Liquid tank system capable of effectively monitoring condition of working liquid

Abstract

A liquid tank system capable of effectively monitoring working solution conditions comprises a liquid tank comprising a tank body and a tank cover, and the volume of the tank body is the volume of nutrient solution required by culture of at least one group of hollow fiber bioreactors; the weighing structure is used for weighing the weight of the liquid tank and the liquid in the tank body in real time to obtain an actual weight value; the control structure receives the actual weight value in real time and compares the actual weight value with a theoretical weight value, the theoretical weight value is the sum of the weight of the tank body and the weight of liquid needed by the hollow fiber bioreactor of the group number needed by culture and has a fluctuation value, and when the actual weight value is the difference value of the theoretical weight value and the fluctuation value, the control structure controls the input of a nutrient solution into the tank body; and when the actual weight value is the sum of the theoretical weight value and the fluctuation value, the control structure controls the liquid in the tank body to flow out. According to the arrangement, the liquid in the tank body can be well maintained within the fluctuation range of the theoretical weight value, and the relative balance state is maintained, so that the culture requirements of different groups of hollow fiber bioreactors are met.

Description

Technical Field

[0001] The present invention belongs to the technical field of cell culture equipment, and in particular to the technical field of culture equipment of hollow fiber bioreactors, and specifically is a liquid tank device capable of effectively monitoring the condition of a working fluid. Background Art

[0002] CN112755288B is an extracorporeal liver support system that is currently used temporarily by patients with liver failure or waiting for a liver source to replace the liver. The system uses a C3A hollow fiber bioreactor to play a liver replacement role. It is necessary to complete the cultivation of the hollow fiber bioreactor in advance.

[0003] At present, when hollow fiber bioreactors are used for cultivation, the growth rate and activity of cells in the hollow fiber bioreactor need to be guaranteed, and the nutrient composition, pH value, oxygen content, various ion contents, etc. of the nutrient solution entering the hollow fiber bioreactor need to be strictly controlled. Therefore, during cultivation, the nutrient solution cannot be directly connected to the hollow fiber bioreactor. Instead, an intermediate liquid tank for transitional working liquid is set. The liquid tank contains a fixed space, and the liquid in the liquid tank is ensured to meet the nutrient requirements of the hollow fiber bioreactor by continuously inputting new culture solution and extracting the liquid in the liquid tank.

[0004] The volume of the existing liquid tank for hollow fiber bioreactors is deterministic and is used to culture one group of four bioreactors. The volume is fixed and can only be used to culture one group of four bioreactors. However, as the demand changes, the number of bioreactors to be cultured often changes, and the liquid tank with a fixed volume will not be able to meet the requirements of multiple groups of culture. However, in the specific operation process, the volume in the tank body also needs to correspond to the liquid volume required for all the hollow fiber bioreactors to be cultured, and it is obviously unscientific to repeatedly replace liquid tanks of different volumes. Therefore, a liquid tank device is needed to effectively monitor the amount of liquid in the intermediate tank according to the needs and correspond to the working liquid required by the hollow fiber bioreactor.

[0005] In view of the above technical problems, the present invention provides a liquid tank device which can effectively monitor the condition of working fluid. Summary of the invention

[0006] The liquid tank in the prior art is a liquid tank with a fixed volume, and the amount of working liquid in the liquid tank is the same as the amount of working liquid in the hollow fiber bioreactor. During the process, it is only necessary to initially monitor whether the liquid tank is full, and then the working liquid amount in the liquid tank is ensured by controlling the input speed of the external nutrient liquid into the liquid tank and balancing the amount of working liquid output by the liquid tank waste liquid system and by pressure surface monitoring. However, if the above method is applied to the cultivation of multiple groups of bioreactors, the number of groups of hollow fiber bioreactors cultivated each time is not fixed, and the cultivation is more based on demand. This is difficult to complete through liquid level monitoring. Therefore, how to set the volume of the liquid tank to meet the cultivation of multiple groups of hollow fiber bioreactors and how to set the appropriate working liquid amount according to the number of groups of hollow fiber bioreactors during the process and monitor it in real time during the process are key issues in completing multiple groups of hollow fiber bioreactors.

[0007] The present invention sets the volume of the liquid tank according to the situation of culturing the most groups of hollow fiber bioreactors each time, and calculates the amount of working liquid required during the culturing process of each group of hollow fiber bioreactors, so that the amount of working liquid in the liquid tank corresponds to the amount of working liquid for the corresponding number of groups, and maintains the state of liquid in and out of the liquid tank by monitoring the mass of the liquid tank. This method of monitoring the mass of the liquid tank to maintain the balance of liquid in and out of the liquid tank can also be used to monitor the incoming liquid of a single group of hollow fiber bioreactors.

[0008] The specific technical solution is: a liquid tank system that can effectively monitor the working fluid condition, which includes:

[0009] The liquid tank comprises a tank body and a tank cover. Various openings are arranged on the tank cover for arranging various connectors. The volume of the tank body is sufficient to meet the volume of the nutrient solution required for culturing at least one group of hollow fiber bioreactors.

[0010] A weighing structure is used to weigh the liquid tank and the liquid in the tank in real time to obtain the actual weight value;

[0011] The control structure receives the actual weight value of the weighing structure in real time and compares it with the theoretical weight value. The theoretical weight value is the sum of the tank weight and the liquid weight required for culturing the required number of hollow fiber bioreactors, and there is a fluctuation value of the theoretical weight. When the actual weight value is the difference between the theoretical weight value and the fluctuation value, the control structure controls the input of nutrient solution into the tank. When the actual weight value is the sum of the theoretical weight value and the fluctuation value, the control structure controls the liquid out of the tank, and the process repeats itself.

[0012] The above arrangement can well maintain the liquid in the tank within the threshold range of the theoretical weight value and maintain a relative equilibrium state, but the liquid will not mix at the same time. An effective buffer stage is provided for liquid mixing to avoid liquid waste caused by improper liquid mixing. In addition, the cultivation needs of different groups of hollow fiber bioreactors can be met.

[0013] Furthermore, the range of the fluctuation value is 50-100 g, which can meet the needs of culture medium fluctuation and will not cause any impact.

[0014] Furthermore, the tank cover of the liquid tank is provided with a nutrient solution inlet joint, which is used to introduce the nutrient solution in the nutrient solution delivery structure into the tank body, and a nutrient solution outlet joint, which is used to lead the nutrient solution from the tank body to the waste liquid collection structure; a reactor inlet joint, which is used to transport the working liquid in the tank body to the hollow fiber bioreactor; a reactor outlet joint, which is used to introduce the liquid in the hollow fiber bioreactor into the tank body; wherein the reactor inlet flow rate is the same as the reactor outlet flow rate; two liquid passages are constructed by the setting of the above joints, one is the liquid passage 1 between the liquid tank and the nutrient solution delivery structure and the waste liquid collection structure; the other is the liquid passage 2 for the circulation of the liquid in the hollow fiber bioreactor and the liquid tank; through these two liquid passages, effective cultivation of the hollow fiber bioreactor is achieved, wherein the liquid passage 1 realizes the replacement of the liquid in the tank body, and the liquid passage 2 directly provides nutrients for the hollow fiber bioreactor. Wherein, both the liquid passage 1 and the liquid passage 2 are provided with power pumps, wherein the pump of the liquid passage 2 is a gear pump, which replaces the original magnetic pump. Through this setting, the liquid filling process during the calibration process can be omitted.

[0015] Furthermore, because real-time weighing is carried out throughout the entire process, in order to ensure the stability of the overall structure during weighing, a support frame is set outside the liquid tank, and inclined support feet are set at the bottom of the support frame. By setting the inclined support feet, the support area can be increased to increase the stability of the liquid tank when it is placed. During weighing, the weight of the support frame is also within the weighing range as part of the initial weight of the liquid tank.

[0016] Furthermore, the support frame includes a fixing ring arranged on the top, the inner diameter of the fixing ring is smaller than the outer diameter of the edge of the tank body, and the diameter of the fixing ring is equal to the outer diameter of the tank cover, and a sealing ring is also arranged on the tank body; the fixing ring and the tank cover are fixed together, and the sealing ring realizes the sealing of the tank cover and the tank body; in this way, the support frame and the tank body are stably combined together to form a whole that can be weighed together, and in this way, the stability of the liquid tank when placed can be achieved to the greatest extent.

[0017] Furthermore, the support frame includes multiple support rods, an inclined rod-shaped support foot is arranged under each support rod, a fixing ring is arranged above the support rod, and a connecting ring fixed to the inner side of the multiple support rods is arranged under the support rod. The upper fixing ring and the lower connecting ring are combined with the support rod to construct a stable support frame.

[0018] Furthermore, the weighing structure includes a receiving plate for receiving the liquid tank. The receiving plate is configured as a circular plate. A central area and a recessed ring on the periphery of the central area which is lower than the central area are provided on the circular plate to receive the support legs of the support frame. A blocking ring perpendicular to the recessed ring is also provided on the outside of the recessed ring. The support legs are arranged in the recessed ring and the bottom of the tank body is arranged in the central area. In this way, the liquid tank can be well fixed on the receiving plate.

[0019] Furthermore, a containing structure for accommodating the weighing structure is provided, a cavity is provided in the containing structure, the bearing structure is provided in the cavity, and a circular hole consistent with the outer diameter of the blocking ring is provided on the containing structure. Through this arrangement, the weighing structure can be uniformly fixed or movably provided to the bottom of the incubator.

[0020] Furthermore, in order to ensure the operation of the entire liquid tank when cleaning the tank body and the tank cover, the liquid tank is fixed to the edge of the bottom of the incubator, and the liquid tank is set as a movable tank body that can be moved out of the incubator. The containment structure is set as a structure that can be translated; a track groove equal to the width of the containment structure is set at the bottom of the incubator, the track groove is the groove body in the middle of the track structure, and track 1 is set on the side walls of the track structure. Correspondingly, track 2 adapted to track 1 is set on the containment structure. The containment structure is moved out of the incubator with the liquid tank by relative movement of track 1 relative to track 2.

[0021] The present invention also discloses a stably placed liquid tank, which includes a liquid tank and a support frame arranged on the outside of the liquid tank. Inclined supporting feet are arranged at the bottom of the support frame. By arranging the inclined supporting feet, the stability of the liquid tank during placement can be increased in a manner of increasing the supporting area. When weighing, the weight of the support frame is also within the weighing range as a part of the initial weight of the liquid tank.

[0022] Furthermore, the support frame includes a fixing ring arranged on the top, the inner diameter of the fixing ring is smaller than the outer diameter of the edge of the tank body, and the diameter of the fixing ring is equal to the outer diameter of the tank cover, and a sealing ring is also arranged on the tank body; the fixing ring and the tank cover are fixed together, and the sealing ring realizes the sealing of the tank cover and the tank body; in this way, the support frame and the tank body are stably combined together to form a whole that can be weighed together, and in this way, the stability of the liquid tank when placed can be achieved to the greatest extent.

[0023] Furthermore, the support frame includes multiple support rods, an inclined rod-shaped support foot is arranged under each support rod, a fixing ring is arranged above the support rod, and a connecting ring fixed to the inner side of the multiple support rods is arranged under the support rod. The upper fixing ring and the lower connecting ring are combined with the support rod to construct a stable support frame.

[0024] The present invention also discloses a weighing structure for weighing a liquid tank, which includes a weighing module and a receiving plate. The receiving plate is arranged on the weighing module, and the receiving plate is arranged as a circular plate. A central area and a recessed ring on the periphery of the central area which is lower than the central area are arranged on the circular plate, and are used to receive the support feet of the support frame, and a blocking ring perpendicular to the recessed ring is also arranged on the outside of the recessed ring; the support feet are arranged in the recessed ring, and the bottom of the tank body is arranged in the central area. In this way, the liquid tank can be well fixed on the receiving plate.

[0025] Furthermore, in order to prevent the receiving plate from shaking during use, a circular hole with the same outer diameter as the blocking ring is provided, and the receiving plate is provided inside the circular hole, and the side wall of the circular hole limits the shaking of the receiving plate; through this arrangement, the stability of the entire liquid tank during use is further maintained by maintaining the stability of the receiving plate.

[0026] Furthermore, a containing structure for accommodating the weighing structure is provided, a cavity is provided in the containing structure, the bearing structure is provided in the cavity, and a circular hole consistent with the outer diameter of the blocking ring is provided on the containing structure. Through this arrangement, the weighing structure can be uniformly fixed or movably provided to the bottom of the incubator.

[0027] Furthermore, in order to ensure the operation of the entire liquid tank when cleaning the tank body and the tank cover, the liquid tank is fixed to the edge of the bottom of the incubator, and the liquid tank is set as a movable tank body that can be moved out of the incubator. The containment structure is set as a structure that can be translated; a track groove equal to the width of the containment structure is set at the bottom of the incubator, the track groove is the groove body in the middle of the track structure, and track 1 is set on the side walls of the track structure. Correspondingly, track 2 adapted to track 1 is set on the containment structure. The containment structure is moved out of the incubator with the liquid tank by relative movement of track 1 relative to track 2.

[0028] Technical Effects

[0029] By setting up a structure for weighing the liquid tank in real time and controlling the liquid inlet and liquid outlet processes through a control structure, this setting can simplify the monitoring process of the liquid in the liquid tank, so that the liquid inlet and liquid outlet do not need to be carried out simultaneously, reducing the difficulty of monitoring; in addition, when culturing different groups of hollow fiber bioreactors, only the theoretical weight value and the fluctuation value need to be adjusted to meet the monitoring needs of different groups of hollow fiber bioreactors, which can be very conveniently adjusted and monitored according to needs.

[0030] The setting of the double liquid passages can well achieve the purpose of injecting fresh nutrient liquid to realize the renewal of the liquid in the liquid tank, and can well meet the cultivation needs of the hollow fiber bioreactor.

[0031] The support frame and inclined support feet of the liquid tank can increase the bottom area to improve the stability of the liquid tank when it is weighed and placed. The support frame and the liquid tank are combined into a closed and stable whole. The monitoring is completed by monitoring the weight of the support frame, the liquid tank and the liquid inside. This makes the monitoring easier and increases the stability of the liquid tank.

[0032] By arranging a recessed ring and a blocking ring for accommodating the inclined support feet on the receiving plate of the weighing structure, the stability of the liquid tank after placement can be further ensured, and the validity of the monitored weight of the liquid tank can be ensured.

[0033] By arranging the containing structure for containing the weighing structure as a translatable structure, it can be ensured that the liquid tank is moved out of the incubator along with the containing structure, making the initial or post-cleaning process of the liquid tank simpler. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a structural schematic diagram of a state in which a liquid tank is set to a weighing structure and the liquid tank is not removed according to the present invention;

[0035] Figure 2 It is a structural schematic diagram of a liquid tank being set to a weighing structure and the liquid tank being removed according to the present invention;

[0036] Figure 3 This is a schematic diagram of the structure of a liquid tank provided with a support frame according to the present invention;

[0037] Figure 4 It is a schematic diagram of the weighing structure with a track structure of the present invention;

[0038] Figure 5 It is a structural schematic diagram of a weighing structure with a track structure of the present invention and a weighing structure in a removed state;

[0039] Figure 6 It is a longitudinal cross-sectional schematic diagram of a weighing structure with a track structure of the present invention;

[0040] Figure 7 It is a schematic diagram of the longitudinal cross-sectional structure of the weighing structure of the present invention;

[0041] Figure 8 It is a schematic diagram of the flow structure of the two liquid passages of the present invention;

[0042] Fig. 9 It is a structural schematic diagram of the liquid monitoring, liquid input and output process in the liquid tank of the present invention;

[0043] Fig.10 It is a schematic diagram of the structure of a culture box with a liquid tank and a hollow fiber bioreactor of the present invention;

[0044] Fig.11 It is a schematic diagram of the structure of a culture box without a liquid tank and a hollow fiber bioreactor of the present invention;

[0045] Fig.12 It is a schematic diagram of the structure of the combination of the incubator and the incubator rack of the present invention;

[0046] Fig.13 It is a schematic diagram of the partial structure of the culture plate of the present invention;

[0047] Fig.14 This is a schematic diagram of the support frame structure of the present invention;

[0048] Fig.15 This is a schematic diagram of the first supporting structure of the present invention;

[0049] Description of main reference numerals

[0050] 1. Liquid tank; 11. Tank body; 12. Tank cover; 121. Nutrient solution inlet connector; 122. Nutrient solution outlet connector; 123. Reactor inlet connector; 124. Reactor outlet connector; 131. Support rod; 132. Inclined support foot; 133. Fixing ring; 134. Fixing hole; 135. Locking screw; 136. Nut; 137. Connecting ring; 138. Abutting screw; 2. Weighing structure; 2. Weighing structure; 21. Weighing module; 22. Receiving plate; 221. Center area; 222. Depression Ring; 223, blocking ring; 224, containing structure; 2241, circular hole; 2251, track groove; 2252, track one; 2253, track two; 3, culture rack; 311, first plate; 312, second plate; 313, rotating column; 314, extension plate; 315, locking hole one; 321, first supporting structure; 322, second supporting structure; 323, lateral supporting structure; 324, rotating groove; 325, locking hole two; 331, locking rod; 4, incubator; 5, hollow fiber bioreactor. DETAILED DESCRIPTION

[0051] Example 1

[0052] A liquid tank system 1 capable of effectively monitoring the working fluid condition, comprising:

[0053] The liquid tank 1 comprises a tank body 11 and a tank cover 12. Various openings are arranged on the tank cover 12 for arranging various connectors. The volume of the tank body 11 is sufficient to meet the volume of the nutrient solution required for culturing at least one group of hollow fiber bioreactors 5.

[0054] The weighing structure is used to weigh the weight of the liquid in the liquid tank 1 and its tank body 11 in real time to obtain the actual weight value;

[0055] The control structure receives the actual weight value of the weighing structure in real time and compares it with the theoretical weight value. The theoretical weight value is the sum of the weight of the tank body 11 and the weight of the liquid required for culturing the required number of hollow fiber bioreactors 5, and there is a fluctuation value of the theoretical weight. When the actual weight value is the difference between the theoretical weight value and the fluctuation value, the control structure controls the input of nutrient solution into the tank body 11. When the actual weight value is the sum of the theoretical weight value and the fluctuation value, the control structure controls the liquid in the tank body 11 to flow out, and the process repeats continuously.

[0056] The above-mentioned setting can well maintain the liquid in the tank body 11 within the threshold range of the theoretical weight value and maintain a relative equilibrium state, but the situation of simultaneous liquid mixing will not occur, providing an effective buffer stage for liquid mixing and avoiding liquid waste caused by improper liquid mixing. The most important advantage is that if the volume of the liquid tank 1 meets the culture requirements of 3 or 4 groups of hollow fiber bioreactors 5, the working liquid volume can be monitored directly through the theoretical weight value, and the conversion is simple and convenient. A larger liquid tank 1 can meet the monitoring needs of any number of groups of culture. It should be noted that the number of bioreactors in one group of hollow fiber bioreactors 5 is the number required to meet the needs of an in vitro liver support system, which is generally set to 4, so one group is 4 hollow fiber bioreactors 5, which can be adjusted according to the needs of the operation.

[0057] In a more preferred embodiment, the fluctuation value ranges from 50 to 100 g, which can meet the needs of culture medium fluctuations without causing any impact.

[0058] In a more preferred embodiment, the time required to complete a round of liquid replacement is adjusted by adjusting the speed of inputting and outputting the nutrient solution into and out of the tank body 11 according to the conditions of the nutrient components in the tank body 11. In this way, the rate of completing a round of liquid replacement is adjusted.

[0059] In a more preferred embodiment, the tank cover 12 of the liquid tank 1 is provided with a nutrient solution inlet joint 121 for introducing the nutrient solution in the nutrient solution delivery structure into the tank body 11; a nutrient solution outlet joint 122 for leading the nutrient solution from the tank body 11 into the waste liquid collection structure; a reactor inlet joint 123 for delivering the working liquid in the tank body 11 to the hollow fiber bioreactor 5; a reactor outlet joint 124 for introducing the liquid in the hollow fiber bioreactor 5 into the tank body 11; wherein the reactor inlet flow rate is the same as the reactor outlet flow rate; two liquid passages are constructed by the arrangement of the above-mentioned joints, one is liquid passage 1 between the liquid tank 1 and the nutrient solution delivery structure and the waste liquid collection structure; the other is liquid passage 2 for the circulation of the liquid in the hollow fiber bioreactor 5 and the liquid tank 1; effective cultivation of the hollow fiber bioreactor 5 is achieved through these two liquid passages, wherein liquid passage 1 realizes the replacement of the liquid in the tank body 11, and liquid passage 2 directly provides nutrients to the hollow fiber bioreactor 5. Liquid passage 1 and liquid passage 2 are both provided with power pumps, wherein the pump of liquid passage 2 is a gear pump, which replaces the original magnetic pump. This arrangement can save the liquid filling process during the calibration process.

[0060] In a more preferred embodiment, the nutrient solution inlet joint 121 and the reactor inlet joint 123 extend into the tank body 11 and are close to each other in the tank body 11, and the nutrient solution outlet joint 122 and the reactor outlet joint 124 extend into the tank body 11 and are close to each other in the tank body 11. This arrangement ensures that the newly entered fresh nutrient solution enters the hollow fiber bioreactor 5. In a more preferred embodiment, the nutrient solution inlet joint 121 and the reactor inlet joint 123 extend to the bottom of the tank body 11, and the nutrient solution outlet joint 122 and the reactor outlet joint 124 are also arranged at the bottom of the tank body 11. This arrangement can ensure that the flow of the incoming liquid drives the flow of the liquid in the entire tank body 11, and ensures that the liquid in the tank body 11 is affected, and the reason for arranging the nutrient solution outlet joint 122 at the bottom of the tank body 11 is that the liquid can be completely led out to the waste liquid collection structure.

[0061] In a more preferred embodiment, the other joints on the tank cover 12 extend to the position where the liquid surface can be touched in the middle of the tank body 11. This arrangement can ensure that various indicators of the liquid in the tank body 11 are monitored, ensure the safety of the nutrient solution, and make timely adjustments.

[0062] A more preferred implementation manner is to carry out real-time weighing throughout the entire process, and in order to ensure the stability of the overall structure during weighing, a support frame is provided outside the liquid tank 1, and inclined support feet 132 are provided at the bottom of the support frame. By providing the inclined support feet 132, the stability of the liquid tank 1 when placed can be increased by increasing the supporting area, and the weight of the support frame is also within the weighing range during weighing, as part of the initial weight of the liquid tank 1.

[0063] In a more preferred embodiment, the support frame includes a fixing ring 133 arranged on the top, the inner diameter of the fixing ring 133 is smaller than the outer diameter of the edge of the tank body 11, and the diameter of the fixing ring 133 is equal to the outer diameter of the tank cover 12, and a sealing ring is also arranged on the tank body 11; the fixing ring 133 and the tank cover 12 are fixed together, and the sealing ring realizes the sealing of the tank cover 12 and the tank body 11; in this way, the support frame and the tank body 11 are stably combined together to form a whole that can be weighed together. In this way, the stability of the liquid tank 1 when placed, as well as the effectiveness and operability of the weighing can be maximized.

[0064] In a more preferred embodiment, corresponding fixing holes 134 are set on the fixing ring 133 and the tank cover 12, and locking screws 135 and nut 136 are set. The locking screw 135 is passed through the fixing hole 134, and the position of the fixing ring 133 and the tank cover 12 is locked by the screw and nut 136.

[0065] In a more preferred embodiment, the support frame includes multiple support rods 131, an inclined rod-shaped support foot is arranged under each support rod 131, a fixing ring 133 is arranged above the support rod 131, and a connecting ring 137 fixed to the inner side of the multiple support rods 131 is arranged under the support rod 131. The upper fixing ring 133 and the lower connecting ring 137 are combined with the support rod 131 to construct a stable support frame.

[0066] In a more preferred embodiment, abutment screws 138 are further provided at the bottom of each support rod 131 for abutting the tank body 11 at the bottom, so that the support frame and the tank body 11 form a more stable structure.

[0067] In a more preferred embodiment, the weighing structure 22 includes a weighing module 21, on which a receiving plate 22 is arranged for receiving the liquid tank 1. The receiving plate 22 is arranged as a circular plate, and a central area 221 and a recessed ring 222 which is lower than the central area 221 and is arranged on the circular plate for receiving the supporting feet of the supporting frame. A blocking ring 223 which is arranged perpendicular to the recessed ring 222 is also arranged on the outer side of the recessed ring 222; the supporting feet are arranged in the recessed ring 222, and the bottom of the tank body 11 is arranged in the central area 221. In this way, the liquid tank 1 can be well fixed on the receiving plate 22.

[0068] In a more preferred embodiment, in order to avoid the shaking of the receiving plate 22 during use, a circular hole 2241 is provided with the same outer diameter as the blocking ring 223, and the receiving plate 22 is provided inside the circular hole 2241, and the side wall of the circular hole 2241 limits the shaking of the receiving plate 22; through this arrangement, the stability of the receiving plate 22 is further maintained to achieve the purpose of maintaining the stability of the entire liquid tank 1 during use.

[0069] In a more preferred embodiment, a containing structure 224 is provided to contain the weighing structure 22, a cavity is provided in the containing structure 224, a bearing structure is provided in the cavity, and a circular hole 2241 which is consistent with the outer diameter of the blocking ring 223 is provided on the containing structure 224. Through this arrangement, the weighing structure 22 can be uniformly fixed or movably set to the bottom of the incubator 4.

[0070] In a more preferred embodiment, in order to ensure the operation of the entire liquid tank 1 when cleaning the tank body 11 and the tank cover 12, the liquid tank 1 is fixed to the edge position of the bottom of the incubator 4, and the liquid tank 1 is configured as a movable tank body 11 that can be moved out of the incubator 4.

[0071] In a more preferred embodiment, the containing structure 224 is configured as a structure capable of translation; a track groove 2251 equal to the width of the containing structure 224 is provided at the bottom of the incubator 4, the track groove 2251 is the groove body in the middle of the track structure, and a track 1 2252 is provided on the side walls of the track structure, and a track 2 2253 adapted to the track 1 2252 is correspondingly provided on the containing structure 224, and the purpose of moving the containing structure 224 with the liquid tank 1 out of the incubator 4 is achieved by the relative movement of the track 1 2252 with respect to the track 2 2253.

[0072] In a more preferred embodiment, magnetic structures are correspondingly arranged at the bottom of the containing structure 224 and on the track structure, so as to lock the position of the containing structure 224 relative to the track structure after the containing structure 224 enters the incubator 4, thereby preventing the containing structure 224 from moving relative to the track structure in the absence of external force.

[0073] Example 2

[0074] A stably placed liquid tank 1 comprises the liquid tank 1 and a support frame arranged outside the liquid tank 1, wherein inclined support legs 132 are arranged at the bottom of the support frame. The inclined support legs 132 can increase the support area to increase the stability of the liquid tank 1 when it is placed, and the weight of the support frame is also within the weighing range during weighing as part of the initial weight of the liquid tank 1.

[0075] In a more preferred embodiment, the support frame includes a fixing ring 133 arranged on the top, the inner diameter of the fixing ring 133 is smaller than the outer diameter of the edge of the tank body 11, and the diameter of the fixing ring 133 is equal to the outer diameter of the tank cover 12, and a sealing ring is also arranged on the tank body 11; the fixing ring 133 is fixed to the tank cover 12, and the sealing ring realizes the sealing of the tank cover 12 and the tank body 11; in this way, the support frame and the tank body 11 are stably combined together to form a whole that can be weighed together, and in this way, the stability of the liquid tank 1 when placed can be achieved to the greatest extent.

[0076] In a more preferred embodiment, corresponding fixing holes 134 are set on the fixing ring 133 and the tank cover 12, and locking screws 135 and nut 136 are set. The locking screw 135 is passed through the fixing hole 134, and the position of the fixing ring 133 and the tank cover 12 is locked by the screw and nut 136.

[0077] In a more preferred embodiment, the support frame includes multiple support rods 131, an inclined rod-shaped support foot is arranged under each support rod 131, a fixing ring 133 is arranged above the support rod 131, and a connecting ring 137 fixed to the inner side of the multiple support rods 131 is arranged under the support rod 131. The upper fixing ring 133 and the lower connecting ring 137 are combined with the support rod 131 to construct a stable support frame.

[0078] In a more preferred embodiment, abutment screws 138 are further provided at the bottom of each support rod 131 for abutting the tank body 11 at the bottom, so that the support frame and the tank body 11 form a more stable structure.

[0079] Example 3

[0080] A weighing structure 22 for weighing a liquid tank 1 includes a weighing module 21 and a receiving plate 22. The receiving plate 22 is arranged on the weighing module 21. The receiving plate 22 is arranged as a circular plate. A central area 221 and a recessed ring 222 which is lower than the central area 221 and is arranged on the circular plate, and is used to receive a support foot of a support frame. A blocking ring 223 which is arranged perpendicular to the recessed ring 222 is also arranged on the outer side of the recessed ring 222. The support foot is arranged in the recessed ring 222, and the bottom of the tank body 11 is arranged in the central area 221. In this way, the liquid tank 1 can be well fixed on the receiving plate 22.

[0081] In a more preferred embodiment, in order to avoid the shaking of the receiving plate 22 during use, a circular hole 2241 is provided with the same outer diameter as the blocking ring 223, and the receiving plate 22 is provided inside the circular hole 2241, and the side wall of the circular hole 2241 limits the shaking of the receiving plate 22; through this arrangement, the stability of the receiving plate 22 is further maintained to achieve the purpose of maintaining the stability of the entire liquid tank 1 during use.

[0082] In a more preferred embodiment, a containing structure 224 is provided to contain the weighing structure 22, a cavity is provided in the containing structure 224, a bearing structure is provided in the cavity, and a circular hole 2241 which is consistent with the outer diameter of the blocking ring 223 is provided on the containing structure 224. Through this arrangement, the weighing structure 22 can be uniformly fixed or movably set to the bottom of the incubator 4.

[0083] In a more preferred embodiment, in order to ensure the operation of the entire liquid tank 1 when cleaning the tank body 11 and the tank cover 12, the liquid tank 1 is fixed to the edge position of the bottom of the incubator 4, and the liquid tank 1 is configured as a movable tank body 11 that can be moved out of the incubator 4.

[0084] In a more preferred embodiment, the containing structure 224 is configured as a structure capable of translation; a track groove 2251 equal to the width of the containing structure 224 is provided at the bottom of the incubator 4, the track groove 2251 is the groove body in the middle of the track structure, and a track 1 2252 is provided on the side walls of the track structure, and a track 2 2253 adapted to the track 1 2252 is correspondingly provided on the containing structure 224, and the purpose of moving the containing structure 224 with the liquid tank 1 out of the incubator 4 is achieved by the relative movement of the track 1 2252 with respect to the track 2 2253.

[0085] In a more preferred embodiment, magnetic structures are correspondingly arranged at the bottom of the containing structure 224 and on the track structure, so as to lock the position of the containing structure 224 relative to the track structure after the containing structure 224 enters the incubator 4, thereby preventing the containing structure 224 from moving relative to the track structure in the absence of external force.

[0086] Example 4

[0087] A hollow fiber bioreactor 5 culture system comprises a sealed box, a hollow fiber bioreactor 5 culture rack 3 with an adjustable angle arranged in the box, and the liquid tank 1 system described in Example 1.

[0088] The culture rack 3 includes:

[0089] The culture plate is provided with a rotating column 313; it is used to set up multiple groups of hollow fiber bioreactors 5;

[0090] The receiving frame is provided with a rotation groove 324; the rotation column 313 of the culture plate is arranged in the rotation groove 324 of the supporting structure to realize the combination of the culture plate and the supporting structure and the adjustment of the angle of the culture plate, so that the culture plate can reach a horizontal state and an inclined state;

[0091] The angle adjustment and locking structure is used to lock the positional relationship between the culture plate and the supporting structure when the culture plate reaches a horizontal state or an inclined state, and to release it when adjustment is needed to adjust the relative positional relationship between the culture plate and the supporting structure so that the culture plate can switch between a horizontal state and an inclined state.

[0092] In a more preferred embodiment, the culture plate includes a top plate and surrounding plates, which are arranged around the top plate, and the surrounding plates include a first plate 311 and a second plate 312. A rotating column 313 extends from each of the first plate 311 and the second plate 312, and a rotating groove 324 for accommodating the rotating column 313 is correspondingly provided on the receiving frame; in a more preferred embodiment, the rotating groove 324 includes a guide groove with a larger opening at the top and an adapting groove at the bottom that is adapted to the rotating column 313. In this way, the culture plate can be very conveniently placed on the receiving frame.

[0093] In a more preferred embodiment, the receiving frame includes a first supporting structure 321, a second supporting structure 322, and also includes a transverse supporting structure 323 connecting the first supporting structure 321 and the second supporting structure 322. The receiving frame is fixed to the incubator 4 through the transverse supporting structure 323, and a rotating groove 324 is respectively provided on the first supporting structure 321 and the second supporting structure 322.

[0094] The angle adjustment and locking structure includes an extension plate 314 arranged on the first plate 311 and / or the second plate 312, two locking holes 315 in a horizontal state and an inclined state are arranged on the extension plate 314, a locking hole 325 cooperating with the locking hole 315 is arranged on one of the first supporting structure 321 and the second supporting structure 322, and a locking rod 331 is also arranged. When one locking hole 315 is aligned with the second locking hole 325, the locking rod 331 is inserted into the hole to lock the angle of the culture plate, and when the locking rod 331 leaves the locking hole 315, adjustment is performed.

[0095] In a more preferred embodiment, the distances between the two locking holes 1 315 and the axis of the rotating column 313 are equal, and the locking hole 2 325 is arranged on the locking plate outside the rotating groove 324 .

[0096] In a more preferred embodiment, the rotating column 313 is arranged in the middle position of the culture plate, the culture plate is a plane-symmetrical figure, and the central axis of the rotating column 313 is on the longitudinal symmetry plane; this arrangement can ensure the arbitrariness of the culture plate arrangement without specially distinguishing the up and down directions.

[0097] In a more preferred embodiment, two sets of locking holes 315 are provided on the first plate 311 and the second plate 312 with the longitudinal symmetry plane as a mirror plane. This arrangement ensures that an effective horizontal state and an inclined state can be found in any placement method.

[0098] The technical solutions in the embodiments of the present invention are clearly and completely described above through specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation methods. In the absence of conflict, the above embodiments and features in the embodiments can be combined with each other. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

Claims

1. A liquid tank system capable of effectively monitoring the working fluid condition, comprising: The liquid tank comprises a tank body and a tank cover. Various openings are arranged on the tank cover for arranging various connectors. The volume of the tank body is sufficient to meet the volume of the nutrient solution required for culturing at least one group of hollow fiber bioreactors. A weighing structure is used to weigh the liquid tank and the liquid in the tank in real time to obtain the actual weight value; The control structure receives the actual weight value of the weighing structure in real time and compares it with the theoretical weight value. The theoretical weight value is the sum of the tank weight and the liquid weight required for culturing the required number of hollow fiber bioreactors, and there is a fluctuation value of the theoretical weight. When the actual weight value is the difference between the theoretical weight value and the fluctuation value, the control structure controls the input of nutrient solution into the tank. When the actual weight value is the sum of the theoretical weight value and the fluctuation value, the control structure controls the liquid out of the tank, and the process repeats itself.

2. The liquid tank system according to claim 1, characterized in that The tank cover of the liquid tank is provided with a nutrient solution inlet joint, which is used to introduce the nutrient solution in the nutrient solution delivery structure into the tank body, and a nutrient solution outlet joint, which is used to lead the nutrient solution from the tank body to the waste liquid collection structure; a reactor inlet joint, which is used to transport the working liquid in the tank body to the hollow fiber bioreactor; a reactor outlet joint, which is used to introduce the liquid in the hollow fiber bioreactor into the tank body; wherein the reactor inlet flow rate is the same as the reactor outlet flow rate.

3. The liquid tank system according to claim 1, characterized in that A support frame is arranged outside the liquid tank, and inclined support feet are arranged at the bottom of the support frame. When weighing, the weight of the support frame is also within the weighing range as part of the initial weight of the liquid tank; Preferably, the support frame includes a fixing ring arranged on the top, the inner diameter of the fixing ring is smaller than the outer diameter of the edge of the tank body, and the diameter of the fixing ring is equal to the outer diameter of the tank cover, and a sealing ring is also arranged on the tank body; the fixing ring and the tank cover are fixed together, and the sealing ring realizes the sealing between the tank cover and the tank body; Preferably, corresponding fixing holes are provided on the fixing ring and the tank cover, and locking screws and nuts are provided, the locking screws are passed through the fixing holes, and the positions of the fixing ring and the tank cover are locked by the screws and nuts; Preferably, the support frame comprises a plurality of support rods, an inclined rod-shaped support foot is arranged below each support rod, a fixing ring is arranged above the support rod, and a connecting ring fixed to the inner side of the plurality of support rods is arranged below the support rod; Preferably, an abutment screw is also provided at the bottom of each support rod for abutting the tank body at the bottom, so that the support frame and the tank body form a more stable structure.

4. The liquid tank system according to claim 3, characterized in that: The weighing structure includes a receiving plate for receiving the liquid tank. The receiving plate is set as a circular plate. A central area and a concave ring lower than the central area are set on the circular plate for receiving the support feet of the support frame. A blocking ring perpendicular to the concave ring is also set on the outer side of the concave ring. The support feet are set in the concave ring, and the bottom of the tank body is set in the central area. In this way, the liquid tank can be well fixed on the receiving plate. Preferably, a circular hole having the same outer diameter as the blocking ring is provided, a receiving plate is provided inside the circular hole, and the side wall of the circular hole restricts the receiving plate from shaking.

5. The liquid tank system according to claim 4, characterized in that: A containing structure for containing the weighing structure is provided, a cavity is provided in the containing structure, the bearing structure is provided in the cavity, and a circular hole having the same outer diameter as the blocking ring is provided on the containing structure.

6. The liquid tank system according to claim 5, characterized in that The liquid tank is fixed to the edge of the bottom of the incubator, and the liquid tank is configured as a movable tank body that can be moved out of the incubator; Preferably, the containing structure is configured as a structure capable of translation, and a track 1 is provided on both side walls of the track structure, and a track 2 adapted to the track 1 is correspondingly provided on the containing structure, and the purpose of moving the containing structure with the liquid tank out of the incubator is achieved by relative movement of the track 1 relative to the track 2; Preferably, magnetic structures are provided at the bottom of the containing structure and on the track structure correspondingly, so as to lock the position of the containing structure relative to the track structure after the containing structure enters the incubator.

7. A stably placed liquid tank for a liquid tank system according to any one of claims 1 to 6, characterized in that: It includes a liquid tank and a support frame arranged outside the liquid tank, and an inclined support foot is arranged at the bottom of the support frame; Preferably, the support frame includes a fixing ring arranged on the top, the inner diameter of the fixing ring is smaller than the outer diameter of the edge of the tank body, and the diameter of the fixing ring is equal to the outer diameter of the tank cover, and a sealing ring is also arranged on the tank body; the fixing ring and the tank cover are fixed together, and the sealing ring realizes the sealing between the tank cover and the tank body; Preferably, the support frame includes multiple support rods, an inclined rod-shaped support foot is arranged under each support rod, a fixing ring is arranged above the support rod, and a connecting ring fixed to the inner side of the multiple support rods is arranged under the support rod. The upper fixing ring and the lower connecting ring are combined with the support rod to construct a stable support frame.

8. A weighing structure for a liquid tank system according to any one of claims 1 to 6, comprising a weighing module and a receiving plate, the receiving plate being arranged on the weighing module, the receiving plate being arranged as a circular plate, a central area and a concave ring at the periphery of the central area being arranged on the circular plate, which is lower than the central area and is used to receive the support legs of the support frame, and a blocking ring arranged perpendicular to the concave ring is also arranged on the outer side of the concave ring; the support legs are arranged in the concave ring, and the bottom of the tank body is arranged in the central area, so that the liquid tank can be well fixed on the receiving plate in this way; Preferably, a circular hole having the same outer diameter as the blocking ring is provided, a receiving plate is provided inside the circular hole, and the side wall of the circular hole restricts the receiving plate from shaking; A containing structure for containing the weighing structure is provided, a cavity is provided in the containing structure, the bearing structure is provided in the cavity, and a circular hole having the same outer diameter as the blocking ring is provided on the containing structure; Preferably, the containing structure is configured as a structure that can move horizontally; a track groove equal to the width of the containing structure is provided at the bottom of the incubator, the track groove is the groove body in the middle of the track structure, and track one is provided on the side walls of the track structure, and track two adapted to track one is correspondingly provided on the containing structure, and the purpose of moving the containing structure with the liquid tank out of the incubator is achieved by relative movement of track one with respect to track two.

9. A hollow fiber bioreactor culture system, comprising a sealed box, characterized in that: A hollow fiber bioreactor culture rack with adjustable angle arranged in the box, and a liquid tank system according to any one of claims 1-6.

10. The culture system according to claim 9, characterized in that: The culture rack includes: A culture plate is provided with a rotating column; it is used to set up multiple groups of hollow fiber bioreactors; The receiving frame is provided with a rotating groove; the rotating column of the culture plate is arranged in the rotating groove of the supporting structure to realize the combination of the culture plate and the supporting structure and the adjustment of the angle of the culture plate, so that the culture plate can reach a horizontal state and an inclined state; The angle adjustment and locking structure is used to lock the positional relationship between the culture plate and the supporting structure when the culture plate reaches a horizontal state or an inclined state, and to release it when adjustment is needed to adjust the relative positional relationship between the culture plate and the supporting structure so that the culture plate can switch between a horizontal state and an inclined state.

Images