Anti-sugar probiotic particles and preparation method thereof

Abstract

The invention relates to the technical field of probiotics, in particular to an anti-sugar probiotic particle and a preparation method thereof, in order to improve the life quality of diabetics, when the probiotic particle is prepared, the content of the probiotic particle is strictly limited, and inonotuso bliquus polysaccharide, pediococcus pentosaceus, chromium yeast and soybean peptide are introduced into the probiotic particle; by utilizing the synergistic effect of different components and probiotics, the insulin utilization level of a human body is jointly enhanced, and the improvement of the blood sugar level is inhibited; on the basis, in order to avoid the phenomena that the activity of the granules is reduced and the probiotics are inactivated under the action of gastric acid after the granules are taken, the effective components in the granules are subjected to embedding treatment, and substances such as the probiotics added in the granules are prevented from being digested and inactivated by gastric juice through a multiple embedding technology; but can be released in intestinal tracts, so that an excellent anti-sugar effect is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of probiotics, in particular to anti-sugar probiotic particles and a preparation method thereof. Background Art

[0002] Diabetes is a chronic disease characterized by elevated blood glucose levels. Due to insufficient insulin secretion or insensitivity to insulin, the body is unable to utilize and store glucose in the blood, and further causes lesions or even failure of the eyes, kidneys, limbs, blood vessels, nerves, etc.; and with the improvement of living standards this year, the incidence of type 2 diabetes caused by unhealthy lifestyles, overnutrition and even lack of activity is also increasing worldwide, resulting in a large number of diabetics and seriously affecting their quality of life. Summary of the invention

[0003] The purpose of the present invention is to provide a sugar-resistant probiotic granule and a preparation method thereof, so as to solve the problems raised in the prior art.

[0004] To achieve the above object, the present invention provides the following technical solution: a method for preparing anti-sugar probiotic granules, characterized in that it comprises the following steps: S1. Preparation of modified enteric polyacrylic acid emulsion; S11. Disperse gluconic acid in deionized water, heat to 105-115°C, sterilize at high temperature for 15-20 minutes, cool to room temperature, add 3-(4-aminopyridin-3-yl)acrylate, heat to 78-95°C, reflux with stirring to react for 12-24 hours in a dark environment, remove excess solvent by rotary evaporation, collect and dry the product to obtain acrylated polysaccharide; S12. The emulsifier is dispersed in deionized water, stirred and dispersed uniformly, methacrylic acid, methyl methacrylate, butyl acrylate and acrylated polysaccharide are added thereto, and an initiator is added thereto under nitrogen atmosphere protection. The temperature is raised to 75-85° C., and the reaction is stirred for 4.5-6 hours. Then, a sodium carbonate solution is added thereto to adjust the pH value to 7-7.5 to obtain a modified enteric polyacrylic acid emulsion; S2. Preparing probiotic embedding agent; S21. Add sodium alginate and oxidized dextran to distilled water, stir and mix well, heat to 105-110°C, sterilize at high temperature for 10-15 minutes, and cool to room temperature to obtain an embedding solution; S22. Chaga polysaccharide, Pediococcus pentosaceus, yeast chromium, and soybean peptide are mixed to obtain an anti-sugar mixture, which is added to the embedding solution, stirred and mixed evenly, and then spray-dried. During spraying, the air inlet temperature is controlled to be 120-130°C and the air outlet temperature is 70-80°C. After spray drying, the dry powder is collected to obtain a primary embedding agent; S23. After adding acetic acid to deionized water to prepare a 1wt% glacial acetic acid solution, chitosan is added thereto to make the chitosan concentration 1.5-2wt%, the temperature is raised to 105-115°C, high temperature sterilization is performed for 15-30 minutes, the temperature is cooled to 5-15°C, a primary embedding agent is added thereto, the mixture is stirred for 15-30 minutes, and the mixture is filtered. After the filtered product is collected, it is rinsed again with physiological saline at a temperature of 0-5°C, and dried to obtain a secondary embedding agent; S24. The secondary embedding agent is placed on a centrifugal granulator, and a modified enteric polyacrylic acid emulsion is sprayed thereon to form a modified enteric polyacrylic acid coating on the surface of the secondary embedding agent. After the coating is completed, a probiotic embedding agent is obtained; S3. The probiotic embedding agent and the flavoring agent are mixed evenly, and the mixture is sealed and packed in bags to obtain sugar-resistant probiotic granules.

[0005] Furthermore, in step S11, the mass ratio of gluconic acid to 3-(4-aminopyridin-3-yl)acrylate is 1:(0.8-0.85).

[0006] Furthermore, in step S12, the modified enteric polyacrylic acid emulsion is composed of 0.15-0.4 parts of emulsifier, 8-12 parts of methacrylic acid, 4.5-8 parts of methyl methacrylate, 10-15 parts of butyl acrylate, 3-8 parts of acrylated polysaccharide, 0.1-0.12 parts of initiator and 60-80 parts of deionized water in parts by weight.

[0007] Furthermore, in step S12, the emulsifier is sodium dodecyl sulfate; and the initiator is potassium persulfate.

[0008] Furthermore, in step S21, the mass ratio of distilled water, sodium alginate and oxidized dextran is 100:(2-3.5):(1-1.5).

[0009] Furthermore, in step S22, the anti-sugar mixture is composed of 1-2 parts of Chaga polysaccharide, 0.5-1 parts of Pediococcus pentosaceus, 1.1-1.5 parts of yeast chromium, and 2-3 parts of soybean peptides in parts by weight.

[0010] Furthermore, in step S22, the mass ratio of the anti-sugar mixture to the embedding solution is (1-2):100.

[0011] Furthermore, in step S24, the mass of the modified enteric polyacrylic acid coating is 1.5-3% of the mass of the secondary embedding agent.

[0012] Furthermore, a sugar-resistant probiotic granule is prepared by the above method.

[0013] Compared with the prior art, the present invention has the following beneficial effects: In order to improve the quality of life of diabetics, the present invention prepares a sugar-resistant probiotic granule; when preparing the probiotic granule, the present invention strictly limits the content thereof, and introduces Chaga polysaccharide, Pediococcus pentosaceus, yeast chromium, and soybean peptide into the granule; Chaga polysaccharide is a polysaccharide substance extracted from Chaga fungus, which has the effects of anti-oxidation, reducing inflammatory symptoms caused by type 2 diabetes, and reducing insulin resistance; Pediococcus pentosaceus can effectively inhibit glucosidase, reduce the conversion of starch in food into glucose, thereby reducing the level of blood sugar rise after a meal, and the yeast chromium added in the present application is organic chromium, which is an active component of human glucose tolerance factor, and can synergistically enhance the action of insulin, thereby accelerating the absorption of sugars and lipids in human blood and inhibiting the increase of blood sugar levels; On this basis, in order to prevent the particles from being degraded in activity and inactivating probiotics due to the action of gastric acid after taking, the present invention also performs an embedding treatment on the effective components in the particles, mixes the anti-sugar mixture with alginate polysaccharide and oxidized dextran, and forms a primary embedding agent after spray drying, and further embeds it with a chitosan solution on this basis, and further uses a modified enteric polyacrylic acid emulsion for coating; Furthermore, when preparing the modified enteric polyacrylic acid emulsion, since the probiotic particles prepared in the present application undergo a multi-stage embedding treatment, in order to ensure the dissolution effect in the intestine, the present invention also introduces acrylated polysaccharides into its ingredients when preparing the modified enteric polyacrylic acid emulsion, thereby improving the water absorption of the acrylate coating, making it easier to rupture and release the contents after absorbing water and swelling in the intestine, thereby accelerating the effectiveness of the probiotic particles. DETAILED DESCRIPTION

[0014] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present invention.

[0015] The Chaga polysaccharide used in this application is the commercially available QAD-10377 Chaga polysaccharide; the soybean peptide used is the commercially available QAD-0230 soybean oligopeptide with a molecular weight of 800Da; the Pediococcus pentosaceus used is the PRS200427 Pediococcus pentosaceus freeze-dried powder; Example 1. A method for preparing anti-sugar probiotic granules, comprising the following steps: S1. Preparation of modified enteric polyacrylic acid emulsion; S11. Disperse 1 part of gluconic acid in deionized water by weight, heat to 105°C, sterilize at high temperature for 18 minutes, cool to room temperature, add 0.8 parts of 3-(4-aminopyridin-3-yl)acrylate, heat to 85°C, reflux under stirring for 24 hours in a dark environment, remove excess solvent by rotary evaporation, collect and dry the product to obtain acrylated polysaccharide; S12. Disperse 0.3 parts of sodium dodecyl sulfate emulsifier into 70 parts of deionized water by weight, stir and disperse evenly, add 8 parts of methacrylic acid, 6 parts of methyl methacrylate, 12 parts of butyl acrylate and 3 parts of acrylated polysaccharide, add 0.12 parts of sodium persulfate initiator aqueous solution under nitrogen atmosphere, heat to 85°C, stir and react for 6 hours, add sodium carbonate solution to adjust the pH to 7, and obtain a modified enteric polyacrylic acid emulsion; S2. Preparing probiotic embedding agent; S21. Add 2 parts of sodium alginate and 1 part of oxidized dextran to 100 parts of distilled water by weight, stir and mix well, heat to 105°C, sterilize at high temperature for 15 minutes, and cool to room temperature to obtain an embedding solution; S22. By weight, 1.8 parts of Chaga polysaccharide, 1 part of Pediococcus pentosaceus, 1.4 parts of yeast chromium, and 2.1 parts of soybean peptide were mixed to obtain an anti-sugar mixture. According to the weight percentage, 1.2 parts of the anti-sugar mixture are added to 100 parts of the embedding solution, stirred and mixed evenly, and then spray-dried. During spraying, the air inlet temperature is controlled to be 125°C and the air outlet temperature is controlled to be 70°C. After spray drying, the dry powder is collected to obtain a primary embedding agent; S23. After adding acetic acid to deionized water to prepare a 1wt% glacial acetic acid solution, chitosan was added thereto to make the chitosan concentration 1.5wt%, the temperature was raised to 105°C, high temperature sterilized for 15 minutes, cooled to 5°C, a primary embedding agent was added thereto, stirred and mixed for 15 minutes, filtered, the filtered product was collected, and then rinsed again with physiological saline at a temperature of 4°C, and dried to obtain a secondary embedding agent; S24. The secondary embedding agent is placed on a centrifugal granulator, the speed of the centrifugal granulator is adjusted to 100 rpm, the hot air temperature is 40 ° C, the blast volume is 20 * 20L / min, and a modified enteric polyacrylic acid emulsion is sprayed thereon to form a modified enteric polyacrylic acid coating on the surface of the secondary embedding agent. The coating weight is 3% of the mass of the secondary embedding agent. After the coating is completed, a probiotic embedding agent is obtained; S3. The probiotic embedding agent and the flavoring agent are mixed evenly, and the mixture is sealed and packed in bags to obtain sugar-resistant probiotic granules.

[0016] Example 2. A method for preparing anti-sugar probiotic granules, comprising the following steps: Compared with Example 1, this example changes the ratio of the anti-sugar mixture in step S21; S1. Preparation of modified enteric polyacrylic acid emulsion; S11. Disperse 1 part of gluconic acid in deionized water by weight, heat to 105°C, sterilize at high temperature for 18 minutes, cool to room temperature, add 0.8 parts of 3-(4-aminopyridin-3-yl)acrylate, heat to 85°C, reflux under stirring for 24 hours in a dark environment, remove excess solvent by rotary evaporation, collect and dry the product to obtain acrylated polysaccharide; S12. Disperse 0.3 parts of sodium dodecyl sulfate emulsifier into 70 parts of deionized water by weight, stir and disperse evenly, add 8 parts of methacrylic acid, 6 parts of methyl methacrylate, 12 parts of butyl acrylate and 3 parts of acrylated polysaccharide, add 0.12 parts of sodium persulfate initiator aqueous solution under nitrogen atmosphere, heat to 85°C, stir and react for 6 hours, add sodium carbonate solution to adjust the pH to 7, and obtain a modified enteric polyacrylic acid emulsion; S2. Preparing probiotic embedding agent; S21. Add 2 parts of sodium alginate and 1 part of oxidized dextran to 100 parts of distilled water by weight, stir and mix well, heat to 105°C, sterilize at high temperature for 15 minutes, and cool to room temperature to obtain an embedding solution; S22. By weight, 1.8 parts of Chaga polysaccharide, 1 part of Pediococcus pentosaceus, 1.4 parts of yeast chromium, and 2.1 parts of soybean peptide were mixed to obtain an anti-sugar mixture. According to the weight percentage, 1.2 parts of the anti-sugar mixture are added to 100 parts of the embedding solution, stirred and mixed evenly, and then spray-dried. During spraying, the air inlet temperature is controlled to be 125°C and the air outlet temperature is controlled to be 70°C. After spray drying, the dry powder is collected to obtain a primary embedding agent; S23. After adding acetic acid to deionized water to prepare a 1wt% glacial acetic acid solution, chitosan was added thereto to make the chitosan concentration 1.5wt%, the temperature was raised to 105°C, high temperature sterilized for 15 minutes, cooled to 5°C, a primary embedding agent was added thereto, stirred and mixed for 15 minutes, filtered, the filtered product was collected, and then rinsed again with physiological saline at a temperature of 4°C, and dried to obtain a secondary embedding agent; S24. The secondary embedding agent is placed on a centrifugal granulator, the speed of the centrifugal granulator is adjusted to 100 rpm, the hot air temperature is 40 ° C, the blast volume is 20 * 20L / min, and a modified enteric polyacrylic acid emulsion is sprayed thereon to form a modified enteric polyacrylic acid coating on the surface of the secondary embedding agent. The coating weight is 3% of the mass of the secondary embedding agent. After the coating is completed, a probiotic embedding agent is obtained; S3. The probiotic embedding agent and the flavoring agent are mixed evenly, and the mixture is sealed and packed in bags to obtain sugar-resistant probiotic granules.

[0017] Example 3. A method for preparing anti-sugar probiotic granules, comprising the following steps: Compared with Example 1, this example increases the amount of 3-(4-aminopyridin-3-yl)acrylate added in step S11; S1. Preparation of modified enteric polyacrylic acid emulsion; S11. Disperse 1 part of gluconic acid in deionized water by weight, heat to 105°C, sterilize at high temperature for 18 minutes, cool to room temperature, add 0.85 parts of 3-(4-aminopyridin-3-yl)acrylate, heat to 85°C, reflux under stirring for 24 hours in a dark environment, remove excess solvent by rotary evaporation, collect and dry the product to obtain acrylated polysaccharide; S12. Disperse 0.3 parts of sodium dodecyl sulfate emulsifier into 70 parts of deionized water by weight, stir and disperse evenly, add 8 parts of methacrylic acid, 6 parts of methyl methacrylate, 12 parts of butyl acrylate and 3 parts of acrylated polysaccharide, add 0.12 parts of sodium persulfate initiator aqueous solution under nitrogen atmosphere, heat to 85°C, stir and react for 6 hours, add sodium carbonate solution to adjust the pH to 7, and obtain a modified enteric polyacrylic acid emulsion; S2. Preparing probiotic embedding agent; S21. Add 2 parts of sodium alginate and 1 part of oxidized dextran to 100 parts of distilled water by weight, stir and mix well, heat to 105°C, sterilize at high temperature for 15 minutes, and cool to room temperature to obtain an embedding solution; S22. By weight, 1.8 parts of Chaga polysaccharide, 1 part of Pediococcus pentosaceus, 1.4 parts of yeast chromium, and 2.1 parts of soybean peptide were mixed to obtain an anti-sugar mixture. According to the weight percentage, 1.2 parts of the anti-sugar mixture are added to 100 parts of the embedding solution, stirred and mixed evenly, and then spray-dried. During spraying, the air inlet temperature is controlled to be 125°C and the air outlet temperature is controlled to be 70°C. After spray drying, the dry powder is collected to obtain a primary embedding agent; S23. After adding acetic acid to deionized water to prepare a 1wt% glacial acetic acid solution, chitosan was added thereto to make the chitosan concentration 1.5wt%, the temperature was raised to 105°C, high temperature sterilized for 15 minutes, cooled to 5°C, a primary embedding agent was added thereto, stirred and mixed for 15 minutes, filtered, the filtered product was collected, and then rinsed again with physiological saline at a temperature of 4°C, and dried to obtain a secondary embedding agent; S24. The secondary embedding agent is placed on a centrifugal granulator, the speed of the centrifugal granulator is adjusted to 100 rpm, the hot air temperature is 40 ° C, the blast volume is 20 * 20L / min, and a modified enteric polyacrylic acid emulsion is sprayed thereon to form a modified enteric polyacrylic acid coating on the surface of the secondary embedding agent. The coating weight is 3% of the mass of the secondary embedding agent. After the coating is completed, a probiotic embedding agent is obtained; S3. The probiotic embedding agent and the flavoring agent are mixed evenly, and the mixture is sealed and packed in bags to obtain sugar-resistant probiotic granules.

[0018] Example 4. A method for preparing anti-sugar probiotic granules, comprising the following steps: Compared with Example 1, this example increases the amount of acrylic acid esterified polysaccharide added in step S12; S1. Preparation of modified enteric polyacrylic acid emulsion; S11. Disperse 1 part of gluconic acid in deionized water by weight, heat to 105°C, sterilize at high temperature for 18 minutes, cool to room temperature, add 0.8 parts of 3-(4-aminopyridin-3-yl)acrylate, heat to 85°C, reflux under stirring for 24 hours in a dark environment, remove excess solvent by rotary evaporation, collect and dry the product to obtain acrylated polysaccharide; S12. Disperse 0.3 parts of sodium dodecyl sulfate emulsifier into 70 parts of deionized water by weight, stir and disperse evenly, add 8 parts of methacrylic acid, 6 parts of methyl methacrylate, 12 parts of butyl acrylate and 8 parts of acrylated polysaccharide, add 0.12 parts of sodium persulfate initiator aqueous solution under nitrogen atmosphere, heat to 85°C, stir and react for 6 hours, add sodium carbonate solution to adjust the pH to 7, and obtain a modified enteric polyacrylic acid emulsion; S2. Preparing probiotic embedding agent; S21. Add 2 parts of sodium alginate and 1 part of oxidized dextran to 100 parts of distilled water by weight, stir and mix well, heat to 105°C, sterilize at high temperature for 15 minutes, and cool to room temperature to obtain an embedding solution; S22. By weight, 1.8 parts of Chaga polysaccharide, 1 part of Pediococcus pentosaceus, 1.4 parts of yeast chromium, and 2.1 parts of soybean peptide were mixed to obtain an anti-sugar mixture. According to the weight percentage, 1.2 parts of the anti-sugar mixture are added to 100 parts of the embedding solution, stirred and mixed evenly, and then spray-dried. During spraying, the air inlet temperature is controlled to be 125°C and the air outlet temperature is controlled to be 70°C. After spray drying, the dry powder is collected to obtain a primary embedding agent; S23. After adding acetic acid to deionized water to prepare a 1wt% glacial acetic acid solution, chitosan was added thereto to make the chitosan concentration 1.5wt%, the temperature was raised to 105°C, high temperature sterilized for 15 minutes, cooled to 5°C, a primary embedding agent was added thereto, stirred and mixed for 15 minutes, filtered, the filtered product was collected, and then rinsed again with physiological saline at a temperature of 4°C, and dried to obtain a secondary embedding agent; S24. The secondary embedding agent is placed on a centrifugal granulator, the speed of the centrifugal granulator is adjusted to 100 rpm, the hot air temperature is 40 ° C, the blast volume is 20 * 20L / min, and a modified enteric polyacrylic acid emulsion is sprayed thereon to form a modified enteric polyacrylic acid coating on the surface of the secondary embedding agent. The coating weight is 3% of the mass of the secondary embedding agent. After the coating is completed, a probiotic embedding agent is obtained; S3. The probiotic embedding agent and the flavoring agent are mixed evenly, and the mixture is sealed and packed in bags to obtain sugar-resistant probiotic granules.

[0019] Example 5. A method for preparing anti-sugar probiotic granules, comprising the following steps: Compared with Example 1, this embodiment reduces the total weight proportion of the coating in step S24; S1. Preparation of modified enteric polyacrylic acid emulsion; S11. Disperse 1 part of gluconic acid in deionized water by weight, heat to 105°C, sterilize at high temperature for 18 minutes, cool to room temperature, add 0.8 parts of 3-(4-aminopyridin-3-yl)acrylate, heat to 85°C, reflux under stirring for 24 hours in a dark environment, remove excess solvent by rotary evaporation, collect and dry the product to obtain acrylated polysaccharide; S12. Disperse 0.3 parts of sodium dodecyl sulfate emulsifier into 70 parts of deionized water by weight, stir and disperse evenly, add 8 parts of methacrylic acid, 6 parts of methyl methacrylate, 12 parts of butyl acrylate and 3 parts of acrylated polysaccharide, add 0.12 parts of sodium persulfate initiator aqueous solution under nitrogen atmosphere, heat to 85°C, stir and react for 6 hours, add sodium carbonate solution to adjust the pH to 7, and obtain a modified enteric polyacrylic acid emulsion; S2. Preparing probiotic embedding agent; S21. Add 2 parts of sodium alginate and 1 part of oxidized dextran to 100 parts of distilled water by weight, stir and mix well, heat to 105°C, sterilize at high temperature for 15 minutes, and cool to room temperature to obtain an embedding solution; S22. By weight, 1.8 parts of Chaga polysaccharide, 1 part of Pediococcus pentosaceus, 1.4 parts of yeast chromium, and 2.1 parts of soybean peptide were mixed to obtain an anti-sugar mixture. According to the weight percentage, 1.2 parts of the anti-sugar mixture are added to 100 parts of the embedding solution, stirred and mixed evenly, and then spray-dried. During spraying, the air inlet temperature is controlled to be 125°C and the air outlet temperature is controlled to be 70°C. After spray drying, the dry powder is collected to obtain a primary embedding agent; S23. After adding acetic acid to deionized water to prepare a 1wt% glacial acetic acid solution, chitosan was added thereto to make the chitosan concentration 1.5wt%, the temperature was raised to 105°C, high temperature sterilized for 15 minutes, cooled to 5°C, a primary embedding agent was added thereto, stirred and mixed for 15 minutes, filtered, the filtered product was collected, and then rinsed again with physiological saline at a temperature of 4°C, and dried to obtain a secondary embedding agent; S24. The secondary embedding agent is placed on a centrifugal granulator, the speed of the centrifugal granulator is adjusted to 100 rpm, the hot air temperature is 40 ° C, the blast volume is 20 * 20L / min, and a modified enteric polyacrylic acid emulsion is sprayed thereon to form a modified enteric polyacrylic acid coating on the surface of the secondary embedding agent. The coating weight is 1% of the mass of the secondary embedding agent. After the coating is completed, a probiotic embedding agent is obtained; S3. The probiotic embedding agent and the flavoring agent are mixed evenly, and the mixture is sealed and packed in bags to obtain sugar-resistant probiotic granules.

[0020] Comparative Example 1. A method for preparing anti-sugar probiotic granules, comprising the following steps: Compared with Example 1, this comparative example only carried out step S21; S1. Preparation of probiotic embedding agent; S11. Add 2 parts of sodium alginate and 1 part of oxidized dextran to 100 parts of distilled water by weight, stir and mix well, heat to 105°C, sterilize at high temperature for 15 minutes, and cool to room temperature to obtain an embedding solution; S12. By weight, 1.8 parts of Chaga polysaccharide, 1 part of Pediococcus pentosaceus, 1.4 parts of yeast chromium, and 2.1 parts of soybean peptide were mixed to obtain an anti-sugar mixture. By weight, 1.2 parts of the anti-sugar mixture were added to 100 parts of the embedding solution, stirred and mixed evenly, and then spray-dried. During spraying, the air inlet temperature was controlled to be 125° C. and the air outlet temperature was controlled to be 70° C. After spray drying, the dry powder was collected to obtain probiotic granules; Comparative Example 2. A method for preparing sugar-resistant probiotic granules, comprising the following steps: Compared with Example 1, this comparative example did not prepare a modified enteric polyacrylic acid emulsion and a coating; S1. Preparation of probiotic embedding agent; S11. Add 2 parts of sodium alginate and 1 part of oxidized dextran to 100 parts of distilled water by weight, stir and mix well, heat to 105°C, sterilize at high temperature for 15 minutes, and cool to room temperature to obtain an embedding solution; S12. By weight, 1.8 parts of Chaga polysaccharide, 1 part of Pediococcus pentosaceus, 1.4 parts of yeast chromium, and 2.1 parts of soybean peptide were mixed to obtain an anti-sugar mixture. According to the weight percentage, 1.2 parts of the anti-sugar mixture are added to 100 parts of the embedding solution, stirred and mixed evenly, and then spray-dried. During spraying, the air inlet temperature is controlled to be 125°C and the air outlet temperature is controlled to be 70°C. After spray drying, the dry powder is collected to obtain a primary embedding agent; S13. After adding acetic acid to deionized water to prepare a 1wt% glacial acetic acid solution, chitosan was added thereto to make the chitosan concentration 1.5wt%, the temperature was raised to 105°C, high temperature sterilized for 15 minutes, cooled to 5°C, a primary embedding agent was added thereto, stirred and mixed for 15 minutes, filtered, the filtered product was collected, and then rinsed again with physiological saline at a temperature of 4°C, and dried to obtain a probiotic embedding agent; S2. The probiotic embedding agent and the flavoring agent are mixed evenly, and the mixture is sealed and packed in bags to obtain sugar-resistant probiotic granules.

[0021] Testing: The probiotic granule embedding agents prepared in Examples 1-5 and Comparative Examples 1-2 were tested, and the test results are shown in Table 1 below; 30-day storage survival rate = number of surviving probiotics after 30 days of storage at 4°C in the dark / number of live bacteria before treatment × 100%; Artificial gastric juice and artificial intestinal juice survival rate test; Artificial gastric juice and intestinal juice were prepared. Under a 37° C. environment, 0.1 g of the probiotic granule embedding agent prepared in Example 1-5 and Comparative Example 1-2 was placed in 10 mL of artificial gastric juice and intestinal juice, respectively, and the number of viable bacteria was detected every 1 hour. The rate of viable bacteria dissolved in artificial intestinal fluid = the number of surviving probiotics after artificial intestinal fluid treatment / the number of viable bacteria before treatment × 100%; The rate of viable bacteria dissolved in artificial gastric juice = the number of surviving probiotics after artificial gastric juice treatment / the number of viable bacteria before treatment × 100%; Table 1.

[0022] Test 2: Three-week-old SPF male rats were used as test subjects, and were placed in an environment of 25°C ± 2°C and 55 ± 5% humidity to simulate natural light, with free access to food and water. After one week of culture, they were fed a high-oil and high-fat diet for 3 weeks, and then injected with STZ into the abdominal cavity to induce type 2 diabetes. The probiotic granule embedding agent prepared in Example 1 of the present invention was used to treat them as Example 1. A control group of rats fed with normal saline was set up. All rats were orally administered with an oral dose of 8 g / kg. After 8 weeks of treatment, the mice were subjected to a glucose tolerance test according to a glucose administration dose of 2 g / kg. The test results are shown in Table 2 below; Table 2

[0023] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing anti-sugar probiotic granules, characterized in that: The following steps are involved: S1. Preparation of modified enteric polyacrylic acid emulsion; S11. Disperse gluconic acid in deionized water, heat to 105-115°C, sterilize at high temperature for 15-20 minutes, cool to room temperature, add 3-(4-aminopyridin-3-yl)acrylate, heat to 78-95°C, reflux with stirring to react for 12-24 hours in a dark environment, remove excess solvent by rotary evaporation, collect and dry the product to obtain acrylated polysaccharide; S12. The emulsifier is dispersed in deionized water, stirred and dispersed uniformly, methacrylic acid, methyl methacrylate, butyl acrylate and acrylated polysaccharide are added thereto, and an initiator is added thereto under nitrogen atmosphere protection. The temperature is raised to 75-85° C., and the reaction is stirred for 4.5-6 hours. Then, a sodium carbonate solution is added thereto to adjust the pH value to 7-7.5 to obtain a modified enteric polyacrylic acid emulsion; S2. Preparing probiotic embedding agent; S21. Add sodium alginate and oxidized dextran to distilled water, stir and mix well, heat to 105-110°C, sterilize at high temperature for 10-15 minutes, and cool to room temperature to obtain an embedding solution; S22. Chaga polysaccharide, Pediococcus pentosaceus, yeast chromium, and soybean peptide are mixed to obtain an anti-sugar mixture, which is added to the embedding solution, stirred and mixed evenly, and then spray-dried. During spraying, the air inlet temperature is controlled to be 120-130°C and the air outlet temperature is 70-80°C. After spray drying, the dry powder is collected to obtain a primary embedding agent; S23. After adding acetic acid to deionized water to prepare a 1wt% glacial acetic acid solution, chitosan is added thereto to make the chitosan concentration 1.5-2wt%, the temperature is raised to 105-115°C, high temperature sterilization is performed for 15-30 minutes, the temperature is cooled to 5-15°C, a primary embedding agent is added thereto, the mixture is stirred for 15-30 minutes, and the mixture is filtered. After the filtered product is collected, it is rinsed again with physiological saline at a temperature of 0-5°C, and dried to obtain a secondary embedding agent; S24. The secondary embedding agent is placed on a centrifugal granulator, and a modified enteric polyacrylic acid emulsion is sprayed thereon to form a modified enteric polyacrylic acid coating on the surface of the secondary embedding agent. After the coating is completed, a probiotic embedding agent is obtained; S3. The probiotic embedding agent and the flavoring agent are mixed evenly, and the mixture is sealed and packed in bags to obtain sugar-resistant probiotic granules.

2. The method for preparing the anti-sugar probiotic granules according to claim 1, characterized in that: In step S11, the mass ratio of gluconic acid to 3-(4-aminopyridin-3-yl)acrylate is 1:(0.8-0.85).

3. The method for preparing the sugar-resistant probiotic granules according to claim 1, characterized in that: In step S12, the modified enteric polyacrylic acid emulsion is composed of 0.15-0.4 parts of emulsifier, 8-12 parts of methacrylic acid, 4.5-8 parts of methyl methacrylate, 10-15 parts of butyl acrylate, 3-8 parts of acrylated polysaccharide, 0.1-0.12 parts of initiator and 60-80 parts of deionized water in parts by weight.

4. The method for preparing the anti-sugar probiotic granules according to claim 1, characterized in that: In step S12, the emulsifier is sodium dodecyl sulfate; the initiator is potassium persulfate.

5. The method for preparing the anti-sugar probiotic granules according to claim 1, characterized in that: In step S21, the mass ratio of distilled water, sodium alginate and oxidized dextran is 100:(2-3.5):(1-1.5).

6. The method for preparing the anti-sugar probiotic granules according to claim 1, characterized in that: In step S22, the anti-sugar mixture consists of 1-2 parts of Chaga polysaccharide, 0.5-1 parts of Pediococcus pentosaceus, 1.1-1.5 parts of yeast chromium, and 2-3 parts of soybean peptides, in parts by weight.

7. The method for preparing the sugar-resistant probiotic granules according to claim 1, characterized in that: In step S22, the mass ratio of the anti-sugar mixture to the embedding solution is (1-2):

100.

8. The method for preparing the anti-sugar probiotic granules according to claim 1, characterized in that: In step S24, the mass of the modified enteric polyacrylic acid coating is 1.5-3% of the mass of the secondary embedding agent.

9. An anti-sugar probiotic granule prepared by the preparation method according to any one of claims 1 to 8.

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