Water injection bacterium inoculation device and inoculation method

Abstract

The invention discloses a water injection bacterium inoculation device and method. The water injection bacterium inoculation device comprises a sample disc, and sample cups, cleaning cups and bacterium culture bottles are circumferentially distributed on the sample disc; a telescopic rod is arranged on the sample disc, the telescopic rod can rotate in the circumferential direction of the sample disc, an injector is arranged on the telescopic rod, and the telescopic rod can drive the injector to move up and down. By means of rotation and stretching of the telescopic arm, the injector can move among different bottles, the injector does not need to be held by hands in the whole process, contact between the hands and liquid is avoided, operation safety is improved, corrosion damage of the liquid to the human body is reduced, and inoculation efficiency is improved.

Description

Technical Field

[0001] The invention belongs to the field of oilfield water treatment and relates to a water injection bacteria inoculation device and an inoculation method. Background Art

[0002] There are many bacteria in the oil field water injection system, which not only pose a hidden danger to the water quality, but also can cause strong corrosion and blockage to the water injection system, water injection pipelines and oil pipes, which will have different degrees of impact on the oil field water injection system. Therefore, the water injection bacteria content is a very important quality indicator, and accurate analysis of the water injection bacteria content is of great significance.

[0003] The existing operating method is to use a step-by-step dilution method for each sampling point, and use a syringe to manually inject the sample into the first-level saprophytic bacteria, sulfate-reducing bacteria, and iron bacteria culture bottles, and then extract the shaken liquid from the first-level three-category bacteria culture bottle and inject it into the second-level bacteria culture bottle; then extract the mixed liquid from the second-level three-category bacteria culture bottle and inject it into the third-level three-category bacteria culture bottle. Each sample requires eighteen bacteria culture bottles, and the syringe needs to be manually operated for eighteen injections. The operation is cumbersome and the work efficiency is low. Since the sample is alkaline and has a high salt content, it is inconvenient to use the syringe with gloves, and not wearing gloves will corrode the hands of the operator. Every time the operator goes out for sampling, the fingers of the operator are peeled off in many places. Summary of the invention

[0004] The purpose of the present invention is to solve the problem that when analyzing water injection bacteria in the prior art, manual operation is required for multiple times, the operation is cumbersome, the process is complicated, and when a person holds a syringe to operate, it is not only inconvenient, but also causes damage to the skin when the liquid contacts the human skin. A water injection bacteria inoculation device and inoculation method are provided. The whole process does not require the operation of a handheld syringe, which avoids the contact between human hands and liquid, improves the safety of operation, reduces the corrosive damage of liquid to the human body, and improves the inoculation efficiency.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A water-injected bacterial inoculation device comprises a sample tray, on which a sample cup, a cleaning cup and a bacterial culture bottle are circumferentially distributed;

[0007] The sample tray is provided with a telescopic rod, which can rotate along the circumference of the sample tray. A syringe is provided on the telescopic rod, and the telescopic rod can drive the syringe to move up and down.

[0008] A further improvement of the present invention is:

[0009] The sample tray is provided with a rotating shaft, and the telescopic rod is provided on the sample tray.

[0010] A connecting rod is arranged at the upper end of the telescopic rod, and the syringe is arranged on the connecting rod.

[0011] The connecting rod is provided with a connecting ring, and the syringe is fixed in the connecting ring.

[0012] The connecting rod is vertically connected to the telescopic rod.

[0013] The telescopic rod is connected to a motor, and the motor drives the telescopic rod to move up and down.

[0014] The sample plate is provided with a bacteria culture bottle hole, and the bacteria culture bottle is placed in the bacteria culture bottle hole.

[0015] There are eighteen bacterial culture bottles distributed in the circumference.

[0016] The diameters of the sample cup and the cleaning cup are larger than the diameter of the bacterial culture bottle.

[0017] A water injection bacteria inoculation method comprises the following steps:

[0018] Place the sample cup, cleaning cup and bacterial culture bottle on the sample tray in sequence;

[0019] Turn the telescopic rod to make the syringe and the sample cup correspond to each other up and down, adjust the telescopic rod to move downward, extend the syringe into the sample cup, and adjust the telescopic rod to move upward after sucking the sample;

[0020] Turn the telescopic rod to make the syringe correspond to the bacterial culture bottle up and down, adjust the telescopic rod to move downward, and inject the sample in the syringe into the bacterial culture bottle;

[0021] After each injection of the syringe, the telescopic rod is rotated to make the telescopic rod correspond vertically to the cleaning cup, and the telescopic rod is adjusted to move downward so that the syringe is extended into the cleaning cup for cleaning.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The invention discloses a water-injected bacterial inoculation device, comprising a sample tray capable of simultaneously placing a sample cup, a cleaning cup and a bacterial culture bottle, a rotatable telescopic rod being arranged on the sample tray, the telescopic rod being capable of driving a syringe to rotate along the sample tray, so that the syringe is vertically corresponding to the sample cup, the cleaning cup or the bacterial culture bottle, and the telescopic rod being capable of telescopically moving up and down, further driving the syringe to extend into any bottle to absorb or inject liquid, and realizing movement of the syringe between different bottles through rotation and extension of the telescopic arm, and no hand-held syringe operation is required throughout the whole process, thus avoiding contact between human hands and liquid, improving operation safety, reducing corrosion damage of liquid to human body, and improving inoculation efficiency.

[0024] Furthermore, in the present invention, the bacterial culture bottle is placed in the hole of the bacterial culture bottle, which is convenient for taking and fixing the bacterial culture bottle. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0026] Figure 1 It is the overall structure of the present invention.

[0027] Among them: 1- syringe; 2- push button switch; 3- motor; 4- connecting ring; 5- needle; 6- sample cup; 7- cleaning cup; 8- bacterial culture bottle; 9- supporting shaft; 10- bearing; 11- sample tray; 12- connecting rod; 13- telescopic rod. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments.

[0029] The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

[0030] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention as claimed but is merely representative of selected embodiments of the present invention.

[0031] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present invention.

[0032] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0033] In the description of the embodiments of the present invention, it should be noted that if the terms "upper", "lower", "horizontal", "inner", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, or is the orientation or position relationship in which the product of the invention is usually placed when used. It is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0034] Furthermore, the terms “first”, “second”, etc. are merely used for distinguishing descriptions and should not be understood as indicating or implying relative importance.

[0035] In addition, if the term "horizontal" appears, it does not mean that the component must be absolutely horizontal, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", which does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0036] In the description of the embodiments of the present invention, it is also necessary to explain that, unless otherwise clearly stipulated and limited, the terms "set", "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal connection of two elements.

[0037] For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0038] The present invention is further described in detail below in conjunction with the accompanying drawings:

[0039] See also Figure 1 The embodiment of the present invention discloses a water-injected bacterial inoculation device. 18 bacterial culture bottles 8, a sample cup, and a cleaning cup are placed on a sample tray. A telescopic rod 14 is used to replace hand-held injection. A 10ml stainless steel syringe is used to inoculate bottles one by one according to a pre-arranged sequence. The operator only needs to adjust the telescopic rod 13 without holding the syringe. This not only reduces direct contact with the skin, but also greatly improves the inoculation efficiency. The use of a rotatable bearing and a telescopic spring saves manpower. The present invention is small in size and easy to carry, and can be brought to any sampling point for rapid inoculation of samples.

[0040] The invention comprises: 1- a syringe 1; a button switch 2; a motor 3; a connecting ring 4; a needle 5; a sample cup 6; a cleaning cup 7; a bacterial culture bottle 8; a supporting shaft 9; a bearing 10; a sample tray 11; a connecting rod 12 and a telescopic rod 13.

[0041] Example 1

[0042] The present embodiment discloses a water injection bacterial inoculation device, comprising a sample tray 11, on which a sample cup 6, a cleaning cup 7 and a bacterial culture bottle 8 are circumferentially distributed; a telescopic rod 13 is arranged on the sample tray 11, and the telescopic rod 13 can rotate circumferentially along the sample tray 11, and a syringe 1 is arranged on the telescopic rod 13, and the telescopic rod 13 can drive the syringe 1 to move up and down.

[0043] In this embodiment, when the telescopic rod 13 rotates, the telescopic rod 13 can correspond vertically to the sample cup 6, the cleaning cup 7 or the bacterial culture bottle 8, and then when the telescopic rod 13 moves downward, the syringe 1 can extend into the bottle to inject or absorb the liquid.

[0044] Example 2

[0045] The embodiment discloses a water injection bacterial inoculation device, comprising a sample tray 11, on which a sample cup 6, a cleaning cup 7 and a bacterial culture bottle 8 are circumferentially distributed; a telescopic rod 13 is provided on the sample tray 11, and the telescopic rod 13 can rotate circumferentially along the sample tray 11, a syringe 1 is provided on the telescopic rod 13, and the telescopic rod 13 can drive the syringe 1 to move up and down, and a rotating shaft is provided on the sample tray 11, and the telescopic rod 13 is provided on the sample tray 11.

[0046] Furthermore, in the present embodiment, the rotating shaft comprises a supporting shaft 9 , a ball bearing 10 is sleeved on the outer side of the supporting shaft 9 , a sample tray 11 is sleeved on the outer side of the ball bearing 10 , and a telescopic rod 13 is connected to the supporting shaft 9 .

[0047] With the cooperation of the ball bearing, the support shaft 9 can drive the telescopic rod to rotate circumferentially along the sample plate 11 .

[0048] Example 3

[0049] The embodiment discloses a water injection bacterial inoculation device, comprising a sample tray 11, on which a sample cup 6, a cleaning cup 7 and a bacterial culture bottle 8 are circumferentially distributed; a telescopic rod 13 is provided on the sample tray 11, and the telescopic rod 13 can rotate circumferentially along the sample tray 11, and a syringe 1 is provided on the telescopic rod 13, and the telescopic rod 13 can drive the syringe 1 to move up and down, and a connecting rod 12 is provided at the upper end of the telescopic rod 13, and the syringe 1 is arranged on the connecting rod 12.

[0050] Example 4

[0051] The embodiment discloses a water-injected bacterial inoculation device, including a sample tray 11, on which a sample cup 6, a cleaning cup 7 and a bacterial culture bottle 8 are circumferentially distributed; a telescopic rod 13 is provided on the sample tray 11, and the telescopic rod 13 can rotate circumferentially along the sample tray 11, a syringe 1 is provided on the telescopic rod 13, and the telescopic rod 13 can drive the syringe 1 to move up and down, and a connecting rod 12 is provided at the upper end of the telescopic rod 13, and the syringe 1 is provided on the connecting rod 12. A connecting ring 4 is provided on the connecting rod 12, and the syringe 1 is fixed in the connecting ring 4.

[0052] Furthermore, the connecting rod 12 is fixed to the telescopic end of the telescopic rod 13 .

[0053] Example 5

[0054] The embodiment discloses a water-injected bacterial inoculation device, including a sample tray 11, on which a sample cup 6, a cleaning cup 7 and a bacterial culture bottle 8 are circumferentially distributed; a telescopic rod 13 is provided on the sample tray 11, and the telescopic rod 13 can rotate circumferentially along the sample tray 11, and a syringe 1 is provided on the telescopic rod 13, and the telescopic rod 13 can drive the syringe 1 to move up and down, and a connecting rod 12 is provided at the upper end of the telescopic rod 13, and the syringe 1 is provided on the connecting rod 12. A connecting ring 4 is provided on the connecting rod 12, and the syringe 1 is fixed in the connecting ring 4, and the connecting rod 12 is vertically connected to the telescopic rod 13.

[0055] The connecting rod 12 is a horizontal rod, one end of which is fixedly connected to the telescopic rod 13, and the other end is provided with a connecting ring 4, which facilitates the taking and fixing of the syringe and the replacement of the syringe 1 during the vaccination process.

[0056] Furthermore, the connecting rod 12 allows a certain distance between the syringe 1 and the sample cup 6 , the cleaning cup 7 or the bacterial culture bottle 8 to avoid interference between them during the inoculation process.

[0057] Example 6

[0058] The embodiment discloses a water injection bacterial inoculation device, including a sample tray 11, on which a sample cup 6, a cleaning cup 7 and a bacterial culture bottle 8 are circumferentially distributed; a telescopic rod 13 is provided on the sample tray 11, and the telescopic rod 13 can rotate circumferentially along the sample tray 11, and a syringe 1 is provided on the telescopic rod 13, and the telescopic rod 13 can drive the syringe 1 to move up and down, and the telescopic rod 13 is connected to a motor 3, and the motor 3 drives the telescopic rod 13 to move up and down.

[0059] In this embodiment, the telescopic rod 13 is an electric telescopic rod and is driven by a motor 3. A button switch 2 is connected to the telescopic rod 13. The switch 2 cooperates with the motor 3 to open and close the telescopic rod 14, driving the telescopic rod 13 to move upward or downward.

[0060] The structure of the telescopic rod 13 in this embodiment is a threaded rotating telescopic rod. This embodiment is not limited to a telescopic rod of this structure. As long as the telescopic rod 13 can be telescopically moved upward or downward, it is not limited to electric. As long as the telescopic rod 13 can be extended upward or moved downward, it can be used in the prior art.

[0061] Example 7

[0062] The embodiment discloses a water injection bacterial inoculation device, including a sample tray 11, on which a sample cup 6, a cleaning cup 7 and a bacterial culture bottle 8 are circumferentially distributed; a telescopic rod 13 is provided on the sample tray 11, and the telescopic rod 13 can rotate circumferentially along the sample tray 11, a syringe 1 is provided on the telescopic rod 13, and the telescopic rod 13 can drive the syringe 1 to move up and down, the telescopic rod 13 is connected to a motor 3, and the motor 3 drives the telescopic rod 13 to move up and down, and a bacterial culture bottle hole is opened on the sample tray 11, and the bacterial culture bottle 8 is placed in the bacterial culture bottle hole.

[0063] The bacterial culture bottle 8 is stuck in the hole of the bacterial culture bottle, which is convenient for quick taking and placement of the bacterial culture bottle 8 .

[0064] Example 8

[0065] The embodiment discloses a water injection bacterial inoculation device, including a sample tray 11, on which a sample cup 6, a cleaning cup 7 and a bacterial culture bottle 8 are circumferentially distributed; a telescopic rod 13 is provided on the sample tray 11, and the telescopic rod 13 can rotate circumferentially along the sample tray 11, a syringe 1 is provided on the telescopic rod 13, and the telescopic rod 13 can drive the syringe 1 to move up and down, the telescopic rod 13 is connected to a motor 3, and the motor 3 drives the telescopic rod 13 to move up and down, and a bacterial culture bottle hole is opened on the sample tray 11, and the bacterial culture bottle 8 is placed in the bacterial culture bottle hole, and there are eighteen bacterial culture bottles 8 distributed circumferentially.

[0066] The number of the bacterial culture bottles 8 can be set according to the number of bacterial culture bottles 8 required for different bacterial analyses.

[0067] Example 9

[0068] The embodiment discloses a water injection bacterial inoculation device, including a sample tray 11, on which a sample cup 6, a cleaning cup 7 and a bacterial culture bottle 8 are circumferentially distributed; a telescopic rod 13 is provided on the sample tray 11, and the telescopic rod 13 can rotate circumferentially along the sample tray 11, a syringe 1 is provided on the telescopic rod 13, and the telescopic rod 13 can drive the syringe 1 to move up and down, the telescopic rod 13 is connected to a motor 3, and the motor 3 drives the telescopic rod 13 to move up and down, a bacterial culture bottle hole is opened on the sample tray 11, and the bacterial culture bottle 8 is placed in the bacterial culture bottle hole, and there are eighteen bacterial culture bottles 8 distributed circumferentially, and the diameters of the sample cup 6 and the cleaning cup 7 are larger than the diameter of the bacterial culture bottle 8.

[0069] Furthermore, one sample cup 6 and one cleaning cup 7 are provided.

[0070] This embodiment also discloses a water injection bacteria inoculation method, comprising the following steps:

[0071] Place 18 bacterial culture bottles 8 in the holes of the bacterial culture bottles one by one according to their numbers, pry open the protective covers of the bottle mouths with tweezers, and place iron bacteria, planktonic bacteria, and sulfate-reducing bacteria in different bacterial culture bottles 8 respectively;

[0072] Place the sample cup 6 and the cleaning cup 7 in the designated area;

[0073] Pour the sample into the sample cup 6 and pour ultrapure water into the cleaning cup 7;

[0074] Place the syringe 1 in the connecting ring 4, and install the needle 5 at the lower part of the syringe 1;

[0075] Rotate the telescopic rod 13 so that the telescopic rod 13 corresponds to the sample cup 6 up and down, then press the button switch 2, the motor 3 starts, and the motor 3 drives the telescopic rod 13 to rotate and move downward. At this time, the needle 5 extends into the sample cup 6 to inhale 10mL of sample;

[0076] After the suction is completed, the motor 3 is started again to rotate the telescopic rod 13 upward until the needle 5 is separated from the sample cup 6;

[0077] The telescopic rod 13 is rotated again to inject 1.0 mL of sample into the six primary bacterial culture bottles respectively;

[0078] In this step, the six primary bacterial culture bottles include two iron bacteria culture bottles, two planktonic bacteria culture bottles, and two sulfate-reducing bacteria culture bottles;

[0079] After the injection is completed, the telescopic rod 13 is rotated so that the needle head corresponds to the cleaning cup 7, and then the telescopic rod 13 drives the needle head 5 to extend into the cleaning cup 7 for cleaning;

[0080] After cleaning, replace syringe 1, and then extract 1.0 mL of liquid from the primary iron bacteria culture bottle, the planktonic bacteria culture bottle, and the sulfate-reducing bacteria culture bottle and inject it into the secondary bacteria culture bottle corresponding to each bacterium;

[0081] In this step, after each type of bacteria is injected, the syringe 1 is replaced;

[0082] Then, 1.0 mL of liquid was drawn from the secondary iron bacteria culture bottle, the planktonic bacteria culture bottle, and the sulfate-reducing bacteria culture bottle respectively and injected into the tertiary bacteria culture bottle corresponding to each bacterium.

[0083] Transfer the 18 inoculated bacterial culture bottles into a constant temperature box for bacterial culture;

[0084] Clean and sterilize the syringe, and clean the sample cup 6 and the cleaning cup 7.

[0085] In this embodiment, when performing secondary and tertiary liquid injection, the rotation and up and down movement of the syringe 1 are both completed by the telescopic rod 13 .

[0086] In this embodiment, a sample tray is used as a carrier to place the sample cup 6, the cleaning cup 7 and the bacterial culture bottle 8 respectively thereon, and the circumferential distribution and the circumferential rotation of the telescopic rod 13 at a later stage facilitate the upper and lower correspondence of different bottles with the syringe 1. In this embodiment, it is not necessary for a person to hold the syringe and the bacterial culture bottle 8 for operation, thus avoiding the risk of contact between the hands and the liquid due to improper operation, which may cause the liquid to corrode and damage the human skin.

[0087] In this embodiment, the bacterial culture bottles 8 required for the test can be placed in advance on the sample tray 11 in order, and inoculation can be achieved bottle by bottle by rotating the telescopic rod 13. The inoculation efficiency is high, the operation process is relatively simple, and during the inoculation process, only the syringe needs to be replaced.

[0088] The device has a simple structure and is easy to carry. It can be taken to any sampling point for rapid inoculation of samples, and has higher flexibility in application scenarios.

[0089] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A water injection bacteria inoculation device, characterized in that: The sample tray (11) comprises a sample cup (6), a cleaning cup (7) and a bacterial culture bottle (8) which are circumferentially distributed on the sample tray (11); The sample tray (11) is provided with a telescopic rod (13), the telescopic rod (13) can rotate along the circumference of the sample tray (11), a syringe (1) is provided on the telescopic rod (13), and the retractable rod (14) can drive the syringe (1) to move up and down.

2. A water injection bacteria inoculation device according to claim 1, characterized in that: The sample plate (11) is provided with a rotating shaft, and the telescopic rod (13) is provided on the sample plate (11).

3. A water injection bacteria inoculation device according to claim 1, characterized in that: A connecting rod (12) is arranged at the upper end of the telescopic rod (13), and the syringe (1) is arranged on the connecting rod (12).

4. A water injection bacteria inoculation device according to claim 3, characterized in that: The connecting rod (12) is provided with a connecting ring (4), and the syringe (1) is fixed in the connecting ring (4).

5. A water injection bacteria inoculation device according to claim 4, characterized in that: The connecting rod (12) is vertically connected to the telescopic rod (13).

6. A water injection bacteria inoculation device according to claim 1, characterized in that: The telescopic rod (13) is connected to the motor (3), and the motor (3) drives the telescopic rod (13) to move up and down.

7. A water injection bacteria inoculation device according to claim 1, characterized in that: The sample plate (11) is provided with a bacteria culture bottle hole, and the bacteria culture bottle (8) is placed in the bacteria culture bottle hole.

8. A water injection bacteria inoculation device according to claim 7, characterized in that: There are eighteen bacterial culture bottles (8) distributed in the circumference.

9. A water injection bacteria inoculation device according to claim 7, characterized in that: The diameters of the sample cup (6) and the cleaning cup (7) are greater than the diameter of the bacterial culture bottle (8).

10. A water injection bacterial inoculation method, characterized in that: The following steps are involved: Placing the sample cup (6), the cleaning cup (7) and the bacterial culture bottle (8) on the sample tray (11) in sequence; The telescopic rod (13) is rotated to make the syringe correspond to the sample cup (6) up and down, and the telescopic rod (13) is adjusted to move downward to make the syringe (1) extend into the sample cup (6), and after the sample is sucked, the telescopic rod (13) is adjusted to move upward; The telescopic rod (13) is rotated to make the syringe correspond to the bacterial culture bottle (8) up and down, and the telescopic rod (13) is adjusted to move downward to inject the sample in the syringe (1) into the bacterial culture bottle (8); After the syringe completes each injection, the telescopic rod (13) is rotated to make the telescopic rod (13) correspond vertically to the cleaning cup (7), and the telescopic rod (13) is adjusted to move downward so that the syringe (1) extends into the cleaning cup (7) for cleaning.

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