A molecular diagnosis pre-processing device and a molecular diagnosis pre-processing method

Abstract

The present invention relates to the field of gene detection technology, and specifically discloses a molecular diagnosis pretreatment device and a molecular diagnosis pretreatment method, in which the opening mechanism includes a clamping assembly for clamping a cover body and a tube fixing assembly for fixing a sample tube, and the clamping end of the clamping assembly can rotate relative to the tube fixing assembly around the axis of the sample tube; the pipette in the cup-dividing mechanism is used to transfer the sample in the sample tube to multiple test tubes; the extraction mechanism includes a beating assembly and an extraction assembly, and the beating sleeve in the beating assembly is slidably arranged on the frame along the axial direction of the test tube, and can reciprocate in the test tube of the extraction station; the magnetic bar in the extraction assembly is slidably arranged on the frame along the axial direction of the test tube, and can enter the beating sleeve or move out of the beating sleeve, and the transmission mechanism is used to transfer the sample tube and the test tube. The above arrangement improves the extraction effect, is not easily contaminated, and improves work efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of gene detection, and in particular to a molecular diagnosis pre-processing device and a molecular diagnosis pre-processing method. Background Art

[0002] When conducting molecular diagnostic testing, before performing the polymerase chain reaction, the collected samples need to undergo a series of processing, including shaking and mixing the samples, opening the lid to separate the liquids, adding various reaction reagents and enzymes, and then pipetting them onto the corresponding consumables and transferring them to specific equipment for nucleic acid extraction.

[0003] Pretreatment involves multiple different instruments and equipment, which are easily contaminated during manual transfer. The accuracy of reagent pipetting depends on the proficiency of the personnel, resulting in poor consistency in sample pretreatment results and easy operational errors that affect subsequent test results. Finally, multiple processes require multiple manual interventions, resulting in low work efficiency and high labor costs. Summary of the invention

[0004] The purpose of the present invention is to provide a molecular diagnosis pretreatment device and a molecular diagnosis pretreatment method to solve the problems in the prior art of easy contamination of sample processing, poor consistency of processing results, low efficiency and high cost.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] A molecular diagnosis pre-processing device, comprising:

[0007] frame;

[0008] The cover opening mechanism comprises a clamping assembly for clamping the cover body and a tube fixing assembly for fixing the sample tube, wherein the clamping end of the clamping assembly can rotate relative to the tube fixing assembly around the axis of the sample tube;

[0009] A cup-splitting mechanism, the cup-splitting mechanism comprising a cup-splitting moving assembly and a pipette provided at an output end of the cup-splitting moving assembly, the pipette being used to transfer the sample in the sample tube to a plurality of test tubes;

[0010] An extraction mechanism, the extraction mechanism includes a flapping assembly and an extraction assembly, the flapping assembly includes a flapping drive and a flapping sleeve, the flapping sleeve is slidably arranged on the frame along the axial direction of the test tube, the flapping drive is arranged on the frame, and is transmission-connected with the flapping sleeve to drive the flapping sleeve to reciprocate in the test tube at the extraction station; the extraction assembly includes an extraction drive and a magnetic rod, the magnetic rod is slidably arranged on the frame along the axial direction of the test tube, the extraction drive is arranged on the frame, and is transmission-connected with the magnetic rod to drive the magnetic rod to enter the flapping sleeve or move out of the flapping sleeve; when the magnetic rod enters the flapping sleeve, the magnetic beads in the test tube are adsorbed on the outer side wall of the flapping sleeve;

[0011] The transmission mechanism is used to move the sample tube from the outside to the cover opening station, and to move the test tube from the cup separation station to the extraction station, and finally move the test tube to the outside.

[0012] As an optional technical solution of a molecular diagnosis pre-processing device, the extraction mechanism includes:

[0013] An extraction base is slidably arranged on the frame along a first direction;

[0014] Extract the first mounting member and slide it on the extraction base along the second direction;

[0015] An extraction slide rail, provided on the extraction first mounting member and extending along a third direction;

[0016] A flapping bracket, the flapping bracket is connected to the flapping driving member and is slidably disposed on the extraction slide rail via a first sliding block, and the flapping sleeve is detachably connected to the flapping bracket;

[0017] A magnetic bracket, the magnetic bracket is connected to the extraction drive member and is slidably disposed on the extraction slide rail via a second slider, and the magnetic rod is disposed on the magnetic bracket;

[0018] and / or,

[0019] The end of the flapping sleeve is a conical structure;

[0020] and / or,

[0021] The end of the magnetic rod is a cone-shaped structure.

[0022] As an optional technical solution of a molecular diagnostic pre-processing device, the clamping assembly includes:

[0023] A clamping base is slidably disposed on the frame along a first direction;

[0024] Clamping the first mounting member, and slidingly disposed on the clamping base along the second direction;

[0025] A second clamping mounting member is slidably disposed on the first clamping mounting member along a third direction;

[0026] A rotating driving member, the rotating driving member is slidably disposed on the clamping second mounting member along a third direction;

[0027] Two elastic members, the two elastic members applying elastic forces in opposite directions to the rotary drive member, so that the rotary drive member is floated on the clamping second mounting member;

[0028] A first clamping driving member, wherein the first clamping driving member is arranged at an output end of the rotating driving member;

[0029] Two cover opening clamping claws are respectively arranged at two output ends of the first clamping driving member so as to be close to or away from each other.

[0030] As an optional technical solution of a molecular diagnostic pre-processing device, the tube fixing assembly includes:

[0031] Tube fixing seat;

[0032] A plurality of pipe fixing members, two adjacent pipe fixing members form a group and are arranged at intervals on the pipe fixing seat and can be close to or away from each other;

[0033] A first screw rod is rotatably mounted on the pipe fixing seat and has at least a plurality of sets of thread segments, each set of thread segments includes driving threads with opposite rotation directions;

[0034] A plurality of connecting flanges, two adjacent connecting flanges form a group, and are respectively connected to two pipe fixing members in the same group, and are respectively matched with two driving threads in the same group of threaded segments;

[0035] The second clamping driving member is arranged on the pipe fixing seat and is drivingly connected with the first screw rod.

[0036] As an optional technical solution of a molecular diagnostic pre-processing device, the cup-dispensing moving assembly includes:

[0037] A cup-dispensing base, slidably disposed on the frame along a first direction;

[0038] A first cup-distributing mounting member is slidably disposed on the cup-distributing base along a second direction;

[0039] A first cup-separating driving member, which is arranged on the first cup-separating mounting member, and an output end of which is drivingly connected to the cup-separating base;

[0040] A second cup-separating mounting part is slidably mounted on the first cup-separating mounting part along a third direction, and the pipette is mounted on the second cup-separating mounting part;

[0041] A second cup-separating driving member, which is disposed on the first cup-separating mounting member, and an output end of which is connected to the second cup-separating mounting member;

[0042] and / or,

[0043] The molecular diagnosis pre-treatment device further comprises a mixing mechanism, and the mixing mechanism comprises:

[0044] A mixing support, arranged on the frame;

[0045] A mixing support, slidably disposed on the mixing support along the second direction;

[0046] The mixing driving member is arranged on the mixing support and is in driving connection with the mixing bearing member to drive the mixing bearing member to move back and forth.

[0047] As an optional technical solution for a molecular diagnostic pretreatment device, the cup moving assembly also includes a cup rack arranged on the cup base along the second direction, and the output end of the first cup driving member is equipped with a cup gear meshing with the cup rack.

[0048] As an optional technical solution for a molecular diagnostic pre-processing device, the transmission mechanism includes:

[0049] A transmission seat is slidably disposed on the cup-distributing base along a second direction;

[0050] A first transmission driving member is arranged on the cup-distributing base, and an output end of the first transmission driving member is connected to the transmission base;

[0051] A transmission first mounting member is slidably disposed on the transmission seat along a third direction;

[0052] The second transmission drive member is arranged on the transmission seat, and the output end is connected to the first transmission mounting member;

[0053] The transmission clamp is arranged on the first transmission mounting part and moves synchronously with the first transmission mounting part to grab and drive the sample tube to move from the first loading station in the rack to the cover opening station.

[0054] As an optional technical solution of a molecular diagnosis pre-treatment device, the molecular diagnosis pre-treatment device further includes a first consumable material storage mechanism, and the first consumable material storage mechanism includes:

[0055] A first consumable base, arranged on the frame;

[0056] A first consumable material carrier, slidably disposed on the first consumable material base along a second direction, and used for containing consumable materials;

[0057] The first consumable driving member is disposed on the first consumable base and is transmission-connected with the first consumable bearing member to drive the first consumable bearing member to move to the cup separation station in the frame and outside the frame.

[0058] As an optional technical solution for a molecular diagnostic pre-processing device, the transmission mechanism includes:

[0059] A first transmission belt, used for moving the sample tube from the outside to a first loading position in the rack along a first direction;

[0060] The second transmission belt is used to move the test tube from the first transmission belt to the second loading station along the first direction and then move the test tube from the second loading station to the outside of the frame.

[0061] A molecular diagnosis pretreatment method, applicable to the molecular diagnosis pretreatment device described in any of the above technical solutions, comprises the following steps:

[0062] The sample tube is transferred to the cover opening station of the rack; the sample tube is fixed by the tube fixing assembly, and the cover body is clamped and rotated by the clamping assembly to remove the cover body from the sample tube;

[0063] The sample in the sample tube is transferred to the test tubes at multiple cupping stations by the cupping mechanism, and the test tube is moved to the extraction station by the transmission mechanism;

[0064] The sample in the test tube is tapped by the tapping sleeve of the tapping assembly to fully mix the magnetic beads with the sample;

[0065] The magnetic rod is inserted into the flapping sleeve in the test tube to adsorb the magnetic beads to the outer wall of the flapping sleeve, and then the flapping sleeve together with the magnetic rod is pulled out of the test tube.

[0066] The present invention has at least the following beneficial effects:

[0067] The present invention provides a molecular diagnosis pretreatment device and a molecular diagnosis pretreatment method. The molecular diagnosis pretreatment device comprises a frame and a cover opening mechanism, a cup separation mechanism, an extraction mechanism and a transmission mechanism arranged on the frame, wherein the cover opening mechanism is used to open the cover of a sample tube; the cup separation mechanism is used to separate and transfer the sample in the sample tube into a plurality of test tubes; the extraction mechanism can beat the sample in the test tube through a beating sleeve so that the sample is violently agitated to improve the extraction effect of nucleic acid molecules; the transmission mechanism transfers the sample tube and the test tube between various workstations, and the whole process is in the same device and is not easily contaminated; at the same time, the degree of manual intervention is reduced, the work efficiency is improved, and the consistency of detection is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0068] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the contents of the embodiments of the present invention and these drawings without paying any creative work.

[0069] Figure 1 It is a schematic diagram of the structure of a molecular diagnosis pre-processing device in an embodiment of the present invention;

[0070] Figure 2 This is a schematic structural diagram of a first-view of one of the sub-supports of the molecular diagnosis pre-treatment device in an embodiment of the present invention;

[0071] Figure 3 This is a schematic structural diagram of a second viewing angle of one of the sub-supports of the molecular diagnosis pre-treatment device in an embodiment of the present invention;

[0072] Figure 4 Schematic diagram of the structure of the mixing mechanism in the embodiment of the present invention;

[0073] Figure 5 It is a structural schematic diagram of a clamping assembly in a first viewing angle in an embodiment of the present invention;

[0074] Figure 6 is a structural schematic diagram of a clamping assembly from a second viewing angle in an embodiment of the present invention;

[0075] Figure 7 It is a structural schematic diagram of a pipe fixing assembly in an embodiment of the present invention;

[0076] Figure 8 It is a schematic diagram of a partial structure of a pipe fixing assembly in an embodiment of the present invention;

[0077] Fig. 9 It is a schematic structural diagram of a first screw rod, a connecting flange and a coupling in an embodiment of the present invention;

[0078] Fig.10 This is a structural schematic diagram of the cup-dispensing mechanism from a first viewing angle in an embodiment of the present invention;

[0079] Fig.11 2 is a structural schematic diagram of the cup-dispensing mechanism according to the embodiment of the present invention from a second viewing angle;

[0080] Fig.12 This is a schematic diagram of the structure of the first consumable material storage mechanism in an embodiment of the present invention;

[0081] Fig.13 It is a schematic diagram of the partial structure of the transmission mechanism in an embodiment of the present invention;

[0082] Fig.14It is a structural schematic diagram of another sub-branch of the molecular diagnosis pre-treatment device in an embodiment of the present invention from a first viewing angle;

[0083] Fig.15 It is a structural schematic diagram of another sub-branch of the molecular diagnosis pre-treatment device according to an embodiment of the present invention from a second viewing angle;

[0084] Fig.16 It is a structural schematic diagram of the extraction mechanism in an embodiment of the present invention;

[0085] Fig.17 It is a partial structural schematic diagram of the extraction mechanism in an embodiment of the present invention;

[0086] Fig.18 It is a cross-sectional schematic diagram of the extraction mechanism in an embodiment of the present invention;

[0087] Fig.19 It is a structural schematic diagram of the second consumable material storage mechanism in an embodiment of the present invention;

[0088] Fig. 20 Schematic diagram of the structure of the third consumables storage mechanism in an embodiment of the present invention.

[0089] In the figure:

[0090] 100, rack;

[0091] 200, clamping assembly; 210, clamping base; 220, clamping first mounting member; 230, clamping second mounting member; 231, clamping third driving member; 240, rotating driving member; 250, first clamping driving member; 260, cover opening clamp; 270, code scanner;

[0092] 300, pipe fixing assembly; 310, pipe fixing seat; 320, pipe fixing member; 330, first screw rod; 340, connecting flange; 350, second clamping driving member; 360, coupling;

[0093] 400, cup-dividing mechanism; 410, cup-dividing base; 420, first cup-dividing mounting member; 430, first cup-dividing driving member; 440, second cup-dividing mounting member; 450, second screw rod; 460, second nut; 470, second cup-dividing driving member; 480, cup-dividing rack; 490, pipette;

[0094] 500, extraction mechanism; 510, flapping assembly; 511, flapping driving member; 512, flapping sleeve; 5121, flapping body; 5122, flapping part; 520, extraction assembly; 521, extraction driving member; 522, magnetic rod; 530, extraction base; 540, extraction first mounting member; 550, extraction slide rail; 560, flapping bracket; 570, magnetic bracket;

[0095] 600, transmission mechanism; 610, transmission seat; 620, transmission first mounting member; 630, transmission first driving member; 640, third screw rod; 650, transmission second driving member; 660, third nut; 670, transmission clamp; 680, transmission base; 681, first transmission belt; 682, second transmission belt; 683, stopper; 684, stopper driving member;

[0096] 710, mixing mechanism; 711, mixing support; 712, mixing bearing member; 713, mixing driving member;

[0097] 720, first consumable material storage mechanism; 721, first consumable material base; 722, first consumable material carrier; 723, first consumable material driving member;

[0098] 730, second consumable material storage mechanism; 731, second consumable material base; 732, second consumable material carrier; 733, second consumable material driving member; 734, heating member;

[0099] 740, third consumable material storage mechanism; 741, third consumable material base; 742, third consumable material carrier; 743, third consumable material driving member; 744, refrigeration module;

[0100] 750. Air filtering mechanism; 760. Shell; 770. Grasping mechanism. DETAILED DESCRIPTION

[0101] Before any embodiments of the application are explained in detail, it is to be understood that the application is not limited in its application to the details of construction and the arrangement of components set forth in the following description or illustrated in the above drawings.

[0102] In this application, the terms "comprises", "includes", "has" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device that includes a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of more restrictions, an element defined by the sentence "comprises a ..." does not exclude the presence of other identical elements in the process, method, article or device that includes the element.

[0103] In this application, the term "and / or" is a description of the association relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this application generally indicates that the related objects before and after are in an "and / or" relationship.

[0104] In the present application, the terms "connect", "combine", "couple", and "install" may refer to direct connection, combination, coupling, or installation, or indirect connection, combination, coupling, or installation. For example, direct connection refers to two parts or components being connected together without the need for an intermediate piece, and indirect connection refers to two parts or components being connected to at least one intermediate piece respectively, and the two parts or components being connected via the intermediate piece. In addition, "connect" and "couple" are not limited to physical or mechanical connections or couplings, and may include electrical connections or couplings.

[0105] In the present application, it will be understood by those of ordinary skill in the art that relative terms (e.g., "about," "approximately," "substantially," etc.) used in conjunction with quantities or conditions include the values ​​and have the meaning indicated by the context. For example, the relative terms include at least the degree of error associated with the measurement of a specific value, the tolerances caused by manufacturing, assembly, and use associated with a specific value, and the like. Such terms should also be considered to disclose a range defined by the absolute values ​​of the two endpoints. Relative terms may refer to plus or minus a certain percentage (e.g., 1%, 5%, 10% or more) of the indicated value. Numerical values ​​that do not use relative terms should also be disclosed as specific values ​​with tolerances. In addition, "substantially" may refer to plus or minus a certain degree (e.g., 1 degree, 5 degrees, 10 degrees or more) on the basis of the indicated angle when expressing a relative angular position relationship (e.g., substantially parallel, substantially perpendicular).

[0106] In this application, it will be understood by those skilled in the art that the function performed by a component can be performed by one component, multiple components, one part, or multiple parts. Similarly, the function performed by a part can also be performed by one part, one component, or a combination of multiple parts.

[0107] In the present application, the terms "upper", "lower", "left", "right", "front", "back" and other directional words are described based on the orientation and positional relationship shown in the accompanying drawings, and should not be understood as limiting the embodiments of the present application. In addition, in the context, it is also necessary to understand that when it is mentioned that an element is connected to another element "upper" or "lower", it can not only be directly connected to another element "upper" or "lower", but also indirectly connected to another element "upper" or "lower" through an intermediate element. It should also be understood that directional words such as upper side, lower side, left side, right side, front side, back side, etc. not only represent the positive orientation, but can also be understood as the lateral orientation. For example, the bottom can include directly below, lower left, lower right, lower front, and lower back, etc.

[0108] like Figures 1 to 20As shown, this embodiment provides a molecular diagnosis pretreatment device, which includes a frame 100, a cover opening mechanism, a cup separation mechanism 400, an extraction mechanism 500 and a transmission mechanism 600. Among them, the cover opening mechanism includes a clamping assembly 200 for clamping the cover body and a tube fixing assembly 300 for fixing the sample tube, and the clamping end of the clamping assembly 200 can rotate around the axis of the sample tube relative to the tube fixing assembly 300; the cup separation mechanism 400 includes a cup separation moving assembly and a pipette 490 arranged at the output end of the cup separation moving assembly, and the pipette 490 is used to transfer the sample in the sample tube to multiple test tubes; the extraction mechanism 500 includes a slapping assembly 510 and an extraction assembly 520, and the slapping assembly 510 includes a slapping driving member 511 and a slapping sleeve 512, and the slapping sleeve 512 is slidably arranged on the frame 100 along the axial direction of the test tube, and the slapping driving member 511 is arranged on the frame 100 and is connected to the slapping driving member 511. The beating sleeve 512 is transmission-connected to drive the beating sleeve 512 to reciprocate in the test tube at the extraction station; the extraction assembly 520 includes an extraction drive 521 and a magnetic rod 522, the magnetic rod 522 is slidably arranged on the frame 100 along the axial direction of the test tube, the extraction drive 521 is arranged on the frame 100, and is transmission-connected to the magnetic rod 522 to drive the magnetic rod 522 to enter the beating sleeve 512 or move out of the beating sleeve 512; when the magnetic rod 522 enters the beating sleeve 512, the magnetic beads in the test tube are adsorbed on the outer wall of the beating sleeve 512; the transmission mechanism 600 is used to move the sample tube from the outside to the lid opening station, and to move the test tube from the cupping station to the extraction station, and finally move the test tube to the outside.

[0109] The extraction mechanism 500 in the molecular diagnosis pretreatment device can beat the sample in the test tube through the beating sleeve 512 so that the sample is violently stirred to improve the extraction effect; the transmission mechanism 600 transfers the sample tube and the test tube between various workstations, and the entire process is in the same device and is not easily contaminated; at the same time, the degree of manual intervention is reduced, work efficiency is improved, and the consistency of detection is improved.

[0110] When the carrier with the sample tube is transported to the inside of the device, the sample in the sample tube needs to be mixed first, and then divided into each test tube through the pipette 490. Specifically, the molecular diagnostic pretreatment device also includes a mixing mechanism 710, and the mixing mechanism 710 includes a mixing bracket 711, a mixing carrier 712 and a mixing drive 713. Among them, the mixing bracket 711 is arranged on the frame 100, and the mixing carrier 712 is slidably arranged on the mixing bracket 711 along the second direction; the mixing drive 713 is arranged on the mixing bracket 711, and is transmission-connected with the mixing carrier 712 to drive the mixing carrier 712 to reciprocate. The reciprocating mixing carrier 712 can mix the samples in the sample tube evenly, and after the mixed samples are divided into each test tube, the content of nucleic acid molecules in each test tube is roughly the same.

[0111] After the sample is mixed, the cover of the sample tube needs to be opened first. Regarding the cover opening mechanism, in some embodiments, the clamping assembly 200 includes a clamping base 210, a first clamping mounting member 220, a second clamping mounting member 230, a rotating driving member 240, a first clamping driving member 250, two elastic members and two cover opening jaws 260. Among them, the clamping base 210 is slidably arranged on the frame 100 along the first direction; the first clamping driving member 250 is arranged on the frame 100 and is in transmission connection with the clamping base 210. The first clamping mounting member 220 is slidably arranged on the clamping base 210 along the second direction; the second clamping driving member is arranged on the clamping base 210 and is in transmission connection with the first clamping mounting member 220. The second clamping mounting member 230 is slidably arranged on the first clamping mounting member 220 along the third direction; the third clamping driving member 231 is arranged on the first clamping mounting member 220 and is in transmission connection with the second clamping mounting member 230. The rotating driving member 240 is arranged to slide along the third direction on the clamping second mounting member 230; two elastic members apply elastic forces in opposite directions to the rotating driving member 240, that is, one elastic member applies an upward elastic force to the rotating driving member 240, and the other elastic member applies a downward elastic force to the rotating driving member 240, so that the rotating driving member 240 is floated and arranged on the clamping second mounting member 230; the first clamping driving member 250 is arranged at the output end of the rotating driving member 240; two opening jaws 260 are respectively arranged at the two output ends of the first clamping driving member 250 to move closer to or away from each other. Among them, the clamping third driving member 231 drives the clamping second mounting member 230 to move up and down through the screw nut assembly. Among them, the first direction is the left-right direction, the second direction is the front-back direction, and the third direction is the up-down direction.

[0112] Specifically, the rotary drive member 240 is connected to the moving block, the moving hole of the moving block is penetrated through the moving rod that clamps the second mounting member 230, and can move up and down along the moving rod. The elastic member is a spring, and is sleeved on the moving rod. The lower end of the spring located above the moving block abuts the moving block, and the upper end is relatively fixed to the clamping second mounting member 230; the upper end of the spring located below the moving block abuts the moving block, and the lower end is relatively fixed to the clamping second mounting member 230.

[0113] The above arrangement enables the cover opening jaws 260 to move freely within the space to match the various test tubes on the cover opening station. After clamping the cover body, the elastic member can be arranged to make the cover body and the sample tube move away from or toward each other, so that the screwing between the two is smoother.

[0114] In addition, the clamping assembly 200 can also be used as a transfer mechanism for the test tube, and cooperates with the transmission mechanism 600 to transfer the sample tube to the cover opening station.

[0115] The pipe fixing assembly 300 includes a pipe fixing seat 310, a plurality of pipe fixing members 320, a plurality of first screw rods 330, a plurality of connecting flanges 340 and a second clamping driving member 350. The pipe fixing seat 310 is provided on the frame 100. Two adjacent pipe fixing members 320 form a group and are arranged at intervals on the pipe fixing seat 310, and the two pipe fixing members 320 in the same group can approach or move away from each other; the first screw rod 330 is rotatably arranged on the pipe fixing seat 310 and has at least a plurality of groups of thread segments, each group of thread segments includes driving threads with opposite rotation directions; two adjacent connecting flanges 340 form a group and are respectively connected to the two pipe fixing members 320 in the same group, and are correspondingly matched with the two driving threads in the thread segments in the same group; the second clamping driving member 350 is provided on the pipe fixing seat 310 and is transmission-connected to the first screw rod 330, and the adjacent first screw rods 330 are connected by a coupling 360.

[0116] The above arrangement enables the two tube fixing members 320 to move synchronously, thereby improving the clamping efficiency of the test tube; wherein, the connecting flange 340 can be made of standard parts with low cost.

[0117] When in use, the two opening jaws 260 are driven closer to each other under the action of the first clamping drive member 250 to clamp the cover of the sample tube, and the two tube fixing members 320 in the same group are driven closer to each other under the action of the second clamping drive member 350 to clamp the sample tube; then the second mounting member 230 is clamped to rise, so that the spring under the rotating drive member 240 is compressed, and the rotating drive member 240 drives the first clamping drive member 250 to rotate, so that the cover and the sample tube are gradually separated, and the compressed spring gradually extends, thereby driving the rotating drive member 240 to rise, so as to drive the cover away from the sample tube, thereby improving the smoothness of separation of the cover and the sample tube and the convenience of control.

[0118] Each sample tube has a marking part such as a QR code or a barcode for marking, wherein the clamping assembly 200 further includes a barcode scanner 270; the barcode scanner 270 is fixed to the clamping first mounting member 220. The shooting direction of the barcode scanner 270 is the first direction. When the cover opening jaw 260 clamps the cover body and the tube fixing assembly 300 does not clamp the sample tube, the rotating driving member 240 rotates, which can drive the sample tube to rotate as a whole, thereby rotating the marking part on the sample tube, so that there is an angle that can face the barcode scanner 270 and be scanned by the barcode scanner 270.

[0119] There are a plurality of cover-opening clamping jaws 260 , which are arranged at intervals along the second direction. Accordingly, the number of the tube fixing members 320 is arranged corresponding to the number of the cover-opening clamping jaws 260 .

[0120] After opening the cover, the sample in the sample tube needs to be divided into each test tube.

[0121] Regarding the transportation of each test tube, in some embodiments, the molecular diagnostic pre-treatment device further includes a first consumable storage mechanism 720, which includes a first consumable base 721, a first consumable carrier 722, and a first consumable drive 723. The first consumable base 721 is provided on the rack 100; the first consumable carrier 722 is slidably provided on the first consumable base 721 along the second direction, and is used to hold consumables; the first consumable drive 723 is provided on the first consumable base 721, and is transmission-connected with the first consumable carrier 722, so as to drive the first consumable carrier 722 to move between the cupping station in the rack 100 and outside the rack 100. The consumables held in the first consumable carrier 722 are test tubes.

[0122] The cup-separating moving assembly includes a cup-separating base 410 , a first cup-separating mounting member 420 , a first cup-separating driving member 430 , a second cup-separating mounting member 440 , a second screw rod 450 , a second nut 460 and a second cup-separating driving member 470 . Among them, the cup base 410 is slidably arranged on the frame 100 along the first direction; the first cup mounting member 420 is slidably arranged on the cup base 410 along the second direction; the first cup driving member 430 is arranged on the first cup mounting member 420, and its output end is transmission-connected with the cup base 410; the second cup mounting member 440 is slidably arranged on the first cup mounting member 420 along the third direction, and the pipette 490 is arranged on the second cup mounting member 440; the second screw rod 450 is rotationally arranged on the first cup mounting member 420; the second nut 460 is rotationally matched with the second screw rod 450 and connected to the second cup mounting member 440; the second cup driving member 470 is arranged on the first cup mounting member 420 and rotationally connected with the second screw rod 450. This arrangement realizes the movement of the pipette 490 at each station within the spatial range.

[0123] Furthermore, the cup moving assembly further includes a cup rack 480 disposed on the cup base 410 along the second direction, and the output end of the first cup driving member 430 is provided with a cup gear meshing with the cup rack 480. This arrangement achieves the accuracy of the movement of the first cup mounting member 420.

[0124] In some embodiments, the transmission mechanism 600 includes a transmission seat 610 , a transmission first mounting member 620 , a transmission first driving member 630 , a third screw rod 640 , a third nut 660 , a transmission second driving member 650 and a transmission clamp 670 . Among them, the transmission seat 610 is slidably arranged on the cup-distributing base 410 along the second direction; the first transmission driving member 630 is arranged on the cup-distributing base 410, and its output end is transmission-connected with the transmission seat 610; the first transmission mounting member 620 is slidably arranged on the transmission seat 610 along the third direction; the third screw rod 640 is rotationally arranged on the first transmission mounting member 620; the third nut 660 is rotationally matched with the third screw rod 640 and is connected with the first transmission mounting member 620; the second transmission driving member 650 is arranged on the transmission seat 610 and is rotationally connected with the third screw rod 640; the transmission clamp 670 is arranged on the first transmission mounting member 620 and moves synchronously with it to grab and drive the carrier carrying the sample tube to move from the first loading station in the rack 100 to the cover opening station, and can move the carrier carrying the test tube from the cup-distributing station to the first loading station. It should be noted that each carrier can hold multiple sample tubes or test tubes. In this embodiment, a transmission driving wheel is provided at the output end of the first transmission driving member 630 , a transmission driven wheel is rotatably arranged on the transmission seat 610 , a transmission belt is wound between the transmission driving wheel and the transmission driven wheel, and is connected to the first transmission mounting member 620 .

[0125] The above-mentioned arrangement can realize pipetting and drive the transmission clamp 670 to move in a spatial range at the same time, so as to move the carrier carrying the test tube to the transmission mechanism 600, and finally move it to the extraction station; at the same time, the cup-dispensing base 410 drives the transmission clamp 670 and the pipette 490 to move at the same time, and the two processes do not conflict with each other, thereby reducing the number of mechanisms driven along the first direction and making the overall structure compact; in addition, only the first-direction driving structure of the cup-dispensing base 410 can be moved to a more marginal position, so that the movement range of the pipette 490 and the transmission clamp 670 in the first direction is larger.

[0126] When the test tube is moved to the extraction station, the nucleic acid molecules inside the sample need to be extracted. In some embodiments, the extraction mechanism 500 also includes an extraction base 530, an extraction first mounting member 540, an extraction slide rail 550, a beating bracket 560, and a magnetic bracket 570. Among them, the extraction base 530 is slidably disposed on the frame 100 along the first direction; the extraction first mounting member 540 is slidably disposed on the extraction base 530 along the second direction; the extraction slide rail 550 is disposed on the extraction first mounting member 540 and extends along the third direction; the beating bracket 560 is connected to the beating driving member 511, and the beating bracket 560 is slidably disposed on the extraction slide rail 550 through the first slider, and the beating sleeve 512 is detachably connected to the beating bracket 560; the magnetic bracket 570 is connected to the extraction driving member 521, and the magnetic bracket 570 is slidably disposed on the extraction slide rail 550 through the second slider, and the magnetic rod 522 is disposed on the magnetic bracket 570. This arrangement enables the magnetic bar 522 and the flapping sleeve 512 to move synchronously, so as to transfer the magnetic beads to the cleaning station; that is, after the magnetic bar 522 is inserted into the flapping sleeve 512, it is simultaneously transferred by the first mounting member 540 and the extraction base 530, reducing the transfer components, making the structure more compact, and making the control logic simpler. Among them, the flapping sleeve 512 includes a flapping body 5121 and a flapping part 5122 arranged on the flapping body 5121, and the flapping part 5122 is provided with a receiving groove for inserting the magnetic bar 522, and both sides of the flapping body 5121 are each used as a plug-in part to be plugged and matched with a plug-in groove of the flapping bracket 560. Among them, the plug-in groove extends along the second direction, and one end is not closed to form an entrance. Among them, the wall thickness of the receiving groove inside the flapping part 5122 is the same everywhere, so that when the magnetic bar 522 is inserted, the adsorption force on the magnetic beads is the same everywhere.

[0127] In order to increase the intensity of sample mixing in the test tube, the beating portion 5122 needs to reciprocate along the third direction at a faster speed. However, the faster beating portion 5122 will generate a greater impact force with the sample, thereby causing sample splashing. To avoid this situation, in some embodiments, the lower end of the beating portion 5122 is tapered, so that the contact area when initially entering the sample is smaller. As the beating portion 5122 continues to descend, the contact area with the sample gradually increases, thereby reducing the possibility of sample splashing.

[0128] In some embodiments, the outer periphery of the beating portion 5122 is spiral-shaped, so that the beating portion 5122 can drive the sample to generate vortexes during the descending or ascending process, thereby further improving the mixing effect of the sample and the magnetic beads.

[0129] Furthermore, the lower end of the slapping portion 5122 is conical, and the outer periphery of the conical portion of the slapping portion 5122 has a spiral groove or a spiral protrusion.

[0130] Among them, the flapping driving member 511 and the flapping bracket 560 are connected by belt transmission; the extraction driving member 521 and the magnetic bracket 570 are connected by belt transmission. Specifically, the output end of the flapping driving member 511 is provided with a flapping driving wheel, the flapping driven wheel is rotatably arranged on the first extraction mounting member 540, the flapping belt is wound between the flapping driving wheel and the flapping driven wheel, and is connected to the flapping bracket 560. The output end of the extraction driving member 521 is provided with an extraction driving wheel, the extraction driven wheel is rotatably arranged on the first extraction mounting member 540, the extraction belt is wound between the extraction driving wheel and the extraction driven wheel, and is connected to the magnetic bracket 570. Among them, the first slider is located below the second slider to avoid interference during the movement process.

[0131] The molecular diagnostic pre-treatment device also includes a second consumable storage mechanism 730, which includes a second consumable base 731, a second consumable carrier 732, and a second consumable drive 733. The second consumable base 731 is provided on the rack 100; the second consumable carrier 732 is slidably provided on the second consumable base 731 along the second direction, and is used to hold consumables; the second consumable drive 733 is provided on the second consumable base 731, and is transmission-connected with the second consumable carrier 732, so as to drive the second consumable carrier 732 to move between the extraction station in the rack 100 and outside the rack 100. The consumables contained in the second consumable carrier 732 are test tubes, and when the magnetic beads in the test tubes are taken away, the second consumable storage mechanism 730 moves the test tubes out of the rack 100.

[0132] In order to facilitate the magnetic beads to adsorb nucleic acid molecules, the samples in the test tube need to be kept in a suitable temperature environment. For this purpose, the second consumable storage mechanism 730 also includes a heating element 734, which is used to heat the test tube on the second consumable carrier 732.

[0133] In this embodiment, it is necessary to transfer the magnetic beads with nucleic acid molecules to the cleaning tube of the cleaning station to clean the nucleic acid molecules from the magnetic beads. For this purpose, the molecular diagnosis pre-treatment device also includes a third consumable storage mechanism 740, and the third consumable storage mechanism 740 includes a third consumable base 741, a third consumable carrier 742 and a third consumable drive 743. Among them, the third consumable base 741 is arranged on the rack 100; the third consumable carrier 742 is slidably arranged on the third consumable base 741 along the second direction, and is used to hold consumables; the third consumable drive 743 is arranged on the third consumable base 741, and is transmission-connected with the third consumable carrier 742 to drive the third consumable carrier 742 to move between the cleaning station in the rack 100 and outside the rack 100. Among them, the consumables held in the third consumable carrier 742 are cleaning tubes, which are used to clean the nucleic acid molecules on the magnetic beads.

[0134] The third consumable material storage mechanism 740 further includes a refrigeration module 744 to lower the temperature of the cleaning tube so as to preserve the nucleic acid molecules in the cleaning tube at low temperature.

[0135] In some embodiments, the transmission mechanism 600 includes a transmission base 680 and a first transmission belt 681 and a second transmission belt 682 provided on the transmission base 680. The first transmission belt 681 is used to move the sample tube from the outside to the first loading position in the rack 100 along the first direction; the second transmission belt 682 is used to move the carrier with the test tube from the second loading position to the outside of the rack 100 along the first direction. The first transmission belt 681 and the second transmission belt 682 are docked in the left and right directions to complete the transfer of the carrier with the test tube. After the carrier with the test tube is transferred from the first transmission belt 681 to the second transmission belt 682, the second transmission belt 682 continues to move to transfer the carrier with the test tube to the second loading position, and the carrier with the test tube is transferred to the extraction position through the grabbing mechanism 770. The structure of the grabbing mechanism 770 can refer to the transmission clamp 670 and its driving mechanism. Of course, the grabbing mechanism 770 can adopt a mechanical arm.

[0136] Specifically, there are two first transmission belts 681, and the two first transmission belts 681 are arranged at intervals along the second direction to better carry the carrier with the sample tube. The transmission mechanism 600 further includes a stopper 683, wherein the stopper 683 is slidably arranged on the frame 100 along the third direction, and is provided with an elongated groove extending along the second direction, and the output end of the stopper driving member 684 is provided with a transmission cam, and the convex block of the transmission cam is eccentrically arranged and penetrates the elongated groove. During the rotation of the stopper driving member 684, the stopper 683 is driven to rise to intercept the carrier of the first loading station, or to descend to avoid the carrier.

[0137] To improve production needs, in some embodiments, the molecular diagnostic pretreatment device also includes an air filtering mechanism 750 and a shell 760. The shell 760 has a processing chamber inside, and each mechanism is located in the processing chamber. The air filtering mechanism 750 is used to filter the air in the processing chamber to improve the cleanliness of the processing chamber. The shell 760 can also separate the processing chamber from the outside world to prevent the sample from being contaminated.

[0138] In some embodiments, the rack 100 includes two sub-racks, each of which has a storage space, and the two storage spaces are connected by a connecting channel, wherein the cover opening mechanism and the cup separation mechanism 400 are located in one of the storage spaces, and the extraction mechanism 500 is located in the other storage space, and the cross-section of the connecting channel is smaller than the storage space. This arrangement allows the two sub-racks to be spliced, and when part of the structure needs to be adjusted to meet different application scenarios, it can be replaced separately; at the same time, refer to Figure 1 , Figure 2 and Fig.14When the cleaning tube containing the processed nucleic acid molecule solution is removed, just open a accommodating space on the right side, and the accommodating space on the left side will not be connected to the outside world on a large scale, which reduces the probability of contamination, avoids work suspension, and improves work efficiency.

[0139] Furthermore, the air filtering mechanism 750 is arranged on the sub-bracket with the cleaning station, that is, the air filtering mechanism 750 is arranged on the sub-bracket on the right side, wherein the first air inlet is arranged on the sub-bracket with the right side and connected to the accommodating space, and the second air inlet is arranged on the sub-bracket with the left side and connected to the accommodating space. When the cleaning pipe is removed, the air filtering mechanism 750 continues to work, so that the airflow in the accommodating space on the left side will flow into the accommodating space with the cleaning station through the connecting channel, thereby further reducing the possibility of the accommodating space with the cleaning station being contaminated.

[0140] This embodiment also provides a molecular diagnosis pre-processing method, which is applicable to the molecular diagnosis pre-processing device in any of the above embodiments, and includes the following steps:

[0141] The sample tube is transferred to the cover opening station of the rack 100; the sample tube is fixed by the tube fixing assembly 300, and the cover is clamped and rotated by the clamping assembly 200 to remove the cover from the sample tube.

[0142] The sample in the sample tube is divided into test tubes at a plurality of cupping stations by the cupping mechanism 400 , and the test tube is moved to the extraction station by the transmission mechanism 600 .

[0143] The sample in the test tube is beaten by the beating sleeve 512 of the beating assembly 510 to fully mix the magnetic beads with the sample.

[0144] The magnetic rod 522 is inserted into the flapping sleeve 512 in the test tube to adsorb the magnetic beads to the outer wall of the flapping sleeve 512, and then the flapping sleeve 512 together with the magnetic rod 522 are pulled out of the test tube.

[0145] The above method allows the molecular pretreatment process to be carried out in one device, avoiding contact with the outside world and being less susceptible to contamination; at the same time, it reduces the degree of manual intervention, improves work efficiency, and improves detection consistency; finally, by beating the sample with the beating sleeve 512, the mixing effect of the magnetic beads and the sample can be improved, and the nucleic acid molecule extraction effect can be improved; in addition, the magnetic rod 522 is inserted into the beating sleeve 512, and the magnetic beads are adsorbed on the outer wall of the beating sleeve 512, and when the beating sleeve 512 and the magnetic rod 522 are located in the cleaning tube, the magnetic rod 522 is pulled out, and the magnetic beads naturally fall into the cleaning tube.

[0146] In the cleaning tube, the beating sleeve 512 reciprocates to complete the mixing of the magnetic beads and the cleaning solution while cleaning the beating sleeve 512; at the same time, since the magnetic rod 522 is always out of contact with the magnetic beads, the cleaning of the magnetic rod 522 is avoided.

[0147] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. For those skilled in the art, various obvious changes, readjustments and substitutions can be made without departing from the protection scope of the present invention. It is not necessary and impossible to list all the embodiments here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A molecular diagnosis pre-processing device, characterized in that: include: Rack(100); A cover opening mechanism, the cover opening mechanism comprising a clamping assembly (200) for clamping the cover body and a tube fixing assembly (300) for fixing the sample tube, wherein the clamping end of the clamping assembly (200) can rotate relative to the tube fixing assembly (300) around the axis of the sample tube; A cup-splitting mechanism (400), the cup-splitting mechanism (400) comprising a cup-splitting moving assembly and a pipette (490) arranged at an output end of the cup-splitting moving assembly, the pipette (490) being used to transfer the sample in the sample tube into a plurality of test tubes; The extraction mechanism (500) comprises a flapping assembly (510) and an extraction assembly (520), wherein the flapping assembly (510) comprises a flapping driving member (511) and a flapping sleeve (512), wherein the flapping sleeve (512) is slidably arranged on the frame (100) along the axial direction of the test tube, and the flapping driving member (511) is arranged on the frame (100) and is transmission-connected with the flapping sleeve (512) to drive the flapping sleeve (512) to reciprocate in the test tube at the extraction station; the extraction assembly (520) comprises a flapping driving member (511) and a flapping sleeve (512), wherein the flapping driving member (511) is arranged on the frame (100) and is transmission-connected with the flapping sleeve (512) to drive the flapping sleeve (512) to reciprocate in the test tube at the extraction station; 20) comprises an extraction drive member (521) and a magnetic rod (522), wherein the magnetic rod (522) is slidably arranged on the frame (100) along the axial direction of the test tube, and the extraction drive member (521) is arranged on the frame (100) and is transmission-connected with the magnetic rod (522) to drive the magnetic rod (522) to enter the flapping sleeve (512) or move out of the flapping sleeve (512); when the magnetic rod (522) enters the flapping sleeve (512), the magnetic beads in the test tube are adsorbed on the outer wall of the flapping sleeve (512); The transmission mechanism (600) is used to move the sample tube from the outside to the cover opening station, and to move the test tube from the cup separation station to the extraction station, and finally move the test tube to the outside.

2. The molecular diagnosis pre-processing device according to claim 1, characterized in that: The extraction mechanism (500) comprises: An extraction base (530) is slidably disposed on the frame (100) along a first direction; The first extraction mounting member (540) is slidably disposed on the extraction base (530) along the second direction; An extraction slide rail (550) is provided on the extraction first mounting member (540) and extends along a third direction; A flapping bracket (560), the flapping bracket (560) is connected to the flapping driving member (511) and is slidably disposed on the extraction slide rail (550) via a first sliding block, and the flapping sleeve (512) is detachably connected to the flapping bracket (560); A magnetic bracket (570), the magnetic bracket (570) is connected to the extraction drive member (521) and is slidably disposed on the extraction slide rail (550) via a second slider, and the magnetic rod (522) is disposed on the magnetic bracket (570); and / or, The end of the flapping sleeve (512) is a conical structure; and / or, The end of the magnetic rod (522) is a conical structure.

3. The molecular diagnosis pre-processing device according to claim 1, characterized in that: The clamping assembly (200) comprises: A clamping base (210) is slidably disposed on the frame (100) along a first direction; A clamping first mounting member (220) is slidably disposed on the clamping base (210) along a second direction; A second clamping mounting member (230) is slidably disposed on the first clamping mounting member (220) along a third direction; A rotating driving member (240), the rotating driving member (240) being slidably disposed on the clamping second mounting member (230) along a third direction; Two elastic members, the two elastic members applying elastic forces in opposite directions to the rotary drive member (240) so as to make the rotary drive member (240) float on the clamping second mounting member (230); A first clamping driving member (250), wherein the first clamping driving member (250) is arranged at an output end of the rotating driving member (240); Two cover opening clamping claws (260) are respectively arranged at two output ends of the first clamping driving member (250) so as to be close to or away from each other.

4. The molecular diagnosis pre-processing device according to claim 1, characterized in that: The pipe fixing assembly (300) comprises: Tube fixing seat (310); A plurality of tube fixing members (320), wherein two adjacent tube fixing members (320) form a group and are arranged at intervals on the tube fixing seat (310) and can be moved closer to or farther from each other; A first screw rod (330) is rotatably mounted on the pipe fixing seat (310) and has at least a plurality of sets of thread segments, each set of thread segments including driving threads with opposite rotation directions; A plurality of connecting flanges (340), two adjacent connecting flanges (340) form a group, and are respectively connected to two pipe fixing members (320) in the same group, and are respectively matched with two driving threads in the same group of threaded segments; The second clamping driving member (350) is disposed on the pipe fixing seat (310) and is transmission-connected to the first screw rod (330).

5. The molecular diagnosis pre-processing device according to any one of claims 1 to 4, characterized in that: The cup-dispensing moving assembly comprises: A cup-dispensing base (410) is slidably disposed on the frame (100) along a first direction; A first cup-distributing mounting member (420) is slidably disposed on the cup-distributing base (410) along a second direction; A first cup-separating driving member (430) is arranged on the first cup-separating mounting member (420), and an output end thereof is drivingly connected to the cup-separating base (410); A second cup-separating mounting member (440) is slidably mounted on the first cup-separating mounting member (420) along a third direction, and the pipette (490) is mounted on the second cup-separating mounting member (440); A second cup-separating driving member (470) is arranged on the first cup-separating mounting member (420), and an output end is connected to the second cup-separating mounting member (440); and / or, The molecular diagnosis pre-treatment device further comprises a mixing mechanism (710), wherein the mixing mechanism (710) comprises: A mixing support (711), arranged on the frame (100); A mixing support (712) is slidably disposed on the mixing support (711) along a second direction; The mixing driving member (713) is disposed on the mixing support (711) and is drivingly connected to the mixing bearing member (712) to drive the mixing bearing member (712) to move back and forth.

6. The molecular diagnosis pre-processing device according to claim 5, characterized in that: The cup separation moving assembly further comprises a cup separation rack (480) arranged on the cup separation base (410) along the second direction, and a cup separation gear meshing with the cup separation rack (480) is installed at the output end of the cup separation first driving member (430).

7. The molecular diagnosis pre-processing device according to claim 5, characterized in that: The transmission mechanism (600) comprises: A transmission base (610) is slidably disposed on the cup-distributing base (410) along a second direction; A first transmission driving member (630) is disposed on the cup-distributing base (410), and an output end thereof is in transmission connection with the transmission base (610); A first transmission mounting member (620) is slidably disposed on the transmission seat (610) along a third direction; a second transmission driving member (650) is disposed on the transmission seat (610), and an output end is connected to the first transmission mounting member (620); The transmission clamp (670) is arranged on the transmission first mounting member (620) and moves synchronously therewith to grab and drive the sample tube from the first loading station in the frame (100) to the lid opening station.

8. The molecular diagnosis pre-treatment device according to any one of claims 1 to 4, characterized in that: The molecular diagnosis pre-treatment device further comprises a first consumable material storage mechanism (720), wherein the first consumable material storage mechanism (720) comprises: A first consumable base (721), arranged on the frame (100); A first consumable material carrier (722), slidably disposed on the first consumable material base (721) along a second direction, and used for containing consumable materials; The first consumable driving member (723) is arranged on the first consumable base (721) and is transmission-connected to the first consumable bearing member (722) so as to drive the first consumable bearing member (722) to move within the cup dispensing station in the frame (100) and outside the frame (100).

9. The molecular diagnosis pre-processing device according to any one of claims 1 to 4, characterized in that: The transmission mechanism (600) comprises: A first transmission belt (681) for moving the sample tube from the outside to a first loading position in the rack (100) along a first direction; The second transmission belt (682) is used to move the test tube from the first transmission belt (681) to the second loading station along the first direction and then move the test tube from the second loading station to the outside of the frame (100).

10. A molecular diagnosis pretreatment method, applicable to the molecular diagnosis pretreatment device according to any one of claims 1 to 9, characterized in that: The following steps are involved: The sample tube is transferred to the cover opening station of the rack (100); the sample tube is fixed by a tube fixing assembly (300), and the cover body is clamped and rotated by a clamping assembly (200) to remove the cover body from the sample tube; The sample in the sample tube is transferred to test tubes at a plurality of cupping stations by means of a cupping mechanism (400), and the test tube is moved to an extraction station by means of a transmission mechanism (600); The sample in the test tube is beaten by a beating sleeve (512) of a beating assembly (510) so as to fully mix the magnetic beads with the sample; The magnetic rod (522) is inserted into the flapping sleeve (512) in the test tube to adsorb the magnetic beads to the outer wall of the flapping sleeve (512), and then the flapping sleeve (512) together with the magnetic rod (522) is pulled out of the test tube.

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