned IDT excitation system may further include an interaction module for receiving user-defined information input so that the subsequent control module can perform diversified detection and adjustment on the relevant IDT in combination with the user-defined information.

[0184] Specifically, the IDT may include one IDT or a plurality of IDTs, wherein the plurality of IDTs may be IDTs of the same type or of different types.

[0185] Specifically, the feedback module may acquire characteristic information related to the IDT through a corresponding characteristic information acquisition unit.

[0186] Specifically, when the control module determines that the IDT does not meet the operating requirements, it can distinguish different IDTs, and adopt a matching method for the IDT that does not meet the operating requirements (which can be recorded as the target IDT), and make targeted adjustments to the excitation parameters of the IDT through the excitation module based on the characteristic information of the IDT, so that the IDT meets the operating requirements.

[0187] In specific implementation, based on the above-mentioned IDT excitation system, when the relevant IDT is used for operation, the characteristic information about the IDT can be first collected by using feedback; wherein the characteristic information about the IDT includes: characteristic information of the IDT itself and / or characteristic information of the object (or object of sound wave action, such as cells, etc.) targeted by the IDT. The characteristic information can specifically include one or more of the following: temperature information, power information, image information, etc.

[0188] The control module can distinguish different IDTs based on the characteristic information collected by the feedback module, and detect whether the IDT currently in operation meets the operation requirements. In the case where it is determined that the IDT does not meet the operation requirements, the excitation parameters of the IDT can be adjusted in a targeted manner through the excitation module based on the characteristic information about the IDT, so that the IDT meets the operation requirements, thereby obtaining a better application effect. The excitation parameters include one or more of the following: transmission frequency, transmission width, transmission interval, transmission power, transmission phase, etc.

[0189] Based on the above method, the optimal excitation parameters of the IDT excitation system for the object can be determined. Furthermore, the device identification of the relevant IDT, the optimal excitation parameters, and the object identification of the object can be bound and stored so that they can be directly retrieved and used later.

[0190] Specifically, for example, the device identification, optimal excitation parameters, and object identification of the above-mentioned related IDT can be bound to obtain a corresponding binding relationship; then an identification code (for example, a QR code) corresponding to the binding relationship can be generated; and the identification code can be associated with the object. In this way, when the subsequent user uses the above-mentioned IDT excitation system to process the object, he only needs to scan the identification code to automatically query and determine the relevant optimal excitation parameters based on the binding relationship; then, according to the optimal excitation parameters, the related IDT is adjusted, and the adjusted IDT is used to process the object to obtain a better processing effect.

[0191] This specification embodiment provides an electronic device, referring to Figure 6 The electronic device includes a network communication port 601, a processor 602 and a memory 603, and the above structures are connected through internal cables so that each structure can perform specific data interaction.

[0192] The network communication port 601 can be specifically used to receive a trigger instruction.

[0193] The processor 602 can be specifically used to respond to a trigger instruction and obtain characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells; wherein the characteristic information at least carries the device identification of the corresponding interdigital transducer; according to the device identification, the characteristic information is divided into multiple characteristic information data groups; wherein, one characteristic information data group corresponds to one interdigital transducer; according to the device identification, the operation rule of the corresponding interdigital transducer and the sound field type of the sound field provided by the interdigital transducer are determined; according to the operation rule and the sound field type of the interdigital transducer, by processing the corresponding characteristic information data group, it is detected whether the interdigital transducer in the cell sorting system meets the operation requirements; when it is determined that there is an interdigital transducer that does not meet the operation requirements, the interdigital transducer that does not meet the operation requirements is determined as the target interdigital transducer; according to the characteristic information data group of the target interdigital transducer, the excitation parameters of the target interdigital transducer are adjusted.

[0194] The memory 603 may be specifically used to store corresponding instruction programs, as well as characteristic information, characteristic information data, excitation parameters and other related data about the IDT.

[0195] Based on the above method, the relevant structural performance of the electronic device can be effectively utilized, the data processing speed of the electronic device can be improved, and the data processing of the adjustment of the interdigital transducer can be efficiently realized.

[0196] In this embodiment, the network communication port 601 can be a virtual port that is bound to different communication protocols so that different data can be sent or received. For example, the network communication port can be a port responsible for web data communication, a port responsible for FTP data communication, or a port responsible for email data communication. In addition, the network communication port can also be a physical communication interface or communication chip. For example, it can be a wireless mobile network communication chip, such as GSM, CDMA, etc.; it can also be a Wifi chip; it can also be a Bluetooth chip.

[0197] In this embodiment, the processor 602 may be implemented in any appropriate manner. For example, the processor may take the form of a microprocessor or processor and a computer-readable medium storing a computer-readable program code (such as software or firmware) executable by the (micro)processor, a logic gate, a switch, an application specific integrated circuit (ASIC), a programmable logic controller, and an embedded microcontroller, etc. This specification does not limit this.

[0198] In this embodiment, the memory 603 may include multiple levels. In a digital system, anything that can store binary data can be a memory; in an integrated circuit, a circuit with a storage function but no physical form is also called a memory, such as RAM, FIFO, etc.; in a system, a storage device with a physical form is also called a memory, such as a memory stick, TF card, etc.

[0199] The embodiment of the present specification also provides a computer-readable storage medium based on the above-mentioned IDT adjustment method, wherein the computer-readable storage medium stores computer program instructions, and when the computer program instructions are executed, the following are achieved: obtaining characteristic information about the IDT collected by the cell sorting system during the process of sorting and processing target cells; wherein the characteristic information at least carries the device identification of the corresponding IDT; according to the device identification, the characteristic information is divided into multiple characteristic information data groups; wherein one characteristic information data group corresponds to one IDT respectively; according to the device identification, determining the operation rule of the corresponding IDT and the sound field type of the sound field provided by the IDT; according to the operation rule and the sound field type of the IDT, by processing the corresponding characteristic information data group, detecting whether the IDT in the cell sorting system meets the operation requirements; when it is determined that there is an IDT that does not meet the operation requirements, determining the IDT that does not meet the operation requirements as the target IDT; and adjusting the excitation parameters of the target IDT according to the characteristic information data group of the target IDT.

[0200] In this embodiment, the storage medium includes, but is not limited to, a random access memory (RAM), a read-only memory (ROM), a cache, a hard disk (HDD), or a memory card. The memory may be used to store computer program instructions. The network communication unit may be an interface for network connection communication set in accordance with the standard specified by the communication protocol.

[0201] In this embodiment, the functions and effects specifically implemented by the program instructions stored in the computer-readable storage medium can be explained in comparison with other implementations and will not be described in detail here.

[0202] The embodiments of the present specification also provide a computer program product, which at least includes a computer program, and when the computer program is executed by a processor, the following method steps are implemented: obtaining characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells; wherein the characteristic information at least carries the device identification of the corresponding interdigital transducer; according to the device identification, the characteristic information is divided into multiple characteristic information data groups; wherein one characteristic information data group corresponds to one interdigital transducer; according to the device identification, the operation rule of the corresponding interdigital transducer and the sound field type of the sound field provided by the interdigital transducer are determined; according to the operation rule and the sound field type of the interdigital transducer, by processing the corresponding characteristic information data group, whether the interdigital transducer in the cell sorting system meets the operation requirements; when it is determined that there is an interdigital transducer that does not meet the operation requirements, the interdigital transducer that does not meet the operation requirements is determined as the target interdigital transducer; according to the characteristic information data group of the target interdigital transducer, the excitation parameters of the target interdigital transducer are adjusted.

[0203] See also Figure 7 As shown, the embodiment of this specification also provides an adjustment device for an interdigital transducer, which may specifically include the following structural modules:

[0204] The acquisition module 701 may be specifically used to acquire characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells; wherein the characteristic information at least carries a device identification of the corresponding interdigital transducer;

[0205] The division module 702 may be specifically configured to divide the characteristic information into a plurality of characteristic information data groups according to the device identification; wherein each characteristic information data group corresponds to one interdigital transducer;

[0206] The first determination module 703 may be specifically used to determine the operation rule of the corresponding IDT and the sound field type of the sound field provided by the IDT according to the device identification;

[0207] The detection module 704 may be specifically used to detect whether the interdigital transducer in the cell sorting system meets the operation requirements by processing the corresponding characteristic information data group according to the operation rules and acoustic field type of the interdigital transducer;

[0208] The second determination module 705 may be specifically configured to, when it is determined that there is an IDT that does not meet the operation requirement, determine the IDT that does not meet the operation requirement as a target IDT;

[0209] The adjustment module 706 may be specifically configured to adjust the excitation parameters of the target IDT according to the characteristic information data set of the target IDT.

[0210] In some embodiments, the characteristic information may specifically include at least one of the following: temperature information, image information, power information, and the like.

[0211] In some embodiments, when the above-mentioned adjustment module 706 is implemented, the excitation parameters of the target IDT can be adjusted according to the characteristic information data group of the target IDT in the following manner: according to the device identification of the target IDT, a matching target processing rule is determined; according to the target processing rule and the characteristic information data group of the target IDT, the problem factors that cause the target IDT to fail to meet the operating requirements are determined; according to the problem factors, the operating rules and the sound field type of the target IDT, a matching target adjustment strategy is determined; according to the target adjustment strategy, the excitation parameters of the target IDT are adjusted.

[0212] In some embodiments, before obtaining characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells, the device, when implemented, can also be used to: start the cell sorting system, and use the cell sorting system to sort and process test cells; obtain test information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing test cells; according to the test rules, use the test information to perform an initial test on the cell sorting system to obtain corresponding test results; according to the test results, when it is determined that at least one interdigital transducer in the cell sorting system does not meet the operating requirements, perform corresponding initial adjustments to the interdigital transducer.

[0213] It should be noted that the units, devices or modules described in the above embodiments can be implemented by computer chips or entities, or by products with certain functions. For the convenience of description, the above devices are described separately by functions divided into various modules. Of course, when implementing this specification, the functions of each module can be implemented in the same or more software and / or hardware, or the modules that implement the same function can be implemented by a combination of multiple sub-modules or sub-units. The device embodiments described above are only schematic. For example, the division of the units is only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, indirect coupling or communication connection of devices or units, which can be electrical, mechanical or other forms.

[0214] As can be seen from the above, the adjustment device of the IDT provided in the embodiment of this specification can automatically detect and determine whether the IDT in the cell sorting system meets the operating requirements. In the case of determining that there is an IDT in the cell sorting system that does not meet the operating requirements, it is also possible to adjust the excitation parameters of the target IDT in a targeted manner based on the characteristic information of the target IDT that does not meet the operating requirements, so that the IDT in the cell sorting system can accurately operate according to the expected operating requirements, obtain a better cell sorting effect, and effectively reduce the damage to the cells in the related process.

[0215] In a specific scenario example, the cell sorting system, IDT adjustment method and device-related ideas provided in this specification can also be applied to construct an IDT excitation system that automatically adjusts excitation parameters. The specific implementation process can be found in the following content.

[0216] In this scenario example, considering that when multiple groups of interdigital transducers are involved in the system, the transducers with the same design requirements cannot ensure good consistency due to the processing technology, resulting in certain differences in the performance parameters of different interdigital transducers of the same type. Relatively speaking, the performance parameters of transducers with different design requirements (for example, different types of interdigital transducers) are more different. Each group of transducers needs to test and select excitation parameters to achieve the expected excitation performance and output the required sound waves. And each group of transducers will also have different sound wave action objects (for example, different types of cells), and it is necessary to select the best excitation parameters for different objects so that the transducer has the best effect (for example, deformation amount) on the sound wave action object.

[0217] In view of the above situation, it is considered that the parameters can be automatically adjusted through a feedback loop (for example, a feedback module and a control module) according to different IDTs and sound wave action objects to test and select the best excitation parameters; and the parameters can be recorded and saved for direct call next time.

[0218] Specifically, it is considered that: first, the frequency response of the IDT refers to the response capability at different frequencies. In practical applications, frequency response is one of the most important parameters of the IDT. The adverse effects of frequency response are mainly manifested in two aspects: one is that the frequency band is not wide enough, and the other is that the frequency response is not smooth. If the frequency band is not wide enough, the IDT cannot work correctly, and the frequency response is not smooth, which will cause signal distortion, thereby affecting the performance of the IDT. Secondly, the gain of the IDT refers to the proportional relationship between the input signal and the output signal. The gain is related to the strength of the signal. The greater the signal strength, the greater the gain. The gain of the IDT has a direct impact on the quality of the signal. If the gain value is too small, part of the signal will be lost during the signal processing process, resulting in a decrease in signal quality; if the gain value is too large, over-amplification will occur during the signal processing process, resulting in signal distortion. Therefore, in practical applications, it is necessary to select an appropriate gain value according to specific signal conditions. Furthermore, the dynamic range of the IDT refers to the ratio of the maximum signal amplitude to the minimum signal amplitude that the IDT can process. The larger the dynamic range, the wider the signal range that can be processed. In practical applications, if the dynamic range is too small, the high-amplitude signal will be masked by the background noise, affecting the sensor's detection ability. Then, the linearity of the interdigital transducer refers to the degree of linear relationship between the output signal and the input signal. The interdigital transducer requires high linearity under certain application conditions, because high linearity can make the sensor's measurement results more accurate and reduce measurement errors.

[0219] Based on the above considerations, it can be seen that the performance of the interdigital transducer is affected by many parameters, including frequency response, gain, dynamic range and linearity. In practical applications, it is necessary to select and optimize according to the specific application situation to achieve the best performance state.

[0220] Furthermore, when a system involves multiple groups of interdigital transducers, each group of transducers may have the same design requirements but the existing processing technology cannot guarantee good consistency, so they have different performance parameters such as frequency response, gain, dynamic range and linearity; or they have different design requirements (operating frequency and bandwidth, output power, working time, etc.), so they have different physical parameters such as structure, size, material, etc., and the performance parameters will be more different. For each group of transducers, it is necessary to apply different excitation parameters according to the performance of the transducer, including waveform, frequency, amplitude, duty cycle, etc., so that the interdigital transducer can achieve the expected performance state. Among them, each group of transducers has multiple usage requirements or usage scenarios, and there will be different sound wave action objects. Different excitation parameters need to be applied to different sound wave action objects, including amplitude, phase, duty cycle, etc., in order to control the interdigital transducer to achieve the best effect on the sound wave action object.

[0221] However, existing methods and devices generally only provide an interface for selecting excitation parameters, but have no feedback loop, and cannot automatically adjust the excitation parameters according to feedback information to obtain the best acoustic wave effect. For different IDTs and different acoustic wave action objects, it is impossible to quickly obtain the best excitation parameters.

[0222] In response to the above problems, in this scenario example, it is proposed to introduce and use a feedback loop to automatically adjust the excitation parameters according to the effect of the transducer on different acoustic wave action objects, and record and save the corresponding excitation parameters of the corresponding transducer on a certain acoustic wave action object under the best acoustic wave effect. Under the same requirements, when this group of transducers is stimulated again, it is only necessary to call the saved excitation parameters.

[0223] For details, see Figure 8 As shown, the interdigital transducer excitation system for automatically adjusting the excitation parameters provided in this scenario example is composed of five parts. Among them, the FPGA main control system (for example, the control module) is responsible for the control of the system, such as the generation and control of the excitation signal, the calculation and processing of the feedback loop data, the processing of the human-computer interaction process, and the adjustment and preservation of the parameters. The power module provides the required power for the system. The human-computer interaction (for example, the interaction module) provides an interface that the user can operate, and scans the identification code of the interdigital transducer to retrieve the saved excitation parameters. The excitation module provides the generation of the interdigital transducer excitation signal and the amplification of the power. The feedback module includes the detection of power (a type of power information), the collection of the probe temperature (a type of temperature information), and the collection of the image of the object acted by the sound wave (a type of image information).

[0224] Specifically, the above incentive module can be found in Fig. 9 As shown, the above feedback module can be found in Fig.10 shown.

[0225] Specifically, by introducing a feedback module, the excitation parameters of the interdigital transducer are fine-tuned, including but not limited to the transmission frequency, transmission width, transmission interval, transmission power, and transmission phase, so as to achieve the best excitation performance and acoustic wave effect. The specific implementation may include the following contents.

[0226] First, the power detection module can provide feedback data to monitor the forward power and reverse power of the IDT driven by the power amplifier circuit, so as to evaluate the power transmission efficiency of the excitation module to the transducer.

[0227] Second, the temperature acquisition module can provide feedback data to monitor the temperature change of the IDT during operation, thereby evaluating whether the IDT is in a normal working state.

[0228] Third, the image acquisition and processing unit can provide feedback data to monitor the deformation of the object (such as cells) acted upon by the acoustic wave, thereby evaluating the effect of the interdigital transducer on the object acted upon by the acoustic wave.

[0229] Based on the above feedback data, the excitation efficiency of the excitation module on the IDT and the effect on the object of acoustic wave action under a certain set of excitation parameters can be comprehensively evaluated. According to the evaluation data, the excitation parameters are automatically adjusted until the optimal acoustic wave effect of the IDT is achieved under normal working conditions.

[0230] After obtaining the optimal excitation parameters of the IDT, you can scan the one-dimensional code of the transducer and the object of the sound wave action, and bind the two one-dimensional codes with the parameters and store them on the PC device. When used later, scan the one-dimensional code of the transducer and the object of the sound wave action to call out the relevant parameters and send them with one click.

[0231] Based on the above scenario examples, the cell sorting system, IDT adjustment method and device provided in this manual are verified. For different IDTs and their corresponding sound wave action objects, the excitation parameters of the excitation circuit are automatically adjusted through the information provided by the feedback loop, and the optimal parameters are recorded and saved, which can be directly called next time.

[0232] Although the present specification provides method operation steps as described in the embodiments or flow charts, more or less operation steps may be included based on conventional or non-creative means. The order of steps listed in the embodiments is only one way of executing the order of many steps, and does not represent a unique execution order. When the device or client product in practice is executed, it can be executed in sequence or in parallel according to the method shown in the embodiments or the drawings (for example, a parallel processor or a multi-threaded processing environment, or even a distributed data processing environment). The term "include", "include" or any other variant thereof is intended to cover non-exclusive inclusion, so that the process, method, product or device including a series of elements includes not only those elements, but also includes other elements that are not explicitly listed, or also includes elements inherent to such a process, method, product or device. In the absence of more restrictions, it is not excluded that there are other identical or equivalent elements in the process, method, product or device including the elements. The first, second, etc. words are used to represent the name, and do not represent any particular order.

[0233] Those skilled in the art also know that, in addition to implementing the controller in a purely computer-readable program code, the controller can be made to implement the same function in the form of logic gates, switches, application-specific integrated circuits, programmable logic controllers, and embedded microcontrollers by logically programming the method steps. Therefore, such a controller can be considered as a hardware component, and the devices for implementing various functions included therein can also be considered as structures within the hardware component. Or even, the devices for implementing various functions can be considered as both software modules for implementing the method and structures within the hardware component.

[0234] This specification may be described in the general context of computer-executable instructions executed by a computer, such as program modules. Generally, program modules include routines, programs, objects, components, data structures, classes, etc. that perform specific tasks or implement specific abstract data types. This specification may also be practiced in distributed computing environments where tasks are performed by remote processing devices connected through a communication network. In a distributed computing environment, program modules may be located in local and remote computer-readable storage media including storage devices.

[0235] Through the description of the above embodiments, it can be known that those skilled in the art can clearly understand that the present specification can be implemented by means of software plus a necessary general hardware platform. Based on such an understanding, the technical solution of the present specification can essentially be embodied in the form of a software product, which can be stored in a storage medium such as ROM / RAM, a disk, an optical disk, etc., and includes a number of instructions for enabling a computer device (which can be a personal computer, a mobile terminal, a server, or a network device, etc.) to execute the methods described in each embodiment of the present specification or some parts of the embodiments.

[0236] The various embodiments in this specification are described in a progressive manner, and the same or similar parts between the various embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. This specification can be used in many general or special computer system environments or configurations. For example: personal computers, server computers, handheld devices or portable devices, tablet devices, multi-processor systems, microprocessor-based systems, set-top boxes, programmable electronic devices, network PCs, minicomputers, mainframe computers, distributed computing environments including any of the above systems or devices, etc.

[0237] Although the present specification is described through embodiments, those skilled in the art will appreciate that there are many modifications and changes to the present specification without departing from the spirit of the present specification, and it is intended that the appended claims include these modifications and changes without departing from the spirit of the present specification.

Claims

1. A cell sorting system, characterized in that: At least includes: a cell transport pipeline, a feedback module, an excitation module, and a control module; wherein, The end of the cell transport pipeline is connected to at least a first sub-pipeline and a second sub-pipeline; and a cell arrangement module, a cell squeezing module, a cell positioning module, and a cell sorting module are sequentially arranged along the cell transport pipeline; The cell arrangement module includes at least a first interdigital transducer, the cell squeezing module includes at least a second interdigital transducer, and the cell sorting module includes at least a third interdigital transducer; wherein the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer are respectively connected to an excitation module; and the excitation module is connected to a control module; Matching feature information acquisition units are also respectively arranged at corresponding positions of the first interdigital transducer, the second interdigital transducer, and the third interdigital transducer; The characteristic information acquisition unit is connected to a feedback module, and the feedback module is used to acquire characteristic information about the interdigital transducer through the characteristic information acquisition unit; The control module is connected to the feedback module, and the feedback module is also used to send the characteristic information to the control module; The control module is used to detect and determine a target IDT that does not meet the operating requirements from the first IDT, the second IDT, and the third IDT according to the characteristic information; and adjust the excitation parameters of the target IDT through the excitation module.

2. The system according to claim 1, characterized in that The characteristic information acquisition unit includes at least one of the following: a microscopic camera, a temperature sensor, and a power detection circuit.

3. The system according to claim 1, characterized in that The third IDT includes a plurality of sub-IDTs.

4. A method for adjusting an interdigital transducer of a cell sorting system according to any one of claims 1 to 3, characterized in that: include: Acquiring characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells; wherein the characteristic information at least carries a device identification of the corresponding interdigital transducer; According to the device identification, the characteristic information is divided into a plurality of characteristic information data groups; wherein one characteristic information data group corresponds to one interdigital transducer; According to the device identification, the operation rule of the corresponding interdigital transducer and the sound field type of the sound field provided by the interdigital transducer are determined; According to the operation rules and acoustic field types of the IDT, by processing the corresponding characteristic information data group, detecting whether the IDT in the cell sorting system meets the operation requirements; In the case where it is determined that there is an IDT that does not meet the operation requirements, the IDT that does not meet the operation requirements is determined as a target IDT; According to the characteristic information data set of the target IDT, the excitation parameters of the target IDT are adjusted.

5. The method according to claim 4, characterized in that The characteristic information includes at least one of the following: temperature information, image information, and power information.

6. The method according to claim 4, characterized in that According to the characteristic information data set of the target IDT, adjusting the excitation parameters of the target IDT includes: According to the device identification of the target interdigital transducer, a matching target processing rule is determined; Determine the problem factors that cause the target interdigital transducer to fail to meet the operation requirements according to the target processing rules and the characteristic information data group of the target interdigital transducer; Determine the matching target adjustment strategy based on the problem factors, the operating rules of the target IDT and the sound field type; According to the target adjustment strategy, the excitation parameters of the target IDT are adjusted.

7. The method according to claim 4, characterized in that Before acquiring characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells, the method further includes: Starting a cell sorting system, and using the cell sorting system to sort and process test cells; Acquiring test information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing test cells; According to the test rules, using the test information, the cell sorting system is initially tested to obtain corresponding test results; According to the test results, when it is determined that at least one IDT in the cell sorting system does not meet the operation requirements, the IDT is initially adjusted accordingly.

8. An excitation system for an interdigital transducer, characterized in that: At least: A control module, an IDT, a feedback module, and an excitation module; wherein the excitation module is connected to the IDT; The feedback module is used to collect characteristic information related to the interdigital transducer; The control module is used to detect whether the IDT meets the operation requirements according to the characteristic information; and if it is determined that the IDT does not meet the operation requirements, adjust the excitation parameters of the IDT through the excitation module.

9. An adjustment device for an interdigital transducer, characterized in that: include: An acquisition module, used to acquire characteristic information about the interdigital transducer collected by the cell sorting system during the process of sorting and processing target cells; wherein the characteristic information at least carries a device identification of the corresponding interdigital transducer; A division module, used for dividing the characteristic information into a plurality of characteristic information data groups according to the device identification; wherein one characteristic information data group corresponds to one interdigital transducer respectively; A first determination module is used to determine the operation rule of the corresponding IDT and the sound field type of the sound field provided by the IDT according to the device identification; A detection module, used to detect whether the interdigital transducer in the cell sorting system meets the operation requirements by processing the corresponding characteristic information data group according to the operation rules and acoustic field type of the interdigital transducer; A second determination module is used to determine the IDT that does not meet the operation requirements as a target IDT when it is determined that there is an IDT that does not meet the operation requirements; The adjustment module is used to adjust the excitation parameters of the target IDT according to the characteristic information data group of the target IDT.

10. A computer-readable storage medium, characterized in that: Computer instructions are stored thereon, and when the instructions are executed by a processor, the steps of the method according to any one of claims 4 to 7 are implemented.

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