Application of raspberry aqueous extract in preparation of medicine for preventing or treating liver injury

Abstract

The invention discloses an application of a raspberry aqueous extract in preparation of a medicine for preventing or treating liver injury, and a preparation method of the raspberry aqueous extract comprises the following steps: washing raspberry with clear water, repeatedly soaking the raspberry with deionized water, merging the soak solutions, decocting, filtering, taking the decoction, decocting again, and concentrating the decoction to obtain the raspberry aqueous extract. The raspberry aqueous extract preparation has the characteristics of remarkable curative effect, safety, reliability, small toxic and side effects and the like when being used for treating the metabolism-related fatty liver diseases; from the whole, the metabolism-related fatty liver diseases are intervened in a multi-component and multi-target mode, the levels of ALT and AST in plasma of a model mouse and cholesterol and triglyceride in liver tissue are remarkably reduced, liver fat change and liver lipid accumulation are relieved, and good clinical application prospects are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine, and particularly relates to an application of a raspberry water extract in preparing a medicine for preventing or treating liver damage. Background Art

[0002] Metabolic dysfunction-associated steatotic liver disease (MASLD) develops from simple fatty liver and is the most common chronic liver disease in the world.

[0003] According to traditional Chinese medicine, MASLD can be classified into the categories of "liver obstruction (lump)", "flank pain", "accumulation" and other symptoms. At present, it is believed that improper diet, emotional disorder, and improper work and rest are the main causes of MASLD. The liver is yin in nature but uses yang, storing blood and governing the release; the spleen is the foundation of the acquired constitution, governing blood and governing transportation and transformation. Wang Mengying of the Qing Dynasty said: "Too much food will block the spleen, too much leisure will cause spleen stagnation, and spleen qi will be stagnant and less healthy, so the drink will stagnate and accumulate dampness." MASLD patients like to eat fatty and sweet food, or eat too much, which causes the water and grain to not be transformed into essence, but to become phlegm and fat, which hurts the spleen and stomach, causing abnormal transportation and transformation, water and dampness stagnation, and phlegm and turbidity. They accumulate in the liver and cause this disease. Raspberry tastes sweet and sour, is warm in nature, and enters the liver, kidney, and bladder meridians. The Classic of Internal Medicine states: "It is responsible for the five internal organs, and the internal organs are yin. All seeds are solid, and can replenish the middle, and they have the power of acid contraction." The kidney governs water and is responsible for the metabolism of water in the body. It is the central link of water metabolism. The regulation of water can eliminate dampness and strengthen the spleen. When dampness is eliminated and phlegm is eliminated, the liver is smooth. Raspberry is one of the eight flavors of Xinzhe. It mainly contains flavonoids, coumarins, phenolic acids, polysaccharides, alkaloids and trace elements. It has multiple activities such as anti-tumor, anti-oxidation, anti-aging and anti-inflammatory, and is widely used in medicine, food, health food and other fields. Summary of the invention

[0004] In view of the problems existing in the prior art, the purpose of the present invention is to provide an application of raspberry water extract in the preparation of a drug for preventing or treating liver damage, which is specifically achieved through the following technical solutions: Application of raspberry water extract in preparing medicine for preventing or treating liver damage.

[0005] Furthermore, the liver damage is metabolism-related fatty liver disease induced by a high-fat diet.

[0006] Furthermore, the preparation method of the raspberry water extract is as follows: after the raspberries are rinsed with clean water, the raspberries are repeatedly soaked in deionized water, the soaking liquids are combined and boiled, the decoction liquid is filtered and boiled again, and the decoction liquid is concentrated to obtain the raspberry water extract.

[0007] Furthermore, the immersion temperature of the deionized water is 10-30° C., and the immersion time is 30 minutes.

[0008] Furthermore, the decoction temperature is 70-100° C., the decoction time is 30 minutes, and the re-decoction time is 1 hour.

[0009] Furthermore, the raspberry water extract is used to reduce the levels of liver inflammatory factors IL-1β and IL-6.

[0010] Furthermore, the raspberry water extract is used to reduce the protein and RNA expression levels of TNF-α.

[0011] Compared with the prior art, the present invention has the following beneficial effects: 1) The present invention uses raspberry water extract, which is one of the eight new Zhejiang herbs and has both medicinal and edible properties, to treat metabolic-related fatty liver disease. The extraction technology is complete, the extraction repeatability of multiple batches of medicinal materials is good, the medicinal materials comply with the national regulations on the management of traditional Chinese medicines, and the efficacy is long-lasting and stable; 2) The medicinal and edible water extract of the present invention has the characteristics of significant efficacy, safety, reliability, and small toxic and side effects in treating metabolic-related fatty liver disease; 3) From a holistic perspective, multi-component, multi-target intervention in metabolic-related fatty liver disease can significantly reduce plasma alanine aminotransferase, aspartate aminotransferase, and liver tissue triglyceride levels, have significant anti-inflammatory and lipid accumulation inhibitory effects, and have good clinical application prospects; 4) The method of use of the present invention is simple and easy to understand. The use of traditional medicinal and edible water extracts is a new approach to treat metabolic-related fatty liver disease and will have a wide range of uses in the field of treating metabolic-related fatty liver disease. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is the identification diagram of the active ingredients of raspberry water extract; Figure 2 This is the effect of raspberry water extract on oral glucose tolerance and insulin resistance level in mice with metabolic-related fatty liver disease, where ** indicates p<0.01; Figure 3 This is the effect of raspberry water extract on plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver tissue cholesterol (TC), triglyceride (TG) in mice with metabolic-related fatty liver disease, where * indicates p<0.05 and ** indicates p<0.01; Figure 4 This is a graph showing the effect of raspberry water extract on lipid accumulation levels in mice with metabolic-related fatty liver disease; Figure 5 This is a diagram of the main active ingredients of raspberry water extract. DETAILED DESCRIPTION

[0013] The present invention is further described below in conjunction with the accompanying drawings in order to better understand the technical solution.

[0014] Example 1

[0015] The sources of materials and reagents used in the present invention are as follows: raspberries were purchased from the Chinese medicine pharmacy of Zhejiang Chinese Medical University; ALT, AST, TC, and TG detection kits were purchased from Nanjing Jiancheng.

[0016] Drug preparation: The preparation method of the raspberry water extract preparation for treating metabolic-related fatty liver disease of the present invention comprises the following steps: firstly, soaking all the components, then adding the remaining components and boiling; and finally, concentrating the decoction to obtain the preparation. The detailed preferred preparation process is as follows: (1) Soak raspberries in deionized water at 25°C for 30 min.

[0017] (2) Add the drug solution to deionized water and boil at 100°C for 30 minutes.

[0018] (3) Take the decoction, filter it, and boil it for another hour.

[0019] (4) Concentrate the liquid medicine to obtain raspberry water extract.

[0020] The active ingredients of the raspberry water extract prepared in this example were identified as follows: Figure 1 Its main active ingredients are as shown in Figure 5 shown.

[0021] Example 2

[0022] The concentrate of the present invention is illustrated by the following typical test treatment, and the dosage is as follows: A raspberry water extract preparation for treating metabolic-associated fatty liver disease mice has a therapeutic effect on the MASLD mouse model.

[0023] Materials: A compound solution of raspberry water extract for treating mice with metabolic-related fatty liver disease (1 g / kg and 2 g / kg); a high-fat diet (Research Diets, D12492) for modeling metabolic-related fatty liver disease; SPF-grade C57BL / 6 mice.

[0024] Methods: Fifty mice were randomly divided into a normal group, a model group, a low-dose raspberry water extract group, a high-dose raspberry water extract group, and a positive drug group, with 10 mice in each group. The mice were adaptively fed for 7 days. The model was established by a high-fat diet, and raspberry water extract was administered from the third week. Before the end of the experiment, glucose tolerance and insulin resistance tests were performed to detect the blood glucose levels of mice; H&E and Oil Red O staining were used to detect the lipid accumulation level in the liver tissue of mice; biochemical tests of ALT and AST in the plasma of mice, and cholesterol and triglyceride levels in the liver tissue were performed; and the results were statistically analyzed.

[0025] Effects of raspberry water extract on oral glucose tolerance and insulin resistance in mice with metabolic-associated fatty liver disease. Figure 2 As shown: Mice were fed a high-fat diet for 18 weeks to establish the MASLD model. Raspberry water extract concentrate was gavaged from the third week of modeling for 16 consecutive weeks. Raspberry water extract significantly improved oral glucose tolerance and insulin resistance in mice with metabolic-associated fatty liver disease.

[0026] Effects of raspberry water extract on plasma ALT, AST and liver tissue TC, TG in mice with metabolic-related fatty liver disease. Figure 3 As shown: The results of biochemical detection experiments showed that compared with the normal group, the plasma ALT, AST, and liver tissue cholesterol and triglyceride levels of mice in the model group were significantly increased (p < 0.01). Treatment with raspberry water extract could alleviate the MASLD plasma ALT, AST, and liver tissue cholesterol and triglyceride levels (p < 0.01).

[0027] Effects of raspberry aqueous extract on lipid accumulation levels in mice with metabolic-associated fatty liver disease, such as Figure 4 As shown: H&E and Oil Red O staining results showed that compared with the normal group, the liver tissue of the model group mice showed mixed fatty changes of large and small vesicles, and the lipid accumulation in the liver tissue increased. All doses of raspberry water extract can significantly reduce the lipid accumulation in the liver tissue and the level of hepatic steatosis in mice with metabolic-related fatty liver disease.

[0028] Therefore, raspberry water extract has a significant improvement effect on the metabolic-related fatty liver disease mouse model induced by a high-fat diet, while reducing the mouse's glucose metabolism disorder and liver lipid accumulation level, suggesting that this compound has good application prospects for metabolic-related fatty liver disease.

[0029] It can be seen from the above experiments and typical tests that the efficacy of the medicament of the present invention is long-lasting and stable, the medicament of the present invention can be made into various preparations, the preparation method is simple and efficient, and the method of use is simple and easy to understand. The present invention uses traditional water extracts based on Chinese medicine and food homologous drugs, which is a new way to treat metabolic-related fatty liver disease and will have a wide range of uses in the field of treating metabolic-related fatty liver disease.

[0030] The raspberry water extract preparation of the present invention has the characteristics of significant efficacy, safety, reliability, and small toxic and side effects in treating metabolic-related fatty liver disease; starting from the overall perspective, multiple components and multiple targets intervene in metabolic-related fatty liver disease, significantly reduce the plasma ALT, AST and liver tissue cholesterol and triglyceride levels of model mice, reduce liver fatty degeneration and liver lipid accumulation, and have good clinical application prospects. This prescription is based on the theory of traditional Chinese medicine and syndrome differentiation and treatment. Starting from the cause of the disease, MASLD patients like to eat fatty and sweet food, or eat too much, which causes the water and grain to be unable to be transformed into fine particles, but to become phlegm and fat, which hurts the spleen and stomach, causing abnormal transportation and transformation, water and dampness accumulation, phlegm and turbidity, accumulation in the liver, and the disease. Raspberry tastes sweet and sour, is warm in nature, and belongs to the liver, kidney, and bladder meridians. The kidney governs water, is responsible for water metabolism in the body, and is the central link of water metabolism. Water regulation can eliminate dampness and strengthen the spleen, and dampness and phlegm are eliminated, then the liver is smooth. This approach combines clinical experience with the pathogenesis to develop a new raspberry water extract preparation with significant therapeutic effects and few toxic and side effects.

Claims

1. Application of raspberry water extract in the preparation of drugs for preventing or treating liver damage.

2. The use according to claim 1, characterized in that The liver damage is metabolism-related fatty liver disease induced by a high-fat diet.

3. The use according to claim 1, characterized in that The preparation method of the raspberry water extract comprises the following steps: after the raspberries are rinsed with clean water, the raspberries are repeatedly soaked in deionized water, the soaking solutions are combined and boiled, the boiled solution is filtered and boiled again, and the boiled solution is concentrated to obtain the raspberry water extract.

4. The use according to claim 3, characterized in that The immersion temperature of the deionized water is 10-30°C and the immersion time is 30 minutes each time.

5. The use according to claim 3, characterized in that The decoction temperature is 70-100°C, the decoction time is 30 minutes, and the second decoction time is 1 hour.

6. The use according to claim 1, characterized in that The raspberry water extract is used in reducing the levels of liver inflammatory factors IL-1β and IL-6.

7. The use according to claim 1, characterized in that The raspberry water extract is used for reducing the protein and RNA expression levels of TNF-α.

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