Construction method and system of Kelvin Cell tissue engineering auricle
By constructing tissue-engineered auricle with Kelvin Cell structure, the problem of the intra-art auricle scaffolding in the body is difficult to resist stress and maintain shape accurately in the body, achieving efficient energy absorption and good elasticity of the auricle, and promoting tissue regeneration.
Patent Information
- Application Number
- CN202411902905.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-23
- Publication Date
- 2025-03-28
AI Technical Summary
Existing tissue-engineered auricle scaffolds are difficult to simulate the microstructure of the natural auricle, making it difficult to resist stress and maintain shape accurately in the body.
By obtaining the auricle digital model, a tissue-engineered auricle with a Kelvin Cell structure is constructed using 3D printing technology. The Kelvin Cell structure is a tetrahedral lattice structure with excellent energy absorption and good elasticity.
The precise shape maintenance and stress resistance of tissue engineered auricle in the body is achieved, which promotes tissue regeneration and significantly improves the mechanical properties of the auricle.
Smart Images
Figure CN119720311A_ABST
Abstract
Description
Background Art
[0002] The integration of structure and function of tissue engineering auricle scaffold is of great significance for bionic natural auricle. Natural auricle cartilage mainly bears compressive stress and can undergo large deformation and return to its original state, which is closely related to its special structure: at the microscopic level, elastic fibers wrap each chondrocyte to form a three-dimensional honeycomb lattice, which has extremely strong energy absorption and good elasticity, and the Kelvin Cell structure has better energy absorption than the honeycomb structure.
[0003] Kelvin Cell structure: It is a tetradecahedral lattice structure composed of 6 quadrilaterals and 8 hexagons, which has excellent energy absorption and is close to isotropy. It can simulate the cuttlefish skeleton and cancellous bone in nature, which have excellent mechanical properties due to their lattice structure, in an idealized model. The structure is mainly based on compression characteristics. Although it sacrifices the strength of the structure to a certain extent, it can deform uniformly under compression stress to avoid catastrophic damage. Summary of the invention
[0004] The present application provides a method and system for constructing a Kelvin Cell tissue-engineered auricle, which is used to simulate the microstructure of a natural auricle to obtain a tissue-engineered auricle that can resist stress and accurately maintain its shape in vivo.
[0005] In a first aspect, a method for constructing a Kelvin Cell tissue-engineered auricle is provided, comprising the following steps:
[0006] Obtain a digital model of the pinna;
[0007] Modeling is performed using the auricle digital model to obtain an original auricle model;
[0008] Processing the original auricle model to obtain a Kelvin Cell structure auricle model;
[0009] The Kelvin Cell structure auricle model is 3D printed to obtain a bionic tissue engineering auricle with a Kelvin Cell structure.
[0010] In the above technical scheme, a digital model of the auricle is obtained; the digital model of the auricle is used for modeling to obtain an original auricle model; the original auricle model is processed to obtain an auricle model with a Kelvin Cell structure; the Kelvin Cell structure auricle model is 3D printed to obtain a bionic tissue-engineered auricle with a Kelvin Cell structure; and a tissue-engineered auricle that can resist stress and accurately maintain its shape in vivo is obtained by biomimetic simulation of the microstructure of the natural auricle.
[0011] In a specific implementation scheme, the step of obtaining the digital model of the auricle specifically includes:
[0012] The real human auricle is 3D scanned and preprocessed to obtain a 3D model of the human auricle.
[0013] The temporal bone part of the three-dimensional model of the human auricle is removed, and only the auricle part is retained to obtain the auricle digital model.
[0014] In a specific implementation scheme, the step of processing the original auricle model to obtain the auricle model with Kelvin Cell structure specifically includes:
[0015] Reserve a rim at the edge of the original auricle model;
[0016] The array model is embedded into the original auricle model by utilizing the edge wrapping to obtain the auricle model of the Kelvin Cell structure.
[0017] In a specific implementation scheme, the step of creating the array model includes:
[0018] Design unit lattice,
[0019] The unit cell is array-amplified to obtain the array model.
[0020] In a specific implementation scheme, the step of 3D printing the Kelvin Cell structure auricle model to obtain a bionic tissue engineering auricle with a Kelvin Cell structure specifically includes:
[0021] The auricle model with Kelvin Cell structure is sliced and 3D printed using biological ink mixed with seed cells to obtain the bionic tissue engineering auricle with Kelvin Cell structure.
[0022] In a specific implementation scheme, the three-dimensional model of the human auricle is constructed by using one or more of CT scanning, laser scanning, and MRI scanning.
[0023] In a specific implementation scheme, the three-dimensional model of the human auricle is cut using one or more of Mimics Medical, CAD, Creo, Potato OSP, and Zbrush.
[0024] In a specific embodiment, the seed cells are one or more of chondrocytes, adipose stem cells, bone marrow stem cells, and mesenchymal stem cells.
[0025] In a specific embodiment, the bio-ink is one or more of methacrylate decellularized matrix, methacrylate gelatin, methacrylate hyaluronic acid, methacrylate sodium alginate, methacrylate silk protein, methacrylate chitosan, methacrylate chondroitin sulfate, and methacrylate elastin.
[0026] In a second aspect, a Kelvin Cell tissue engineering auricle construction system is provided, comprising:
[0027] An auricle digital model module, used for obtaining an auricle digital model;
[0028] An original auricle model module is used to perform modeling using the auricle digital model to obtain an original auricle model;
[0029] A Kelvin Cell structure auricle module, used to process the original auricle model to obtain a KelvinCell structure auricle model;
[0030] The printing module is used to perform 3D printing on the Kelvin Cell structure auricle model to obtain a bionic tissue engineering auricle with a Kelvin Cell structure.
[0031] In the above technical scheme, a digital model of the auricle is obtained; the digital model of the auricle is used for modeling to obtain an original auricle model; the original auricle model is processed to obtain an auricle model with a Kelvin Cell structure; the Kelvin Cell structure auricle model is 3D printed to obtain a bionic tissue-engineered auricle with a Kelvin Cell structure; and a tissue-engineered auricle that can resist stress and accurately maintain its shape in vivo is obtained by biomimetic simulation of the microstructure of the natural auricle. BRIEF DESCRIPTION OF THE DRAWINGS
[0032] Figure 1 A flowchart of the method for constructing a Kelvin Cell tissue engineering auricle provided in an embodiment of the present application;
[0033] Figure 2 This is a structural block diagram of the system for constructing the Kelvin Cell tissue engineering auricle provided in the embodiments of the present application. DETAILED DESCRIPTION
[0034] The present application is further described in detail below through the accompanying drawings and embodiments. Through these descriptions, the characteristics and advantages of the present application will become clearer and more specific.
[0035] The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration." Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments. Although various aspects of the embodiments are shown in the drawings, the drawings are not necessarily drawn to scale unless otherwise noted.
[0036] In addition, the technical features involved in different embodiments of the present application described below can be combined with each other as long as they do not conflict with each other.
[0037] To facilitate understanding of the construction method and system of the Kelvin Cell tissue engineering auricle provided in the embodiment of the present application, its application scenario is first explained. The construction method and system of the Kelvin Cell tissue engineering auricle provided in the embodiment of the present application are used to biomimetic the anisotropy of the natural auricle, and obtain a tissue engineering auricle that can resist stress and accurately maintain its shape in vivo. The integration of the structure and function of the tissue engineering auricle scaffold is of great significance for biomimetic natural auricle. The natural auricle cartilage mainly bears compressive stress, can undergo large deformation and restore its original state, which is closely related to its special structure: microscopically, elastic fibers wrap each chondrocyte to form a three-dimensional honeycomb lattice, which has extremely strong energy absorption and good elasticity, and the Kelvin Cell structure has better energy absorption than the honeycomb structure. Kelvin Cell structure: It is a tetradecahedral lattice structure composed of 6 quadrilaterals and 8 hexagons with excellent energy absorption, close to isotropy. It can simulate the cuttlefish skeleton, cancellous bone, etc. in nature that have excellent mechanical properties due to the lattice structure in an idealized model. The structure is mainly based on compression characteristics. Although the strength of the structure is sacrificed to a certain extent, it can be uniformly deformed under compressive stress to avoid catastrophic damage. To this end, the embodiment of the present application provides a method and system for constructing a Kelvin Cell tissue engineering auricle to mimic the microstructure of a natural auricle and obtain a tissue engineering auricle that can resist stress and accurately maintain its shape in vivo. The following is a detailed description of the embodiment in conjunction with specific drawings.
[0038] refer to Figure 1 and Figure 2 , Figure 1 A flowchart of the method for constructing a Kelvin Cell tissue engineering auricle provided in an embodiment of the present application; Figure 2 This is a structural block diagram of the system for constructing the Kelvin Cell tissue engineering auricle provided in the embodiments of the present application.
[0039] exist Figure 1 In the present application, an embodiment provides a method for constructing a Kelvin Cell tissue engineering auricle, comprising the following steps:
[0040] Obtain a digital model of the pinna;
[0041] Modeling is performed using the auricle digital model to obtain an original auricle model;
[0042] Processing the original auricle model to obtain a Kelvin Cell structure auricle model;
[0043] The Kelvin Cell structure auricle model is 3D printed to obtain a bionic tissue engineering auricle with a Kelvin Cell structure.
[0044] In the above technical scheme, a digital model of the auricle is obtained; the digital model of the auricle is used for modeling to obtain an original auricle model; the original auricle model is processed to obtain an auricle model with a Kelvin Cell structure; the Kelvin Cell structure auricle model is 3D printed to obtain a bionic tissue-engineered auricle with a Kelvin Cell structure; and a tissue-engineered auricle that can resist stress and accurately maintain its shape in vivo is obtained by biomimetic simulation of the microstructure of the natural auricle.
[0045] In a specific implementation scheme, the step of obtaining the digital model of the auricle specifically includes:
[0046] The real human auricle is 3D scanned and preprocessed to obtain a 3D model of the human auricle.
[0047] The temporal bone part of the three-dimensional model of the human auricle is removed, and only the auricle part is retained to obtain the auricle digital model.
[0048] In a specific implementation scheme, the step of processing the original auricle model to obtain the auricle model with Kelvin Cell structure specifically includes:
[0049] Reserve a rim at the edge of the original auricle model;
[0050] The array model is embedded into the original auricle model by utilizing the edge wrapping to obtain the auricle model of the Kelvin Cell structure.
[0051] In a specific implementation scheme, the step of creating the array model includes:
[0052] Design unit lattice,
[0053] The unit cell is array-amplified to obtain the array model.
[0054] In a specific implementation scheme, the step of 3D printing the Kelvin Cell structure auricle model to obtain a bionic tissue engineering auricle with a Kelvin Cell structure specifically includes:
[0055] The auricle model with Kelvin Cell structure is sliced and 3D printed using biological ink mixed with seed cells to obtain the bionic tissue engineering auricle with Kelvin Cell structure.
[0056] In a specific implementation scheme, the three-dimensional model of the human auricle is constructed by using one or more of CT scanning, laser scanning, and MRI scanning.
[0057] In a specific implementation scheme, the three-dimensional model of the human auricle is cut using one or more of Mimics Medical, CAD, Creo, Potato OSP, and Zbrush.
[0058] In a specific embodiment, the seed cells are one or more of chondrocytes, adipose stem cells, bone marrow stem cells, and mesenchymal stem cells.
[0059] In a specific embodiment, the bio-ink is one or more of methacrylate decellularized matrix, methacrylate gelatin, methacrylate hyaluronic acid, methacrylate sodium alginate, methacrylate silk protein, methacrylate chitosan, methacrylate chondroitin sulfate, and methacrylate elastin.
[0060] Specifically, the method for constructing the Kelvin Cell tissue-engineered auricle optimizes the tissue-engineered auricle scaffold structure and constructs the Kelvin Cell auricle structure by 3D printing, in order to obtain a tissue-engineered auricle that can resist stress and accurately maintain its shape in vivo. The method includes the following steps:
[0061] (1) The real human auricle morphology was 3D scanned and processed by a CAD computer-aided design system, and then a mirrored 3D model of the human auricle was obtained using Boolean operations. The temporal bone part of the 3D model of the human auricle was removed using Mimics Medical 21.0 software, leaving only the auricle part, and the digital model of the auricle in STL format was exported.
[0062] (2) The digital model of the auricle was imported into SolidWorks 2023 software for modeling, and a 0.5-1 mm thick edging was reserved on the edge of the original auricle model.
[0063] (3) In Creo 5.0 software, a unit lattice with equal long and wide diameters (0.5-5 mm) and a regular hexagonal side length of (0.5-7) mm was designed and its array was expanded. Then, the array model was embedded into the auricle model through design parameters, modular construction, and Boolean operations such as merging or adding and subtracting auricle and lattice modules. This is the Kelvin Cell auricle model.
[0064] (4) The nested model was divided into corresponding layers with a height of 50 to 200 μm, imported into the PotatoOSP-Client V1.2.6 software, sliced with each layer of 50 to 200 μm, and uploaded to the 3D printer for printing.
[0065] (5) Adding bio-ink mixed with seed cells to the 3D printer, adjusting the light intensity, exposure time, base layer exposure time, and base layer number to an appropriate level, and then printing, a bionic tissue engineering auricle with a Kelvin Cell structure can be obtained.
[0066] Preferably, the three-dimensional digital model of the human auricle can be constructed using one or more of CT, laser scanning, MRI, etc.;
[0067] Preferably, the 3D model cutting software can use one or more of Mimics Medical, CAD, Creo, PotatoOSP, and Zbrush;
[0068] Preferably, the 3D bioprinter can be selected from one or more of projection light-curing printer, extrusion printing combined with suspension printing, and dynamic interface printing;
[0069] Preferably, the seed cells may be one or more of chondrocytes, adipose stem cells, bone marrow stem cells, mesenchymal stem cells and the like.
[0070] Preferably, the bio-ink can be a combination of one or more of methacrylate decellularized matrix, methacrylate gelatin, methacrylate hyaluronic acid, methacrylate sodium alginate, methacrylate silk protein, methacrylate chitosan, methacrylate chondroitin sulfate, and methacrylate elastin.
[0071] In this embodiment, the microstructure of the natural auricle can be biomimetic, and its beneficial effects include:
[0072] (1) Excellent energy absorption:
[0073] The Kelvin Cell structure has better energy absorption than the honeycomb structure. In the finite element analysis experiment, when subjected to force, a relatively uniform stress distribution can be seen on its lattice structure, indicating that it can buffer stress well.
[0074] (2) Highly porous channel structure is conducive to tissue growth:
[0075] In in vitro and in vivo implantation experiments, Kelvin Cell structure constructs can promote the massive growth of cells and tissues, and promote the growth of abundant blood vessels in the body.
[0076] (3) Enhanced stability of regenerated tissue: A thicker and denser capsule is formed on the surface of the Kelvin Cell structure with a larger pore size. The capsule tissue is tightly anchored and inseparable from the regenerated cartilage tissue, indicating that this structure is more conducive to promoting the mechanical interlocking between the surrounding tissue and the implant. This phenomenon will enhance the mechanical stability of the structure.
[0077] Precise distribution of cells and materials: The integration of 3D bioprinting technology can achieve precise spatial distribution of cells and materials, which not only solves the problem of morphological control, but also ensures the directional distribution of cells and materials.
[0078] In the above technical solution, natural ear cartilage has excellent elasticity due to the three-dimensional honeycomb elastic fiber network surrounding the chondrocytes, and can return to its original shape after a large deformation. The present invention relates to a bionic 3D printed tissue engineering auricle with a Kelvin Cell structure, which biomimics the microstructure of the natural auricle. The Kelvin Cell structure refers to: a tetradecahedral lattice structure composed of six quadrilaterals and eight hexagons with excellent energy absorption, close to isotropy, and has extremely strong energy absorption and good elasticity. The present application obtains a Kelvin Cell structure tissue engineering auricle model by embedding a specific array lattice structure into a digital three-dimensional model of a real human ear, and it can be accurately prepared by 3D printing. This model can significantly promote tissue regeneration both in vivo and in vitro, and can resist stress and accurately maintain the shape of the auricle.
[0079] exist Figure 2 In the present application, an embodiment provides a system for constructing a Kelvin Cell tissue engineering auricle, comprising:
[0080] An auricle digital model module, used for obtaining an auricle digital model;
[0081] An original auricle model module is used to perform modeling using the auricle digital model to obtain an original auricle model;
[0082] A Kelvin Cell structure auricle module, used to process the original auricle model to obtain a KelvinCell structure auricle model;
[0083] The printing module is used to perform 3D printing on the Kelvin Cell structure auricle model to obtain a bionic tissue engineering auricle with a Kelvin Cell structure.
[0084] In the above technical scheme, a digital model of the auricle is obtained; the digital model of the auricle is used for modeling to obtain an original auricle model; the original auricle model is processed to obtain an auricle model with a Kelvin Cell structure; the Kelvin Cell structure auricle model is 3D printed to obtain a bionic tissue-engineered auricle with a Kelvin Cell structure; and a tissue-engineered auricle that can resist stress and accurately maintain its shape in vivo is obtained by biomimetic simulation of the microstructure of the natural auricle.
[0085] Those skilled in the art will appreciate that the present application may be implemented as a system, method or computer program product.
[0086] Therefore, the present disclosure may be specifically implemented in the following forms, namely: it may be completely hardware, it may be completely software (including firmware, resident software, microcode, etc.), or it may be a combination of hardware and software, generally referred to herein as a "circuit", "module" or "system". In addition, in some embodiments, the present application may also be implemented in the form of a computer program product in one or more computer-readable media, and the computer-readable medium may contain computer-readable program code.
[0087] Any combination of one or more computer-readable media can be used. Computer-readable media can be computer-readable signal media or computer-readable storage media. Computer-readable storage media can be, for example, but not limited to, electrical, magnetic, optical, electromagnetic, infrared, or semiconductor systems, devices or devices, or any combination of the above. More specific examples (non-exhaustive list) of computer-readable storage media include: electrical connections with one or more wires, portable computer disks, hard disks, random access memories (RAM), read-only memories (ROM), erasable programmable read-only memories (EPROM or flash memory), optical fibers, portable compact disk read-only memories (CD-ROM), optical storage devices, magnetic storage devices, or any suitable combination of the above. In this document, computer-readable storage media can be any tangible medium containing or storing a program, which can be used by an instruction execution system, device or device or used in combination with it.
[0088] Although the embodiments of the present application have been shown and described above, it is understood that the above embodiments are exemplary and cannot be understood as limiting the present application. A person of ordinary skill in the art can change, modify, replace and modify the above embodiments within the scope of the present application. On this basis, a variety of replacements and improvements can be made to the present application, all of which fall within the scope of protection of the present application.
Claims
1. A method for constructing a Kelvin Cell tissue engineering auricle, characterized in that It is able to absorb energy and resist stress extremely well, and can maintain a precise shape in the body for a long time, including the following steps: Obtain a digital model of the pinna; Modeling is performed using the auricle digital model to obtain an original auricle model; Processing the original auricle model to obtain a Kelvin Cell structure auricle model; The Kelvin Cell structure auricle model is 3D printed to obtain a bionic tissue engineering auricle with a Kelvin Cell structure.
2. The method for constructing auricle of Kelvin Cell tissue engineering according to claim 1, characterized in that: The steps for obtaining a digital model of the auricle include: The real human auricle is 3D scanned and preprocessed to obtain a 3D model of the human auricle. The temporal bone part of the three-dimensional model of the human auricle is removed, and only the auricle part is retained to obtain the auricle digital model.
3. The method for constructing auricle of Kelvin Cell tissue engineering according to claim 2, characterized in that: The step of processing the original auricle model to obtain the auricle model with Kelvin Cell structure specifically includes: Reserve a rim around the edge of the original auricle model; The array model is embedded into the original auricle model by utilizing the edge wrapping to obtain the auricle model of the Kelvin Cell structure.
4. The method for constructing auricle of Kelvin Cell tissue engineering according to claim 3, characterized in that: The steps of creating the array model include: Design unit lattice, The unit cell is array-amplified to obtain the array model.
5. The method for constructing auricle of Kelvin Cell tissue engineering according to claim 4, characterized in that: The step of 3D printing the Kelvin Cell structure auricle model to obtain a bionic tissue engineering auricle with a Kelvin Cell structure specifically includes: The auricle model with Kelvin Cell structure is sliced and 3D printed using biological ink mixed with seed cells to obtain the bionic tissue engineering auricle with Kelvin Cell structure.
6. The method for constructing auricle of Kelvin Cell tissue engineering according to claim 5, characterized in that: The three-dimensional model of the human auricle is constructed by using one or more of CT scanning, laser scanning, and MRI scanning.
7. The method for constructing auricle of Kelvin Cell tissue engineering according to claim 6, characterized in that: The three-dimensional model of the human auricle is cut using one or more of Mimics Medical, CAD, Creo, PotatoOSP, and Zbrush.
8. The method for constructing auricle of Kelvin Cell tissue engineering according to claim 7, characterized in that: The seed cells are one or more of chondrocytes, adipose stem cells, bone marrow stem cells, and mesenchymal stem cells.
9. The method for constructing auricle of Kelvin Cell tissue engineering according to claim 8, characterized in that: The biological ink is one or more of methacrylate decellularized matrix, methacrylate gelatin, methacrylate hyaluronic acid, methacrylate sodium alginate, methacrylate silk protein, methacrylate chitosan, methacrylate chondroitin sulfate, and methacrylate elastin.
10. A system for constructing auricle using Kelvin Cell tissue engineering, characterized in that: include: An auricle digital model module, used for obtaining an auricle digital model; An original auricle model module is used to perform modeling using the auricle digital model to obtain an original auricle model; A Kelvin Cell structure auricle module, used for processing the original auricle model to obtain a Kelvin Cell structure auricle model; The printing module is used to perform 3D printing on the auricle model with Kelvin Cell structure to obtain a bionic tissue engineering auricle with Kelvin Cell structure.