Microscopic imaging assembly and single cell operation device

Abstract

The invention discloses a microscopic imaging assembly and a single cell operation device, and the microscopic imaging assembly comprises a sliding pair which is located below an objective table and comprises a sliding part and a sliding seat; the lens base is provided with a lens, the lens base is rotationally arranged, and in the sliding direction, the lens base is provided with a first position close to the objective table and a second position opposite to the first position; the lens base has a plurality of orientations, at least one of the orientations is downward, one of the lenses is aligned to the objective table, and the lens is focused on a sample on the objective table in the first position; an image detection unit; the first driving device drives the mirror base to enter a first position or a second position; when the mirror base enters the second position, the first coupling piece and the second coupling piece are coupled and can drive the mirror base to change the direction, and when the mirror base leaves the second position, the first coupling piece and the second coupling piece are separated. According to the invention, rapid switching of the lens base can be realized, accurate focusing is ensured, the use experience is improved, and automatic control is realized.

Description

Technical Field

[0001] The present application relates to the field of cell manipulation technology, and in particular to a microscopic imaging component and a single cell manipulation device. Background Art

[0002] When conducting cell research, it is necessary to observe the specific morphology of the cells and extract the target cells separately for research. The commonly used method for single-cell operation is in situ extraction, which specifically uses a suction needle to suck in a single cell for spatial position transfer. During the operation, the front end opening of the microtube probe used for cell extraction is about 1 to 20 microns, and the extraction and release of a single cell is achieved through changes in air pressure inside the microtube.

[0003] In the process of cell manipulation, a microscopic imaging component is required to provide a window for the probe. For different scenes, it is often necessary to switch lenses with different magnifications or functions. In order to ensure the control accuracy during the lens switching process, the lens switching process in the prior art is cumbersome and the switching structure is complex, leaving room for improvement. Summary of the invention

[0004] In order to solve the above technical problems, the present application discloses a microscopic imaging assembly for imaging a stage, including: A sliding pair, defining a sliding direction relative to the stage, the sliding pair comprising a sliding member and a sliding seat; A lens holder is provided with a plurality of lenses, and the lens holder is rotatably provided on the sliding member; in the sliding direction, the lens holder has a first position close to the stage and a second position opposite thereto; in the rotating direction, the lens holder has a plurality of orientations, and in at least one orientation, one of the lenses is aligned with the stage, and in the first position, the lens is focused on the sample on the stage; An image detection unit is located below the lens base, and the lens imaging path runs through the lens base and forms an image through the image detection unit; A first driving device, comprising a first power source and a linkage member, wherein the linkage member drives the mirror holder to enter the first position or the second position under the action of the first power source; The second driving device includes a second power source, a first coupling member linked to the second power source, and a second coupling member arranged on the mirror base. When the mirror base enters the second position, the first coupling member and the second coupling member are coupled and the second power source can drive the mirror base to change the orientation. When the mirror base leaves the second position, the first coupling member and the second coupling member are separated.

[0005] Several optional methods are also provided below, but they are not intended to be additional limitations on the above-mentioned overall solution, but are merely further supplements or preferences. Under the premise that there are no technical or logical contradictions, each optional method can be combined with the above-mentioned overall solution separately, and multiple optional methods can also be combined.

[0006] In one embodiment, the first driving device also includes a driving track arranged in the same direction as the sliding pair, and the linkage member includes a base moving on the driving track and a driving arm extending from the base toward the mirror seat, and the driving arm is used to drive the mirror seat into the first position.

[0007] In one embodiment, the linkage member and the mirror base are unidirectionally transmitted, the mirror base is lifted by the linkage member to enter the first position, and is driven by gravity to fall back to the second position.

[0008] In one embodiment, the mirror seat is rotatably disposed on the sliding member via a rotating shaft, the rotating shaft passes through the sliding pair and the second coupling member is disposed on the end portion.

[0009] In one of the embodiments, an open mating groove is provided on the upper portion of the first coupling member in the sliding direction of the sliding pair. When the mirror mount enters the second position, at least a portion of the second coupling member enters the mating groove to achieve circumferential coupling between the second power source and the rotating shaft. When the mirror mount exits the second position, the second coupling member exits the mating groove to achieve separation.

[0010] In one embodiment, the first coupling member is linked to a circumferential trigger member, the second driving device further includes a detector responsive to the circumferential trigger member, and the second power source is controlled by the detector.

[0011] In one embodiment, the sliding pairs are provided with two arranged side by side and enclose a receiving area, the mirror seat is located in the receiving area and connected to the sliding members of each sliding pair, and the first driving device and the second driving device are respectively located on both sides of the receiving area.

[0012] In one embodiment, the lens seat is rotatably mounted on the lens frame via a rotating shaft, the lens frame is U-shaped and both sides of the U-shape are respectively connected to the sliding members of each sliding pair, the rotating shaft is disposed on both sides of the U-shaped opening of the lens frame and the lens seat is accommodated inside the U-shape.

[0013] In one of the embodiments, the lens holder is provided with a plurality of mounting surfaces, each mounting surface is used to mount the lens and a limiting portion extending outward, and the frame is provided with a blocking member for cooperating with the limiting portion, and when the lens holder is adjusted to a preset orientation, the limiting portion and the blocking member abut against each other to accurately position the orientation of the lens holder.

[0014] The present application also discloses a single cell operation device, comprising: A stage for fixing the container carrying the sample; A probe assembly, comprising a probe and a holder for holding the probe, wherein the probe is an elongated hollow structure having a front end and a rear end opposite to each other, wherein the front end of the probe extends to the container and the rear end of the probe is inserted into the holder when in use; a control assembly, including a moving platform for moving the probe assembly in space to adjust the position of the probe; A microscopic imaging component, which is the microscopic imaging component described in the above technical solution of the present application and provides a window for positioning the probe; An air pressure source assembly is connected to the probe via the holder to provide positive and / or negative air pressure to the probe.

[0015] The technical solution disclosed in the present application realizes the motion constraint of the mirror mount in different directions through the rotational cooperation of the sliding pair and the mirror mount, thereby realizing rapid switching of the mirror mount under the cooperation of the first drive device and the second drive device, and providing a basis for the automated control of the microscopic imaging assembly while ensuring precise focus and improving the user experience.

[0016] The specific beneficial technical effects will be further explained in the specific implementation methods in combination with specific structures or steps. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the structure of a microscopic imaging component in one embodiment of the present application; Figure 2 for Figure 1 Schematic diagram of the coordination of the various parts of the microscopic imaging assembly; Figure 3 It is a schematic structural diagram of a first driving device of a microscopic imaging assembly; Figure 4 It is a schematic diagram of the structure of the second driving device of the microscopic imaging assembly; Figure 5 It is a schematic diagram of the coordination between the first driving device, the second driving device and the mirror holder; Figure 6 is a schematic cross-sectional view of a microscopic imaging component; Figure 7 This is a schematic diagram of a single cell manipulation device in one embodiment of the present application; Figure 8 A schematic diagram of the air pressure source assembly of the single-cell manipulation device; Fig. 9 This is a working schematic diagram of the air pressure source component.

[0018] The reference numerals in the figures are described as follows: 10. stage; 21. optical microscope objective lens; 22. image detection unit; 23. light source; 50. mobile platform; 60. air pressure source assembly; 61. plunger pump; 61a. inlet end; 61b. outlet end; 63c. plunger; 62. solenoid valve; 62a. common end; 62b. normally open end; 62c. normally closed end; 63. three-way connector; 64. first one-way valve; 65. second one-way valve; 66. air filter; 67. silencer; 68. plug; 70. control system; 100. Probe; 200, holder; 400, sliding pair; 401, sliding member; 402, sliding seat; 403, sliding bracket; 4031, adapter window; 404, accommodating area; 410, mirror seat; 411, lens; 412, rotating shaft; 413, limiting portion; 414, mirror frame; 4141, matching protrusion; 415, blocking member; 420, first driving device; 421, first power source; 422, linkage member; 4221, base; 4222, driving arm; 424, first driving frame; 430, second driving device; 431, second power source; 432, first coupling member; 4321, matching groove; 433, second coupling member; 434, second driving frame; 435, circumferential trigger member; 4351, notch; 436, detector. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0020] It should be noted that when a component is referred to as being "connected" to another component, it may be directly connected to the other component or there may be a central component. When a component is referred to as being "disposed on" another component, it may be directly disposed on the other component or there may be a central component at the same time.

[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0022] Reference Figure 1 To Attachment Figure 7As shown, the present application discloses a microscope imaging assembly for imaging a stage 10, including a sliding pair 400 located below the stage 10, a mirror mount 410 rotatably disposed on the sliding pair 400, an image detection unit 22 located below the mirror mount 410, and a first driving device 420 and a second driving device 430 for driving the mirror mount 410 to move.

[0023] The sliding pair 400 defines the sliding direction relative to the stage 10. The sliding pair 400 includes a sliding member 401 and a sliding seat 402. The sliding seat 402 is a linear guide extending in the direction of gravity. The sliding member 401 is matched with the linear guide. A plurality of lenses 411 are arranged on the lens seat 410. The lens seat 410 is rotatably arranged on the sliding member 401. In the sliding direction, the lens seat 410 has a first position close to the stage 10 and a second position opposite to the stage 10 (for example, near the stage 10). Figure 1 ). In the direction of rotation, the lens holder 410 has several orientations, and in at least one orientation, one of the lenses 411 is aligned with the stage 10. Different lenses may have different first positions due to different focal lengths. For each lens, in the first position, the lens 411 focuses on the sample on the stage 10. The lens 411 realizes imaging through the image detection unit 22. The imaging path of the lens 411 runs through the lens holder 410. The interior of the lens holder 410 is hollow, and the overall structure is a cylindrical structure to prevent external light from mixing into the imaging path to affect the imaging quality.

[0024] The first driving device 420 includes a first power source 421 and a linkage member 422. The linkage member 422 drives the mirror base 410 to enter the first position or the second position under the action of the first power source 421. The second driving device 430 includes a second power source 431, a first coupling member 432 linked to the second power source 431, and a second coupling member 433 disposed on the mirror base 410. When the mirror base 410 enters the second position, the first coupling member 432 and the second coupling member 433 are coupled and the second power source 431 can drive the mirror base 410 to change its orientation. When the mirror base 410 leaves the second position, the first coupling member 432 and the second coupling member 433 are separated.

[0025] The technical solution disclosed in the present application realizes motion constraints of the mirror base 410 in different directions through the rotational cooperation of the sliding pair 400 and the mirror base 410, thereby realizing rapid switching of the mirror base 410 under the cooperation of the first drive device 420 and the second drive device 430, thereby ensuring precise focus and improving the user experience while providing a basis for the automated control of the microscopic imaging assembly.

[0026] For details on the configuration of the first driving device 420, please refer to the attached Figure 1 and attached Figure 3In the illustrated embodiment, the first driving device 420 further includes a first power source 421 which is a motor and drives the linkage member 422 to move. The linkage member 422 includes a base 4221 and a driving arm 4222 extending from the base 4221 toward the mirror base 410, and the driving arm 4222 is used to drive the mirror base 410 to enter the first position. The driving arms 4222 are arranged in groups, and each group of driving arms 4222 is provided with two driving arms and forms a U-shaped opening with the base 4221, and the sliding pair 400 is located in the U-shaped opening formed by the driving arms 4222. The end of the driving arm 4222 can directly cooperate with the mirror base 410, or can achieve indirect cooperation with the mirror base 410 through the mirror frame 414. For example Figure 5 In the embodiment, the mirror base 410 is mounted on the mirror frame 414, and the mirror frame 414 is provided with a mating protrusion 4141 that cooperates with the driving arm 4222, and the end of the driving arm 4222 interacts with the lower surface of the mating protrusion 4141 to drive the mirror base 410. Further, the linkage member 422 and the mirror base 410 are unidirectionally transmitted, and the mirror base 410 is lifted by the linkage member 422 to enter the first position, and is driven by gravity to fall back to the second position. In a specific installation method, the first driving device 420 also includes a first driving frame 424 for installing the first power source 421, and the first driving frame 424 has a mating plane parallel to the sliding direction of the sliding pair 400. When the first power source 421 drives the mirror base 410 to move in the sliding direction of the sliding pair 400, it can adjust the relative distance between the lens 411 and the stage 10, thereby realizing the focusing action.

[0027] For details on the configuration of the second driving device 430, please refer to the attached Figure 2 and attached Figure 4In the embodiment shown, the mirror base 410 is rotatably disposed on the sliding member 401 via a rotating shaft 412, the rotating shaft 412 penetrates the sliding pair 400 and a second coupling member 433 is disposed on the end thereof. Further, in the sliding direction of the sliding pair 400, an open matching groove 4321 is disposed on the upper portion of the first coupling member 432, when the mirror base 410 enters the second position, at least a portion of the second coupling member 433 enters the matching groove 4321 to achieve circumferential coupling between the second power source 431 and the rotating shaft 412, when the mirror base 410 exits the second position, the second coupling member 433 exits the matching groove 4321 to achieve separation. In the figure, the first coupling member 432 is a cylinder, the matching groove 4321 is formed by a local concave on the circumferential surface of the cylinder and has a first driving surface and a second driving surface opposite to each other in the circumferential direction of the cylinder, and accordingly, the shape of the second coupling member 433 is complementary to the matching groove 4321 and is mutually attached to the first driving surface and the second driving surface to achieve coupling. The sliding pair 400 includes a sliding bracket 403 located at one side of the mirror base 410. The sliding bracket 403 is provided with an adapting window 4031 for the rotation shaft 412 to pass through. The first coupling member 432 and the second coupling member 433 cooperate with each other through the adapting window 4031. When the mirror base 410 moves between the first position and the second position, the second coupling member 433 moves relative to the first coupling member 432 in the adapting window 4031.

[0028] In terms of installation details, the second drive device 430 includes a second drive frame 434 for mounting a second power source 431, and the second power source 431 is a motor with a controllable rotation angle. The second power source 431 is connected to the first coupling member 432 through a reduction mechanism to achieve precise control of the movement angle of the first coupling member 432. The reduction mechanism includes a driving wheel connected to the second power source 431, a driven wheel connected to the first coupling member 432, and a transmission belt arranged between the driving wheel and the driven wheel. The second drive frame 434 is a double-layer structure with a cavity inside, and the reduction mechanism is arranged in the cavity to avoid being exposed to the outside.

[0029] In order to further accurately control the orientation of the lens holder 410 to ensure the imaging quality of the lens 411, the closed-loop control of the second driving device 430 can be implemented, for example, referring to the attached Figure 4 In the illustrated embodiment, the first coupling member 432 is linked to a circumferential trigger member 435. The circumferential trigger member 435 in the figure is a disc-shaped structure and rotates with the first coupling member 432. The circumferential trigger member 435 is provided with a plurality of identification parts to indicate its own circumferential position. Each identification part can be uniformly set to realize the identification of relative position, or can be set differently to realize the identification of absolute position. The identification part can be set as a notch 4351 with reference to the figure. The second driving device 430 also includes a detector 436 that responds to the circumferential trigger member 435. The second power source 431 is controlled by the detector 436. The specific control principle can be implemented in combination with the existing technology and will not be repeated here.

[0030] In addition to the precise control of the driving device, the motion accuracy of the mirror holder 410 can also be further ensured by mechanical limit. Figure 5 and attached Figure 6 In the embodiment shown, the lens holder 410 is rotatably mounted on the lens frame 414 via a rotating shaft 412. The lens holder 410 is provided with a plurality of mounting surfaces, each of which is used to mount a lens 411 and a stopper 413 extending outward. The lens frame 414 is provided with a blocking member 415 for cooperating with the stopper 413. When the lens holder 410 is adjusted to a preset orientation, the stopper 413 and the blocking member 415 abut against each other to accurately position the orientation of the lens holder 410. The lens frame 414 is U-shaped and both sides of the U-shape are respectively connected to the sliding members 401 of each sliding pair 400. The rotating shaft 412 is provided on both sides of the U-shaped opening of the lens holder 414 and the lens holder 410 is accommodated inside the U-shaped opening. The blocking member 415 is located at the bottom of the U-shaped opening of the lens frame 414. The stopper is located at the connection between adjacent mounting surfaces and extends away from the mounting surface. When the lens holder 410 rotates relative to the lens frame 414, the blocking member 415 is located on the movement path of the limiting member to achieve the cooperation between the two. In the figure, there are two blocking members 415, which are respectively located on both sides of the lens frame 414. The two blocking members 415 cooperate with two different limiting members on the lens holder 410 to determine different directions of the lens holder 410.

[0031] For the overall layout, please refer to the attached Figure 2 In the illustrated embodiment, the sliding pair 400 is provided with two accommodating areas 404 arranged side by side and enclosed to form a accommodating area 404, the mirror seat 410 is located in the accommodating area 404, the mirror seat 410 is connected to the sliding member 401 of each sliding pair 400 through the mirror frame 414, the first driving device 420 and the second driving device 430 are respectively located on both sides of the accommodating area 404, that is, the first driving device 420 and the mirror seat 410 are located on both sides of one of the sliding pairs 400, and the second driving device and the mirror seat 410 are located on both sides of the other sliding pair 400. The first driving device 420 drives the mirror seat 410 by cooperating with the mirror frame 414 through the driving arm 4222 of the linkage member 422 around the two sides of the sliding pair 400; the second driving device 430 realizes the coupling or separation of the first coupling member 432 and the second coupling member 433 through the rotating shaft 412 penetrating the sliding bracket 403.

[0032] Based on the above, combined with the attached Figure 7 In one embodiment of the present application, a single cell manipulation device is also disclosed, comprising: The stage 10 is used to fix the container carrying the sample; The probe 100 assembly includes the probe 100 and a holder 200 for holding the probe 100. The probe 100 is an elongated hollow structure having a front end and a rear end opposite to each other. When in use, the front end of the probe 100 extends to the container, and the rear end of the probe 100 is inserted into the holder 200. A control assembly, including a moving platform 50 for moving the probe 100 assembly in space to adjust the position of the probe 100; A microscopic imaging component, which is the microscopic imaging component in each of the above embodiments and provides a window for the positioning probe 100; The air pressure source assembly 60 is connected to the probe 100 via the holder 200 to provide positive and / or negative air pressure to the probe 100 .

[0033] The microscopic imaging assembly further includes a light source 23, an optical microscope objective lens 21 (i.e., the lens 411 mentioned above), and a CCD camera (i.e., the image detection unit 22 mentioned above). The light source is generally disposed directly above the stage 10 and is as shown in FIG. Figure 7 The light source 23 emits light in the direction indicated by the arrow below. The optical microscope objective lens 21 and the image detection unit 22 are located below the stage. Figure 7 The light is transmitted in the direction indicated by the arrow between the optical microscope objective lens 21 and the image detection unit 22 and an image is formed on the image detection unit 22. The control system 70 can be a terminal device such as a computer. The plunger pump 61 in the air pressure source assembly 60, the solenoid valve 62, the motor in the mobile platform 50, the motor in the stage 10, and the motor of the driving device in the microscopic imaging assembly are controlled by the control system. The control system also has an imaging display unit, that is, the probe 100 and the target cells can be displayed on the screen through the imaging system, and the operator can perform real-time operations.

[0034] In another embodiment, the air pressure source assembly 60 is used as a power source for aspirating and spitting out substances (cells, reagents, etc.). Figure 8 In the illustrated embodiment, the air pressure source assembly includes a plunger pump 61, a solenoid valve 62, a three-way connector 63, a first one-way valve 64, a second one-way valve 65, an air filter 66, a muffler 67, and a plug 68. The plunger pump 61 has an inlet end 61a, an outlet end 61b, and a plunger 61c; the solenoid valve 62 has a common end 62a, a normally open end 62b, and a normally closed end 62c; the air filter 66 has an air inlet end and an air outlet end; the first one-way valve 64 has an "IN" port and an "OUT" port; the second one-way valve 65 has an "IN" port and an "OUT" port; and the three-way connector 63 has three connection ports.

[0035] The plug 68 is screwed into the inlet end 61a of the plunger pump, tightened and sealed, and the air intake and exhaust of the plunger pump are completed through the outlet end 61b. The outlet end 61b of the plunger pump is connected to the common end 62a of the solenoid valve 62 by a pipeline; the air inlet end of the air filter 66 is connected and sealed to the muffler 67 by a threaded connection, and the air outlet end of the air filter 66 is connected to the "IN" port of the first one-way valve 64 by a pipeline; the "OUT" port of the first one-way valve 64 is connected to one of the ports of the three-way connector 63 by a pipeline, and one of the other two ports of the three-way connector 63 is connected to the normally open end 62b of the solenoid valve 62 by a pipeline, and the other port is connected to the "IN" port of the second one-way valve 65 by a pipeline; the normally closed end 62c of the solenoid valve 62 is connected to the pressure end 2128 of the probe assembly through a pipeline, and then connected to the probe 100, so that the entire air pressure source assembly is connected to the probe 100.

[0036] The air filter 66 has a muffler 67 connected to the air inlet end to reduce the noise of the air path system of the entire operating cell. The air filter 66 has an exhaust end connected to the "IN" port of the first one-way valve 64, which prevents the gas in the entire air path system from flowing back to the air filter 66; the second one-way valve 65 is used to exhaust the air in the plunger pump 61 to ensure that there is no unfiltered air in the plunger pump 61.

[0037] Before operation, the common end 62a and the normally open end 62b of the solenoid valve 62 are first connected, the normally closed end 62c is closed, and the plunger 61c first moves upward to the uppermost end, during which the residual gas in the plunger pump 61 is exhausted through the three-way joint 63 and the second one-way valve 65; then the plunger 61c moves downward to the middle position of the plunger pump 61, during which the air is filtered by the air filter 66 and enters the plunger pump 61 through the first one-way valve 64 and the three-way joint 63. The initial stop position of the plunger 61c is set at the middle of the plunger pump, and it can move downward to provide negative air pressure, and can also move upward to provide positive air pressure.

[0038] In some embodiments, Fig. 9 As shown, the plunger pump 61 provides positive or negative air pressure to the probe 100 in the form of pulses, and the plunger 61c moves intermittently within a single working stroke. When the plunger 61c moves intermittently upward, each movement distance is d1, and multiple positive air pressure pulses can be generated, the air pressure value of the positive air pressure pulse is P1, and the pulse period is T1; when the plunger 61c moves intermittently downward, each movement distance is d2, and multiple negative air pressure pulses can be generated, the air pressure value of the negative air pressure pulse is P2, and the pulse period is T2.

[0039] Fig. 9The origin O in the figure indicates that the plunger 61c is in the initial position, and the air pressure value in the plunger is close to the atmospheric pressure. Generally speaking, the pulse air pressure value is selected to be -10Kpa-10Kpa, and the period is less than 1s, preferably 0.5s-1s. The air pressure value and period of the pulse are adjustable to meet different practical needs.

[0040] The following uses cell extraction as an example to introduce the working principle of the air pressure source component: The solenoid valve 62 is energized, the normally open end 62b is closed, and the normally closed end 62c is opened, so that the plunger pump 61 is connected to the probe 100, and the plunger 61c in the plunger pump 61 is controlled to move downward. At this time, the plunger pump 61 provides a negative air pressure pulse to the probe 100, and the probe 100 can suck the cells in the culture dish. If a single pulse cannot suck the cells into the probe 100, multiple pulses can be sent continuously until the cells are sucked in. During the process of the probe 100 sucking the cells, when the internal and external air pressures are balanced, the cells no longer move, so they can be kept in the probe 100.

[0041] After the cells are sucked in, if the plunger 61c has not moved to the limit position, it can continue to move to provide pulses to suck in more cells.

[0042] When the plunger 61c moves downward to the limit position, the normally closed end 62c of the solenoid valve 62 is closed and the normally open end 62b is connected, and the plunger 61c moves upward to reset. During the reset process, the gas in the plunger pump 61 is discharged through the three-way connector 63 and the second one-way valve 65. After the plunger 61c is reset, the normally open end 62b is closed and the normally closed end 62c is opened, and cells can be continuously sucked in. The reciprocating operation can be repeated to suck more cells at different sites into the probe 100 in batches.

[0043] After the cells are sucked in, the plunger 61c is reset first (or not reset), and the plunger 61c in the plunger pump 61 is controlled to move upward, and a positive air pressure pulse is provided to the probe 100 at this time, and the probe 100 spits out the cells into the culture dish. Since the positive air pressure is also provided in the form of pulses, the cells can be spewed out one by one until all of them are spewed out. The working process is opposite to the process of sucking cells, and will not be repeated here.

[0044] By using positive or negative air pressure pulses, at most one cell can be ejected or sucked in each operation. Providing a negative air pressure pulse may prevent the cell from entering the probe due to insufficient suction. By continuously outputting multiple pulses, the cell can eventually enter the probe 100. The ejection of cells is similar to this.

[0045] Since the probe has a large space inside, it can temporarily store more cells. The probe 100 can continuously suck in multiple cells and then spit out the cells at once, which significantly improves the operation efficiency. In addition, the pulse probe 100 can spit out cells one by one, so that multiple cells are distributed in different positions, eliminating the redistribution operation.

[0046] It can be seen that the solenoid valve 62 serves as a pressure balancing device for the entire system. After the normally open end 62b is closed and the normally closed end 62c is opened, the plunger pump 61 does not work, and the gas path system connecting the probe 100 and the plunger pump 61 can maintain pressure balance, and the extracted cells can be completely preserved in the probe 100. The plunger 61c can then move again to generate new pulses. After the normally open end 62b is opened and the normally closed end 62c is closed, since the pressure has been balanced at this time, the plunger pump 61 performs a reset operation, which does not affect the cells preserved in the probe 100.

[0047] Other details of the single-cell manipulation device can be implemented in combination with existing technologies and will not be described in detail here.

[0048] The technical features of the above-mentioned embodiments can be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification. When the technical features in different embodiments are embodied in the same figure, it can be regarded that the figure also discloses the combination examples of the various embodiments involved.

[0049] The above-mentioned embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent application. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application.

Claims

1. A microscopic imaging component, used for imaging the stage, characterized in that: Includes: A sliding pair, defining a sliding direction relative to the stage, the sliding pair comprising a sliding member and a sliding seat; A lens seat is provided with a plurality of lenses, and the lens seat is rotatably arranged on the sliding member; In the sliding direction, the mirror mount has a first position close to the stage and a second position opposite to the stage; in the rotating direction, the mirror mount has a plurality of orientations, and in at least one orientation, one of the lenses is aligned with the stage, and in the first position, the lens is focused on the sample on the stage; An image detection unit is located below the lens base, and the lens imaging path runs through the lens base and forms an image through the image detection unit; A first driving device, comprising a first power source and a linkage member, wherein the linkage member drives the mirror holder to enter the first position or the second position under the action of the first power source; The second driving device includes a second power source, a first coupling member linked to the second power source, and a second coupling member arranged on the mirror base. When the mirror base enters the second position, the first coupling member and the second coupling member are coupled and the second power source can drive the mirror base to change the orientation. When the mirror base leaves the second position, the first coupling member and the second coupling member are separated.

2. The microscopic imaging assembly according to claim 1, characterized in that: The first driving device also includes a driving track arranged in the same direction as the sliding pair, and the linkage member includes a base moving on the driving track and a driving arm extending from the base toward the mirror seat, and the driving arm is used to drive the mirror seat into the first position.

3. The microscopic imaging assembly according to claim 2, characterized in that: The linkage member and the mirror base are unidirectionally transmitted, the mirror base is lifted by the linkage member to enter the first position, and is driven by gravity to fall back to the second position.

4. The microscopic imaging assembly according to claim 1, characterized in that: The mirror seat is rotatably arranged on the sliding member via a rotating shaft. The rotating shaft passes through the sliding pair and the second coupling member is arranged on the end thereof.

5. The microscopic imaging assembly according to claim 4, characterized in that: In the sliding direction of the sliding pair, an open mating groove is provided on the upper portion of the first coupling member. When the mirror mount enters the second position, at least a portion of the second coupling member enters the mating groove to achieve circumferential coupling between the second power source and the rotating shaft. When the mirror mount exits the second position, the second coupling member exits the mating groove to achieve separation.

6. The microscopic imaging assembly according to claim 4, characterized in that: The first coupling member is linked to a circumferential trigger member, the second driving device further includes a detector responsive to the circumferential trigger member, and the second power source is controlled by the detector.

7. The microscopic imaging assembly according to claim 1, characterized in that: The sliding pair is provided with two sliding pairs arranged side by side to enclose a receiving area, the mirror seat is located in the receiving area and connected to the sliding members of each sliding pair, and the first driving device and the second driving device are respectively located on both sides of the receiving area.

8. The microscopic imaging assembly according to claim 7, characterized in that: The mirror seat is rotatably mounted on the mirror frame via a rotating shaft. The mirror frame is U-shaped and both sides of the U-shape are respectively connected to the sliding members of each sliding pair. The rotating shaft is set through both sides of the U-shaped opening of the mirror frame and the mirror seat is accommodated inside the U-shape.

9. The microscopic imaging assembly according to claim 8, characterized in that: The lens holder is provided with a plurality of mounting surfaces, each mounting surface is used to mount the lens and a limiting portion extending outward, the frame is provided with a blocking member for cooperating with the limiting portion, and when the lens holder is adjusted to a preset orientation, the limiting portion and the blocking member abut against each other to accurately position the orientation of the lens holder.

10. A single cell manipulation device, characterized in that: include: A stage for fixing the container carrying the sample; A probe assembly, comprising a probe and a holder for holding the probe, wherein the probe is an elongated hollow structure having a front end and a rear end opposite to each other, wherein the front end of the probe extends to the container and the rear end of the probe is inserted into the holder when in use; a control assembly, including a moving platform for moving the probe assembly in space to adjust the position of the probe; A microscopic imaging assembly, which is the microscopic imaging assembly according to any one of claims 1 to 9 and provides a window for positioning the probe; An air pressure source assembly is connected to the probe via the holder to provide positive and / or negative air pressure to the probe.

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