Biological fertilizer with bacteriostatic effect for plant protection

Abstract

The invention relates to the technical field of fertilizers, in particular to a plant protection biological fertilizer with an antibacterial effect, which comprises the following components in parts by weight: 40-60 parts of organic waste, 20-25 parts of animal waste, 6-10 parts of bone meal, 10-16 parts of basic soil and 6-15 parts of a natural bacteriostatic agent, the natural bacteriostatic agent comprises pine needle polysaccharide, folium isatidis extract and polygonum cuspidatum extract. By adding various natural antibacterial components, the biological fertilizer has a long-term antibacterial effect, growth and reproduction of pathogens in soil or the fertilizer are effectively inhibited, and the dosage of chemical pesticides is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, and in particular to a biofertilizer with antibacterial effect for plant protection. Background Art

[0002] Biofertilizer is a type of fertilizer that combines the effects of microbial fertilizer and organic fertilizer. It provides necessary nutrients for crops through the life activities of specific functional microorganisms, while improving soil structure and enhancing crop growth conditions.

[0003] The ingredients of biological fertilizers mainly include organic waste and animal manure, which may contain a large number of human and animal pathogens. These pathogens may continue to survive and multiply after being applied to farmland, and even contaminate the crops planted. Although biological organic fertilizers are designed to reduce or eliminate harmful microorganisms through full decomposition and harmless treatment, they cannot completely eliminate the presence of pathogens. After subsequent trials in the fields, the remaining pathogens will inevitably multiply and erupt again.

[0004] Based on this, the present invention provides a biofertilizer with antibacterial effect for plant protection. By utilizing the natural properties of the antibacterial components added to the fertilizer, it can not only promote plant growth, but also effectively inhibit pathogens in the soil, thereby reducing the occurrence of crop diseases. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a biofertilizer with antibacterial effect for plant protection.

[0006] The technical scheme adopted by the present invention is as follows: a biofertilizer with antibacterial effect for crop protection, comprising the following components in parts by weight: 40-60 parts of organic waste, 20-25 parts of animal feces, 6-10 parts of bone meal, 10-16 parts of basic soil, and 6-15 parts of natural antibacterial agent;

[0007] The natural antibacterial agent comprises pine needle polysaccharide, isatis indigo plant extract and knotweed extract.

[0008] Furthermore, the weight ratio of the pine needle polysaccharide, the isatis indigotica extract and the knotweed extract is 2-3:2-6:2-6.

[0009] Furthermore, a biofertilizer with antibacterial effect for crop protection includes the following components in parts by weight: 50 parts of organic waste, 22.5 parts of animal manure, 8 parts of bone meal, 13 parts of basic soil, 2.5 parts of pine needle polysaccharides, 4 parts of isatis indigotica extract, and 4 parts of knotweed extract.

[0010] Furthermore, the organic waste includes leaves, straw, rice bran, and soybean meal.

[0011] Furthermore, the basic soil includes peat soil, leaf mold, coal gangue, and diatomaceous earth.

[0012] Furthermore, the organic waste includes the following components in parts by weight: 5-10 parts of leaves, 15-20 parts of straws, 5-10 parts of rice bran, and 10-30 parts of soybean meal.

[0013] Furthermore, the basic soil comprises the following components in parts by weight: 2-4 parts of peat soil, 2-3 parts of leaf mold, 3-5 parts of coal gangue, and 3-4 parts of diatomaceous earth.

[0014] Furthermore, the bone meal includes fish bone meal.

[0015] Furthermore, the animal excrement is cow dung.

[0016] The present invention also discloses a method for preparing a biofertilizer with antibacterial effect for plant protection, comprising the following steps:

[0017] Step 1, weighing each component by weight;

[0018] Step 2, adding fish bone meal, organic waste, basic soil and an appropriate amount of water to the cow dung, stirring evenly, fermenting and decomposing, the fermentation temperature is 40-65° C., the fermentation time is 45-60 days, and after the fermentation is completed, spreading and airing to obtain basic bio-fertilizer;

[0019] Step three, adding pine needle polysaccharide, isatis indigo leaf extract and knotweed extract into the basic bio-fertilizer, mixing evenly again, and crushing to obtain bio-fertilizer with antibacterial effect.

[0020] The beneficial effects achieved by the present invention using the above structure are as follows:

[0021] 1. The added pine needle polysaccharide can increase the organic matter content of the soil, help improve the soil structure, has antibacterial activity, inhibits the growth of pathogens in the soil, and reduces the occurrence of plant diseases;

[0022] 2. The added isatis leaf extract contains a variety of bioactive substances, which inhibit bacteria and are beneficial to the development of the root system and the activity of soil microorganisms;

[0023] 3. Knotweed extract may indirectly promote plant growth by improving soil fertility and plant disease resistance;

[0024] 4. The addition of a variety of natural antibacterial ingredients makes the biological fertilizer have a long-lasting antibacterial effect, effectively inhibiting the growth and reproduction of pathogens in the soil or fertilizer, and reducing the use of chemical pesticides. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0026] A biofertilizer with antibacterial effect for plant protection, comprising the following components in parts by weight: organic waste, animal feces, bone meal, basic soil, pine needle polysaccharide, isatis leaf extract, and knotweed extract;

[0027] in:

[0028] Polygonum cuspidatum extract contains a variety of bioactive substances, such as resveratrol and rhein, which have been proven to promote plant growth. Adding Polygonum cuspidatum extract to the soil may indirectly promote plant growth by improving soil fertility and plant disease resistance; the organic matter in it can increase the organic matter content of the soil, thereby improving the soil's water and fertilizer retention capacity, which is of great significance for maintaining soil ecological balance and increasing crop yields; it helps to improve the soil structure, making it more loose and breathable, which is conducive to the development of the root system and the activity of soil microorganisms, thereby improving the overall quality of the soil;

[0029] The extract of Polygonum cuspidatum has certain antibacterial and anti-inflammatory effects, which may help inhibit the growth of pathogens in the soil and reduce the occurrence of plant diseases, which has positive significance for reducing the use of chemical pesticides in agricultural production;

[0030] As an organic substance, pine needle polysaccharide can increase the organic matter content of the soil, thereby improving the soil's ability to retain water and fertilizer; the addition of pine needle polysaccharide helps to improve the soil structure, making it more loose and breathable, which is conducive to the development of the root system and the activity of soil microorganisms; pine needle polysaccharide can effectively reduce the pH value of the soil, providing a more suitable growth environment for plants; the various bioactive substances contained in pine needle polysaccharide, such as resveratrol and rhubarb, can promote plant growth;

[0031] It has certain antibacterial and anti-inflammatory effects, which may help inhibit the growth of pathogens in the soil and reduce the occurrence of plant diseases; it can increase chlorophyll content and photosynthetic rate, thereby enhancing the photosynthetic efficiency of plants.

[0032] The extract of Isatis indica contains a variety of bioactive substances, such as resveratrol and rhamnolide, which have been shown to promote plant growth and have certain antibacterial and anti-inflammatory effects, which may help inhibit the growth of pathogens in the soil, enhance plant disease resistance, and reduce the occurrence of plant diseases;

[0033] As an organic substance, it can increase the organic matter content of the soil, thereby improving the soil's ability to retain water and fertilizer. The addition of isatis indigotica extract helps to improve the soil structure, making it more loose and breathable, which is beneficial to the development of the root system and the activity of soil microorganisms.

[0034] Example 1, a biofertilizer with antibacterial effect for crop protection, comprising the following components in parts by weight: 7.5 parts of leaves, 17.5 parts of straw, 7.5 parts of rice bran, 20 parts of soybean meal, 22.5 parts of cow dung, 8 parts of fish bone meal, 3 parts of peat soil, 2.5 parts of leaf mold, 4 parts of coal gangue, 3.5 parts of diatomaceous earth, 2.5 parts of pine needle polysaccharide, 4 parts of isatis indigotica extract, and 4 parts of knotweed extract.

[0035] The present invention also discloses a method for preparing a biofertilizer with antibacterial effect for plant protection, comprising the following steps:

[0036] Step 1, weighing each component by weight;

[0037] Step 2, adding fish bone meal, organic waste, basic soil and an appropriate amount of water to the cow dung, stirring evenly, fermenting and decomposing, the fermentation temperature is 40-65° C., the fermentation time is 45-60 days, and after the fermentation is completed, spreading and airing to obtain basic bio-fertilizer;

[0038] Step three, adding pine needle polysaccharide, isatis indigo leaf extract and knotweed extract into the basic bio-fertilizer, mixing evenly again, and crushing to obtain bio-fertilizer with antibacterial effect.

[0039] Example 2, a biofertilizer with antibacterial effect for crop protection, comprising the following components in parts by weight: 5 parts of leaves, 15 parts of straw, 5 parts of rice bran, 10 parts of soybean meal, 20 parts of cow dung, 6 parts of fish bone meal, 2 parts of peat soil, 2 parts of leaf mold, 3 parts of coal gangue, 3 parts of diatomaceous earth, 2 parts of pine needle polysaccharide, 2 parts of isatis indigotica extract, and 2 parts of knotweed extract.

[0040] The present invention also discloses a method for preparing a biofertilizer with antibacterial effect for plant protection, comprising the following steps:

[0041] Step 1, weighing each component by weight;

[0042] Step 2, adding fish bone meal, organic waste, basic soil and an appropriate amount of water to the cow dung, stirring evenly, fermenting and decomposing, the fermentation temperature is 40-65° C., the fermentation time is 45-60 days, and after the fermentation is completed, spreading and airing to obtain basic bio-fertilizer;

[0043] Step three, adding pine needle polysaccharide, isatis indigo leaf extract and knotweed extract into the basic bio-fertilizer, mixing evenly again, and crushing to obtain bio-fertilizer with antibacterial effect.

[0044] Example 3, a biofertilizer with antibacterial effect for crop protection, comprising the following components in parts by weight: 10 parts of leaves, 20 parts of straw, 10 parts of rice bran, 30 parts of soybean meal, 25 parts of cow dung, 10 parts of fish bone meal, 4 parts of peat soil, 3 parts of leaf mold, 5 parts of coal gangue, 4 parts of diatomaceous earth, 3 parts of pine needle polysaccharide, 6 parts of isatis indigotica extract, and 6 parts of knotweed extract.

[0045] The present invention also discloses a method for preparing a biofertilizer with antibacterial effect for plant protection, comprising the following steps:

[0046] Step 1, weighing each component by weight;

[0047] Step 2, adding fish bone meal, organic waste, basic soil and an appropriate amount of water to the cow dung, stirring evenly, fermenting and decomposing, the fermentation temperature is 40-65° C., the fermentation time is 45-60 days, and after the fermentation is completed, spreading and airing to obtain basic bio-fertilizer;

[0048] Step three, adding pine needle polysaccharide, isatis indigo leaf extract and knotweed extract into the basic bio-fertilizer, mixing evenly again, and crushing to obtain bio-fertilizer with antibacterial effect.

[0049] The extraction process of pine needle polysaccharide is as follows:

[0050] 1. Raw material preparation: Select fresh or dried pine needles as raw materials, wash and remove impurities, and then crush them to improve the extraction efficiency of polysaccharides;

[0051] 2. Water extraction method: Mix the treated pine needle powder with water in a certain proportion, usually the solid-liquid ratio is 1:8, extract at 100°C for 3 hours, control the pH value at about 6.0, and extract the pine needle polysaccharide extract;

[0052] 3. Concentrate and dry the extract.

[0053] The extraction process of Isatis indigofera extract is as follows:

[0054] 1. Raw material preparation: Pick fresh Isatis indigofera leaves and wash them to remove surface impurities;

[0055] 2. Crushing: Dry the cleaned Isatis indigo leaves and then crush them into powder to better release their active ingredients;

[0056] 3. Water bath reflux: Mix the crushed Isatis indigo leaves with water in a certain proportion and perform water bath reflux extraction. During this process, the temperature and time need to be well controlled to ensure the full extraction of the effective ingredients.

[0057] 4. Vacuum filtration: After the extraction is completed, the extract and the residue are separated by vacuum filtration;

[0058] 5. Concentration and drying: The extract is concentrated to remove excess water, and then dried to obtain a powder or granular product of the Isatis indigotica extract.

[0059] The extraction process of Polygonum cuspidatum extract is:

[0060] 1. Raw material preparation: Select high-quality Polygonum cuspidatum rhizomes as raw materials and wash them to remove surface impurities;

[0061] 2. Crushing treatment: The cleaned rhizomes of Polygonum cuspidatum are crushed appropriately to improve the release efficiency of the active ingredients in the subsequent extraction process;

[0062] 3. Ethanol reflux extraction: Use 95% ethanol to reflux extract the crushed Polygonum cuspidatum, each extraction time is 2 hours, and it is performed twice in total to fully extract the diphenylethylene and anthraquinone compounds;

[0063] 4. Extraction: After combining the extracts, first recover the ethanol under reduced pressure until there is no alcohol smell, then use ethyl acetate for extraction to obtain stilbene compounds; the water layer is hydrolyzed by acid and then extracted with ether Soxhlet until it is colorless, and the ether layer is extracted with 5% sodium hydroxide solution and acidified to obtain anthraquinone compounds;

[0064] 5. Concentration and drying: The stilbene and anthraquinone compounds obtained above are mixed, and the contents are determined by high performance liquid chromatography (HPLC), and then concentrated and dried to finally obtain the Polygonum cuspidatum extract.

[0065] It should be noted that, in this article, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0066] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biofertilizer with antibacterial effect for crop protection, characterized in that: The invention comprises the following components in parts by weight: 40-60 parts of organic waste, 20-25 parts of animal feces, 6-10 parts of bone meal, 10-16 parts of basic soil, and 6-15 parts of natural antibacterial agent; The natural antibacterial agent comprises pine needle polysaccharide, isatis indigo plant extract and knotweed extract.

2. The biofertilizer with antibacterial effect for crop protection according to claim 1, characterized in that: The weight ratio of the pine needle polysaccharide, the isatis indigo plant extract and the knotweed extract is 2-3:2-6:2-6.

3. The biofertilizer with antibacterial effect for crop protection according to claim 2, characterized in that: The invention comprises the following components in parts by weight: 50 parts of organic waste, 22.5 parts of animal excrement, 8 parts of bone meal, 13 parts of basic soil, 2.5 parts of pine needle polysaccharide, 4 parts of isatis indigo leaf extract and 4 parts of knotweed extract.

4. The biofertilizer with antibacterial effect for crop protection according to claim 1, characterized in that: The organic waste includes leaves, straw, rice bran and soybean meal.

5. The biofertilizer with antibacterial effect for crop protection according to claim 1, characterized in that: The basic soil includes peat soil, leaf mold, coal gangue and diatomaceous earth.

6. The biofertilizer with antibacterial effect for crop protection according to claim 4, characterized in that: The organic waste comprises the following components in parts by weight: 5-10 parts of leaves, 15-20 parts of straws, 5-10 parts of rice bran and 10-30 parts of soybean meal.

7. The biofertilizer with antibacterial effect for crop protection according to claim 5, characterized in that: The basic soil comprises the following components in parts by weight: 2-4 parts of peat soil, 2-3 parts of leaf mold, 3-5 parts of coal gangue, and 3-4 parts of diatomaceous earth.

8. The biofertilizer with antibacterial effect for crop protection according to claim 1, characterized in that: The bone meal is fish bone meal.

9. The biofertilizer with antibacterial effect for crop protection according to claim 1, characterized in that: The animal excrement is cow dung.

10. A method for preparing the biofertilizer with antibacterial effect for plant protection according to claims 1 to 9, characterized in that: The steps include: Step 1, weighing each component by weight; Step 2, adding fish bone meal, organic waste, basic soil and an appropriate amount of water to the cow dung, stirring evenly, fermenting and decomposing, the fermentation temperature is 40-65° C., the fermentation time is 45-60 days, and after the fermentation is completed, spreading and airing to obtain basic bio-fertilizer; Step three, adding pine needle polysaccharide, isatis indigo leaf extract and knotweed extract into the basic bio-fertilizer, mixing evenly again, and crushing to obtain bio-fertilizer with antibacterial effect.