Plant seed consolidation foundation material and foundation consolidation method

Abstract

The invention discloses a plant seed consolidation foundation material and a foundation consolidation method, and belongs to the technical field of foundation consolidation. The plant seed consolidation foundation material comprises plant enzyme clear liquid and reaction liquid, wherein the plant enzyme clear liquid is prepared by the following steps: immersing seed dry powder into a leaching solution, stirring to form a turbid liquid, standing, and filtering to obtain filter residues and a clear liquid I; and immersing the obtained filter residues into the leaching liquid, stirring, standing, filtering, collecting clear liquid II, and uniformly mixing the clear liquid I and the clear liquid II, thereby obtaining the product. The reaction liquid is a mixed solution of urea and calcium chloride. According to the invention, fewer raw materials are used, the related raw materials do not contain organic chemical solvents such as acetone, consumables such as filter cloth and byproducts such as filter residues can be recycled, and the raw materials are truly renewable and pollution-free in use; the preparation process is simple, centrifugation and other plant enzyme extraction processes are not needed, a large amount of manpower and financial resources can be saved, and large-scale application of the EICP technology is facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of construction, and in particular to a plant seed reinforced foundation material and a foundation reinforcement method. Background Art

[0002] Many plant seeds contain urease, which plays an important role in protein metabolism during germination. Among higher plants, the urease content is particularly high in Cucurbitaceae and Leguminosae. Studies have shown that as seeds germinate, the activity and content of urease gradually decrease. In recent years, people have found that urease can be used to induce calcium carbonate precipitation to reinforce soil (EICP). For example, Almajed et al. reported in "Baseline investigation on enzyme-induced calcium carbonate precipitation" that urease extracted from soybeans can cement loose sand particles into sand columns with an unconfined strength greater than 1 MPa. Subsequently, researchers found that EICP can be used not only for soil reinforcement but also for dust prevention, erosion control, and pollutant control. EICP has the advantages of being environmentally friendly, convenient construction, and low carbon emissions. It is of great significance for the transformation of traditional cement-based high-energy consumption and high-emission construction methods, and has therefore become a current research hotspot.

[0003] However, in the EICP reinforcement process, the consumption of urease is large. The initial research used urease with higher purity, and the monomer enzyme activity was higher. However, due to the complex extraction process, it is necessary to centrifuge and add multiple chemical substances multiple times, such as patent CN104480092A, which leads to high price and high activity loss rate when used in environments with more impurities such as rock and soil. In order to reduce the cost of urease, researchers proposed to extract crude enzymes from plant seeds for EICP. For example, Tirkolaei et al. proposed a three-step method for extracting crude enzymes from soybeans and watermelons in "Crudeurease extract for biocementation". First, the shelled seeds were soaked in phosphate buffer and EDTA and crushed with a homogenizer, filtered and centrifuged at high speed to obtain a crude extract; then the urease in the crude extract was fractionated with acetone at -20°C and centrifuged at high speed at low temperature to obtain a precipitate, and the precipitate was dissolved in the buffer again and allowed to stand for one night and centrifuged again to obtain the supernatant; finally, acetone was added again for fractionation to obtain the crude enzyme. Although this method greatly simplifies the urease extraction process, the use of more acetone in the extraction process may have an impact on the health of construction workers and produce more by-products. In addition, low-temperature centrifugation and other steps will still lead to higher costs when used in field projects. In the article "Multiple-phase enzyme-induced carbonate precipitation (EICP) method for soil improvement", He et al. used calcium sulfate salting out instead of acetone fractionation, and proposed a method of crushing, soaking, salting out, and centrifugal collection of supernatant to prepare soybean crude enzyme, and used it to reinforce Ottawa sand to obtain a sand column with a strength of more than 10MPa. However, the urease solution obtained by this crude extraction method contains more other impurity proteins, which is easy to spoil at room temperature and has a very short storage time (less than 12h). Therefore, freshly extracted urease solution is required for each test to ensure the reinforcement effect. However, for actual engineering applications, extracting a large amount of crude enzyme on site not only requires increasing equipment costs but also increases construction processes. The high cost is not conducive to on-site promotion and application. Summary of the invention

[0004] In order to solve the above technical problems, the purpose of the present invention is to provide a plant seed reinforced foundation material and a foundation reinforcement method to solve the problems in the existing EICP technology, such as the urease extraction step is too complicated, the enzyme solution storage time is short, the construction is complicated, the cost is high, and it is not conducive to large-scale engineering applications.

[0005] The technical solution of the present invention to solve the above technical problems is as follows:

[0006] A plant seed reinforced foundation material, the plant seed reinforced foundation material comprising a plant enzyme serum and a reaction liquid;

[0007] The plant enzyme serum is prepared by the following method:

[0008] (1) immersing the seed dry powder in the leaching solution, stirring to form a suspension, and then letting it stand, filtering to obtain a filter residue and a clear liquid I;

[0009] (2) immersing the obtained filter residue in the leaching liquid again, stirring and then standing, filtering and collecting the clear liquid II, combining the clear liquid I and the clear liquid II and mixing them evenly to obtain;

[0010] The reaction solution is a mixed solution of urea and calcium chloride.

[0011] Furthermore, the seeds include at least one of watermelon seeds, soybeans, sword beans, pumpkin seeds and potatoes.

[0012] Furthermore, the seed dry powder is prepared by the following method:

[0013] First, dry the seeds in the sun until there is no moisture on the surface, then grind them into powder;

[0014] The grinding process temperature is 40-80°C; the particle size of the seed dry powder is 200-400 meshes.

[0015] Furthermore, the leaching liquid is a sodium hypochlorite solution with a mass concentration of 0.05%-0.2%.

[0016] Furthermore, in step (1), the mass ratio of seed dry powder to leaching liquid is 0.5-2:3-7; and the standing time is 2-5h.

[0017] Furthermore, in step (2), the filter residue is repeatedly immersed in the leaching liquid to collect and combine the clear liquid until the mass of the clear liquid II is 8-12 times the mass of the seed dry powder, and the mass ratio of the filter residue to the leaching liquid is 0.5-2:1-3 during each immersion; the standing time is 2-5 hours.

[0018] Furthermore, the urease activity of the plant enzyme serum is ≥10U.

[0019] Furthermore, the mass ratio of urea, calcium chloride and water in the reaction solution is 0.5-2:5-10:17-34.

[0020] A method for strengthening a foundation based on plant seed-reinforced foundation materials comprises the following steps:

[0021] After cleaning the surface of the foundation to be reinforced, inject the plant enzyme solution and reaction solution into the foundation to be reinforced for maintenance.

[0022] Furthermore, the volume ratio of the plant enzyme clear solution to the reaction solution is 1:1-6; the injection method is at least one of alternating injection, alternating spraying, injection after mixing and spraying after mixing; the total volume of the injected solution does not exceed 80% of the pore volume of the foundation to be reinforced.

[0023] Furthermore, the curing time is 7-12h.

[0024] The present invention has the following beneficial effects:

[0025] (1) The raw materials used in the present invention are relatively small, and the raw materials involved do not contain organic chemical solvents such as acetone. Consumables such as filter cloth and by-products such as filter residues can be recycled and reused, thus achieving true renewable and pollution-free use of raw materials.

[0026] (2) The preparation process of the present invention is simple, and there is no need to perform plant enzyme extraction processes such as centrifugation. The preparation method is simple and convenient, which can save a lot of manpower and financial resources and is conducive to the large-scale application of EICP technology.

[0027] (3) The present invention uses sodium hypochlorite solution as the leaching liquid, which can prevent the reproduction of microorganisms in the plant enzyme clear solution and cause the enzyme activity to decrease, greatly prolonging the storage time and having commercial value. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The present invention is a flow chart of the preparation of plant seed reinforced foundation material and foundation reinforcement. DETAILED DESCRIPTION

[0029] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples are only used to explain the present invention and are not used to limit the scope of the present invention. If specific conditions are not specified in the embodiments, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0030] Embodiment 1:

[0031] A method for preparing a plant seed reinforced foundation material, comprising the following steps:

[0032] (1) Soybeans are selected as plant seeds. The plant seeds are first air-dried and dried until there is no moisture on the surface. The air-dried plant seeds are then ground into powder using a grinding machine. The temperature of the dry powder is controlled to be 60° C. during the grinding process. The seed dry powder is sieved to obtain a 300-mesh seed powder.

[0033] (2) First, one part (mass fraction) of seed dry powder is added to five parts (mass fraction) of leaching solution (sodium hypochlorite solution with a mass concentration of 0.1%), stirred to form a suspension, and then allowed to stand for 4 hours. After the suspension is stratified, it is filtered with a filter screen, and a multi-stage filter screen is arranged from top to bottom to obtain a filter residue and a clear liquid I;

[0034] (3) adding one part (by mass) of the filter residue to two parts (by mass) of the leaching solution (sodium hypochlorite solution with a mass concentration of 0.05%), stirring to form a suspension, and then standing for 4 hours. After the suspension is stratified, filtering with a filter screen, using a multi-stage filter screen arranged from top to bottom for filtration and separation, repeatedly treating the filter residue with the leaching solution, and collecting the clear liquid obtained after each treatment until the total mass of the obtained clear liquid is ten times the mass of the seed dry powder, mixing the clear liquids to obtain clear liquid II, and finally mixing clear liquid I and clear liquid II to obtain a plant enzyme clear liquid;

[0035] (4) One part (by mass) of industrial urea and seven parts (by mass) of industrial calcium chloride are dissolved in twenty parts (by mass) of water, and after cooling, a reaction solution is obtained.

[0036] A method for strengthening a foundation based on plant seed-reinforced foundation materials comprises the following steps:

[0037] (a) Clean the surface of the foundation to be reinforced and remove weeds, tree roots and other debris;

[0038] (b) Mix the plant enzyme solution and the reaction solution in a volume ratio of 1:1 and inject them into the cleaned foundation. The total volume injected shall not exceed 80% of the pore volume of the foundation to be reinforced. After the injection is completed, cure for 10 hours;

[0039] (c) Repeat step (b) until the strength of the foundation to be reinforced reaches the required level.

[0040] Embodiment 2:

[0041] A method for preparing a plant seed reinforced foundation material, comprising the following steps:

[0042] (1) Potatoes are selected as plant seeds. The plant seeds are first air-dried and dried until there is no moisture on the surface. The air-dried plant seeds are then ground into powder by a grinding machine. The temperature of the dry powder is controlled to be 60° C. during the grinding process. The seed dry powder is sieved to obtain 300-mesh seed powder.

[0043] (2) First, one part (by mass) of seed dry powder is added to five parts (by mass) of leaching liquid (sodium hypochlorite solution with a mass concentration of 0.05%), stirred to form a suspension, and then allowed to stand for 4 hours. After the suspension is stratified, it is filtered with a filter screen, and a multi-stage filter screen is arranged from top to bottom to obtain a filter residue and a clear liquid I;

[0044] (3) adding one part (by mass) of the filter residue to two parts (by mass) of the leaching liquid (sodium hypochlorite solution with a mass concentration of 0.1%), stirring to form a suspension, and then standing for 4 hours. After the suspension is stratified, filtering with a filter screen, using a multi-stage filter screen arranged from top to bottom for filtration and separation, repeatedly treating the filter residue with the leaching liquid, and collecting the clear liquid obtained after each treatment until the total mass of the obtained clear liquid is ten times the mass of the seed dry powder, mixing the clear liquids to obtain clear liquid II, and finally mixing clear liquid I and clear liquid II to obtain a plant enzyme clear liquid;

[0045] (4) One part (by mass) of industrial urea and seven parts (by mass) of industrial calcium chloride are dissolved in twenty parts (by mass) of water, and after cooling, a reaction solution is obtained.

[0046] A method for strengthening a foundation based on plant seed-reinforced foundation materials comprises the following steps:

[0047] (a) Clean the surface of the foundation to be reinforced and remove weeds, tree roots and other debris;

[0048] (b) Inject the plant enzyme solution and the reaction solution alternately in a volume ratio of 1:1 (first inject the plant enzyme solution, then inject the reaction solution, and inject alternately for 5 cycles) into the cleaned foundation. The total volume injected shall not exceed 80% of the pore volume of the foundation to be reinforced. After the injection is completed, cure for 10 hours;

[0049] (c) Repeat step (b) until the strength of the foundation to be reinforced reaches the required level.

[0050] Embodiment 3:

[0051] A method for preparing a plant seed reinforced foundation material, comprising the following steps:

[0052] (1) Watermelon seeds are selected as plant seeds. The plant seeds are first air-dried and dried until there is no moisture on the surface. Then, the air-dried plant seeds are ground into powder by a grinding machine. The temperature of the dry powder is controlled to be 60° C. during the grinding process. The seed dry powder of 300 mesh is obtained by sieving.

[0053] (2) First, one part (by mass) of seed dry powder is added to five parts (by mass) of leaching liquid (sodium hypochlorite solution with a mass concentration of 0.2%), stirred to form a suspension, and then allowed to stand for 4 hours. After the suspension is stratified, it is filtered with a filter screen, and a multi-stage filter screen is arranged from top to bottom to obtain a filter residue and a clear liquid I;

[0054] (3) adding one part (by mass) of the filter residue to two parts (by mass) of the leaching liquid (sodium hypochlorite solution with a mass concentration of 0.1%), stirring to form a suspension, and then standing for 4 hours. After the suspension is stratified, filtering with a filter screen, using a multi-stage filter screen arranged from top to bottom for filtration and separation, repeatedly treating the filter residue with the leaching liquid, and collecting the clear liquid obtained after each treatment until the total mass of the obtained clear liquid is ten times the mass of the seed dry powder, mixing the clear liquids to obtain clear liquid II, and finally mixing clear liquid I and clear liquid II to obtain a plant enzyme clear liquid;

[0055] (4) One part (by mass) of industrial urea and seven parts (by mass) of industrial calcium chloride are dissolved in twenty parts (by mass) of water, and after cooling, a reaction solution is obtained.

[0056] A method for strengthening a foundation based on plant seed-reinforced foundation materials comprises the following steps:

[0057] (a) Clean the surface of the foundation to be reinforced and remove weeds, tree roots and other debris;

[0058] (b) Mix the plant enzyme solution and the reaction solution in a volume ratio of 1:1 and inject them into the cleaned foundation. The total volume injected shall not exceed 80% of the pore volume of the foundation to be reinforced. After the injection is completed, cure for 10 hours;

[0059] (c) Repeat step (b) until the strength of the foundation to be reinforced reaches the required level.

[0060] Embodiment 4:

[0061] A method for preparing a plant seed reinforced foundation material, comprising the following steps:

[0062] (1) Sword beans are selected as plant seeds. The plant seeds are first air-dried and dried until there is no moisture on the surface. The air-dried plant seeds are then ground into powder by a grinder. The temperature of the dry powder is controlled to be 60° C. during the grinding process. The seed dry powder is sieved to obtain a 300-mesh seed powder.

[0063] (2) First, one part (by mass) of seed dry powder is added to five parts (by mass) of leaching liquid (sodium hypochlorite solution with a mass concentration of 0.05%), stirred to form a suspension, and then allowed to stand for 4 hours. After the suspension is separated into layers, a pressure filter is used to filter and separate to obtain a residue and a clear liquid I;

[0064] (3) adding one part (by mass) of the filter residue to two parts (by mass) of the leaching solution (sodium hypochlorite solution with a mass concentration of 0.1%), stirring to form a suspension, and then standing for 4 hours. After the suspension is stratified, filtering and separating by a pressure filter, repeatedly treating the filter residue with the leaching solution, and collecting the clear liquid obtained after each treatment until the total mass of the obtained clear liquid is ten times the mass of the seed dry powder, mixing the clear liquids to obtain clear liquid II, and finally mixing clear liquid I and clear liquid II to obtain a plant enzyme clear liquid;

[0065] (4) One part (by mass) of industrial urea and seven parts (by mass) of industrial calcium chloride are dissolved in twenty parts (by mass) of water, and after cooling, a reaction solution is obtained.

[0066] A method for strengthening a foundation based on plant seed-reinforced foundation materials comprises the following steps:

[0067] (a) Clean the surface of the foundation to be reinforced and remove weeds, tree roots and other debris;

[0068] (b) Mix the plant enzyme solution and the reaction solution in a volume ratio of 1:1 and inject them into the cleaned foundation. The total volume injected shall not exceed 80% of the pore volume of the foundation to be reinforced. After the injection is completed, cure for 10 hours;

[0069] (c) Repeat step (b) until the strength of the foundation to be reinforced reaches the required level.

[0070] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A plant seed reinforced foundation material, characterized in that: The plant seed reinforced foundation material includes plant enzyme serum and reaction liquid; Wherein, the plant enzyme serum is prepared by the following method: (1) immersing the seed dry powder in the leaching solution, stirring to form a suspension, and then letting it stand, filtering to obtain a filter residue and a clear liquid I; (2) immersing the obtained filter residue in the leaching liquid again, stirring and then standing, filtering and collecting the clear liquid II, combining the clear liquid I and the clear liquid II and mixing them evenly to obtain; The reaction solution is a mixed solution of urea and calcium chloride.

2. The plant seed reinforced foundation material according to claim 1, characterized in that: The seeds include at least one of watermelon seeds, soybeans, sword beans, pumpkin seeds and potatoes.

3. The plant seed reinforced foundation material according to claim 1, characterized in that: The seed dry powder is prepared by the following method: First, dry the seeds in the sun until there is no moisture on the surface, then grind them into powder; The grinding process temperature is 40-80°C; the particle size of the seed dry powder is 200-400 meshes.

4. The plant seed reinforced foundation material according to claim 1, characterized in that: The leaching liquid is a sodium hypochlorite solution with a mass concentration of 0.05%-0.2%.

5. The plant seed reinforced foundation material according to claim 1, characterized in that: In the step (1), the mass ratio of seed dry powder to leaching liquid is 0.5-2:3-7; and the standing time is 2-5 hours.

6. The plant seed reinforced foundation material according to claim 1, characterized in that: In the step (2), the filter residue is repeatedly immersed in the leaching liquid, the clear liquid is collected and combined until the mass of the clear liquid II is 8-12 times the mass of the seed dry powder, and the mass ratio of the filter residue to the leaching liquid is 0.5-2:1-3 during each immersion; the standing time is 2-5 hours.

7. The plant seed reinforced foundation material according to claim 1, characterized in that: The mass ratio of urea, calcium chloride and water in the reaction solution is 0.5-2:5-10:17-34.

8. A method for strengthening a foundation based on the plant seed reinforced foundation material according to any one of claims 1 to 7, characterized in that: The following steps are involved: After cleaning the surface of the foundation to be reinforced, inject the plant enzyme solution and reaction solution into the foundation to be reinforced for maintenance.

9. The method for foundation reinforcement according to claim 8, characterized in that: The volume ratio of the plant enzyme clear solution to the reaction solution is 1:1-6; the injection method is at least one of alternating injection, alternating spraying, injection after mixing and spraying after mixing; the total volume of the injected solution does not exceed 80% of the pore volume of the foundation to be reinforced.

10. The method for foundation reinforcement according to claim 8, characterized in that: The curing time is 7-12 hours.

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