Stem cell incubator with detection function

By designing a stem cell incubator with detection function, using the combination of the liquid addition component and the cleaning component, the problem of contamination in the incubator cavity is solved, the survival rate and culture effect of stem cells are improved, and the status detection of the stem cell culture process is ensured.

CN119709401AInactive Publication Date: 2025-03-28FIRST AFFILIATED HOSPITAL OF XINJIANG MEDICAL UNIVERSITY
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Patent Information

Application Number
CN202411876653.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-19
Publication Date
2025-03-28
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

During the stem cell culture process, it is difficult for the prior art to avoid contamination in the incubator cavity when adding culture medium, resulting in stem cell death and affecting the culture effect.

Method used

A stem cell incubator with detection function was designed, using liquid addition and cleaning components. Through the cooperation of rotating connecting plates and magnetic blocks, the culture liquid is automatically added and contaminated. At the same time, the camera is cleaned through the cooperation of the servo motor and the synchronization belt.

Benefits of technology

It is achieved to avoid contamination in the incubator cavity when adding culture medium, improve the survival rate and culture effect of stem cells, ensure the cleanliness of the camera, and ensure the status detection of the stem cell culture process.

✦ Generated by Eureka AI based on patent content.

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Abstract

A stem cell culture box with a detection function belongs to the technical field of cell culture equipment and comprises a constant-temperature box, a liquid storage box is fixedly mounted at the position, close to the other side, of the top of the constant-temperature box, a liquid inlet pipe is inserted into the position, close to the other side, of the top of the liquid storage box, and a rotating shaft is rotatably connected to the middle of the bottom of an inner cavity of the constant-temperature box; a connecting disc A is fixedly mounted at the top end of the rotating shaft, a plurality of culture dishes are fixedly mounted at the top of the connecting disc A, and a germicidal lamp is fixedly mounted in the middle of the top of an inner cavity of the constant-temperature box. A culture solution can be automatically added into the inner cavity of the culture dish without manual addition of workers, the inner cavity of the incubator can be prevented from being polluted when the culture solution is added, and the survival rate of stem cell culture in the inner cavity of the culture dish is increased.
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Description

Technical Field

[0001] The invention relates to the technical field of cell culture equipment, in particular to a stem cell culture box with a detection function. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture technology is an important and commonly used technology in cell biology research methods. Through cell culture, we can not only obtain a large number of cells, but also study cell signal transduction, cell anabolism, cell growth and proliferation, etc.

[0003] When culturing stem cells in a cell culture incubator, when the culture fluid in the culture dish is low, the operator needs to add culture fluid to the culture dish. However, during the process of adding the culture fluid, the staff needs to open the door of the incubator to add it. This method can easily cause contamination inside the incubator and even cause the death of stem cells in the culture dish, affecting the effect of stem cell culture.

[0004] In view of the above problems, a stem cell culture box with detection function is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide a stem cell culture box with a detection function, and the device is used to work, thereby solving the above-mentioned problems.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a stem cell culture box with a detection function, comprising a constant temperature box, a liquid storage tank is fixedly installed on the top of the constant temperature box near the other side, a liquid inlet pipe is plugged into the top of the liquid storage tank near the other side, a rotating shaft is rotatably connected at the middle position of the bottom of the inner cavity of the constant temperature box, a connecting plate A is fixedly installed on the top of the rotating shaft, a plurality of culture dishes are fixedly installed on the top of the connecting plate A, a sterilization lamp is fixedly installed at the middle position of the top of the inner cavity of the constant temperature box, a liquid adding component is arranged near one side of the inner cavity of the constant temperature box, a cleaning component is arranged at the other side of the inner cavity of the constant temperature box, a connecting block is fixedly installed on the top of the inner cavity of the constant temperature box, and a connecting block is fixedly installed on the bottom end of the electric telescopic rod, and cameras are fixedly installed at the bottom of the connecting block near the front and rear sides;

[0007] The liquid adding assembly includes a hose plugged into the bottom of the inner cavity of the liquid storage tank and a solenoid valve sleeved on the outer periphery of the hose near the top end, a connecting pipe A is fixedly installed at the bottom end of the hose, a flexible tube is fixedly installed at the bottom end of the connecting pipe A, a connecting pipe B is fixedly installed at the bottom of the flexible tube, and a support plate is fixedly sleeved on the outer periphery of the connecting pipe A, and one side of the support plate is fixedly connected to the side wall of the inner cavity of the constant temperature box.

[0008] Furthermore, a hollow circular ring is fixedly installed near the top of the inner cavity of the connecting tube A, a rectangular block is fixedly installed near the middle position of the inner cavity of the connecting tube A, a magnetic block C is arranged on the top of the rectangular block, a rubber plug is fixedly installed at the middle position of the top of the magnetic block C, a fixed plate is fixedly installed near the top of the outer periphery of the connecting tube A, and a telescopic rod A is fixedly installed near the other side of the bottom of the fixed plate, the outer periphery of the connecting tube A is movably connected with the magnetic block B, and the outer periphery of the telescopic rod A is movably sleeved with a spring A, and the top and bottom ends of the spring A are fixedly connected to the fixed plate and the magnetic block B respectively, a driving block is fixedly installed on the top of the connecting disk A, a plurality of V-grooves are opened on the driving block, a T-shaped rod is movably penetrated through the inner cavity of adjacent V-grooves, and the front side of the T-shaped rod is fixedly connected to the magnetic block B; a plurality of magnetic columns A are fixedly installed on the top of the connecting disk A, and a magnetic block A is fixedly sleeved on the outer periphery of the connecting tube B.

[0009] Furthermore, a telescopic rod B is fixedly installed on one side of the rectangular block and the magnetic block C near both sides, and a spring B is movably sleeved on the outer periphery of the telescopic rod B, and both ends of the spring B are fixedly connected to the rectangular block and the magnetic block C respectively.

[0010] Furthermore, two limiting grooves are provided on the top of the magnetic block B near the other side, the inner cavities of the limiting grooves are movably passed through the limiting posts, and the tops of the limiting posts are fixedly connected to the fixing plate.

[0011] Furthermore, a controller is fixedly installed on one side of the thermostatic box, a sealed door is movably installed on the front side of the thermostatic box, and a transparent glass is fixedly installed on the front side of the sealed door.

[0012] Furthermore, a driving motor is fixedly installed at the bottom of the inner cavity of the constant temperature box, a driving disk is fixedly installed at the end of the power output shaft of the driving motor, a driving shaft is fixedly installed on the top of the driving disk near one side, a rotating disk is fixedly sleeved on the outer periphery of the rotating shaft near the top end, and the top end of the driving shaft moves through the inner cavity of the rotating disk.

[0013] Furthermore, the cleaning component includes a support block fixedly installed near the middle position on the rear side of the constant temperature box and a servo motor fixedly installed on the top of the support block. A threaded rod A is fixedly installed on the end of the servo motor power output shaft. The front end of the threaded rod A can move through the rear side of the inner cavity of the constant temperature box. A threaded rod B is rotatably connected to the other side of the inner cavity of the constant temperature box near the front side. The outer circumferences of the threaded rod A and the threaded rod B are threadedly connected with threaded sleeves, and a connecting disk B is fixedly installed on the bottom of the threaded sleeve, and a cleaning cloth is provided on the top of the connecting disk B.

[0014] Furthermore, the top of the connection plate B is fixedly mounted with a Velcro thorn-like surface, and the top of the Velcro thorn-like surface is provided with a Velcro hair-like surface, and the top of the Velcro hair-like surface is fixedly connected to the adjacent cleaning cloth respectively.

[0015] Furthermore, a driven bevel gear is fixedly sleeved on the outer periphery of the threaded rod B near the other end, a driving bevel gear is meshed with the rear side of the driven bevel gear, and a straight rod is fixedly installed at the rear center of the driving bevel gear, the rear end of the straight rod is rotatably connected to the rear side of the inner cavity of the constant temperature box, and synchronous wheels are fixedly sleeved on the outer periphery of the straight rod and the threaded rod A near the rear end, and a synchronous belt is commonly sleeved between the synchronous wheels.

[0016] Furthermore, a limiting block is fixedly installed on the top of the threaded sleeve, and a limiting rod movably penetrates the limiting block, and one end of the limiting rod is fixedly connected to the inner cavity side wall of the adjacent constant temperature box.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. When it is necessary to add culture fluid, the liquid adding component can be used in conjunction with the rotation of the connecting disk A. During the rotation of the connecting disk A, the culture fluid can be automatically added to the inner cavity of the culture dish without the need for manual addition by the staff. In addition, the inner cavity of the incubator can be prevented from being contaminated when the culture fluid is added, thereby improving the survival rate of stem cell culture in the inner cavity of the culture dish.

[0019] 2. During the rotation of the connecting disk A, the magnetic column A fixed on the top of the connecting disk A can be used in conjunction with the magnetic block A to bring the connecting tube B close to the culture dish, thereby preventing excess culture solution from leaking out of the inner cavity of the culture dish when the culture solution is added, thereby improving the cleanliness of the culture in the inner cavity of the constant temperature box.

[0020] 3. When the camera needs to be cleaned, the cleaning cloth can be moved and the camera can be cleaned with the help of the cleaning component, so as to avoid the camera being contaminated and affecting the subsequent status detection during the stem cell culture process. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0022] Figure 2 This is another perspective overall three-dimensional structural schematic diagram of the present invention;

[0023] Figure 3 It is a schematic diagram of a cutaway three-dimensional structure of the present invention;

[0024] Figure 4 It is a schematic diagram of the three-dimensional structure of the support block, the servo motor and the connecting plate A of the present invention;

[0025] Figure 5 It is a schematic diagram of the three-dimensional structure of the liquid adding assembly of the present invention;

[0026] Figure 6It is a schematic diagram of a partially cutaway three-dimensional structure of the connecting pipe A, the connecting pipe B and the supporting plate of the present invention;

[0027] Figure 7 It is a schematic diagram of the three-dimensional structure of the servo motor, cleaning cloth and synchronous belt of the present invention;

[0028] Figure 8 For the present invention Figure 6 Enlarged view of point A in the middle;

[0029] Fig. 9 For the present invention Figure 8 Enlarged view of point B in the middle;

[0030] Fig.10 For the present invention Figure 7 Enlarged view of point C in the middle.

[0031] In the figure: 1. Constant temperature box; 11. Controller; 12. Sealed door; 13. Transparent glass; 2. Liquid storage tank; 3. Rotating shaft; 31. Driving motor; 32. Driving disk; 33. Driving shaft; 34. Rotating disk; 4. Connecting disk A; 5. Culture dish; 6. Sterilizing lamp; 7. Adding liquid assembly; 71. Hose; 72. Connecting pipe A; 721. Fixed plate; 722. Telescopic rod A; 723. Magnetic block B; 7231. Limiting column; 724. Spring A; 725. Driving block; 726. T-bar; 727. Rectangular block; 728. Magnetic block C; 7281. Telescopic rod B; 7282. Spring B ;729, rubber plug;73, flexible tube;74, connecting tube B;741, magnetic block A;742, magnetic column A;75, support plate;8, cleaning assembly;81, servo motor;82, threaded rod A;821, synchronous belt;822, straight rod;823, active bevel gear;824, driven bevel gear;83, threaded rod B;84, connecting disk B;841, Velcro thorny surface;842, Velcro hairy surface;85, cleaning cloth;86, threaded sleeve;861, limit rod;862, limit block;87, support block;9, electric telescopic rod;10, connecting block;20, camera. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0034] The purpose of this embodiment is to provide a stem cell culture box with a detection function, including a constant temperature box 1, a liquid storage box 2 is fixedly installed at the top of the constant temperature box 1 near the other side, a liquid inlet pipe is inserted at the top of the liquid storage box 2 near the other side, a rotating shaft 3 is rotatably connected at the middle position of the bottom of the inner cavity of the constant temperature box 1, a connecting plate A4 is fixedly installed at the top of the rotating shaft 3, and a plurality of culture dishes 5 are fixedly installed on the top of the connecting plate A4, a sterilization lamp 6 is fixedly installed at the middle position of the top of the inner cavity of the constant temperature box 1, and the sterilization lamp 6 can sterilize the inner cavity of the constant temperature box 1, which is beneficial to the subsequent effect of stem cell culture, a liquid adding component 7 is provided near one side of the inner cavity of the constant temperature box 1, a cleaning component 8 is provided at the other side of the inner cavity of the constant temperature box 1, a connecting block 10 is fixedly installed at the top of the inner cavity of the constant temperature box 1, and a connecting block 10 is fixedly installed at the bottom end of the electric telescopic rod 9, and cameras 20 are fixedly installed at the bottom of the connecting block 10 near the front and rear sides;

[0035] The liquid adding assembly 7 includes a hose 71 inserted into the bottom of the inner cavity of the liquid storage tank 2 and a solenoid valve sleeved on the outer periphery of the hose 71 near the top end, a connecting pipe A72 is fixedly installed at the bottom end of the hose 71, a flexible tube 73 is fixedly installed at the bottom end of the connecting pipe A72, a connecting pipe B74 is fixedly installed at the bottom of the flexible tube 73, and a support plate 75 is fixedly sleeved on the outer periphery of the connecting pipe A72, and one side of the support plate 75 is fixedly connected to the inner cavity side wall of the constant temperature box 1.

[0036] Specifically, when it is necessary to add culture fluid, with the cooperation of the liquid adding component 7, the culture fluid can be automatically added to the inner cavity of the culture dish 5 under the rotation of the connecting disk A4 during the rotation of the connecting disk A4, without the need for manual addition by the staff, and can avoid contamination of the inner cavity of the constant temperature box 1 when adding culture fluid, thereby improving the survival rate of the stem cell culture in the inner cavity of the culture dish 5.

[0037] A hollow circular ring is fixedly installed near the top of the inner cavity of the connecting tube A72, a rectangular block 727 is fixedly installed near the middle position of the inner cavity of the connecting tube A72, a magnetic block C728 is arranged on the top of the rectangular block 727, a rubber plug 729 is fixedly installed at the middle position of the top of the magnetic block C728, a fixed plate 721 is fixedly sleeved near the top of the outer periphery of the connecting tube A72, and a telescopic rod A722 is fixedly installed near the other side of the bottom of the fixed plate 721, and a magnetic block B723 is movably connected to the outer periphery of the connecting tube A72, and the magnetic block B723 and the magnetic block C728 are opposite magnets, and the telescopic rod A722 is fixedly installed at the bottom of the fixed plate 721. A spring A724 is provided on the outer movable sleeve of the rod A722, and the top and bottom ends of the spring A724 are fixedly connected to the fixed plate 721 and the magnetic block B723 respectively; a driving block 725 is fixedly installed on the top of the connecting disk A4, and a plurality of V-shaped grooves are opened on the driving block 725. T-shaped rods 726 are movably penetrated through the inner cavities of adjacent V-shaped grooves, and the front side of the T-shaped rods 726 is fixedly connected to the magnetic block B723; a plurality of magnetic columns A742 are fixedly installed on the top of the connecting disk A4, and a magnetic block A741 is provided on the outer fixed sleeve of the connecting tube B74, and the magnetic columns A742 and the magnetic block A741 are arranged in opposite sexes.

[0038] Specifically, when the driving disk 32 rotates to a certain position, the rotating disk 34 can be rotated, and when the rotating disk 34 rotates, the support plate 75 can be rotated, thereby causing the magnetic block B723 and the magnetic block C728 to be misaligned. With the coordinated use of the telescopic rod B7281 and the spring B7282, the rubber plug 729 fixed on the magnetic block C728 can be inserted into the inner cavity of the hollow ring, thereby stopping the addition of culture fluid into the inner cavity of the culture dish 5.

[0039] A telescopic rod B7281 is fixedly installed near both sides of the opposite side of the rectangular block 727 and the magnetic block C728, and a spring B7282 is movably sleeved on the outer periphery of the telescopic rod B7281, and the two ends of the spring B7282 are fixedly connected to the rectangular block 727 and the magnetic block C728 respectively.

[0040] Specifically, when the T-shaped rod 726 is not located in the inner cavity of the V-shaped groove, the magnetic block B723 and the magnetic block C728 can be misaligned. Under the action of the spring B7282 and the telescopic rod B7281, the rubber plug 729 can be inserted into the inner cavity of the hollow ring, and the addition of culture medium is stopped.

[0041] Two limiting grooves are provided on the top of the magnetic block B723 near the other side. The inner cavities of the limiting grooves are movably passed through the limiting posts 7231 , and the tops of the limiting posts 7231 are fixedly connected to the fixing plate 721 .

[0042] Specifically, by setting the limiting groove and the limiting column 7231, the magnetic block B723 can be limited when moving.

[0043] A controller 11 is fixedly installed on one side of the thermostatic box 1 , a sealed door 12 is movably installed on the front side of the thermostatic box 1 , and a transparent glass 13 is fixedly installed on the front side of the sealed door 12 .

[0044] A driving motor 31 is fixedly installed at the bottom of the inner cavity of the constant temperature box 1, a driving disk 32 is fixedly installed at the end of the power output shaft of the driving motor 31, a driving shaft 33 is fixedly installed on the top of the driving disk 32 near one side, a rotating disk 34 is fixedly sleeved on the outer periphery of the rotating shaft 3 near the top, and the top of the driving shaft 33 moves through the inner cavity of the rotating disk 34.

[0045] Specifically, when the driving disk 32 rotates to a certain position, the rotating disk 34 can be rotated, and when the rotating disk 34 rotates, the supporting plate 75 can be rotated.

[0046] The cleaning component 8 includes a support block 87 fixedly installed at the rear side of the thermostatic box 1 near the middle position and a servo motor 81 fixedly installed on the top of the support block 87. A threaded rod A82 is fixedly installed on the end of the power output shaft of the servo motor 81. The front end of the threaded rod A82 can move through the rear side of the inner cavity of the thermostatic box 1. A threaded rod B83 is rotatably connected to the other side of the inner cavity of the thermostatic box 1 near the front side. The outer peripheries of the threaded rods A82 and B83 are threadedly connected with threaded sleeves 86. A connecting plate B84 is fixedly installed on the bottom of the threaded sleeves 86. A cleaning cloth 85 is provided on the top of the connecting plate B84. The cleaning cloth 85 is made of a water-absorbing material.

[0047] Specifically, when the camera 20 needs to be cleaned, the cleaning cloth 85 can be moved and the camera 20 can be cleaned with the cooperation of the cleaning component 8, so as to prevent the camera 20 from being contaminated and affecting the subsequent status detection during the stem cell culture process.

[0048] The top of the connection plate B84 is fixedly mounted with a Velcro thorn-like surface 841 , and the top of the Velcro thorn-like surface 841 is provided with a Velcro hairy surface 842 , and the top of the Velcro hairy surface 842 is fixedly connected to the adjacent cleaning cloth 85 .

[0049] Specifically, by providing the Velcro fur-like surface 842 and the Velcro fur-like surface 842 , the cleaning cloth 85 can be replaced, which is beneficial for subsequent cleaning.

[0050] A driven bevel gear 824 is fixedly sleeved on the outer circumference of the threaded rod B83 near the other end, and a driving bevel gear 823 is meshed with the rear side of the driven bevel gear 824, and a straight rod 822 is fixedly installed at the rear center of the driving bevel gear 823, and the rear end of the straight rod 822 is rotatably connected to the rear side of the inner cavity of the constant temperature box 1. Synchronous wheels are fixedly sleeved on the outer circumference of the straight rod 822 and the threaded rod A82 near the rear end, and a synchronous belt 821 is commonly sleeved between the synchronous wheels.

[0051] A limiting block 862 is fixedly installed on the top of the threaded sleeve 86 , and a limiting rod 861 movably penetrates the limiting block 862 , and one end of the limiting rod 861 is fixedly connected to the inner cavity side wall of the adjacent constant temperature box 1 .

[0052] Specifically, the threaded sleeve 86 can be stably moved by providing the limiting block 862 and the limiting rod 861 .

[0053] In summary, the working principle of the present invention is as follows: when it is necessary to add culture fluid to the inner cavity of the culture dish 5, the solenoid valve can be started by the controller 11, and when the connecting disk A4 rotates, the culture dish 5 fixed on the connecting disk A4 can be located at the bottom of the connecting tube B74, and the magnetic column A742 and the magnetic block A741 can be used together to make the connecting tube B74 close to the culture dish 5, thereby preventing the excess culture fluid from leaking out of the inner cavity of the culture dish 5 when the culture fluid is added, thereby improving the cleanliness of the culture in the inner cavity of the incubator 1. When the T-shaped rod 726 is located in the inner cavity of the adjacent V-shaped groove, the telescopic rod A722 and the spring A724 can be used together to enable the magnetic block B723 to move downward, and then the magnetic block B723 can be moved downward under the limit of the two limit posts 7231. Through the use of the magnetic block C728, the rubber plug 729 can be kept away from the inner cavity of the hollow ring, so that the liquid in the inner cavity of the liquid storage tank 2 can be added to the inner cavity of the culture dish 5. When the driving disk 32 rotates to a certain position, the rotating disk 3 4 rotates, and when the rotating disk 34 rotates, the support plate 75 can rotate, so that the magnetic block B723 and the magnetic block C728 can be misaligned. With the coordinated use of the telescopic rod B7281 and the spring B7282, the rubber plug 729 fixed on the magnetic block C728 can be inserted into the inner cavity of the hollow ring, so as to stop adding culture fluid to the inner cavity of the culture dish 5. When it is necessary to detect the stem cells in the inner cavity of the culture dish 5, the servo motor 81 is first started by the controller 11, so that the rubber plug 729 fixed on the servo motor 81 can be inserted into the inner cavity of the hollow ring. The threaded rod A82 at the end of the power output shaft rotates, and the threaded rod B83 can be rotated under the cooperation of the synchronous belt 821, the straight rod 822, the active bevel gear 823 and the driven bevel gear 824. The threaded sleeve 86 threadedly connected to the outer periphery of the threaded rod B83 can move the connecting disk B84 fixed at the bottom of the threaded sleeve 86. The camera 20 can be cleaned by the cleaning cloth 85 located on the top of the connecting disk B84, which can prevent water vapor from blocking the camera 20 and affecting the observation of the state during the stem cell culture process.

[0054] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A stem cell culture box with a detection function, comprising a constant temperature box (1), characterized in that: A liquid storage tank (2) is fixedly installed on the top of the thermostatic box (1) near the other side, a liquid inlet pipe is inserted on the top of the liquid storage tank (2) near the other side, a rotating shaft (3) is rotatably connected at the middle position of the bottom of the inner cavity of the thermostatic box (1), a connecting plate A (4) is fixedly installed on the top of the rotating shaft (3), a plurality of culture dishes (5) are fixedly installed on the top of the connecting plate A (4), a sterilizing lamp (6) is fixedly installed at the middle position of the top of the inner cavity of the thermostatic box (1), a liquid adding component (7) is arranged near one side of the inner cavity of the thermostatic box (1), a cleaning component (8) is arranged on the other side of the inner cavity of the thermostatic box (1), a connecting block (10) is fixedly installed on the top of the inner cavity of the thermostatic box (1), and an electric A connecting block (10) is fixedly mounted on the bottom end of the telescopic rod (9), and cameras (20) are fixedly mounted on the bottom of the connecting block (10) near the front and rear sides; the liquid adding assembly (7) comprises a hose (71) plugged into the bottom of the inner cavity of the liquid storage tank (2) and a solenoid valve sleeved on the outer periphery of the hose (71) near the top end; a connecting pipe A (72) is fixedly mounted on the bottom end of the hose (71), a flexible pipe (73) is fixedly mounted on the bottom end of the connecting pipe A (72), a connecting pipe B (74) is fixedly mounted on the bottom of the flexible pipe (73), and a supporting plate (75) is fixedly sleeved on the outer periphery of the connecting pipe A (72), and one side of the supporting plate (75) is fixedly connected to the inner cavity side wall of the thermostatic box (1).

2. The stem cell culture box with detection function according to claim 1, characterized in that: A hollow circular ring is fixedly installed near the top of the inner cavity of the connecting tube A (72); a rectangular block (727) is fixedly installed near the middle of the inner cavity of the connecting tube A (72); a magnetic block C (728) is arranged on the top of the rectangular block (727); a rubber plug (729) is fixedly installed at the middle of the top of the magnetic block C (728); a fixed plate (721) is fixedly sleeved near the top of the outer periphery of the connecting tube A (72); a telescopic rod A (722) is fixedly installed near the other side of the bottom of the fixed plate (721); a magnetic block B (723) is movably connected to the outer periphery of the connecting tube A (72); and the telescopic rod A spring A (724) is provided on the outer peripheral movable sleeve of A (722), and the top and bottom ends of the spring A (724) are respectively fixedly connected to the fixed plate (721) and the magnetic block B (723); a driving block (725) is fixedly installed on the top of the connecting disk A (4), and a plurality of V-shaped grooves are provided on the driving block (725), and a T-shaped rod (726) is movably penetrated through the inner cavity of adjacent V-shaped grooves, and the front side of the T-shaped rod (726) is fixedly connected to the magnetic block B (723); a plurality of magnetic columns A (742) are fixedly installed on the top of the connecting disk A (4), and a magnetic block A (741) is provided on the outer peripheral fixed sleeve of the connecting tube B (74).

3. The stem cell culture box with detection function according to claim 2, characterized in that: A telescopic rod B (7281) is fixedly installed near both sides of the rectangular block (727) and the magnetic block C (728), and a spring B (7282) is movably sleeved on the outer periphery of the telescopic rod B (7281), and the two ends of the spring B (7282) are fixedly connected to the rectangular block (727) and the magnetic block C (728) respectively.

4. The stem cell culture box with detection function according to claim 2, characterized in that: Two limiting grooves are provided at the top of the magnetic block B (723) near the other side, the inner cavities of the limiting grooves are movably penetrated through the limiting posts (7231), and the tops of the limiting posts (7231) are fixedly connected to the fixing plate (721).

5. The stem cell culture box with detection function according to claim 1, characterized in that: A controller (11) is fixedly mounted on one side of the thermostatic box (1), a sealed door (12) is movably mounted on the front side of the thermostatic box (1), and a transparent glass (13) is fixedly mounted on the front side of the sealed door (12).

6. The stem cell culture box with detection function according to claim 1, characterized in that: A driving motor (31) is fixedly mounted at the bottom of the inner cavity of the thermostatic box (1); a driving disk (32) is fixedly mounted on the end of the power output shaft of the driving motor (31); a driving shaft (33) is fixedly mounted on the top of the driving disk (32) near one side; a rotating disk (34) is fixedly sleeved on the outer periphery of the rotating shaft (3) near the top end; and the top end of the driving shaft (33) movably passes through the inner cavity of the rotating disk (34).

7. The stem cell culture box with detection function according to claim 1, characterized in that: The cleaning assembly (8) comprises a support block (87) fixedly mounted at a position near the middle of the rear side of the thermostatic box (1) and a servo motor (81) fixedly mounted on the top of the support block (87); a threaded rod A (82) is fixedly mounted on the end of the power output shaft of the servo motor (81); the front end of the threaded rod A (82) movably penetrates the rear side of the inner cavity of the thermostatic box (1); a threaded rod B (83) is rotatably connected to the other side of the inner cavity of the thermostatic box (1) near the front side; threaded sleeves (86) are threadedly connected to the outer circumferences of the threaded rod A (82) and the threaded rod B (83); a connecting disk B (84) is fixedly mounted on the bottom of the threaded sleeve (86); and a cleaning cloth (85) is arranged on the top of the connecting disk B (84).

8. The stem cell culture box with detection function according to claim 7, characterized in that: The top of each connection plate B (84) is fixedly mounted with a Velcro thorn-like surface (841), and the top of each Velcro thorn-like surface (841) is provided with a Velcro fur-like surface (842), and the top of each Velcro fur-like surface (842) is fixedly connected to an adjacent cleaning cloth (85).

9. The stem cell culture box with detection function according to claim 7, characterized in that: A driven bevel gear (824) is fixedly sleeved on the outer periphery of the threaded rod B (83) near the other end, a driving bevel gear (823) is meshed with the rear side of the driven bevel gear (824), and a straight rod (822) is fixedly installed at the center of the rear side of the driving bevel gear (823), the rear end of the straight rod (822) is rotatably connected to the rear side of the inner cavity of the thermostatic box (1), and synchronous wheels are fixedly sleeved on the outer peripheries of the straight rod (822) and the threaded rod A (82) near the rear end, and a synchronous belt (821) is sleeved between the synchronous wheels.

10. The stem cell culture box with detection function according to claim 7, characterized in that: A limiting block (862) is fixedly installed on the top of the threaded sleeve (86), and a limiting rod (861) is movably passed through the limiting block (862), and one end of the limiting rod (861) is fixedly connected to the inner cavity side wall of the adjacent constant temperature box (1).