Kit and buffer solution box integrated device for gene sequencer and gene sequencer

Abstract

The invention provides a kit and buffer solution box integrated device for a gene sequencer and the gene sequencer, and relates to the technical field of gene sequencing, the kit and buffer solution box integrated device comprises a kit and a buffer solution box, and a box body of the kit is provided with a buffer solution box accommodating part; a detachable first identification piece is arranged on the side, close to the containing part, in the kit, the first identification piece is provided with a first identification part, and the first identification part comprises a plurality of first identification areas used for identifying information of the kit; the buffer solution box is provided with a detachable second identification piece, the second identification piece is provided with a second identification part, and the second identification part comprises a plurality of second identification areas used for identifying information of the buffer solution box; a first fool-proof part and a second fool-proof part are respectively arranged on the first identification piece and the second identification piece; the first fool-proof part and the second fool-proof part are matched with each other, so that the buffer solution box is matched with the kit. The technical problem that in the prior art, an independent kit and a buffer solution box affect the overall size of the gene sequencer is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of gene sequencing, and in particular to a reagent kit and a buffer box integrated device for a gene sequencer and a gene sequencer. Background Art

[0002] With the development of science and technology, instruments are moving towards intelligence, miniaturization and portability. As an important biotechnology instrument, the miniaturization and integration of gene sequencers has become one of the important development directions. As important structural components of gene sequencers, the size design and integrated layout of reagent kits and buffer boxes directly affect the miniaturization of the entire gene sequencer. Summary of the invention

[0003] The present invention provides a reagent kit and buffer box integrated device for a gene sequencer and a gene sequencer to solve the technical problem that the independent reagent kit and buffer box in the prior art affect the overall size of the gene sequencer. The preferred technical solutions among the many technical solutions provided by the present invention can produce many technical effects as described below.

[0004] To achieve the above object, on the one hand, the present invention provides a reagent kit and a buffer box integrated device for a gene sequencer, comprising a reagent kit and a buffer box, wherein:

[0005] The box body of the reagent kit is provided with a receiving portion for receiving the buffer box;

[0006] A detachable first identification member is provided on one side of the reagent kit near the containing portion, the first identification member is provided with a first identification portion, and the first identification portion includes a plurality of first identification areas for identifying information of the reagent kit;

[0007] The buffer box is provided with a detachable second identification piece, the second identification piece is provided with a second identification portion, and the second identification portion includes a plurality of second identification areas for identifying information of the buffer box;

[0008] The first identification member and the second identification member are also provided with a first fool-proofing portion and a second fool-proofing portion, respectively. The first fool-proofing portion and the second fool-proofing portion cooperate with each other to match the buffer box and the reagent kit.

[0009] Optionally, the first fool-proofing portion includes a plurality of first fool-proofing structures, and the second fool-proofing portion includes a plurality of second fool-proofing structures, and each of the first fool-proofing structures is matched with a corresponding second fool-proofing structure.

[0010] Optionally, each of the first fool-proofing structures is matched with a corresponding second fool-proofing structure, including the matching of the position and shape of each of the first fool-proofing structures with a corresponding second fool-proofing structure.

[0011] Optionally, the first identification part and the second identification part are both configured as turntable structures, the rotation center axis of the first identification part is arranged along a direction parallel to the long side of the reagent box, and the rotation center axis of the second identification part is arranged along a direction perpendicular to the bottom surface of the buffer box.

[0012] Optionally, the first identification area is arranged on a side of a turntable of a turntable structure of the first identification portion, and the first fool-proofing portion is vertically arranged on a bottom surface of the turntable.

[0013] Optionally, the first fool-proofing part is also a turntable structure, and the first fool-proofing structure is arranged on a side of the turntable structure of the first fool-proofing part.

[0014] Optionally, the first identification portion and / or the first anti-foolproof portion is a regular polygonal cylinder, and a first identification cavity is provided on the reagent kit, and the shape of the first identification cavity is adapted to the shape of the first identification portion and / or the first anti-foolproof portion so that the first identification part cannot rotate after being installed in the first identification cavity.

[0015] Optionally, the second identification area is arranged in a peripheral area of ​​an upper surface of a turntable of a turntable structure of the second identification portion, and the second fool-proofing portion is arranged on a lower surface of the turntable.

[0016] Optionally, the second identification portion is a regular polygonal cylinder, and a second identification cavity is provided on the buffer box, and the shape of the second identification cavity is adapted to the shape of the second identification portion so that the second identification member cannot rotate after being installed in the second identification cavity.

[0017] Optionally, the first fool-proofing structure is a clamping groove arranged on each side surface of the regular polygonal column of the first fool-proofing part, and the second fool-proofing structure is a clamping column arranged on the second fool-proofing part and correspondingly adapted to the first fool-proofing structure.

[0018] Optionally, the test kit includes a first identification box, the first identification cavity is arranged in the first identification box, the first identification cavity includes a first cavity for accommodating the first identification part and a second cavity for accommodating the first anti-foolproof part, a first rotating shaft is arranged at the bottom center of the first anti-foolproof part, and a rotating shaft groove is arranged on the side of the first identification box, and when the first identification part is installed in the first identification box, the first rotating shaft is clamped in the rotating shaft groove.

[0019] Optionally, the buffer box includes a second identification box, the second identification cavity is a cavity opened downward in the second identification box, a second rotating shaft is arranged in the middle of the upper surface of the turntable of the second identification member, and a rotating shaft hole is arranged in the middle position of the top of the second identification box, and when the turntable of the second identification member is installed in the second identification box, the second rotating shaft is clamped into the rotating shaft hole.

[0020] Optionally, the reagent kit is further provided with a reagent kit cover, and a first observation port is provided on the reagent kit cover corresponding to the first identification area.

[0021] Optionally, the second identification box is provided with an observation hole corresponding to the second identification area, and the buffer solution box is further provided with a buffer solution box cover, and the buffer solution box cover is provided with a second observation port corresponding to the observation hole.

[0022] On the other hand, the present invention provides a gene sequencer, comprising a reagent kit and a buffer box integrated device for a gene sequencer as described above, wherein the buffer box is arranged in a buffer box accommodating portion within the reagent kit box body, the markings on the relative positions of the first identification part and the second identification part correspond to each other, and the first anti-foolproofing portion and the second anti-foolproofing portion are adapted to each other.

[0023] The technical solution of the present invention may include the following beneficial effects:

[0024] The present invention provides an integrated device of a reagent kit and a buffer box for a gene sequencer and a gene sequencer, wherein the integrated device of a reagent kit and a buffer box for a gene sequencer integrates the buffer box into the reagent kit box body by providing a receiving portion in the reagent kit box body, thereby realizing the disassembly and assembly of the reagent kit and the buffer box, which not only meets the differentiated requirements of the reagent storage environment but also meets the requirements of miniature integration; in addition, by providing a first identification piece on the reagent kit and a second identification piece on the buffer box, when the first identification area and the second identification area correspond, the first fool-proofing part thereon matches the second fool-proofing part, thereby solving the problem that it is difficult to achieve one-to-one corresponding assembly when the reagent kit and the buffer box are of different types. It can be seen that the integrated device of a reagent kit and a buffer box for a gene sequencer of this scheme solves the technical problem in the prior art that the independent reagent kit and buffer box affect the overall size of the gene sequencer. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 It is a schematic structural diagram of a kit and a buffer box integrated device for a gene sequencer in a disassembled state according to an embodiment of the present invention;

[0027] Figure 2 is a top view of an assembled state of a reagent kit and a buffer box integrated device for a gene sequencer according to an embodiment of the present invention;

[0028] Figure 3 It is a schematic structural diagram of a reagent kit in a reagent kit and a buffer box integrated device for a gene sequencer according to an embodiment of the present invention;

[0029] Figure 4 It is a schematic diagram of the structure of a buffer box in a reagent kit and buffer box integrated device for a gene sequencer according to an embodiment of the present invention;

[0030] Figure 5 It is a schematic structural diagram of the first identification member and the second identification member in the integrated device of the reagent kit and the buffer box for a gene sequencer according to an embodiment of the present invention, which are engaged with each other;

[0031] Figure 6 It is a schematic diagram of the application structure of reagents and buffer in the gene sequencer of an embodiment of the present invention.

[0032] In the figure: 1. reagent box; 11. reagent box cover; 111; first observation port; 112; first buckle; 12. first identification piece; 121. first rotating shaft; 122. first identification area; 123. first fool-proof structure; 13. reagent box body; 131. rotating shaft groove; 132. first identification cavity; 2. buffer box; 21. buffer box cover; 211. second observation port; 212. second buckle; 22. second identification piece; 221. second rotating shaft; 222. second identification area; 223. second fool-proof structure; 23. buffer box body; 231. rotating shaft hole; 232. second identification box; 233. observation hole; 10. sequencing chip; 20. chip platform. DETAILED DESCRIPTION

[0033] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0034] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element at the same time. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or there may be an intermediate element at the same time.

[0035] like Figure 6 As shown, during the gene sequencing process of the gene sequencer, the reagent kit is configured to store one or more reagents and corresponds to a certain filling volume. The reagents in the reagent kit are controllably transported to the sequencing chip 10 on the chip platform 20 through the flow diversion system, so as to perform amplification reactions and sequencing reactions in the sequencing chip 10; the buffer box is configured to store corresponding doses of buffer corresponding to different reagent quantities, so that after the sequencing reaction, the reagents can be controllably transported to the sequencing chip 10 through the flow diversion system to rinse the sequencing chip 10.

[0036] The reagent kit and buffer box are mostly designed as two independent boxes. When working, the two boxes are placed in the instrument and correspond to the two mechanism modules in the instrument respectively, resulting in low instrument space utilization. If the reagent kit and buffer box are integrated into one and correspond to only one mechanism module of the instrument, the instrument space utilization will be significantly improved. There are two main reasons why the reagent kit and buffer box are currently two independent boxes: first, due to different gene sequencing projects of different customers, the reagent kit and buffer will produce a variety of filling reagent products to meet the different needs of customers; second, the storage environment requirements of the reagents filled in the two boxes of the reagent kit and buffer box are different. The reagents in the reagent kit need to be stored at low temperature, and the reagents in the buffer box need to be stored at room temperature.

[0037] In view of the above two reasons, the reagent kit and buffer box are integrated into one: first, the reagent kit and buffer box need to be designed as a disassembled and assembled structure. When disassembled, they can meet the requirements of different storage environments, and when assembled, they can meet the requirements of miniaturized instrument layout. Secondly, when assembling the reagent kit and buffer box, a reliable fool-proof structure is required to ensure that the two boxes with the same amount of reagents are assembled one by one. Incorrect assembly will lead to sequencing failure.

[0038] like Figures 1 to 5 As shown, the present invention provides a reagent kit and buffer box integrated device for a gene sequencer, comprising a reagent kit 1 and a buffer box 2, wherein:

[0039] The box body 13 of the reagent kit 1 is provided with a receiving portion for receiving the buffer box 2;

[0040] A detachable first identification member 12 is provided on one side of the reagent box 1 close to the containing portion. The first identification member 12 is provided with a first identification portion. The first identification portion includes a plurality of first identification areas 122 for identifying information of the reagent box 1.

[0041] The buffer box 2 is provided with a detachable second identification member 22, the second identification member 22 is provided with a second identification portion, and the second identification portion includes a plurality of second identification areas 222 for identifying information of the buffer box 2;

[0042] The first identification member 12 and the second identification member 22 are also provided with a first foolproof portion and a second foolproof portion, respectively. The first foolproof portion and the second foolproof portion cooperate with each other to match the buffer box 2 and the reagent 1.

[0043] Specifically, the reagent kit and buffer box integrated device for gene sequencer integrates the buffer box 2 into the reagent kit box body 13 by setting a receiving portion on the reagent kit box body 13, thereby realizing the disassembly and assembly of the reagent kit 1 and the buffer box 2, which not only meets the differentiated requirements of the reagent storage environment but also meets the requirements of micro-integration; in addition, by setting the first identification piece 12 on the reagent kit 1 and the second identification piece 22 on the buffer box 2, only when the first identification area 122 and the second identification area 222 correspond to each other, the first foolproof part thereon matches the second foolproof part, thereby solving the problem that it is difficult to achieve one-to-one corresponding assembly when there are multiple types of reagent kits 1 and buffer boxes 2. The first identification area 122 and the second identification area 222 can be used to mark different information such as the capacity, specification and type of the reagents in the reagent kit 1 and the buffer box 2. For example, the first identification area is provided with six identifications such as A, B, C, D, E, and F according to the six filling volumes of the reagent kit 1. The identifications are not limited to letters, but may also be numbers or other symbols. The first fool-proofing part is provided with six fool-proofing structures of different shapes accordingly; the second identification area of ​​the buffer solution box 2 is provided with six identifications A, B, C, D, E, and F with the same symbols as those of the first identification area according to the six buffer solution filling volumes of the corresponding reagent volumes. The corresponding second fool-proofing part is six fool-proofing structures of different shapes. The first fool-proofing structure and the second fool-proofing structure can be mutually snapped and matched when and only when the identifications of the first identification area correspond to those of the second identification area.

[0044] As an optional embodiment, the first fool-proofing portion includes a plurality of first fool-proofing structures 123 , and the second fool-proofing portion includes a plurality of second fool-proofing structures 223 , and each of the first fool-proofing structures 123 is matched with a corresponding second fool-proofing structure 223 .

[0045] As an optional implementation, each of the first fool-proofing structures 123 is matched with a corresponding second fool-proofing structure 223 , including the matching of the position and shape of each of the first fool-proofing structures 123 and a corresponding second fool-proofing structure 223 .

[0046] Specifically, each first foolproof structure 123 is adapted to a corresponding second foolproof structure 223, including the position adaptation of the first foolproof structure 123 and the corresponding second foolproof structure 223, such as when the buffer box 2 is integrated into the reagent box body 13, the position of the first foolproof structure 123 and the corresponding second foolproof structure 223 are exactly corresponding, and the assembly will not be impossible due to position misalignment; it also includes the structural adaptation of the first foolproof structure 123 and the corresponding second foolproof structure 223, such as Figure 5 One of the first foolproof structures 123 shown is a circular hole or a triangle, and the corresponding second foolproof structure 223 is also a cylinder or a triangular prism of the same size and shape. In other words, the buffer box 2 and the reagent 1 can be foolproofed only by the position of each of the first foolproof structures 123 and a corresponding second foolproof structure 223, or by the shape of each of the first foolproof structures 123 and a corresponding second foolproof structure 223, or by both the position and shape of each of the first foolproof structures 123 and a corresponding second foolproof structure 223.

[0047] As an optional embodiment, the first identification part and the second identification part are both configured as a turntable structure, the rotation center axis of the first identification part is arranged along a direction parallel to the long side of the reagent box 1, and the rotation center axis of the second identification part is arranged along a direction perpendicular to the bottom surface of the buffer box 2, so as to facilitate the installation and matching of the first identification part and the second identification part.

[0048] As an optional embodiment, the first identification area is arranged on the side of the turntable of the turntable structure of the first identification part, and the first fool-proof part is arranged vertically on the bottom surface of the turntable. The first fool-proof part and the first identification part are arranged integrally and can rotate synchronously, so as to synchronously adjust the first identification area and the first fool-proof part according to the filling information of the reagent box.

[0049] As an optional embodiment, the first foolproofing part is also a rotating disk structure, and the first foolproofing structure 123 is arranged on the side of the rotating disk structure of the first foolproofing part. In this way, the structures of the first foolproofing part and the first foolproofing structure 123 are simple, and it is convenient to adjust different first foolproofing structures 123 to match with the second foolproofing structure 223.

[0050] As an optional embodiment, the first identification portion and / or the first foolproof portion is a regular polygonal column, and the reagent box 1 is provided with a first identification cavity 132, the shape of which is adapted to the shape of the first identification portion and / or the first foolproof portion, so that the first identification member 12 cannot rotate after being installed in the first identification cavity. The first identification cavity 132 is provided to facilitate the removal and installation positioning of the first identification member 12.

[0051] As an optional embodiment, the second identification area 222 is arranged in the peripheral area of ​​the upper surface of the turntable of the turntable structure of the second identification part, and the second fool-proofing part is arranged on the lower surface of the turntable.

[0052] As an optional embodiment, the second identification portion is a regular polygonal cylinder, and a second identification cavity is provided on the buffer box 2, and the shape of the second identification cavity is adapted to the shape of the second identification portion so that the second identification member cannot rotate after being installed in the second identification cavity.

[0053] Specifically, the central axis of the rotating shaft of the first identification member 12 is set along the long side direction of the reagent box 1, the first identification area 122 is on the side of the turntable, and the first fool-proofing structure 123 is set on the side of the regular polygonal cylinder; the corresponding central axis of the rotating shaft of the second identification member 22 is set in a direction perpendicular to the bottom surface of the buffer box 2, and the second identification area 222 is set on the upper surface of the turntable; when the buffer box 2 is assembled with the reagent box 1, the second identification area 222 on the buffer box 2 corresponds to the first identification area 122 on the reagent box 1, the buffer box 2 is placed downward into the accommodating portion of the reagent box 1, and the second fool-proofing structure 223 can also be snap-fitted with the first fool-proofing structure 123 from top to bottom.

[0054] The first identification cavity 132 and the second identification cavity are respectively arranged on the reagent box 1 and the buffer box 2 corresponding to the first identification part 12 and the second identification part 22, and the shapes of the first identification cavity 132 and the second identification cavity are respectively adapted to the first identification part 12 and the second identification part 22, thereby ensuring that the first identification part 12 and the second identification part 22 are easy to install and position when installing after disassembly, thereby providing protection and convenience for the disassembly and installation of the first identification part 12 and the second identification part 22.

[0055] As an optional embodiment, the first fool-proofing structure 123 is a snap-in groove arranged on each side of the regular polygonal column of the first fool-proofing part, and the second fool-proofing structure 223 is a snap-in column arranged on the second fool-proofing part and correspondingly adapted to the first fool-proofing structure 123.

[0056] Specifically, for example, the first fool-proofing structure 123 is configured as six different shapes of snap-fit ​​grooves arranged on each side of a regular hexagonal column, and the second fool-proofing structure 223 is configured as six different shapes of snap-fit ​​columns corresponding to the six shapes of snap-fit ​​grooves.

[0057] As an optional embodiment, the test kit 1 includes a first identification box, the first identification cavity 132 is arranged in the first identification box, the first identification cavity 132 includes a first cavity for accommodating the first identification part and a second cavity for accommodating the first anti-foolproof part, a first rotating shaft 121 is arranged at the bottom center of the first anti-foolproof part, and a rotating shaft groove 131 is arranged on the side of the first identification box, when the first identification part 12 is installed in the first identification box, the first rotating shaft 121 is clamped in the rotating shaft groove 131.

[0058] Specifically, the first identification member 12 is detachably connected to the reagent box 1 through the first identification box, so that when the reagent box 1 is filled with different reagent amounts, the position of the first identification member 12 can be adjusted to turn the first identification area 122 corresponding to the reagent amount to a visible position. The first identification area 122 is accommodated in the first cavity, and the shape of the inner surface of the bottom of the second cavity corresponds to the outer surface shape of the regular polygonal cylinder of the first foolproof part. After the first identification member 12 is assembled to the reagent box 1, the structure of the second cavity can support and limit the first identification member 12. Among them, the first cavity is close to one side of the reagent box 1, and the second cavity protrudes on one side of the buffer box accommodating part in the reagent box and protrudes in the buffer box accommodating part.

[0059] As an optional embodiment, the buffer box 2 includes a second identification box 232, the second identification cavity is a cavity opened downward in the second identification box 232, a second rotating shaft 221 is set in the middle of the upper surface of the turntable of the second identification member 22, and a rotating shaft hole 231 is set in the middle position of the top of the second identification box 232. When the turntable of the second identification member 22 is installed in the second identification box 232, the second rotating shaft 221 is snapped into the rotating shaft hole 231.

[0060] Specifically, the second identification member 22 is detachably connected to the buffer box 2 via the second identification box 232, so that when the buffer box 2 is filled with different buffer doses, the position of the second identification member 22 can be adjusted to turn the second identification area 222 corresponding to the buffer dose to a visible position. The second identification member 22 is inserted into the cavity of the second identification box 232 from bottom to top, and the second rotating shaft 221 is snapped into 231. The shape of the cavity corresponds to the shape of the turntable of the second identification member 22. After assembly, the cavity structure can support and limit the second identification member 22.

[0061] As an optional embodiment, the reagent kit 1 is further provided with a reagent kit cover 11 , and a first observation port 111 is provided on the reagent kit cover 11 corresponding to the first identification area of ​​the first identification element.

[0062] Specifically, after the reagent kit 1 is filled, according to the amount of reagent filled in the reagent kit 1, the first identification area 122 corresponding to the amount of reagent is turned to face upward, that is, upward in a direction perpendicular to the bottom of the reagent kit 1, and then the first identification part, the first anti-mistake part and the first rotating shaft of the first identification member 12 are installed in place corresponding to the first cavity, the second cavity and the rotating shaft groove, and the reagent kit cover 11 is assembled with the reagent kit box body 13 through the first buckle 112. The upward first identification area 122 on the first identification member 12 can be read by the user through the first observation port 111 on the reagent kit cover 11 to identify the current reagent kit filling amount information.

[0063] As an optional embodiment, the second identification box 232 is provided with an observation hole 233 corresponding to the second identification area, and the buffer box 2 is further provided with a buffer box cover 21 , and the buffer box cover 21 is provided with a second observation port 211 corresponding to the observation hole 233 .

[0064] Specifically, after the buffer box 2 is filled, first, according to the filling amount of the buffer in the buffer box 2, the second identification area 222 corresponding to the buffer amount is rotated to a visible position that can be observed through the observation hole 233 and the second observation port 211, and then the first identification member 22 is assembled from bottom to top into the cavity of the second identification box 232, and the second rotating shaft 221 is engaged with the rotating shaft 231 hole to be assembled and fixed, and finally the buffer box cover 21 is engaged and assembled and fixed with the buffer box body 23 through the second buckle 212. The second identification area can be read by the user through the second observation port 211 to identify the current reagent kit filling amount information.

[0065] Due to the differences in the storage environment requirements for reagents and buffer solutions, the assembled reagent kit 1 and buffer solution box 2 are packaged, transported and stored as independent products; when sequencing is required, the user will select the reagent kit 1 and buffer solution box 2 with corresponding filling volumes according to different gene sequencing projects and assemble them into one through the first identification piece 12 and the second identification piece 22. Due to the turntable's fool-proof structure, only two boxes with the same identification can be assembled, which can effectively avoid the problem of sequencing test failure caused by incorrect assembly of boxes with different filling volume identifications.

[0066] On the other hand, the present invention provides a gene sequencer, comprising a reagent kit and buffer box integrated device for a gene sequencer as described above, wherein the buffer box 2 is arranged in a buffer box accommodating portion within the reagent kit body 11, the first identification member and the second identification member have corresponding markings on relative positions, and the first anti-foolproofing portion and the second anti-foolproofing portion are adapted to each other.

[0067] Specifically, after the buffer box 2 is installed correspondingly to the reagent kit 1, a reagent kit and a buffer box integration device for a gene sequencer is obtained, achieving micro-integration, and the whole device is applied to the gene sequencer to meet the miniaturization requirements of the gene sequencer.

[0068] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0069] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0070] The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art who is familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A kit and buffer box integrated device for a gene sequencer, characterized in that: It includes a reagent box and a buffer box, wherein: The box body of the reagent kit is provided with a receiving portion for receiving the buffer box; A detachable first identification member is provided on one side of the reagent kit near the containing portion, the first identification member is provided with a first identification portion, and the first identification portion includes a plurality of first identification areas for identifying information of the reagent kit; The buffer box is provided with a detachable second identification piece, the second identification piece is provided with a second identification portion, and the second identification portion includes a plurality of second identification areas for identifying information of the buffer box; The first identification member and the second identification member are also provided with a first fool-proofing portion and a second fool-proofing portion, respectively. The first fool-proofing portion and the second fool-proofing portion cooperate with each other to match the buffer box and the reagent kit.

2. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 1, characterized in that: The first fool-proofing portion includes a plurality of first fool-proofing structures, and the second fool-proofing portion includes a plurality of second fool-proofing structures, and each of the first fool-proofing structures is matched with a corresponding second fool-proofing structure.

3. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 2, characterized in that: Each of the first fool-proofing structures is matched with a corresponding second fool-proofing structure, including the matching of the position and shape of each of the first fool-proofing structures with a corresponding second fool-proofing structure.

4. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 2 or 3, characterized in that: The first identification part and the second identification part are both arranged as a turntable structure, the rotation center axis of the first identification part is arranged along a direction parallel to the long side of the reagent box, and the rotation center axis of the second identification part is arranged along a direction perpendicular to the bottom surface of the buffer box.

5. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 4, characterized in that: The first identification area is arranged on the side of the turntable of the turntable structure of the first identification part, and the first fool-proofing part is vertically arranged on the bottom surface of the turntable.

6. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 5, characterized in that: The first fool-proofing part is also a rotating disk structure, and the first fool-proofing structure is arranged on the side of the rotating disk structure of the first fool-proofing part.

7. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 6, characterized in that: The first identification part and / or the first anti-foolproof part is a regular polygonal cylinder, and a first identification cavity is provided on the reagent kit. The shape of the first identification cavity is adapted to the shape of the first identification part and / or the first anti-foolproof part so that the first identification part cannot rotate after being installed in the first identification cavity.

8. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 4, characterized in that: The second identification area is arranged in a peripheral area of ​​the upper surface of the turntable of the turntable structure of the second identification portion, and the second fool-proofing portion is arranged on the lower surface of the turntable.

9. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 8, characterized in that: The second identification part is a regular polygonal cylinder, and a second identification cavity is provided on the buffer box. The shape of the second identification cavity is adapted to the shape of the second identification part so that the second identification part cannot rotate after being installed in the second identification cavity.

10. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 7, characterized in that: The first fool-proofing structure is a clamping groove arranged on each side surface of the regular polygonal column of the first fool-proofing part, and the second fool-proofing structure is a clamping column arranged on the second fool-proofing part and correspondingly adapted to the first fool-proofing structure.

11. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 7, characterized in that: The test kit includes a first identification box, the first identification cavity is arranged in the first identification box, the first identification cavity includes a first cavity for accommodating the first identification part and a second cavity for accommodating the first anti-foolproof part, a first rotating shaft is arranged at the bottom center of the first anti-foolproof part, and a rotating shaft groove is arranged on the side of the first identification box, and when the first identification part is installed in the first identification box, the first rotating shaft is clamped in the rotating shaft groove.

12. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 9, characterized in that: The buffer box includes a second identification box, the second identification cavity is a cavity opened downward in the second identification box, a second rotating shaft is arranged in the middle of the upper surface of the turntable of the second identification member, and a rotating shaft hole is arranged in the middle position of the top of the second identification box, and when the turntable of the second identification member is installed in the second identification box, the second rotating shaft is clamped into the rotating shaft hole.

13. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 11, characterized in that: The reagent kit is also provided with a reagent kit cover, and a first observation port is provided on the reagent kit cover corresponding to the first identification area.

14. The integrated device of a reagent kit and a buffer box for a gene sequencer according to claim 12, characterized in that: The second identification box is provided with an observation hole corresponding to the second identification area, and the buffer solution box is further provided with a buffer solution box cover, and the buffer solution box cover is provided with a second observation port corresponding to the observation hole.

15. A gene sequencer, characterized in that: It comprises an integrated device of a reagent kit and a buffer box for a gene sequencer as described in any one of claims 1 to 14, wherein the buffer box is arranged in a buffer box accommodating portion within the reagent kit body, the markings on the relative positions of the first identification member and the second identification member correspond to each other, and the first anti-foolproofing portion and the second anti-foolproofing portion are adapted to each other.

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