Permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens and preparation method of permeable basement membrane anti-aging lip care composition

Abstract

The invention relates to the technical field of lip cosmetics with the international classification number of A61K8, in particular to a permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens and a preparation method of the permeable basement membrane anti-aging lip care composition. According to the present invention, the multi-type recombinant collagen, the polyol, the vegetable oil, the penetration enhancer comprising azone, PPG-3-laurinol polyether-12 and PEG-10 soybean sterol, the plant extract prepared by carrying out decoction, enzymolysis, ethanol aqueous solution extraction and extraction on the ganoderma lucidum extract, the African melia azedarach bark, the licorice root and the centella asiatica, the thickening agent and the water are adopted; the composition obtained by the invention can effectively promote moisturizing and prevent lip aging, has efficient permeability, can effectively improve the resistance of the lip to the irritation brought by an external allergen or stimulus, has a good experience effect, can effectively moisten and moisturize the lip skin, and simultaneously endows the lip with a non-tight effect.

Description

Technical Field

[0001] The present invention relates to the technical field of lip cosmetics, with an international classification number of A61 K8, and more specifically to a permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens and a preparation method thereof. Background Art

[0002] Lip care cosmetics mainly include lip balm, lip mask, lip oil and lip scrub, etc. The main function of these products is to nourish and repair the lips.

[0003] Lip balm usually contains ingredients such as oil, wax and pigment, which can provide moisturizing and protection for the lips. Lip mask is a product used to deeply nourish and repair the lips. It usually contains rich nutrients and can provide long-term moisturizing and repair. Lip oil is a lip care product that provides moisture and gloss. It has a high moisturizing effect and can provide long-term protection for the lips. Lip scrub is used to remove dead skin and cuticles from the lips to help restore the smoothness and health of the lips. It usually contains fine particles that can gently remove dead skin.

[0004] There are many varieties of existing lip cosmetics, and the quality varies. Many cosmetics of this type, while pursuing multi-functions, often have the defect of different functional strengths. At the same time, due to the use of a large number of chemical raw materials, active ingredients, macromolecular peptides, etc. in this type of cosmetics, they have the defect of poor penetration and delivery to the skin. Therefore, providing a lip cosmetic that can effectively promote moisturizing and prevent lip aging, and has high permeability and can effectively enhance the lip resistance to irritation caused by external allergens or irritants is one of the topics that need to be studied at present. Summary of the invention

[0005] In view of the above technical problems, the inventors proposed the technical solution of the present invention, which specifically relates to a permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens and a preparation method thereof.

[0006] The invention provides a permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens, which comprises the following raw materials in parts by mass: 0.05-1 parts of recombinant collagen, 8-15 parts of polyols, 4-7 parts of vegetable oils, 2-4 parts of penetration enhancers, 0.1-2 parts of plant extracts, 0.1-0.8 parts of thickeners and 75-100 parts of water.

[0007] Preferably, the recombinant collagen includes recombinant humanized collagen type I, recombinant humanized collagen type III, recombinant humanized collagen type VI, recombinant humanized collagen type VII and recombinant humanized collagen type XVII. The above recombinant collagens are referred to as recombinant collagen type I, type III, type VI, type VII and type XVII, respectively, and the mass ratio is (0.5-2): (0.5-2): (0.5-2): (0.5-2): (0.5-2).

[0008] Preferably, the polyol includes at least one of sorbitol, ethylene glycol, propylene glycol, butylene glycol and glycerol.

[0009] Preferably, the vegetable oil includes at least one of grapeseed oil, jojoba oil, camellia oil, evening primrose oil, rapeseed oil, lemon oil, avocado oil, olive oil, almond oil, palm oil, and sweet almond oil.

[0010] Preferably, the penetration enhancer includes 0.2-0.5 parts of azone, 0.9-1.2 parts of PPG-3-laureth-12 and 0.9-1.3 parts of PEG-10 soy sterol.

[0011] Preferably, the preparation method of the plant extract comprises decocting, enzymolysis, ethanol-water extraction and extraction of Ganoderma lucidum extract, African china bark, liquorice and Centella asiatica.

[0012] Preferably, the method for preparing the plant extract comprises:

[0013] S1: grind 0.2-1 parts of Ganoderma lucidum, 0.1-0.8 parts of African neem bark, 0.2-0.8 parts of liquorice, and 0.2-0.9 parts of Centella asiatica according to weight, add 8-30 times the weight of the grinded materials of water, boil and stir at 60-80° C. for 2-6 hours, cool, and obtain product 1;

[0014] S2: Add 400-700 u / g of serrapeptase to product 1, perform enzymolysis at 35-55°C for 3-6 hours, inactivate the enzyme, and obtain product 2;

[0015] S3: Add anhydrous ethanol to the product 2, adjust the ethanol concentration to 15-35wt%, stir at 40-60°C for 2.5-5 hours, and concentrate to 0.8-1.2 times the mass of the crushed material to obtain the product 3;

[0016] S4: Add 15-25 times the mass of the extractant to the product three, extract at 40-50° C. for 3-5 hours, take the extracted water phase, concentrate, and dry to obtain a plant extract.

[0017] Preferably, the extractant is composed of an aqueous phase and an oil phase in a mass ratio of 2-3:1, the oil phase is ethyl acetate, and the aqueous phase is a calcium chloride aqueous solution with a mass concentration of 0.2-0.5%.

[0018] Preferably, the thickener comprises at least one of vegetable gum, polyacrylate and its derivatives, maleic acid copolymer, acrylic acid copolymer, cellulose and its derivatives.

[0019] Preferably, the composition further comprises an auxiliary agent, wherein the auxiliary agent comprises at least one of a preservative, a chelating agent, a pigment and a pH regulator.

[0020] Preferably, the dosage of the auxiliary agent is 0.05-1 part.

[0021] The present invention also provides a method for preparing the permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens, comprising the following steps:

[0022] Step 1: weigh the raw materials;

[0023] Step 2: Heat the vegetable oil and polyol, stir and mix to obtain an oil phase;

[0024] Step 3: Heat the penetration enhancer and water, stir and mix, and obtain an aqueous phase;

[0025] Step 4: Stir and mix the oil phase and the water phase, cool, add the remaining raw materials, continue stirring, and sterilize to obtain the permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens.

[0026] Beneficial effects of the present invention:

[0027] The present invention adopts polytype recombinant collagen, polyol, vegetable oil, a penetration enhancer composed of azone, PPG-3-laureth-12 and PEG-10 soybean sterol, a plant extract prepared by decocting, enzymolysis, ethanol aqueous solution extraction and extraction of ganoderma lucidum extract, neem bark, liquorice and Centella asiatica, a thickener and water, and prepares a lip composition together. The composition obtained by the present invention can effectively promote moisturizing and prevent lip aging, has high permeability and can effectively enhance the lips to resist the irritation caused by external allergens or irritants.

[0028] The composition prepared by the present invention has a good effect of inhibiting inflammation, can effectively reduce the inflammation or swelling caused by the environment or irritants to the lip skin, and give the lip skin a soothing and comfortable effect.

[0029] The use of PG-3-laureth-12 and PEG-10 soy sterol in the composition of the present invention can effectively promote the effect of inhibiting swelling. The two can synergistically enhance the skin soothing effect of the lip composition. The combination of the two can effectively promote the penetration and delivery of active ingredients to the skin, thereby giving the composition a better effect.

[0030] The present invention adopts the process of decoction, enzymolysis, ethanol aqueous solution extraction and extraction to effectively promote the anti-inflammatory effect of plant extracts. When preparing plant extracts, the present invention adopts a specific enzymolysis process to improve the anti-inflammatory effect of the extract to a certain extent, giving the composition a better skin soothing effect. The African neem bark and Centella asiatica used in the present invention can produce a compounding effect, better improving the effect of the plant extract on the composition to inhibit inflammation. The present invention adopts an extractant composed of calcium chloride aqueous solution and ethyl acetate, which can effectively improve the effect of plant extracts, wherein the selection of calcium chloride solution can effectively improve the dissolution and enrichment of anti-inflammatory active ingredients, so that the extract has a better anti-inflammatory effect, and ethyl acetate can make the organic phase enrich and extract impurities or oily components to a greater extent, so that the concentration of active ingredients in the plant extract obtained by drying the aqueous phase after extraction is higher, and the extract has a better anti-inflammatory effect.

[0031] The composition of the invention has a good experience effect, can effectively moisturize and hydrate the lip skin, and at the same time give the lips a non-tightening effect. DETAILED DESCRIPTION

[0032] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0033] Recombinant humanized collagen type I: Jiangsu Chuangjian Medical Technology Co., Ltd. Recombinant humanized collagen type III: Jiangsu Chuangjian Medical Technology Co., Ltd. Recombinant humanized collagen type VI: Shanghai Chunmai Biotechnology Co., Ltd. Recombinant humanized collagen type VI I: Shanghai Kanglang Biotechnology Co., Ltd. Recombinant humanized collagen type XVI I: Jiangsu Chuangjian Medical Technology Co., Ltd. PEG-10 soybean sterol: Hubei Hanwei Chemical Co., Ltd.

[0034] Composition 1:

[0035] The preparation method of the composition is as follows:

[0036] Step 1: Weigh the raw materials, mass parts: 0.2 parts of recombinant collagen (recombinant type I humanized collagen, recombinant type I II humanized collagen, recombinant type VI humanized collagen, recombinant type VI I humanized collagen and recombinant type XVIII humanized collagen are mixed in equal mass), 5 parts of 1,2-propylene glycol, 5 parts of glycerol, 2 parts of grape seed oil, 2 parts of jojoba oil, 1.5 parts of camellia oil, 1 part of evening primrose oil, 0.3 parts of azone, 1 part of PPG-3-laureth-12 and 1.2 parts of PEG-10 soybean sterol, 1.5 parts of plant extract, 0.5 parts of thickener carbomer-941 and 80 parts of water.

[0037] Step 2: Heat the vegetable oil (grape seed oil, jojoba oil, camellia oil, evening primrose oil) and polyol (1,2-propylene glycol, glycerol) to 68° C., stir and mix at 80 rpm for 0.5 hour to obtain an oil phase;

[0038] Step 3: heating the penetration enhancer (azone, PPG-3-laureth-12 and PEG-10 soy sterol) and water to 50° C., stirring and mixing at 70 rpm for 0.3 hours to obtain an aqueous phase;

[0039] Step 4: Mix the oil phase and the water phase by stirring at 70 rpm for 0.5 hour, cool to 40° C., add the remaining raw materials, continue stirring at the same speed for 0.2 hour, sterilize, and obtain a composition.

[0040] The preparation method of the plant extract used above is as follows:

[0041] S1: 0.7 parts of Ganoderma lucidum, 0.6 parts of African neem bark, 0.5 parts of liquorice, and 0.6 parts of Centella asiatica were crushed, 20 times the weight of water was added, and the mixture was boiled at 78°C and stirred at 60 rpm for 4.5 hours, and cooled to 40°C to obtain product 1;

[0042] S2: Add 500u / g serrapeptase to product 1, perform enzymolysis at 45℃, stir at 60rpm for 4.5 hours, inactivate the enzyme, and obtain product 2;

[0043] S3: Add anhydrous ethanol to the product 2 to adjust the ethanol concentration to 20wt%, stir at 50°C for 4 hours, and concentrate at 50°C under reduced pressure to 1 times the mass of the pulverized material to obtain product 3;

[0044] S4: Add 20 times the mass of extractant to product 3, extract at 48°C for 4 hours, take the extracted water phase, concentrate at 58°C under reduced pressure to 1 times the mass of the pulverized material, and freeze-dry at -42°C to a water content of 2.05wt%, to obtain a plant extract.

[0045] The extractant is composed of an aqueous phase and an oil phase in a mass ratio of 2.5:1, the oil phase is ethyl acetate, and the aqueous phase is a calcium chloride aqueous solution with a mass concentration of 0.3%.

[0046] Composition 2:

[0047] Step 1: Weigh the raw materials, mass parts: 0.2 parts of recombinant collagen (recombinant type I humanized collagen, recombinant type I II humanized collagen, recombinant type VI humanized collagen, recombinant type VI I humanized collagen and recombinant type XVIII humanized collagen are mixed in equal mass), 3 parts of 1,2-propylene glycol, 7 parts of glycerol, 1.5 parts of grape seed oil, 2.5 parts of jojoba oil, 1 part of camellia oil, 1.5 parts of evening primrose oil, 0.2 parts of azone, 1.1 parts of PPG-3-laureth-12 and 1.3 parts of PEG-10 soybean sterol, 1.45 parts of plant extract, 0.4 parts of thickener carbomer-941 and 78.5 parts of water.

[0048] Step 2: Heat the vegetable oil (grape seed oil, jojoba oil, camellia oil, evening primrose oil) and polyol (1,2-propylene glycol, glycerol) to 68° C., stir and mix at 80 rpm for 0.5 hour to obtain an oil phase;

[0049] Step 3: heating the penetration enhancer (azone, PPG-3-laureth-12 and PEG-10 soy sterol) and water to 50° C., stirring and mixing at 70 rpm for 0.3 hours to obtain an aqueous phase;

[0050] Step 4: Mix the oil phase and the water phase, cool to 40°C, add the remaining raw materials, sterilize, and obtain a composition.

[0051] The preparation method of the plant extract used above is as follows:

[0052] S1: 0.5 parts of Ganoderma lucidum, 0.7 parts of African neem bark, 0.4 parts of liquorice, and 0.7 parts of Centella asiatica were crushed, 25 times the weight of water was added, boiled at 80°C, stirred at 55 rpm for 4 hours, and cooled to 40°C to obtain product 1;

[0053] S2: Add 550u / g of serrapeptase to product 1, perform enzymolysis at 40℃ and stir at 60rpm for 4 hours, inactivate the enzyme, and obtain product 2;

[0054] S3: Add anhydrous ethanol to the product 2 to adjust the ethanol concentration to 35wt%, stir at 50°C for 4.5 hours, and concentrate at 50°C under reduced pressure to 1 times the mass of the crushed material to obtain product 3;

[0055] S4: Add 25 times the mass of extractant to product three, extract at 45°C for 4.2 hours, take the extracted water phase, concentrate at 58°C under reduced pressure to 1 times the mass of the crushed material, and freeze-dry at -42°C to a water content of 2.11wt%, to obtain a plant extract.

[0056] The extractant is composed of an aqueous phase and an oil phase in a mass ratio of 3:1, the oil phase is ethyl acetate, and the aqueous phase is a calcium chloride aqueous solution with a mass concentration of 0.5%.

[0057] Composition 3:

[0058] Compared with composition 1, it does not contain 1 part of PPG-3-laureth-12 and 1.2 parts of PEG-10 soy sterol, and the amount thereof can be supplemented with water. The rest is the same as composition 1.

[0059] Composition 4:

[0060] Compared with the composition 1, PPG-3-laureth-12 was omitted and the amount of PEG-10 soybean sterol was changed to 2.2 parts. The rest was the same as composition 1.

[0061] Composition 5:

[0062] Compared with the composition, PEG-10 soybean sterol was omitted and the amount of PPG-3-laureth-12 was changed to 2.2 parts. The rest was the same as composition 1.

[0063] Composition 6:

[0064] The difference from composition 1 is that the preparation method of the plant extract is different, specifically:

[0065] The preparation method of plant extract is as follows:

[0066] S1: 0.7 parts of Ganoderma lucidum, 0.6 parts of African neem bark, 0.5 parts of liquorice, and 0.6 parts of Centella asiatica were crushed, 20 times the weight of water was added, and the mixture was boiled at 78°C and stirred at 60 rpm for 4.5 hours, and cooled to 40°C to obtain product 1;

[0067] S3: Add anhydrous ethanol to the product 1 to adjust the ethanol concentration to 20wt%, stir at 50℃ for 4 hours, and concentrate at 50℃ under reduced pressure to 1 times the mass of the pulverized material to obtain product 2;

[0068] S4: Add 20 times the mass of extractant to the product 2, extract at 48°C for 4 hours, take the extracted water phase, concentrate at 58°C under reduced pressure to 1 times the mass of the pulverized material, and freeze-dry at -42°C to a water content of 2.05wt%, to obtain a plant extract.

[0069] The extractant is composed of an aqueous phase and an oil phase in a mass ratio of 2.5:1, the oil phase is ethyl acetate, and the aqueous phase is a calcium chloride aqueous solution with a mass concentration of 0.3%.

[0070] Composition 7:

[0071] The difference from composition 1 is that the preparation method of the plant extract is different, specifically:

[0072] The preparation method of plant extract is as follows:

[0073] S1: Grind 0.7 parts of Ganoderma lucidum, 0.5 parts of Licorice, and 1.2 parts of Centella asiatica, add 20 times the weight of the ground materials of water, boil at 78°C, stir at 60rpm for 4.5 hours, and cool to 40°C to obtain product 1;

[0074] S3: Add anhydrous ethanol to the product 1 to adjust the ethanol concentration to 20wt%, stir at 50℃ for 4 hours, and concentrate at 50℃ under reduced pressure to 1 times the mass of the pulverized material to obtain product 2;

[0075] S4: Add 20 times the mass of extractant to the product 2, extract at 48°C for 4 hours, take the extracted water phase, concentrate at 58°C under reduced pressure to 1 times the mass of the pulverized material, and freeze-dry at -42°C to a water content of 2.05wt%, to obtain a plant extract.

[0076] The extractant is composed of an aqueous phase and an oil phase in a mass ratio of 2.5:1, the oil phase is ethyl acetate, and the aqueous phase is a calcium chloride aqueous solution with a mass concentration of 0.3%.

[0077] Composition 8:

[0078] The difference from composition 1 is that the preparation method of the plant extract is different, specifically:

[0079] The preparation method of plant extract is as follows:

[0080] S1: 0.7 parts of Ganoderma lucidum, 1.2 parts of African neem bark, and 0.5 parts of liquorice were crushed, 20 times the weight of water was added, boiled at 78°C, stirred at 60rpm for 4.5 hours, cooled to 40°C, and product 1 was obtained;

[0081] S2: Add 500u / g serrapeptase to product 1, perform enzymolysis at 45℃, stir at 60rpm for 4.5 hours, inactivate the enzyme, and obtain product 2;

[0082] S3: Add anhydrous ethanol to the product 2 to adjust the ethanol concentration to 20wt%, stir at 50°C for 4 hours, and concentrate at 50°C under reduced pressure to 1 times the mass of the pulverized material to obtain product 3;

[0083] S4: Add 20 times the mass of extractant to product 3, extract at 48°C for 4 hours, take the extracted water phase, concentrate at 58°C under reduced pressure to 1 times the mass of the pulverized material, and freeze-dry at -42°C to a water content of 2.05wt%, to obtain a plant extract.

[0084] The extractant is composed of an aqueous phase and an oil phase in a mass ratio of 2.5:1, the oil phase is ethyl acetate, and the aqueous phase is a calcium chloride aqueous solution with a mass concentration of 0.3%.

[0085] Composition 9:

[0086] The difference from composition 1 is that the preparation method of the plant extract is different, specifically:

[0087] The preparation method of plant extract is as follows:

[0088] S1: 0.7 parts of Ganoderma lucidum, 0.6 parts of African neem bark, 0.5 parts of liquorice, and 0.6 parts of Centella asiatica were crushed, 20 times the weight of water was added, and the mixture was boiled at 78°C and stirred at 60 rpm for 4.5 hours, and cooled to 40°C to obtain product 1;

[0089] S2: Add 500u / g serrapeptase to product 1, perform enzymolysis at 45℃, stir at 60rpm for 4.5 hours, inactivate the enzyme, and obtain product 2;

[0090] S3: Add anhydrous ethanol to the product 2 to adjust the ethanol concentration to 20wt%, stir at 50°C for 4 hours, and concentrate at 50°C under reduced pressure to 1 times the mass of the pulverized material to obtain product 3;

[0091] S4: Add 20 times the mass of extractant to product 3, extract at 48°C for 4 hours, take the extracted water phase, concentrate at 58°C under reduced pressure to 1 times the mass of the pulverized material, and freeze-dry at -42°C to a water content of 2.05wt%, to obtain a plant extract.

[0092] The extractant is composed of an aqueous phase and an oil phase in a mass ratio of 2.5:1, the oil phase is ethyl acetate, and the aqueous phase is water.

[0093] Composition 10:

[0094] The difference from composition 1 is that the preparation method of the plant extract is different, specifically:

[0095] The preparation method of plant extract is as follows:

[0096] S1: 0.7 parts of Ganoderma lucidum, 0.6 parts of African neem bark, 0.5 parts of liquorice, and 0.6 parts of Centella asiatica were crushed, 20 times the weight of water was added, and the mixture was boiled at 78°C and stirred at 60 rpm for 4.5 hours, and cooled to 40°C to obtain product 1;

[0097] S2: Add 500u / g serrapeptase to product 1, perform enzymolysis at 45℃, stir at 60rpm for 4.5 hours, inactivate the enzyme, and obtain product 2;

[0098] S3: Add anhydrous ethanol to the product 2 to adjust the ethanol concentration to 20wt%, stir at 50°C for 4 hours, and concentrate at 50°C under reduced pressure to 1 times the mass of the pulverized material to obtain product 3;

[0099] S4: Add 20 times the mass of extractant to product 3, extract at 48°C for 4 hours, take the extracted water phase, concentrate at 58°C under reduced pressure to 1 times the mass of the pulverized material, and freeze-dry at -42°C to a water content of 2.05wt%, to obtain a plant extract.

[0100] The extractant is composed of a water phase and an oil phase in a mass ratio of 2.5:1, the oil phase is cyclohexane, and the water phase is a calcium chloride aqueous solution with a mass concentration of 0.3%.

[0101] Experimental test:

[0102] 1. Patch test

[0103] The above composition was diluted with PBS buffer at pH=7 to a concentration of 100 mg / mL as a test sample.

[0104] Healthy skin volunteers aged 30-45 were recruited and randomly divided into 5 males and 5 females in each group. 0.05 mL of the test sample was taken with a syringe and placed in the spot tester. The spot test tape was applied to the skin of the volunteer's left forearm, and air was expelled by light pressure to evenly apply the sample. After 24 hours, the skin irritation results were evaluated according to the evaluation criteria recorded in Table 1. The results are shown in Table 2.

[0105] Table 1: Evaluation criteria

[0106]

[0107] Table 2: Test results

[0108]

[0109]

[0110] 2. Experimental test on inhibiting inflammation

[0111] SPF healthy Wi star rats weighing 230±10g were selected and adaptively raised for 5 days. The rats were randomly divided into a blank group, a positive drug group (positive drug was compound dexamethasone acetate cream, 999 Piyanping, China Resources Sanjiu Pharmaceutical Co., Ltd.) and combination groups 1-10, a total of 12 groups, 10 rats in each group. In the positive drug group, the positive drug was applied to the right ear of the rat every day, 30 mg each time, once at 9:00 am every day; in the combination group, 50 mg of the combination was applied to the right ear of the rat every day, once at 9:00 am and 8:00 pm every day; the blank group was applied once at 9:00 am every day with 50 mg of deionized water; the drug was administered for 10 consecutive days, with free drinking water and diet. On the 11th day of administration, 1 hour after administration at 9:00 am, the right ear of each rat in each group was exposed and 50 μL of xylene was dripped, and the left ear was not inflamed as a control. Half an hour after the inflammation was induced, the rats were killed by dislocating the neck, and a 6 mm diameter punch was used to punch a circular ear piece along the auricle at the same position of the left and right ears, and the swelling degree and swelling inhibition rate of the rat ears were calculated. The results are shown in Table 3. Wherein, swelling degree = right ear weight - left ear weight; swelling inhibition rate / % = (average swelling degree of the blank group - average swelling degree of the drug group) / average swelling degree of the blank group × 100%. The test results are shown in Table 2.

[0112] Table 2: Inflammation inhibition test

[0113]

[0114] According to the test in Table 1-2, it can be seen that the composition prepared by the present invention has a good effect of inhibiting inflammation, can effectively reduce the inflammation or swelling of the lip skin caused by the environment or irritants, and give the lip skin a soothing and comfortable effect.

[0115] According to the test results of compositions 1 and 3-5, the use of PG-3-laureth-12 and PEG-10 soy sterol in the composition of the present invention can effectively promote the swelling inhibition effect, and the two can synergistically enhance the skin soothing effect of the lip composition. The combination of the two can effectively promote the penetration and delivery of active ingredients to the skin, thereby giving the composition a better effect.

[0116] According to the test of composition 1, 6-10, it can be known that the process of decoction, enzymolysis, ethanol aqueous solution extraction and extraction of the present invention can effectively promote the anti-inflammatory effect of plant extracts. According to the test of composition 1, 6, it can be known that when preparing plant extracts, the present invention adopts a specific enzymolysis process to improve the anti-inflammatory effect of the extract to a certain extent, giving the composition a better skin soothing effect. According to the test of composition 1, 7-8, it can be known that African neem bark and Centella asiatica can produce a compounding effect, which better improves the effect of plant extracts on the composition to inhibit inflammation. The present invention uses an extractant composed of calcium chloride aqueous solution and ethyl acetate, which can effectively improve the effect of plant extracts, wherein the selection of calcium chloride solution can effectively improve the dissolution and enrichment of anti-inflammatory active ingredients, so that the extract has a better anti-inflammatory effect, and ethyl acetate can make the organic phase enrich and extract impurities or oiliness to a greater extent, so that the concentration of active ingredients in the plant extract obtained by drying the aqueous phase after extraction is higher, and the extract has a better anti-inflammatory effect.

[0117] 3. Experience test

[0118] 15 volunteers aged 35-45 were selected and divided into 3 groups, 5 people in each group. Each group of volunteers applied 0.3g of the above composition 1-3 to the lips, and scored the moisturizing and skin feel effects of the lips 2 hours later. The highest score was 10 points; among them, the moisturizing score standard was that the more moisturized and not dry the lips were, the higher the score was; the skin feel score standard was that the lips were not tight and the more comfortable they were, the higher the score was. The test results are shown in Table 4.

[0119] Table 4: Experience test

[0120]

[0121] According to Table 4, the composition of the present invention has a good experience effect, can effectively moisturize and hydrate the lip skin, and at the same time give the lips a less tight effect. However, composition 3 does not contain PPG-3-laureth-12 and PEG-10 soy sterol, resulting in poor dispersion and dissolution of the active ingredients of the composition, and significantly reduced moisturizing and skin feel effects.

[0122] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens, characterized in that: The invention comprises the following raw materials in parts by mass: 0.05-1 parts of recombinant collagen, 8-15 parts of polyol, 4-7 parts of vegetable oil, 2-4 parts of penetration enhancer, 0.1-2 parts of plant extract, 0.1-0.8 parts of thickener and 75-100 parts of water.

2. The permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that: The recombinant collagen includes recombinant collagen type I, type III, type VI, type VII and type XVII.

3. The permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that: The polyol includes at least one of sorbitol, ethylene glycol, propylene glycol, butylene glycol and glycerol.

4. The permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that: The vegetable oil includes at least one of grapeseed oil, jojoba oil, camellia oil, evening primrose oil, rapeseed oil, lemon oil, avocado oil, olive oil, almond oil, palm oil, and sweet almond oil.

5. The permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that: The penetration enhancer includes 0.2-0.5 parts of azone, 0.9-1.2 parts of PPG-3-laureth-12 and 0.9-1.3 parts of PEG-10 soybean sterol.

6. The permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that: The preparation method of the plant extract comprises the steps of decocting, enzymolysis, ethanol-water solution extraction and extraction of ganoderma lucidum extract, chinaberry bark, liquorice and centella asiatica.

7. The permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that: The thickener includes at least one of vegetable gum, polyacrylate and its derivatives, maleic acid copolymer, acrylic acid copolymer, cellulose and its derivatives.

8. The permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 1, characterized in that: The invention also comprises auxiliary agents, which include at least one of preservatives, chelating agents, pigments and pH regulators.

9. The permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to claim 8, characterized in that: The dosage of the additive is 0.05-1 part.

10. A method for preparing the permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens according to any one of claims 1 to 9, characterized in that: The steps include: Step 1: weigh the raw materials; Step 2: Heat the vegetable oil and polyol, stir and mix to obtain an oil phase; Step 3: Heat the penetration enhancer and water, stir and mix to obtain an aqueous phase; Step 4: Stir and mix the oil phase and the water phase, cool, add the remaining raw materials, continue stirring, and sterilize to obtain the permeable basement membrane anti-aging lip care composition containing multiple recombinant collagens.

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