Silk protein-oil reassembled water-dispersible oil capsule and preparation method thereof
By reassembling the oil phase and the aqueous phase and microjet treatment in the preparation of silk protein nanoinclusions, the problems of large inclusion size and poor stability in the prior art are solved, and water-dispersed oil capsules with stable structure and large wrapping amount are achieved, which are suitable for a variety of applications.
Patent Information
- Application Number
- CN202510033782.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-09
- Publication Date
- 2025-04-08
AI Technical Summary
During the preparation process, existing silk protein nanoinclusions require emulsifier-induced assembly, the amount of drug packaging is relatively low, and the prepared inclusions are large in size, which is easy to accumulate layer by layer, resulting in poor batch stability and limited application scenarios.
By mixing the oil phase and the aqueous phase and stirring, the wire protein aggregate and the oil phase are induced to be reassembled to form a multi-chamber structure with stable structure, and the oil phase is stably wrapped in large quantities into the multi-chamber, and the water dispersion is improved by microjet or ultrasonic treatment.
The water-dispersed oil capsules with stable structure and large wrapping amount of silk protein-oil are assembled, which have good water dispersion and are suitable for drug carriers and functional skin care products.
Smart Images
Figure CN119770354A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of cosmetic raw materials, and more specifically to a silk protein-oil reassembled water-dispersible oil capsule and a preparation method thereof. Background Art
[0002] Silk protein aggregates have special hydrophilicity and hydrophobicity, and can interact well with various solvents, so they have gradually become excellent carrier matrix materials. Nanocarriers are an important part of drug controlled release and functional skin care. In recent years, silk protein has been combined with emulsifiers, lecithin, etc. to successfully prepare silk protein nano inclusions with large encapsulation volume, solving the water dispersion problem of various water-insoluble active substances.
[0003] However, during the preparation of silk protein nanoinclusions, emulsifiers are needed to induce silk protein assembly, and the upper limit of drug encapsulation amount is relatively low, which makes it difficult to meet the application requirements of some functional skin care products and drug delivery systems.
[0004] In response to the above problems, technicians have developed a method to use high-crystalline silk protein nanofibers to replace emulsifiers, which improves the high-quality encapsulation of the oil phase. However, the size of the prepared inclusions is 1-10 μm, which is easy to stratify and aggregate in the aqueous solution of silk protein aggregates. Drawing on the above ideas, technicians used high-pressure homogenization to prepare smaller inclusions of ordinary silk protein and oil phase, but the batch and stability of the inclusions in the aqueous phase were poor, and the application scenarios were limited. Therefore, the development of silk protein nano-inclusions with stable structure, large encapsulation volume and good water dispersibility is the key to solving the performance limitations of existing silk protein inclusions, which is conducive to the full application of silk protein in the fields of functional skin care and drug delivery. Summary of the invention
[0005] In view of the shortcomings of the prior art, the object of the present invention is to provide a silk protein-oil reassembled water-dispersible oil capsule and a preparation method thereof, which has the advantages of stable structure, large encapsulation volume and good water dispersibility.
[0006] The above technical objectives of the present invention are achieved through the following technical solutions: a method for preparing a water-dispersible oil capsule of silk protein-oil reassembly, comprising the following steps: S1, obtaining an oil phase and an aqueous phase, mixing the oil phase and the aqueous phase, and stirring evenly to obtain a first mixed solution; S2, performing microfluidization or ultrasonic treatment on the first mixed solution to obtain a target product; The aqueous phase comprises a fibroin aggregate aqueous solution, the concentration of fibroin aggregates in the fibroin aggregate solution is 0.1-6wt%, and the mass ratio of the oil phase to the fibroin aggregate aqueous solution is 1:19-2:1.
[0007] In one embodiment, the beta-sheet content of the fibroin aggregates in the fibroin aggregates is greater than 30%, and the size range is 20-4000 nm.
[0008] In one embodiment, the oil phase includes oil and oil-soluble active ingredients.
[0009] In one embodiment, the oil comprises one or more of vegetable oil, animal oil, mineral oil, and synthetic oil.
[0010] In one embodiment, the aqueous phase further comprises a water-soluble active ingredient, and the water-soluble active ingredient comprises one or more of panthenol, fruit acid, vitamin C, curcumin, ferulic acid, asiatica glycoside and salicylic acid.
[0011] In one embodiment, the stirring rate of S1 is 300-3000 rpm, the stirring time is 1-120 min, the microjet pressure of S2 is 4000-30000 pis, and the number of microjet treatments is 1-10 times.
[0012] A silk protein-oil reassembled water-dispersible oil capsule is prepared by the preparation method of the silk protein-oil reassembled water-dispersible oil capsule.
[0013] In one embodiment, the size of the silk protein-oil reassembled water-dispersible oil capsules ranges from 20 to 2000 nm.
[0014] The application of the silk protein-oil reassembled water-dispersible oil capsule in drug carriers and functional skin care products.
[0015] The above-mentioned silk protein-oil reassembled water-dispersible oil capsule and its preparation method have the following beneficial effects: First, by mixing the water phase and the oil phase and stirring them, the silk fibroin aggregates and the oil phase are induced to reassemble, forming a structurally stable multi-chamber structure, which stably wraps a large amount of the oil phase into the multi-chamber structure and has good water dispersibility; Secondly, by loading active substances with different hydrophilicity into the oil phase or the aqueous solution of silk fibroin aggregates, after stirring, microfluidization and other treatments, the active substances can be stably loaded on the final product, which is beneficial to the application of the active substances in the field of skin care; Third, the entire preparation process is simple and the conditions are mild, which is conducive to large-scale production and future transformation. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 is a flow chart of the steps of the present invention; Figure 2 It is a macroscopic schematic diagram of Example 1, Comparative Example 1, and Comparative Example 2 before microfluidization treatment; Figure 3 are atomic force microscope images of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4; Figure 4 It is a graph of the particle size results of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4; Figure 5 It is a macroscopic schematic diagram of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 after dilution and other treatments. DETAILED DESCRIPTION
[0017] The present invention is described in further detail below in conjunction with the embodiments. It should be noted that the embodiments described below are intended to facilitate the understanding of the present invention and do not have any limiting effect on it. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those of ordinary skill in the art to which the present invention belongs. When there is a conflict, the definitions in this specification shall prevail.
[0018] As used herein, the term "prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
[0019] The conjunction "consisting of excludes any unspecified element, step, or component. If used in a claim, this phrase renders the claim closed-ended so that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the body of a claim rather than immediately following the subject matter, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.
[0020] When amount, concentration or other value or parameter is expressed as range, preferred range or a series of upper preferred value and lower preferred value limit range, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1 to 5", described range should be interpreted as including range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.
[0021] A method for preparing a water-dispersible oil capsule reassembled from silk protein and oil, Figure 1 As shown, the following steps are included: S1, obtaining an oil phase and an aqueous phase, mixing the oil phase and the aqueous phase, and stirring evenly to obtain a first mixed solution; S2, performing microfluidization or ultrasonic treatment on the first mixed solution to obtain a target product; The aqueous phase includes a fibroin aggregate aqueous solution, the concentration of the fibroin aggregates in the fibroin aggregate solution is 0.1-6wt%, and the mass ratio of the oil phase to the fibroin aggregate aqueous solution is 1:19-2:1.
[0022] The hydrophobicity of fibroin aggregates can promote uniform mixing of the oil phase and the aqueous solution of fibroin aggregates, avoiding macroscopic phase separation to achieve initial interaction, and microfluidization and ultrasonic treatment induce mutual self-assembly of the two to form the target product of the multi-cavity structure of fibroin filled with the oil phase. Without the assistance of the oil phase, only smaller fibroin aggregate particles can be formed after the same treatment steps as above. The concentration of fibroin aggregates in the fibroin aggregate solution is preferably 0.3-3wt%, and when the concentration is greater than or equal to 2wt%, the aqueous solution of fibroin aggregates is in a gel state. When the mass ratio of the oil phase to the aqueous solution of fibroin aggregates is 1:19-2:1, the maximum encapsulation amount of the oil phase occupies more than 65% of the target product.
[0023] By mixing the water phase and the oil phase and stirring them, the silk fibroin aggregates and the oil phase are induced to reassemble to form a structurally stable multi-chamber structure, which stably wraps the oil phase in large quantities into the multi-chambers and has good water dispersibility.
[0024] Further, the beta-sheet content of the fibroin aggregates in the fibroin aggregates is greater than 30%, and the size range is 20-4000nm.
[0025] Because silk fibroin aggregates with less than 30% β-pleated structure usually have good hydrophilicity and are difficult to form good interactions with the oil phase, the silk fibroin aggregates used in the present invention are high-crystalline silk fibroin aggregates with a β-pleated structure content of more than 30%, which have good hydrophobicity, structural stability and mechanical properties, so as to better mix and reassemble evenly with the oil phase to avoid rapid stratification.
[0026] Specifically, the oil phase includes fats and oil-soluble active ingredients.
[0027] Because many oil-soluble active substances are in powder form, if the oil-soluble active substances, oils and water phases are directly mixed, the powdered oil-soluble active substances may be difficult to dissolve. Therefore, the oils and oil-soluble active substances are first mixed and stirred evenly, so that the oil-soluble active substances are directly loaded onto the oils to form oils loaded with active substances, and then the water phase is added. After subsequent stirring, microfluidization and other steps, the oil-soluble active substances are stably loaded. The types of active substances are not limited here, and all active substances that can be dissolved in oil are included, such as ceramides, phytosterols, etc.
[0028] Specifically, the oil is one or more of vegetable oil, animal oil, mineral oil, and synthetic oil.
[0029] The present invention can also use organic solvents that are immiscible with water to replace oils and fats, such as octyldodecanol, silicone oil, water-insoluble polyols, petroleum ether, and n-hexane.
[0030] Specifically, the water phase further includes water-soluble active substances, and the water-soluble active substances include one or more of panthenol, fruit acid, vitamin C, curcumin, ferulic acid, asiatica glycoside and salicylic acid.
[0031] Because some water-soluble active substances are solid, if the water-soluble active substances, the aqueous solution of fibroin aggregates and the oil phase are directly mixed, the solid water-soluble active substances may be difficult to dissolve. Therefore, the water-soluble active substances are first mixed with the aqueous solution of fibroin aggregates and stirred evenly, so that the water-soluble active substances are directly loaded on the aqueous solution of fibroin aggregates to form a composite aqueous phase, and then the oil phase is added. After subsequent stirring, microfluidization and other steps, the stable loading of the water-soluble active substances is achieved. Among them, curcumin, ferulic acid, asiatica glycoside and salicylic acid need to be dissolved in polyols first, and then mixed and stirred with the aqueous solution of fibroin aggregates. The types of water-soluble active substances are not limited here, and both water-soluble active substances and alcohol-soluble active substances are included.
[0032] Specifically, the stirring rate of S1 is 300-3000 rpm, the stirring time is 1-120 min, the microjet pressure of S2 is 4000-30000 pis, and the number of microjet treatments is 1-10 times.
[0033] When the water phase and the oil phase are observed to be transformed into a uniform mixture, and no stratification occurs after stopping stirring for ten minutes, it means that the stirring is in place. If the stirring rate and time are too low, the uniform mixing of the fibroin aggregate aqueous solution and the oil phase cannot be achieved. If the stirring rate and time are too high, the aggregation of the two will be too strong, resulting in phase separation or precipitation. In addition to stirring, homogenization can also be performed. The homogenization rate is 500-5000rpm and the homogenization time is 1-240min.
[0034] Microfluidization treatment can be replaced by ultrasonic treatment with a power of 100-900w and a treatment time of 1-30min. When the power of microfluidization or ultrasound is too low and the time is too short, the power is insufficient, and the aqueous solution and oil phase of the fibroin aggregates cannot be assembled to form the target product. When the power of microfluidization or ultrasound is too high and the time is too long, the aggregation effect of the two will be too strong, the size of the target product will be too large, and the water dispersibility will be reduced.
[0035] A silk protein-oil reassembled water-dispersible oil capsule is prepared by the silk protein-oil reassembled water-dispersible oil capsule preparation method.
[0036] Specifically, the size of the silk protein-oil reassembled water-dispersible oil capsules ranges from 20 to 2000 nm.
[0037] The size of the water-dispersible oil capsules of silk protein-oil reassembly prepared by the present invention is much smaller than that of the existing silk protein inclusions, so the structural stability is stronger and it is not easy to be layered and aggregated.
[0038] The application of the above silk protein-oil reassembled water-dispersible oil capsules in drug carriers and functional skin care products.
[0039] After the silk fibroin aggregate aqueous solution and the oil phase form a uniform mixture, the two are induced to reassemble to form an oil capsule with a multi-chamber structure, and the multi-chambers are filled with the oil phase, which has good and stable water dispersibility. Functional skin care products can be developed based on this oil capsule, which has high practical value. Example 1
[0040] This embodiment provides a silk protein-oil reassembled water-dispersible oil capsule, and the specific preparation steps are as follows: S1, mixing 20 g of 2 wt% high-crystalline silk protein nanofiber aggregate gel and 15 g of sea buckthorn fruit oil, and stirring at 1500 rpm for 20 min to obtain a first mixed solution; S2. The first mixed solution is subjected to microfluidization treatment at a pressure of 20,000 pis for three times to obtain water-dispersible oil capsules assembled from silk protein-sea buckthorn fruit oil in a solution state. Example 2
[0041] This embodiment provides a silk protein-oil reassembled water-dispersible oil capsule, and the specific preparation steps are as follows: S1, mixing 20 g of 1 wt% high-crystalline silk protein nanofiber aggregate gel and 10 g of fish oil, and stirring at 2000 rpm for 15 min to obtain a first mixed solution; S2. The first mixed solution is subjected to microfluidization treatment at a pressure of 10,000 pis for 8 times to obtain water-dispersible oil capsules of silk protein-fish oil reassembled in a solution state. Example 3
[0042] This embodiment provides a silk protein-oil reassembled water-dispersible oil capsule, and the specific preparation steps are as follows: S1, mixing 20 g of 4 wt% high-crystalline silk protein nanofiber aggregate gel and 30 g of octyldodecanol, and stirring at 1200 rpm for 30 min to obtain a first mixed solution; S2. The first mixed solution is subjected to ultrasonic treatment at a power of 500 W for 10 min to obtain water-dispersible oil capsules of silk protein-octyldodecanol reassembled in a solution state. Example 4
[0043] This embodiment provides a silk protein-oil reassembled water-dispersible oil capsule, and the specific preparation steps are as follows: S1, mixing 20 g of 2 wt% high-crystalline silk protein nanofiber aggregate gel and 2 g of 5% panthenol, adding 8 g of white meadowfoam seed oil, and homogenizing at 3000 rpm for 10 min to obtain a first mixed solution; S2. The first mixed solution is subjected to microfluidization treatment at a pressure of 25000 pis for 4 times to obtain a water-dispersible oil capsule of silk protein-lillard plant seed oil reassembled with panthenol in a solution state. Example 5
[0044] This embodiment provides a silk protein-oil reassembled water-dispersible oil capsule, and the specific preparation steps are as follows: S1, dissolving 4 g of 10% asiaticoside in 6 g of 1,3-butanediol solution, first mixing with 20 g of 3 wt% high-crystalline silk protein nanofiber aggregate gel, then adding 10 g of jojoba oil, stirring at 2000 rpm for 20 min, to obtain a first mixed solution; S2. The first mixed solution is subjected to microfluidization treatment at a pressure of 25000 pis for 4 times to obtain water-dispersible oil capsules of silk protein-jojoba oil reassembled with asiatica glycoside in a solution state. Example 6
[0045] This embodiment provides a silk protein-oil reassembled water-dispersible oil capsule, and the specific preparation steps are as follows: S1. First, 2 g of ceramide and 10 g of octyldodecanol were mixed, and then 20 g of 3 wt% high-crystalline silk protein nanofiber aggregate gel was added, and the mixture was stirred at 1500 rpm for 30 min to obtain a first mixed solution; S2. The first mixed solution is subjected to microfluidization treatment at a pressure of 20,000 pis for 6 times to obtain a water-dispersible oil capsule of silk protein-octyldodecanol reassembled and encapsulated ceramide in a solution state.
[0046] The present invention provides three comparative examples for performance test comparison of various embodiments.
[0047] Preparation steps of Comparative Example 1: S1, mixing 20 g of a 2 wt% low-crystalline silk protein aqueous solution and 15 g of sea buckthorn fruit oil, and stirring at 1500 rpm for 20 min to obtain a first mixed solution; S2. The first mixed solution was subjected to microfluidization treatment at a pressure of 20,000 pis for 3 times to obtain the target product of Comparative Example 1.
[0048] Preparation steps of Comparative Example 2: S1, mixing 20 g of a 2 wt% high-crystalline silk protein aqueous solution and 15 g of sea buckthorn fruit oil, and stirring at 100 rpm for 20 min to obtain a first mixed solution; S2. The first mixed solution was subjected to microfluidization treatment at a pressure of 20,000 pis for three times to obtain the target product of Comparative Example 2.
[0049] Preparation steps of Comparative Example 3: S1, mixing 20 g of a 2 wt % high-crystalline silk protein aqueous solution and 15 g of purified water, and stirring at 1500 rpm for 20 min to obtain a first mixed solution; S2. The first mixed solution was subjected to microfluidization treatment at a pressure of 20,000 pis for three times to obtain the target product of Comparative Example 3.
[0050] Preparation steps of Comparative Example 4: S1, mixing 20 g of a 2 wt% high-crystalline silk protein aqueous solution and 15 g of sea buckthorn fruit oil, and stirring at 1500 rpm for 20 min to obtain a first mixed solution; S2. The first mixed solution was subjected to microfluidization treatment at a pressure of 2000 pis for 3 times to obtain the target product of Comparative Example 3.
[0051] Macro state analysis: The mixtures before microfluidization treatment of Example 1, Comparative Example 1 and Comparative Example 2 were used as detection raw materials, and macroscopic analysis was performed after being placed at room temperature for 24 hours.
[0052] like Figure 2 As shown, Figure 2 (a), (b), and (c) are macroscopic schematic diagrams of the mixtures of Example 1, Comparative Example 1, and Comparative Example 2 before microfluidization treatment, respectively, wherein Example 1 is a uniform paste without oil phase stratification and precipitation, while Comparative Example 1 and Comparative Example 2 both have obvious water-oil separation phenomenon, and Comparative Example 1 produces a large amount of white silk protein aggregates.
[0053] Morphology test analysis: The products obtained in Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were used as detection raw materials to perform atomic force microscopy test analysis.
[0054] like Figure 3 As shown, Figure 3 (a), (b), (c), (d), and (e) are atomic force microscope images of the products obtained in Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4, respectively. In Example 1, capsules with multi-cavity structures of 200-300 nm in size can be observed, while only oil phase and silk protein nanofibers can be observed in Comparative Example 1. A large number of rod-like structures and oil phases, as well as a small number of capsules, and large-area aggregates can be clearly observed in Comparative Example 2. Evenly dispersed nanofibers with a size of 50-200 nm can be observed in Comparative Example 3. Irregular rod-like aggregates can be observed in Comparative Example 4, indicating that only Example 1 successfully prepared water-dispersible oil capsules with a stable multi-cavity structure of silk protein-oil reassembly.
[0055] Particle size test analysis: The products obtained in Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were used as detection raw materials, and particle size test and analysis were performed using a laser particle size analyzer.
[0056] like Figure 4 As shown, Figure 4 (a), (b), (c), (d), and (e) are the particle size diagrams of the products obtained in Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4, respectively. Among them, Example 1 shows that its particle size is about 350 nm, Comparative Example 1, Comparative Example 2, and Comparative Example 4 all show multiple particle sizes, and there are aggregates of μm level, while Comparative Example 3 shows a small particle size of about 60 nm, indicating that only Example 1 achieves the reassembly of the water phase and the oil phase, and the obtained product is the most stable.
[0057] Stability test analysis: Using the products obtained in Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 as detection raw materials, macroscopic analysis was performed at room temperature.
[0058] Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 were diluted 100 times with purified water and placed at room temperature for 48 hours before macroscopic analysis.
[0059] like Figure 5 As shown, Figure 5(a), (b), (c), (d), and (e) are respectively macroscopic schematic diagrams of Example 1, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4. Among them, Example 1 maintains good water dispersibility without stratification and precipitation, while Comparative Examples 1, 2, and 4 all show stratification, indicating that the oil phase is not fully wrapped, and Comparative Example 3 is a transparent solution, indicating that its size is too small and the reassembly of the oil phase and the water phase is not achieved.
[0060] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.
Claims
1. A method for preparing a water-dispersible oil capsule of silk protein-oil reassembly, characterized in that: The following steps are involved: S1, obtaining an oil phase and an aqueous phase, mixing the oil phase and the aqueous phase, and stirring evenly to obtain a first mixed solution; S2, performing microfluidization or ultrasonic treatment on the first mixed solution to obtain a target product; The aqueous phase comprises a fibroin aggregate aqueous solution, the concentration of fibroin aggregates in the fibroin aggregate solution is 0.1-6wt%, and the mass ratio of the oil phase to the fibroin aggregate aqueous solution is 1:19-2:
1.
2. The method for preparing a water-dispersible oil capsule of silk protein-oil reassembly according to claim 1, characterized in that: The beta-sheet content of the fibroin aggregates in the fibroin aggregates is greater than 30%, and the size range is 20-4000nm.
3. The method for preparing a water-dispersible oil capsule of silk protein-oil reassembly according to claim 1, characterized in that: The oil phase includes fats and oil-soluble active substances.
4. The method for preparing a water-dispersible oil capsule of silk protein-oil reassembly according to claim 3, characterized in that: The oil includes one or more of vegetable oil, animal oil, mineral oil and synthetic oil.
5. The method for preparing a water-dispersible oil capsule of silk protein-oil reassembly according to claim 1, characterized in that: The water phase further comprises water-soluble active substances, and the water-soluble active substances comprise one or more of panthenol, fruit acid, vitamin C, curcumin, ferulic acid, asiatica glycoside and salicylic acid.
6. The method for preparing a water-dispersible oil capsule of silk protein-oil reassembly according to claim 1, characterized in that: The stirring rate of S1 is 300-3000 rpm, the stirring time is 1-120 min, the microjet pressure of S2 is 4000-30000 pis, and the number of microjet treatments is 1-10 times.
7. A water-dispersible oil capsule reassembled from silk protein and oil, characterized in that: The silk protein-oil reassembled water-dispersible oil capsule is prepared according to the method for preparing the silk protein-oil reassembled water-dispersible oil capsule according to any one of claims 1 to 6.
8. The silk protein-oil reassembled water-dispersible oil capsule according to claim 7, characterized in that: The size of the silk protein-oil reassembled water-dispersible oil capsules ranges from 20 to 2000 nm.
9. Use of the silk protein-oil reassembled water-dispersible oil capsule according to claim 7 in drug carriers and functional skin care products.