A lysozyme extraction and separation device

Abstract

The application relates to the technical field of lysozyme separation and extraction, in particular to a lysozyme separation and extraction device, which comprises a centrifugal table and a separation cylinder, the upper end of the separation cylinder is provided with an upper cylinder cover and a lower cylinder cover, the upper cylinder cover is provided with an L-shaped stirring sheet, and the bottom of the separation cylinder is provided with a separation component, plant tissue fluid and buffer solution can be effectively mixed, the L-shaped stirring sheet can reciprocatingly lift and drop, the crushing effect of the tissue fragments in the mixture is greatly improved, the release of lysozyme from the tissue fragments is more sufficient, and more lysozyme can be collected, after the centrifugal work is completed, the separation component can be used to strip the precipitate into the lower cylinder cover, then the lower cylinder cover is rotated and fallen from the threaded butt joint, the precipitate can be quickly separated from the separation cylinder and collected, and the supernatant well placed above the separation cylinder is not affected.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of lysozyme separation and extraction, in particular to a lysozyme extraction and separation device. BACKGROUND

[0002] Lysozyme is an enzyme that exists widely in organisms and has important biological functions. It exists widely in various tissues and secretions of animals, and many plant tissues also contain lysozyme, such as in the organs of plant roots, stems, leaves, flowers, etc., which plays a role in the process of plant resisting pathogenic bacteria invasion.

[0003] When extracting lysozyme from plants, plant tissues rich in lysozyme are selected, such as the fruits of papaya and the leaves of spinach, which are washed, cut and then ground in a mortar to break the cells and obtain plant tissue fluid. The ground plant tissue fluid is filtered with gauze or filter paper to remove large tissue fragments, and then an appropriate amount of extraction buffer is added, and the lysozyme is released into the buffer. Then the plant tissue fluid and the buffer are centrifuged to obtain a crude extract of plant-derived lysozyme. However, the centrifuge currently used for separation of the crude extract has the problem that the plant tissue fluid and the buffer inside the reagent tube cannot be mixed at the beginning of starting the centrifuge, which affects the separation of lysozyme. In addition, after separating the supernatant and the precipitate, the precipitate is separated from the test tube, which can cause some of the precipitate to mix again into the supernatant. SUMMARY

[0004] The present application aims to provide a lysozyme extraction and separation device to solve the problems of the plant tissue fluid and the buffer inside the reagent tube being unable to mix and the precipitate being separated from the test tube, which can cause some of the precipitate to mix again into the supernatant.

[0005] To achieve the above-mentioned purpose, the present application provides the following technical solution: a lysozyme extraction and separation device, comprising a centrifuge table and a separation cylinder installed on the centrifuge table and used for centrifuging the extraction liquid, wherein the upper and lower end faces of the separation cylinder are open structures, and the upper and lower ends of the separation cylinder are respectively provided with an upper cylinder cover and a lower cylinder cover which are threadedly connected and used for sealing the open end faces;

[0006] A plurality of L-shaped stirring foils are installed on the upper cylinder cover in a ring-shaped distribution, which are used for stirring and breaking the extraction liquid. A separation component is installed at the bottom of the separation cylinder, which is used for abutting the lower cylinder cover and separating the supernatant from the precipitate;

[0007] The separating component comprises a sleeve pipe and an annular rubber ring, the sleeve pipe is sleeved on the cylinder body of the separating cylinder near the bottom and is elastically connected with the separating cylinder, the bottom of the sleeve pipe is fixedly installed with an inner support frame, a drum-shaped plugging column is installed on the inner support frame, the annular rubber ring is fixedly embedded on the inner cylinder wall of the separating cylinder and is located below the drum-shaped plugging column.

[0008] The lysozyme extraction and separation equipment further comprises a driving component, the separating cylinder and the L-shaped stirring sheet are driven to rotate through the driving component.

[0009] Preferably, the inner support frame is fixedly installed with a support rod coaxially arranged with the sleeve pipe, the drum-shaped plugging column is fixedly installed on the top of the support rod, and the drum-shaped plugging column is in interference fit with the annular rubber ring.

[0010] Preferably, a plurality of annularly distributed blanking cavities are formed in the inner support frame, and a threaded butt joint for threadedly connecting the lower cylinder cover is fixedly embedded in the bottom of the inner support frame.

[0011] Preferably, a rotating disc is rotatably installed in the middle of the upper cylinder cover, and a lifting and rotating component for driving the L-shaped stirring sheet to reciprocatingly lift and rotate at high speed in the separating cylinder is installed on the rotating disc.

[0012] The lifting and rotating component comprises a reciprocating screw rod, the reciprocating screw rod is rotatably installed on the rotating disc and is coaxially arranged with the rotating disc, the top of the reciprocating screw rod extends out of the rotating disc and is formed with a cross slot, a limiting rod is fixedly installed on the rotating disc and is parallelly arranged with the reciprocating screw rod, the top of the limiting rod extends out of the rotating disc and is fixedly embedded with a threaded column A, a threaded seat and a threaded sleeve which are threadedly connected and are distributed above and below are sleeved on the reciprocating screw rod, the threaded seat and the threaded sleeve are rotatably connected, the limiting rod passes through a through hole formed in the threaded seat and is slidably connected with the through hole, and the L-shaped stirring sheet is fixedly installed on the bottom of the threaded sleeve.

[0013] Preferably, the driving component comprises an outer gear ring and an inner gear ring, the outer gear ring and the inner gear ring are rotatably installed on the centrifugal table and are coaxially arranged with the plug-in through hole formed in the shaft center of the centrifugal table, the inner gear ring is located at the periphery of the outer gear ring, a middle gear wheel which is meshingly connected is installed between the outer gear ring and the inner gear ring, and a servo motor for driving the middle gear wheel to rotate is fixedly installed on the centrifugal table.

[0014] Preferably, a plurality of annularly distributed erection lugs are fixedly embedded on the cylinder body of the separating cylinder, a plurality of annularly distributed lug grooves which are matched with the erection lugs are formed in the outer gear ring, and a threaded hole is formed in each lug groove.

[0015] Preferably, a connecting lever component for connecting the reciprocating screw rod is installed on the inner gear ring.

[0016] The connecting rod component comprises a support column which is rotationally mounted on the inner ring gear, and a micro air cylinder is fixedly mounted on the support column, the output end of the micro air cylinder is vertically upward, and a horizontally arranged middle connecting plate is fixedly mounted thereon, and a cross-shaped insertion plate is fixedly mounted on the middle connecting plate, and the cross-shaped insertion plate is matched with a cross-shaped groove opened on the top of the reciprocating screw rod.

[0017] Preferably, a positioning rod is fixedly mounted on the centrifugal platform between the outer ring gear and the inner ring gear, and a threaded B column is fixedly mounted on the positioning rod, and a double-head sleeve plate is sleeved between the threaded B column and the threaded A column and is fixed by a fastening nut.

[0018] Preferably, the elastic member is provided with multiple groups and is annularly distributed on the top of the sleeve pipe, the elastic member comprises a sliding rod which is fixedly mounted on the top of the sleeve pipe and penetrates an outer ring along which is integrally formed on the outer cylinder wall of the separation cylinder, and the sliding rod is in sliding connection with the outer ring along, a spring in a compressed state is sleeved on the sliding rod, and the two ends of the spring are fixedly connected with the lower surface of the outer ring along and the upper surface of the sleeve pipe respectively, and a rod cap is fixedly mounted on the top of the sliding rod.

[0019] Preferably, an elastic corrugated sleeve coaxially arranged with the separation cylinder is fixedly embedded at the edge of the bottom of the separation cylinder and the inner support frame, and an arc-shaped outer protruding along is fixedly embedded on the cover opening of the lower cylinder cover.

[0020] Compared with the prior art, the beneficial effects of the present application are:

[0021] 1. The single motor working mode drives the separation cylinder and the L-shaped stirring sheet to start, so that the separation cylinder can effectively and fully mix the plant tissue fluid and the buffer solution before centrifugation. Since the L-shaped stirring sheet can reciprocally lift and lower, the crushing effect on the tissue fragments in the mixture is improved, so that the release of lysozyme from the tissue fragments is more sufficient, and more lysozyme can be collected.

[0022] 2. After the centrifugation is completed, the separation component can strip the precipitate into the lower cylinder cover, and then the lower cylinder cover is rotated off from the threaded butt joint, so that the precipitate can be quickly separated from the separation cylinder and collected, and the supernatant well placed above the separation cylinder is not affected. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 The structure schematic view of a preferred embodiment of the lysozyme extraction and separation equipment provided by the present application;

[0024] Figure 2 The structure schematic view of the separation cylinder and the upper cylinder cover and the lower cylinder cover after separation; Figure 1

[0025] ​Figure 3 Fig. 1 is a schematic diagram of the centrifugal table without the separation cylinder installed; Figure 2 Fig. 2 is a schematic diagram of the cross-sectional structure of the upper cylinder cover and the lower cylinder cover when installed on the separation cylinder;

[0026] Figure 4 Fig. 3 is a schematic diagram of the cross-sectional structure of the upper cylinder cover; Figure 3 Fig. 4 is a schematic diagram of the cross-sectional structure of the separation cylinder;

[0027] Figure 5 Fig. 5 is a schematic diagram of the cross-sectional structure of the lower cylinder cover; Figure 3 Fig. 6 is a schematic diagram of the cross-sectional structure of the centrifugal table without the separation cylinder installed;

[0028] Figure 6 Fig. 7 is a schematic diagram of the cross-sectional structure of the centrifugal table bottom without the separation cylinder installed; Figure 3 Fig. 8 is a schematic diagram of the cross-sectional structure of the connecting rod component;

[0029] Figure 7 Fig. 9 is a schematic diagram of the structure of the double-headed sleeve plate; Figure 1 Fig. 10 is a schematic diagram of the structure of the connecting rod component;

[0030] Figure 8 Fig. 11 is a schematic diagram of the structure of the double-headed sleeve plate; Figure 7 Fig. 12 is a schematic diagram of the structure of the double-headed sleeve plate;

[0031] Figure 9 Fig. 13 is a schematic diagram of the structure of the double-headed sleeve plate; Figure 7 Fig. 14 is a schematic diagram of the structure of the double-headed sleeve plate;

[0032] Figure 10 Fig. 15 is a schematic diagram of the structure of the double-headed sleeve plate; Figure 1 Fig. 16 is a schematic diagram of the structure of the double-headed sleeve plate;

[0033] Figure 11 Fig. 17 is a schematic diagram of the structure of the double-headed sleeve plate; Figure 4 Fig. 18 is a schematic diagram of the structure of the double-headed sleeve plate;

[0034] Fig. 1 is a schematic diagram of the centrifugal table without the separation cylinder installed; Fig. 2 is a schematic diagram of the cross-sectional structure of the upper cylinder cover and the lower cylinder cover when installed on the separation cylinder; Fig. 3 is a schematic diagram of the cross-sectional structure of the upper cylinder cover; Fig. 4 is a schematic diagram of the cross-sectional structure of the separation cylinder; Fig. 5 is a schematic diagram of the cross-sectional structure of the lower cylinder cover; Fig. 6 is a schematic diagram of the cross-sectional structure of the centrifugal table without the separation cylinder installed; Fig. 7 is a schematic diagram of the cross-sectional structure of the centrifugal table bottom without the separation cylinder installed; Fig. 8 is a schematic diagram of the cross-sectional structure of the connecting rod component; Fig. 9 is a schematic diagram of the structure of the double-headed sleeve plate; Fig. 10 is a schematic diagram of the structure of the connecting rod component; Fig. 11 is a schematic diagram of the structure of the double-headed sleeve plate; Fig. 12 is a schematic diagram of the structure of the double-headed sleeve plate; Fig. 13 is a schematic diagram of the structure of the double-headed sleeve plate; Fig. 14 is a schematic diagram of the structure of the double-headed sleeve plate; Fig. 15 is a schematic diagram of the structure of the double-headed sleeve plate; Fig. 16 is a schematic diagram of the structure of the double-headed sleeve plate; Fig. 17 is a schematic diagram of the structure of the double-headed sleeve plate; Fig. 18 is a schematic diagram of the structure of the double-headed sleeve plate; DETAILED DESCRIPTION

[0035] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0036] Please refer to Figures 1 to 11 The lysozyme extraction and separation equipment provided in the embodiments of the present application comprises a centrifugal table 1, a separation cylinder 2, an L-shaped stirring sheet 3 and a driving component 4.

[0037] In the embodiments of the present application, please refer to Figure 1 、 Figure 2 、 Figure 7 and Figure 8 The centrifugal table 1 is provided with the separation cylinder 2 for centrifugal treatment of the extraction liquid, the upper and lower end faces of the separation cylinder 2 are both open structures, and the upper and lower ends of the separation cylinder 2 are respectively provided with an upper cylinder cover 21 and a lower cylinder cover 22 which are threadedly connected and used for sealing the open end faces.

[0038] The lysozyme extraction and separation equipment further comprises the driving component 4, the driving component 4 comprises an outer gear ring 41 and an inner gear ring 42, the outer gear ring 41 and the inner gear ring 42 are both rotationally installed on the centrifugal table 1 and coaxially arranged with a pipe insertion through hole 1a formed at the shaft center of the centrifugal table 1, the inner gear ring 42 is located at the periphery of the outer gear ring 41, a middle gear 43 is installed between the outer gear ring 41 and the inner gear ring 42 in meshing connection, a servo motor 44 is fixedly installed on the centrifugal table 1 and used for driving the middle gear 43 to rotate, the pipe insertion through hole 1a is formed at the shaft center of the centrifugal table 1, a plurality of annularly distributed erection lugs 23 are fixedly embedded on the cylinder body of the separation cylinder 2, a plurality of lug grooves 41a which are annularly distributed and matched with the erection lugs 23 are formed on the outer gear ring 41, and a threaded hole 41b is formed on each lug groove 41a.

[0039] It should be noted that the lower cylinder cover 22 is threadedly connected with the threaded butt joint 242 on the separation cylinder 2 to block the opening at the bottom of the separation cylinder 2, then the extraction liquid is poured into the separation cylinder 2 through the opening at the top of the separation cylinder 2, the upper cylinder cover 21 is screwed, the separation cylinder 2 is inserted into the pipe insertion through hole 1a formed in the centrifugal table 1 until the erection lugs 23 fall into the lug grooves 41a, then the erection lugs 23 are fixed into the threaded holes 41b formed in the outer gear ring 41 by screws, so that the installation of the separation cylinder 2 on the outer gear ring 41 is completed, and the subsequent start of the servo motor 44 can drive the inner gear ring 42 to rotate through the middle gear 43, so that the separation cylinder 2 can rotate at high speed and drive the internal extraction liquid to separate into supernatant and precipitate.

[0040] In order to reduce the force of the separation cylinder 2 colliding with the hole wall of the insertion tube through hole 1a, a rubber ring is sleeved on the insertion tube through hole 1a, thereby effectively protecting the separation cylinder 2.

[0041] In order to better observe the separation effect of the extraction liquid in the separation cylinder 2, the separation cylinder 2 can be made of high-strength transparent glass.

[0042] In the embodiments of the present application, please refer to Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 7 、 Figure 9 、 Figure 10 and Figure 11 A plurality of annularly distributed L-shaped stirring foils 3 are installed on the upper cylinder cover 21, and the extraction liquid is stirred and broken by the L-shaped stirring foils 3. Specifically, a rotating disc 211 is installed in the middle of the upper cylinder cover 21 and is rotationally connected, and a lifting and rotating component 5 for driving the L-shaped stirring foils 3 to reciprocatingly lift and rotate at high speed in the separation cylinder 2 is installed on the rotating disc 211. The lifting and rotating component 5 includes a reciprocating screw rod 51 which is rotationally installed on the rotating disc 211 and coaxially arranged with the rotating disc 211, and the top of the reciprocating screw rod 51 extends out of the rotating disc 211 and is provided with a cross slot 51a. A limiting rod 52 which is parallel to the reciprocating screw rod 51 is also fixedly installed on the rotating disc 211, and the top of the limiting rod 52 extends out of the rotating disc 211 and is fixedly embedded with a threaded A column 521. A threaded seat 53 and a threaded sleeve 54 which are distributed in an up-down manner and are threadedly connected are sleeved on the reciprocating screw rod 51, and the threaded seat 53 and the threaded sleeve 54 are rotationally connected. The limiting rod 52 penetrates through a through hole of the threaded seat 53 and is slidingly connected with the through hole. The L-shaped stirring foils 3 are fixedly installed at the bottom of the threaded sleeve 54.

[0043] When the L-shaped stirring foils 3 are needed to be used to stir and break the extraction liquid, the driving component 4 drives the separation cylinder 2 and the L-shaped stirring foils 3 in opposite directions of rotation by additionally adding a connecting rod component 6 and a double-headed sleeve plate 72. Specifically, the connecting rod component 6 for connecting the reciprocating screw rod 51 is installed on the outer gear ring 41. The connecting rod component 6 includes a support column 61 which is rotationally installed on the inner gear ring 42, and a micro pneumatic cylinder 62 is fixedly installed on the support column 61. The output end of the micro pneumatic cylinder 62 is vertically upward and is fixedly installed with a horizontally arranged middle connecting plate 63, and the middle connecting plate 63 is fixedly installed with a cross-shaped plug plate 64. The cross-shaped plug plate 64 is matched with the cross slot 51a at the top of the reciprocating screw rod 51. The centrifugal table 1 between the outer gear ring 41 and the inner gear ring 42 is fixedly installed with a positioning rod 7, and the positioning rod 7 is fixedly installed with a threaded B column 71. The threaded B column 71 and the threaded A column 521 are sleeved with the double-headed sleeve plate 72 which is fixed by a fastening nut 73.

[0044] It needs to be explained: before starting the servo motor 44, the double-head sleeve plate 72 is respectively sleeved on the threaded B column 71 and the threaded A column 521, and then the fastening nut 73 is respectively installed on the threaded B column 71 and the threaded A column 521 for fixation, so that the double-head sleeve plate 72 cooperates with the positioning rod 7 to fix the rotating disc 211. Since the rotating disc 211 is rotationally connected with the upper barrel cover 21, when the separation barrel 2 rotates and centrifugates, the rotating disc 211 and the limiting rod 52 on the rotating disc 211 are always in a locked state. After the double-head sleeve plate 72 is installed, the rotating support column 61 and the reciprocating lead screw 51 make the cross-shaped insert plate 64 on the middle connecting plate 63 correspond to the cross-shaped groove 51a on the reciprocating lead screw 51. Then the output end of the micro pneumatic cylinder 62 is opened, so that the cross-shaped insert plate 64 is inserted into the cross-shaped groove 51a. Therefore, the rotation of the inner gear ring 42 can drive the reciprocating lead screw 51 to rotate through the connecting lever component 6.

[0045] Since the middle connecting gear 43 is located between the outer gear ring 41 and the inner gear ring 42 and is meshingly connected with the outer gear ring 41 and the inner gear ring 42 respectively, when the servo motor 44 is started, the separation barrel 2 and the reciprocating lead screw 51 rotate in opposite directions. Since the threaded seat 53 and the threaded sleeve 54 are rotationally connected and are both threadedly connected with the reciprocating lead screw 51, and the threaded seat 53 is limited by the limiting rod 52, the threaded seat 53 can ascend and descend along the reciprocating lead screw 51. Under the action of the downward thrust or upward pulling force of the threaded seat 53, the threaded sleeve 54 is driven to reciprocally slide and rotate along the reciprocating lead screw 51. Therefore, the L-shaped stirring sheet 3 at the bottom of the threaded sleeve 54 can stir and crush the extraction liquid in the separation barrel 2.

[0046] The rotation of the L-shaped stirring sheet 3 can enhance the mixing of the plant tissue liquid and the buffer liquid. Since the L-shaped stirring sheet 3 on the threaded sleeve 54 can reciprocally ascend and descend, the stirring center can be constantly changed, further realizing the mixing degree of the plant tissue liquid and the buffer liquid. The L-shaped stirring sheet 3 can also secondarily crush the tissue fragments in the plant tissue liquid, so that the lysozyme is more fully released.

[0047] It also needs to be explained that after stirring for a period of time, the micro pneumatic cylinder 62 is opened to make the cross-shaped insert plate 64 slide out of the cross-shaped groove 51a on the reciprocating lead screw 51, so that the reciprocating lead screw 51 loses the rotation driving force, and the reciprocating lead screw 51 and the L-shaped stirring sheet 3 gradually stop. Therefore, the stirring effect of the L-shaped stirring sheet 3 is cancelled, and the separation barrel 2 continues to rotate at high speed, so that the stirring of the mixed liquid by the L-shaped stirring sheet 3 does not cause the mixed liquid to be difficult to effectively separate into supernatant and precipitate.

[0048] The single motor working mode drives the separation cylinder 2 and the L-shaped stirring sheet 3 to start, so that the plant tissue fluid and the buffer can be effectively mixed before centrifugation by the separation cylinder 2. The L-shaped stirring sheet 3 can reciprocatingly lift and drop, so that the crushing effect of the mixture on the tissue fragments is greatly improved, the tissue fragments release lysozyme more fully, and more lysozyme is collected. After stirring and crushing, the L-shaped stirring sheet 3 can stop stirring and crushing without stopping the machine, and the separation cylinder 2 can still work normally.

[0049] The horizontal part of the L-shaped stirring sheet 3 is provided with blade surfaces on both sides, so that the tissue fragments can be better crushed. The vertical part of the L-shaped stirring sheet 3 is higher than the length of the limiting rod 52, so that the rotation of the horizontal part of the L-shaped stirring sheet 3 is not hindered by the limiting rod 52.

[0050] It is worth noting that before the separation cylinder 2 is installed on the outer ring gear 41, the support column 61 is rotated to make the center line of the cross-shaped insert plate 64 on the center plate 63 deviate from the axis of the outer ring gear 41. After the separation cylinder 2 is installed, the support column 61 is rotated again to make the center line of the cross-shaped insert plate 64 on the center plate 63 coincide with the axis of the outer ring gear 41. The support column 61 is arranged on the inner ring gear 42, so that the center plate 63 does not hinder the installation and disassembly of the separation cylinder 2.

[0051] In the present application, in order to avoid the mutual interference between the double-head sleeve plate 72 and the center plate 63, the double-head sleeve plate 72 is installed at a height lower than that of the center plate 63.

[0052] In the embodiments of the present application, please refer to Figure 1 , Figure 2 , Figure 3 , Figure 5 , Figure 6 The bottom of the separation cylinder 2 is provided with a separation component 24 for abutting the lower cylinder cover 22 and separating the supernatant from the sediment. The separation component 24 comprises a sleeve pipe 241 and an annular rubber ring 246. The sleeve pipe 241 is sleeved on the cylinder body of the separation cylinder 2 close to the bottom and is elastically connected with the separation cylinder 2. Specifically, an elastic member 8 is arranged between the separation cylinder 2 and the sleeve pipe 241. The elastic member 8 comprises a plurality of groups and is annularly distributed on the top of the sleeve pipe 241. The elastic member 8 comprises a sliding rod 81. The sliding rod 81 is fixedly installed on the top of the sleeve pipe 241 and penetrates an outer ring 25 integrally formed on the outer cylinder wall of the separation cylinder 2 and is in sliding connection with the outer ring 25. A spring 82 in a compressed state is sleeved on the sliding rod 81. The two ends of the spring 82 are fixedly connected with the lower surface of the outer ring 25 and the upper surface of the sleeve pipe 241, respectively. A rod cap is fixedly installed on the top of the sliding rod 81.

[0053] And the bottom of the sleeve pipe 241 is fixedly installed with an inner support frame 243, the inner support frame 243 is installed with a drum-shaped plugging column 245, an annular rubber ring 246 is fixedly embedded on the inner cylinder wall of the separation cylinder 2 and located below the drum-shaped plugging column 245, specifically, the inner support frame 243 is fixedly installed with a support rod 244 coaxially arranged with the sleeve pipe 241, the drum-shaped plugging column 245 is fixedly installed on the top of the support rod 244, and the drum-shaped plugging column 245 is in interference fit with the annular rubber ring 246, and a plurality of annularly distributed material dropping cavities 243a are arranged on the inner support frame 243, and the bottom of the inner support frame 243 is fixedly embedded with a threaded butt joint 242 for threadedly connecting the lower cylinder cover 22.

[0054] It should be noted that: due to the sleeve pipe 241 under the action of the spring 82, the drum-shaped plugging column 245 in the separation component 24 is embedded into the annular rubber ring 246 and forms a sealing structure, thereby blocking the bottom of the separation cylinder 2, after the centrifugal work is completed, the separation cylinder 2 is taken off from the centrifugal table 1, at this time, the precipitate is located on the conical column above the drum-shaped plugging column 245, the sleeve pipe 241 is pushed up to make the sleeve pipe 241 slide up along the separation cylinder 2 (in this process, the spring 82 is further compressed), so that the drum-shaped plugging column 245 slides out of the annular rubber ring 246 and opens the formed sealing structure, so that the precipitate on the conical column above the drum-shaped plugging column 245 can slide down along the conical column above the drum-shaped plugging column 245 and fall into the lower cylinder cover 22 through the material dropping cavities 243a arranged on the inner support frame 243;

[0055] And after the collection of the precipitate is completed, the sleeve pipe 241 is loosened, so that the sleeve pipe 241 drives the drum-shaped plugging column 245 to be embedded into the annular rubber ring 246 and forms a sealing structure under the action of the spring 82, then the lower cylinder cover 22 is rotated and fallen from the threaded butt joint 242, so that the precipitate can be quickly separated from the separation cylinder 2 and collected, and the supernatant liquid standing above the separation cylinder 2 is not affected.

[0056] It is worth noting that: the top of the annular rubber ring 246 is provided with an outwardly expanding opening, which is matched with the conical column below the drum-shaped plugging column 245, thereby improving the sealing performance of the sealing structure formed by the drum-shaped plugging column 245 and the annular rubber ring 246.

[0057] Among them, the bottom of the separation cylinder 2 and the edge of the inner support frame 243 are fixedly embedded with an elastic corrugated sleeve 9 coaxially arranged with the separation cylinder 2, to prevent solution from leaking from the bottom of the separation cylinder 2 and the edge of the inner support frame 243, and the cover of the lower cylinder cover 22 is fixedly embedded with a ring of arc-shaped outer protruding edges 221, to facilitate the discharge of the precipitate.

[0058] The above merely illustrates the embodiments of the present application, and is not intended to limit the patent scope of the present application. Any equivalent structure or equivalent process conversion, or direct or indirect application in other related technical fields, which is made according to the content of the present application, shall be included in the patent protection scope of the present application.

Claims

1. A lysozyme extraction and separation device, comprising: a centrifugal table (1), and a separation cylinder (2) mounted on the centrifugal table (1) and used for centrifugal treatment of an extraction liquid by the centrifugal table (1), and the upper and lower end faces of the separation cylinder (2) are both open structures, and the upper and lower ends of the separation cylinder (2) are respectively provided with an upper cylinder cover (21) and a lower cylinder cover (22) which are threadedly connected and used for sealing the open end faces, characterized in that: a plurality of L-shaped stirring foils (3) are arranged in a ring shape on the upper cylinder cover (21) and used for stirring and crushing the extraction liquid, wherein a rotating disc (211) is mounted in the middle of the upper cylinder cover (21) and connected in rotation, and a lifting and rotating component (5) for driving the L-shaped stirring foils (3) to reciprocatingly lift and rotate at high speed in the separation cylinder (2) is mounted on the rotating disc (211), and the lifting and rotating component (5) comprises a reciprocating screw rod (51) which is rotatably mounted on the rotating disc (211) and coaxially arranged with the rotating disc (211), and the top of the reciprocating screw rod (51) extends out of the rotating disc (211) and is provided with a cross slot (51a), and a limiting rod (52) which is arranged in parallel with the reciprocating screw rod (51) is fixedly mounted on the rotating disc (211), and the top of the limiting rod (52) extends out of the rotating disc (211) and is fixedly embedded with a threaded A column (521); a separation component (24) for abutting the lower cylinder cover (22) and separating supernatant from precipitate is mounted at the bottom of the separation cylinder (2), the separation component (24) comprises a sleeve pipe (241) and an annular rubber ring (246), the sleeve pipe (241) is sleeved on the cylinder body of the separation cylinder (2) near the bottom and elastically connected with the separation cylinder (2), and an inner support frame (243) is fixedly mounted at the bottom of the sleeve pipe (241), a drum-shaped plugging column (245) is mounted on the inner support frame (243), and the annular rubber ring (246) is fixedly embedded on the inner cylinder wall of the separation cylinder (2) and located below the drum-shaped plugging column (245); the lysozyme extraction and separation device further comprises a driving component (4) for driving the separation cylinder (2) and the L-shaped stirring foils (3) to rotate, and the driving component (4) comprises an outer gear ring (41) and an inner gear ring (42), the outer gear ring (41) and the inner gear ring (42) are both rotatably mounted on the centrifugal table (1) and coaxially arranged with a pipe through hole (1a) provided at the shaft center of the centrifugal table (1), the inner gear ring (42) is located at the periphery of the outer gear ring (41), a middle gear (43) is mounted between the outer gear ring (41) and the inner gear ring (42) and engagedly connected, and a servo motor (44) for driving the middle gear (43) to rotate is fixedly mounted on the centrifugal table (1); a connecting rod component (6) for connecting the reciprocating screw rod (51) is mounted on the inner gear ring (42). The centrifugal table (1) is fixedly installed with a positioning rod (7) between the outer gear ring (41) and the inner gear ring (42), and the positioning rod (7) is fixedly installed with a threaded B column (71), and the threaded B column (71) is sleeved with a double-head sleeve plate (72) fixed by a fastening nut (73).

2. The lysozyme extraction and isolation apparatus of claim 1, wherein: The inner support frame (243) is fixedly installed with a support rod (244) coaxially arranged with the sleeve pipe (241), the drum-shaped plugging column (245) is fixedly installed on the top of the support rod (244), and the drum-shaped plugging column (245) is in interference fit with the annular rubber ring (246).

3. The lysozyme extraction and isolation apparatus of claim 2, wherein: A plurality of annularly distributed blanking cavities (243a) are formed in the inner support frame (243), and the bottom of the inner support frame (243) is fixedly embedded with a threaded butt joint (242) for threadedly connecting the lower cylinder cover (22).

4. The lysozyme extraction and isolation apparatus of claim 1, wherein: The reciprocating lead screw (51) is sleeved with an upper and lower distributed threaded seat (53) and a threaded sleeve (54) connected by threads, and the threaded seat (53) and the threaded sleeve (54) are rotationally connected, the limiting rod (52) passes through the through hole formed in the threaded seat (53) and is slidingly connected with the through hole, and the L-shaped stirring sheet (3) is fixedly installed on the bottom of the threaded sleeve (54).

5. The lysozyme extraction and isolation apparatus of claim 4, wherein: A plurality of annularly distributed erection lugs (23) are fixedly embedded on the cylinder body of the separation cylinder (2), a plurality of annularly distributed lug grooves (41a) matched with the erection lugs (23) are formed in the outer gear ring (41), and a threaded hole (41b) is formed in each lug groove (41a).

6. The lysozyme extraction and isolation apparatus of claim 4, wherein: The connecting rod component (6) comprises a support column (61) rotationally installed on the inner gear ring (42), and a micro air cylinder (62) fixedly installed on the support column (61), the output end of the micro air cylinder (62) is vertically upward and fixedly installed with a horizontally arranged middle connecting plate (63), and the middle connecting plate (63) is fixedly installed with a cross-shaped plug-in plate (64), the cross-shaped plug-in plate (64) is matched with a cross-shaped groove (51a) formed on the top of the reciprocating lead screw (51).

7. The lysozyme extraction and isolation apparatus of claim 1, wherein: An elastic member (8) is arranged between the separation cylinder (2) and the sleeve pipe (241), the elastic member (8) is arranged in multiple groups and annularly distributed on the top of the sleeve pipe (241), the elastic member (8) comprises a sliding rod (81) fixedly installed on the top of the sleeve pipe (241) and penetrating an outer ring (25) integrally formed on the outer cylinder wall of the separation cylinder (2), and the sliding rod (81) is slidingly connected with the outer ring (25), a spring (82) in a compressed state is sleeved on the sliding rod (81), and the two ends of the spring (82) are fixedly connected with the lower surface of the outer ring (25) and the upper surface of the sleeve pipe (241) respectively, and a rod cap is fixedly installed on the top of the sliding rod (81).

8. The lysozyme extraction and isolation apparatus of claim 1, wherein: The bottom of the separation cylinder (2) and the edge of the inner support frame (243) are fixedly embedded with an elastic corrugated sleeve (9) coaxially arranged with the separation cylinder (2), and the cover opening of the lower cylinder cover (22) is fixedly embedded with an arc-shaped outer protruding edge (221).

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