Method and device for purifying glucose oxidase

Abstract

The invention relates to the technical field of glucose oxidase production equipment, and discloses a method and device for purifying glucose oxidase, the device comprises a mounting plate and a vibration assembly arranged above the mounting plate, the vibration assembly comprises a fixing frame fixedly connected to the bottom of the mounting plate, and the fixing frame is fixedly connected to the bottom of the mounting plate; the inner wall of the other end of the fixing frame is fixedly connected with a first motor, the output end of the first motor is fixedly connected with a first output shaft, the outer wall of the top of the first output shaft is fixedly sleeved with a driving gear, the side wall of the driving gear is meshed with a driven gear, and the inner wall of the driven gear is fixedly connected with a transmission shaft; the outer wall of the middle part of the transmission shaft is rotatably connected with an L-shaped connecting plate through a bearing, and by arranging a vibration assembly, a second convex block can be extruded through a first convex block, so that the mixing barrel is vibrated, and glucose oxidase and a diluent which are attached to the inner wall of the mixing barrel in the mixing process are vibrated off.

Description

Technical Field

[0001] The invention relates to the technical field of glucose oxidase production equipment, in particular to a method and a device for purifying glucose oxidase. Background Art

[0002] Glucose oxidase is an enzyme found in molds such as Penicillium insolens and honey. It can catalyze the reaction of D-glucose + O2D-gluconic acid (δ-lactone) + H2O2, EC1.1.3.4. The enzyme of Penicillium insolens has attracted attention for its obvious antibacterial property, so it is also called glucose oxidase. It is now clear that the antibacterial property is due to the bactericidal effect of H2O2 generated by the reaction.

[0003] According to a glucose oxidase anti-clogging purification device proposed in Chinese patent CN218306500U, in the device, the glucose oxidase and the diluent in the fermentation tank are fully mixed by the rotation of the stirring shaft, and filtered through the filter cartridge. The glucose oxidase dissolved in the diluent is discharged through the filter cartridge for purification, while impurities are retained in the filter cartridge. The impurities on the inner surface of the filter cartridge are cleaned by a scraper as the stirring shaft rotates, thereby avoiding the problem of filter cartridge clogging.

[0004] However, there are still some shortcomings in this patent. The stirring shaft and stirring blades in this device are all located at the lower part of the inside of the fermentation tank. When the stirring shaft and stirring blades rotate to mix the glucose oxidase and the diluent, it is easy to cause the glucose oxidase and the diluent to adhere to the inner wall of the fermentation tank. The glucose oxidase and the diluent adhered to the inner wall of the fermentation tank will slowly fall along the inner wall of the fermentation tank, thereby affecting the mixing efficiency of the glucose oxidase and the diluent, and reducing the purification efficiency of the glucose oxidase of the filter cartridge below. For this reason, we propose a method and device for purifying glucose oxidase. Summary of the invention

[0005] The purpose of the present invention is to provide a method and device for purifying glucose oxidase, which solves the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a device for purifying glucose oxidase, comprising a mounting plate, and a vibration assembly arranged above the mounting plate, wherein the vibration assembly comprises a fixing frame fixedly connected to the bottom of the mounting plate, the inner wall of the other end of the fixing frame is fixedly connected to a first motor, the output end of the first motor is fixedly connected to a first output shaft, a driving gear is fixedly sleeved on the top outer wall of the first output shaft, a driven gear is meshed with a side wall of the driving gear, a transmission shaft is fixedly connected to the inner wall of the driven gear, and the middle outer wall of the transmission shaft is connected to the transmission shaft through the shaft The bearing is rotatably connected with an L-shaped connecting plate, the other end of the L-shaped connecting plate is fixedly connected to the bottom of the mounting plate, the top of the transmission shaft is fixedly connected with a circular plate, the top of the circular plate is fixedly connected with a first protrusion, the top of the first protrusion is slidably connected with a second protrusion, the top of the second protrusion is fixedly connected with a mixing barrel, the outer wall of the mixing barrel is fixedly connected with a connecting block, the inner wall of the connecting block is slidably connected with a limiting column, the bottom of the limiting column is fixedly connected with the top of the mounting plate, the middle outer wall of the limiting column is fixedly connected with a rectangular block, and the top of the rectangular block is fixedly connected with a first spring , the other end of the first spring is fixedly connected to the bottom of the limiting column. By setting a vibration component, in the process of mixing glucose oxidase and diluent, in order to prevent glucose oxidase and diluent from adhering to the inner wall of the mixing barrel, the mixing barrel needs to be vibrated, so as to achieve uniform mixing of glucose oxidase and diluent. At this time, the operator turns on the first motor to cause the first output shaft to rotate. During the rotation of the first output shaft, the driving gear will rotate, and the driving gear will mesh with the driven gear, thereby driving the transmission shaft to rotate. During the rotation of the transmission shaft, the circular plate will rotate, causing the first protrusion on the top of the circular plate to rotate. During the rotation of the first protrusion, the second protrusion will be squeezed to move the mixing barrel upward. When the first protrusion does not squeeze the second protrusion, the connecting block on the outer wall of the mixing barrel will move downward along the outer wall of the limiting column and compress the first spring. By repeating this process, the mixing barrel can be vibrated up and down, which is convenient for shaking off the glucose oxidase and diluent attached to the inner wall of the mixing barrel during the mixing process, thereby improving the mixing effect of glucose oxidase and diluent inside the mixing barrel.

[0007] Preferably, a mixing assembly is provided inside the mixing barrel, and the mixing assembly includes a second motor fixedly connected to the top of the mixing barrel, an output end of the second motor is fixedly connected to a second output shaft, the second output shaft movably passes through the top of the mixing barrel and extends to the inside of the mixing barrel, an outer wall of the second output shaft is fixedly connected to a stirring blade, an inner wall of the stirring blade is rotatably connected to a rotating rod through a bearing, and an outer wall of the rotating rod is fixedly connected to a rotating plate. By setting up the mixing assembly, when it is necessary to mix glucose oxidase and the diluent, an operator can add glucose oxidase and the diluent into the mixing barrel through the feed port, and then turn on the second motor to cause the second output shaft to rotate. During the rotation of the second output shaft, the stirring blade will be rotated with it, so that the glucose oxidase and the diluent can be evenly stirred and mixed by the stirring blade. During the rotation of the stirring blade, the rotating rod will rotate, so that the rotating plate can rotate, so that the glucose oxidase and the diluent can be moved, so as to facilitate the even stirring of the glucose oxidase and the diluent.

[0008] Preferably, the mixing assembly also includes a fixed frame fixedly connected to the outer wall of the stirring blade, the side inner wall of the fixed frame is slidably connected with a displacement rod, one end of the displacement rod is fixedly connected to a limiting block, the side wall of the limiting block is fixedly connected with a second spring, the other end of the second spring is fixedly connected to the inner wall of the fixed frame, and the other end of the displacement rod is fixedly connected to the side wall of the scraper. During the rotation of the stirring blade, the second spring and the displacement rod will be thrown out under the action of centrifugal force, thereby moving with the scraper. During the rotation of the stirring blade, the scraper will be rotated with it, so that the glucose oxidase and diluent attached to the inner wall of the mixing barrel can be scraped off by the scraper.

[0009] Preferably, the mixing assembly also includes a feed port connected to the top of the mixing barrel. This arrangement allows glucose oxidase and the diluent to be easily fed into the mixing barrel through the feed port, thereby achieving stirring and mixing of the glucose oxidase and the diluent inside the mixing barrel.

[0010] Preferably, the outer wall of the mixing barrel is provided with a filter assembly, and the filter assembly includes a discharge hopper connected to the outer wall of the mixing barrel, a first valve on the middle outer wall of the discharge hopper, and the other end of the discharge hopper is connected to a filter box, the inner wall of the filter box is fixedly connected with a filter plate, the outer wall of the filter box is connected to a discharge pipe, and the outer wall of the discharge pipe is fixedly connected with a second valve. By setting the filter assembly, after the glucose oxidase and the diluent are stirred and mixed, the operator can open the first valve to allow the mixture of glucose oxidase and the diluent to enter the interior of the filter box through the discharge hopper, and the mixture can be filtered and purified through the filter plate. After the purification of the mixed solution is completed, the operator can open the second valve to discharge the purified mixed solution from the discharge pipe.

[0011] Preferably, the bottom of the mounting plate is fixedly connected with a support leg, and there are four support legs. The four support legs are equal in size and fixedly connected to the four corners of the bottom of the mounting plate at equal distances. By setting four support legs, the mounting plate can be supported by the four support legs.

[0012] Preferably, there are two connecting blocks, the two connecting blocks are equal in size, and the two connecting blocks are symmetrically distributed along the center plane of the mixing barrel.

[0013] A method for purifying glucose oxidase comprises the following steps:

[0014] S1. First, glucose oxidase and diluent are put into the mixing barrel through the feed port;

[0015] S2, secondly, stirring and mixing the glucose oxidase and the diluent by means of a stirring blade and a rotating plate;

[0016] S3, then the first convex block is used to squeeze the second convex block, so that the mixing barrel is vibrated, thereby improving the mixing effect of the glucose oxidase and the diluent;

[0017] S4. Finally, the mixed solution is filtered through a filter plate to purify the mixed solution of glucose oxidase and the diluent.

[0018] The present invention provides a method and device for purifying glucose oxidase. The method and device for purifying glucose oxidase have the following beneficial effects:

[0019] (1) The device for purifying glucose oxidase is provided with a vibration component. In the process of mixing glucose oxidase with a diluent, in order to prevent the glucose oxidase and the diluent from adhering to the inner wall of the mixing barrel, the mixing barrel needs to be vibrated, so as to achieve uniform mixing of the glucose oxidase and the diluent. At this time, the operator turns on the first motor to cause the first output shaft to rotate. During the rotation of the first output shaft, the driving gear will rotate, and the driving gear will mesh with the driven gear, thereby causing the transmission shaft to rotate. During the rotation of the transmission shaft, the circular plate will rotate, causing the first protrusion on the top of the circular plate to rotate. During the rotation of the first protrusion, the second protrusion will be squeezed to move the mixing barrel upward. When the first protrusion does not squeeze the second protrusion, the connecting block on the outer wall of the mixing barrel will move downward along the outer wall of the limiting column and compress the first spring. This process is repeated to achieve the mixing barrel to vibrate up and down, so as to facilitate the shaking off of the glucose oxidase and the diluent attached to the inner wall of the mixing barrel during the mixing process, thereby improving the mixing effect of the glucose oxidase and the diluent inside the mixing barrel;

[0020] (2) The glucose oxidase purification device is provided with a mixing component. When it is necessary to mix the glucose oxidase with the diluent, the operator can add the glucose oxidase and the diluent into the mixing barrel through the feed port, and then turn on the second motor to cause the second output shaft to rotate. During the rotation of the second output shaft, the stirring blade will rotate with it, so that the glucose oxidase and the diluent can be evenly stirred and mixed by the stirring blade. During the rotation of the stirring blade, the rotating rod will rotate, so that the rotating plate will rotate, so that the glucose oxidase and the diluent can be moved, so as to facilitate the uniform stirring of the glucose oxidase and the diluent. During the rotation of the stirring blade, the second spring and the displacement rod will be thrown out under the action of centrifugal force, so as to move with the scraper. During the rotation of the stirring blade, the scraper will rotate with it, so that the glucose oxidase and the diluent attached to the inner wall of the mixing barrel can be scraped off by the scraper;

[0021] (3) The device for purifying glucose oxidase is provided with a filtering component. After the glucose oxidase and the diluent are stirred and mixed, the operator can open the first valve to allow the mixture of glucose oxidase and the diluent to enter the interior of the filter box through the discharge hopper, and the mixture is filtered and purified through the filter plate. After the mixture is purified, the operator can open the second valve to discharge the purified mixture from the discharge pipe. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0023] Figure 2 is a cross-sectional view of the present invention;

[0024] Figure 3 It is a schematic diagram of the structure of the vibration component in the present invention;

[0025] Figure 4 It is a schematic diagram of the side structure of the vibration component in the present invention;

[0026] Figure 5 for Figure 4 A partial enlarged view of the middle A;

[0027] Figure 6 is a cross-sectional view of a mixing assembly in the present invention;

[0028] Figure 7 It is a schematic diagram of the local structure of the mixing component in the present invention;

[0029] Figure 8 It is a cross-sectional view of the filter assembly in the present invention.

[0030] In the figure: 1, mounting plate; 2, supporting leg; 31, vibration assembly; 311, fixing frame; 312, first motor; 313, first output shaft; 314, driving gear; 315, driven gear; 316, transmission shaft; 317, L-shaped connecting plate; 318, circular plate; 319, first protrusion; 3110, second protrusion; 3111, mixing barrel; 3112, connecting block; 3113, limiting column; 3114, rectangular block; 3115, first spring; 32. Mixing assembly; 321. Feed inlet; 322. Second motor; 323. Second output shaft; 324. Stirring blade; 325. Rotating rod; 326. Rotating plate; 327. Fixed frame; 328. Displacement rod; 329. Limiting block; 3210. Second spring; 3211. Scraper; 33. Filter assembly; 331. Discharge hopper; 332. First valve; 333. Filter box; 334. Filter plate; 335. Discharge pipe; 336. Second valve. DETAILED DESCRIPTION

[0031] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described with reference to the accompanying drawings.

[0032] A preferred embodiment of the device for purifying glucose oxidase provided by the present invention is as follows: Figures 1 to 8As shown: A device for purifying glucose oxidase, comprising a mounting plate 1, and a vibration assembly 31 arranged above the mounting plate 1, the vibration assembly 31 comprising a fixing frame 311 fixedly connected to the bottom of the mounting plate 1, the inner wall of the other end of the fixing frame 311 is fixedly connected to a first motor 312, the output end of the first motor 312 is fixedly connected to a first output shaft 313, the top outer wall of the first output shaft 313 is fixedly sleeved with a driving gear 314, the side wall of the driving gear 314 is meshed with a driven gear 315, the inner wall of the driven gear 315 is fixedly connected to a transmission shaft 316, the middle outer wall of the transmission shaft 316 is rotatably connected to an L-shaped connecting plate 317 through a bearing, the other end of the L-shaped connecting plate 317 is connected to the mounting plate 1 is fixedly connected to the bottom of the transmission shaft 316, the top of the transmission shaft 316 is fixedly connected to a circular plate 318, the top of the circular plate 318 is fixedly connected to a first protrusion 319, the top of the first protrusion 319 is slidably connected to a second protrusion 3110, the top of the second protrusion 3110 is fixedly connected to a mixing barrel 3111, the outer wall of the mixing barrel 3111 is fixedly connected to a connecting block 3112, the inner wall of the connecting block 3112 is slidably connected to a limiting column 3113, the bottom of the limiting column 3113 is fixedly connected to the top of the mounting plate 1, the middle outer wall of the limiting column 3113 is fixedly connected to a rectangular block 3114, the top of the rectangular block 3114 is fixedly connected to a first spring 3115, the other end of the first spring 3115 is fixedly connected to the limiting column The bottom of the mixing barrel 3113 is fixedly connected, and a vibration component 31 is provided. In the process of mixing the glucose oxidase and the diluent, in order to prevent the glucose oxidase and the diluent from adhering to the inner wall of the mixing barrel 3111, the mixing barrel 3111 needs to be vibrated, so as to achieve uniform mixing of the glucose oxidase and the diluent. At this time, the operator turns on the first motor 312 to cause the first output shaft 313 to rotate. During the rotation of the first output shaft 313, the driving gear 314 is rotated, and the driving gear 314 is meshed with the driven gear 315, thereby driving the transmission shaft 316 to rotate. During the rotation of the transmission shaft 316, the circular plate 318 is driven The first protrusion 319 on the top of the circular plate 318 rotates, and during the rotation of the first protrusion 319, the second protrusion 3110 is squeezed, causing the mixing barrel 3111 to move upward. When the first protrusion 319 stops squeezing the second protrusion 3110, the connecting block 3112 on the outer wall of the mixing barrel 3111 moves downward along the outer wall of the limiting column 3113 and compresses the first spring 3115. By repeating this process, the mixing barrel 3111 can be vibrated up and down, so that the glucose oxidase and the diluent attached to the inner wall of the mixing barrel 3111 during the mixing process can be shaken off, thereby improving the mixing effect of the glucose oxidase and the diluent inside the mixing barrel 3111.

[0033] A preferred embodiment of the device for purifying glucose oxidase provided by the present invention is as follows: Figures 1 to 8As shown: a mixing assembly 32 is arranged inside the mixing barrel 3111, and the mixing assembly 32 includes a second motor 322 fixedly connected to the top of the mixing barrel 3111, and the output end of the second motor 322 is fixedly connected to a second output shaft 323, and the second output shaft 323 movably passes through the top of the mixing barrel 3111 and extends to the inside of the mixing barrel 3111, and the outer wall of the second output shaft 323 is fixedly connected to a stirring blade 324, and the inner wall of the stirring blade 324 is rotatably connected to a rotating rod 325 through a bearing, and the outer wall of the rotating rod 325 is fixedly connected to a rotating plate 326. By setting the mixing assembly 32, when it is necessary to mix glucose oxidase with the diluent, The operator can add glucose oxidase and the diluent into the mixing barrel 3111 through the feed port 321, and then turn on the second motor 322 to cause the second output shaft 323 to rotate. During the rotation of the second output shaft 323, the stirring blade 324 will also rotate, so that the glucose oxidase and the diluent can be evenly stirred and mixed through the stirring blade 324. During the rotation of the stirring blade 324, the rotating rod 325 will rotate, so that the rotating plate 326 will rotate, so that the glucose oxidase and the diluent can be moved, so as to facilitate the uniform stirring of the glucose oxidase and the diluent.

[0034] The mixing assembly 32 also includes a fixed frame 327 fixedly connected to the outer wall of the stirring blade 324, and a displacement rod 328 is slidably connected to the inner wall of the side of the fixed frame 327, one end of the displacement rod 328 is fixedly connected to a limit block 329, and the side wall of the limit block 329 is fixedly connected to a second spring 3210, and the other end of the second spring 3210 is fixedly connected to the inner wall of the fixed frame 327, and the other end of the displacement rod 328 is fixedly connected to the side wall of the scraper 3211. During the rotation of the stirring blade 324, under the action of centrifugal force, the second spring 3210 and the displacement rod 328 will be thrown out, thereby moving with the scraper 3211. During the rotation of the stirring blade 324, the scraper 3211 will be rotated with it, so that the glucose oxidase and diluent attached to the inner wall of the mixing barrel 3111 can be scraped off by the scraper 3211.

[0035] The mixing assembly 32 also includes a feed port 321 connected to the top of the mixing barrel 3111. This arrangement allows glucose oxidase and the diluent to be easily fed into the mixing barrel 3111 through the feed port 321, thereby stirring and mixing the glucose oxidase and the diluent inside the mixing barrel 3111.

[0036] A preferred embodiment of the device for purifying glucose oxidase provided by the present invention is as follows: Figures 1 to 8As shown: the outer wall of the mixing barrel 3111 is provided with a filter assembly 33, the filter assembly 33 includes a discharge hopper 331 connected to the outer wall of the mixing barrel 3111, a first valve 332 on the middle outer wall of the discharge hopper 331, and the other end of the discharge hopper 331 is connected to a filter box 333, the inner wall of the filter box 333 is fixedly connected with a filter plate 334, the outer wall of the filter box 333 is connected to a discharge pipe 335, and the outer wall of the discharge pipe 335 is fixedly connected with a second valve 336. By setting the filter assembly 33, after the glucose oxidase and the diluent are stirred and mixed, the operator can open the first valve 332 to allow the mixed solution of the glucose oxidase and the diluent to enter the interior of the filter box 333 through the discharge hopper 331, and the mixed solution is filtered and purified through the filter plate 334. After the mixed solution is purified, the operator can open the second valve 336 to discharge the purified mixed solution from the discharge pipe 335.

[0037] Furthermore, a support leg 2 is fixedly connected to the bottom of the mounting plate 1. There are four support legs 2. The four support legs 2 are equal in size and are fixedly connected to the four corners of the bottom of the mounting plate 1 at equal distances. By setting four support legs 2, the mounting plate 1 can be supported by the four support legs 2.

[0038] In addition, there are two connecting blocks 3112 , the two connecting blocks 3112 are equal in size, and the two connecting blocks 3112 are symmetrically distributed along the center plane of the mixing barrel 3111 .

[0039] A method for purifying glucose oxidase comprises the following steps:

[0040] S1. First, glucose oxidase and diluent are put into the mixing barrel 3111 through the feed port 321;

[0041] S2, secondly, the glucose oxidase and the diluent are stirred and mixed by the stirring blade 324 and the rotating plate 326;

[0042] S3, then the second convex block 3110 is squeezed by the first convex block 319 to make the mixing barrel 3111 vibrate, thereby improving the mixing effect of the glucose oxidase and the diluent;

[0043] S4. Finally, the mixed solution is filtered through the filter plate 334 to purify the mixed solution of glucose oxidase and the diluent.

[0044] Working principle: In the process of mixing glucose oxidase and diluent, in order to prevent glucose oxidase and diluent from adhering to the inner wall of mixing barrel 3111, mixing barrel 3111 needs to be vibrated, so as to achieve uniform mixing of glucose oxidase and diluent. At this time, the operator turns on the first motor 312 to cause the first output shaft 313 to rotate. During the rotation of the first output shaft 313, the driving gear 314 is rotated, and the driving gear 314 is meshed with the driven gear 315, thereby causing the transmission shaft 316 to rotate. During the rotation of the transmission shaft 316, the circular plate 318 is rotated, causing the circular plate 31 The first protrusion 319 at the top rotates, and during the rotation of the first protrusion 319, the second protrusion 3110 is squeezed to make the mixing barrel 3111 move upward. When the first protrusion 319 stops squeezing the second protrusion 3110, the connecting block 3112 on the outer wall of the mixing barrel 3111 moves downward along the outer wall of the limiting column 3113 and compresses the first spring 3115. This process is repeated to achieve the up and down vibration of the mixing barrel 3111, so that the glucose oxidase and the diluent attached to the inner wall of the mixing barrel 3111 during the mixing process can be shaken off, thereby improving the mixing effect of the glucose oxidase and the diluent inside the mixing barrel 3111.

[0045] When it is necessary to mix the glucose oxidase and the diluent, the operator can add the glucose oxidase and the diluent into the mixing barrel 3111 through the feed port 321, and then turn on the second motor 322 to rotate the second output shaft 323. During the rotation of the second output shaft 323, the stirring blade 324 will be rotated, so that the glucose oxidase and the diluent can be evenly stirred and mixed by the stirring blade 324. During the rotation of the stirring blade 324, the rotating rod 325 will rotate, thereby realizing the rotation. The plate 326 rotates by itself, so that the glucose oxidase and the diluent can be moved, so that the glucose oxidase and the diluent can be evenly stirred. During the rotation of the stirring blade 324, the second spring 3210 and the displacement rod 328 are thrown out by the centrifugal force, so that the scraper 3211 is moved. During the rotation of the stirring blade 324, the scraper 3211 is rotated with it, so that the glucose oxidase and the diluent attached to the inner wall of the mixing barrel 3111 can be scraped off by the scraper 3211.

[0046] After the glucose oxidase and the diluent are stirred and mixed, the operator can open the first valve 332 to allow the mixture of glucose oxidase and the diluent to enter the interior of the filter box 333 through the discharge hopper 331, and the mixture can be filtered and purified through the filter plate 334. After the mixture is purified, the operator can open the second valve 336 to discharge the purified mixture from the discharge pipe 335.

[0047] The above description is only an illustrative specific implementation mode of the present invention and is not intended to limit the scope of the present invention. Any equivalent changes and modifications made by any technician in the field without departing from the concept and principle of the present invention should fall within the scope of protection of the present invention. It should also be noted that the various components of the present invention are not limited to the above-mentioned overall application. The various technical features described in the specification of the present invention can be selected one by one or multiple ones can be selected and used in combination according to actual needs. Therefore, the present invention should naturally cover other combinations and specific applications related to the invention point of this case.

Claims

1. A device for purifying glucose oxidase, comprising a mounting plate (1), and a vibration assembly (31) arranged above the mounting plate (1), characterized in that: The vibration assembly (31) comprises a fixing frame (311) fixedly connected to the bottom of the mounting plate (1); the inner wall of the other end of the fixing frame (311) is fixedly connected to a first motor (312); the output end of the first motor (312) is fixedly connected to a first output shaft (313); a driving gear (314) is fixedly sleeved on the top outer wall of the first output shaft (313); a driven gear (315) is meshed with the side wall of the driving gear (314); a transmission shaft (316) is fixedly connected to the inner wall of the driven gear (315); an L-shaped connecting plate (317) is rotatably connected to the middle outer wall of the transmission shaft (316) via a bearing; the other end of the L-shaped connecting plate (317) is fixedly connected to the bottom of the mounting plate (1); the top of the transmission shaft (316) is fixedly connected to a circular plate (31 8), the top of the circular plate (318) is fixedly connected with a first protrusion (319), the top of the first protrusion (319) is slidably connected with a second protrusion (3110), the top of the second protrusion (3110) is fixedly connected with a mixing barrel (3111), the outer wall of the mixing barrel (3111) is fixedly connected with a connecting block (3112), the inner wall of the connecting block (3112) is slidably connected with a limiting column (3113), the bottom of the limiting column (3113) is fixedly connected to the top of the mounting plate (1), the middle outer wall of the limiting column (3113) is fixedly connected with a rectangular block (3114), the top of the rectangular block (3114) is fixedly connected with a first spring (3115), and the other end of the first spring (3115) is fixedly connected to the bottom of the limiting column (3113).

2. A device for purifying glucose oxidase according to claim 1, characterized in that: A mixing assembly (32) is arranged inside the mixing barrel (3111), and the mixing assembly (32) comprises a second motor (322) fixedly connected to the top of the mixing barrel (3111), and an output end of the second motor (322) is fixedly connected to a second output shaft (323), and the second output shaft (323) movably passes through the top of the mixing barrel (3111) and extends to the inside of the mixing barrel (3111), and an outer wall of the second output shaft (323) is fixedly connected to a stirring blade (324), and an inner wall of the stirring blade (324) is rotatably connected to a rotating rod (325) via a bearing, and an outer wall of the rotating rod (325) is fixedly connected to a rotating plate (326).

3. A device for purifying glucose oxidase according to claim 2, characterized in that: The mixing assembly (32) further comprises a fixed frame (327) fixedly connected to the outer wall of the stirring blade (324); a displacement rod (328) is slidably connected to the inner wall of the side of the fixed frame (327); one end of the displacement rod (328) is fixedly connected to a limit block (329); a side wall of the limit block (329) is fixedly connected to a second spring (3210); the other end of the second spring (3210) is fixedly connected to the inner wall of the fixed frame (327); and the other end of the displacement rod (328) is fixedly connected to the side wall of the scraper (3211).

4. A device for purifying glucose oxidase according to claim 2, characterized in that: The mixing assembly (32) further comprises a feed port (321) connected to the top of the mixing barrel (3111).

5. The device for purifying glucose oxidase according to claim 1, characterized in that: The outer wall of the mixing barrel (3111) is provided with a filter assembly (33), and the filter assembly (33) includes a discharge hopper (331) connected to the outer wall of the mixing barrel (3111), a first valve (332) on the middle outer wall of the discharge hopper (331), and the other end of the discharge hopper (331) is connected to a filter box (333), the inner wall of the filter box (333) is fixedly connected with a filter plate (334), the outer wall of the filter box (333) is connected to a discharge pipe (335), and the outer wall of the discharge pipe (335) is fixedly connected with a second valve (336).

6. A device for purifying glucose oxidase according to claim 1, characterized in that: The bottom of the mounting plate (1) is fixedly connected with a support leg (2), the number of the support legs (2) is four, the four support legs (2) are equal in size, and the four support legs (2) are equidistantly fixedly connected to the four corners of the bottom of the mounting plate (1).

7. The device for purifying glucose oxidase according to claim 1, characterized in that: There are two connecting blocks (3112), the two connecting blocks (3112) are equal in size, and the two connecting blocks (3112) are symmetrically distributed along the central plane of the mixing barrel (3111).

8. A method for purifying glucose oxidase, applicable to a device for purifying glucose oxidase according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. First, glucose oxidase and diluent are put into the mixing barrel 3111 through the feed port 321; S2, secondly, the glucose oxidase and the diluent are stirred and mixed by the stirring blade 324 and the rotating plate 326; S3, then the second convex block 3110 is squeezed by the first convex block 319 to make the mixing barrel 3111 vibrate, thereby improving the mixing effect of the glucose oxidase and the diluent; S4. Finally, the mixed solution is filtered through the filter plate 334 to purify the mixed solution of glucose oxidase and the diluent.

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