A probiotic skin repair composite dressing and its preparation method

Abstract

The present invention belongs to the field of preparation of skin repair dressings, and particularly relates to a probiotic skin repair composite dressing and a preparation method thereof. A nutrient solution obtained by grinding Stipa aliena, Marchantia polymorpha, and Festuca ovina is used to culture a probiotic agent, and the two cooperate to enhance the skin repair effect. A graft reaction is carried out between polypropylene imine triamine dendrimer and kaempferol-3-O-rutinoside to load a repair-promoting suspension, and an antioxidant film-forming solution is sprayed on the surface of the repair-promoting nanoparticles, so that the obtained probiotic repair-promoting agent can efficiently and continuously play a repair-promoting role; after polytetramethylene ether glycol and cellulose nanocrystals are combined, a cross-linking reaction is carried out with waterborne polyurethane, and the obtained composite dressing adsorbs a repair-promoting composition to prepare a wound repair elastic matrix with strong flexibility; a repair-promoting composite matrix is mixed with the probiotic repair-promoting agent, and the prepared probiotic skin repair composite dressing can efficiently repair the skin, has strong flexibility, and provides a comfortable use feeling.

Description

Technical Field

[0001] The present invention belongs to the field of preparation of skin repair dressings, and specifically refers to a probiotic skin repair composite dressing and a preparation method thereof. Background Art

[0002] A skin repair dressing is a medical dressing used for treating and repairing skin injuries. It mainly helps the skin recover to health by providing functions such as protection, promoting healing, and reducing infection. Skin repair dressings can be divided into various types, such as transparent dressings, hydrogel dressings, silicone dressings, liquid dressings, etc. These dressings are usually composed of medical glue, medical gauze, bioactive ingredients, or polymer materials.

[0003] The skin repair dressing can cover the surface of the injured skin to form a physical barrier, prevent external bacteria, dust, and other pollutants from entering the wound, reduce the risk of infection, and can also prevent friction and external irritation, relieve pain and discomfort. The skin repair dressing accelerates wound healing by keeping the wound moist and providing a good environment for cell growth and regeneration. Some dressings also contain drug components, such as antibacterial drugs, growth factors, or cytokines, which can further promote wound healing.

[0004] At present, the existing skin repair dressing preparation technologies have the following problems: First, poor absorbency. Traditional dressings such as gauze and petrolatum gauze have limited absorption capacity, making it difficult to meet the needs of long-term repair. The dressing change frequency is high, it is easy to adhere to the wound surface, and secondary mechanical damage may be caused during replacement, prolonging the healing time. Second, poor flexibility. The existing skin repair dressings have poor mechanical properties and are easy to be brittle, making it difficult to adapt to a dynamic environment. Third, the existing skin repair dressings are prone to bacterial growth, have poor biocompatibility, are difficult to effectively degrade in the body, and long-term use will cause inflammatory reactions. Summary of the Invention

[0005] In view of the above situation, in order to overcome the defects of the existing technology, the present invention provides a probiotic skin repair composite dressing and a preparation method thereof. To solve the problems of poor absorbency, long skin healing time, poor flexibility, and inflammatory reactions caused by long-term use of existing skin repair dressings, the present invention uses a wound repair elastic matrix, a moisturizing care solution, and a probiotic-promoting repair agent mixture. The prepared probiotic skin repair composite dressing has strong absorbency, can efficiently promote skin healing, has excellent flexibility, has no irritation during long-term use, and will not cause inflammatory reactions.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows. The present invention provides a probiotic skin repair composite dressing. The preparation raw materials of the probiotic skin repair composite dressing specifically include the following components in parts by weight:

[0007] It contains 28 - 37 parts of probiotic promoting repair agent, 105 - 112 parts of wound - repairing elastic matrix, 5 - 7 parts of gentian extract, 4 - 8 parts of kava root extract, and 2.9 - 4.6 parts of dihydro - avenanthramide.

[0008] Preferably, the raw materials for preparing the probiotic promoting repair agent include the following components in parts by weight: 1.5 - 2.4 parts of probiotic agent, 6 - 9 parts of Stipa aliena, 10 - 12 parts of Marchantia polymorpha, 7 - 11 parts of Festuca ovina, 2.1 - 2.8 parts of phellopterin, 1.9 - 2.6 parts of hydroxytyrosol, 2.1 - 3.4 parts of N - acetyl - L - hydroxyproline, 2.5 - 3 parts of L - alanyl - L - glutamine, 17 - 21 parts of polypropyleneimine triamine dendrimer, 14 - 19 parts of kaempferol - 3 - O - rutinoside, 1.2 - 1.4 parts of 4 - N,N - dimethylpyridine, 8 - 12 parts of polyglutamic acid, and 9 - 11 parts of polyvinylpyrrolidone.

[0009] Preferably, the raw materials for preparing the wound - repairing elastic matrix include the following components in parts by weight: 35 - 42 parts of polytetramethylene ether glycol, 33 - 45 parts of cellulose nanocrystals, 40 - 52 parts of aqueous polyurethane, 14 - 18 parts of carbodiimide, 2.4 - 2.7 parts of bis - morpholinodiethyl ether, 4 - 6 parts of benzoic acid, 8 - 12 parts of hydrolyzed sponge microneedles, 23 - 31 parts of elastin peptides, and 14 - 17 parts of acrylic resin.

[0010] Preferably, the preparation method of the probiotic promoting repair agent specifically includes the following steps:

[0011] S1. Put Stipa aliena, Marchantia polymorpha, and Festuca ovina into a grinder with a power of 1.8 - 2.4 kW, the grinding temperature is 30 - 36 °C, the grinding time is 35 - 40 min, and the grinding speed is 18000 - 20000 r / min for grinding. After grinding, filter with a sterile filter cloth to obtain a nutrient solution;

[0012] S2. Put the probiotic agent, the nutrient solution prepared in S1, and a 5% sterile glucose solution into a constant - temperature shaking incubator with a power of 2.2 - 2.5 kW. The constant - temperature shaking culture temperature is 30 °C, the constant - temperature shaking culture time is 28 - 32 h, and the constant - temperature shaking culture speed is 210 - 230 r / min. After constant - temperature shaking culture, put it together with phellopterin, hydroxytyrosol, N - acetyl - L - hydroxyproline, and L - alanyl - L - glutamine into a stirrer with a power of 1.5 - 1.8 kW. The stirring temperature is 25 - 33 °C, the stirring speed is 300 - 320 r / min, and the stirring time is 23 - 28 min for stirring and mixing to obtain a promoting repair suspension;

[0013] S3. Put polypropyleneimine triamine dendrimer, kaempferol-3-O-rutinoside, 4-N,N-dimethylpyridine and 50% ethanol solution into a reaction kettle with a power of 2.2 - 2.6 kW, with a reaction temperature of 60 - 70 °C, a reaction duration of 18 - 24 h, and a reaction rotation speed of 80 - 110 r / min. After carrying out the grafting reaction, a complex solution is obtained;

[0014] S4. Put polyglutamic acid, polyvinylpyrrolidone and ultrapure water into a thermostatic stirrer with a power of 1.6 - 1.9 kW, with a thermostatic stirring temperature of 35 - 40 °C, a thermostatic stirring rotation speed of 140 - 180 r / min, and a thermostatic stirring duration of 24 - 36 h. Carry out thermostatic stirring to obtain an antioxidant film-forming solution;

[0015] S5. Put the pro-repair suspension prepared in S2 into the complex solution prepared in S3, with a stirring temperature of 28 - 35 °C, a stirring duration of 15 - 20 min, and a stirring rotation speed of 350 - 380 r / min. After mixing evenly, put it into a vacuum freeze nano spray dryer with a power of 2.3 - 2.8 kW, with a vacuum degree of -0.05 MPa, a freeze nano spray drying temperature of -40 °C, a freeze nano spray drying particle size of 100 nm, and a freeze nano spray drying duration of 2.5 - 3 h. Carry out freeze spray drying to obtain pro-repair nanoparticles;

[0016] S6. Use a spraying machine with a power of 1.6 - 2.3 kW to spray the antioxidant film-forming solution prepared in S4 evenly on the surface of the pro-repair nanoparticles prepared in S5, with a spraying duration of 13 - 18 min and a spraying temperature of 25 - 30 °C. Place it for 1.2 - 1.4 h under the condition of a temperature of 25 - 30 °C to obtain a probiotic pro-repair agent.

[0017] Furthermore, in S2, the probiotic agent is composed of Lactobacillus plantarum, Staphylococcus xylosus, and Corynebacterium stationis, and the weight ratio mixture is 0.8 - 1.1:1.1 - 1.2:1. The Lactobacillus plantarum is purchased from the China General Microbiological Culture Collection Center, with a preservation number of CGMCC1.12934. The Staphylococcus xylosus is purchased from the China General Microbiological Culture Collection Center, with a preservation number of CGMCC1.8022. The Corynebacterium stationis is purchased from the China General Microbiological Culture Collection Center, with a preservation number of CGMCC1.844. The material ratio of the probiotic agent to the 5% sterile glucose solution is 1:100 g / mL. In S3, the mass fraction of the polypropyleneimine triamine dendrimer in the 50% ethanol solution is 13 - 17%.

[0018] Furthermore, in S4, the mass fraction of the polyglutamic acid in the ultrapure water is 2 - 4%.

[0019] Preferably, the preparation method of the wound repair elastic matrix specifically includes the following steps:

[0020] L1. Put polytetramethylene ether glycol, cellulose nanocrystals, absolute ethanol, and dimethyl sulfoxide into a reaction kettle with a power of 2.2 - 2.6 kW, a reaction temperature of 60 °C, a reaction duration of 18 - 24 h, and a reaction rotation speed of 260 - 280 r / min for modification to obtain an elastic composite solution;

[0021] L2. Put the elastic composite solution prepared in L1, waterborne polyurethane, carbodiimide, bis(morpholino)diethyl ether, ultrapure water, and ethyl acetate into a constant temperature stirrer with a power of 1.6 - 1.9 kW, a constant temperature stirring temperature of 60 - 80 °C, a constant temperature stirring rotation speed of 600 - 800 r / min, and a constant temperature stirring duration of 6 - 8 h for cross-linking reaction to form an elastic and highly hygroscopic composite dressing;

[0022] L3. Put benzoic acid, hydrolyzed sponge microneedles, elastin peptides, acrylic resin, and 50% ethanol solution into a stirrer with a power of 1.5 - 1.8 kW, a stirring temperature of 60 - 80 °C, a stirring rotation speed of 200 - 280 r / min, and a stirring duration of 1.8 - 2.4 h for mixing to obtain a repair promoting composition;

[0023] L4. Put the repair promoting composition prepared in L3 into the composite dressing prepared in L2, with a stirring temperature of 30 - 36 °C, a stirring duration of 20 - 28 min, and a stirring rotation speed of 300 - 500 r / min for stirring and mixing to fully adsorb and obtain the wound repair elastic matrix.

[0024] Furthermore, in L1, the mass fraction of polytetramethylene ether glycol in absolute ethanol is 6 - 8%, the mass fraction of cellulose nanocrystals in dimethyl sulfoxide is 3%, in L2, the mass fraction of carbodiimide in ethyl acetate is 10%, and the mass fraction of waterborne polyurethane in ultrapure water is 35 - 42%.

[0025] Furthermore, in L3, the material ratio of benzoic acid to 50% ethanol solution is 1:20 g / mL.

[0026] The present invention also proposes a preparation method of a probiotic skin repair composite dressing, which specifically includes the following steps:

[0027] Step 1: Put gentiana scabra bunge extract, piper methysticum root extract, dihydrooat alkaloids, and absolute ethanol into a stirrer with a power of 1.5 - 1.8 kW, at a stirring temperature of 33 - 36 °C, a stirring speed of 310 - 340 r / min, and a stirring duration of 15 - 18 min, and stir and mix to obtain a moisturizing care solution;

[0028] Step 2: Put the wound - repairing elastic matrix into the moisturizing care solution prepared in Step 1, at a stirring temperature of 25 - 33 °C, a stirring speed of 300 - 400 r / min, and a stirring duration of 15 - 20 min. After mixing evenly, put it into an ultraviolet lamp sterilizer with a power of 1.4 kW. The wavelength of the ultraviolet lamp for sterilization is 253.7 nm, the temperature for ultraviolet lamp sterilization is 28 °C, and the duration of ultraviolet lamp sterilization is 40 min. Sterilize with the ultraviolet lamp to obtain a repair - promoting composite matrix;

[0029] Step 3: Put the probiotic repair - promoting agent into the repair - promoting composite matrix prepared in Step 2, and use an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20000 r / min, a stirring temperature of 25 °C, and a stirring duration of 10 min. After uniform dispersion, a probiotic skin - repair composite dressing is obtained.

[0030] Preferably, in Step 1, the mass fraction of the gentiana scabra bunge extract in absolute ethanol is 3.2 - 4.8%.

[0031] The beneficial effects of the present invention are as follows:

[0032] The present invention uses a probiotic agent containing Lactobacillus plantarum, Staphylococcus xylosus, and Corynebacterium stationis, which can regulate the balance of the skin microbiota, reduce the release of inflammatory factors, promote the synthesis of collagen, supplement the ceramide level in the skin, thereby maintaining the homeostasis of the skin barrier, and accelerating skin re-epithelialization and promoting wound healing by stimulating the proliferation and migration of keratinocytes. The juices obtained by grinding Stipa aliena, Marchantia polymorpha, and Festuca ovina contain rich natural organic substances, which can provide carbon and nitrogen sources for bacteria and support their growth and metabolism. The nutrient solution also contains antioxidant and anti-inflammatory components, which synergistically enhance the skin repair effect with the probiotic agent. Phellopterin, hydroxytyrosol, N-acetyl-L-hydroxyproline, and L-alanyl-L-glutamine can promote skin nerve regeneration, reduce the damage of oxidative stress to skin cells, moisturize and enhance the skin barrier function, and promote the proliferation and differentiation of skin cells. Under the catalytic action of 4-N,N-dimethylpyridine, the amino group in the polypropyleneimine triamine dendrimer reacts with the hydroxyl group in kaempferol-3-O-rutinoside to form an amide bond, thereby achieving grafting. The obtained composite solution has good biocompatibility, can efficiently load the repair suspension, and achieve the sustained release of the active ingredients. After mixing polyglutamic acid and polyvinylpyrrolidone, an antioxidant film-forming solution is formed through hydrogen bond interaction and sprayed on the surface of the repair nanoparticles. The obtained probiotic-containing repair agent is easy to be taken up by cells and can efficiently and continuously play a repair-promoting role. A non-covalent bond can be formed between the ether oxygen group of polytetramethylene ether glycol and the hydroxyl group on the surface of cellulose nanocrystals. The flexibility of polytetramethylene ether glycol is combined with cellulose nanocrystals, making the elastic composite have good mechanical properties and avoiding brittle fracture. Waterborne polyurethane has strong hygroscopicity. Under the action of carbodiimide and bis(morpholino)diethyl ether, a crosslinking reaction occurs between waterborne polyurethane, polytetramethylene ether glycol, and cellulose nanocrystals to obtain a composite dressing. Benzoic acid has antibacterial effects. Hydrolyzed sponge microneedles can form a large number of microchannels on the skin surface, significantly increasing skin permeability. Elastin peptides are combined with acrylic resin to provide a good adhesion and growth environment for skin cells, thereby accelerating wound healing and avoiding the appearance of scars. The composite dressing fully adsorbs the repair composition to obtain a wound-repairing elastic matrix, which does not need to be replaced multiple times and can quickly promote the absorption of skin cells for the active components. The moisturizing care solution plays anti-inflammatory, sedative, and soothing roles. By mixing the wound-repairing elastic matrix and the probiotic-containing repair agent, the prepared probiotic skin repair composite dressing is easy to be absorbed by skin cells, has high efficiency in skin healing and repair, strong flexibility, strong hygroscopicity, and will not cause inflammation after long-term use, and has a comfortable use feeling. Brief Description of the Drawings

[0033] To more clearly illustrate the technical solutions in the present invention or the prior art, the following will be described in a clear and understandable manner in combination with the accompanying drawings. Obviously, the accompanying drawings in the following description are only those of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0034] Figure 1 SEM image of the internal structure of the composite dressing described in the present invention;

[0035] Figure 2 Graph of the wound healing rate of mice in Experimental Example 1 of the present invention;

[0036] Figure 3 Graph of the tensile strength of the skin repair dressing in Experimental Example 2 of the present invention;

[0037] Figure 4 Graph of the water absorption ratio of the skin repair dressing in Experimental Example 2 of the present invention;

[0038] Figure 5 Graph of the cytokine content in the macrophage supernatant in Experimental Example 3 of the present invention. Detailed implementation manners

[0039] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in combination with the accompanying drawings in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of them; based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0040] Unless otherwise defined, all professional and scientific terms used herein have the same meaning as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to the described content can be applied to the present invention. The preferred implementation methods and materials described herein are only for illustration purposes and do not limit the content of this application.

[0041] The experimental methods in the following embodiments are all conventional methods unless otherwise specified; the test materials and test strains used in the following embodiments are all obtained from commercial channels unless otherwise specified.

[0042] Example 1: This example provides a probiotic skin repair composite dressing and its preparation method. The raw materials for preparing the probiotic skin repair composite dressing specifically include the following components in parts by weight:

[0043] 28 parts of probiotic promoting repair agent, 105 parts of elastic matrix for wound repair, 5 parts of gentian extract, 4 parts of kava root extract, and 2.9 parts of dihydrooat alkaloid.

[0044] The preparation raw materials of the probiotic promoting repair agent include the following components in parts by weight: 1.5 parts of probiotic agent, 6 parts of Stipa aliena, 10 parts of Marchantia polymorpha, 7 parts of Festuca ovina, 2.1 parts of goshonolide, 1.9 parts of hydroxytyrosol, 2.1 parts of N-acetyl-L-hydroxyproline, 2.5 parts of L-alanyl-L-glutamine, 17 parts of polypropyleneimine triamine dendrimer, 14 parts of kaempferol-3-O-rutinoside, 1.2 parts of 4-N,N-dimethylpyridine, 8 parts of polyglutamic acid, and 9 parts of polyvinylpyrrolidone.

[0045] The preparation raw materials of the wound repair elastic matrix include the following components in parts by weight: 35 parts of polytetramethylene ether glycol, 33 parts of cellulose nanocrystals, 40 parts of aqueous polyurethane, 14 parts of carbodiimide, 2.4 parts of bis(morpholino)diethyl ether, 4 parts of benzoic acid, 8 parts of hydrolyzed sponge microneedles, 23 parts of elastin peptides, and 14 parts of acrylic resin.

[0046] The preparation method of the probiotic promoting repair agent specifically includes the following steps:

[0047] S1. Put Stipa aliena, Marchantia polymorpha, and Festuca ovina into a pulping machine with a power of 1.8 kW, a pulping temperature of 30 °C, a pulping duration of 35 min, and a pulping speed of 18,000 r / min for pulping. After pulping, filter with a sterile filter cloth to obtain a nutrient solution.

[0048] S2. Put the probiotic agent, the nutrient solution prepared in S1, and a 5% sterile glucose solution into a constant temperature shaking incubator with a power of 2.2 kW. The constant temperature shaking culture temperature is 30 °C, the constant temperature shaking culture duration is 28 h, and the constant temperature shaking culture speed is 210 r / min. After constant temperature shaking culture, put it into a stirrer with a power of 1.5 kW together with goshonolide, hydroxytyrosol, N-acetyl-L-hydroxyproline, and L-alanyl-L-glutamine. The stirring temperature is 25 °C, the stirring speed is 300 r / min, and the stirring duration is 23 min for stirring and mixing to obtain a promoting repair suspension.

[0049] S3. Put polypropyleneimine triamine dendrimer, kaempferol-3-O-rutinoside, 4-N,N-dimethylpyridine, and a 50% ethanol solution into a reaction kettle with a power of 2.2 kW. The reaction temperature is 60 °C, the reaction duration is 18 h, and the reaction speed is 80 r / min. After grafting reaction, a composite solution is obtained.

[0050] S4. Put polyglutamic acid, polyvinylpyrrolidone, and ultrapure water into a constant temperature stirrer with a power of 1.6 kW. The constant temperature stirring temperature is 35 °C, the constant temperature stirring speed is 140 r / min, and the constant temperature stirring duration is 24 h for constant temperature stirring to obtain an antioxidant film-forming solution.

[0051] S5. Put the repair-promoting suspension prepared in S2 into the complex solution prepared in S3. The stirring temperature is 28 °C, the stirring duration is 15 min, and the stirring speed is 350 r / min. After mixing evenly, put it into a vacuum freeze-drying nano spray dryer with a power of 2.3 kW. The vacuum degree is -0.05 MPa, the freeze-drying nano spray drying temperature is -40 °C, the particle size of freeze-drying nano spray drying is 100 nm, and the freeze-drying nano spray drying duration is 2.5 h. Carry out freeze spray drying to obtain repair-promoting nanoparticles;

[0052] S6. Use a spraying machine with a power of 1.6 kW to spray the antioxidant film-forming solution prepared in S4. The spraying duration is 13 min, and the spraying temperature is 25 °C. Spray it evenly on the surface of the repair-promoting nanoparticles prepared in S5 and place it at 25 °C for 1.2 h to obtain a probiotic repair-promoting agent.

[0053] In S2, the probiotic agent is composed of Lactobacillus plantarum, Staphylococcus xylosus, and Corynebacterium stationis, and the weight ratio is 0.8:1.1:1. The Lactobacillus plantarum is purchased from the China General Microbiological Culture Collection Center, and the preservation number is CGMCC1.12934. The Staphylococcus xylosus is purchased from the China General Microbiological Culture Collection Center, and the preservation number is CGMCC1.8022. The Corynebacterium stationis is purchased from the China General Microbiological Culture Collection Center, and the preservation number is CGMCC1.844. The material ratio of the probiotic agent to the 5% sterile glucose solution is 1:100 g / mL. In S3, the mass fraction of the polypropyleneimine triamine dendrimer in the 50% ethanol solution is 13%.

[0054] In S4, the mass fraction of the polyglutamic acid in the ultrapure water is 2%.

[0055] The preparation method of the wound repair elastic matrix specifically includes the following steps:

[0056] L1. Put polytetramethylene ether glycol, cellulose nanocrystals, anhydrous ethanol, and dimethyl sulfoxide into a reaction kettle with a power of 2.2 kW. The reaction temperature is 60 °C, the reaction duration is 18 h, and the reaction speed is 260 r / min. Carry out modification to obtain an elastic composite solution;

[0057] L2. Put the elastic composite solution prepared in L1, waterborne polyurethane, carbodiimide, bis(morpholino)diethyl ether, ultrapure water, and ethyl acetate into a constant temperature stirrer with a power of 1.6 kW. The constant temperature stirring temperature is 60 °C, the constant temperature stirring speed is 600 r / min, and the constant temperature stirring duration is 6 h. Carry out constant temperature stirring to carry out a cross-linking reaction to form an elastic and highly hygroscopic composite dressing;

[0058] L3. Put benzoic acid, hydrolyzed sponge microneedles, elastin peptides, acrylic resin, and a 50% ethanol solution into a stirrer with a power of 1.5 kW, stir at a temperature of 60 °C, a stirring speed of 200 r / min, and a stirring duration of 1.8 h to mix and obtain a repair-promoting composition;

[0059] L4. Put the repair-promoting composition prepared in L3 into the composite dressing prepared in L2, stir at a temperature of 30 °C, a stirring duration of 20 min, and a stirring speed of 300 r / min, stir and mix, and fully adsorb to obtain a wound-repairing elastic matrix.

[0060] In L1, the mass fraction of polytetramethylene ether glycol in absolute ethanol is 6%, and the mass fraction of cellulose nanocrystals in dimethyl sulfoxide is 3%. In L2, the mass fraction of carbodiimide in ethyl acetate is 10%, and the mass fraction of aqueous polyurethane in ultrapure water is 35%.

[0061] In L3, the material ratio of benzoic acid to the 50% ethanol solution is 1:20 g / mL.

[0062] This embodiment also provides a preparation method of a probiotic skin repair composite dressing, which specifically includes the following steps:

[0063] Step 1. Put gentian extract, kava root extract, dihydrooat alkaloids, and absolute ethanol into a stirrer with a power of 1.5 kW, stir at a temperature of 33 °C, a stirring speed of 310 r / min, and a stirring duration of 15 min, stir and mix to obtain a moisturizing care solution;

[0064] Step 2. Put the wound-repairing elastic matrix into the moisturizing care solution prepared in Step 1, stir at a temperature of 25 °C, a stirring speed of 300 r / min, and a stirring duration of 15 min. After mixing evenly, put it into an ultraviolet lamp sterilizer with a power of 1.4 kW, the ultraviolet lamp sterilization wavelength is 253.7 nm, the ultraviolet lamp sterilization temperature is 28 °C, and the ultraviolet lamp sterilization duration is 40 min. Sterilize with an ultraviolet lamp to obtain a repair-promoting composite matrix;

[0065] Step 3. Put the probiotic repair-promoting agent into the repair-promoting composite matrix prepared in Step 2, use an ultrasonic stirrer with a power of 1.8 kW, the ultrasonic frequency is 15 kHz, the stirring speed is 20000 r / min, the stirring temperature is 25 °C, and the stirring duration is 10 min. After uniform dispersion, obtain a probiotic skin repair composite dressing.

[0066] In Step 1, the mass fraction of gentian extract in absolute ethanol is 3.2%.

[0067] Example 2: This example provides a probiotic skin repair composite dressing and its preparation method. The raw materials for preparing the probiotic skin repair composite dressing specifically include the following components in parts by weight:

[0068] 32 parts of probiotic repair promoter, 109 parts of wound repair elastic matrix, 6 parts of gentian extract, 6 parts of kava root extract, and 3.4 parts of dihydrooat alkaloid.

[0069] The raw materials for preparing the probiotic repair promoter include the following components in parts by weight: 2.2 parts of probiotic agent, 7 parts of Stipa aliena, 11 parts of Marchantia polymorpha, 9 parts of Festuca ovina, 2.6 parts of phellopterin, 2.3 parts of hydroxytyrosol, 3.2 parts of N-acetyl-L-hydroxyproline, 2.8 parts of L-alanyl-L-glutamine, 19 parts of polypropyleneimine triamine dendrimer, 16 parts of kaempferol-3-O-rutinoside, 1.3 parts of 4-N,N-dimethylpyridine, 10 parts of polyglutamic acid, and 10 parts of polyvinylpyrrolidone.

[0070] The raw materials for preparing the wound repair elastic matrix include the following components in parts by weight: 38 parts of polytetramethylene ether glycol, 36 parts of cellulose nanocrystals, 47 parts of waterborne polyurethane, 16 parts of carbodiimide, 2.5 parts of bis(morpholinyl)diethyl ether, 5 parts of benzoic acid, 10 parts of hydrolyzed sponge microneedles, 27 parts of elastin peptides, and 15 parts of acrylic resin.

[0071] The preparation method of the probiotic repair promoter specifically includes the following steps:

[0072] S1. Put Stipa aliena, Marchantia polymorpha, and Festuca ovina into a grinder with a power of 2.2 kW, the grinding temperature is 33 °C, the grinding time is 38 min, and the grinding speed is 19000 r / min for grinding. After grinding, filter with a sterile filter cloth to obtain a nutrient solution;

[0073] S2. Put the probiotic agent, the nutrient solution prepared in S1, and a 5% sterile glucose solution into a constant temperature shaking incubator with a power of 2.3 kW. The constant temperature shaking culture temperature is 30 °C, the constant temperature shaking culture time is 30 h, and the constant temperature shaking culture speed is 220 r / min. After constant temperature shaking culture, put it into a stirrer with a power of 1.6 kW together with phellopterin, hydroxytyrosol, N-acetyl-L-hydroxyproline, and L-alanyl-L-glutamine. The stirring temperature is 28 °C, the stirring speed is 310 r / min, and the stirring time is 26 min for stirring and mixing to obtain a repair promoting suspension;

[0074] S3. Put polypropyleneimine triamine dendrimer, kaempferol-3-O-rutinoside, 4-N,N-dimethylpyridine and 50% ethanol solution into a reaction kettle with a power of 2.4 kW, a reaction temperature of 65 °C, a reaction duration of 20 h, and a reaction rotation speed of 90 r / min. After grafting reaction, a complex solution is obtained;

[0075] S4. Put polyglutamic acid, polyvinylpyrrolidone and ultrapure water into a thermostatic stirrer with a power of 1.7 kW, a thermostatic stirring temperature of 38 °C, a thermostatic stirring rotation speed of 160 r / min, and a thermostatic stirring duration of 32 h. Stir thermostatically to obtain an antioxidant film-forming solution;

[0076] S5. Put the promoting repair suspension prepared in S2 into the complex solution prepared in S3, with a stirring temperature of 32 °C, a stirring duration of 17 min, and a stirring rotation speed of 360 r / min. After mixing evenly, put it into a vacuum freeze nano spray dryer with a power of 2.7 kW, a vacuum degree of -0.05 MPa, a freeze nano spray drying temperature of -40 °C, a freeze nano spray drying particle size of 100 nm, and a freeze nano spray drying duration of 2.8 h. Carry out freeze spray drying to obtain promoting repair nanoparticles;

[0077] S6. Use a spraying machine with a power of 1.9 kW to spray the antioxidant film-forming solution prepared in S4 evenly on the surface of the promoting repair nanoparticles prepared in S5 for 16 min at a spraying temperature of 28 °C. Place it at a temperature of 28 °C for 1.3 h to obtain a probiotic promoting repair agent.

[0078] In S2, the probiotic agent is composed of Lactobacillus plantarum, Staphylococcus xylosus, and Corynebacterium stationis, and the weight ratio is 0.9:1.1:1. The Lactobacillus plantarum is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC1.12934. The Staphylococcus xylosus is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC1.8022. The Corynebacterium stationis is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC1.844. The material ratio of the probiotic agent to 5% sterile glucose solution is 1:100 g / mL. In S3, the mass fraction of the polypropyleneimine triamine dendrimer in 50% ethanol solution is 15%.

[0079] In S4, the mass fraction of the polyglutamic acid in ultrapure water is 3%.

[0080] The preparation method of the wound repair elastic matrix specifically includes the following steps:

[0081] L1. Put polytetramethylene ether glycol, cellulose nanocrystals, absolute ethanol, and dimethyl sulfoxide into a reaction kettle with a power of 2.3 kW. The reaction temperature is 60 °C, the reaction duration is 22 h, and the reaction rotation speed is 270 r / min for modification to obtain an elastic composite solution;

[0082] L2. Put the elastic composite solution prepared in L1, waterborne polyurethane, carbodiimide, bis(morpholino)diethyl ether, ultrapure water, and ethyl acetate into a constant-temperature stirrer with a power of 1.8 kW. The constant-temperature stirring temperature is 70 °C, the constant-temperature stirring rotation speed is 700 r / min, and the constant-temperature stirring duration is 7 h for constant-temperature stirring to carry out a cross-linking reaction to form a composite dressing with elasticity and strong hygroscopicity;

[0083] L3. Put benzoic acid, hydrolyzed sponge microneedles, elastin peptides, acrylic resin, and 50% ethanol solution into a stirrer with a power of 1.6 kW. The stirring temperature is 70 °C, the stirring rotation speed is 260 r / min, and the stirring duration is 2.1 h for mixing to obtain a repair-promoting composition;

[0084] L4. Put the repair-promoting composition prepared in L3 into the composite dressing prepared in L2. The stirring temperature is 35 °C, the stirring duration is 26 min, and the stirring rotation speed is 400 r / min for stirring and mixing and sufficient adsorption to obtain a wound-repairing elastic matrix.

[0085] In L1, the mass fraction of polytetramethylene ether glycol in absolute ethanol is 7%, and the mass fraction of cellulose nanocrystals in dimethyl sulfoxide is 3%. In L2, the mass fraction of carbodiimide in ethyl acetate is 10%, and the mass fraction of waterborne polyurethane in ultrapure water is 39%.

[0086] In L3, the feed ratio of benzoic acid to 50% ethanol solution is 1:20 g / mL.

[0087] This embodiment also provides a preparation method of a probiotic skin repair composite dressing, which specifically includes the following steps:

[0088] Step 1. Put gentian extract, kava root extract, dihydrooat alkaloid, and absolute ethanol into a stirrer with a power of 1.7 kW. The stirring temperature is 34 °C, the stirring rotation speed is 320 r / min, and the stirring duration is 17 min for stirring and mixing to obtain a moisturizing care solution;

[0089] Step 2: Put the wound repair elastic matrix into the moisturizing care solution prepared in Step 1. The stirring temperature is 28°C, the stirring speed is 400 r / min, and the stirring duration is 18 min. After mixing evenly, put it into an ultraviolet sterilizer with a power of 1.4 kW. The ultraviolet sterilization wavelength is 253.7 nm, the ultraviolet sterilization temperature is 28°C, and the ultraviolet sterilization duration is 40 min. Sterilize with ultraviolet light to obtain a repair-promoting composite matrix;

[0090] Step 3: Put the probiotic repair-promoting agent into the repair-promoting composite matrix prepared in Step 2. Use an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring duration of 10 min. After uniform dispersion, a probiotic skin repair composite dressing is obtained.

[0091] In Step 1, the mass fraction of gentian extract in absolute ethanol is 4.2%.

[0092] Example 3: This example provides a probiotic skin repair composite dressing and a preparation method thereof. The preparation raw materials of the probiotic skin repair composite dressing specifically include the following components in parts by weight:

[0093] 37 parts of probiotic repair-promoting agent, 112 parts of wound repair elastic matrix, 7 parts of gentian extract, 8 parts of kava root extract, 4.6 parts of dihydrooat alkaloid.

[0094] The preparation raw materials of the probiotic repair-promoting agent include the following components in parts by weight: 2.4 parts of probiotic agent, 9 parts of Stipa aliena, 12 parts of Marchantia polymorpha, 11 parts of Festuca ovina, 2.8 parts of phellopterin, 2.6 parts of hydroxytyrosol, 3.4 parts of N-acetyl-L-hydroxyproline, 3 parts of L-alanyl-L-glutamine, 21 parts of polypropyleneimine triamine dendrimer, 19 parts of kaempferol-3-O-rutinoside, 1.4 parts of 4-N,N-dimethylpyridine, 12 parts of polyglutamic acid, 11 parts of polyvinylpyrrolidone.

[0095] The preparation raw materials of the wound repair elastic matrix include the following components in parts by weight: 42 parts of polytetramethylene ether glycol, 45 parts of cellulose nanocrystals, 52 parts of aqueous polyurethane, 18 parts of carbodiimide, 2.7 parts of bis(morpholinyl)diethyl ether, 6 parts of benzoic acid, 12 parts of hydrolyzed sponge microneedles, 31 parts of elastin peptide, 17 parts of acrylic resin.

[0096] The preparation method of the probiotic repair-promoting agent specifically includes the following steps:

[0097] S1. Put Stipa aliena, Marchantia polymorpha, and Festuca ovina into a pulper with a power of 2.4 kW. The pulping temperature is 36°C, the pulping duration is 40 min, and the pulping speed is 20,000 r / min. Conduct pulping, and after pulping, filter it with a sterile filter cloth to obtain a nutrient solution;

[0098] S2. Put the probiotic agent, the nutrient solution prepared in S1, and a 5% sterile glucose solution into a thermostatic shaking incubator with a power of 2.5 kW. The thermostatic shaking culture temperature is 30°C, the thermostatic shaking culture duration is 32 h, and the thermostatic shaking culture speed is 230 r / min. After thermostatic shaking culture, put it together with glehnolide, hydroxytyrosol, N-acetyl-L-hydroxyproline, and L-alanyl-L-glutamine into a stirrer with a power of 1.8 kW. The stirring temperature is 33°C, the stirring speed is 320 r / min, and the stirring duration is 28 min. Stir and mix to obtain a repair-promoting suspension;

[0099] S3. Put polypropyleneimine triamine dendrimer, kaempferol-3-O-rutinoside, 4-N,N-dimethylpyridine, and a 50% ethanol solution into a reaction kettle with a power of 2.6 kW. The reaction temperature is 70°C, the reaction duration is 24 h, and the reaction speed is 110 r / min. After carrying out a grafting reaction, obtain a complex solution;

[0100] S4. Put polyglutamic acid, polyvinylpyrrolidone, and ultrapure water into a thermostatic stirrer with a power of 1.9 kW. The thermostatic stirring temperature is 40°C, the thermostatic stirring speed is 180 r / min, and the thermostatic stirring duration is 36 h. Conduct thermostatic stirring to obtain an antioxidant film-forming solution;

[0101] S5. Put the repair-promoting suspension prepared in S2 into the complex solution prepared in S3. The stirring temperature is 35°C, the stirring duration is 20 min, and the stirring speed is 380 r / min. After mixing evenly, put it into a vacuum freeze nano spray dryer with a power of 2.8 kW. The vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, and the freeze nano spray drying duration is 3 h. Conduct freeze spray drying to obtain repair-promoting nanoparticles;

[0102] S6. Use a sprayer with a power of 2.3 kW to spray the antioxidant film-forming solution prepared in S4. The spraying duration is 18 min, and the spraying temperature is 30°C. Spray it evenly on the surface of the repair-promoting nanoparticles prepared in S5, and place it at a temperature of 30°C for 1.4 h to obtain a probiotic-containing repair promoter.

[0103] In S2, the probiotic agent is composed of Lactobacillus plantarum, Staphylococcus xylosus, and Corynebacterium stationis, and the mixing ratio by weight is 1.1:1.2:1. The Lactobacillus plantarum is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC1.12934. The Staphylococcus xylosus is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC1.8022. The Corynebacterium stationis is purchased from the China General Microbiological Culture Collection Center, with the preservation number CGMCC1.844. The material ratio of the probiotic agent to the 5% sterile glucose solution is 1:100 g / mL. In S3, the mass fraction of the polypropyleneimine triamine dendrimer in the 50% ethanol solution is 17%.

[0104] In S4, the mass fraction of the polyglutamic acid in ultrapure water is 4%.

[0105] The preparation method of the wound repair elastic matrix specifically includes the following steps:

[0106] L1. Put polytetramethylene ether glycol, cellulose nanocrystals, absolute ethanol, and dimethyl sulfoxide into a reaction kettle with a power of 2.6 kW, a reaction temperature of 60 °C, a reaction duration of 24 h, and a reaction rotation speed of 280 r / min for modification to obtain an elastic composite solution;

[0107] L2. Put the elastic composite solution prepared in L1, waterborne polyurethane, carbodiimide, bis(morpholino)diethyl ether, ultrapure water, and ethyl acetate into a constant temperature stirrer with a power of 1.9 kW, a constant temperature stirring temperature of 80 °C, a constant temperature stirring rotation speed of 800 r / min, and a constant temperature stirring duration of 8 h for cross-linking reaction to form an elastic and highly hygroscopic composite dressing;

[0108] L3. Put benzoic acid, hydrolyzed sponge microneedles, elastin peptides, acrylic resin, and 50% ethanol solution into a stirrer with a power of 1.8 kW, a stirring temperature of 80 °C, a stirring rotation speed of 280 r / min, and a stirring duration of 2.4 h for mixing to obtain a repair-promoting composition;

[0109] L4. Put the repair-promoting composition prepared in L3 into the composite dressing prepared in L2, with a stirring temperature of 36 °C, a stirring duration of 28 min, and a stirring rotation speed of 500 r / min for stirring and mixing to fully adsorb and obtain the wound repair elastic matrix.

[0110] In L1, the mass fraction of polytetramethylene ether glycol in absolute ethanol is 8%, and the mass fraction of cellulose nanocrystals in dimethyl sulfoxide is 3%. In L2, the mass fraction of carbodiimide in ethyl acetate is 10%, and the mass fraction of aqueous polyurethane in ultrapure water is 42%.

[0111] In L3, the feed ratio of benzoic acid to 50% ethanol solution is 1:20 g / mL.

[0112] This example also provides a preparation method of a probiotic skin repair composite dressing, which specifically includes the following steps:

[0113] Step 1: Put gentian extract, kava root extract, dihydrooat alkaloid, and absolute ethanol into a stirrer with a power of 1.8 kW, stir at a temperature of 36°C, a stirring speed of 340 r / min, and a stirring duration of 18 min, and stir and mix to obtain a moisturizing care solution;

[0114] Step 2: Put the wound repair elastic matrix into the moisturizing care solution prepared in Step 1, stir at a temperature of 33°C, a stirring speed of 400 r / min, and a stirring duration of 20 min. After mixing evenly, put it into an ultraviolet lamp sterilizer with a power of 1.4 kW. The wavelength of the ultraviolet lamp for sterilization is 253.7 nm, the temperature of the ultraviolet lamp for sterilization is 28°C, and the duration of ultraviolet lamp sterilization is 40 min. Sterilize with the ultraviolet lamp to obtain a repair-promoting composite matrix;

[0115] Step 3: Put the probiotic repair-promoting agent into the repair-promoting composite matrix prepared in Step 2, use an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20000 r / min, a stirring temperature of 25°C, and a stirring duration of 10 min. After uniform dispersion, a probiotic skin repair composite dressing is obtained.

[0116] In Step 1, the mass fraction of gentian extract in absolute ethanol is 4.8%.

[0117] Comparative Example 1: This comparative example provides a skin repair composite dressing and its preparation method. The difference from Example 1 is only that the added probiotic repair-promoting agent does not contain a repair-promoting suspension, and the other components, component contents, and method steps are the same as those in Example 1.

[0118] Comparative Example 2: This comparative example provides a skin repair composite dressing and its preparation method. The difference from Example 1 is only that the added wound repair elastic matrix does not contain a composite dressing, and the other components, component contents, and method steps are the same as those in Example 1.

[0119] Experimental Example 1: Determination test for the repair ability of the skin.

[0120] The test steps for measuring the skin repair ability of the probiotic skin repair composite dressing prepared in Experimental Examples 1-3 of the present invention are as follows:

[0121] (1) Select 60 mice, each with a body weight of 30 g (purchased from Chengdu Dashuo Animal Co., Ltd.). Divide them into 6 groups randomly with 10 mice in each group. Keep the mice in separate cages, maintain the cleanliness and dryness in the cages, and feed them at a temperature of 24°C and a humidity of 50% with free access to food and water.

[0122] (2) Shave the hair on the left side of the spine on the back of each mouse to expose a skin-exposed area of 2 cm × 2 cm. Inject an anesthetic and make a circular wound with a diameter of 1 cm and a depth of 2 mm in the skin-exposed area. Apply the probiotic skin repair composite dressings prepared in Examples 1-3, the skin repair composite dressings prepared in Comparative Examples 1-2, and the commercially available skin wound repair dressing (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.) to the circular wounds once a day for each group until the wounds are completely covered for repair. Observe the healing conditions of the wounds of the mice in Examples 1-3, Comparative Examples 1-2, and the control group on the 3rd day, 5th day, 7th day, and 10th day. Measure the wound area and calculate the wound healing rate according to the formula: Wound healing rate (%) = (Wound area before treatment of the mouse - Unhealed wound area after treatment of the mouse) × 100% / Wound area before treatment of the mouse.

[0123] (3) After the wounds of the mice in Examples 1-3, Comparative Examples 1-2, and the control group have healed, observe the skin repair conditions of the mice. Take the condition that there is no obvious color difference between the wound area and the skin of the rest of the body and the height above the normal skin surface is less than 1 mm as the observation index for complete skin repair. Set the skin repair grades: Skin repair days ≤ 14 d is Grade I; 14 d ≤ Skin repair days ≤ 21 d is Grade II; > 21 d is Grade III. The specific test results are shown in Table 1.

[0124] Table 1. Determination table of skin repair grades for mouse wounds

[0125]

[0126] Result analysis:

[0127] Figure 1 This is the electron micrograph of the internal structure of the composite dressing described in the present invention. Figure 2 This is the result graph of the wound healing rate of the mice in Experimental Example 1 of the present invention, as Figure 2As shown in Table 1, the probiotic skin repair composite dressings prepared in Examples 1-3 were used to smear and cover the wounds of the mice in Groups 1-3 of Examples. The wound healing rates on the 10th day were all 100%, and the skin repair grades were all Grade I. The skin repair composite dressing prepared in Comparative Example 1 was used to smear and cover the wounds of the mice in Comparative Example 1. The wound healing rate on the 10th day was 64.3%. The skin repair composite dressing prepared in Comparative Example 2 was used to smear and cover the wounds of the mice in Comparative Example 2. The wound healing rate on the 10th day was 82.7%. The commercially available skin wound repair dressing in the control group was used to smear and cover the wounds of the mice in the control group. The wound healing rate on the 10th day was 52.8%. It shows that the probiotic skin repair composite dressing prepared by the present invention is easy to be absorbed by skin cells and has high efficiency in skin healing and repair.

[0128] Experimental Example 2: Tensile strength and water absorption measurement test.

[0129] The steps for measuring the tensile strength and water absorption of the probiotic skin repair composite dressings prepared in Experimental Examples 1-3 of the present invention are as follows:

[0130] (1) Weigh 8 g of the probiotic skin repair composite dressings prepared in Examples 1-3 as the groups of Examples 1-3, weigh 8 g of the skin repair composite dressings prepared in Comparative Examples 1-2 as the groups of Comparative Examples 1-2, and weigh 8 g of the commercially available skin wound repair dressing (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.) as the CK control group. Weigh 8 parallels for each group.

[0131] (2) Mold the dressings of the above groups of Examples 1-3, groups of Comparative Examples 1-2 and the control group into dumbbell-shaped tensile test specimens, and test them according to the method described in the standard "GB / T 1040.3-2006". Specifically, select a 2 kN fixture, the inlet force is 0.1 N, and the tensile rate is 30 mm / min. Tensile 8 specimen strips for each group to take the average value to obtain the tensile strength of the probiotic skin repair composite dressings in the groups of Examples 1-3, the skin repair composite dressings in the groups of Comparative Examples 1-2 and the commercially available skin wound repair dressing in the control group before recovery.

[0132] (3) Immerse the probiotic skin repair composite dressings in the groups of Examples 1-3, the skin repair composite dressings in the groups of Comparative Examples 1-2 and the commercially available skin wound repair dressing in the control group, which have been measured for tensile strength in step (2), in sufficient ultrapure water until the volume no longer changes, and then put them into a drying oven for drying until the probiotic skin repair composite dressings in the groups of Examples 1-3, the skin repair composite dressings in the groups of Comparative Examples 1-2 and the commercially available skin wound repair dressing in the control group return to their original weights, to obtain the probiotic skin repair composite dressings in the groups of Examples 1-3, the skin repair composite dressings in the groups of Comparative Examples 1-2 and the commercially available skin wound repair dressing in the control group after recovery.

[0133] (4)The probiotic skin repair composite dressings in Example Groups 1-3, the skin repair composite dressings in Comparative Example Groups 1-2, and the commercially available skin wound repair dressings in the control group after recycling were molded into dumbbell-shaped tensile test specimens. The tests were carried out according to the method described in the standard "GB / T1040.3-2006". Specifically, a 2 kN fixture was selected, the inlet force was 0.1 N, and the tensile rate was 30 mm / min. Eight specimen strips were stretched for each group to obtain the average value, and the tensile strength of the probiotic skin repair composite dressings in Example Groups 1-3, the skin repair composite dressings in Comparative Example Groups 1-2, and the commercially available skin wound repair dressings in the control group after recycling was obtained.

[0134] (5)Weigh 8 g of the probiotic skin repair composite dressings prepared in Examples 1-3 as Example Groups 1-3, weigh 8 g of the skin repair composite dressings prepared in Comparative Examples 1-2 as Comparative Example Groups 1-2, and weigh 8 g of the commercially available skin wound repair dressings (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.) as the CK control group. Eight parallels were weighed for each group.

[0135] (6)The water absorption ratio of the hydrogel was tested according to GB / T8939-2008, and then calculated according to the following formula: Water absorption ratio (g / g) = (mass after saturated water absorption - mass before absorption) / mass before absorption.

[0136] Result analysis:

[0137] Figure 3This is the graph of the tensile strength results of the skin repair dressing described in Experimental Example 2 of the present invention. As shown in the figure, the probiotic skin repair composite dressings prepared in Examples 1-3 were molded into dumbbell-shaped tensile test specimens, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths were 8.2 MPa, 8.7 MPa, and 9.5 MPa respectively. After recovery, the probiotic skin repair composite dressings prepared in Examples 1-3 were molded into dumbbell-shaped tensile test specimens, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths were 8.1 MPa, 8.5 MPa, and 9.3 MPa respectively. The skin repair composite dressings prepared in Comparative Examples 1-2 were molded into dumbbell-shaped tensile test specimens, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths were 8.0 MPa and 4.2 MPa respectively. After recovery, the skin repair composite dressings prepared in Comparative Examples 1-2 were molded into dumbbell-shaped tensile test specimens, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strengths were 7.9 MPa and 3.9 MPa respectively. The commercially available skin wound repair dressing in the control group was molded into a dumbbell-shaped tensile test specimen, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strength was 2.6 MPa. After recovery, the commercially available skin wound repair dressing in the control group was molded into a dumbbell-shaped tensile test specimen, and tested according to the method described in the standard "GB / T 1040.3-2006". The tensile strength was 1.5 MPa; Figure 4 This is the graph of the water absorption ratio results of the skin repair dressing described in Experimental Example 2 of the present invention. As shown in the figure, the water absorption ratios of the probiotic skin repair composite dressings prepared in Examples 1-3 were tested according to GB / T8939-2008, and the water absorption ratios were 691 g / g, 697 g / g, and 709 g / g respectively. The water absorption ratios of the skin repair composite dressings prepared in Comparative Examples 1-2 were tested according to GB / T8939-2008, and the water absorption ratios were 685 g / g and 427 g / g respectively. The water absorption ratio of the commercially available skin wound repair dressing in the control group was tested according to GB / T8939-2008, and the water absorption ratio was 386 g / g. It shows that the probiotic skin repair composite dressing prepared by the present invention has strong toughness, is not easy to break, has excellent mechanical properties, strong hygroscopicity, and does not need to be replaced many times during use.

[0138] Experimental Example 3: Long-term anti-inflammatory and skin irritation determination test.

[0139] The steps of the long-term anti-inflammatory and skin irritation determination test of the probiotic skin repair composite dressings prepared in Experimental Examples 1-3 of the present invention are as follows:

[0140] (1) Take mouse macrophages in good growth state and adjust the cell concentration to 1×10 6 / mL with DMEM medium, inoculate them into a 24-well culture plate. After the cells grow to 80% confluence, add LPS (final concentration 1 mg / mL) to stimulate the mouse macrophages for 12 h to induce an inflammatory response;

[0141] (2) In the 24-well culture plates in step (1), add 50 mg of the probiotic skin repair composite dressings in Example Groups 1-3, the skin repair composite dressings in Comparative Example Groups 1-2, and the commercially available skin wound repair dressing (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.) in the control group, and then continue culturing for 24 h. Collect the cell culture supernatants of each group, and use a TNF-α kit (purchased from Wuhan Aidyant Biotech Co., Ltd., product number AD40104) and an IL-6 Elisa kit (purchased from Wuhan Aidyant Biotech Co., Ltd., product number AD40133) to detect the content of cytokines in the supernatants;

[0142] (3) Select 60 healthy white male guinea pigs weighing 700 g, divide them into 6 groups with 10 in each group randomly;

[0143] (4) Conduct routine hair removal on both sides of the back of the white guinea pigs, with the hair removal area being 3 cm×3 cm. After 24 h of hair removal, observe that the local skin is intact without damage. In the experiment, the left and right sides of the same guinea pig are compared with each other. Apply the probiotic skin repair composite dressings prepared in Examples 1-3 to the left side of the back of the guinea pigs in Example Groups 1-3, apply the skin repair composite dressings prepared in Comparative Example Groups 1-2 to the left side of the back of the guinea pigs in Comparative Example Groups 1-2, and apply the commercially available skin wound repair dressing (purchased from Shanxi Jinbo Biopharmaceutical Co., Ltd.) to the left side of the back of the guinea pigs in the control group. Each group of guinea pigs is applied once a day, each application for 10 h. After continuous use for 10 d, observe the area after cleaning with warm water;

[0144] (5) At 24, 48, and 72 h after cleaning with warm water, observe and record with the naked eye whether there are erythema and edema on the treated area on the left side of the guinea pig's back. Calculate the average score of each group of guinea pigs at each observation time point according to Table 2, and conduct a skin irritation intensity evaluation according to Table 3, and record the results in Table 4.

[0145] Table 2. Skin irritation reaction scoring criteria

[0146]

[0147] Table 3. Skin irritation intensity evaluation criteria

[0148]

[0149] Table 4. Skin irritation reaction result table

[0150]

[0151] Result analysis:

[0152] Figure 5 This is the result graph of the cytokine content in the supernatant of macrophages described in Experimental Example 3 of the present invention. As shown in the figure, after stimulating mouse macrophages with lipopolysaccharide for 12 h to induce an inflammatory response, the probiotic skin repair composite dressings prepared in Examples 1-3 were added respectively, and then co-cultured for another 24 h. The TNF-α contents in the macrophage supernatants of the groups of Examples 1-3 were 3612.3 pg / mL, 3524.1 pg / mL, and 3414.4 pg / mL respectively, and the IL-6 contents were 552.0 pg / mL, 541.3 pg / mL, and 531.2 pg / mL respectively. After stimulating mouse macrophages with lipopolysaccharide for 12 h to induce an inflammatory response, the skin repair composite dressings prepared in Comparative Examples 1-2 were added respectively, and then co-cultured for another 24 h. The TNF-α contents in the macrophage supernatants of the groups of Comparative Examples 1-2 were 4125.7 pg / mL and 3712.3 pg / mL respectively, and the IL-6 contents were 597.2 pg / mL and 561.3 pg / mL respectively. After stimulating mouse macrophages with lipopolysaccharide for 12 h to induce an inflammatory response, the commercially available skin wound repair dressing of the control group was added, and then co-cultured for another 24 h. The TNF-α content in the macrophage supernatant of the control group was 4563.3 pg / mL, and the IL-6 content was 655.7 pg / mL. The skin physiological structure of guinea pigs is similar to that of humans. When the probiotic skin repair composite dressings prepared in the groups of Examples 1-3 and the skin repair composite dressings prepared in the groups of Comparative Examples 1-2 were applied to the backs of guinea pigs for a long time, no erythema or edema appeared on the backs of white guinea pigs. However, when the commercially available skin wound repair dressing of the control group was applied to the backs of guinea pigs for a long time, mild irritation appeared on the backs of white guinea pigs. This shows that the probiotic skin repair composite dressing prepared by the present invention will not cause inflammation after long-term use, has a comfortable feeling in use and no irritation.

[0153] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

[0154] The present invention and its embodiments have been described above. Such description is not restrictive. What is shown in the drawings is only one of the embodiments of the present invention, and the actual application is not limited thereto. Generally speaking, if those of ordinary skill in the art are inspired by it and design similar ways and embodiments without creative efforts without departing from the purpose of the present invention, they should all fall within the protection scope of the present invention.

Claims

1. A probiotic skin repair composite dressing, characterized in that: The probiotic skin repair composite dressing specifically comprises the following components in parts by weight: Contains 28-37 parts of probiotic repair agent, 105-112 parts of wound repair elastic matrix, 5-7 parts of gentian extract, 4-8 parts of kava root extract, and 2.9-4.6 parts of dihydroavenate alkaloids; The preparation method of the probiotic-containing repair-promoting agent specifically comprises the following steps: S1, grinding 6-9 parts by weight of Stipa indica, 10-12 parts by weight of Marchantia polyphylla, and 7-11 parts by weight of Festuca arundinacea, filtering with a sterile filter cloth to obtain a nutrient solution; S2, mixing 1.5-2.4 parts by weight of the probiotic agent with the nutrient solution prepared in S1 and a 5% sterile glucose solution, and mixing with 2.1-2.8 parts of coral lactone, 1.9-2.6 parts of hydroxytyrosol, 2.1-3.4 parts of N-acetyl-L-hydroxyproline, and 2.5-3 parts of L-alanyl-L-glutamine to obtain a repair-promoting suspension; S3, mixing 17-21 parts by weight of polypropyleneimine triamine dendrimer, 14-19 parts by weight of kaempferol-3-O-rutinoside, and 1.2-1.4 parts by weight of 4-N,N-dimethylpyridine with a 50% ethanol solution for grafting reaction to obtain a composite solution; S4, mixing 8-12 parts by weight of polyglutamic acid, 9-11 parts of polyvinyl pyrrolidone, and ultrapure water to obtain an antioxidant film-forming solution; S5, placing the repair-promoting suspension prepared in S2 into the complex solution prepared in S3, mixing well and then freeze-spray drying to obtain repair-promoting nanoparticles; S6, spraying the antioxidant film-forming solution prepared in S4 uniformly on the surface of the repair-promoting nanoparticles prepared in S5, leaving it at a temperature of 25-30° C. for 1.2-1.4 h to obtain a probiotic-containing repair-promoting agent; The preparation method of the wound repair elastic matrix specifically comprises the following steps: L1, mixing 35-42 parts by weight of polytetramethylene ether glycol, 33-45 parts by weight of cellulose nanocrystals, anhydrous ethanol, and dimethyl sulfoxide for modification reaction to obtain an elastic composite solution; L2, mixing the elastic composite solution prepared in L1 with 40-52 parts by weight of aqueous polyurethane, 14-18 parts by weight of carbodiimide, 2.4-2.7 parts by weight of bismorpholinyl diethyl ether, ultrapure water, and ethyl acetate, stirring for cross-linking reaction to form a composite dressing; L3. Mix 4-6 parts by weight of benzoic acid, 8-12 parts by weight of hydrolyzed sponge microneedles, 23-31 parts by weight of elastin peptides, 14-17 parts by weight of acrylic resin, and 50% ethanol solution, put into the composite dressing prepared in L2, stir and adsorb to obtain an elastic matrix for repairing wounds.

2. A method for preparing the probiotic skin repair composite dressing according to claim 1, characterized in that: The specific steps include: Step 1: After fully dissolving gentian extract, kava root extract, dihydrooat alkaloids, and anhydrous ethanol, the mixture is mixed with a wound repair elastic matrix, and sterilized with an ultraviolet lamp to obtain a repair-promoting composite matrix; Step 2: Add the probiotic repair-promoting agent into the repair-promoting composite matrix prepared in step 1, and evenly disperse it to obtain a probiotic skin repair composite dressing.

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