Traditional Chinese medicine composition for treating chronic enteritis

Abstract

The invention provides a traditional Chinese medicine composition for treating chronic enteritis, and relates to the technical field of traditional Chinese medicine pharmacy. The invention provides a traditional Chinese medicine composition for treating chronic enteritis. The traditional Chinese medicine composition is prepared from felwort, radix sophorae flavescentis, cortex phellodendri, rhizoma sparganii, oroxylum indicum, areca-nut, radix paeoniae alba, fructus rosae laevigatae, yellow halloysite, codonopsis pilosula, mangnolia officinalis, semen raphani and peach blossom. The traditional Chinese medicine composition has the effects of clearing heat and drying dampness, invigorating spleen and replenishing qi, promoting the circulation of qi and removing food retention, relieving diarrhea with astringents and promoting blood circulation to arrest pain. The traditional Chinese medicine composition disclosed by the invention aims at regulating the overall function of a body, enhances transportation and transformation of spleen and stomach, and improves qi and blood circulation and water metabolism of intestinal tracts, so that the purpose of treating chronic enteritis is achieved. The traditional Chinese medicine composition disclosed by the invention can be used for remarkably relieving weight loss, excrement condition and hemafecia condition of a DSS-induced chronic enteritis mouse model, remarkably improving colon shortening condition and colon tissue pathological change of the DSS-induced chronic enteritis mouse model, recovering damaged tissue structures, reducing the number and infiltration range of inflammatory cells, and improving the survival rate of the chronic enteritis mouse model. Good anti-inflammatory and anti-inflammatory effects are achieved. The traditional Chinese medicine composition disclosed by the invention has a wide application prospect in preparation of medicines for treating chronic enteritis.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine pharmaceutical preparations, and in particular to a traditional Chinese medicine composition for treating chronic enteritis. Background Art

[0002] Chronic enteritis is a common disease in gastroenterology. The main clinical symptoms of patients are long-term, chronic, recurrent diarrhea, abdominal pain, loss of appetite, abnormal stools and increased frequency. With the changes and diversification of modern dietary structure, the incidence of chronic enteritis is increasing. The disease often requires colonoscopy, which can detect pathological manifestations such as mucosal congestion or erosion, and has a high disease diagnosis rate. The cause of the disease is complex and it is not easy to cure. At the same time, the recurrence rate is high, and it may even progress to intestinal tumors. Therefore, effective control of symptoms is of great significance to improving the quality of life of patients.

[0003] At present, clinical treatment often uses immune preparations and amino acid salicylic acid preparations to help patients control the development of the disease, but the recurrence rate is high after drug withdrawal, which increases the difficulty of treatment. With the widespread promotion of Chinese medicine in the clinic in recent years, it has been applied to the clinical treatment of various diseases and has achieved more significant therapeutic effects. According to Chinese medicine theory, chronic enteritis belongs to the category of reading and writing, diarrhea, with large and small intestines, spleen and stomach as the main lesion sites, and is closely related to organs such as kidney and liver. When the human body is emotionally disturbed, mentally tense, and stagnant liver qi, it will easily cause spleen deficiency and dampness, metabolic disorders, etc., and cause chronic enteritis lesions. The application of Chinese medicine in the clinical treatment of digestive diseases is conducive to further improving the overall efficacy of patients, and has fewer adverse reactions and higher safety. The present invention aims to provide a Chinese medicine composition with high efficacy in the treatment of chronic enteritis and significantly improves the clinical symptoms of patients. Summary of the invention

[0004] 1. Technical issues to be solved

[0005] In view of the deficiencies of the prior art, the present invention provides a traditional Chinese medicine composition for treating chronic enteritis.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] The invention provides a traditional Chinese medicine composition for treating chronic enteritis. The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 5-20 parts of gentian, 5-20 parts of sophora flavescens, 5-20 parts of phellodendron, 1-15 parts of trillium, 1-15 parts of oroxylum butterfly, 1-15 parts of areca catechu, 5-20 parts of white peony, 5-20 parts of rosa laevigata, 5-15 parts of phellodendron chinense, 5-20 parts of codonopsis pilosula, 5-20 parts of magnolia bark, 1-15 parts of radish seeds and 1-10 parts of peach blossom.

[0009] Furthermore, the Chinese medicine composition is composed of the following raw materials in parts by weight: 10-20 parts of gentian, 5-15 parts of sophora flavescens, 10-20 parts of phellodendron, 5-15 parts of trillium, 5-15 parts of oroxylum, 1-10 parts of areca catechu, 5-15 parts of white peony, 10-20 parts of rose laevigata, 5-10 parts of yellow ochre, 10-20 parts of codonopsis pilosula, 5-15 parts of magnolia bark, 5-15 parts of radish seeds, and 1-6 parts of peach blossom.

[0010] Furthermore, the Chinese medicine composition is composed of the following raw materials in parts by weight: 16 parts of gentian, 10 parts of sophora flavescens, 14 parts of phellodendron, 10 parts of trillium, 10 parts of oroxylum, 8 parts of areca catechu, 12 parts of white peony root, 16 parts of rose laevigata, 8 parts of phellodendron chinense, 15 parts of codonopsis pilosula, 12 parts of magnolia bark, 10 parts of radish seeds, and 4 parts of peach blossom.

[0011] The present invention also provides the use of the above-mentioned traditional Chinese medicine composition in the preparation of drugs for treating chronic enteritis. The traditional Chinese medicine composition can be prepared into any oral dosage form acceptable to the human body according to conventional medical preparation methods, and the dosage form includes oral liquid, decoction, granules, pills, freeze-dried powder, powder, etc.

[0012] Pharmacological effects of each medicinal material in the Chinese medicine composition of the present invention:

[0013] Gentiana scabra: bitter in taste, cold in nature, enters the liver and gallbladder meridians; has the effects of clearing away heat and dampness, and purging liver and gallbladder fire; used for damp-heat jaundice, vaginal swelling and itching, leucorrhea, eczema itching, liver-fire red eyes, tinnitus and deafness, flank pain and bitter mouth, strong middle, and convulsions.

[0014] Sophora flavescens: bitter, cold. It has the functions of clearing away heat and dampness, killing insects, and promoting diuresis. It is used for heat dysentery, blood in stool, jaundice and urine retention, leucorrhea, vaginal swelling and itching, eczema, wet sores, skin itching, scabies and leprosy, and external treatment of trichomonas vaginitis.

[0015] Huangbai: bitter and cold in nature; enters the kidney and bladder meridians; clears away heat and dampness, purges fire and eliminates steaming, detoxifies and treats sores; used for damp-heat diarrhea, jaundice and dark urine, leucorrhea and vulvar itching, hot and painful stranguria, beriberi and paralysis, bone steaming and fatigue fever, night sweats, spermatorrhea, sores and swelling, eczema and wet sores.

[0016] Tripterygium wilfordii: bitter, pungent and neutral in nature. "Supplement to Materia Medica" says it is sweet, neutral and warm. "Return to the Channels" says it enters the liver and spleen. Functions and indications: it can break blood and promote qi circulation, eliminate stagnation and relieve pain, treat accumulation of phlegm and masses, stagnation of qi and blood, pain in the heart and abdomen, pain under the ribs, amenorrhea, abdominal pain caused by blood stasis after childbirth, injuries from falls, and hard sores. "Kaibao Materia Medica" says it is used to treat phlegm and masses caused by old age.

[0017] Oroxylum butterfly: bitter, sweet, cool. Enters the lung, liver, and stomach meridians. Clears the lungs and relieves sore throat, soothes the liver and harmonizes the stomach. Treats lung heat cough, throat numbness, hoarseness, and liver and stomach qi pain. "Supplement to Compendium of Materia Medica" says: "It treats heart qi pain, liver qi pain, and damp heat in the lower body. Xiang Qiuzi also said that if the carbuncle does not heal, apply this paste on it."

[0018] Betel nut: bitter, pungent, warm, enters the stomach and large intestine meridians; kills insects, eliminates accumulation, promotes qi, promotes diuresis, and stops malaria; used for tapeworms, ascariasis, ginger worm disease, abdominal pain caused by worm accumulation, diarrhea, tenesmus, edema, beriberi, and malaria

[0019] White Peony Root: bitter, sour, slightly cold, enters the Liver and Spleen Meridians; nourishes blood and softens the liver, relieves pain, astringes yin and reduces sweat; mainly treats pain in the chest, abdomen, ribs, diarrhea, abdominal pain, spontaneous sweating, night sweats, fever due to yin deficiency, irregular menstruation, metrorrhagia, and leukorrhea.

[0020] Rosa Laevigata: tastes sour, sweet, astringent, and is neutral in nature; it enters the kidney, bladder, and large intestine meridians; it has the effects of consolidating semen and reducing urine, consolidating metrorrhagia and stopping leukorrhea, and astringing the intestines to stop diarrhea; it is used for symptoms such as spermatorrhea, enuresis and frequent urination, metrorrhagia and leukorrhea, and chronic diarrhea and dysentery; Rosa Laevigata mainly contains effective chemical ingredients such as polysaccharides, triterpenes, tannins, and flavonoids; modern pharmacological studies have shown that Rosa Laevigata has antioxidant, immune regulatory, blood sugar and lipid lowering, antibacterial and anti-inflammatory, and anti-tumor effects, and also has good antipyretic, antidiarrheal, analgesic and hemostatic effects.

[0021] Huangshizhi: A medicinal mineral of yellow clay with hydromica or kaolinite as the main component; bitter, flat, and enters the spleen and large intestine meridians; has the effects of strengthening the spleen and astringing the intestines, stopping bleeding and healing sores; mainly treats diarrhea, pus and blood; carbuncle and malignant sores; sores that do not heal for a long time

[0022] Codonopsis: sweet, neutral, non-toxic, enters the spleen and lung meridians; has the effects of tonifying the middle, replenishing qi, and promoting body fluid, and is used for spleen and stomach weakness, qi and blood deficiency, fatigue, lack of appetite, thirst, chronic diarrhea, prolapse of the anus, etc. Codonopsis has the effects of enhancing immunity, dilating blood vessels, lowering blood pressure, improving microcirculation, enhancing hematopoietic function, etc., and has the effect of enhancing the body's resistance.

[0023] Magnolia officinalis: bitter and pungent in taste; warm in nature; enters the spleen, stomach, lung and large intestine meridians; dries dampness and eliminates phlegm, and relieves gas and distension; used for dampness stagnation and damage to the middle part of the body, abdominal distension, vomiting and diarrhea, food accumulation and qi stagnation, abdominal distension and constipation, and cough with phlegm and fluid.

[0024] Radish seeds: sweet in taste, neutral in nature, enter the lung and stomach meridians; used to relieve asthma and relieve gas, digest food and resolve phlegm. Treats cough, phlegm, asthma, food accumulation, qi stagnation, chest tightness, abdominal distension, and heaviness after diarrhea.

[0025] Peach blossom: bitter, flat, has diuretic, blood-activating and laxative effects; treats edema, athlete's foot, phlegm, stagnation, difficulty in defecation and urination, and amenorrhea.

[0026] (III) Beneficial effects

[0027] The invention provides a Chinese medicine composition for treating chronic enteritis, which is composed of gentian, sophora flavescens, phellodendron, triloba, oroxylum, areca nut, white peony root, golden rosa laevigata, yellow ochre, codonopsis, magnolia bark, radish seed and peach blossom. In the prescription, gentian, sophora flavescens and phellodendron are used as monarch drugs. Gentian clears heat and dries dampness, and purges liver and gallbladder fire. Sophora flavescens clears heat and dries dampness, kills insects and is diuretic. Phellodendron clears heat and dries dampness, purges fire and detoxifies, and removes fever and steam. The three are used together to play the effect of clearing heat and drying dampness, and mainly attack the symptoms of damp-heat in the intestine. With yellow ochre, triloba and peach blossom as ministerial drugs, yellow ochre astringes the intestine and stops diarrhea, and is assisted by the monarch drug to relieve diarrhea symptoms; triloba breaks blood and promotes qi circulation, eliminates accumulation and relieves pain, and peach blossom is diuretic and laxative, promotes blood circulation and removes blood stasis. On the basis of the monarch drug clearing heat and drying dampness, it dissipates intestinal qi and blood stasis, and assists the monarch drug to improve intestinal inflammation. In addition, codonopsis, magnolia bark, oroxylum, areca nut, white peony root, rosa laevigata fruit, and radish seed are used as adjuvants. Codonopsis pilosula invigorates the spleen and lungs, nourishes blood and produces body fluid, magnolia bark dries dampness and eliminates phlegm, relieves qi and eliminates fullness, oroxylum soothes the liver and harmonizes the stomach, areca nut promotes qi and eliminates accumulation, white peony root softens the liver and relieves pain, relieves urgency and stops pain, rosa laevigata fruit astringes the intestines and stops diarrhea, and radish seed helps digestion and eliminates bloating. The main and secondary drugs are used together to play the effects of clearing away heat and drying dampness, invigorating the spleen and replenishing qi, promoting qi and eliminating accumulation, astringing the intestines and stopping diarrhea, and promoting blood circulation and stopping pain. The Chinese medicine composition of the present invention aims to regulate the overall function of the body, enhance the spleen and stomach transportation, improve the intestinal qi and blood circulation, and water metabolism, so as to achieve the purpose of treating chronic enteritis.

[0028] The Chinese medicine composition of the present invention can significantly alleviate the weight loss, feces and blood in stool of the chronic enteritis mouse model induced by DSS, has a significant therapeutic effect on the chronic enteritis mouse model, can significantly improve the shortening of the colon and the pathological changes of the colon tissue in the chronic enteritis mouse model induced by DSS, restore the damaged tissue structure, and reduce the number and infiltration range of inflammatory cells. The Chinese medicine composition of the present invention reduces the progression of inflammation in the chronic enteritis mouse model by down-regulating the pro-inflammatory factors (IL-1β and TNF-α) in the colon tissue of the chronic enteritis mouse model induced by DSS, up-regulating the expression of anti-inflammatory factors (IL-10), and has a good anti-inflammatory and anti-inflammatory effect. The Chinese medicine composition of the present invention has a wide range of application prospects in the preparation of drugs for the treatment of chronic enteritis. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 DAI scores of mice in each group; compared with the model group, **P<0.01, ***P<0.001.

[0030] Figure 2 The colon length measurement results (A) and colon photos (B) of mice in each group; compared with the model group, **P<0.01.

[0031] Figure 3 The colon tissue pathology scores of mice in each group; compared with the model group, **P<0.01, ***P<0.001.

[0032] Figure 4The relative expression levels of inflammatory factors IL-1β (A), TNF-α (B), and IL-10 (C) in the colon tissue of mice in each group; compared with the model group, *P<0.05, **P<0.01, ***P<0.001. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] Example 1

[0035] A traditional Chinese medicine composition for treating chronic enteritis, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 5 parts of gentian, 5 parts of sophora flavescens, 5 parts of phellodendron, 3 parts of trillium, 3 parts of oroxylum butterfly, 3 parts of areca catechu, 5 parts of white peony, 5 parts of rosa laevigata, 5 parts of phellodendron chinense, 5 parts of codonopsis pilosula, 5 parts of magnolia bark, 3 parts of radish seeds, and 3 parts of peach blossom.

[0036] The above-mentioned Chinese medicine composition is used to prepare a drug for treating chronic enteritis. The Chinese medicine composition can be prepared into any oral dosage form acceptable to the human body according to conventional medical preparation methods, including oral liquid, decoction, granules, pills, freeze-dried powder, powder, etc.

[0037] Example 2

[0038] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of gentian, 20 parts of sophora flavescens, 20 parts of phellodendron, 15 parts of trillium, 15 parts of oroxylum, 15 parts of areca catechu, 20 parts of white peony, 20 parts of rose laevigata, 15 parts of phellodendron chinense, 20 parts of codonopsis pilosula, 20 parts of magnolia bark, 15 parts of radish seeds, and 10 parts of peach blossom.

[0039] Example 3

[0040] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 8 parts of gentian, 8 parts of sophora flavescens, 6 parts of phellodendron, 3 parts of trillium, 3 parts of oroxylum, 5 parts of areca catechu, 8 parts of white peony, 8 parts of cherry laevigata, 6 parts of phellodendron chinense, 8 parts of codonopsis pilosula, 8 parts of magnolia bark, 4 parts of radish seeds, and 3 parts of peach blossom.

[0041] Example 4

[0042] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 18 parts of gentian, 15 parts of sophora flavescens, 16 parts of phellodendron, 12 parts of trillium, 10 parts of oroxylum, 12 parts of areca catechu, 16 parts of white peony, 18 parts of rose laevigata, 12 parts of yellow ochre, 18 parts of codonopsis pilosula, 16 parts of magnolia bark, 10 parts of radish seeds, and 8 parts of peach blossom.

[0043] Example 5

[0044] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 10 parts of gentian, 5 parts of sophora flavescens, 10 parts of phellodendron, 5 parts of trillium, 5 parts of oroxylum, 3 parts of areca catechu, 5 parts of white peony, 10 parts of rose laevigata, 5 parts of yellow ochre, 10 parts of codonopsis pilosula, 5 parts of magnolia bark, 5 parts of radish seeds, and 2 parts of peach blossom.

[0045] Example 6

[0046] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of gentian, 15 parts of sophora flavescens, 20 parts of phellodendron, 15 parts of trillium, 15 parts of oroxylum, 10 parts of areca catechu, 15 parts of white peony root, 20 parts of rose laevigata, 10 parts of yellow ochre, 20 parts of codonopsis pilosula, 15 parts of magnolia bark, 15 parts of radish seeds, and 6 parts of peach blossom.

[0047] Example 7

[0048] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 14 parts of gentian, 12 parts of sophora flavescens, 12 parts of phellodendron, 8 parts of trillium, 8 parts of oroxylum, 4 parts of areca catechu, 10 parts of white peony, 12 parts of rose laevigata, 6 parts of phellodendron chinense, 12 parts of codonopsis pilosula, 10 parts of magnolia bark, 8 parts of radish seeds, and 3 parts of peach blossom.

[0049] Example 8

[0050] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 16 parts of gentian, 10 parts of sophora flavescens, 14 parts of phellodendron, 10 parts of trillium, 10 parts of oroxylum, 8 parts of areca catechu, 12 parts of white peony root, 16 parts of rose laevigata, 8 parts of yellow ochre, 15 parts of codonopsis pilosula, 12 parts of magnolia bark, 10 parts of radish seeds, and 4 parts of peach blossom.

[0051] Test Example 1

[0052] Anti-inflammatory effect of the Chinese medicine composition of the present invention on intestinal mucosal microvascular endothelial cells (RIMVECs)

[0053] 1 Method

[0054] 1.1 Preparation of LPS solution

[0055] The LPS powder was diluted with high-glucose DMEM solution, mixed thoroughly, and prepared into a 1 mg / mL solution, which was frozen in a -20°C refrigerator; the 1 mg / mL LPS solution was taken out of the refrigerator, thawed, shaken and mixed, diluted with high-glucose DMEM, and prepared into a 40 μg / mL LPS solution, and then FBS and insulin injection were added to make the concentrations 3% and 0.27%, respectively.

[0056] 1.2 Preparation of test drug solution

[0057] Drug 1: 16 parts of Gentiana, 10 parts of Sophora flavescens, 14 parts of Phellodendron amurense, 10 parts of Tripterygium wilfordii, 10 parts of Oroxylum indica, 8 parts of Areca catechu, 12 parts of White Peony Root, 16 parts of Rosa laevigata Fruit, 8 parts of Phellodendron chinense, 15 parts of Codonopsis pilosula, 12 parts of Magnolia officinalis, 10 parts of Radish Seed, 4 parts of Peach Blossom

[0058] Drug 2: 16 parts of Gentiana scabra, 14 parts of Phellodendron chinense, 10 parts of Tripterygium wilfordii, 10 parts of Oroxylum indica, 8 parts of Areca catechu, 8 parts of Phellodendron chinense, 15 parts of Codonopsis pilosula, 12 parts of Magnolia officinalis, 10 parts of Radish seeds, 4 parts of Peach Blossom

[0059] Drug 3: 10 parts of Sophora flavescens, 10 parts of Tripterygium wilfordii, 10 parts of Oroxylum indica, 8 parts of Areca catechu, 12 parts of White Peony Root, 16 parts of Rosa laevigata, 8 parts of Phellodendron chinense, 15 parts of Codonopsis pilosula, 12 parts of Magnolia officinalis

[0060] Drug 4: 16 parts of Gentiana, 10 parts of Sophora flavescens, 14 parts of Phellodendron amurense, 12 parts of White Peony Root, 16 parts of Rosa Laevigata Fruit, 8 parts of Phellodendron chinense, 15 parts of Codonopsis pilosula, 12 parts of Magnolia officinalis, 10 parts of Radish Seed, 4 parts of Peach Blossom

[0061] Drug 5: 16 parts of Gentiana, 10 parts of Sophora flavescens, 14 parts of Phellodendron amurense, 10 parts of Tripterygium wilfordii, 10 parts of Oroxylum indica, 8 parts of Areca catechu, 12 parts of White Peony Root, 16 parts of Rosa laevigata Fruit, 10 parts of Radish Seed, 4 parts of Peach Blossom

[0062] Weigh each raw material according to the above weight proportions, add 4 times water and boil twice, filter, combine the decoctions, concentrate to a Chinese medicine stock solution 1-5 with a drug content of 1g / mL (equivalent to 1g of crude drug per milliliter), sterilize, refrigerate and store for later use; when used, first use DMSO solvent to prepare a test mother solution with a concentration of 25mg / mL, then dilute it with high-glucose DMEM to a solution with a drug concentration of 2.5mg / mL, mix well and add 3% FBS to obtain a test drug 1-5 solution.

[0063] 1.3 Protective effect on LPS-induced RIMVECs cells

[0064] The cultured RIMVECs cells were incubated at 10 4The cells were plated in a 96-well plate at a density of 100 μL / well and cultured in a cell culture incubator for 12 h. The supernatant in each well was discarded and the corresponding PLS solution and test drug 1-5 solution were added respectively. The blank control group was added with maintenance culture medium. Each group was set up with 6 replicates and cultured in a cell culture incubator for 6 h. The supernatant was collected for later use. 100 μL of DMEM solution with a concentration of 10% CCK8 was added to the cells. After standing at 37°C in the dark for 1.5 h, the OD value was measured at 450 nm on a microplate reader to calculate the RIMVECs cell activity.

[0065] Cell viability (%) = (OD 试验 -OD 空白 ) / (OD 对照 -OD 空白 )×100%

[0066] 1.4 Detection of inflammatory factors in LPS-induced RIMVECs

[0067] ELISA was used to detect the levels of TNF-α, IL-6, and IL-1β in the cell supernatant

[0068] 1.5 Data Analysis

[0069] Statistical analysis was performed using Prism 8.0, and one-way analysis of variance was used for comparison among groups. The results are expressed as mean ± standard deviation The data were analyzed using t-test. P < 0.05 was considered statistically significant.

[0070] 2 Results

[0071] 2.1 Protective effect of the Chinese medicine composition of the present invention on LPS-induced RIMVECs cells

[0072] The results are shown in Table 1. Compared with the blank control group, LPS can cause significant damage to RIMVECs cells and significantly reduce cell activity. Compared with the positive control group, the cell activity of test groups 1-5 showed different degrees of increase, and the difference was significant (P<0.05). Among them, the cell activity of test group 1 can reach 96.26%, which is significantly higher than that of test groups 2-5 (P<0.05).

[0073] Table 1 Cell activity of LPS-induced RIMVECs cells under the action of different drugs

[0074]

[0075]

[0076] Note: The same lowercase letters on the shoulders indicate P>0.05; different lowercase letters on the shoulders indicate P<0.05.

[0077] 2.2 Effect of the Chinese medicine composition of the present invention on the secretion of inflammatory factors by RIMVECs cells induced by LPS

[0078] The results are shown in Table 2. Compared with the blank control group, LPS stimulation of RIMVECs cells significantly increased the expression of inflammatory factors (TNF-α, IL-6, IL-1β) in RIMVECs cells, with significant differences (P<0.05); compared with the positive control group, under the action of test drugs 1-5, the expression of inflammatory factors (TNF-α, IL-6, IL-1β) in RIMVECs cells of test groups 1-5 decreased to varying degrees, with significant differences (P<0.05); among them, the expression of inflammatory factors (TNF-α, IL-6, IL-1β) in RIMVECs cells of test group 1 was significantly lower than that of test groups 2-5, with significant differences (P<0.05).

[0079] Table 2 Secretion levels of inflammatory factors in LPS-induced RIMVECs cells under the action of different drugs

[0080] Grouping TNF-α(pg / mL) IL-6 (pg / mL) IL-1β (pg / mL) Blank control group <![CDATA[30.02±5.03 a ]]> <![CDATA[24.16±2.74 a ]]> <![CDATA[32.39±4.09 a ]]> Positive control group <![CDATA[123.36±7.99 b ]]> <![CDATA[69.15±3.49 b ]]> <![CDATA[113.09±8.44 b ]]> Experimental Group 1 <![CDATA[42.11±3.97 c ]]> <![CDATA[31.16±1.84 c ]]> <![CDATA[46.97±5.66 c ]]> Experimental Group 2 <![CDATA[74.19±6.87 d ]]> <![CDATA[49.10±3.12 d ]]> <![CDATA[78.14±6.07 d ]]> Experimental Group 3 <![CDATA[83.16±5.47 d ]]> <![CDATA[46.31±2.71 de ]]> <![CDATA[71.26±5.22 d ]]> Experimental Group 4 <![CDATA[66.19±8.01 e ]]> <![CDATA[40.26±3.11 ef ]]> <![CDATA[62.18±3.74 e ]]> Experimental Group 5 <![CDATA[58.92±4.77 e ]]> <![CDATA[38.48±5.06 f ]]> <![CDATA[57.19±4.89 e ]]>

[0081] Note: The same lowercase letters on the shoulders indicate P>0.05; different lowercase letters on the shoulders indicate P<0.05.

[0082] 3 Conclusion

[0083] The above results show that high concentrations of LPS can cause RIMVECs cell damage and the development of inflammation. The test drugs 1-5 provided in this experiment all showed varying degrees of protective and anti-inflammatory effects on cell damage induced by high concentrations of LPS, but the various components of the Chinese medicine composition of the present invention (test drug 1) synergistically enhanced and cooperated with each other to achieve the best protection and anti-inflammatory effects on LPS-induced cell damage.

[0084] Test Example 2

[0085] Effect of the Chinese medicine composition of the present invention on the DSS-induced chronic enteritis mouse model

[0086] 1 Materials and methods

[0087] 1.1 Experimental animals

[0088] Fifteen 6-8 week old SPF male BALB / c mice were selected and housed in an SPF environment, following the circadian rhythm and maintaining a suitable temperature and humidity, with indoor temperature of 22-26°C and relative humidity of 50%-60%.

[0089] 1.2 Preparation of the Chinese medicine stock solution of the present invention

[0090] 16 parts of Gentiana scabra, 10 parts of Sophora flavescens, 14 parts of Phellodendron amurense, 10 parts of Tripterygium wilfordii, 10 parts of Oroxylum indica, 8 parts of Areca catechu, 12 parts of White Peony Root, 16 parts of Rosa laevigata Fruit, 8 parts of Phellodendron chinense, 15 parts of Codonopsis pilosula, 12 parts of Magnolia officinalis, 10 parts of Radish Seed, 4 parts of Peach Blossom

[0091] 1.2 Animal grouping, modeling and drug treatment

[0092] Before the experiment, the mice were adaptively fed for 1 week, and then divided into 3 groups with 5 mice in each group using a random number table method. The healthy control group was fed normally and drank sterile mineral water freely; the remaining 2 groups of mice drank sterile mineral water containing 10g / LDS for 7 days, and then drank sterile distilled water for 7d, with 2 weeks as a cycle (3 cycles in total) to establish a mouse chronic enteritis model; the experimental group was gavaged with the Chinese medicine stock solution of the present invention (10g / kg·mouse) every day from the second day after the mice began to drink the DSS solution until the end of the experiment.

[0093] 1.3 Observation indicators

[0094] 1.3.1 Mouse weight, feces and blood in stool

[0095] The weight, feces and blood in the stool of the mice were recorded, and the DAI score was calculated according to the literature to calculate the enteritis disease activity index (DAI). The specific scoring criteria are:

[0096] Mouse weight: no weight loss is 0 points; weight loss of 1% to 5% is 1 point; weight loss of 5% to 10% is 2 points; weight loss of 10% to 20% is 3 points; weight loss of more than 20% is 4 points.

[0097] Stool: 0 points for normal stool; 1 point for soft stool; 2 points for wet and soft stool; 3 points for semi-loose stool; 4 points for loose stool.

[0098] Blood in stool: No blood in stool is 0 points; positive is 2 points; pure blood in stool is 4 points.

[0099] DAI = (weight score + stool characteristics score + stool blood score) / 3, DAI is distributed from 0 (healthy) to 4 (the most severe colitis symptoms).

[0100] 1.3.2 Colon length measurement

[0101] After the treatment, the mice were killed by cervical dislocation, the intestinal tissue from the end of the cecum to the anus was separated, and the length from the anorectal end to the junction of the colon and cecum was measured.

[0102] 1.3.3 Colon histological pathological scoring

[0103] The inflamed colon tissue of mice was rinsed twice with precooled 0.9% sodium chloride injection, and 1 cm of rectal tissue close to the anus was soaked in paraformaldehyde for 24 hours, dehydrated, paraffin-embedded, sliced ​​and HE stained. A 0-3 point system was used to describe the severity of inflammation, and the three parameters were added together, with the highest score being 9. The scoring table is shown in Table 3.

[0104] Table 3 Colonic histopathological scores

[0105]

[0106] 1.3.4 Detection of inflammatory factors in colon tissue

[0107] Total RNA was extracted from mouse colonic inflammation tissue using Trizol reagent. Quantitative PCR was performed strictly according to the protocol provided by the manufacturer. The primer sequence list is shown in Table 4.

[0108] Table 4 Primer sequences

[0109]

[0110] 1.4 Data Analysis

[0111] Statistical analysis was performed using Prism 8.0, and one-way analysis of variance was used for comparison among groups. The results are expressed as mean ± standard deviation The data were analyzed using t-test. P < 0.05 was considered statistically significant.

[0112] 2 Results

[0113] 2.1 Effect of the Chinese medicine composition of the present invention on the DAI score of the chronic enteritis mouse model

[0114] Compared with the healthy control group, mice began to eat less, lose weight, and have loose stools on the third day after freely drinking DSS-containing drinking water. As time went on, the mice in the model group became listless and lazy, and their food intake decreased significantly, with watery and even bloody stools. The weight, stool, and bloody stools of the mice in the experimental group were also affected. Compared with the healthy control group, the DAI scores of the mice in the model group were significantly increased (P<0.001); compared with the model group, the DAI scores of the mice in the experimental group were significantly decreased (P<0.01). Figure 1 shown.

[0115] 2.2 Effect of the Chinese medicine composition of the present invention on colon length and histopathological score of chronic enteritis mouse model

[0116] The colon length of mice in each group was measured and it was found that the colon length of mice in the model group was significantly shorter than that of the healthy control group, with a significant difference (P<0.01); compared with the model group, the colon length of mice in the experimental group was significantly longer, with a significant difference (P<0.01). Figure 2 shown.

[0117] The results of colon tissue pathology of mice in each group showed that ( Figure 3 ), compared with the healthy control group, the colon pathology score of the mice in the model group was significantly increased, the difference was extremely significant (P<0.001); compared with the model group, the colon pathology score of the mice in the experimental group was significantly decreased, the difference was significant (P<0.01).

[0118] 2.3 Effect of the Chinese medicine composition of the present invention on inflammatory factors in colon tissue of chronic enteritis mouse model

[0119] Compared with the healthy control group, the relative expression of IL-1β and TNF-α inflammatory factors in the colon tissue of the chronic enteritis mouse model in the model group was significantly increased (P<0.001), and the relative expression of IL-10 inflammatory factor was significantly decreased (P<0.01); compared with the model group, the relative expression of IL-1β and TNF-α inflammatory factors in the colon tissue of the chronic enteritis mouse model in the experimental group was significantly decreased (P<0.001), and the relative expression of IL-10 inflammatory factor was significantly decreased (P<0.05). The above results show. Results as Figure 4 shown.

[0120] 3 Conclusion

[0121] The above results show that intragastric administration of the Chinese medicine composition of the present invention can significantly alleviate the weight loss, feces and blood in the DSS-induced chronic enteritis mouse model, has a significant therapeutic effect on chronic enteritis mice, and can significantly improve the shortening of the colon and pathological changes in colon tissue in the DSS-induced chronic enteritis mouse model, restore damaged tissue structure, and reduce the number and infiltration range of inflammatory cells. The Chinese medicine composition of the present invention has good anti-inflammatory and anti-inflammatory effects by down-regulating the expression of pro-inflammatory factors (IL-1β and TNF-α) in the colon tissue of the DSS-induced chronic enteritis mouse model and up-regulating the expression of anti-inflammatory factors (IL-10), thereby alleviating the progression of inflammation in the chronic enteritis mouse model.

[0122] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine composition for treating chronic enteritis, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 5-20 parts of gentian, 5-20 parts of sophora flavescens, 5-20 parts of phellodendron, 1-15 parts of trillium, 1-15 parts of oroxylum butterfly, 1-15 parts of areca catechu, 5-20 parts of white peony, 5-20 parts of rose laevigata, 5-15 parts of yellow ochre, 5-20 parts of codonopsis pilosula, 5-20 parts of magnolia bark, 1-15 parts of radish seeds, and 1-10 parts of peach blossom.

2. A Chinese medicine composition for treating chronic enteritis according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 10-20 parts of gentian, 5-15 parts of sophora flavescens, 10-20 parts of phellodendron, 5-15 parts of trifoliate lancea, 5-15 parts of oroxylum butterflybone, 1-10 parts of areca catechu, 5-15 parts of white peony root, 10-20 parts of rosa laevigata, 5-10 parts of yellow ochre, 10-20 parts of codonopsis pilosula, 5-15 parts of magnolia bark, 5-15 parts of radish seeds, and 1-6 parts of peach blossom.

3. A Chinese medicine composition for treating chronic enteritis according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 16 parts of gentian, 10 parts of sophora flavescens, 14 parts of phellodendron, 10 parts of trillium, 10 parts of oroxylum, 8 parts of areca catechu, 12 parts of white peony root, 16 parts of rosa laevigata, 8 parts of phellodendron chinense, 15 parts of codonopsis pilosula, 12 parts of magnolia bark, 10 parts of radish seeds, and 4 parts of peach blossom.

4. Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a drug for treating chronic enteritis, characterized in that: The Chinese medicine composition can be prepared into any oral dosage form acceptable to the human body according to conventional medical preparation methods, and the dosage form includes oral liquid, decoction, granules, pills, freeze-dried powder, powder, etc.

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