Reagent collection monitoring method, device, equipment, storage medium and program product

Abstract

The invention discloses a reagent collection monitoring method, device and equipment, a storage medium and a program product, and relates to the technical field of sample analys.The reagent collection monitoring method comprises the steps that after collection operation starts to be executed, a target collection pressure value of a pressure sensor arranged between a pipetting needle and a quantitative power device is determined, when the pressure sensor collects a reagent or gas, pressure values collected by the pressure sensor are different, and the pipetting needle is a reagent needle or a sampling needle; judging whether the target acquisition pressure value is in a corresponding preset acquisition pressure range when an acquisition object is a reagent or not; if the target acquisition pressure value is not within the preset acquisition pressure range, abnormal information is fed back to display equipment, so that quantitative and accurate reagent acquisition is ensured. The problem that accurate and quantitative suction of the latex reagent cannot be stably achieved in a simple mode is solved, and accurate and quantitative suction of the latex reagent is achieved.

Description

Technical Field

[0001] The present application relates to the technical field of sample analysis, and in particular to a reagent collection monitoring method, device, equipment, storage medium and program product. Background Art

[0002] At present, for the detection process of specific proteins in the blood, it is usually necessary to control the absorption process of latex reagents to prevent the problem of insufficient latex reagents in the latex reagent tank, resulting in insufficient absorption of latex reagents and inaccurate quantification of latex reagents, so as to ensure the correctness of the results of specific protein detection using latex reagents.

[0003] There are currently two methods for aspirating latex reagents. One is to use a reagent needle with a complex structure to detect the liquid level of the latex reagent to ensure that the reagent needle aspiration hole is immersed below the reagent liquid level; the other is to fill a certain amount of latex reagent, record the number of aspirations, and replace the latex reagent after the number of aspirations reaches a certain number. The above methods require the use of a reagent needle with a complex structure for liquid level detection or quantitative and accurate latex reagent filling, which makes it impossible to stably achieve accurate and quantitative aspiration of the latex reagent in a simple way. Summary of the invention

[0004] The main purpose of the present application is to provide a reagent collection monitoring method, device, equipment, storage medium and program product, aiming to solve the technical problem that accurate quantitative absorption of latex reagents cannot be stably achieved in a simple manner.

[0005] To achieve the above objectives, the present application proposes a reagent collection monitoring method, the method comprising:

[0006] After the collection operation is started, a target collection pressure value of a pressure sensor disposed between the pipette needle and the quantitative power device is determined, wherein the pressure value collected by the pressure sensor is different when the collection object is a reagent or a gas, and the pipette needle is a reagent needle or a sampling needle;

[0007] Determining whether the target collection pressure value is within a preset collection pressure range corresponding to when the collection object is a reagent;

[0008] If the target collection pressure value is not within the preset collection pressure range, the abnormal information is fed back to the display device to ensure accurate quantitative collection of the reagent.

[0009] In one embodiment, the step of determining whether the target collection pressure value is within a preset collection pressure range corresponding to when the collection object is a reagent comprises:

[0010] Get the current collection time point;

[0011] Obtaining a target acquisition pressure range corresponding to the current acquisition time point in the preset acquisition pressure range;

[0012] It is determined whether the target acquisition pressure value is within the target acquisition pressure range.

[0013] In one embodiment, before the step of determining the target collection pressure value of the pressure sensor disposed between the pipette needle and the quantitative power device after the collection operation is started, the method further includes:

[0014] Obtaining a preset collection flow rate, a preset collection volume, and a preset pressure fluctuation range;

[0015] Determine a preset collection pressure curve corresponding to the preset collection flow rate and the preset collection volume, wherein the abscissa of the preset collection pressure curve is the collection time, and the ordinate is the collection pressure value;

[0016] Taking the collected pressure value at each time point in the preset collected pressure curve as the reference collected pressure value;

[0017] The preset pressure fluctuation range is superimposed on the reference acquisition pressure value to obtain the preset acquisition pressure range.

[0018] In one embodiment, after the step of superimposing the preset pressure fluctuation range on the basis of the reference collected pressure value to obtain the preset collected pressure range, the step further includes:

[0019] When receiving a collection instruction, moving the pipette needle to a preset collection point corresponding to the collection instruction, wherein the collection instruction is a sample collection instruction or a reagent collection instruction;

[0020] When the pipetting needle reaches the preset collection point, a collection operation is performed based on the preset collection flow rate and the preset collection volume, wherein the collection operation is a reagent collection operation or a sample collection operation.

[0021] In one embodiment, the preset collection points include a preset first collection point for obtaining a sample and a preset second collection point for obtaining a reagent, and when the pipette needle reaches the preset collection point, based on the preset collection flow rate and the preset collection volume, the step of performing a collection operation includes:

[0022] If the preset collection point is the preset first collection point, performing a sample collection operation based on the preset collection flow rate and the preset collection volume;

[0023] If the preset collection point is the preset second collection point, air is sucked based on a preset air suction amount;

[0024] Based on the preset collection flow rate and the preset collection volume, a reagent collection operation is performed.

[0025] In one embodiment, after the step of feeding back abnormal information to a display device if the target acquisition pressure value is not within the preset acquisition pressure range, the method further includes:

[0026] When receiving the instruction to end the collection operation, cleaning the pipetting needle;

[0027] Moving the pipette needle to a preset dispensing point corresponding to the preset collection point to perform an analysis liquid dispensing operation, wherein the analysis liquid includes a reagent and a sample;

[0028] When the instruction for dispensing the analysis liquid is received, the pipetting needle is cleaned.

[0029] In addition, to achieve the above purpose, the present application also proposes a reagent collection monitoring device, the reagent collection monitoring device comprising:

[0030] A state detection module, used for determining a target collection pressure value of a pressure sensor disposed between a pipetting needle and a quantitative power device after the collection operation is started, wherein the pressure value collected by the pressure sensor is different when the collection object is a reagent or a gas, and the pipetting needle is a reagent needle or a sampling needle;

[0031] A pressure comparison module, used to determine whether the target collection pressure value is within a preset collection pressure range corresponding to when the collection object is a reagent;

[0032] The information feedback module is used to feed back abnormal information to the display device if the target collection pressure value is not within the preset collection pressure range, so as to ensure the accurate quantitative collection of the reagent.

[0033] In addition, to achieve the above-mentioned purpose, the present application also proposes a reagent collection monitoring device, which includes: a memory, a processor, and a computer program stored in the memory and executable on the processor, and the computer program is configured to implement the steps of the reagent collection monitoring method described above.

[0034] In addition, to achieve the above-mentioned purpose, the present application also proposes a storage medium, which is a computer-readable storage medium, and a computer program is stored on the storage medium. When the computer program is executed by a processor, the steps of the reagent collection monitoring method described above are implemented.

[0035] In addition, to achieve the above-mentioned purpose, the present application also provides a computer program product, which includes a computer program, and when the computer program is executed by a processor, the steps of the reagent collection monitoring method described above are implemented.

[0036] One or more technical solutions proposed in this application have at least the following technical effects:

[0037] After the collection operation is started, the present application determines the target collection pressure value of the pressure sensor arranged between the pipette needle and the quantitative power device, wherein the pressure sensor collects different pressure values ​​when the collection object is a reagent or a gas, and the pipette needle is a reagent needle or a sampling needle; it is judged whether the target collection pressure value is within the preset collection pressure range corresponding to the collection object being a reagent; if the target collection pressure value is not within the preset collection pressure range, the abnormal information is fed back to the display device to ensure the accuracy of the reagent collection quantification.

[0038] In the process of reagent collection, there may be insufficient reagents in the reagent compartment, resulting in inaccurate reagent collection quantification; when the reagent is insufficient, there will be a situation of gas absorption during the collection process, so that the collection pressure value collected by the pressure sensor is different from the collection pressure value during normal collection. Therefore, by setting a pressure sensor between the pipetting needle and the quantitative power device, the collection pressure value during the collection process can be obtained, and it can be determined whether the collection pressure value is within the preset collection pressure range. If the collection pressure value is not within the preset collection pressure range, it can be determined that there is an abnormality in the collection process, and the abnormal information is fed back to the display device for the user to process this abnormal collection, so as to ensure that the reagent collection is accurate. And because the collection process can be carried out using a reagent needle or a sampling needle, there is no need for a sampling needle with a complex structure, and there is no need for quantitative and accurate reagent filling. Therefore, the present application can stably achieve accurate quantitative absorption of latex reagents in a simple way. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0040] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0041] Figure 1 A schematic diagram of a process flow provided for Example 1 of the reagent collection monitoring method of the present application;

[0042] Figure 2 A schematic diagram of a first scenario provided in Example 1 of the reagent collection monitoring method of the present application;

[0043] Figure 3A schematic diagram of a second scenario provided in Example 1 of the reagent collection monitoring method of the present application;

[0044] Figure 4 A schematic diagram of a third scenario provided in Example 1 of the reagent collection monitoring method of the present application;

[0045] Figure 5 A schematic diagram of a fourth scenario provided in Example 1 of the reagent collection monitoring method of the present application;

[0046] Figure 6 A schematic diagram of the process provided for Example 2 of the reagent collection monitoring method of this application;

[0047] Figure 7 A schematic diagram of the process provided for Example 3 of the reagent collection monitoring method of this application;

[0048] Figure 8 A schematic diagram of the first scenario provided in Example 3 of the reagent collection monitoring method of the present application;

[0049] Fig. 9 A schematic diagram of a second scenario provided in Example 3 of the reagent collection monitoring method of the present application;

[0050] Fig.10 This is a schematic diagram of the module structure of the reagent collection monitoring device of the embodiment of the present application;

[0051] Fig.11 Schematic diagram of the equipment structure of the hardware operating environment involved in the reagent collection monitoring method in the embodiment of the present application.

[0052] The purpose, features and advantages of this application will be further described in conjunction with the embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0053] It should be understood that the specific embodiments described herein are only used to explain the technical solutions of the present application and are not used to limit the present application.

[0054] In order to better understand the technical solution of the present application, a detailed description will be given below in conjunction with the accompanying drawings and specific implementation methods.

[0055] It should be noted that the execution subject of this embodiment can be a computing service device with data processing, network communication and program running functions, such as a tablet computer, a personal computer, a mobile phone, etc., or an electronic device capable of realizing the above functions, a reagent collection monitoring device, etc. The following takes the reagent collection monitoring device as an example to illustrate this embodiment and the following embodiments.

[0056] Based on this, the present application embodiment provides a reagent collection monitoring method, referring to Figure 1 , Figure 1This is a flow chart of the first embodiment of the reagent collection monitoring method of the present application.

[0057] In this embodiment, the reagent collection monitoring method includes steps S10 to S30:

[0058] Step S10, after the collection operation is started, determining the target collection pressure value of the pressure sensor disposed between the pipette needle and the quantitative power device, wherein the pressure value collected by the pressure sensor is different when the collection object is a reagent or a gas, and the pipette needle is a reagent needle or a sampling needle;

[0059] It should be noted that with the development of medical device technology, the promotion of clinical needs and clinical applications, more and more test parameters are output in the field of blood testing. From the earliest two-group, three-group, and five-category to the joint test of blood routine parameters and specific protein parameters. Human blood contains a variety of different proteins, which are derived from tissue cells and are functional proteins of blood, also known as specific proteins. Many diseases can cause changes in the concentration of these proteins. Therefore, changes in the concentration of these proteins can characterize the patient's physical health status, such as C-reaction protein (CRP), serum amyloid A (SAA), procalcitonin (PTC), etc. Among them, changes in the concentration of C-reaction protein can reflect changes in inflammation in the body of the person being measured.

[0060] Therefore, it is often necessary to detect the concentration of these proteins and judge the patient's physical health status based on the concentration of these proteins. The detection principle of specific proteins often uses immunoturbidimetry, which is divided into transmission method and scattering method. Immunoturbidimetry requires the use of latex reagent (also known as R2). Latex reagent is used in small quantities, is expensive, and needs to be refrigerated at an appropriate temperature. Therefore, the instrument often uses a needle and a quantitative power device. The needle can be a separate reagent needle or a shared sample needle to achieve the absorption and distribution of latex reagents. The quantitative accuracy of latex reagents directly affects the measurement results, and then affects clinical diagnosis. Accurate quantification is based on the normal absorption of latex reagents. Therefore, it is necessary to ensure the normal absorption of latex reagents.

[0061] One of the current methods is to perform liquid level detection by using a reagent needle with a liquid level detection function. Specifically, by monitoring the difference in physical quantities when the reagent needle contacts the reagent and when it does not contact the reagent, it is ensured that the liquid aspiration hole of the reagent needle is immersed below the reagent liquid level, and then the quantitative power device is driven to absorb the reagent. The reagent needle of the liquid level detection method has a complex structure and high cost. It is difficult to achieve stability and reliability, and the reagent needle with a liquid level detection function often cannot be used as a sampling needle.

[0062] Another current method is to use ordinary reagent needles. Medical device manufacturers or reagent suppliers fill a certain amount of reagents when filling reagents. Each time the reagent needle is inserted into the bottom of the reagent bottle, the quantitative power device is driven to absorb the reagent. The software records the number of measurements after the reagent is replaced. When the number of measurements reaches the number of times the filling volume and the filling remainder are booked, it reports that there is no reagent and reminds the operator to replace the reagent. This method will cause waste of reagents, and due to the differences in quantitative power devices, if the quantitative filling system of the medical device manufacturer or reagent supplier is in the lower deviation, and the quantitative power device of a certain instrument is in the upper deviation, there may be a risk of running out of reagents without reporting a prompt. If the client operator pours the reagent from the old bottle into the new bottle for the sake of saving, the amount of reagent in the new bottle will increase. When the instrument reports a prompt, there is still a lot of reagent in the new reagent bottle, affecting the customer experience.

[0063] It can be understood that the collection process is the process of obtaining a small amount of reagents or samples for specific purposes from a larger batch of reagents or samples, including the operation of selecting and obtaining reagents or samples. However, the current reagent collection method has the problems of high difficulty in implementation and insufficient stability. This embodiment is to achieve accurate quantitative absorption of latex reagents in a stable manner in a simple manner. Therefore, this embodiment uses a common sampling needle to collect reagents and samples, and monitors the collection process, and does not rely on accurate quantitative filling of reagents.

[0064] Abnormal latex reagent absorption usually occurs when there is insufficient latex reagent in the reagent chamber, resulting in empty absorption when collecting the latex reagent. Therefore, to ensure normal absorption of the latex reagent, it is necessary to detect whether there is still latex reagent in the reagent chamber.

[0065] It should also be noted that the execution subject of this embodiment is a sample analyzer. Figure 2 , including a status check module, the status check module is used to detect and monitor various physical quantities of the analyzer (such as temperature, pressure and other physical quantities). The physical quantity concerned in this embodiment is pressure. Therefore, the status check module monitors and collects data from the pressure sensor in real time. The pressure sensor is used to detect the collected pressure value during the collection process.

[0066] It is understandable that the quantitative abnormality of the reagent is usually caused by insufficient reagent in the reagent chamber during the reagent suction process, resulting in empty suction, and empty suction will absorb some air, making the collection pressure values ​​during empty suction and normal suction different. And because the collection process is the process of moving the reagent or sample from the reagent chamber or sampling position to the quantitative power device pipeline, the pressure value during the collection process is related to the flow rate of the reagent or sample. Therefore, this embodiment obtains the target collection pressure value when the reagent collection operation is performed by designing a pressure sensor between the reagent needle and the quantitative power device pipeline.

[0067] The specific connection method of the pressure sensor is that the pressure sensor is connected between the quantitative power device and the needle through a pipeline system composed of a hose, a joint, etc., refer to Figure 3 The needle is driven by an actuator module to move the needle along a predetermined trajectory. The quantitative power device can be a syringe, a plunger pump, or other components that can achieve quantitative reagents. This embodiment uses a syringe for description. Common syringes are as follows: Figure 3 As shown in . Syringes often use stepper motors through a combination of screws and nuts to achieve the up and down movement of the syringe plunger. When liquid needs to be sucked in, the plunger moves downward, and the inner cavity of the syringe body that cooperates with the plunger becomes larger, generating a certain negative pressure, thereby sucking the liquid into the needle and the pipeline system; when the liquid needs to be discharged, the plunger moves upward to discharge the liquid in the pipeline system and the needle into the target container (such as a reaction pool).

[0068] Step S20, determining whether the target collection pressure value is within a preset collection pressure range corresponding to when the collection object is a reagent;

[0069] It should be noted that the sample analyzer also includes a control module, which is used to control the entire sample analysis process, and the status check module transmits the collected pressure data to the control module. The control module also includes a memory and a comparator, the memory is used to store the normal pressure value during the collection process, and the comparator is used to compare the collected pressure value with the data inside the memory.

[0070] It is understandable that since the pressure value during the collection process is related to the flow rate of the reagent or sample, the collection pressure value during the normal collection process is different for different flow rates. And there will be some fluctuations in the pressure value during the normal collection process. Therefore, the target collection pressure value obtained does not have to be equal to the normal pressure value, but only needs to be within a certain range of the normal pressure value. Therefore, it is necessary to determine whether the target collection pressure value is within the preset collection pressure range.

[0071] In a feasible implementation manner, the specific implementation manner of the step of determining whether the target collection pressure value is within the preset collection pressure range corresponding to when the collection object is a reagent may also be:

[0072] The current acquisition time point is obtained, a target acquisition pressure range corresponding to the current acquisition time point in the preset acquisition pressure range is obtained, and it is determined whether the target acquisition pressure value is within the target acquisition pressure range.

[0073] It should be noted that the current collection time point is one of multiple time points after the reagent collection operation is started.

[0074] It is understandable that the reagent collection process is a continuous process, in which the normal collection pressure values ​​at different time points are different. Therefore, if you want to determine whether the target collection pressure value is within the preset collection pressure range, you need to compare it with the preset collection pressure range corresponding to the current time point. That is, you need to first obtain the current collection time point, then obtain the target collection pressure range corresponding to the current collection time point in the preset collection pressure range, and determine whether the target collection pressure value is within the target preset collection pressure range.

[0075] Step S30: If the target collection pressure value is not within the preset collection pressure range, the abnormal information is fed back to the display device to ensure accurate quantitative collection of the reagent.

[0076] It should be noted that the display device is a device such as a display screen that can display the information fed back by the analyzer. The comparator will compare the target acquisition pressure value with the preset acquisition pressure range, and determine whether the target acquisition pressure value is within the preset acquisition pressure range. The result of the judgment will be fed back to the control module for the control module to perform the next control operation.

[0077] It can be understood that if the target acquisition pressure value is within the preset acquisition pressure range, it indicates that the acquisition process is normal. If the target acquisition pressure value is not within the preset acquisition pressure range, it indicates that an acquisition abnormality occurs.

[0078] Specifically, Figure 4 To collect the pressure curve under normal conditions, Figure 5 This is a pressure curve diagram under abnormal collection conditions. It can be seen that the first part of the collection process is normal, but at a certain point in time during the process, the amount of reagent in the reagent chamber is insufficient, resulting in air suction during the collection process, and air or a mixture of air and liquid is sucked into the sampling needle, making the pressure value collected by the pressure sensor during the collection process different from the pressure value during the normal collection process. It can be seen that the earlier the abnormality occurs during the collection process, Figure 5 The red circle moves forward.

[0079] It should also be noted that the result of the abnormal acquisition will be fed back to the control module by the comparator. After the acquisition operation is completed, the control module will respond according to the specific situation.

[0080] It is understandable that collection abnormalities will lead to inaccurate quantification of latex reagents, thereby affecting the measurement results. Therefore, if the target collection pressure value is not within the preset collection pressure range, abnormal information needs to be fed back to ensure accurate reagent collection quantification.

[0081] The control module will stop the relevant actuators or block the test results of the sample to prevent the clinician's diagnosis from being affected by the wrong test parameter results. Since the abnormality of the collection process is usually caused by insufficient samples in the reagent compartment, the control module will also prompt the operator with the "no reagent" message through a display device (such as a display screen), prompting the operator to replace the reagent.

[0082] In summary, in this embodiment, after the collection operation is started, the target collection pressure value of the pressure sensor arranged between the pipette needle and the quantitative power device is determined, wherein the pressure value collected by the pressure sensor is different when the collection object is a reagent or a gas, and the pipette needle is a reagent needle or a sampling needle; it is judged whether the target collection pressure value is within the preset collection pressure range corresponding to the collection object being a reagent; if the target collection pressure value is not within the preset collection pressure range, the abnormal information is fed back to the display device to ensure the accuracy of the reagent collection quantification.

[0083] In the process of reagent collection, there may be insufficient reagent in the reagent chamber, resulting in inaccurate reagent collection quantity; when the reagent is insufficient, there will be gas absorption during the collection process, so that the collection pressure value collected by the pressure sensor is different from the collection pressure value during normal collection. Therefore, by setting a pressure sensor between the pipette needle and the quantitative power device, the collection pressure value during the collection process can be obtained, and it can be determined whether the collection pressure value is within the preset collection pressure range. If the collection pressure value is not within the preset collection pressure range, it can be determined that there is an abnormality in the collection process, and the abnormal information is fed back to the display device for the user to process the abnormal collection and ensure the accurate quantitative collection of the reagent. And because the collection process can be carried out using a reagent needle or a sampling needle, there is no need for a sampling needle with a complex structure, and there is no need for quantitative and accurate reagent filling. Therefore, this embodiment can stably achieve accurate quantitative absorption of latex reagents in a simple way.

[0084] Based on the first embodiment of the present application, in the second embodiment of the present application, the same or similar contents as those in the above-mentioned embodiment 1 can refer to the above introduction, and will not be repeated later. Figure 6 Before step S10, the reagent collection monitoring method further includes steps S01 to S03:

[0085] Step S01, obtaining a preset collection flow rate, a preset collection volume and a preset pressure fluctuation range;

[0086] It should be noted that the preset collection flow rate, the preset collection volume and the preset pressure fluctuation range are set in the sample analyzer before starting the collection operation. Under normal circumstances, the preset collection flow rate, the preset collection volume and the preset pressure fluctuation range are fixed quantities, but when there is a need to change them, the above parameters can also be reset.

[0087] It is understandable that the collection flow rate during the collection process will affect the collection pressure value collected by the pressure sensor. The total duration of a collection operation can be determined by the preset collection volume and the preset collection flow rate. Therefore, the preset collection flow rate and the preset collection volume will affect the pressure curve during the normal collection process.

[0088] In the normal collection process, the collection pressure value will fluctuate. The preset fluctuation range can be used to determine the normal collection pressure value within the preset fluctuation range and the abnormal collection pressure value outside the preset fluctuation range during the collection and monitoring process.

[0089] Therefore, in order to determine the preset collection pressure range during the normal collection process, it is necessary to first obtain the preset collection flow rate, the preset collection volume and the preset pressure fluctuation range.

[0090] Furthermore, the viscosity of the reagent will also affect the collection pressure value during the collection process, and the viscosity of the reagent will be affected by temperature. Therefore, this embodiment can also use the relationship between the viscosity of the reagent and the temperature for pressure monitoring by configuring an ambient temperature sensor.

[0091] Moreover, for the quantitative power device pipeline, the pipeline length and thickness will also affect the collected pressure value during the collection process. Therefore, when there is a need for change, this embodiment can configure quantitative device pipelines of different lengths and thicknesses for pressure monitoring.

[0092] Step S02, determining a preset collection pressure curve corresponding to the preset collection flow rate and the preset collection volume, wherein the abscissa of the preset collection pressure curve is the collection time, and the ordinate is the collection pressure value;

[0093] It can be understood that by determining the preset collection flow rate and the preset collection volume, the reagent flow rate and the duration of a single collection during the collection process can be determined, and by monitoring the collection pressure during the normal collection process, the collection pressure curve from the beginning of the normal collection operation to the end of the collection operation can be obtained. Therefore, the collection pressure value at each time point of the collection pressure curve can be used as the collection pressure value at each time point during the normal collection process to determine the preset collection pressure range.

[0094] Therefore, in order to determine the preset collection pressure range at each time point during the normal collection process, it is necessary to determine the preset collection pressure curve corresponding to the preset collection flow rate and the preset collection volume.

[0095] Furthermore, when there is a need for modification, the present embodiment can also configure quantitative device pipelines of different lengths and thicknesses, and use the relationship between reagent viscosity and temperature for pressure monitoring.

[0096] Step S03, taking the collected pressure value at each time point in the preset collected pressure curve as a reference collected pressure value;

[0097] It is understandable that the preset collection pressure curve is obtained based on the collection pressure value and collection time collected by the pressure sensor during a normal reagent collection process. Therefore, the collection pressure value at each time point in the preset collection pressure curve is used as the reference collection pressure value to compare the pressure values ​​of the subsequent collection process.

[0098] Step S04, on the basis of the reference acquisition pressure value, superimposing the preset pressure fluctuation range to obtain the preset acquisition pressure range.

[0099] It can be understood that the preset pressure fluctuation range is equivalent to the collection pressure error range during the collection process. Therefore, by taking the collection pressure value at each time point in the preset collection pressure curve as the reference collection pressure value and superimposing the preset pressure fluctuation range, the preset collection pressure range at each time point can be obtained, thereby obtaining the preset collection pressure range.

[0100] Furthermore, when there is a need for change, the preset pressure fluctuation range can be changed. When a smaller preset pressure fluctuation range is set, the collection pressure fluctuation allowed during the collection process will also become smaller. Therefore, by setting the preset pressure fluctuation range, the collection pressure fluctuation allowed during the reagent collection monitoring process can be controlled.

[0101] In summary, the present embodiment first obtains a preset collection flow rate, a preset collection volume, and a preset pressure fluctuation range, and determines a preset collection pressure curve corresponding to the preset collection flow rate and the preset collection volume, wherein the horizontal coordinate of the preset collection pressure curve is the collection time, and the vertical coordinate is the collection pressure value, and the collection pressure value at each time point in the preset collection pressure curve is used as a reference collection pressure value, and on the basis of the reference collection pressure value, the preset pressure fluctuation range is superimposed to obtain the preset collection pressure range.

[0102] In order to detect abnormal reagent collection, it is necessary to compare the collection pressure value during the normal collection process with the collection pressure value obtained during the collection process. The collection pressure value during the normal collection process will be affected by the preset collection flow rate and the preset collection volume. By obtaining the preset collection flow rate and the preset collection volume, the collection pressure value at each time point in the normal collection process and the time of a single collection can be determined; and since the collection pressure value will fluctuate during the normal collection process, the collection pressure value at each time point in the normal collection process is used as a benchmark, and the preset pressure fluctuation range is added thereto, so that the preset collection pressure range at each collection time point in the normal collection process can be obtained to compare the collection pressure values.

[0103] Based on the first and second embodiments of the present application, in the third embodiment of the present application, the same or similar contents as those of the first and second embodiments can be referred to the above description, and will not be described in detail later. Figure 7 After step S04, the reagent collection monitoring method further includes steps A01 to A02:

[0104] Step A01, when a collection instruction is received, the pipette needle is moved to a preset collection point corresponding to the collection instruction, wherein the collection instruction is a sample collection instruction or a reagent collection instruction;

[0105] It should be noted that the sample analyzer also includes a routine blood test module, a specific protein test module and a reagent supply module, and the control module of the sample analyzer also includes a driver. The driver is used to drive the reagent supply module to provide corresponding reagents for the corresponding reaction pool, and drive the routine blood test module and the specific protein test module to complete corresponding actions and related parameter detection according to the preset program. The routine blood test module is used to perform routine blood tests on samples in the routine blood reaction pool to obtain at least one routine blood parameter; the specific protein detection module is used to detect samples in the corresponding specific protein reactor to obtain specific protein parameters; the reagent supply module is used to provide corresponding reagents for the corresponding reaction pool.

[0106] It should also be noted that, in the present embodiment, the pipette needle is a sampling needle, and the movement of the sampling needle includes horizontal movement and vertical movement. The movement of the sampling needle is performed through the collection component of the analyzer, and the sampling needle reaches the preset collection point corresponding to the collection instruction, including: the sampling needle reaches above the collection point corresponding to the collection instruction, and moves to the preset position in the corresponding reagent compartment or sampling position.

[0107] It can be understood that the collection process includes the collection of reagents and samples. Reagents refer to substances used in chemical analysis, experiments or diagnostic tests, and are the objects of analysis, from which information is extracted to understand the overall situation or make a diagnosis. Reagents and samples are in the corresponding reagent compartment and sampling position respectively. Therefore, when a collection instruction is received, it is necessary to determine the category of the collection instruction to perform different operations.

[0108] Since the reagents and samples are respectively in the corresponding reagent chamber and sampling position, and the reagents or samples in the reagent chamber or sampling position will have corresponding liquid level heights, when the sampling needle moves to above the reagent chamber or sampling position, the reagent cannot be collected. It is then necessary to move vertically to the preset collection point inside the reagent chamber or sampling position before the subsequent collection operation can be performed. Therefore, based on the judgment result, it is necessary to move the sampling needle to the preset collection point corresponding to the collection instruction.

[0109] Step A02, when the pipetting needle reaches the preset collection point, a collection operation is performed based on the preset collection flow rate and the preset collection volume, wherein the collection operation is a reagent collection operation or a sample collection operation.

[0110] It should be noted that the collection operation is completed through the cooperation of the sampling needle and the collection quantitative power device. The collection quantitative power device for the reagent and sample can be a separate quantitative device, such as different syringes, or can be shared, such as collecting through the same syringe.

[0111] It is understandable that before the collection operation is performed, the sampling needle has been moved to the preset collection point, and the preset collection flow rate and the preset collection volume have been set. Therefore, the collection operation can be performed based on the preset collection flow rate and the preset collection volume.

[0112] In a feasible implementation manner, the preset collection points include a preset first collection point for obtaining a sample and a preset second collection point for obtaining a reagent. When the pipette needle reaches the preset collection point, based on the preset collection flow rate and the preset collection volume, a specific implementation manner of performing the collection operation may also be:

[0113] If the preset collection point is the preset first collection point, a sample collection operation is performed based on the preset collection flow rate and the preset collection volume; if the preset collection point is the preset second collection point, air is aspirated based on the preset air aspiration volume, and a reagent collection operation is performed based on the preset collection flow rate and the preset collection volume.

[0114] It should be noted that the preset first collection point is the collection point corresponding to the sampling position, and the preset second collection point is the collection point corresponding to the reagent compartment.

[0115] It is understandable that if the preset collection point is the preset first collection point, it means that the sample needs to be collected, and the control module controls the quantitative power device to perform the collection operation. If the preset collection point is the preset second collection point, it means that the reagent needs to be collected, because the sample is usually collected before the reagent is collected, and the collection of the sample will absorb a certain amount of diluent in the sampling needle. Therefore, if the preset collection point is the preset second collection point, it is necessary to absorb air based on the preset air absorption amount to isolate the reagent to be absorbed and the diluent in the sampling needle, and then perform the collection operation based on the preset collection flow rate and the preset collection amount.

[0116] In a feasible implementation manner, after the step of feeding back abnormal information to a display device if the target acquisition pressure value is not within the preset acquisition pressure range, the following may also be performed:

[0117] When the instruction for ending the collection operation is received, the pipette needle is cleaned and moved to the preset dispensing point corresponding to the preset collection point to perform an analysis liquid dispensing operation, wherein the analysis liquid includes reagents and samples. When the instruction for ending the analysis liquid dispensing is received, the pipette needle is cleaned.

[0118] It should be noted that the routine blood test module includes a routine blood test device and a routine blood test pool for preparing the assigned sample, and the specific protein test device and the specific protein test pool for preparing the assigned sample. In this embodiment, since the pipette needle is a sampling needle, the sampling position, the reagent compartment, the routine blood test pool and the specific protein test pool are arranged in a straight line on the horizontal movement route of the collection component. Figure 8 If a separate reagent needle is used for reagent collection, the specific protein reaction pool is still arranged on the movement path of the collection component, but the reagent needle can use other layout methods.

[0119] It should also be noted that after the reagent or sample is absorbed, the outer wall of the sampling needle needs to be cleaned by a sampling needle cleaning device (such as a component for cleaning a swab). After the reagent or sample is dispensed, the inner and outer walls of the sampling needle need to be cleaned by a sampling needle cleaning device. The overall process is as follows. Fig. 9 .

[0120] It is understandable that when the collection operation end instruction is received, it means that the sampling needle has completed the collection of the reagent or sample. After the collection is completed, there will be adhered reagents or samples on the outer wall of the sampling needle. Therefore, the outer wall of the sampling needle needs to be cleaned to prevent affecting subsequent measurements.

[0121] When the instruction to end the analysis liquid distribution is received, it means that the sampling needle has completed the distribution of the reagent or sample. After the distribution is completed, there will be adhered reagents or samples on the inner and outer walls of the sampling needle. Therefore, the inner and outer walls of the sampling needle need to be cleaned to prevent affecting subsequent measurements.

[0122] In summary, when a collection instruction is received in this embodiment, the pipette needle is moved to a preset collection point corresponding to the collection instruction, wherein the collection instruction is a sample collection instruction or a reagent collection instruction. When the pipette needle reaches the preset collection point, a collection operation is performed based on the preset collection flow rate and the preset collection volume, wherein the collection operation is a reagent collection operation or a sample collection operation.

[0123] Since the collection process includes the collection of reagents and the collection of samples, the reagents and samples are respectively in the corresponding reagent compartment and sampling position, and the collection operation needs to be performed after the sampling needle reaches the preset collection point in the reagent compartment or sampling position. Because there will be a certain amount of diluent in the sampling needle before the reagent is collected, it is necessary to absorb air based on the preset air absorption amount to isolate the reagent to be absorbed and the diluent in the sampling needle. And since after the collection of the reagent or sample is completed, there will be adhered reagents or samples on the outer wall of the sampling needle, and the outer wall of the sampling needle needs to be cleaned, and after the distribution of the reagent or sample is completed, there will be adhered reagents or samples on the inner and outer walls of the sampling needle, and the inner and outer walls of the sampling needle need to be cleaned to prevent the subsequent measurement from being affected. This embodiment performs different operations based on different collection instructions, and cleans the sampling needle after the collection and distribution of the reagent and sample to prevent the subsequent measurement from being affected. This embodiment uses a shared sampling needle to complete the collection and distribution of reagents and samples, thereby realizing reagent collection monitoring in a simple way.

[0124] It should be noted that the above examples are only used to understand the present application and do not constitute a limitation on the reagent collection and monitoring method of the present application. More simple transformations based on this technical concept are all within the scope of protection of the present application.

[0125] This application also provides a reagent collection monitoring device, please refer to Fig.10 , the reagent collection monitoring device comprises:

[0126] The state detection module 10 is used to determine the target collection pressure value of the pressure sensor disposed between the pipette needle and the quantitative power device after the collection operation is started, wherein the pressure value collected by the pressure sensor is different when the collection object is a reagent or a gas, and the pipette needle is a reagent needle or a sampling needle;

[0127] The pressure comparison module 20 is used to determine whether the target collection pressure value is within a preset collection pressure range corresponding to when the collection object is a reagent;

[0128] The information feedback module 30 is used to feed back abnormal information to the display device if the target collection pressure value is not within the preset collection pressure range, so as to ensure accurate quantitative collection of reagents.

[0129] The reagent collection monitoring device provided by the present application adopts the reagent collection monitoring method in the above embodiment, which can solve the technical problem that the accurate quantitative absorption of latex reagents cannot be stably achieved in a simple manner. Compared with the related art, the beneficial effects of the reagent collection monitoring device provided by the present application are the same as the beneficial effects of the reagent collection monitoring method provided by the above embodiment, and the other technical features of the reagent collection monitoring device are the same as the features disclosed in the above embodiment method, which will not be repeated here.

[0130] The present application provides a reagent collection monitoring device, which includes: at least one processor; and a memory communicatively connected to the at least one processor; wherein the memory stores instructions executable by the at least one processor, and the instructions are executed by the at least one processor so that the at least one processor can execute the reagent collection monitoring method in the above-mentioned embodiment one.

[0131] Reference below Fig.11 , which shows a schematic diagram of the structure of a reagent collection monitoring device suitable for implementing an embodiment of the present application. The reagent collection monitoring device in the embodiment of the present application may include, but is not limited to, mobile terminals such as mobile phones, tablet computers, laptop computers, digital broadcast receivers, PDAs (Personal Digital Assistants), PMPs (Portable Media Players), vehicle-mounted terminals (such as vehicle-mounted navigation terminals), etc., and fixed terminals such as digital TVs, desktop computers, etc. Figure 5 The reagent collection monitoring device shown is merely an example and should not bring any limitation to the functions and scope of use of the embodiments of the present application.

[0132] like Fig.11As shown, the reagent acquisition monitoring device may include a processing device 1001 (e.g., a central processing unit, a graphics processing unit, etc.), which can perform various appropriate actions and processes according to a program stored in a read-only memory (ROM: Read Only Memory) 1002 or a program loaded from a storage device 1003 to a random access memory (RAM: Random Access Memory) 1004. In RAM1004, various programs and data required for the operation of the reagent acquisition monitoring device are also stored. The processing device 1001, ROM1002, and RAM1004 are connected to each other via a bus 1005. An input / output (I / O) interface 1006 is also connected to the bus. Typically, the following systems can be connected to the I / O interface 1006: an input device 1007 including, for example, a touch screen, a touch pad, a keyboard, a mouse, an image sensor, a microphone, an accelerometer, a gyroscope, etc.; an output device 1008 including, for example, a liquid crystal display (LCD: Liquid Crystal Display), a speaker, a vibrator, etc.; a storage device 1003 including, for example, a magnetic tape, a hard disk, etc.; and a communication device 1009. Communication device 1009 can allow reagent acquisition monitoring equipment to carry out wireless or wired communication with other equipment to exchange data. Although the reagent acquisition monitoring equipment with various systems is shown in the figure, it should be understood that it is not required to implement or have all the systems shown. More or less systems can be implemented or possessed alternatively.

[0133] In particular, according to the embodiments disclosed in the present application, the process described above with reference to the flowchart can be implemented as a computer software program. For example, the embodiments disclosed in the present application include a computer program product, which includes a computer program carried on a computer-readable medium, and the computer program includes a program code for executing the method shown in the flowchart. In such an embodiment, the computer program can be downloaded and installed from a network through a communication device, or installed from a storage device 1003, or installed from a ROM 1002. When the computer program is executed by the processing device 1001, the above-mentioned functions defined in the method of the embodiment disclosed in the present application are executed.

[0134] The reagent collection monitoring device provided by the present application adopts the reagent collection monitoring method in the above embodiment, which can solve the technical problem that the accurate quantitative absorption of latex reagents cannot be stably achieved in a simple manner. Compared with the related art, the beneficial effects of the reagent collection monitoring device provided by the present application are the same as the beneficial effects of the reagent collection monitoring method provided by the above embodiment, and the other technical features in the reagent collection monitoring device are the same as the features disclosed in the method of the previous embodiment, which will not be repeated here.

[0135] It should be understood that the various parts disclosed in this application can be implemented by hardware, software, firmware or a combination thereof. In the description of the above embodiments, specific features, structures, materials or characteristics can be combined in any one or more embodiments or examples in a suitable manner.

[0136] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art who is familiar with the present technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

[0137] The present application provides a computer-readable storage medium having computer-readable program instructions (ie, computer programs) stored thereon, and the computer-readable program instructions are used to execute the reagent collection monitoring method in the above-mentioned embodiment.

[0138] The computer-readable storage medium provided in the present application may be, for example, a USB flash drive, but is not limited to electrical, magnetic, optical, electromagnetic, infrared, or semiconductor systems, systems or devices, or any combination of the above. More specific examples of computer-readable storage media may include, but are not limited to: an electrical connection with one or more wires, a portable computer disk, a hard disk, a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disk read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the above. In this embodiment, the computer-readable storage medium may be any tangible medium containing or storing a program that can be used by or in combination with an instruction execution system, system or device. The program code contained on the computer-readable storage medium may be transmitted using any appropriate medium, including but not limited to: wires, optical cables, RF (Radio Frequency), etc., or any suitable combination of the above.

[0139] The computer-readable storage medium may be included in the reagent collection monitoring device; or may exist independently without being assembled into the reagent collection monitoring device.

[0140] The computer-readable storage medium carries one or more programs. When the one or more programs are executed by the reagent collection monitoring device, the reagent collection monitoring device is enabled to: execute the reagent collection monitoring method.

[0141] Computer program code for performing the operations of the present application may be written in one or more programming languages ​​or a combination thereof, including object-oriented programming languages ​​such as Java, Smalltalk, C++, and conventional procedural programming languages ​​such as "C" or similar programming languages. The program code may be executed entirely on the user's computer, partially on the user's computer, as a separate software package, partially on the user's computer and partially on a remote computer, or entirely on a remote computer or server. In the case of a remote computer, the remote computer may be connected to the user's computer through any type of network, including a local area network (LAN) or a wide area network (WAN), or may be connected to an external computer (e.g., via the Internet using an Internet service provider).

[0142] The flow chart and block diagram in the accompanying drawings illustrate the possible architecture, function and operation of the system, method and computer program product according to each embodiment of the present application. In this regard, each square box in the flow chart or block diagram can represent a module, a program segment or a part of a code, and the module, the program segment or a part of the code contains one or more executable instructions for realizing the specified logical function. It should also be noted that in some implementations as replacements, the functions marked in the square box can also occur in a sequence different from that marked in the accompanying drawings. For example, two square boxes represented in succession can actually be executed substantially in parallel, and they can sometimes be executed in the opposite order, depending on the functions involved. It should also be noted that each square box in the block diagram and / or flow chart, and the combination of the square boxes in the block diagram and / or flow chart can be implemented with a dedicated hardware-based system that performs a specified function or operation, or can be implemented with a combination of dedicated hardware and computer instructions.

[0143] The modules involved in the embodiments described in this application may be implemented by software or hardware, wherein the name of the module does not constitute a limitation on the unit itself in some cases.

[0144] The readable storage medium provided by the present application is a computer-readable storage medium, which stores computer-readable program instructions (i.e., computer programs) for executing the above-mentioned reagent collection monitoring method, and can solve the technical problem that the accurate quantitative absorption of latex reagents cannot be stably achieved in a simple manner. Compared with the prior art, the beneficial effects of the computer-readable storage medium provided by the present application are the same as the beneficial effects of the reagent collection monitoring method provided by the above-mentioned embodiment, and will not be repeated here.

[0145] The present application also provides a computer program product, including a computer program, which implements the steps of the above-mentioned reagent collection monitoring method when executed by a processor.

[0146] The computer program product provided by the present application can solve the technical problem that the accurate quantitative absorption of latex reagents cannot be stably achieved in a simple manner. Compared with the prior art, the beneficial effects of the computer program product provided by the present application are the same as the beneficial effects of the reagent collection monitoring method provided by the above embodiment, which will not be repeated here.

[0147] The above descriptions are only some embodiments of the present application, and are not intended to limit the patent scope of the present application. All equivalent structural changes made using the contents of the present application specification and drawings under the technical concept of the present application, or direct / indirect applications in other related technical fields are included in the patent protection scope of the present application.

Claims

1. A reagent collection monitoring method, characterized in that: The method includes: After the collection operation is started, a target collection pressure value of a pressure sensor disposed between the pipette needle and the quantitative power device is determined, wherein the pressure value collected by the pressure sensor is different when the collection object is a reagent or a gas, and the pipette needle is a reagent needle or a sampling needle; Determining whether the target collection pressure value is within a preset collection pressure range corresponding to when the collection object is a reagent; If the target collection pressure value is not within the preset collection pressure range, the abnormal information is fed back to the display device to ensure accurate quantitative collection of the reagent.

2. The method according to claim 1, characterized in that The step of determining whether the target collection pressure value is within a preset collection pressure range corresponding to when the collection object is a reagent comprises: Get the current collection time point; Obtaining a target acquisition pressure range corresponding to the current acquisition time point in the preset acquisition pressure range; It is determined whether the target acquisition pressure value is within the target acquisition pressure range.

3. The method according to claim 1, characterized in that Before the step of determining the target collection pressure value of the pressure sensor disposed between the pipetting needle and the quantitative power device after the collection operation is started, the method further includes: Obtaining a preset collection flow rate, a preset collection volume, and a preset pressure fluctuation range; Determine a preset collection pressure curve corresponding to the preset collection flow rate and the preset collection volume, wherein the abscissa of the preset collection pressure curve is the collection time, and the ordinate is the collection pressure value; Taking the collected pressure value at each time point in the preset collected pressure curve as the reference collected pressure value; The preset pressure fluctuation range is superimposed on the reference acquisition pressure value to obtain the preset acquisition pressure range.

4. The method according to claim 3, characterized in that After the step of obtaining the preset acquisition pressure range by superimposing the preset pressure fluctuation range on the basis of the reference acquisition pressure value, the method further includes: When receiving a collection instruction, moving the pipette needle to a preset collection point corresponding to the collection instruction, wherein the collection instruction is a sample collection instruction or a reagent collection instruction; When the pipetting needle reaches the preset collection point, a collection operation is performed based on the preset collection flow rate and the preset collection volume, wherein the collection operation is a reagent collection operation or a sample collection operation.

5. The method according to claim 4, characterized in that: The preset collection points include a preset first collection point for obtaining a sample and a preset second collection point for obtaining a reagent. When the pipette needle reaches the preset collection point, based on the preset collection flow rate and the preset collection volume, the steps of performing a collection operation include: If the preset collection point is the preset first collection point, performing a sample collection operation based on the preset collection flow rate and the preset collection volume; If the preset collection point is the preset second collection point, air is sucked based on a preset air suction amount; Based on the preset collection flow rate and the preset collection volume, a reagent collection operation is performed.

6. The method according to claim 4, characterized in that: After the step of feeding back abnormal information to a display device if the target acquisition pressure value is not within the preset acquisition pressure range, the method further includes: When receiving the instruction to end the collection operation, cleaning the pipetting needle; Moving the pipette needle to a preset dispensing point corresponding to the preset collection point to perform an analysis liquid dispensing operation, wherein the analysis liquid includes a reagent and a sample; When the instruction for dispensing the analysis liquid is received, the pipetting needle is cleaned.

7. A reagent collection monitoring device, characterized in that: The device comprises: A state detection module, used for determining a target collection pressure value of a pressure sensor disposed between a pipetting needle and a quantitative power device after the collection operation is started, wherein the pressure value collected by the pressure sensor is different when the collection object is a reagent or a gas, and the pipetting needle is a reagent needle or a sampling needle; A pressure comparison module, used to determine whether the target collection pressure value is within a preset collection pressure range corresponding to when the collection object is a reagent; The information feedback module is used to feed back abnormal information to the display device if the target collection pressure value is not within the preset collection pressure range, so as to ensure the accurate quantitative collection of the reagent.

8. A reagent collection monitoring device, characterized in that: The device comprises: a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the computer program is configured to implement the steps of the reagent collection monitoring method according to any one of claims 1 to 6.

9. A storage medium, characterized in that: The storage medium is a computer-readable storage medium, and a computer program is stored on the storage medium. When the computer program is executed by a processor, the steps of the reagent collection monitoring method according to any one of claims 1 to 6 are implemented.

10. A computer program product, characterized in that The computer program product comprises a computer program, and when the computer program is executed by a processor, the steps of the reagent collection monitoring method according to any one of claims 1 to 6 are implemented.

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