Probiotic skin repair composite dressing and preparation method thereof

Abstract

The invention belongs to the field of preparation of skin repair dressings, and particularly relates to a probiotic skin repair composite dressing and a preparation method thereof. The preparation method comprises the following steps: grinding heterostichopus japonicus, liverwort and festuca arundinacea into thick liquid to obtain a nutrient solution, culturing a probiotic agent, synergistically enhancing the skin repair effect, carrying out grafting reaction on a polypropylene imine triamine dendritic polymer and kaempferol-3-O-rutinoside to load a repair-promoting suspension, spraying an anti-oxidation film-forming solution on the surfaces of repair-promoting nano particles, and carrying out film-forming treatment on the surfaces of the repair-promoting nano particles. The obtained probiotic-containing repair promoting agent efficiently and continuously exerts a repair promoting effect; after the polytetramethylene ether glycol and the cellulose nanocrystals are combined, the mixture and the waterborne polyurethane are subjected to a cross-linking reaction, the obtained composite dressing adsorbs the repair promoting composition, and the prepared wound repairing elastic matrix is high in flexibility; the repair-promoting compound matrix is mixed with the probiotic-containing repair-promoting agent, and the prepared probiotic skin repair compound dressing can efficiently repair skin, and is high in flexibility and comfortable to use.

Description

Technical Field

[0001] The invention belongs to the field of skin repair dressing preparation, and specifically refers to a probiotic skin repair composite dressing and a preparation method thereof. Background Art

[0002] Skin repair dressing is a medical dressing used to treat and repair skin damage. It mainly helps the skin recover by providing protection, promoting healing and reducing infection. Skin repair dressings can be divided into many types, such as transparent dressings, hydrogel dressings, silicone dressings, liquid dressings, etc. These dressings are usually composed of medical glue, medical gauze, bioactive ingredients or polymer materials.

[0003] Skin repair dressings can cover the injured skin surface to form a physical barrier to prevent external bacteria, dust and other contaminants from entering the wound, reducing the risk of infection. They can also prevent friction and external irritation, relieve pain and discomfort. Skin repair dressings accelerate wound healing by keeping the wound moist and providing a good environment for cell growth and regeneration. Some dressings also contain drug ingredients, such as antibacterial drugs, growth factors or cytokines, which can further promote wound healing.

[0004] At present, the existing skin repair dressing preparation technology has the following problems: First, poor absorption. Traditional dressings such as gauze and vaseline gauze have limited absorption capacity and are difficult to meet the needs of long-term repair. The dressings need to be changed frequently and are easy to adhere to the wound surface. When changing, secondary mechanical damage may be caused and the healing time may be prolonged. Second, poor flexibility. The existing skin repair dressings have poor mechanical properties, are brittle, and are difficult to adapt to dynamic environments. Third, the existing skin repair dressings are easy to breed bacteria, have poor biocompatibility, are difficult to effectively degrade in the body, and long-term use will induce inflammatory reactions. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a probiotic skin repair composite dressing and a preparation method thereof. In order to solve the problems of poor absorbency, long skin healing time, poor flexibility and inflammation caused by long-term use of the existing skin repair dressings, the present invention uses a wound repair elastic matrix, a moisturizing care solution, and a probiotic repair-promoting agent to mix. The prepared probiotic skin repair composite dressing has strong absorbency, effectively promotes skin healing, has excellent flexibility, is non-irritating to long-term use, and will not cause inflammatory reactions.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: the present invention proposes a probiotic skin repair composite dressing, and the raw materials for preparing the probiotic skin repair composite dressing specifically include the following components in parts by weight: It contains 28-37 parts of probiotic repair-promoting agent, 105-112 parts of wound repair elastic matrix, 5-7 parts of gentian extract, 4-8 parts of kava root extract, and 2.9-4.6 parts of dihydrooat alkaloids.

[0007] Preferably, the raw materials for preparing the probiotic repair promoting agent include the following components in parts by weight: 1.5-2.4 parts of probiotic agents, 6-9 parts of Stipa isocarpa, 10-12 parts of liverwort, 7-11 parts of fescue, 2.1-2.8 parts of coral lactone, 1.9-2.6 parts of hydroxytyrosol, 2.1-3.4 parts of N-acetyl-L-hydroxyproline, 2.5-3 parts of L-alanyl-L-glutamine, 17-21 parts of polypropylene imine triamine dendrimer, 14-19 parts of kaempferol-3-O-rutinoside, 1.2-1.4 parts of 4-N,N-dimethylpyridine, 8-12 parts of polyglutamic acid, and 9-11 parts of polyvinyl pyrrolidone.

[0008] Preferably, the raw materials for preparing the wound repair elastic matrix include the following components in parts by weight: 35-42 parts of polytetramethylene ether glycol, 33-45 parts of cellulose nanocrystals, 40-52 parts of water-based polyurethane, 14-18 parts of carbodiimide, 2.4-2.7 parts of bismorpholinyl diethyl ether, 4-6 parts of benzoic acid, 8-12 parts of hydrolyzed sponge microneedles, 23-31 parts of elastin peptides, and 14-17 parts of acrylic resin.

[0009] Preferably, the preparation method of the probiotic-containing repair-promoting agent specifically comprises the following steps: S1. Put Stipa indica, Marchantia polyphylla and Festuca australis into a pulping machine with a power of 1.8-2.4 kW, the pulping temperature is 30-36°C, the pulping time is 35-40 min, the pulping speed is 18000-20000 r / min, and the pulping is performed. After the pulping, it is filtered with a sterile filter cloth to obtain a nutrient solution; S2, the probiotic agent, the nutrient solution prepared in S1, and the 5% sterile glucose solution are placed in a constant temperature shaking incubator with a power of 2.2-2.5 kW, the constant temperature shaking culture temperature is 30°C, the constant temperature shaking culture time is 28-32 h, the constant temperature shaking culture speed is 210-230 r / min, and after the constant temperature shaking culture, the probiotic agent, the nutrient solution prepared in S1, and the 5% sterile glucose solution are placed in a constant temperature shaking incubator with a power of 1.5-1.8 kW, the stirring temperature is 25-33°C, the stirring speed is 300-320 r / min, the stirring time is 23-28 min, and the mixture is stirred to obtain a repair-promoting suspension; S3, placing polypropylene imine triamine dendrimer, kaempferol-3-O-rutinoside, 4-N,N-dimethylpyridine and 50% ethanol solution in a reactor with a power of 2.2-2.6 kW, the reaction temperature is 60-70°C, the reaction time is 18-24 h, the reaction speed is 80-110 r / min, and after grafting reaction, a composite solution is obtained; S4, placing polyglutamic acid, polyvinyl pyrrolidone and ultrapure water in a constant temperature stirrer with a power of 1.6-1.9 kW, the constant temperature stirring temperature is 35-40°C, the constant temperature stirring speed is 140-180 r / min, the constant temperature stirring time is 24-36 h, and the constant temperature stirring is performed to obtain an antioxidant film-forming solution; S5, putting the repair-promoting suspension prepared by S2 into the complex solution prepared by S3, stirring at a temperature of 28-35°C, stirring for 15-20 min, stirring at a speed of 350-380 r / min, mixing well, putting into a vacuum freeze-nano spray dryer with a power of 2.3-2.8 kW, vacuum degree of -0.05 MPa, freeze-nano spray drying temperature of -40°C, freeze-nano spray drying particle size of 100 nm, freeze-nano spray drying time of 2.5-3 h, freeze-spray drying to obtain repair-promoting nanoparticles; S6. Use a sprayer with a power of 1.6-2.3 kW, spray the antioxidant film-forming solution prepared in S4 evenly on the surface of the pro-repair nanoparticles prepared in S5, and place it at a temperature of 25-30°C for 13-18 minutes to obtain a pro-repair agent containing probiotics.

[0010] Further, in S2, the probiotic agent is composed of Lactobacillus plantarum, Staphylococcus aureus, and Corynebacterium stagnation, and the weight ratio is 0.8-1.1:1.1-1.2:1. The Lactobacillus plantarum was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.12934, the Staphylococcus aureus was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.8022, and the Corynebacterium stagnation was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.844. The solid-liquid ratio of the probiotic agent to 5% sterile glucose solution is 1:100 g / mL. In S3, the mass fraction of the polypropyleneimine triamine dendrimer in the 50% ethanol solution is 13-17%.

[0011] Furthermore, in S4, the mass fraction of the polyglutamic acid in ultrapure water is 2-4%.

[0012] Preferably, the method for preparing the wound repair elastic matrix specifically comprises the following steps: L1, polytetramethylene ether glycol, cellulose nanocrystals, anhydrous ethanol, and dimethyl sulfoxide are placed in a reactor with a power of 2.2-2.6 kW, the reaction temperature is 60°C, the reaction time is 18-24 h, and the reaction speed is 260-280 r / min, and modified to obtain an elastic composite solution; L2, the elastic composite solution prepared in L1, water-based polyurethane, carbodiimide, bismorpholinyl diethyl ether, ultrapure water, and ethyl acetate are placed in a constant temperature stirrer with a power of 1.6-1.9 kW, the constant temperature stirring temperature is 60-80°C, the constant temperature stirring speed is 600-800 r / min, the constant temperature stirring time is 6-8 h, and the constant temperature stirring is carried out to perform a cross-linking reaction to form a composite dressing with elasticity and strong hygroscopicity; L3, put benzoic acid, hydrolyzed sponge microneedles, elastin peptide, acrylic resin, and 50% ethanol solution into a stirrer with a power of 1.5-1.8 kW, stirring at a temperature of 60-80°C, a stirring speed of 200-280 r / min, and a stirring time of 1.8-2.4 h to mix and obtain a repair-promoting composition; L4, put the repair-promoting composition prepared by L3 into the composite dressing prepared by L2, stir at a temperature of 30-36°C, stir for 20-28 min, stir at a speed of 300-500 r / min, stir and mix, fully absorb, and obtain an elastic matrix for repairing wounds.

[0013] Furthermore, in L1, the mass fraction of the polytetramethylene ether glycol in anhydrous ethanol is 6-8%, the mass fraction of the cellulose nanocrystals in dimethyl sulfoxide is 3%, in L2, the mass fraction of the carbodiimide in ethyl acetate is 10%, and the mass fraction of the water-based polyurethane in ultrapure water is 35-42%.

[0014] Furthermore, in L3, the solid-liquid ratio of the benzoic acid to the 50% ethanol solution is 1:20 g / mL.

[0015] The present invention also proposes a method for preparing a probiotic skin repair composite dressing, which specifically comprises the following steps: Step 1, put the gentian extract, kava root extract, dihydroavenous alkaloids, and anhydrous ethanol into a stirrer with a power of 1.5-1.8 kW, stir at a temperature of 33-36° C., stir at a speed of 310-340 r / min, stir for 15-18 min, and stir to obtain a moisturizing care solution; Step 2: Put the wound repair elastic matrix into the moisturizing care solution prepared in step 1, with a stirring temperature of 25-33°C, a stirring speed of 300-400 r / min, and a stirring time of 15-20 min. After mixing, put it into a UV lamp sterilizer with a power of 1.4 kW, the UV lamp sterilization wavelength is 253.7 nm, the UV lamp sterilization temperature is 28°C, and the UV lamp sterilization time is 40 min. Sterilize with UV lamp to obtain a repair-promoting composite matrix; Step 3: Place the probiotic repair-promoting agent into the repair-promoting composite matrix prepared in step 2, use an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and obtain a probiotic skin repair composite dressing after uniform dispersion.

[0016] Preferably, in step 1, the mass fraction of the gentian extract in anhydrous ethanol is 3.2-4.8%.

[0017] The beneficial effects of the present invention are as follows: The present invention adopts a probiotic agent containing plant lactobacillus, staphylococcus xylinum and corynebacterium stagnatum, which can regulate the balance of skin microbial communities, reduce the release of inflammatory factors, promote the synthesis of collagen, and supplement the level of ceramide in the skin, thereby maintaining the homeostasis of the skin barrier, and accelerates skin re-epithelialization and promotes wound healing by stimulating the proliferation and migration of keratinocytes. The juice of Stipa indica, liverwort and fescue after grinding contains rich natural organic matter, which can provide carbon and nitrogen sources for bacteria and support their growth and metabolism. The nutrient solution also contains antioxidant and anti-inflammatory ingredients, which cooperate with the probiotic agent to enhance the effect of skin repair. N-acetyl-L-hydroxyproline and L-alanyl-L-glutamine can promote skin nerve regeneration, reduce the damage of oxidative stress to skin cells, moisturize and enhance skin barrier function, and promote the proliferation and differentiation of skin cells. Under the catalytic action of 4-N,N-dimethylpyridine, the amino groups in the polypropylene imine triamine dendrimer react with the hydroxyl groups in kaempferol-3-O-rutinoside to form amide bonds, thereby achieving grafting. The obtained complex solution has good biocompatibility, can efficiently load the repair-promoting suspension, and achieve the sustained release of the effective ingredients. After polyglutamic acid and polyvinyl pyrrolidone are mixed, they interact through hydrogen bonds to form antioxidant The film-forming solution is sprayed on the surface of the pro-repair nanoparticles. The pro-repair agent containing probiotics is easy to be absorbed by cells and can continuously and efficiently play a pro-repair role. The ether oxygen of polytetramethylene ether glycol and the hydroxyl group on the surface of cellulose nanocrystals can form a non-covalent bond. The flexibility of polytetramethylene ether glycol is combined with cellulose nanocrystals, so that the elastic composite has good mechanical properties and avoids brittle fracture. The water-based polyurethane has strong hygroscopicity. Under the action of carbodiimide and bismorpholinyl diethyl ether, a cross-linking reaction occurs between the water-based polyurethane and polytetramethylene ether glycol and cellulose nanocrystals to obtain a composite dressing. Benzoic acid has an antibacterial effect. The hydrolyzed sponge microneedles can be applied on the skin surface. A large number of tiny channels are formed on the surface, which significantly increases the permeability of the skin. The combination of elastin peptide and acrylic resin provides a good adhesion and growth environment for skin cells, thereby accelerating wound healing and avoiding the appearance of scars. The composite dressing fully absorbs the repair-promoting composition, and the obtained wound repair elastic matrix does not need to be replaced many times, which quickly promotes the absorption of effective components by skin cells; the moisturizing care solution has anti-inflammatory, sedative and soothing effects, and the mixed wound repair elastic matrix and the probiotic repair-promoting agent contain a probiotic skin repair composite dressing that is easily absorbed by skin cells, has efficient skin healing and repair, strong flexibility, strong hygroscopicity, will not cause inflammation after long-term use, and is comfortable to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and understandable manner in conjunction with the accompanying drawings. Obviously, the drawings described below are only for the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 This is a scanning electron microscope image of the internal structure of the composite dressing of the present invention; Figure 2 This is a graph showing the wound healing rate of mice described in Experimental Example 1 of the present invention; Figure 3 This is a graph showing the tensile strength results of the skin repair dressing described in Experimental Example 2 of the present invention; Figure 4 This is a graph showing the water absorption rate of the skin repair dressing described in Experimental Example 2 of the present invention; Figure 5 This is a graph showing the results of cytokine content in the supernatant of macrophages described in Experimental Example 3 of the present invention. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0021] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0022] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0023] Example 1: This example provides a probiotic skin repair composite dressing and a preparation method thereof. The raw materials for preparing the probiotic skin repair composite dressing specifically include the following components in parts by weight: Contains 28 parts of probiotic repair agent, 105 parts of wound repair elastic matrix, 5 parts of gentian extract, 4 parts of kava root extract, and 2.9 parts of dihydrooat alkaloids.

[0024] The raw materials for preparing the probiotic repair promoting agent include the following components in parts by weight: 1.5 parts of probiotic agents, 6 parts of Stipa isophylla, 10 parts of liverwort, 7 parts of fescue, 2.1 parts of coralline lactone, 1.9 parts of hydroxytyrosol, 2.1 parts of N-acetyl-L-hydroxyproline, 2.5 parts of L-alanyl-L-glutamine, 17 parts of polypropylene imine triamine dendrimer, 14 parts of kaempferol-3-O-rutinoside, 1.2 parts of 4-N,N-dimethylpyridine, 8 parts of polyglutamic acid, and 9 parts of polyvinyl pyrrolidone.

[0025] The raw materials for preparing the wound repair elastic matrix include the following components in parts by weight: 35 parts of polytetramethylene ether glycol, 33 parts of cellulose nanocrystals, 40 parts of waterborne polyurethane, 14 parts of carbodiimide, 2.4 parts of bismorpholinyl diethyl ether, 4 parts of benzoic acid, 8 parts of hydrolyzed sponge microneedles, 23 parts of elastin peptides, and 14 parts of acrylic resin.

[0026] The preparation method of the probiotic-containing repair-promoting agent specifically comprises the following steps: S1. Put Stipa indica, Marchantia polyphylla and Festuca australis into a pulping machine with a power of 1.8 kW, the pulping temperature is 30°C, the pulping time is 35 min, the pulping speed is 18000 r / min, and the pulping is performed. After the pulping, it is filtered with a sterile filter cloth to obtain a nutrient solution; S2, the probiotic agent, the nutrient solution prepared in S1, and the 5% sterile glucose solution were placed in a constant temperature shaking incubator with a power of 2.2 kW, the constant temperature shaking culture temperature was 30°C, the constant temperature shaking culture time was 28 h, the constant temperature shaking culture speed was 210 r / min, and after the constant temperature shaking culture, the probiotic agent was placed in a stirrer with a power of 1.5 kW, the stirring temperature was 25°C, the stirring speed was 300 r / min, the stirring time was 23 min, and the mixture was stirred to obtain a repair-promoting suspension; S3, placing polypropylene imine triamine dendrimer, kaempferol-3-O-rutinoside, 4-N,N-dimethylpyridine and 50% ethanol solution in a reactor with a power of 2.2 kW, the reaction temperature is 60°C, the reaction time is 18 h, the reaction speed is 80 r / min, and after grafting reaction, a composite solution is obtained; S4, putting polyglutamic acid, polyvinyl pyrrolidone and ultrapure water into a constant temperature stirrer with a power of 1.6 kW, the constant temperature stirring temperature is 35°C, the constant temperature stirring speed is 140 r / min, the constant temperature stirring time is 24 h, and the constant temperature stirring is performed to obtain an antioxidant film-forming solution; S5, put the repair-promoting suspension prepared by S2 into the complex solution prepared by S3, with the stirring temperature at 28°C, the stirring time at 15 min, and the stirring speed at 350 r / min. After mixing, put it into a vacuum freeze-nano spray dryer with a power of 2.3 kW, the vacuum degree at -0.05 MPa, the freeze-nano spray drying temperature at -40°C, the freeze-nano spray drying particle size at 100 nm, and the freeze-nano spray drying time at 2.5 h, and perform freeze-spray drying to obtain repair-promoting nanoparticles; S6. Use a 1.6 kW sprayer to evenly spray the antioxidant film-forming solution prepared in S4 onto the surface of the pro-repair nanoparticles prepared in S5. Place the solution at 25°C for 1.2 h to obtain a pro-repair agent containing probiotics.

[0027] In S2, the probiotic agent consists of Lactobacillus plantarum, Staphylococcus aureus, and Corynebacterium stagnation, with a weight ratio of 0.8:1.1:1. The Lactobacillus plantarum was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.12934, the Staphylococcus aureus was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.8022, and the Corynebacterium stagnation was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.844. The solid-liquid ratio of the probiotic agent to 5% sterile glucose solution is 1:100 g / mL. In S3, the mass fraction of the polypropyleneimine triamine dendrimer in the 50% ethanol solution is 13%.

[0028] In S4, the mass fraction of the polyglutamic acid in ultrapure water is 2%.

[0029] The preparation method of the wound repair elastic matrix specifically comprises the following steps: L1, polytetramethylene ether glycol, cellulose nanocrystals, anhydrous ethanol, and dimethyl sulfoxide were placed in a reactor with a power of 2.2 kW, the reaction temperature was 60°C, the reaction time was 18 h, and the reaction speed was 260 r / min, and the elastic composite solution was obtained; L2, the elastic composite solution prepared in L1, water-based polyurethane, carbodiimide, bismorpholinyl diethyl ether, ultrapure water, and ethyl acetate were placed in a constant temperature stirrer with a power of 1.6 kW, the constant temperature stirring temperature was 60°C, the constant temperature stirring speed was 600 r / min, the constant temperature stirring time was 6 h, and the constant temperature stirring was carried out to perform a cross-linking reaction to form a composite dressing with elasticity and strong hygroscopicity; L3, put benzoic acid, hydrolyzed sponge microneedles, elastin peptide, acrylic resin, and 50% ethanol solution into a 1.5 kW stirrer, stir at 60°C, stir at 200 r / min, and stir for 1.8 h to obtain a repair-promoting composition; L4. Place the repair-promoting composition prepared in L3 into the composite dressing prepared in L2, stir at a temperature of 30°C, for 20 min, at a speed of 300 r / min, stir and mix to allow for full adsorption to obtain an elastic matrix for repairing wounds.

[0030] In L1, the mass fraction of the polytetramethylene ether glycol in anhydrous ethanol is 6%, the mass fraction of the cellulose nanocrystals in dimethyl sulfoxide is 3%, in L2, the mass fraction of the carbodiimide in ethyl acetate is 10%, and the mass fraction of the waterborne polyurethane in ultrapure water is 35%.

[0031] In L3, the solid-liquid ratio of the benzoic acid to the 50% ethanol solution is 1:20 g / mL.

[0032] This embodiment also provides a method for preparing a probiotic skin repair composite dressing, which specifically comprises the following steps: Step 1: put gentian extract, kava root extract, dihydroavenous alkaloids, and anhydrous ethanol into a 1.5 kW stirrer, stir at a temperature of 33° C., stir at a speed of 310 r / min, and stir for 15 min to obtain a moisturizing care solution; Step 2: Put the wound repair elastic matrix into the moisturizing care solution prepared in step 1, with a stirring temperature of 25°C, a stirring speed of 300 r / min, and a stirring time of 15 min. After mixing, put it into a UV lamp sterilizer with a power of 1.4 kW, a UV lamp sterilization wavelength of 253.7 nm, a UV lamp sterilization temperature of 28°C, and a UV lamp sterilization time of 40 min. Sterilize with UV lamp to obtain a repair-promoting composite matrix; Step 3: Place the probiotic repair-promoting agent into the repair-promoting composite matrix prepared in step 2, use an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and obtain a probiotic skin repair composite dressing after uniform dispersion.

[0033] In step 1, the mass fraction of the gentian extract in anhydrous ethanol is 3.2%.

[0034] Example 2: This example provides a probiotic skin repair composite dressing and a preparation method thereof. The raw materials for preparing the probiotic skin repair composite dressing specifically include the following components in parts by weight: Contains 32 parts of probiotic repair agents, 109 parts of wound repair elastic matrix, 6 parts of gentian extract, 6 parts of kava root extract, and 3.4 parts of dihydrooat alkaloids.

[0035] The raw materials for preparing the probiotic repair promoting agent include the following components in parts by weight: 2.2 parts of probiotic agents, 7 parts of Stipa isophylla, 11 parts of liverwort, 9 parts of fescue, 2.6 parts of coralline lactone, 2.3 parts of hydroxytyrosol, 3.2 parts of N-acetyl-L-hydroxyproline, 2.8 parts of L-alanyl-L-glutamine, 19 parts of polypropylene imine triamine dendrimer, 16 parts of kaempferol-3-O-rutinoside, 1.3 parts of 4-N,N-dimethylpyridine, 10 parts of polyglutamic acid, and 10 parts of polyvinyl pyrrolidone.

[0036] The raw materials for preparing the wound repair elastic matrix include the following components in parts by weight: 38 parts of polytetramethylene ether glycol, 36 parts of cellulose nanocrystals, 47 parts of waterborne polyurethane, 16 parts of carbodiimide, 2.5 parts of bismorpholinyl diethyl ether, 5 parts of benzoic acid, 10 parts of hydrolyzed sponge microneedles, 27 parts of elastin peptides, and 15 parts of acrylic resin.

[0037] The preparation method of the probiotic-containing repair-promoting agent specifically comprises the following steps: S1. Put Stipa indica, Marchantia polyphylla and Festuca australis into a pulping machine with a power of 2.2 kW, the pulping temperature is 33°C, the pulping time is 38 min, the pulping speed is 19000 r / min, and the pulping is performed. After the pulping, it is filtered with a sterile filter cloth to obtain a nutrient solution; S2, the probiotic agent, the nutrient solution prepared in S1, and the 5% sterile glucose solution were placed in a constant temperature shaking incubator with a power of 2.3 kW, the constant temperature shaking culture temperature was 30°C, the constant temperature shaking culture time was 30 h, the constant temperature shaking culture speed was 220 r / min, and after the constant temperature shaking culture, the probiotic agent was placed in a stirrer with a power of 1.6 kW, the stirring temperature was 28°C, the stirring speed was 310 r / min, the stirring time was 26 min, and the mixture was stirred to obtain a repair-promoting suspension; S3, placing polypropylene imine triamine dendrimer, kaempferol-3-O-rutinoside, 4-N,N-dimethylpyridine and 50% ethanol solution in a reactor with a power of 2.4 kW, the reaction temperature is 65°C, the reaction time is 20 h, the reaction speed is 90 r / min, and after grafting reaction, a composite solution is obtained; S4, putting polyglutamic acid, polyvinyl pyrrolidone and ultrapure water into a constant temperature stirrer with a power of 1.7 kW, the constant temperature stirring temperature is 38°C, the constant temperature stirring speed is 160 r / min, the constant temperature stirring time is 32 h, and the constant temperature stirring is performed to obtain an antioxidant film-forming solution; S5, put the repair-promoting suspension prepared by S2 into the complex solution prepared by S3, with the stirring temperature at 32°C, the stirring time at 17 min, the stirring speed at 360 r / min, and after mixing, put it into a vacuum freeze-nano spray dryer with a power of 2.7 kW, the vacuum degree at -0.05 MPa, the freeze-nano spray drying temperature at -40°C, the freeze-nano spray drying particle size at 100 nm, the freeze-nano spray drying time at 2.8 h, and perform freeze-spray drying to obtain repair-promoting nanoparticles; S6. Use a 1.9 kW sprayer to evenly spray the antioxidant film-forming solution prepared in S4 onto the surface of the pro-repair nanoparticles prepared in S5. Place the solution at 28°C for 1.3 h to obtain a pro-repair agent containing probiotics.

[0038] In S2, the probiotic agent consists of Lactobacillus plantarum, Staphylococcus aureus, and Corynebacterium stagnation, with a weight ratio of 0.9:1.1:1. The Lactobacillus plantarum was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.12934, the Staphylococcus aureus was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.8022, and the Corynebacterium stagnation was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.844. The solid-liquid ratio of the probiotic agent to 5% sterile glucose solution is 1:100 g / mL. In S3, the mass fraction of the polypropyleneimine triamine dendrimer in the 50% ethanol solution is 15%.

[0039] In S4, the mass fraction of the polyglutamic acid in ultrapure water is 3%.

[0040] The preparation method of the wound repair elastic matrix specifically comprises the following steps: L1, polytetramethylene ether glycol, cellulose nanocrystals, anhydrous ethanol, and dimethyl sulfoxide were placed in a reactor with a power of 2.3 kW, the reaction temperature was 60°C, the reaction time was 22 h, and the reaction speed was 270 r / min, and the elastic composite solution was obtained; L2, the elastic composite solution prepared in L1, water-based polyurethane, carbodiimide, bismorpholinyl diethyl ether, ultrapure water, and ethyl acetate were placed in a constant temperature stirrer with a power of 1.8 kW, the constant temperature stirring temperature was 70°C, the constant temperature stirring speed was 700 r / min, the constant temperature stirring time was 7 h, and the constant temperature stirring was carried out to perform a cross-linking reaction to form a composite dressing with elasticity and strong hygroscopicity; L3, put benzoic acid, hydrolyzed sponge microneedles, elastin peptide, acrylic resin, and 50% ethanol solution into a stirrer with a power of 1.6 kW, stirring at a temperature of 70°C, a stirring speed of 260 r / min, and a stirring time of 2.1 h to mix and obtain a repair-promoting composition; L4. Place the repair-promoting composition prepared in L3 into the composite dressing prepared in L2, stir at a temperature of 35°C, for 26 min, at a speed of 400 r / min, stir and mix to allow for full adsorption to obtain an elastic matrix for repairing wounds.

[0041] In L1, the mass fraction of the polytetramethylene ether glycol in anhydrous ethanol is 7%, the mass fraction of the cellulose nanocrystals in dimethyl sulfoxide is 3%, in L2, the mass fraction of the carbodiimide in ethyl acetate is 10%, and the mass fraction of the waterborne polyurethane in ultrapure water is 39%.

[0042] In L3, the solid-liquid ratio of the benzoic acid to the 50% ethanol solution is 1:20 g / mL.

[0043] This embodiment also provides a method for preparing a probiotic skin repair composite dressing, which specifically comprises the following steps: Step 1: Put gentian extract, kava root extract, dihydroavenous alkaloids, and anhydrous ethanol into a 1.7 kW stirrer at a stirring temperature of 34° C., a stirring speed of 320 r / min, and a stirring time of 17 min, and stir and mix to obtain a moisturizing care solution; Step 2: Put the wound repair elastic matrix into the moisturizing care solution prepared in step 1, with a stirring temperature of 28°C, a stirring speed of 400 r / min, and a stirring time of 18 min. After mixing, put it into a UV lamp sterilizer with a power of 1.4 kW, a UV lamp sterilization wavelength of 253.7 nm, a UV lamp sterilization temperature of 28°C, and a UV lamp sterilization time of 40 min. Sterilize with UV lamp to obtain a repair-promoting composite matrix; Step 3: Place the probiotic repair-promoting agent into the repair-promoting composite matrix prepared in step 2, use an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and obtain a probiotic skin repair composite dressing after uniform dispersion.

[0044] In step 1, the mass fraction of the gentian extract in anhydrous ethanol is 4.2%.

[0045] Example 3: This example provides a probiotic skin repair composite dressing and a preparation method thereof. The raw materials for preparing the probiotic skin repair composite dressing specifically include the following components in parts by weight: Contains 37 parts of probiotic repair agents, 112 parts of wound repair elastic matrix, 7 parts of gentian extract, 8 parts of kava root extract, and 4.6 parts of dihydrooat alkaloids.

[0046] The raw materials for preparing the probiotic repair promoting agent include the following components in parts by weight: 2.4 parts of probiotic agents, 9 parts of Stipa isophylla, 12 parts of liverwort, 11 parts of fescue, 2.8 parts of coralline lactone, 2.6 parts of hydroxytyrosol, 3.4 parts of N-acetyl-L-hydroxyproline, 3 parts of L-alanyl-L-glutamine, 21 parts of polypropylene imine triamine dendrimer, 19 parts of kaempferol-3-O-rutinoside, 1.4 parts of 4-N,N-dimethylpyridine, 12 parts of polyglutamic acid, and 11 parts of polyvinyl pyrrolidone.

[0047] The raw materials for preparing the wound repair elastic matrix include the following components in parts by weight: 42 parts of polytetramethylene ether glycol, 45 parts of cellulose nanocrystals, 52 parts of waterborne polyurethane, 18 parts of carbodiimide, 2.7 parts of bismorpholinyl diethyl ether, 6 parts of benzoic acid, 12 parts of hydrolyzed sponge microneedles, 31 parts of elastin peptides, and 17 parts of acrylic resin.

[0048] The preparation method of the probiotic-containing repair-promoting agent specifically comprises the following steps: S1. Put Stipa indica, Marchantia polyphylla and Festuca australis into a pulping machine with a power of 2.4 kW, set the pulping temperature to 36°C, the pulping time to 40 min, and the pulping speed to 20000 r / min for pulping. After the pulping, filter with a sterile filter cloth to obtain a nutrient solution. S2, the probiotic agent, the nutrient solution prepared in S1, and the 5% sterile glucose solution were placed in a constant temperature shaking incubator with a power of 2.5 kW, the constant temperature shaking culture temperature was 30°C, the constant temperature shaking culture time was 32 h, the constant temperature shaking culture speed was 230 r / min, and after the constant temperature shaking culture, the probiotic agent was placed in a stirrer with a power of 1.8 kW, the stirring temperature was 33°C, the stirring speed was 320 r / min, the stirring time was 28 min, and the mixture was stirred to obtain a repair-promoting suspension; S3, placing polypropylene imine triamine dendrimer, kaempferol-3-O-rutinoside, 4-N,N-dimethylpyridine and 50% ethanol solution in a reactor with a power of 2.6 kW, the reaction temperature is 70°C, the reaction time is 24 h, the reaction speed is 110 r / min, and after grafting reaction, a composite solution is obtained; S4, putting polyglutamic acid, polyvinyl pyrrolidone and ultrapure water into a constant temperature stirrer with a power of 1.9 kW, the constant temperature stirring temperature is 40°C, the constant temperature stirring speed is 180 r / min, the constant temperature stirring time is 36 h, and the constant temperature stirring is performed to obtain an antioxidant film-forming solution; S5, put the repair-promoting suspension prepared by S2 into the complex solution prepared by S3, with the stirring temperature at 35°C, the stirring time at 20 min, and the stirring speed at 380 r / min. After mixing, put it into a vacuum freeze-nano spray dryer with a power of 2.8 kW, the vacuum degree at -0.05 MPa, the freeze-nano spray drying temperature at -40°C, the freeze-nano spray drying particle size at 100 nm, and the freeze-nano spray drying time at 3 h, and perform freeze-spray drying to obtain repair-promoting nanoparticles; S6. Use a 2.3 kW sprayer to evenly spray the antioxidant film-forming solution prepared in S4 onto the surface of the pro-repair nanoparticles prepared in S5. Place the solution at 30°C for 1.4 h to obtain a pro-repair agent containing probiotics.

[0049] In S2, the probiotic agent consists of Lactobacillus plantarum, Staphylococcus aureus, and Corynebacterium stagnation, with a weight ratio of 1.1:1.2:1. The Lactobacillus plantarum was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.12934, the Staphylococcus aureus was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.8022, and the Corynebacterium stagnation was purchased from the China General Microbiological Culture Collection Administration Center with a preservation number of CGMCC1.844. The solid-liquid ratio of the probiotic agent to 5% sterile glucose solution is 1:100 g / mL. In S3, the mass fraction of the polypropyleneimine triamine dendrimer in the 50% ethanol solution is 17%.

[0050] In S4, the mass fraction of the polyglutamic acid in ultrapure water is 4%.

[0051] The preparation method of the wound repair elastic matrix specifically comprises the following steps: L1, polytetramethylene ether glycol, cellulose nanocrystals, anhydrous ethanol, and dimethyl sulfoxide were placed in a reactor with a power of 2.6 kW, the reaction temperature was 60°C, the reaction time was 24 h, and the reaction speed was 280 r / min, and the elastic composite solution was obtained; L2, the elastic composite solution prepared in L1, water-based polyurethane, carbodiimide, bismorpholinyl diethyl ether, ultrapure water, and ethyl acetate were placed in a constant temperature stirrer with a power of 1.9 kW, the constant temperature stirring temperature was 80°C, the constant temperature stirring speed was 800 r / min, the constant temperature stirring time was 8 h, and the constant temperature stirring was carried out to perform a cross-linking reaction to form a composite dressing with elasticity and strong hygroscopicity; L3, put benzoic acid, hydrolyzed sponge microneedles, elastin peptide, acrylic resin, and 50% ethanol solution into a stirrer with a power of 1.8 kW, stirring at a temperature of 80°C, a stirring speed of 280 r / min, and a stirring time of 2.4 h to mix and obtain a repair-promoting composition; L4. Place the repair-promoting composition prepared in L3 into the composite dressing prepared in L2, stir at a temperature of 36°C, for 28 min, at a speed of 500 r / min, stir and mix to allow for full adsorption to obtain an elastic matrix for repairing wounds.

[0052] In L1, the mass fraction of the polytetramethylene ether glycol in anhydrous ethanol is 8%, the mass fraction of the cellulose nanocrystals in dimethyl sulfoxide is 3%, in L2, the mass fraction of the carbodiimide in ethyl acetate is 10%, and the mass fraction of the waterborne polyurethane in ultrapure water is 42%.

[0053] In L3, the solid-liquid ratio of the benzoic acid to the 50% ethanol solution is 1:20 g / mL.

[0054] This embodiment also provides a method for preparing a probiotic skin repair composite dressing, which specifically comprises the following steps: Step 1, put gentian extract, kava root extract, dihydroavenous alkaloids, and anhydrous ethanol into a 1.8 kW stirrer, stir at a temperature of 36° C., stir at a speed of 340 r / min, stir for 18 min, and stir to obtain a moisturizing care solution; Step 2: Put the wound repair elastic matrix into the moisturizing care solution prepared in step 1, with a stirring temperature of 33°C, a stirring speed of 400 r / min, and a stirring time of 20 min. After mixing, put it into a 1.4 kW ultraviolet lamp sterilizer, the ultraviolet lamp sterilization wavelength is 253.7 nm, the ultraviolet lamp sterilization temperature is 28°C, and the ultraviolet lamp sterilization time is 40 min. Sterilize it with the ultraviolet lamp to obtain a repair-promoting composite matrix; Step 3: Place the probiotic repair-promoting agent into the repair-promoting composite matrix prepared in step 2, use an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min, and obtain a probiotic skin repair composite dressing after uniform dispersion.

[0055] In step 1, the mass fraction of the gentian extract in anhydrous ethanol is 4.8%.

[0056] Comparative Example 1: This comparative example provides a skin repair composite dressing and a preparation method thereof, which is different from Example 1 only in that the added probiotic-containing pro-repair agent does not contain a pro-repair suspension, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0057] Comparative Example 2: This comparative example provides a skin repair composite dressing and a preparation method thereof. The only difference from Example 1 is that the added wound repair elastic matrix does not contain the composite dressing, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0058] Experimental Example 1: Test for measuring the repair ability of the skin.

[0059] The test steps for determining the skin repair ability of the probiotic skin repair composite dressing prepared in Experimental Examples 1-3 of the present invention are as follows: (1) 60 mice, each weighing 30 g (purchased from Chengdu Dashuo Animal Co., Ltd.), were randomly divided into 6 groups of 10 mice each. The mice were housed in separate cages, which were kept clean and dry at a temperature of 24°C and a humidity of 50%, with free access to food and water. (2) The hair on the left side of the back spine of each mouse was shaved to expose a 2 cm × 2 cm bare skin area, and an anesthetic was injected. A circular wound with a diameter of 1 cm and a depth of 2 mm was scratched in the bare skin area. The probiotic skin repair composite dressing prepared in Example 1-3, the skin repair composite dressing prepared in Comparative Example 1-2, and the commercially available skin wound repair dressing (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.) in the control group were used respectively. The circular wound was applied once a day with each dressing until the wound was completely covered for repair. The healing conditions of the wounds of the mice in Example 1-3, Comparative Example 1-2, and the control group were observed on the 3rd, 5th, 7th, and 10th days. The wound area was measured, and the wound healing rate was calculated according to the formula: wound healing rate (%) = (wound area of ​​the mouse before treatment - unhealed wound area of ​​the mouse after treatment) × 100% / wound area of ​​the mouse before treatment; (3) After the wounds of the mice in Example 1-3, Comparative Example 1-2, and Control groups healed, the skin repair of the mice was observed. The absence of obvious color difference between the wound and the skin of other parts of the body and the height of the wound from the wound surface to the normal skin surface by less than 1 mm were used as observation indicators for complete skin repair. The skin repair grade was set, with skin repair days ≤ 14 days as grade I; 14 days ≤ skin repair days ≤ 21 days as grade II; > 21 days as grade III. The specific test results are shown in Table 1.

[0060] Table 1. Mouse wound skin repair grade determination table

[0061] Result analysis: Figure 1 This is an electron microscope image of the internal structure of the composite dressing of the present invention. Figure 2 This is a graph showing the wound healing rate of mice described in Experimental Example 1 of the present invention. Figure 2 As shown in Table 1, the probiotic skin repair composite dressings prepared in Examples 1-3 were used to apply and cover the wounds of the mice in groups 1-3 of Examples. The wound healing rates on the 10th day were all 100%, and the skin repair grades were all grade I. The skin repair composite dressing prepared in Comparative Example 1 was used to apply and cover the wounds of the mice in Group 1 of Comparative Example 1, and the wound healing rates on the 10th day were 64.3%. The skin repair composite dressing prepared in Comparative Example 2 was applied and covered the wounds of the mice in Group 2 of Comparative Example, and the wound healing rates on the 10th day were 82.7%. The commercially available skin wound repair dressing in the control group was applied and covered the wounds of the mice in the control group, and the wound healing rate on the 10th day was 52.8%, indicating that the probiotic skin repair composite dressing prepared in the present invention is easily absorbed by skin cells and has high efficiency in skin healing and repair.

[0062] Experimental Example 2: Tensile strength and water absorption test.

[0063] The test steps for measuring the tensile strength and water absorption of the probiotic skin repair composite dressing prepared in Experimental Examples 1-3 of the present invention are as follows: (1) Weigh 8 g of the probiotic skin repair composite dressing prepared in Examples 1-3 as Example 1-3 group, weigh 8 g of the skin repair composite dressing prepared in Comparative Examples 1-2 as Comparative Example 1-2 group, and weigh 8 g of commercially available skin wound repair dressing (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.) as CK control group. Weigh 8 parallels for each group; (2) The dressings of Examples 1-3, Comparative Examples 1-2 and the control group were molded into dumbbell-shaped tensile specimens, and tested according to the method described in the standard GB / T 1040.3-2006, specifically using a 2 kN fixture, an inlet force of 0.1 N, and a tensile rate of 30 mm / min. Eight specimens were stretched in each group to obtain an average value, and the tensile strength of the probiotic skin repair composite dressings of Examples 1-3, the skin repair composite dressings of Comparative Examples 1-2 and the commercially available skin wound repair dressing of the control group before recovery was obtained; (3) Soaking the probiotic skin repair composite dressings of Example 1-3, Comparative Example 1-2 and the commercially available skin wound repair dressing of the control group, the tensile strength of which has been measured in step (2), in a sufficient amount of ultrapure water until the volume no longer changes, and then placing them in a drying oven for drying until the probiotic skin repair composite dressings of Example 1-3, Comparative Example 1-2 and the commercially available skin wound repair dressing of the control group recover to their original weight, thereby obtaining the recycled probiotic skin repair composite dressings of Example 1-3, Comparative Example 1-2 and the commercially available skin wound repair dressing of the control group; (4) The recovered probiotic skin repair composite dressings of Example 1-3, the skin repair composite dressings of Comparative Example 1-2, and the commercially available skin wound repair dressing of the control group were molded into dumbbell-shaped tensile test specimens, and tested according to the method described in the standard "GB / T1040.3-2006", specifically using a 2 kN fixture, an inlet force of 0.1 N, and a tensile rate of 30 mm / min. Eight specimens were stretched in each group to obtain an average value, and the tensile strength of the recovered probiotic skin repair composite dressings of Example 1-3, the skin repair composite dressings of Comparative Example 1-2, and the commercially available skin wound repair dressing of the control group was obtained; (5) Weigh 8 g of the probiotic skin repair composite dressing prepared in Examples 1-3 as Example 1-3 group, weigh 8 g of the skin repair composite dressing prepared in Comparative Examples 1-2 as Comparative Example 1-2 group, and weigh 8 g of commercially available skin wound repair dressing (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.) as CK control group. Weigh 8 replicates for each group; (6) The water absorption rate of the hydrogel was tested according to GB / T8939-2008, and then calculated according to the following formula: water absorption rate (g / g) = (mass after saturated water absorption - mass before absorption) / mass before absorption.

[0064] Result analysis: Figure 3 This is a tensile strength result diagram of the skin repair dressing described in Experimental Example 2 of the present invention. As shown in the figure, the probiotic skin repair composite dressings prepared in Examples 1-3 are molded into dumbbell-shaped tensile specimens and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths are 8.2 MPa, 8.7 MPa, and 9.5 MPa, respectively. After recovery, the probiotic skin repair composite dressings prepared in Examples 1-3 are molded into dumbbell-shaped tensile specimens and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths are 8.1 MPa, 8.5 MPa, and 9.3 MPa, respectively. The skin repair composite dressings prepared in Comparative Examples 1-2 are molded into dumbbell-shaped tensile specimens and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths are 8.0 MPa, 4.2 MPa, after recovery, the skin repair composite dressings prepared in comparative examples 1-2 were molded into dumbbell-shaped tensile specimens, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths were 7.9 MPa and 3.9 MPa, respectively. The commercially available skin wound repair dressings in the control group were molded into dumbbell-shaped tensile specimens, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strength was 2.6 MPa. After recovery, the commercially available skin wound repair dressings in the control group were molded into dumbbell-shaped tensile specimens, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strength was 1.5 MPa; Figure 4 This is a result graph of the water absorption rate of the skin repair dressing described in Experimental Example 2 of the present invention. As shown in the figure, the probiotic skin repair composite dressings prepared in Examples 1-3 were tested for the water absorption rate of the hydrogel according to GB / T8939-2008, and the water absorption rates were 691 g / g, 697 g / g, and 709 g / g, respectively. The skin repair composite dressings prepared in Comparative Examples 1-2 were tested for the water absorption rate of the hydrogel according to GB / T8939-2008, and the water absorption rates were 685 g / g and 427 g / g, respectively. The commercially available skin wound repair dressing in the control group was tested for the water absorption rate of the hydrogel according to GB / T8939-2008, and the water absorption rate was 386 g / g, indicating that the probiotic skin repair composite dressing prepared by the present invention has strong toughness, is not easy to break, has excellent mechanical properties, is highly hygroscopic, and does not need to be replaced multiple times during use.

[0065] Experimental Example 3: Long-term anti-inflammatory and skin irritation test.

[0066] The test steps for the long-term anti-inflammatory and skin irritation determination of the probiotic skin repair composite dressing prepared in Experimental Examples 1-3 of the present invention are as follows: (1) Take mouse macrophages in good growth condition and adjust the cell density to 1×10 6 / mL was inoculated into a 24-well culture plate, and after the cells grew to 80% confluence, LPS (final concentration of 1 mg / mL) was added to stimulate mouse macrophages for 12 h to induce an inflammatory response; (2) Add 50 mg of the probiotic skin repair composite dressings of Example 1-3, the skin repair composite dressings of Comparative Example 1-2, and the commercially available skin wound repair dressing of the control group (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.) to the 24-well culture plate in step (1), and continue culturing for 24 h. Collect the cell culture supernatant of each group, and use TNF-α kit (purchased from Wuhan Aidikang Biotechnology Co., Ltd., catalog number AD40104) and IL-6 Elisa kit (purchased from Wuhan Aidikang Biotechnology Co., Ltd., catalog number AD40133) to detect the content of cytokines in the supernatant; (3) Sixty healthy white male guinea pigs weighing 700 g were randomly divided into six groups of 10; (4) Conventional hair removal was performed on both sides of the back of white guinea pigs, with a hair removal range of 3 cm × 3 cm. After 24 hours of hair removal, the local skin was observed to be intact and without damage. The experiment used the left and right sides of the same guinea pig for comparison. The left side of the back of the guinea pigs in the Example 1-3 groups was smeared with the probiotic skin repair composite dressing prepared in Example 1-3, and the left side of the back of the guinea pigs in the Comparative Example 1-2 groups was smeared with the skin repair composite dressing prepared in Comparative Example 1-2 groups. The left side of the back of the guinea pigs in the control group was smeared with a commercially available skin wound repair dressing (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.). The guinea pigs in each group were smeared once a day for 10 hours each time. After continuous use for 10 days, the treated area was cleaned with warm water and observed; (5) Observe with the naked eye 24, 48, and 72 hours after cleaning with warm water to see if there is any erythema or edema on the treated area on the left side of the guinea pig's back. Calculate the average score of each group of guinea pigs at each observation time point according to Table 2. Evaluate the intensity of stimulation according to Table 3 and record the results in Table 4.

[0067] Table 2. Skin irritation response scoring criteria

[0068] Table 3. Evaluation criteria for skin irritation intensity

[0069] Table 4. Skin irritation reaction results

[0070] Result analysis: Figure 5 This is a result graph of the cytokine content of the macrophage supernatant described in Experimental Example 3 of the present invention. As shown in the figure, lipopolysaccharide was used to stimulate mouse macrophages for 12 h, and after inducing an inflammatory response, the probiotic skin repair composite dressings prepared in Examples 1-3 were added respectively, and the co-culture was continued for 24 h. The TNF-α content in the supernatant of the macrophages in Groups 1-3 of Examples was 3612.3 pg / mL, 3524.1 pg / mL, and 3414.4 pg / mL, respectively, and the IL-6 content was 552.0 pg / mL, 541.3 pg / mL, and 531.2 pg / mL, respectively. Lipopolysaccharide was used to stimulate mouse macrophages for 12 h, and after inducing an inflammatory response, the skin repair composite dressings prepared in Comparative Examples 1-2 were added respectively, and the co-culture was continued for 24 h. The TNF-α content in the supernatant of the macrophages in Groups 1-2 of Comparative Examples was 4125.7 pg / mL, 3712.3 pg / mL, respectively, and the IL-6 content was 597.2 pg / mL, 561.3 pg / mL, lipopolysaccharide was used to stimulate mouse macrophages for 12 h, and after inducing inflammatory response, the commercially available skin wound repair dressing of the control group was added and co-cultured for 24 h. The TNF-α content in the macrophage supernatant of the control group was 4563.3 pg / mL, and the IL-6 content was 655.7 pg / mL. The physiological structure of guinea pig skin is similar to that of human skin. The probiotic skin repair composite dressings prepared in Example 1-3 and the skin repair composite dressings prepared in Comparative Example 1-2 were applied to the back of guinea pigs for a long time, and no erythema or edema appeared on the back of the white guinea pigs. The commercially available skin wound repair dressing of the control group was applied to the back of guinea pigs for a long time, and mild irritation appeared on the back of the white guinea pigs. This shows that the probiotic skin repair composite dressing prepared by the present invention will not cause inflammation if used for a long time, and it is comfortable and non-irritating to use.

[0071] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0072] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A probiotic skin repair composite dressing, characterized in that: The probiotic skin repair composite dressing specifically comprises the following components in parts by weight: Contains 28-37 parts of probiotic repair agent, 105-112 parts of wound repair elastic matrix, 5-7 parts of gentian extract, 4-8 parts of kava root extract, and 2.9-4.6 parts of dihydroavenate alkaloids; The raw materials for preparing the probiotic repair promoting agent include the following components in parts by weight: 1.5-2.4 parts of probiotic agent, 6-9 parts of Stipa isophylla, 10-12 parts of liverwort, 7-11 parts of fescue, 2.1-2.8 parts of coral lactone, 1.9-2.6 parts of hydroxytyrosol, 2.1-3.4 parts of N-acetyl-L-hydroxyproline, 2.5-3 parts of L-alanyl-L-glutamine, 17-21 parts of polypropylene imine triamine dendrimer, 14-19 parts of kaempferol-3-O-rutinoside, 1.2-1.4 parts of 4-N,N-dimethylpyridine, 8-12 parts of polyglutamic acid, and 9-11 parts of polyvinylpyrrolidone; The raw materials for preparing the wound repair elastic matrix include the following components in parts by weight: 35-42 parts of polytetramethylene ether glycol, 33-45 parts of cellulose nanocrystals, 40-52 parts of waterborne polyurethane, 14-18 parts of carbodiimide, 2.4-2.7 parts of bismorpholinyl diethyl ether, 4-6 parts of benzoic acid, 8-12 parts of hydrolyzed sponge microneedles, 23-31 parts of elastin peptides, and 14-17 parts of acrylic resin.

2. The probiotic skin repair composite dressing according to claim 1, characterized in that: The preparation method of the probiotic-containing repair-promoting agent specifically comprises the following steps: S1. Grind Stipa indica, Marchantia polyphylla and Festuca arundinacea and filter using a sterile filter cloth to obtain a nutrient solution; S2, mixing the probiotic agent with the nutrient solution prepared in S1 and 5% sterile glucose solution, and mixing with coral phytolide, hydroxytyrosol, N-acetyl-L-hydroxyproline, and L-alanyl-L-glutamine to obtain a repair-promoting suspension; S3, mixing polypropylene imine triamine dendrimer, kaempferol-3-O-rutinoside, 4-N,N-dimethylpyridine with 50% ethanol solution, performing grafting reaction, and obtaining a composite solution; S4, mixing polyglutamic acid, polyvinyl pyrrolidone and ultrapure water to obtain an antioxidant film-forming solution; S5, placing the repair-promoting suspension prepared in S2 into the complex solution prepared in S3, mixing well and then freeze-spray drying to obtain repair-promoting nanoparticles; S6. Spray the antioxidant film-forming solution prepared in S4 evenly on the surface of the pro-repair nanoparticles prepared in S5, and place it at a temperature of 25-30°C for 1.2-1.4 h to obtain a pro-repair agent containing probiotics.

3. The probiotic skin repair composite dressing according to claim 2, characterized in that: The preparation method of the wound repair elastic matrix specifically comprises the following steps: L1, mixing polytetramethylene ether glycol, cellulose nanocrystals, anhydrous ethanol and dimethyl sulfoxide for modification reaction to obtain an elastic composite solution; L2, mixing the elastic composite solution prepared in L1 with waterborne polyurethane, carbodiimide, bismorpholinyl diethyl ether, ultrapure water, and ethyl acetate, stirring to carry out a cross-linking reaction to form a composite dressing; L3. Mix benzoic acid, hydrolyzed sponge microneedles, elastin peptide, acrylic resin and 50% ethanol solution, put them into the composite dressing prepared in L2, stir and adsorb to obtain an elastic matrix for repairing wounds.

4. A method for preparing the probiotic skin repair composite dressing according to any one of claims 1 to 3, characterized in that: The specific steps include: Step 1: After fully dissolving gentian extract, kava root extract, dihydrooat alkaloids, and anhydrous ethanol, the mixture is mixed with a wound repair elastic matrix, and sterilized with an ultraviolet lamp to obtain a repair-promoting composite matrix; Step 2: Add the probiotic repair-promoting agent into the repair-promoting composite matrix prepared in step 1, and evenly disperse it to obtain a probiotic skin repair composite dressing.

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