Portable digital constant-temperature incubator

Abstract

The invention discloses a portable digital constant-temperature incubator which comprises an incubator body shell, an upper cover and an inner container. A heat preservation module is filled between the box body shell and the inner container; a heating module is arranged on the outer side of the inner container, the incubator is provided with a temperature control module, and the temperature control module comprises a controller, a circuit board, a temperature sensor and a temperature displayer. The temperature sensor is installed on the outer wall of the inner container. The temperature control module realizes temperature monitoring, feedback and temperature control; a culture tray and a detection bag bracket are fixed in the inner container; the temperature control module is electrically connected with a power supply module, and the power supply module provides electric energy for the portable digital constant-temperature incubator. Therefore, the invention provides the portable digital constant-temperature incubator which is light, good in temperature control and capable of continuously working in the field.

Description

Technical Field

[0001] The invention relates to the field of microbial cultivation, and in particular to a portable digital constant temperature incubator. Background Art

[0002] When natural disasters such as earthquakes and floods occur, the power system is often damaged and the risk of microbial contamination increases. At this time, it is often necessary to conduct on-site testing of microbial indicators in key environmental media such as water and food. Microbial testing requires stable culture conditions, and the incubator is an important equipment that provides a constant temperature environment for microbial culture.

[0003] Traditional microbial incubators generally adopt a modular structure design, which combines the controller, heating device and working chamber together. The advantage of this design is that it can be customized and developed according to laboratory needs for equipment of different volumes; the disadvantage is that the modular structure design often requires welding, metal folding and other processes, resulting in overall weight, high energy consumption and poor temperature control. At the same time, the existing portable microbial incubators on the market are based on modular structure design, which is affected by the manufacturing process and is relatively large in size. They are mainly powered by 220V AC power, which limits the application scenarios and does not meet the requirements of outdoor portability.

[0004] Therefore, there is an urgent need to develop a portable digital constant temperature incubator that is lightweight, self-powered, and has good temperature control effects. Summary of the invention

[0005] The purpose of the present invention is to solve the above-mentioned shortcomings and defects and to provide a portable incubator.

[0006] The embodiment of the present invention discloses a portable digital constant temperature incubator, comprising: a box shell, an upper cover and an inner liner installed in the box shell; a heat preservation module is filled between the box shell and the inner liner to play a role of heat preservation and heat insulation; a heating module is arranged outside the inner liner; the heating module is embedded in the heat preservation module;

[0007] The portable digital constant temperature incubator is provided with a temperature control module, which includes a controller, a circuit board, a temperature display and a temperature sensor, and the temperature sensor is fixed on the outer wall of the inner tank; a culture tray and a detection bag bracket are fixed in the inner tank; the temperature control module is electrically connected to a power supply module.

[0008] As an implementable method, in an embodiment of the present invention, the box shell adopts a five-layer anti-collision and thermal insulation structure, and the five-layer anti-collision and thermal insulation structure is from the outside to the inside: the first layer is made of high-strength impact-resistant PE material; the second layer is made of aerogel insulation cotton; the third layer is made of PU polyurethane foam; the fourth layer is made of PP polypropylene material; the fifth layer is made of food-grade COPP material.

[0009] The portable digital constant temperature incubator disclosed in this embodiment has a box body shell made of a composite thermal insulation material that is light, rigid, has good thermal insulation performance, and has strong toughness and impact resistance. This effectively improves the thermal insulation effect while greatly reducing the weight of the incubator. The material used meets medical grade standards and has an antibacterial effect, ensuring that the sample is not contaminated by the outside world and the product itself.

[0010] As an practicable manner, in an embodiment of the present invention, in order to provide an effective volume and ensure that the weight of the incubator meets the requirements, the inner liner is made of stainless steel and is integrally formed; the inner liner is the main culture cavity.

[0011] As an implementable method, in an embodiment of the present invention, the insulation module uses aerogel insulation cotton, which is filled between the outer shell and the inner tank to ensure the internal temperature of the inner tank, reduce power consumption, and effectively extend the use time of the incubator in the field.

[0012] As an implementable method, in an embodiment of the present invention, the heating module is a dual heating system, which includes a first heating unit and a second heating unit, wherein the first heating unit is installed on the outside of the bottom of the inner tank, and the second heating unit is installed around the outer side wall of the inner tank. The heating module dual heating plate heating system is based on air thermodynamics to improve the uniformity of the spatial temperature in the portable digital constant temperature incubator.

[0013] As an implementable manner, in an embodiment of the present invention, the temperature control method of the temperature control module includes:

[0014] According to the preset collection interval, the temperature of the inner tank is continuously collected by using the temperature sensor to obtain the real-time temperature of the inner tank;

[0015] Determine whether the real-time temperature in the box is lower than a preset first temperature, and obtain a first determination result;

[0016] When the first judgment result is true, the temperature control module sends an instruction to the heating module to adopt a full-speed heating mode; the full-speed heating mode indicates that all the heating plates of the dual heating system heat the box body at maximum power; continue to execute the judgment of whether the real-time temperature in the box is lower than the preset first temperature to obtain a first judgment result;

[0017] When the first judgment result is no, the temperature deviation and the temperature deviation change value are calculated according to the preset second temperature value and the real-time temperature in the box; the preset second temperature value represents the target temperature value; the temperature deviation represents the difference between the real-time temperature in the box and the preset second temperature value; the temperature deviation change value represents the difference between the temperature deviation and the temperature deviation at the previous acquisition moment; the initial value of the deviation change value is 0;

[0018] The temperature control module calculates an output voltage value using a temperature control model, and sends the output voltage value to the heating module, and the heating module controls the dual heating system to heat according to the output voltage value;

[0019] The temperature control model is expressed as:

[0020] Δu(k)=K p ×Δe(k)+K i ×e(k)+K d ×(Δe(k)-Δe(k-1))

[0021] Among them, u(k) represents the output voltage value at time k, K p Represents the proportionality coefficient, K i Indicates the integral coefficient, K d represents the differential coefficient, e(k) represents the temperature deviation at time k, Δe(k) represents the change in temperature deviation at time k, and Δe(k-1) represents the change in temperature deviation at time k-1.

[0022] The temperature control module adopts the temperature control form of the lower computer, which can realize the temperature monitoring and control of the incubator and timely feedback response; the controller adopts a low-power single-chip microcomputer; the heating control system in the low-power single-chip microcomputer controls the current intensity of the heating module in real time, and realizes the temperature control of the constant temperature in the portable digital constant temperature incubator, thereby realizing independent temperature adjustment, effectively solving the problem of unstable temperature control of the constant temperature incubator, and having stronger adaptability; the temperature control module can adjust the output minimum holding voltage according to the surrounding environmental factors, so that the heating plate can always output a certain amount of heat and achieve thermal balance under certain conditions, so as to improve system stability and reduce volatility.

[0023] As an implementable method, in an embodiment of the present invention, the power module includes a power control circuit, a mobile power supply, and a 220V AC power adapter; the power control circuit can automatically adapt to 9V / 2A mobile power supplies and 5V / 1A mobile power supplies, can automatically monitor and adapt to commonly used mobile power supplies on the market, and can be customized and upgraded according to needs; using the 220V AC power adapter, a 220V AC power supply can be connected to charge the mobile power supply and power the microbial incubator.

[0024] As an implementable method, in an embodiment of the present invention, the mobile power supply includes two DC lithium batteries with a capacity of not less than 20,000 mAh. It should be noted that, through experiments, when two DC lithium batteries with a capacity of not less than 20,000 mAh are used, the microbial incubator can independently work for not less than 24 hours in an environment above 10°C.

[0025] The mobile power supply is embedded in an incubator, and can extend the working time in a low-temperature environment.

[0026] As an implementable method, in an embodiment of the present invention, the portable digital constant temperature incubator also includes a portable thermal insulation backpack. The portable backpack is made of composite polyester fabric on the outside, which has the characteristics of waterproof, easy to clean, wear-resistant, and shock-resistant. PE cotton and food-grade aluminum film are added inside to improve the heat insulation and thermal insulation effects; the portable backpack has the function of improving the heat insulation and thermal insulation effects.

[0027] It should be noted that the test results show that with the support of a portable backpack, the incubator can work independently for more than 24 hours at -20°C.

[0028] Compared with the prior art, the embodiments of the present invention have the following beneficial effects:

[0029] The present invention discloses a portable digital constant temperature incubator. The outer shell of the box adopts a five-layer anti-collision insulation structure, which effectively improves the insulation effect while greatly reducing the weight of the incubator. It has strong toughness and impact resistance, ensuring that the outer shell is not damaged by accidents during field operations; the material meets medical grade standards to ensure that the sample is not contaminated by the outside world and the product itself. A dual heating system design is adopted, and a temperature control module is used to achieve stable temperature control, with a stable working error of ≤±0.5℃. Using a mobile power supply method, it can work continuously in the field for no less than 24 hours, ensuring the power supply of the incubator for long-term field work, and the weight of the incubator is ≤3.0kg, realizing truly portable field culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 A schematic top view of a portable digital constant temperature incubator disclosed in an embodiment of the present invention;

[0031] Figure 2 A schematic side view of a portable digital constant temperature incubator disclosed in an embodiment of the present invention;

[0032] Figure 3 A heating plate layout method of a dual heating system disclosed in an embodiment of the present invention;

[0033] Figure 4 A schematic diagram of a dual-circuit power supply circuit connection disclosed in an embodiment of the present invention;

[0034] Figure 5 A four-point temperature sensor network disclosed in an embodiment of the present invention;

[0035] Figure 6 A schematic diagram of a circuit connection of a heat preservation module disclosed in an embodiment of the present invention;

[0036] Figure 7 The working time of a portable digital constant temperature incubator at different temperatures disclosed in an embodiment of the present invention.

[0037] Reference numerals and descriptions:

[0038] 1. Power module, 2. Circuit board, 3. Temperature display, 4. Controller, 5. Box shell, 6. Heating plate, 7. Upper cover, 8. Temperature sensor, 9. Insulation module, 10. Inner tank. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Embodiment 1

[0041] See also Figure 1 , Figure 2 . Figure 1 A schematic top view of a portable digital constant temperature incubator disclosed in an embodiment of the present invention; Figure 2 The present invention is a schematic side view of a portable digital constant temperature incubator disclosed in an embodiment of the present invention.

[0042] like Figure 1 , Figure 2 As shown, a portable digital constant temperature incubator disclosed in an embodiment of the present invention comprises a box shell 5, an upper cover 7 and an inner liner 10 installed in the box shell 5; the volume of the inner liner 10 is not less than 15 liters; an insulation module 9 is filled between the box shell 5 and the inner liner 10 to play a role in heat preservation and heat insulation; a heating module is provided outside the inner liner 10; and the heating module is embedded in the insulation module 9.

[0043] The portable digital constant temperature incubator is provided with a temperature control module, which includes a controller 4, a circuit board 2, a temperature display 3 and a temperature sensor 8; the controller 4, the circuit board 2 and the temperature display 3 are fixed on the box shell 5; the temperature sensor 8 is fixed on the outer wall of the inner liner 10; a culture tray and a detection bag holder are fixed in the inner liner 10; the culture tray is an 84-well culture tray, and the detection bag holder is a 100mL detection bag holder; the 84-well culture tray and the 100mL detection bag holder are fixed brackets adapted for the total colony count enzyme substrate method and the total coliform / Escherichia coli enzyme substrate method (qualitative detection) of the "Standard Test Method for Drinking Water" (GB / T 5750.13-2023).

[0044] The temperature control module is electrically connected to the power module 1, and the power module 1 provides power for the portable digital constant temperature incubator;

[0045] In another optional embodiment, the box shell 5 adopts a five-layer anti-collision and heat-insulating structure, which are as follows from the outside to the inside:

[0046] The first layer is made of high-strength impact-resistant PE material with a thickness of 2.5mm;

[0047] The second layer uses aerogel insulation cotton with a thickness of 2mm;

[0048] The third layer is made of PU polyurethane foam with a thickness of 12mm;

[0049] The fourth layer is made of PP polypropylene with a thickness of 2mm;

[0050] The fifth layer is made of food-grade COPP material with a thickness of 1.5mm.

[0051] First of all, since it is carried and used in the wild, the portable digital constant temperature incubator should have strong toughness and impact resistance. Therefore, in this embodiment, the first layer of PE material has wear-resistant and impact-resistant properties, and the fourth layer of PP polypropylene reinforcement layer has the property of not being easily deformed, thus achieving dual stable impact resistance. Secondly, the incubator should have good thermal insulation and environmental adaptability. Therefore, the second layer of aerogel insulation cotton is used to block the external hot and cold temperatures, and the third layer of PU polyurethane foam is used to insulate the internal cavity to create an internal temperature control environment that is not disturbed by the external environment. At the same time, the incubator should not become a source of contamination for microbial culture. Therefore, the fifth layer is filled with a food-grade COPP layer, which is a typical antibacterial material with strong antibacterial ability. The above five layers are not realized independently, and the transition between different layers is achieved by mixing and filling materials to achieve an integrated effect.

[0052] In summary, the five-layer design of the box above presents a composite thermal insulation material that is light, rigid, has good thermal insulation performance, and is tough and impact-resistant. This effectively improves the thermal insulation effect while greatly reducing the weight of the incubator. The materials used meet medical-grade standards to ensure that the samples are not contaminated by the outside world or the product itself.

[0053] In another optional embodiment, in order to provide an effective volume and make the weight of the incubator meet the requirements, the inner liner 10 is made of stainless steel and is integrally formed; the inner liner 10 is a culture cavity.

[0054] In another optional embodiment, the heat preservation module 9 uses aerogel insulation cotton, which is filled between the outer shell and the inner liner 10 to ensure the internal temperature of the inner liner 10, reduce power consumption, and effectively extend the use time of the incubator in the field. The heat preservation module 9 achieves the heat preservation effect by thickening the polyurethane and using high-density heat insulation materials between the inner and outer layers.

[0055] Aerogel insulation cotton has become one of the most important new thermal insulation materials due to its structural and functional characteristics such as low density, large specific surface area, high porosity and low thermal conductivity. In recent years, the thermal insulation performance of aerogel composite materials has been applied in many fields such as military aerospace, energy storage and conversion, etc. New progress has been made. Aerogel material is a three-dimensional lightweight network structure solid synthetic material with a nanoporous structure formed by the mutual aggregation of nano-scale particles or polymer molecular chains, and the pores are filled with gaseous dispersion medium. Due to its structural characteristics such as large specific surface area, high porosity, and ultra-low density, it exhibits excellent properties such as low thermal conductivity, low refractive index and low sound propagation speed, making it have great application prospects in many fields such as thermal insulation, photoelectric catalysis, sound insulation and noise reduction, light transmission and refraction, adsorption separation, energy storage and conversion, etc.

[0056] The culture box formed in the above embodiment was tested for its heat preservation (cold preservation) performance.

[0057] Thermal insulation performance: Place the opening of the sample in a (20±5)℃ environment for more than 30 minutes, then fill the sample with (80±3)℃ water, cover the lid, tighten the lock, and place it in an environmental chamber at 0℃ to 5℃, and test how long it takes for the water temperature to drop to 30℃.

[0058] Cold preservation performance: Place the sample opening in a (20±5)℃ environment for more than 30 minutes, then fill the sample with ice cubes, cover the lid, tighten the lock, and place it in a (35±5)℃ environmental chamber to test how long it takes for the ice cubes to melt.

[0059] The test results are shown in Table 1. The box has good thermal insulation and heat preservation effects.

[0060] Table 1 Insulation (cooling) performance

[0061]

[0062] In another optional embodiment, the heating module is a dual heating system, which includes a first heating unit and a second heating unit, wherein the first heating unit is installed on the outside of the bottom of the inner tank 10, and the second heating unit is installed around the outside of the side wall of the inner tank 10. The heating module dual heating plate heating system is based on air thermodynamics to improve the uniformity of the spatial temperature in the portable digital constant temperature incubator.

[0063] Assume that the air in the thermostat is ideal air and exchanges heat by natural convection. According to the heat calculation formula:

[0064] Q=CVρΔt

[0065] Where Q is the heat required for the temperature change; C is the specific heat capacity, the value is C = 1030 J / kg·℃; V is the volume of the gas in the constant temperature box; ρ is the air density, the value is P = 10.29 kg / m3; △t is the temperature change in the constant temperature box, take 1℃, according to Joule's law:

[0066] Q′=Pt=Q

[0067] Where Q' is the heat generated, P is the power provided by the electric heating plate, and t is the required time. Substituting the numerical value into the above formula, it is found that the time required for the electric heating plate with a power of 18×5W to heat the constant temperature box by 1°C is about 3S, which can basically achieve a constant temperature control effect.

[0068] At the same time, considering that there should not be large air flow and temperature dead corners in the incubator, according to the law of air heat conduction, the dual heating system uses six heating plates 6, the layout of which is as follows: Figure 3 As shown, explanation is required Figure 3 The left picture is a side view. Figure 3 The right side is a top view, the first heating unit includes two heating plates at the bottom of the inner tank 10, and the second heating unit includes four heating plates around the outer side wall of the inner tank 10. At the same time, a dual-circuit power supply design is adopted, such as Figure 4 As shown, the 24V of the battery is stepped down and stabilized to DC9V through this circuit, which achieves stable heating while reducing power consumption. It can continuously output a large current of 2A, and the built-in power supply can work for no less than 36 hours at room temperature.

[0069] In another optional embodiment, the temperature sensor 8 is an NTC thermistor temperature sensor; using high-precision multi-channel temperature acquisition technology, temperature sensor chips are placed at the four corners of the box to form a sensor network to collect the temperature inside the box, so as to accurately measure the temperature inside the portable digital constant temperature incubator. By using four-point temperature measurement, the measurement error caused by uneven temperature distribution can be reduced to a great extent. The structure of the four-point temperature sensor network is as follows: Figure 5 shown.

[0070] In another optional embodiment, the temperature control module adopts the lower computer temperature control form, and the controller 4 adopts a low-power single-chip microcomputer; the insulation module circuit is connected as follows Figure 6 As shown, Figure 6 This is a circuit connection diagram of a heat preservation module disclosed in an embodiment of the present invention. The heating control system in the low-power single-chip microcomputer controls the current intensity of the heating plate in real time to achieve the temperature control of the thermostat in the portable digital thermostat incubator, specifically:

[0071] According to the preset collection interval, the temperature of the inner tank 10 is continuously collected using a temperature sensor to obtain the real-time temperature of the inner tank 10; it should be noted that the system is in standby mode after power-on. After the power is turned on, the system is first initialized, which includes setting the RTC real-time clock, initializing preset parameters, etc. After initialization, the interrupt method is used to realize real-time temperature collection every 1 second.

[0072] Determine whether the real-time temperature in the box is lower than a preset first temperature, and obtain a first determination result;

[0073] When the first judgment result is true, the temperature control module sends an instruction to the heating module to adopt a full-speed heating mode; the full-speed heating mode indicates that all the heating plates of the dual heating system heat the box body at maximum power; continue to execute the judgment of whether the real-time temperature in the box is lower than the preset first temperature to obtain a first judgment result;

[0074] When the first judgment result is no, the temperature deviation and the temperature deviation change value are calculated according to the preset second temperature value and the real-time temperature in the box; the preset second temperature value represents the target temperature value; the temperature deviation represents the difference between the real-time temperature in the box and the preset second temperature value; the temperature deviation change value represents the difference between the temperature deviation and the temperature deviation at the previous acquisition moment; the initial value of the deviation change value is 0;

[0075] The temperature control module calculates an output voltage value using a temperature control model, and sends the output voltage value to the heating module, and the heating module controls the dual heating system to heat according to the output voltage value;

[0076] The temperature control model is expressed as:

[0077] Δu(k)=K p ×Δe(k)+K i ×e(k)+K d ×(Δe(k)-Δe(k-1))

[0078] Among them, u(k) represents the output voltage value at time k, K p Represents the proportionality coefficient, K i Indicates the integral coefficient, K d represents the differential coefficient, e(k) represents the temperature deviation at time k, Δe(k) represents the change in temperature deviation at time k, and Δe(k-1) represents the change in temperature deviation at time k-1.

[0079] The temperature control module adopts the temperature control form of the lower computer, which can realize the timely feedback response of the incubator temperature monitoring and control; the controller 4 adopts a low-power single-chip microcomputer; the heating control system in the low-power single-chip microcomputer controls the current intensity of the heating module in real time, and realizes the temperature control of the incubator in the portable digital constant temperature incubator, thereby realizing the autonomous adjustment of the temperature, effectively solving the problem of unstable temperature control of the incubator, and having stronger adaptability; the temperature control module can adjust the output minimum holding voltage according to the surrounding environmental factors, so that the heating plate can always output a certain amount of heat, achieve thermal balance under certain conditions, so as to improve the system stability and reduce volatility. The above method has the advantages of simple principle, convenient operation and high control accuracy, and is suitable for systems such as constant temperature incubators with large inertia, large hysteresis and difficult to establish accurate mathematical models.

[0080] The temperature control module is designed based on the coupling of digital temperature sensor and single-chip control, which directly converts the temperature signal into a digital signal, and controls the current intensity of the heating plate in real time through the temperature control model, thereby realizing autonomous temperature regulation, effectively solving the problem of unstable temperature control of the constant temperature box, and having stronger adaptability; the temperature control module can adjust the minimum holding voltage of the DA output according to the surrounding environmental factors, so that the heating plate can always output a certain amount of heat and achieve thermal balance under certain conditions, so as to improve system stability and reduce volatility.

[0081] In this implementation, when the deviation between the real-time temperature and the set temperature is large, the thermostat adopts the full-speed heating mode to quickly reduce the temperature difference; when the temperature difference is small, the thermostat uses the temperature control model algorithm to implement the precise temperature control mode for precise temperature control. The critical point of the temperature difference between the two modes is comprehensively selected based on factors such as the temperature change time, the maximum temperature process quantity, and the temperature control accuracy after stabilization. The temperature fluctuation under stable conditions is shown in Table 2.

[0082] Table 2 Temperature fluctuations under stable conditions

[0083]

[0084] In another optional embodiment, the power module 1 includes a power control circuit, a mobile power supply, and a 220V AC power adapter; the power control circuit can automatically adapt to a 9V / 2A mobile power supply and a 5V / 1A mobile power supply, can automatically monitor and adapt to commonly used mobile power supplies on the market, and can be customized and upgraded according to needs; using the 220V AC power adapter, a 220V AC power supply can be connected to charge the mobile power supply and power the microbial incubator.

[0085] In another optional embodiment, the mobile power supply uses two DC lithium batteries with a capacity of not less than 20,000 mAh. It should be noted that, through experiments, when two DC lithium batteries with a capacity of not less than 20,000 mAh are used, the microbial incubator can independently work for not less than 24 hours in an environment above 4°C and for not less than 18 hours in an environment above -20°C, and the mobile power supply can work in an environment of -40°C-45°C.

[0086] It should be noted that the constant temperature incubator described in this embodiment was used to test the working time at different temperatures. The results are as follows: Figure 7 shown.

[0087] The mobile power source is embedded in the thermal insulation layer of the incubator, and can extend the working time in a low-temperature environment.

[0088] In yet another optional embodiment, the portable digital constant temperature incubator also includes a portable thermal insulation backpack.

[0089] The exterior of the portable backpack is made of composite polyester fabric, which has the characteristics of being waterproof, easy to clean, wear-resistant, and shock-resistant. PE cotton and food-grade aluminum film are added inside to improve the heat insulation and thermal insulation effects. The portable backpack has the function of improving the heat insulation and thermal insulation effects.

[0090] It should be noted that the experimental results show that with the support of a portable backpack, the incubator can work independently for more than 24 hours at -20°C.

[0091] Finally, it should be noted that the portable digital constant temperature incubator disclosed in the embodiment of the present invention is only a preferred embodiment of the present invention, which is only used to illustrate the technical solution of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, it should be understood by those skilled in the art that the technical solutions described in the aforementioned embodiments can still be modified, or some of the technical features therein can be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A portable digital constant temperature incubator, characterized in that: include: A box shell, an upper cover and an inner liner installed in the box shell; a heat preservation module is filled between the box shell and the inner liner; a heating module is arranged outside the inner liner; The portable digital constant temperature incubator is provided with a temperature control module, which includes a controller, a circuit board, a temperature display and a temperature sensor, and the temperature sensor is installed on the outer wall of the inner tank; a culture tray and a detection bag bracket are fixed in the inner tank; the temperature control module is electrically connected to a power module.

2. The portable digital constant temperature incubator according to claim 1, characterized in that: The box shell adopts a five-layer anti-collision and heat-insulating structure; the five-layer anti-collision and heat-insulating structure is as follows from the outside to the inside: the first layer is made of high-strength impact-resistant PE material; the second layer is made of aerogel insulation cotton; the third layer is made of PU polyurethane foam; the fourth layer is made of PP polypropylene material; the fifth layer is made of food-grade COPP material.

3. The portable digital constant temperature incubator according to claim 1, characterized in that: The inner tank is made of stainless steel and is integrally formed.

4. The portable digital constant temperature incubator according to claim 1, characterized in that: The heat preservation module adopts aerogel heat insulation cotton, which is filled between the outer shell and the inner liner.

5. The portable digital constant temperature incubator according to claim 1, characterized in that: The heating module is a dual heating system, which includes a first heating unit and a second heating unit. The first heating unit is installed on the outside of the bottom of the inner pot, and the second heating unit is installed around the outside of the side wall of the inner pot.

6. The portable digital constant temperature incubator according to claim 5, characterized in that: The temperature control method of the temperature control module comprises: According to the preset collection interval, the temperature of the inner tank is continuously collected by using the temperature sensor to obtain the real-time temperature of the inner tank; Determine whether the real-time temperature in the box is lower than a preset first temperature, and obtain a first determination result; When the first judgment result is true, the temperature control module sends an instruction to the heating module to adopt a full-speed heating mode; the full-speed heating mode indicates that the dual heating system heats the box body at maximum power; the judgment of whether the real-time temperature in the box is lower than a preset first temperature is continued to obtain a first judgment result; When the first judgment result is no, the temperature deviation and the temperature deviation change value are calculated according to the preset second temperature value and the real-time temperature in the box; the preset second temperature value represents the target temperature value; the temperature deviation represents the difference between the real-time temperature in the box and the preset second temperature value; the temperature deviation change value represents the difference between the temperature deviation and the temperature deviation at the previous acquisition moment; the initial value of the deviation change value is 0; The temperature control module calculates an output voltage value using a temperature control model, and sends the output voltage value to the heating module, and the heating module controls the dual heating system to heat according to the output voltage value; The temperature control model is expressed as: Δu(k)=K p ×Δe(k)+K i ×e(k)+K d ×(Δe(k)-Δe(k-1)) Among them, u(k) represents the output voltage value at time k, K p Represents the proportionality coefficient, K i Indicates the integral coefficient, K d represents the differential coefficient, e(k) represents the temperature deviation at time k, Δe(k) represents the change in temperature deviation at time k, and Δe(k-1) represents the change in temperature deviation at time k-1.

7. The portable digital constant temperature incubator according to claim 1, characterized in that: The temperature sensor is an NTC thermistor temperature sensor.

8. The portable digital constant temperature incubator according to claim 1, characterized in that: The power module includes a power control circuit, a mobile power supply, and an AC power adapter; the power control circuit automatically adapts to a 9V / 2A mobile power supply and a 5V / 1A mobile power supply; the AC power adapter is used to connect to an AC power supply.

9. The portable digital constant temperature incubator according to claim 8, characterized in that: The mobile power supply comprises two direct current lithium batteries with a capacity of not less than 20000 mAH; the mobile power supply is embedded in the incubator.

10. The portable digital constant temperature incubator according to claim 1, characterized in that: The portable digital constant temperature incubator also includes a portable thermal insulation backpack.

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