Blood coagulation analyzer and magnetic bead detection channel thereof

Abstract

The invention discloses a blood coagulation analyzer and a magnetic bead detection channel thereof. The magnetic bead detection channel comprises a magnetic bead detection block, a coil driving assembly and an infrared light detection assembly, the magnetic bead detection block is used for placing a reaction cup filled with a detected sample and magnetic beads; the coil driving assembly is arranged on the magnetic bead detection block and is used for driving the magnetic beads to swing back and forth in the reaction cup; the infrared light detection assembly is arranged on the magnetic bead detection block and is used for emitting and receiving infrared light to a preset position of a magnetic bead swinging track, so that the infrared light is blocked in the process that the magnetic beads swing back and forth in the reaction cup, and the swinging condition of the magnetic beads in the reaction cup is detected. According to the scheme, the magnetic beads are driven to swing back and forth in an induction coil driving mode, and the operation state of the magnetic beads is detected in an infrared light emitting and receiving detection mode; the infrared light signal is strong, and the received signal does not need to be subjected to multi-stage amplification processing, so that the magnetic bead detection channel circuit is simple in design, low in cost, higher in stability and better in magnetic bead detection effect.

Description

Technical Field

[0001] The present invention relates to the technical field of magnetic bead detection of coagulation analyzers, and in particular to a coagulation analyzer and a magnetic bead detection channel thereof. Background Art

[0002] Coagulation analyzers often use optical methods to detect the coagulation or fibrinolytic properties of blood samples. However, for some special blood, there is interference with the detection of light, resulting in inaccurate analysis results. Therefore, a detection method based on the magnetic bead method has emerged. This method converts the fibrinogen in the blood sample into cross-linked fibrin by adding a certain reagent to the blood sample, simulating the coagulation process of the reagent blood. The reaction coagulation time is determined by continuously monitoring the changes in the physical (viscosity and fluidity) properties of the reaction system during this process, and is used as the conversion time of fibrinogen. This principle is used to determine the coagulation or fibrinolytic properties of blood samples.

[0003] The magnetic bead detection channel of the existing coagulation analyzer based on the coagulation method detects the swing of the magnetic beads through a dual magnetic circuit; wherein the dual magnetic circuit consists of two sets of coils, front and back, left and right. The front and back sets of coils drive the magnetic beads to swing back and forth, and the left and right sets of coils detect the swing of the magnetic beads.

[0004] Among them, the existing magnetic bead detection channel detects the swing of the magnetic beads through the coil. The detection signal is an analog signal, and the signal is weak and needs to go through multiple stages of amplification and filtering, which makes the circuit design of the magnetic bead detection channel complex, costly, and unstable. In addition, since the existing magnetic bead detection channel uses coils for both driving and detection, the coil parameters are relatively large, and there are also problems of difficulty and complexity in design, as well as complex welding and installation. Summary of the invention

[0005] In view of this, the present invention provides a magnetic bead detection channel, which can make the circuit design of the magnetic bead detection channel simple, low cost, higher stability, and better magnetic bead detection effect; at the same time, this also reduces the use of coils, which can help reduce the difficulty and complexity of the design and production operation of the magnetic bead detection channel.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A magnetic bead detection channel, applied to a coagulation analyzer, comprises: a magnetic bead detection block, a coil drive component and an infrared light detection component;

[0008] The magnetic bead detection block is used to place a reaction cup containing a sample to be tested and magnetic beads;

[0009] The coil driving component is disposed on the magnetic bead detection block and is used to drive the magnetic beads to swing back and forth in the reaction cup;

[0010] The infrared light detection component is arranged on the magnetic bead detection block, and is used to emit and receive infrared light to a preset position of the magnetic bead swing track, so that the magnetic bead blocks the infrared light during the swinging back and forth in the reaction cup, thereby detecting the swinging condition of the magnetic bead in the reaction cup.

[0011] Preferably, a placement groove is provided on the top of the magnetic bead detection block, and is used to place the reaction cup;

[0012] The coil driving assembly is built into the magnetic bead detection block, and is located on the third side and the fourth side opposite to the placement slot and is connected to the placement slot;

[0013] The infrared light detection component is built into the magnetic bead detection block, and is located on the first and second sides opposite to the placement slot and is connected to the placement slot; wherein the relative direction of the first and second sides of the placement slot is perpendicular to the relative direction of the third and fourth sides.

[0014] Preferably, the infrared light detection component comprises: an infrared light emitting circuit board and an infrared light receiving circuit board;

[0015] The infrared light emitting circuit board is embedded in the first side wall of the magnetic bead detection block, is connected to the placement groove and is used to emit the infrared light to the middle position of the magnetic bead swing track;

[0016] The infrared light receiving circuit board is embedded in the second side wall of the magnetic bead detection block, is communicated with the placement groove and is used to receive the infrared light emitted by the infrared light emitting circuit board.

[0017] Preferably, the first side wall of the magnetic bead detection block is provided with a first mounting groove, and the second side wall is provided with a second mounting groove;

[0018] The infrared light emitting circuit board is a patch type infrared light emitting circuit board, and is fitted in the first mounting groove of the magnetic bead detection block;

[0019] The infrared light receiving circuit board is a patch type infrared light receiving circuit board and is fitted and installed in the second installation groove of the magnetic bead detection block.

[0020] Preferably, the patch-type infrared light emitting circuit board is provided with an infrared light emitting diode, and is used to emit the infrared light to the middle position of the swing track of the magnetic bead;

[0021] The patch-type infrared light receiving circuit board is provided with an infrared light receiving tube and is used for receiving the infrared light emitted by the infrared light emitting diode.

[0022] Preferably, a third installation groove is formed on the third side wall of the magnetic bead detection block, and a fourth installation groove is formed on the fourth side wall, and both are connected to the placement groove;

[0023] The coil driving assembly comprises: a first induction coil and a second induction coil;

[0024] The first induction coil and the second induction coil are respectively installed in the third installation groove and the fourth installation groove of the magnetic bead detection block, and are used to apply an alternating magnetic field to the magnetic beads in the reaction cup.

[0025] Preferably, it also includes a switching circuit board;

[0026] The adapter circuit board is arranged on the third side wall of the magnetic bead detection block, has pins and is used to be plugged into the main control circuit board of the coagulation analyzer;

[0027] The infrared light transmitting circuit board, the infrared light receiving circuit board, the first induction coil and the second induction coil are all connected to the main control circuit board for communication via the adapter circuit board.

[0028] Preferably, the enameled wire of the first induction coil is welded to the adapter circuit board;

[0029] A wiring groove is provided on the top of the magnetic bead detection block, and two ends of the wiring groove are respectively connected to the third installation groove and the fourth installation groove;

[0030] The enameled wire of the second induction coil passes through the wiring groove and is welded to the adapter circuit board.

[0031] Preferably, it also includes: a voltage comparator and a filter;

[0032] The infrared light receiving circuit board transmits the received infrared light signal to the voltage comparator through the adapter circuit board; when the magnetic bead blocks the infrared light during the back-and-forth swinging process, the voltage comparator outputs a high level; when the magnetic bead does not block the infrared light during the back-and-forth swinging process, the voltage comparator outputs a low level; the voltage comparator is also used to transmit the obtained two levels to the main control circuit board after filtering by the filter.

[0033] A coagulation analyzer comprises a magnetic bead detection channel, wherein the magnetic bead detection channel is the magnetic bead detection channel described above.

[0034] It can be seen from the above technical solutions that the magnetic bead detection channel provided by the present invention adopts an induction coil driving method to drive the magnetic beads to swing back and forth, and adopts an infrared light emission and reception detection method to detect the operating status of the magnetic beads; wherein, the infrared light signal is strong, and there is no need to perform multi-stage amplification processing on the received signal, which can make the circuit design of the magnetic bead detection channel simple, low-cost, and more stable, and the magnetic bead detection effect is better; at the same time, this also reduces the use of coils, which can help reduce the difficulty and complexity of the magnetic bead detection channel design. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0036] Figure 1 A schematic diagram of the structure of a magnetic bead detection channel provided in an embodiment of the present invention;

[0037] Figure 2 Another schematic diagram of the structure of the magnetic bead detection channel provided in an embodiment of the present invention;

[0038] Figure 3 An exploded diagram of the structure of a magnetic bead detection channel provided in an embodiment of the present invention;

[0039] Figure 4 A schematic diagram of the structure of a coil driving component and an infrared light detection component provided in an embodiment of the present invention;

[0040] Figure 5 Another structural schematic diagram of a coil driving component and an infrared light detection component provided in an embodiment of the present invention;

[0041] Figure 6 A schematic diagram of magnetic bead detection provided by an embodiment of the present invention;

[0042] Figure 7 A schematic diagram of the structure of a magnetic bead detection block provided in an embodiment of the present invention;

[0043] Figure 8 Another structural schematic diagram of a magnetic bead detection block provided in an embodiment of the present invention;

[0044] Fig. 9 A schematic diagram of the relationship between the swing amplitude and the swing time of the magnetic beads provided in an embodiment of the present invention;

[0045] Fig.10 A schematic diagram of the principle of a magnetic bead detection circuit provided in an embodiment of the present invention.

[0046] Among them, 1 is a SMD infrared light receiving circuit board, 1.1 is an infrared light receiving tube, 2 is a magnetic bead detection block, 2.1 is a first mounting slot, 2.2 is a second mounting slot, 2.3 is a third mounting slot, 2.4 is a fourth mounting slot, 2.5 is a placement slot, 2.6 is a wiring slot, 3 is a first induction coil, 4 is a SMD infrared light emitting circuit board, 4.1 is an infrared light emitting diode, 5 is a transfer circuit board, 5.1 is a pin, 6 is a magnetic bead, 7 is a reaction cup, 8 is a second induction coil, 9 is a voltage comparator, 10 is a filter, 11 is a coil drive chip, 12 is an infrared light control switch, and 13 is an MCU. DETAILED DESCRIPTION

[0047] Regarding the magnetic bead detection channel mentioned in the above background technology, its detection method actually has the following disadvantages:

[0048] 1) The coil detects the swing of the magnetic bead. The detection signal is an analog signal, and the signal is weak. It needs to go through multiple stages of amplification and filtering, which makes the circuit design complex, costly, and unstable.

[0049] 2) During the production process of the magnetic bead detection channel, the enameled wire of the coil is very thin and is easy to break during welding and installation, making the operation process difficult and complicated;

[0050] 3) During the design process of the magnetic bead detection channel, there are many coil parameters, including wire diameter, number of turns, winding method, etc., so the design is difficult and complex;

[0051] 4) During the production process of the magnetic bead detection channel, the coil has many parameters and has a great impact on the magnetic bead detection performance. Therefore, the material control and detection processes are very strict, which increases the complexity of production and detection.

[0052] To this end, this solution provides a magnetic bead detection channel for a coagulation analyzer that can overcome the above-mentioned defects.

[0053] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0054] The magnetic bead detection channel provided in the embodiment of the present invention is applied to a coagulation analyzer, such as Figure 1 and Figure 2 As shown, it includes: a magnetic bead detection block 2, a coil driving component and an infrared light detection component;

[0055] The magnetic bead detection block 2 is used to place a reaction cup 7 containing a sample to be tested and magnetic beads 6;

[0056] The coil driving assembly is disposed on the magnetic bead detection block 2 and is used to drive the magnetic bead 6 to swing back and forth in the reaction cup 7;

[0057] The infrared light detection component is arranged on the magnetic bead detection block 2 and is used to emit and receive infrared light to a preset position of the swinging track of the magnetic bead 6. The magnetic bead 6 will block the infrared light during the swinging back and forth in the reaction cup 7, thereby detecting the swinging of the magnetic bead 6 in the reaction cup 7.

[0058] It should be noted that if Figure 6 As shown, the sample to be tested contained in the reaction cup 7 is added with reagents;

[0059] The coil driving component is an induction coil driving component, and the induction coil driving component can be a dual induction coil driving component, which can be located on both sides (such as the left and right sides) of the reaction cup 7 when it is arranged in the magnetic bead detection block 2, and is used to apply an alternating magnetic field to the magnetic beads 6 in the reaction cup 7 to drive the magnetic beads 6 to swing back and forth in the reaction cup 7, and the coil driving component can be connected to the MCU13 of the main control circuit board of the coagulation analyzer for communication, so that the operation of the coil driving component can be controlled by the MCU13;

[0060] When the infrared light detection component is arranged on the magnetic bead detection block 2, it can be located on the other two sides (such as the front and back sides) of the reaction cup 7, and is used to emit and receive infrared light to a preset position (such as the middle position) of the swinging track of the magnetic bead 6, so that the magnetic bead 6 blocks the infrared light during the swinging back and forth in the reaction cup 7, thereby detecting the swinging state of the magnetic bead 6 in the reaction cup 7, and detecting the changes in the viscosity and fluidity of the reaction system to be tested; wherein, the infrared light detection component includes: an infrared light emitting part and an infrared light receiving part; the infrared light emitting part is used to emit infrared light to a preset position of the swinging track of the magnetic bead 6, and the infrared light receiving part is used to receive the infrared light emitted by the infrared light emitting part, and the infrared light detection component can also be connected to the MCU13 of the main control circuit board of the coagulation analyzer, that is, the infrared light emitting part can be controlled by the MCU13 to emit infrared light, and the signal (voltage signal) sent by the infrared light receiving part can be received by the MCU13, and the coagulation time of the sample to be tested can be obtained by analyzing and processing in combination with the algorithm; of course, if Fig. 9As shown, as the state of the sample under test changes, the swing amplitude and swing speed of the magnetic bead 6 will also change. According to this information combined with the algorithm, the coagulation time of the sample under test can be analyzed and calculated; wherein, the infrared light receiving part can convert the received light signal into a voltage signal, and this voltage is an analog quantity, and as the intensity of the received infrared light changes dynamically, the analog voltage can also be directly divided into two states, such as a high level and a low level, by the voltage comparator 9, so that the MCU13 can more conveniently identify and calculate; for example, when the magnetic bead 6 blocks the infrared light, the voltage comparator 9 outputs a high level (such as 3.3V), and when the magnetic bead 6 does not block the infrared light, the voltage comparator 9 outputs a low level (such as 0V), and then the voltage comparator 9 transmits these two voltage signals to the MCU13 (with the function of processing signals), and the MCU13 analyzes and processes them in combination with the algorithm to obtain the coagulation time of the sample under test; wherein, the algorithm can calculate the coagulation time of the sample under test according to the distribution state of the high level and the low level; of course, the process can adopt the existing technology.

[0061] That is to say, this scheme provides a magnetic bead detection channel for a coagulation analyzer, which can realize the coagulation function test of blood samples. It adopts an induction coil driving method to drive the magnetic beads to swing back and forth, and adopts an infrared light emission and reception detection method to detect the operating status of the magnetic beads; wherein, the infrared light signal is strong, and there is no need to perform multi-stage amplification processing on the received signal, which can make the circuit design of the magnetic bead detection channel simple, low-cost, and more stable, and the magnetic bead detection effect is better; at the same time, this also reduces the use of coils, which can help reduce the difficulty and complexity of the magnetic bead detection channel design, as well as the complexity of production operations and installation.

[0062] In this scheme, if Figure 7 As shown, a placement slot 2.5 is provided on the top of the magnetic bead detection block 2 and is used to place a reaction cup 7;

[0063] like Figure 1 As shown, the coil driving assembly is built into the magnetic bead detection block 2, and is located on the third side and the fourth side opposite to the placement slot 2.5 and is connected to the placement slot 2.5;

[0064] The infrared light detection component is built into the magnetic bead detection block 2, and is located on the first and second sides opposite to the placement slot 2.5 and is connected to the placement slot 2.5; wherein the relative direction of the first and second sides of the placement slot 2.5 is perpendicular to the relative direction of the third and fourth sides.

[0065] It should be noted that the dual induction coil assembly as described above can be respectively arranged on the third side and the fourth side opposite to the placement slot 2.5 and connected to the placement slot 2.5, so as to apply an alternating magnetic field to the magnetic beads 6 in the reaction cup 7; the infrared light emitting part and the infrared light receiving part of the infrared light detection assembly can be respectively arranged on the first side and the second side opposite to the placement slot 2.5 and connected to the placement slot 2.5, that is, they can be respectively located on both sides of the swing track of the magnetic beads 6; wherein, the first side and the second side of the placement slot 2.5 can be the front side and the rear side respectively, and the third side and the fourth side can be the left side and the right side respectively, and to ensure that the dual induction coil assembly can be completely built into the magnetic bead detection block 2, the connection line between the third side and the fourth side of the placement slot 2.5 is parallel to the length direction of the magnetic bead detection block 2.

[0066] That is to say, the coil driving component and the infrared light detection component are both built into the magnetic bead detection block 2, and are respectively located on the corresponding opposite sides of the placement slot 2.5, so that the coil driving component and the infrared light detection component can be integrated into the magnetic bead detection block 2, which helps to achieve a miniaturized design of the magnetic bead detection channel.

[0067] Specifically, the infrared light detection assembly includes: an infrared light transmitting circuit board and an infrared light receiving circuit board;

[0068] like Figure 1 As shown, the infrared light emitting circuit board is embedded in the first side wall of the magnetic bead detection block 2, and is connected to the placement groove 2.5 and is used to emit infrared light to the middle position of the swing track of the magnetic bead 6;

[0069] like Figure 2 As shown, the infrared light receiving circuit board is embedded in the second side wall of the magnetic bead detection block 2, and is connected to the placement groove 2.5 and is used to receive the infrared light emitted by the infrared light emitting circuit board.

[0070] It should be noted that the infrared light emitting circuit board is the infrared light emitting part of the infrared light detection component mentioned above; the infrared light receiving circuit board is the infrared light receiving part of the infrared light detection component mentioned above; wherein the first side wall of the magnetic bead detection block 2 is located on the first side of the placement groove 2.5, and the second side wall is located on the second side of the placement groove 2.5, such as Figure 1 As shown, the infrared light emitting circuit board can be embedded in the front side wall of the magnetic bead detection block 2, and is connected to the placement groove 2.5 and is used to emit infrared light to the middle lowest position of the swing track of the magnetic bead 6; of course, the infrared light emitting circuit board has an infrared light emitting device, and can be connected to the MCU13 for communication, so that the emission of the infrared light emitting circuit board can be controlled by the MCU13; Figure 2As shown, the infrared light receiving circuit board can be embedded in the rear side wall of the magnetic bead detection block 2, and is connected to the placement groove 2.5 and is used to receive the infrared light emitted by the infrared light emitting device; of course, the infrared light emitting circuit board and the infrared light receiving circuit board are located on both sides of the magnetic bead swing track so that the magnetic beads can block the infrared light during the back and forth swinging process, and the infrared light receiving circuit board has an infrared light receiving device and can be connected to the MCU13 for communication, so that the signal sent by the infrared light receiving circuit board can be received through the MCU13, and then analyzed and processed in combination with the algorithm to obtain the coagulation time of the sample to be tested; in addition, the above scheme is designed in this way, which is convenient for the installation of the infrared light emitting circuit board and the infrared light receiving circuit board, and also helps to obtain a better magnetic bead detection effect.

[0071] Furthermore, if Figure 7 and Figure 8 As shown, the first side wall of the magnetic bead detection block 2 is provided with a first mounting groove 2.1, and the second side wall is provided with a second mounting groove 2.2;

[0072] like Figure 3 and Figure 1 As shown, the infrared light emitting circuit board is a patch type infrared light emitting circuit board 4, and is fitted in the first mounting groove 2.1 of the magnetic bead detection block 2;

[0073] like Figure 3 and Figure 2 As shown, the infrared light receiving circuit board is a patch type infrared light receiving circuit board 1 , and is fitted and installed in the second installation groove 2 . 2 of the magnetic bead detection block 2 .

[0074] It should be noted that if Figure 7 As shown, the front side wall of the magnetic bead detection block 2 may be provided with a first mounting groove 2.1. Figure 8 As shown, the rear side wall of the magnetic bead detection block 2 may be provided with a second mounting groove 2.2; Figure 1 As shown, the infrared light emitting circuit board adopts a patch type infrared light emitting circuit board 4, and can be installed in the first installation groove 2.1 of the magnetic bead detection block 2 by means of a screw assembly; Figure 2 As shown, the infrared light receiving circuit board adopts a patch type infrared light receiving circuit board 1, and can be installed in the second installation groove 2.2 of the magnetic bead detection block 2 by a screw assembly. In this way, it can not only avoid the infrared light emitting circuit board and the infrared light receiving circuit board from protruding from the corresponding side walls of the magnetic bead detection block 2, but also realize the compact design of the magnetic bead detection block 2 and the infrared light detection assembly. Of course, multiple magnetic bead detection channels can also be arranged side by side, and the infrared light emitting circuit board and the infrared light receiving circuit board can be easily installed, while facilitating the inspection and maintenance of the infrared light emitting circuit board and the infrared light receiving circuit board.

[0075] Furthermore, if Figure 5As shown, the SMD infrared light emitting circuit board 4 is provided with an infrared light emitting diode 4.1, and is used to emit infrared light to the middle position of the swing track of the magnetic bead 6;

[0076] like Figure 4 As shown, the SMD infrared light receiving circuit board 1 is provided with an infrared light receiving tube 1.1 and is used to receive the infrared light emitted by the infrared light emitting diode 4.1.

[0077] It should be noted that the infrared light emitting diode 4.1 is the infrared light emitting device of the infrared light emitting circuit board mentioned above, and the infrared light receiving tube 1.1 is the infrared light receiving device of the infrared light receiving circuit board mentioned above; wherein, the infrared light emitting diode 4.1 is located on the side of the patch type infrared light emitting circuit board 4 close to the placement slot 2.5, and is used to emit infrared light to the middle lowest position of the swing track of the magnetic bead 6; of course, the magnetic bead detection block 2 is provided with a light-transmitting hole between the infrared light emitting diode 4.1 and the placement slot 2.5, and the opening and closing of the infrared light emitting diode 4.1 can be controlled by MCU13; the infrared light receiving tube 1.1 is located on the side of the patch type infrared light receiving circuit board 1 close to the placement slot 2.5, and is used to receive the infrared light emitted by the infrared light emitting diode 4.1; of course, the infrared light receiving tube 1.1 is located on the opposite side of the infrared light emitting diode 4.1, and the magnetic bead detection block 2 is also provided with a light-transmitting hole between the infrared light receiving tube 1.1 and the placement slot 2.5, and the signal sent by the infrared light receiving tube 1.1 can be received by MCU13. That is to say, the infrared light detection component has relatively distributed infrared light emitting devices and infrared light receiving devices, which can make the detection of the swing of the magnetic beads stable and reliable.

[0078] In this scheme, if Figure 7 and Figure 8 As shown, the third side wall of the magnetic bead detection block 2 is provided with a third installation groove 2.3, and the fourth side wall is provided with a fourth installation groove 2.4, and both are connected to the placement groove;

[0079] like Figure 3 As shown, the coil driving assembly includes: a first induction coil 3 and a second induction coil 8;

[0080] like Figure 1 and Figure 2 As shown, the first induction coil 3 and the second induction coil 8 are respectively installed in the third installation groove 2.3 and the fourth installation groove 2.4 of the magnetic bead detection block 2, and are used to apply an alternating magnetic field to the magnetic beads 6 in the reaction cup 7.

[0081] It should be noted that the third side wall and the fourth side wall of the magnetic bead detection block 2 can be the left side wall and the right side wall respectively, that is, the first induction coil 3 and the second induction coil 8 can be respectively built-in on the left and right sides of the placement groove 2.5 to drive the magnetic beads 6 to swing back and forth between the left and right sides of the reaction cup 7, and the first induction coil 3 and the second induction coil 8 can control the supply of alternating current through the MCU13; in addition, the shape of the third mounting groove 2.3 can match the shape of the first induction coil 3, and the shape of the fourth mounting groove 2.4 can match the shape of the second induction coil 8, to ensure that each induction coil is stably installed in the corresponding mounting groove, such as Figure 7 and Figure 8 As shown, the third mounting groove 2.3 and the fourth mounting groove 2.4 can be cylindrical mounting grooves. Of course, the coil drive assembly is built into the third side and the fourth side of the placement groove 2.5, which helps to achieve a compact design of the magnetic bead detection channel. In addition, the first induction coil 3 includes: a magnetic core rod, a skeleton (PA66 skeleton) and an enameled wire; wherein the skeleton is sleeved on the magnetic core rod, and its first end is flush with the first end of the magnetic core rod, and the second end is close to the second end of the magnetic core rod; that is, the second end of the magnetic core rod protrudes outside the skeleton; the enameled wire is evenly wound on the outer peripheral wall of the skeleton; the third side wall of the placement groove 2.5 is provided with a positioning through hole connected to the third mounting groove 2.3. When the first induction coil 3 is installed in the third mounting groove 2.3, the second end of the magnetic core rod is positioned and installed in the positioning through hole and connected to the placement groove 2.5; similarly, the structural composition and connection relationship of the second induction coil 8 are the same as those of the first induction coil 3, and it is also installed in the fourth mounting groove 2.4 in the above manner, which will not be repeated here.

[0082] That is to say, the working principle of the magnetic bead detection channel of this coagulation analyzer is: a pair of induction coils are used to drive the magnetic beads to swing back and forth, and a pair of infrared light emitting and receiving circuit boards are used to detect the swinging state of the magnetic beads, thereby detecting changes in the viscosity and fluidity of the reaction system to be tested.

[0083] Among them, Fig. 9As shown, a magnetic bead 6 is placed in the reaction system to be tested, and an alternating magnetic field is applied to the magnetic bead 6 through a pair of induction coils on the left and right sides of the reaction cup 7, so as to drive the magnetic bead 6 to swing back and forth in the reaction cup 7; the infrared light detection component includes: a patch type infrared light emitting circuit board 4 and a patch type infrared light receiving circuit board 1, which are respectively vertically mounted on the front and rear sides of the swing direction of the magnetic bead 6. The swinging process of the magnetic bead 6 will block the infrared light path, and the time t (t= d / v, where the diameter of the light-transmitting hole is d, and the speed of the magnetic beads 6 passing through the light-transmitting hole is v), t is stable within a certain range before blood coagulation; when cross-linked fibrin is not formed, the coil drive assembly drives the magnetic beads 6 to keep swinging back and forth along the curved track at the bottom of the reaction cup 7; during the coagulation reaction, fibrinogen is converted into cross-linked fibrin, and the viscosity and fluidity of the reaction system gradually change, and the swinging of the magnetic beads 6 will be significantly hindered until it can no longer swing, thereby causing the time t that the magnetic beads 6 block the infrared light to change. After blood coagulation, the magnetic beads may stop at the position of the light-transmitting hole or within the position range on both sides of the light-transmitting hole. At this time, according to the sudden change of t, it can be judged that the magnetic beads 6 tend to stop; by obtaining the time t that the magnetic beads 6 block the infrared light through the light-transmitting hole during the swinging process, combined with algorithm analysis, the time from swinging to the swinging tending to be stationary can be obtained, which is used as the coagulation time. Among them, when the two states of occlusion and non-occlusion change alternately, it means that the magnetic beads are swinging, which means that the sample to be tested has not solidified; and when the state changes from alternating to a single state (the single state is non-occluded or occluded), it means that the magnetic beads are no longer swinging, and the time for the state to change alternately is the coagulation time.

[0084] Furthermore, if Figure 3 As shown, the magnetic bead detection channel provided in the embodiment of the present invention further includes a switching circuit board 5;

[0085] like Figure 1 As shown, the adapter circuit board 5 is arranged on the third side wall of the magnetic bead detection block 2, and has a pin 5.1 and is used to be plugged into the main control circuit board of the coagulation analyzer;

[0086] The infrared light transmitting circuit board, the infrared light receiving circuit board, the first induction coil 3 and the second induction coil 8 are all connected to the main control circuit board for communication via the adapter circuit board 5 .

[0087] It should be noted that the adapter circuit board 5 can be installed on the third side wall (such as the left side wall) of the magnetic bead detection block 2 by means of a screw assembly, and the pin 5.1 it has is used to be plugged into the main control circuit board of the coagulation analyzer to achieve communication connection between the adapter circuit board 5 and the main control circuit board, and the two leads of the infrared light emitting circuit board and the two leads of the infrared light receiving circuit board are communicatively connected to the adapter circuit board 5, and the first induction coil 3 and the second induction coil 8 are electrically connected to the adapter circuit board 5. Among them, Figure 1 and Figure 2As shown, the adapter circuit board 5 has four groups of pins 5.1, that is, eight pins 5.1, of which two pins 5.1 are related to the driving of the induction coil, one pin 5.1 is related to the power supply of the induction coil, one pin 5.1 is related to GND, two pins 5.1 are related to the infrared light receiving tube 1.1, and two pins 5.1 are related to the infrared light emitting diode 4.1.

[0088] That is to say, the adapter circuit board 5, as the adapter circuit board of the magnetic bead detection block 2, is not only convenient for assembling the magnetic bead detection channel with the main control circuit board, but also convenient for the communication connection between the coil drive component and the infrared light detection component and the main control circuit board respectively. Of course, the infrared light emitting circuit board, the infrared light receiving circuit board, the first induction coil 3 and the second induction coil 8 can also be directly connected to the main control circuit board for communication, but the circuit structure will be relatively complicated.

[0089] In addition, the circuit design of magnetic bead detection of the coagulation analyzer is as follows Fig.10 As shown, MCU13 can provide a 12V driving voltage for the two induction coils of the magnetic bead test channel through the coil driving chip 11. The two induction coils will alternately drive the swing of the magnetic beads in each cycle to achieve the effect of driving the magnetic beads to swing back and forth; wherein, MCU13 is communicatively connected to the coil driving chip 11, the coil driving chip 11 is communicatively connected to the adapter circuit board 5, and the adapter circuit board 5 is electrically connected to the two induction coils.

[0090] Furthermore, the enameled wire of the first induction coil 3 is welded to the adapter circuit board 5;

[0091] A wiring groove 2.6 is provided on the top of the magnetic bead detection block 2, and its two ends are respectively connected to the third installation groove 2.3 and the fourth installation groove 2.4;

[0092] The enameled wire of the second induction coil 8 passes through the wiring groove 2 . 6 and is welded to the adapter circuit board 5 .

[0093] It should be noted that if Figure 1 As shown, the enameled wire of the first induction coil 3 close to the adapter circuit board 5 can be welded to the adapter circuit board 5 nearby, while the enameled wire of the second induction coil 8 far from the adapter circuit board 5 is routed through the routing groove 2.6 and welded to the adapter circuit board 5. This can prevent the enameled wires of the two induction coils from touching the circuit boards on both sides, and will not affect the heating of the test block below and the placement of the reaction cup 7 above. At the same time, it can also prevent the enameled wires of the induction coils from breaking and wearing. Among them, as Figure 7 and Figure 8 As shown, the third mounting groove 2.3 and the fourth mounting groove 2.4 can pass through the top of the adapter circuit board 5, so that one end of the wiring groove 2.6 is connected to the third mounting groove 2.3 and the other end is connected to the fourth mounting groove 2.4, and the wiring groove 2.6 can be a U-shaped wiring groove.

[0094] In addition, if Figure 7 and Figure 8 As shown, the first mounting groove 2.1 and the second mounting groove 2.2 of the magnetic bead detection block 2 both pass through the third side wall of the magnetic bead detection block 2, so as to facilitate the communication connection between the leads of the infrared light emitting circuit board and the infrared light receiving circuit board and the adapter circuit board 5.

[0095] In this scheme, if Fig.10 As shown, the magnetic bead detection channel provided in the embodiment of the present invention further includes: a voltage comparator 9 and a filter 10;

[0096] The infrared light receiving circuit board transmits the received infrared light signal to the voltage comparator 9 through the adapter circuit board 5; when the magnetic bead 6 blocks the infrared light during the back-and-forth swinging process, the voltage comparator 9 outputs a high level; when the magnetic bead 6 does not block the infrared light during the back-and-forth swinging process, the voltage comparator 9 outputs a low level; the voltage comparator 9 is also used to transmit the obtained two levels to the main control circuit board after filtering by the filter 10.

[0097] It should be noted that if Fig.10 As shown, MCU13 can control the infrared light emitting diode 4.1 of the patch type infrared light emitting circuit board 4 to turn on through the infrared light control switch 12, and receive infrared light through the infrared light receiving tube 1.1 of the patch type infrared light receiving circuit board 1 on the opposite side, so as to detect the running state and speed of the magnetic beads, and transmit the received infrared light signal to the voltage comparator 9 through the adapter circuit board 5, so that when the magnetic beads block the infrared light, the voltage comparator 9 outputs a high level, and when the infrared light is not blocked, the voltage comparator 9 outputs a low level, and the voltage comparator 9 will obtain the two levels after filtering through the filter 10 and then transmit them to MCU13, and MCU13 will analyze and process them in combination with the algorithm, and then the coagulation time and other information of the sample to be tested can be obtained. Among them, MCU13 receives two voltage signals of high level and low level, and then analyzes and calculates them according to the distribution state of high level and low level in combination with the algorithm to obtain the coagulation time. In addition, the voltage comparator 9, the filter 10, the coil drive chip 11, the infrared light control switch 12 and the MCU13 can all be arranged on the main control circuit board. In addition, the user can also control the MCU13 through the interface to open or close several magnetic bead test channels simultaneously or individually to perform coagulation test.

[0098] That is to say, the magnetic bead detection channel provides the driving force for the magnetic beads to swing back and forth through a group of induction coils (which can be distributed on the left and right sides of the reaction cup), and detects the swinging state of the magnetic beads through the side infrared light detection components (which can be distributed on the front and back sides of the reaction cup). Compared with the existing magnetic bead detection technology, the design and operation process of the magnetic bead detection channel is simpler and greatly simplifies the complexity of the production and detection process.

[0099] Among them, the structure and circuit design of the magnetic bead test channel of the coagulation analyzer are simple. It only requires a set of induction coils and a set of surface-mount infrared light transmitting and receiving circuit boards, which greatly simplifies the difficulty and complexity of design and production. The welding and installation process of the induction coil is simple and convenient, and it is not easy to wear and break. It is also easy to operate and install.

[0100] The embodiment of the present invention further provides a coagulation analyzer, including a magnetic bead detection channel, wherein the magnetic bead detection channel is the magnetic bead detection channel described above. Since the present solution adopts the magnetic bead detection channel described above, it also has corresponding beneficial effects, which can be specifically referred to the previous description and will not be repeated here.

[0101] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0102] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A magnetic bead detection channel, applied to a coagulation analyzer, characterized in that: include: Magnetic bead detection block (2), coil drive component and infrared light detection component; The magnetic bead detection block (2) is used to place a reaction cup (7) containing a sample to be tested and magnetic beads (6); The coil driving component is arranged on the magnetic bead detection block (2) and is used to drive the magnetic bead (6) to swing back and forth in the reaction cup (7); The infrared light detection component is arranged on the magnetic bead detection block (2) and is used to emit and receive infrared light to a preset position of the swing track of the magnetic bead (6), so that the magnetic bead (6) blocks the infrared light during the swing back and forth in the reaction cup (7), thereby detecting the swing of the magnetic bead (6) in the reaction cup (7).

2. The magnetic bead detection channel according to claim 1, characterized in that: The top of the magnetic bead detection block (2) is provided with a placement groove (2.5) for placing the reaction cup (7); The coil drive assembly is built into the magnetic bead detection block (2), and is located on a third side and a fourth side opposite to the placement slot (2.5) and is in communication with the placement slot (2.5); The infrared light detection component is built into the magnetic bead detection block (2), and is located on the first side and the second side opposite to the placement slot (2.5) and is in communication with the placement slot (2.5); wherein the relative direction between the first side and the second side of the placement slot (2.5) is perpendicular to the relative direction between the third side and the fourth side.

3. The magnetic bead detection channel according to claim 2, characterized in that: The infrared light detection assembly comprises: an infrared light transmitting circuit board and an infrared light receiving circuit board; The infrared light emitting circuit board is embedded in the first side wall of the magnetic bead detection block (2), is connected to the placement groove (2.5), and is used to emit the infrared light to the middle position of the swing track of the magnetic bead (6); The infrared light receiving circuit board is embedded in the second side wall of the magnetic bead detection block (2), is connected to the placement groove (2.5), and is used to receive the infrared light emitted by the infrared light emitting circuit board.

4. The magnetic bead detection channel according to claim 3, characterized in that: The first side wall of the magnetic bead detection block (2) is provided with a first installation groove (2.1), and the second side wall is provided with a second installation groove (2.2); The infrared light emitting circuit board is a patch-type infrared light emitting circuit board (4), and is fitted and mounted in the first mounting groove (2.1) of the magnetic bead detection block (2); The infrared light receiving circuit board is a patch-type infrared light receiving circuit board (1), and is fitted and mounted in the second mounting groove (2.2) of the magnetic bead detection block (2).

5. The magnetic bead detection channel according to claim 4, characterized in that: The patch-type infrared light emitting circuit board (4) is provided with an infrared light emitting diode (4.1) and is used to emit the infrared light toward the middle position of the swing track of the magnetic bead (6); The patch-type infrared light receiving circuit board (1) is provided with an infrared light receiving tube (1.1) and is used for receiving the infrared light emitted by the infrared light emitting diode (4.1).

6. The magnetic bead detection channel according to claim 3, characterized in that: The third side wall of the magnetic bead detection block (2) is provided with a third installation groove (2.3), and the fourth side wall is provided with a fourth installation groove (2.4), and both are connected to the placement groove; The coil drive assembly comprises: a first induction coil (3) and a second induction coil (8); The first induction coil (3) and the second induction coil (8) are respectively installed in the third installation groove (2.3) and the fourth installation groove (2.4) of the magnetic bead detection block (2), and are used to apply an alternating magnetic field to the magnetic beads (6) in the reaction cup (7).

7. The magnetic bead detection channel according to claim 6, characterized in that: Also includes a transfer circuit board (5); The adapter circuit board (5) is arranged on the third side wall of the magnetic bead detection block (2), has pins (5.1) and is used for being plugged into the main control circuit board of the coagulation analyzer; The infrared light transmitting circuit board, the infrared light receiving circuit board, the first induction coil (3) and the second induction coil (8) are all connected to the main control circuit board for communication via the adapter circuit board (5).

8. The magnetic bead detection channel according to claim 7, characterized in that: The enameled wire of the first induction coil (3) is welded to the adapter circuit board (5); A wiring groove (2.6) is provided on the top of the magnetic bead detection block (2), and two ends of the wiring groove are respectively connected to the third installation groove (2.3) and the fourth installation groove (2.4); The enameled wire of the second induction coil (8) passes through the wiring groove (2.6) and is welded to the adapter circuit board (5).

9. The magnetic bead detection channel according to claim 7, characterized in that: Also includes: A voltage comparator (9) and a filter (10); The infrared light receiving circuit board transmits the received infrared light signal to the voltage comparator (9) via the adapter circuit board (5); wherein, when the magnetic bead (6) blocks the infrared light during the back-and-forth swinging process, the voltage comparator (9) outputs a high level; and when the magnetic bead (6) does not block the infrared light during the back-and-forth swinging process, the voltage comparator (9) outputs a low level; the voltage comparator (9) is further used to transmit the obtained two levels to the main control circuit board after filtering by the filter (10).

10. A coagulation analyzer, comprising a magnetic bead detection channel, characterized in that: The magnetic bead detection channel is the magnetic bead detection channel as described in any one of claims 1-9.

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