Device for separating and extracting fibroin and sericin protein by high-pressure steam and extraction method thereof

Abstract

The device comprises a high-pressure outer cylinder, an outer cylinder sealing cover and an extrusion driving mechanism, an inner cylinder and a heater are arranged in the high-pressure outer cylinder, an inner cylinder sealing cover is arranged on the inner cylinder, and a safety valve is arranged on the inner cylinder sealing cover; the safety valve is automatically opened after the inner cavity of the high-pressure outer cylinder reaches preset pressure so as to communicate the high-pressure outer cylinder with the inner cylinder; the heater is used for heating water to form water vapor; the inner cylinder is provided with a silkworm cocoon processing cavity and a sericin storage cavity, the silkworm cocoon processing cavity and the sericin storage cavity are communicated through a solid-liquid separation channel, the sericin storage cavity is communicated to the outside of the high-pressure outer cylinder through a liquid discharge pipe, and the liquid discharge pipe is provided with a switch valve; the silkworm cocoon processing cavity is provided with an extrusion plate, and the extrusion plate is provided with array type vent holes; the extrusion driving mechanism drives the extrusion plate to extrude the silkworm cocoons subjected to steam treatment in the silkworm cocoon treatment cavity, and the extruded liquid flows to the sericin storage cavity. The method improves the extraction rate of sericin.

Description

Technical Field

[0001] The invention belongs to the technical field of separation and extraction, and particularly relates to a device for separating and extracting silk fibroin and sericin by using high-pressure steam and an extraction method thereof. Background Art

[0002] In the traditional silk degumming process, sericin is removed, while silk fibers with fibroin as the main component are retained for fabric production. Currently, the methods for extracting sericin are mainly divided into physical and chemical methods, among which the most commonly used method is high-temperature hydrolysis. This method can retain the natural structure of sericin, but the recovery rate of sericin in this method is low and is not suitable for large-scale extraction in industry.

[0003] The water vapor high pressure extraction method refers to evaporating water into water vapor and dissolving soluble sericin in water. High pressure can increase the recovery rate of sericin. This method is green, simple, effective, and retains the natural structure of sericin.

[0004] However, the existing water vapor high-pressure extraction method has the following defects: first, the water vapor will be partially condensed during the extraction process, reducing the amount of water vapor and reducing the extraction effect; in addition, the sericin extracted by water vapor still needs to be manually separated, and the sericin will be lost in this process. Summary of the invention

[0005] Based on the above-mentioned shortcomings and deficiencies in the prior art, one of the objects of the present invention is to solve at least one or more of the above-mentioned problems in the prior art. In other words, one of the objects of the present invention is to provide a device for separating and extracting silk fibroin and sericin with high-pressure water vapor and an extraction method thereof that meet one or more of the above-mentioned needs.

[0006] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions:

[0007] A device for separating and extracting silk fibroin and sericin protein by high-pressure water vapor comprises a high-pressure outer cylinder, an outer cylinder sealing cover arranged on the high-pressure outer cylinder, and an extrusion driving mechanism, wherein an inner cylinder and a heater are arranged in the high-pressure outer cylinder, an inner cylinder sealing cover is arranged on the inner cylinder, and a safety valve is arranged on the inner cylinder sealing cover, and the safety valve is automatically opened after the inner cavity of the high-pressure outer cylinder reaches a preset pressure to connect the high-pressure outer cylinder and the inner cylinder; the heater is used to heat water entering the high-pressure outer cylinder to form water vapor; the inner cylinder has a cocoon processing chamber and a sericin protein storage chamber, the cocoon processing chamber is located above the sericin protein storage chamber and is connected through a solid-liquid separation channel, the sericin protein storage chamber is connected to the outside of the high-pressure outer cylinder through a drain pipe, and the drain pipe is provided with a switch valve; wherein the cocoon processing chamber is provided with an extrusion plate, and the extrusion plate has an array of vents; the extrusion driving mechanism penetrates the inner cylinder sealing cover to drive the extrusion plate to extrude the cocoons treated with water vapor in the cocoon processing chamber, and the extruded liquid flows to the sericin protein storage chamber.

[0008] As a preferred solution, a support frame is provided at the bottom of the inner cavity of the high-pressure outer cylinder, and the inner cylinder is provided on the support frame;

[0009] The heater is located in the support frame.

[0010] As a preferred solution, the extrusion drive mechanism is a telescopic air cylinder or a hydraulic telescopic oil cylinder.

[0011] As a preferred solution, the high-pressure outer cylinder is provided with a temperature sensor and a pressure sensor for detecting the temperature and pressure of water vapor in the inner cavity of the high-pressure outer cylinder.

[0012] As a preferred embodiment, the device for separating and extracting silk fibroin and sericin by high-pressure steam also includes a controller and a touch screen, and the touch screen, heater, extrusion drive mechanism, temperature sensor, and pressure sensor are connected to the controller signal.

[0013] As a preferred solution, the high-pressure outer cylinder has a water inlet and a water outlet;

[0014] The water inlet of the high-pressure outer cylinder is connected to a water inlet pipe, and the water inlet pipe is provided with a water inlet valve;

[0015] The water outlet of the high-pressure outer cylinder is connected with a water outlet pipe, and the water outlet pipe is provided with a water outlet valve.

[0016] As a preferred solution, a sealing ring is provided between the outer cylinder sealing cover and the high-pressure outer cylinder, and a sealing ring is provided between the inner cylinder sealing cover and the inner cylinder.

[0017] As a preferred solution, casters are provided at the bottom of the high-pressure outer cylinder.

[0018] The present invention also provides an extraction method of the device for separating and extracting silk fibroin and sericin by high-pressure steam as described in any of the above schemes, comprising the following steps:

[0019] (1) placing silkworm cocoons into the cocoon processing chamber of the inner cylinder, adding water into the high-pressure outer cylinder, and closing the inner cylinder sealing cover and the outer cylinder sealing cover;

[0020] (2) The heater heats the water and evaporates it into water vapor. When the inner cavity of the high-pressure outer cylinder reaches a preset pressure, the safety valve automatically opens, and the water vapor enters the inner cylinder. The extrusion drive mechanism drives the extrusion plate to squeeze up and down, and the extruded liquid flows into the sericin storage cavity through the solid-liquid separation channel. The separation and extraction time is 0.5 to 1 hour.

[0021] (3) After the sericin protein extraction is completed, the sericin protein solution flows out from the drain pipe, and freeze-dried sericin protein is obtained by freeze-drying;

[0022] The sample in the cocoon processing chamber is taken out and rinsed with clean water to obtain silk fibroin.

[0023] As a preferred solution, the preset pressure is 0.1-0.2 MPa, and the heating temperature of the heater is 110-130°C.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. In the traditional high-temperature hydrolysis process, high pressure is not applied, the extraction rate of sericin is low, and silk fibroin and sericin cannot be completely separated. To obtain silk fibroin, other methods need to be used for degumming, which is complicated and inefficient. However, the present invention can control the temperature of the inner cylinder above 100°C through high pressure and heat-conducting inner cylinder, and the safety valve can directly pass high-pressure steam into the inner cylinder, and directly use high temperature and high pressure to extract sericin;

[0026] 2. The traditional high-temperature hydrolysis process cannot directly separate silk fibroin from sericin protein. After the high-temperature hydrolysis, the sericin solution needs to be squeezed out, and there is a lot of loss of sericin protein in this process. However, the sericin protein extracted by water vapor in the present invention is directly squeezed and separated by an extrusion plate, and the sericin protein liquid directly flows into the sericin protein storage chamber through the solid-liquid separation channel. The cocoons after being squeezed can still continue to pass through high-pressure water vapor to continue to extract the residual sericin protein, thereby improving the extraction rate of sericin protein. After the extraction is completed, the sericin protein solution flows out from the drain pipe, and the sericin protein freeze-dried is obtained by freeze-drying; the sample in the cocoon processing chamber is taken out and rinsed with clean water to obtain pure silk fibroin;

[0027] 3. The device of the present invention controls the extraction temperature, pressure and time through the parameter settings of the touch screen, and detects the temperature and pressure through the temperature sensor and the pressure sensor, so that the temperature, pressure and time can be fully controlled;

[0028] 4. The water in the high-pressure outer cylinder of the present invention can be controlled to flow in and out through the water inlet valve and the water outlet valve. When cleaning the inner cylinder, it is only necessary to add clean water to the cocoon processing chamber and open the switch valve of the drain pipe to complete the cleaning, which is simple and easy to operate;

[0029] 5. The device of the present invention is easy to operate, can run continuously, has high efficiency, and the extraction method is green and environmentally friendly, and can be applied to laboratories and industrial production. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a schematic diagram of the structure of the device for separating and extracting silk fibroin and sericin by high-pressure steam according to Example 1 of the present invention. DETAILED DESCRIPTION

[0031] In order to more clearly illustrate the embodiments of the present invention, the specific implementation methods of the present invention will be described below with reference to the accompanying drawings. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, other accompanying drawings and other implementation methods can be obtained based on these accompanying drawings without creative work.

[0032] Embodiment 1:

[0033] like Figure 1 As shown, the device for separating and extracting silk fibroin and sericin by high-pressure steam of this embodiment includes a high-pressure outer cylinder 1, an outer cylinder sealing cover 2, an inner cylinder 3, an inner cylinder sealing cover 4, a hydraulic telescopic cylinder 5, an extrusion plate 6, a heater 7 and a support frame 8.

[0034] The high-pressure outer cylinder 1 of this embodiment is a cylindrical structure, with an opening at the top and covered by an outer cylinder sealing cover 2. A sealing ring 9 is provided between the high-pressure outer cylinder 1 and the outer cylinder sealing cover 2 to improve the sealing performance. Among them, the outer cylinder sealing cover 2 is provided with a controller, a touch screen 10 and an integrated temperature and pressure sensor 11. The probe of the integrated temperature and pressure sensor 11 extends to the inner cavity of the high-pressure outer cylinder 1 to detect the temperature and pressure inside the high-pressure outer cylinder 1. The hydraulic telescopic cylinder 5, the heater 7, the touch screen 10, and the integrated temperature and pressure sensor 11 are all connected to the controller signal to realize intelligent control.

[0035] The support frame 8 of this embodiment is installed at the bottom of the inner cavity of the high-pressure outer cylinder 1, and is used to support and install the inner cylinder 3. The support frame 8 is internally installed with a heater 7, which is used to heat the water in the high-pressure outer cylinder 1 to generate water vapor.

[0036] The inner cylinder 3 of the present embodiment is a cylindrical structure with an opening at the top, and a cocoon processing chamber 3-1, a solid-liquid separation channel 3-3 and a sericin storage chamber 3-2 are sequentially distributed along its axial direction from top to bottom, and the cocoon processing chamber 3-1 is connected with the sericin storage chamber 3-2 through the solid-liquid separation channel 3-3; wherein, the top opening of the inner cylinder 3 is covered by an inner cylinder sealing cover 4, and a sealing ring is provided between the inner cylinder 3 and the outer cylinder sealing cover 2 to improve the sealing performance. In addition, the sericin storage chamber 3-2 is connected to the outside of the high-pressure outer cylinder 1 through a drain pipe 12, and the drain pipe 12 is provided with a switch valve 13; wherein, an extrusion plate 6 is provided in the cocoon processing chamber 3-1, and the extrusion plate 6 is a circular plate structure, and the extrusion plate 6 has an array of vent holes 60; the hydraulic telescopic cylinder 5 is installed in the middle of the outer cylinder sealing cover 2, and the telescopic rod of the hydraulic telescopic cylinder 5 passes through the outer cylinder sealing cover 2, the inner cylinder sealing cover 4 and is connected to the extrusion plate 6, so as to drive the extrusion plate 6 to squeeze the cocoons treated with water vapor in the cocoon processing chamber 3-1, and the squeezed liquid flows to the sericin storage chamber 3-2 through the solid-liquid separation channel 3-3.

[0037] The inner cylinder sealing cover 4 of this embodiment is provided with a safety valve 14 , which is used to automatically open after the inner cavity of the high-pressure outer cylinder reaches a preset pressure to connect the high-pressure outer cylinder 1 with the inner cylinder 3 .

[0038] The water inlet of the high-pressure outer cylinder 1 of this embodiment is connected to a water inlet pipe 15, and the water inlet pipe 15 is provided with a water inlet valve 16 for external water inlet; the water outlet of the high-pressure outer cylinder 1 is connected to a water outlet pipe 17, and the water outlet pipe 17 is provided with a water outlet valve 18 for controlling the water outlet. In addition, casters 19 are provided at the bottom of the high-pressure outer cylinder 1 of this embodiment to facilitate the movement of the entire device.

[0039] The extraction method of the device for separating and extracting silk fibroin and sericin by high-pressure steam of this embodiment comprises the following steps:

[0040] Open the outer cylinder sealing cover and the inner cylinder sealing cover;

[0041] Put the cocoons into the cocoon processing chamber of the inner cylinder, add water into the high-pressure outer cylinder, close the inner cylinder sealing cover and the outer cylinder sealing cover, heat the water in the heater to evaporate into water vapor, and when the inner cavity of the high-pressure outer cylinder reaches the preset pressure, the safety valve automatically opens, and the water vapor enters the inner cylinder. The hydraulic telescopic cylinder drives the extrusion plate to squeeze up and down, and the squeezed liquid flows into the sericin storage chamber through the solid-liquid separation channel. The separation and extraction time is 0.5 to 1 hour; wherein, the preset pressure is 0.1 to 0.2 MPa, and the heating temperature of the heater is 110 to 130°C;

[0042] After the sericin protein extraction is completed, the sericin protein solution flows out from the drain pipe, and freeze-dried sericin protein is obtained by freeze-drying method;

[0043] The sample in the cocoon processing chamber is taken out and rinsed with clean water to obtain silk fibroin;

[0044] The remaining unevaporated water in the high-pressure outer cylinder is discharged from the water outlet pipe.

[0045] The device of this embodiment is used to separate and extract silk fibroin and sericin, and the specific process is as follows:

[0046] 50g of shredded and cleaned silk cocoons were placed into a 10L silk cocoon processing chamber, and 10L of water was added into the water inlet. The parameters of pressure were set to 0.14MPa, temperature to 125℃, and time to 30min. The water was evaporated into steam. After reaching the target time and pressure parameters, the safety valve was opened, and steam was introduced into the silk cocoon processing chamber. At the same time, the squeezing plate began to squeeze up and down, and the squeezed liquid flowed into the sericin storage chamber through the solid-liquid separation channel. After the sericin extraction was completed, the sericin solution flowed out from the drain pipe, and was centrifuged at 6000r / min for 10min, and the supernatant was freeze-dried to obtain freeze-dried sericin. After the sample from the silk cocoon processing chamber was taken out, it was rinsed with clean water and dried to obtain pure silk fibroin, and the remaining water flowed out from the water outlet.

[0047] The recommended parameter settings for the green separation and extraction of silk fibroin and sericin by high-pressure steam are shown in Table 1.

[0048] Table 1 Recommended parameters for separation and extraction of silk fibroin and sericin

[0049]

[0050] The higher the temperature, the higher the extraction efficiency, but too high a temperature may lead to the degradation of sericin. The increase in pressure helps to accelerate the dissolution and diffusion of sericin, but it should be controlled within a suitable range to prevent thermal degradation. The extraction time is closely related to temperature and pressure, and higher temperature and pressure can usually shorten the extraction time.

[0051] Embodiment 2:

[0052] The device for separating and extracting silk fibroin and sericin by high-pressure steam in this embodiment is different from that in Embodiment 1 in that:

[0053] The hydraulic telescopic cylinder is replaced with a telescopic cylinder to meet the needs of different applications;

[0054] For other structures, please refer to Example 1.

[0055] Embodiment 3:

[0056] The device for separating and extracting silk fibroin and sericin by high-pressure steam in this embodiment is different from that in Embodiment 1 in that:

[0057] The cocoon processing chamber and the sericin storage chamber can be designed as a split type, and the two can be connected by a solid-liquid separation pipeline to meet the needs of different applications;

[0058] For other structures, please refer to Example 1.

[0059] The above description is only a detailed description of the preferred embodiments and principles of the present invention. For ordinary technicians in this field, according to the ideas provided by the present invention, there will be changes in the specific implementation methods, and these changes should also be regarded as the protection scope of the present invention.

Claims

1. A device for separating and extracting silk fibroin and sericin by high-pressure steam, characterized in that: The invention comprises a high-pressure outer cylinder, an outer cylinder sealing cover arranged on the high-pressure outer cylinder, and an extrusion driving mechanism. An inner cylinder and a heater are arranged in the high-pressure outer cylinder, an inner cylinder sealing cover is arranged on the inner cylinder, and a safety valve is arranged on the inner cylinder sealing cover. The safety valve is used to automatically open after the inner cavity of the high-pressure outer cylinder reaches a preset pressure to connect the high-pressure outer cylinder and the inner cylinder. The heater is used to heat water entering the high-pressure outer cylinder to form water vapor. The inner cylinder has a cocoon processing chamber and a sericin storage chamber. The cocoon processing chamber is located above the sericin storage chamber and is connected through a solid-liquid separation channel. The sericin storage chamber is connected to the outside of the high-pressure outer cylinder through a drainage pipe, and the drainage pipe is provided with a switch valve. Among them, the cocoon processing chamber is provided with an extrusion plate, and the extrusion plate has an array of vents. The extrusion driving mechanism passes through the inner cylinder sealing cover to drive the extrusion plate to extrude the cocoons treated with water vapor in the cocoon processing chamber, and the extruded liquid flows to the sericin storage chamber.

2. The device for separating and extracting silk fibroin and sericin by high pressure steam according to claim 1, characterized in that: A support frame is provided at the bottom of the inner cavity of the high-pressure outer cylinder, and the inner cylinder is arranged on the support frame; The heater is located in the support frame.

3. The device for separating and extracting silk fibroin and sericin by high pressure steam according to claim 1, characterized in that: The extrusion drive mechanism is a telescopic cylinder or a hydraulic telescopic oil cylinder.

4. The device for separating and extracting silk fibroin and sericin by high pressure steam according to claim 1, characterized in that: The high-pressure outer cylinder is provided with a temperature sensor and a pressure sensor for detecting the temperature and pressure of water vapor in the inner cavity of the high-pressure outer cylinder.

5. The device for separating and extracting silk fibroin and sericin by high pressure steam according to claim 4, characterized in that: It also includes a controller and a touch screen, and the touch screen, a heater, an extrusion drive mechanism, a temperature sensor, and a pressure sensor are connected to the controller signal.

6. The device for separating and extracting silk fibroin and sericin by high pressure steam according to claim 1, characterized in that: The high-pressure outer cylinder has a water inlet and a water outlet; The water inlet of the high-pressure outer cylinder is connected to a water inlet pipe, and the water inlet pipe is provided with a water inlet valve; The water outlet of the high-pressure outer cylinder is connected with a water outlet pipe, and the water outlet pipe is provided with a water outlet valve.

7. The device for separating and extracting silk fibroin and sericin by high pressure steam according to claim 1, characterized in that: A sealing ring is arranged between the outer cylinder sealing cover and the high-pressure outer cylinder, and a sealing ring is arranged between the inner cylinder sealing cover and the inner cylinder.

8. The device for separating and extracting silk fibroin and sericin by high pressure steam according to claim 1, characterized in that: The bottom of the high-pressure outer cylinder is provided with casters.

9. The extraction method of the device for separating and extracting silk fibroin and sericin by high pressure steam according to any one of claims 1 to 8, characterized in that: The following steps are involved: (1) placing silkworm cocoons into the cocoon processing chamber of the inner cylinder, adding water into the high-pressure outer cylinder, and closing the inner cylinder sealing cover and the outer cylinder sealing cover; (2) The heater heats the water and evaporates it into water vapor. When the inner cavity of the high-pressure outer cylinder reaches a preset pressure, the safety valve automatically opens, and the water vapor enters the inner cylinder. The extrusion drive mechanism drives the extrusion plate to squeeze up and down, and the extruded liquid flows into the sericin storage cavity through the solid-liquid separation channel. The separation and extraction time is 0.5 to 1 hour. (3) After the sericin protein extraction is completed, the sericin protein solution flows out from the drain pipe, and freeze-dried sericin protein is obtained by freeze-drying; The sample in the cocoon processing chamber is taken out and rinsed with clean water to obtain silk fibroin.

10. The extraction method according to claim 9, characterized in that The preset pressure is 0.1-0.2 MPa, and the heating temperature of the heater is 110-130°C.

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