Micro-fluidic chip and system for detecting microorganisms in water based on optical fiber sensing

Abstract

The invention relates to a micro-fluidic chip and system for detecting microorganisms in water based on optical fiber sensing, and the micro-fluidic chip comprises a micro-fluidic chip which is internally provided with at least one micro-channel; the detection assembly comprises a detection optical fiber and a detection coating, at least one detection channel is formed in the micro-fluidic chip, the detection optical fiber is arranged in the detection channel, the detection end of the detection optical fiber extends to the communication position of the detection channel and the micro-channel, and the detection coating is fixed to the detection end of the detection optical fiber; at least one light source channel is arranged in the excitation optical fiber, the excitation optical fiber is arranged in the light source channel, and the excitation optical fiber and the detection optical fiber are located on the two opposite sides of the micro-channel respectively. The laser optical fiber is used for providing exciting light for fluorescence detection, the detection coating captures microorganisms flowing through the microchannel and then emits fluorescence signals, the detection optical fiber outputs the fluorescence signals to an external optical receiving system, the microorganisms are detected, the structure needed by detection is simplified, the cost is reduced, and the detection efficiency is improved.

Description

Technical Field

[0001] The present application relates to the field of biological detection technology, and in particular to a microfluidic chip and system for detecting microorganisms in water based on optical fiber sensing. Background Art

[0002] In the fields of environmental monitoring, food safety, medical diagnosis and biosafety, it is crucial to quickly and accurately detect the presence and concentration of microorganisms. Traditional microbial detection methods include culture, immunoassay and molecular biology methods. Although these methods are widely used, they are usually complicated to operate, time-consuming, and lack sensitivity and specificity.

[0003] Among the related technologies, microfluidics has been rapidly developed in the field of microbial detection due to its high efficiency, low cost, and high-throughput analysis. The substance to be detected is combined with fluorescein or other markers, and the change in its fluorescence intensity is used to detect the substance.

[0004] However, traditional microfluidics technology requires the integration of multiple light sources and detectors when detecting fluorescence intensity. It has a relatively complex optical path and high-cost optical components, high actual detection costs, and a complex pipeline adjustment process, resulting in low detection efficiency. Summary of the invention

[0005] The embodiments of the present application provide a microfluidic chip and system for detecting microorganisms in water based on optical fiber sensing, so as to solve the technical problems in the related art of high actual detection cost, complex pipeline adjustment process and low detection efficiency.

[0006] In a first aspect, a microfluidic chip for detecting microorganisms in water based on optical fiber sensing is provided, which comprises:

[0007] A microfluidic chip, wherein the microfluidic chip is provided with at least one microchannel, the microchannel comprising a liquid inlet and a liquid outlet, and the microchannel is used for the flow of a liquid to be detected;

[0008] At least one detection component, the detection component includes a detection optical fiber and a detection coating, the microfluidic chip is provided with at least one detection channel, one end of the detection channel is connected to the side wall of the microchannel, the detection optical fiber is arranged in the detection channel, and the detection end of the detection optical fiber extends to the connection between the detection channel and the microchannel, the detection coating is fixed to the detection end of the detection optical fiber, and the detection optical fiber is connected to an external optical receiving system;

[0009] At least one excitation optical fiber, at least one light source channel is provided in the microfluidic chip, one end of the light source channel is connected to the side wall of the microchannel, the excitation optical fiber is provided in the light source channel, the excitation optical fiber and the detection optical fiber are respectively located on opposite sides of the microchannel, the excitation optical fiber is used to connect to an external laser, and the excitation optical fiber is used to provide the detection optical fiber with excitation light for fluorescence detection; wherein,

[0010] The excitation optical fiber provides excitation light to the detection coating, and the detection coating emits a fluorescent signal after capturing the microorganisms flowing through the microchannel, and the fluorescent signal is output by the detection optical fiber.

[0011] In some embodiments, the detection coating includes biological recognition molecules and fluorescent substances.

[0012] In some embodiments, the biorecognition molecule comprises an antibody or an oligonucleotide.

[0013] In some embodiments, the fluorescent substance includes fluorescein isothiocyanate, rhodamine B, or hydroxycoumarin.

[0014] In some embodiments, the detection coating is fixed to the detection end of the detection optical fiber via a coupling agent.

[0015] In some embodiments, the detection component further includes a protective coating, and the protective coating covers the surface of the detection coating.

[0016] In some embodiments, the number of the detection channels corresponds to the number of the light source channels, and the center lines of the detection channels and the light source channels are collinear, so that the receiving direction of the detection optical fiber and the emitting direction of the excitation optical fiber are collinear.

[0017] In some embodiments, the center lines of the detection channel and the light source channel are perpendicular to the center line of the microchannel, so that the length directions of the detection optical fiber and the excitation optical fiber are perpendicular to the center line of the microchannel.

[0018] In some embodiments, a plurality of detection optical fibers and a plurality of excitation optical fibers are arranged on the side of each of the microchannels, and the detection coatings at the detection ends of the detection optical fibers are of different types, and the plurality of detection optical fibers are arranged at intervals in the length direction of the microchannel.

[0019] The beneficial effects of the technical solution provided by this application include:

[0020] The embodiment of the present application provides a microfluidic chip for detecting microorganisms in water based on optical fiber sensing. During detection, the detection fluid enters the microchannel from the liquid inlet and leaves from the liquid outlet, so that the liquid to be detected flows in the microchannel.

[0021] During the flow of the liquid to be detected, the excitation optical fiber provides the corresponding detection component with excitation light for fluorescence detection. The detection coating of the detection component captures the microorganisms flowing through the microchannel and emits a fluorescence signal. The detection optical fiber outputs the fluorescence signal to the external optical receiving system to realize the detection of specific microorganisms.

[0022] The microfluidic chip has a high degree of integration, a small size, and high sensitivity, and can realize instant feedback and detection of biomarkers. It does not need to integrate a high-cost optical system and a complex optical path structure, and has a low detection cost and improved detection efficiency. The detection optical fiber is used to receive and transmit the optical signal, which improves the stability and accuracy of the signal, thereby improving the accuracy and reliability of the detection results.

[0023] In addition, multiple sets of excitation fibers and detection components allow for simultaneous detection of multiple microorganisms on the same chip, with each detection component focusing on a specific biomarker. This approach improves the efficiency and flexibility of multi-target detection.

[0024] In a second aspect, a microfluidic system for detecting microorganisms in water based on optical fiber sensing is provided, comprising the microfluidic chip for detecting microorganisms in water based on optical fiber sensing as described above.

[0025] Another embodiment of the present application provides a microfluidic system for detecting microorganisms in water based on optical fiber sensing. Since the microfluidic system for detecting microorganisms in water based on optical fiber sensing includes the above-mentioned microfluidic chip for detecting microorganisms in water based on optical fiber sensing, the beneficial effects of the microfluidic system for detecting microorganisms in water based on optical fiber sensing are consistent with the beneficial effects of the above-mentioned microfluidic chip for detecting microorganisms in water based on optical fiber sensing, which will not be repeated here. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0027] Figure 1 A schematic diagram of a microfluidic chip for detecting microorganisms in water based on optical fiber sensing provided in an embodiment of the present application;

[0028] Figure 2 A schematic diagram of a microchannel provided in other embodiments of the present application is provided with

[0029] Figure 3 A schematic diagram of a detection component provided in an embodiment of the present application.

[0030] In the figure: 1, microfluidic chip; 1a, microchannel; 1b, liquid inlet; 1c, liquid outlet; 1d, detection channel; 1e, light source channel; 2, detection component; 21, detection optical fiber; 22, detection coating; 23, protective coating. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0032] The embodiment of the present application provides a microfluidic chip and system for detecting microorganisms in water based on optical fiber sensing, which uses laser optical fiber to provide excitation light for fluorescence detection, uses a detection coating to capture microorganisms flowing through a microchannel and then emits a fluorescence signal, and the detection optical fiber outputs the fluorescence signal to an external optical receiving system to detect specific microorganisms, thereby simplifying the structure required for detection, reducing costs, and improving detection efficiency. The present application solves the technical problems in the related art that the actual detection cost is high, the pipeline adjustment process is complicated, and the detection efficiency is low.

[0033] Reference Figure 1 and Figure 2 A microfluidic chip for detecting microorganisms in water based on optical fiber sensing includes a microfluidic chip 1, at least one set of detection components 2, and at least one laser optical fiber. In this embodiment, the number of detection components 2 corresponds to the number of excitation optical fibers. The excitation optical fiber is used to provide excitation light for fluorescence detection to the corresponding detection component 2. The detection coating 22 of the detection component 2 captures the microorganisms flowing through the microchannel 1a and emits a fluorescence signal. The detection optical fiber 21 outputs the fluorescence signal to an external optical receiving system to detect specific microorganisms.

[0034] Reference Figure 1 and Figure 2 , wherein at least one microchannel 1a is provided in the microfluidic chip 1, and the microchannel 1a includes a liquid inlet 1b and a liquid outlet 1c, and the microchannel 1a is used for the flow of the liquid to be detected. By arranging multiple groups of microchannels 1a, multi-channel analysis and detection can be realized simultaneously, thereby improving the detection efficiency.

[0035] In this embodiment, the microfluidic chip 1 is made of plastic polydimethylsiloxane (PDMS) or polymethyl methacrylate (PMMA). The microchannel 1a can be processed on the microfluidic chip 1 by laser, photolithography and other means. The molding technology of the microfluidic chip 1 is relatively mature and will not be repeated here.

[0036] The detection component 2 includes a detection optical fiber 21 and a detection coating 22. When there are multiple detection components 2, the multiple detection components 2 are arranged at intervals in the flow direction of the liquid to be detected in the microchannel 1a. Multiple detection components 2 can be used to detect multiple microorganisms in the liquid to be detected in the microchannel 1a, and each detection component 2 focuses on a specific biomarker. This method improves the efficiency and flexibility of multi-target detection.

[0037] Reference Figure 1 and Figure 2 At least one detection channel 1d is provided in the microfluidic chip 1, and one end of the detection channel 1d is connected to the side wall of the microchannel 1a. When there are multiple detection components 2, the number of the detection channels 1d corresponds to the number of detection components 2, and the detection channels 1d are arranged at intervals in the flow direction of the liquid to be detected in the microchannel 1a for the installation of the detection components 2.

[0038] Reference Figure 1 and Figure 3 The detection optical fiber 21 is arranged in the detection channel 1d, and the detection end of the detection optical fiber 21 extends to the connection point between the detection channel 1d and the microchannel 1a, and the detection coating 22 is fixed to the detection end of the detection optical fiber 21.

[0039] In this arrangement, the detection coating 22 at the detection end of the detection pipeline is located at the junction of the detection channel 1d and the microchannel 1a, and the detection coating 22 fully contacts the liquid to be detected in the microchannel 1a to ensure that the detection coating 22 captures the microorganisms flowing through the microchannel 1a and emits a fluorescent signal, thereby improving the accuracy and sensitivity of the detection.

[0040] The detection optical fiber 21 is connected to an external optical receiving system. The optical receiving system includes a highly sensitive optical detector and a signal processing unit, such as a photomultiplier tube or a photodiode, to receive and quantify the fluorescence signal. The signal processing unit then converts the collected fluorescence signal into a digital signal, which is processed by a microprocessor and then displayed on a user-friendly interface as the detection result, such as the type and concentration of the microorganism.

[0041] In this way, the fluorescent signal is transmitted through the detection optical fiber 21, which improves the transmission stability and accuracy of the signal, does not require the arrangement of a complex optical system, simplifies the detection structure, and improves the detection efficiency.

[0042] Reference Figure 1 and Figure 2 At least one light source channel 1e is provided in the microfluidic chip 1. The number of light source channels 1e is the same as the number of excitation optical fibers. One end of the light source channel 1e is connected to the side wall of the microchannel 1a, the excitation optical fiber is arranged in the light source channel 1e, and the excitation optical fiber and the detection optical fiber 21 are respectively located on opposite sides of the microchannel 1a.

[0043] The excitation optical fiber is used to connect to an external laser, and is used to provide the detection optical fiber 21 with excitation light for fluorescence detection.

[0044] In this arrangement, the excitation optical fiber provides the detection optical fiber 21 with excitation light for fluorescence detection. Under the environmental conditions of the excitation light, the detection coating 22 captures the microorganisms flowing through the microchannel 1a and emits a fluorescence signal to achieve the detection of the microorganisms.

[0045] In addition, the excitation light passes directly through the microchannel 1a, reducing light scattering and loss. This structure optimizes the light path and improves the efficiency of the overall detection system. At the same time, it reduces the need for complex light path adjustments and high-cost optical components, because the key to the system lies in the optical fiber itself and its integration with the microchannel 1a, thereby simplifying the device structure.

[0046] In this embodiment, the number of detection channels 1d corresponds to the number of light source channels 1e, and the center lines of the detection channels 1d and the light source channels 1e are collinear, so that the receiving direction of the detection optical fiber 21 and the emitting direction of the excitation optical fiber are collinear. In this way, the optical path of the excitation light can be further optimized, and the optical path can be shortened, thereby further reducing the scattering and loss of light.

[0047] Reference Figure 1 and Figure 2 Preferably, the center lines of the detection channel 1d and the light source channel 1e are perpendicular to the center line of the microchannel 1a. So that the length directions of the detection optical fiber 21 and the excitation optical fiber are perpendicular to the center line of the microchannel 1a. At this time, the path of the excitation light is the shortest. The transmission and reception of the optical signal are further optimized, the stability and accuracy of the signal are improved, the detection efficiency is improved, and the detection sensitivity is also improved.

[0048] In this embodiment, the external laser includes a fiber-coupled laser.

[0049] The detection coating 22 includes biorecognition molecules and fluorescent substances. The biorecognition molecules are responsible for capturing specific microorganisms flowing through the microchannel 1a. The biorecognition molecules are combined with the fluorescent substances, and the fluorescent substances emit fluorescent signals when combined with the microorganisms. This enables the capture of microorganisms in the microchannel 1a and the emission of fluorescent signals.

[0050] Specifically, the biorecognition molecule includes an antibody or an oligonucleotide. The fluorescent substance includes fluorescein isothiocyanate, rhodamine B, or hydroxycoumarin.

[0051] For example, in some embodiments, fluorescein isothiocyanate (FITC) is used to detect E. coli, with a laser wavelength of 495 nm and an emission wavelength of 519 nm.

[0052] This FITC is a widely used green fluorescent dye suitable for labeling antibodies and other biomolecules for detecting Escherichia coli through specific antigen-antibody reactions.

[0053] In some embodiments, Rhodamine B is used to detect Staphylococcus aureus, and its laser wavelength is 540 nm and the emission wavelength is 625 nm.

[0054] Rhodamine B is a red fluorescent dye whose spectral characteristics are sufficiently separated from FITC to be used to detect different microorganisms in different detection channels 1d. It is usually used to fluorescently label bacteria in combination with specific antibodies.

[0055] In some embodiments, hydroxycoumarin is used to detect Pseudomonas with an excitation wavelength of 387 nm and an emission wavelength of 460 nm.

[0056] This hydroxycoumarin is a blue fluorescent dye with lower excitation and emission wavelengths than FITC and Rhodamine B, making it an excellent choice for avoiding overlap of fluorescence spectra in multiple labeling experiments. It is suitable for combining with specific antibodies to detect Pseudomonas in water.

[0057] It is important to note that fluorescent substances such as FITC, rhodamine B, and hydroxycoumarins do not have the ability to selectively bind to specific microorganisms by themselves. They are usually used to label antibodies, nucleic acid probes, or other biomolecules that can specifically recognize specific microbial targets. In order for these fluorescent dyes to be effectively used to detect specific microorganisms, they need to be used in combination with biorecognition molecules (such as antibodies or oligonucleotides) that can recognize and bind to specific microbial surface molecules. For example, an antibody against a specific surface protein of E. coli can be used and this antibody can be labeled with FITC to achieve specific fluorescent labeling of E. coli.

[0058] Reference Figure 1 and Figure 3 , wherein the detection coating 22 is fixed to the detection end of the detection optical fiber 21 through a coupling agent. In this embodiment, the detection optical fiber 21 is a silicon-based material, and the coupling agent includes a silane coupling agent.

[0059] In this arrangement, the detection coating 22 is usually fixed to the optical fiber end point by a chemical bonding method, that is, the biorecognition molecule and the fluorescent substance are fixed to the optical fiber end point. This coupling method can form a stable chemical bond to ensure that the detection coating 22 is not washed away by the flowing liquid to be detected under experimental conditions.

[0060] Reference Figure 1 and Figure 2Optionally, the detection component 2 further includes a protective coating 23, which covers the surface of the detection coating 22. In this embodiment, the protective coating 23 is a transparent protective material, preferably a polymer film or a special glass covering layer. Specifically, the protective coating is covered on the surface of the detection coating 22 by coating.

[0061] This arrangement uses physical shielding to protect the detection coating 22, prevent the detection coating 22 from being washed away, and prevent potential physical damage or chemical corrosion to the detection coating 22.

[0062] Furthermore, the scouring effect on the coating can be reduced by optimizing the fluid dynamics layout. Specifically, the flow rate of the fluid to be detected can be slowed down, which can greatly reduce the risk of the detection coating 22 being carried away. For example, the flow rate of the fluid to be detected can be controlled within 50 μL / min.

[0063] In this embodiment, since the detection optical fiber 21 is arranged perpendicular to the microchannel 1a, the detection coating 22 on the detection end of the detection optical fiber 21 directly faces the flushing direction of the fluid to be detected. The detection coating 22 is further prevented from falling off.

[0064] Reference Figure 1 In some embodiments, a plurality of detection optical fibers 21 and a plurality of excitation optical fibers are arranged on the side of each microchannel 1a, and the detection coating 22 at the detection end of each detection optical fiber 21 is of a different type, and the plurality of detection optical fibers 21 are arranged at intervals in the length direction of the microchannel 1a.

[0065] With this arrangement, multiple detection components 2 can respectively detect multiple microorganisms in the liquid to be detected in the microchannel 1a, and each detection component 2 focuses on a specific biomarker. This method improves the efficiency and flexibility of multi-target detection.

[0066] The embodiment of the present application provides a microfluidic chip for detecting microorganisms in water based on optical fiber sensing. During detection, the detection fluid enters the microchannel 1a from the liquid inlet 1b and leaves from the liquid outlet 1c, so that the liquid to be detected flows in the microchannel 1a.

[0067] During the flow of the liquid to be detected, the excitation optical fiber provides the corresponding detection component 2 with excitation light for fluorescence detection. The detection coating 22 of the detection component 2 captures the microorganisms flowing through the microchannel 1a and emits a fluorescence signal, which is then output by the detection optical fiber 21 to an external optical receiving system to detect specific microorganisms.

[0068] The microfluidic chip 1 has a high degree of structural integration, a small size, and high sensitivity, and realizes instant feedback and detection of biomarkers. It does not need to integrate a high-cost optical system and a complex optical path structure, and the detection cost is low and the detection efficiency is improved. The detection optical fiber 21 is used to receive and transmit the optical signal, which improves the stability and accuracy of the signal, thereby improving the accuracy and reliability of the detection result.

[0069] In addition, multiple sets of excitation optical fibers and detection components 2 allow for simultaneous detection of multiple microorganisms on the same chip, with each detection component 2 focusing on a specific biomarker. This approach improves the efficiency and flexibility of multi-target detection.

[0070] In a second aspect, a microfluidic system for detecting microorganisms in water based on optical fiber sensing is provided, comprising the microfluidic chip for detecting microorganisms in water based on optical fiber sensing as described above.

[0071] Another embodiment of the present application provides a microfluidic system for detecting microorganisms in water based on optical fiber sensing. Since the microfluidic system for detecting microorganisms in water based on optical fiber sensing includes the above-mentioned microfluidic chip for detecting microorganisms in water based on optical fiber sensing, the beneficial effects of the microfluidic system for detecting microorganisms in water based on optical fiber sensing are consistent with the beneficial effects of the above-mentioned microfluidic chip for detecting microorganisms in water based on optical fiber sensing, which will not be repeated here.

[0072] In the description of the present application, it should be noted that the terms "upper", "lower", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. Unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to the specific circumstances.

[0073] It should be noted that, in this application, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the presence of other identical elements in the process, method, article or device including the elements.

[0074] The above description is only a specific implementation of the present application, so that those skilled in the art can understand or implement the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest range consistent with the principles and novel features applied for herein.

Claims

1. A microfluidic chip for detecting microorganisms in water based on optical fiber sensing, characterized in that: It includes: A microfluidic chip, wherein the microfluidic chip is provided with at least one microchannel, the microchannel comprising a liquid inlet and a liquid outlet, and the microchannel is used for the flow of a liquid to be detected; At least one detection component, the detection component includes a detection optical fiber and a detection coating, the microfluidic chip is provided with at least one detection channel, one end of the detection channel is connected to the side wall of the microchannel, the detection optical fiber is arranged in the detection channel, and the detection end of the detection optical fiber extends to the connection between the detection channel and the microchannel, the detection coating is fixed to the detection end of the detection optical fiber, and the detection optical fiber is connected to an external optical receiving system; At least one excitation optical fiber, at least one light source channel is provided in the microfluidic chip, one end of the light source channel is connected to the side wall of the microchannel, the excitation optical fiber is provided in the light source channel, the excitation optical fiber and the detection optical fiber are respectively located on opposite sides of the microchannel, the excitation optical fiber is used to connect to an external laser, and the excitation optical fiber is used to provide the detection optical fiber with excitation light for fluorescence detection; wherein, The excitation optical fiber provides excitation light to the detection coating, and the detection coating emits a fluorescent signal after capturing the microorganisms flowing through the microchannel, and the fluorescent signal is output by the detection optical fiber.

2. The microfluidic chip for detecting microorganisms in water based on optical fiber sensing according to claim 1, characterized in that: The detection coating includes biological recognition molecules and fluorescent substances.

3. The microfluidic chip for detecting microorganisms in water based on optical fiber sensing according to claim 2, characterized in that: The biorecognition molecules include antibodies or oligonucleotides.

4. The microfluidic chip for detecting microorganisms in water based on optical fiber sensing according to claim 2, characterized in that: The fluorescent substance includes fluorescein isothiocyanate, rhodamine B, or hydroxycoumarin.

5. The microfluidic chip for detecting microorganisms in water based on optical fiber sensing according to claim 1, characterized in that: The detection coating is fixed to the detection end of the detection optical fiber through a coupling agent.

6. The microfluidic chip for detecting microorganisms in water based on optical fiber sensing according to claim 1, characterized in that: The detection component also includes a protective coating, which covers the surface of the detection coating.

7. The microfluidic chip for detecting microorganisms in water based on optical fiber sensing according to claim 1, characterized in that: The number of the detection channels corresponds to the number of the light source channels, and the center lines of the detection channels and the light source channels are collinear, so that the receiving direction of the detection optical fiber and the emitting direction of the excitation optical fiber are collinear.

8. The microfluidic chip for detecting microorganisms in water based on optical fiber sensing according to claim 7, characterized in that: The center lines of the detection channel and the light source channel are both perpendicular to the center line of the microchannel, so that the length directions of the detection optical fiber and the excitation optical fiber are both perpendicular to the center line of the microchannel.

9. The microfluidic chip for detecting microorganisms in water based on optical fiber sensing according to claim 1 or 8, characterized in that: A plurality of detection optical fibers and a plurality of excitation optical fibers are arranged on the side of each of the microchannels, and the detection coatings at the detection ends of the detection optical fibers are of different types. The plurality of detection optical fibers are arranged at intervals in the length direction of the microchannel.

10. A water microorganism detection system based on optical fiber sensing, characterized in that: A microfluidic chip for detecting microorganisms in water based on optical fiber sensing as described in any one of claims 1 to 9.

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