Molecular biology experiment sample grinder
By designing adjustable brackets and lockers, combined with the use of compressors, the problem that the grinder tray in the prior art cannot adapt to grinding pipes of different sizes is solved, effectively positioning and use of grinding pipes of different sizes is achieved, and the applicability and stability of the equipment are improved.
Patent Information
- Application Number
- CN202510054593.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-14
- Publication Date
- 2025-04-15
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The tray of existing molecular biology experimental sample grinders has fixed groove sizes, which cannot adapt to grinding tubes of different sizes, resulting in the inability to better apply various experimental samples.
A molecular biology experimental sample grinder was designed, using an adjustable bracket and locker, which can adapt to grinding tubes of different diameters, and re-compression positioning of the grinding tubes is achieved through the presser.
Effective positioning and use of grinding pipes of different sizes is achieved, the problem of loosening and disengagement of grinding pipes during oscillation is avoided, and the applicability and stability of the equipment are improved.
Smart Images
Figure CN119819443A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of experimental sample grinders, in particular to a molecular biology experimental sample grinder. Background Art
[0002] Molecular biology experiment sample grinder is an instrument used to grind molecular biology experiment samples, such as tissue grinders used for dry grinding, wet grinding, cryo-grinding, cell disruption and DNA / RNA extraction. The grinder allows the grinding beads to oscillate at high speed in the sample tube to achieve sample crushing, mixing, homogenization and cell disruption, etc. It can quickly crush and homogenize hard, soft, elastic and other samples.
[0003] The grinding instrument includes a grinding instrument body and a body cover. There is an oscillation column in the placement cavity on the top of the body, and a grinding tube placement tray is arranged on the rotation, and a placement slot is provided on the tray. A compressor screwed to the oscillation column is arranged on the tray. During operation, the sample is placed in a grinding tube filled with a buffer solution, and then 3-5 zirconia beads / stainless steel beads / ceramic beads / glass beads are placed in the grinding tube. After the grinding tube cover is closed, the grinding tube is placed in the tissue grinder tube placement slot in a balanced manner, and then the compressor is screwed on the oscillation column and pressed on the top of the grinding tube in the placement slot, and then the protective cover is covered and locked by the lock, and then the "Set" button on the surface of the body is pressed to select the vibration intensity, running time, pause time and number of cycles of the grinding and homogenization program. After setting the parameters, press the "Run" button to start grinding.
[0004] The size of the placement slots on the tray in the above-mentioned grinding instrument is fixed, but the size of the grinding material and the corresponding grinding tube used is not fixed, so the original tray cannot be better adapted. For this reason, we propose a molecular biology experiment sample grinder to solve the above problem. Summary of the invention
[0005] The purpose of the present invention is to solve the shortcomings in the prior art and to propose a molecular biology experiment sample grinder.
[0006] In order to achieve the above object, the present invention adopts the following technical solutions: A molecular biology experiment sample grinder, comprising a grinder, a mounting cavity is provided on the top of the grinder, a protective cover is provided on the top of the grinder, and an oscillating column is provided in the mounting cavity, wherein the oscillating column comprises a connecting portion A, a connecting portion B, a connecting portion C and a connecting portion D; The connection part B is sleeved with a bracket, and the bracket includes a collar A and a supporting ring. The collar A is sleeved on the outside of the connection part B and overlapped on the top of the connection part A. A plurality of connecting blocks arranged in a circumference are connected between the outer ring of the collar A and the inner ring of the supporting ring. The supporting ring is provided with a plurality of placement holes, and an annular connecting groove is provided on the supporting ring and is located at the outer ring position of the placement hole. The supporting ring is connected with a locking device; The connecting portion B is provided with a locking cover on the outside and is pressed tightly on the top of the collar A.
[0007] As preferred in the present invention, the locking device includes an annular connecting portion connected to the annular connecting groove in a threaded manner, the annular connecting portion and the placement hole are generally located on the same axial line, and the annular connecting portion has a plurality of circumferentially distributed clamping portions, the relative surfaces of the clamping portions are all provided with clamping rubbers, and the same locking ring A is provided on the outer sides of the plurality of clamping portions.
[0008] When in use, the bottom end of the grinding tube is placed downwardly into the hole between the multiple clamping parts, so that the top end of the grinding tube is located at the top of the multiple clamping parts. Then the staff rotates the locking ring A upward from bottom to top. During the upward rotation of the locking ring A, the multiple clamping parts are clamped and squeezed on the surface of the grinding tube through the clamping rubber, thereby positioning the grinding tube in the placement hole. Then the protective cover is covered on the top of the grinder and the grinder is started. In this way, the grinder drives the bracket to work through the oscillation column, and further drives the grinding tube located in the placement hole and locked by the locker to oscillate at high speed. In this way, the grinding beads oscillate at high speed in the sample tube to achieve sample crushing, mixing, homogenization and cell disruption, etc., and can quickly crush and homogenize hard, soft, elastic and other samples. In addition, the use of the locker can also clamp and position grinding tubes of different diameters.
[0009] As a preferred embodiment of the present invention, the connection part B is provided with a circle of positioning grooves A along the outside, and the ring A is provided with a circle of positioning protrusions A along the inner circle, and the positioning protrusions A match the positioning grooves A. When the ring A is sleeved on the outside of the connection part B and located at the top of the connection part A, and the positioning protrusions A at the inner circle position of the ring A are located in the positioning grooves A, the ring A cannot rotate on the connection part B.
[0010] As a preferred embodiment of the present invention, the locking cover includes a ring cover sleeved on the connecting part D, the ring cover is screwed on the connecting part B by means of threads, wherein the connecting part C and the top part of the connecting part B are both covered by the ring cover, and a damping member is provided on the inner ring of the ring cover, the damping member is located at the inner ring position of the ring cover and close to the inner top, and the damping member includes a damping ring A, which is sleeved on the connecting part C; when the ring cover is threadedly connected to the connecting part B, the damping ring A in the ring cover is located outside the connecting part C, and then the bolts are penetrated and screwed into the ring cover and the damping ring A is squeezed on the surface of the connecting part C, thereby further locking the ring cover to prevent the ring cover from loosening and detaching, so as to achieve the positioning and tightening of the ring cover on the sleeve ring A.
[0011] As a preferred embodiment of the present invention, the connection part C is provided with a circle of positioning grooves B along the outside, and the inner ring of the damping ring A is provided with a plurality of positioning protrusions B, which are distributed circumferentially along the inner ring of the damping ring A, and the positioning protrusions B match the positioning grooves B. When the damping ring A is sleeved on the outside of the connection part C, the positioning protrusions B of the inner ring of the damping ring A match the positioning grooves B, so that the damping ring A cannot rotate outside the connection part C, so that when the damping ring A is screwed into the ring cover by bolts and squeezed on the surface of the connection part C, the damping ring A will not be squeezed and slipped outside the connection part C.
[0012] As preferred in the present invention, the connection part D is externally connected with a clamp, and the clamp includes a locking ring B, which is sleeved on the outside of the connection part D in a threaded manner, and a clamping ring is provided at the middle section of the inner ring of the locking ring B, and the locking ring B is externally rotatably connected with a conical ring edge, and the outer ring of the conical ring edge is connected with a plurality of clamping arms, and the plurality of clamping arms are distributed in a circle, and a clamping block is fixed at one end of the clamping arm away from the conical ring edge. When in use, the clamping ring is sleeved on the outside of the connection part D and located on the top of the ring cover, and then the locking ring B is screwed on the outside of the connection part D in a threaded manner, and the connection part D is further screwed downward and the clamping arm is driven to press downward through the conical ring edge, and further the clamping arm drives the clamping block to press downward on the top of the grinding tube located in the placement hole and locked by the clamp, thereby realizing re-compression and positioning of the grinding tube.
[0013] As preferred in the present invention, a placement hole is provided on the top of the connecting portion D, and a placement seat is provided at the bottom of the placement hole. A plurality of embedding grooves with increasingly shallow depths are provided in sequence from the middle to the outside on the top of the placement seat. The embedding grooves of different depths are used to match the bottom ends of grinding tubes / centrifuge tubes of different diameters. A positioning cover is screwed on the top of the connecting portion D in a threaded manner, and a clamping portion A is provided at the middle position of the bottom end of the positioning cover. A clamping portion B is also provided in the placement hole. When in use, the grinding tube / centrifuge tube can be placed in the placement hole, and the bottom end of the grinding tube / centrifuge tube can be embedded in the corresponding embedding groove. Then, a clamping portion B of different thicknesses selected according to the height of the grinding tube / centrifuge tube can be placed in the placement hole and pressed on the top of the grinding tube / centrifuge tube. Then, the positioning cover is screwed on the top of the connecting portion D in a threaded manner, and the clamping portion A is pressed on the clamping portion B to further clamp the top of the grinding tube / centrifuge tube, so that the top of the grinding tube / centrifuge tube is positioned in the placement hole.
[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention can not only hold and position grinding tubes of different diameters, but also hold and position grinding tubes in placement holes through the setting of the locker. In addition, the grinding tube can be compressed again after using the compressor. In this way, grinding together can be suitable for grinding tubes of different sizes, and grinding tubes of different sizes can be better positioned to prevent the grinding tubes from loosening and detaching during vibration.
[0015] 2. In the present invention, a grinding tube or a centrifuge tube can be arranged in the placement hole, so that grinding and centrifugation operations can be performed, and grinding tubes or centrifuge tubes with larger diameters can be adapted for use. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 An axial view of a molecular biology experiment sample grinder proposed by the present invention; Figure 2 for Figure 1 Structural diagram of the middle oscillation column, bracket, locker and locking cover; Figure 3 for Figure 2 Exploded view of the middle locking cover and the oscillation column; Figure 4 for Figure 2 Exploded diagram of the middle bracket and the shock column; Figure 5 The following are the disassembled diagrams of the 4 types of locks and brackets; Figure 6 This is a disassembled diagram of the mechanism of 5 types of locking devices; Figure 7 Schematic diagram of the clamp in 1 being connected to the connecting portion D and pressed on the locking device; Figure 8FIG. 7 is a disassembled view of the pressing device and the connecting portion D; Fig. 9 8 is a schematic diagram of the structure of the compactor when viewed from above; Fig.10 1 is a schematic diagram of the connection between the positioning cover and the connecting portion D; Fig.11 It is a structural schematic diagram of the positioning cover, the placement hole, the clamping part B, and the placement seat 10.
[0017] In the figure: 1, oscillation column; 11, connection part A; 12, connection part B; 121, positioning groove A; 13, connection part C; 131, positioning groove B; 14, connection part D; 141, placement hole; 2, bracket; 21, collar A; 211, positioning convex A; 22, connection block; 23, support ring; 24, placement hole; 241, annular connection groove; 3, locker; 31, annular connection part; 32, holding part; 3 3. Holding rubber; 34. Locking ring A; 4. Locking cover; 41. Damping element; 411. Damping ring A; 412. Positioning cam B; 42. Ring cover; 5. Presser; 51. Locking ring B; 52. Conical ring edge; 53. Pressing arm; 54. Pressing block; 55. Pressing ring; 6. Placement seat; 7. Positioning cover; 71. Pressing part A; 72. Pressing part B; 8. Grinder; 81. Installation cavity; 82. Protective cover. DETAILED DESCRIPTION
[0018] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0019] In the description of the present invention, “plurality” means two or more than two, unless otherwise clearly and specifically defined.
[0020] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "mounted / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0021] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.
[0022] Reference Figure 1-11 A molecular biology experimental sample grinder includes a grinder 8, a mounting cavity 81 is provided on the top of the grinder 8, and a protective cover 82 is provided on the top of the grinder 8, and an oscillation column 1 is arranged in the mounting cavity 81, the oscillation column 1 includes a connection part A11, a connection part B12, a connection part C13 and a connection part D14, the connection part A11 is connected to the output shaft of a driving device in the grinder 8, such as the output shaft of a driving motor, the connection part B12 is sleeved with a bracket 2, the bracket 2 includes a collar A21 and a supporting ring 23, the collar A21 is sleeved on the outside of the connection part B12 and overlapped on the top of the connection part A11, and a plurality of collars are connected between the outer ring of the collar A21 and the inner ring of the supporting ring 23 and are in a The connecting block 22 is arranged in a circle, and a plurality of placement holes 24 are provided on the supporting ring 23. An annular connecting groove 241 is provided on the supporting ring 23 and is located at the outer circle position of the placement hole 24. A locking cover 4 is sleeved on the outside of the connecting part B12 and is pressed on the top of the sleeve A21. The supporting ring 23 is connected with a locking device 3. The locking device 3 includes an annular connecting part 31 connected to the annular connecting groove 241 in a threaded manner. The annular connecting part 31 and the placement hole 24 are generally located on the same axial line, and the annular connecting part 31 has a plurality of circumferentially distributed clamping parts 32. The relative surfaces of the clamping parts 32 are all provided with clamping rubbers 33, and the outer surfaces of the plurality of clamping parts 32 are sleeved with the same locking ring A34.
[0023] When in use, the bottom end of the grinding tube is placed downwardly in the hole 24 between the multiple clamping parts 32, so that the top end of the grinding tube is located at the top position of the multiple clamping parts 32, and then the staff rotates the locking ring A34 upward from bottom to top. During the upward rotation of the locking ring A34, the multiple clamping parts 32 are clamped and squeezed on the surface of the grinding tube through the clamping rubber 33, thereby positioning the grinding tube in the placement hole 24, and then the protective cover 82 is covered on the top of the grinder 8, and the grinder 8 is started. In this way, the grinder 8 drives the bracket 2 to work through the oscillation column 1, and further drives the grinding tube located in the placement hole 24 and locked by the locker 3 to oscillate at high speed. In this way, the grinding beads oscillate at high speed in the sample tube to achieve sample crushing, mixing, homogenization and cell disruption, etc., and can quickly crush and homogenize hard, soft, elastic and other samples. In addition, the use of the locker 3 can also clamp and position grinding tubes of different diameters.
[0024] Reference Figure 1-11A molecular biology experimental sample grinder, a connection part B12 is provided with a circle of positioning grooves A121 along the outside, a ring A21 is provided with a circle of positioning protrusions A211 along the inner circle, the positioning protrusions A211 match the positioning grooves A121, when the ring A21 is sleeved on the outside of the connection part B12 and located at the top of the connection part A11, and the positioning protrusions A211 at the inner circle position of the ring A21 are located in the positioning grooves A121, the ring A21 cannot rotate on the connection part B12.
[0025] Reference Figure 1-11 , a molecular biology experimental sample grinder, the locking cover 4 includes a ring cover 42 sleeved on the connection part D14, the ring cover 42 is screwed on the connection part B12 by threads, wherein the connection part C13 and the top part of the connection part B are both covered by the ring cover 42, and the inner circle of the ring cover 42 is provided with a damping member 41, the damping member 41 is located at the inner circle position of the ring cover 42 and close to the inner top, and the damping member 41 includes a damping ring A411, and the damping ring A411 is sleeved on the connection part C13; when the ring cover 42 is connected to the connection part B12 by threads, the damping ring A411 in the ring cover 42 is located outside the connection part C13, and then the bolts are penetrated and screwed into the ring cover 42 and the damping ring A411 is squeezed on the surface of the connection part C13, so that the ring cover 42 is further locked to prevent the ring cover 42 from loosening and detaching, so as to realize the positioning and pressing of the ring A21 by the ring cover 42.
[0026] Reference Figure 1-11 A molecular biology experimental sample grinder, a connection part C13 is provided with a circle of positioning grooves B131 along the outside, a damping ring A411 is provided with a plurality of positioning protrusions B412 on the inner circle, the positioning protrusions B412 are distributed in a circle along the inner circle of the damping ring A411, the positioning protrusions B412 match with the positioning grooves B131, when the damping ring A411 is sleeved on the outside of the connection part C13, the positioning protrusions B412 of the inner circle of the damping ring A411 match with the positioning grooves B131, so that the damping ring A411 cannot rotate outside the connection part C13, so that when the damping ring A411 is screwed into the ring cover 42 by bolts and squeezed on the surface of the connection part C13, the damping ring A411 will not be squeezed and slipped outside the connection part C13.
[0027] Reference Figure 1-11A molecular biology experiment sample grinder, a connection portion D14 is externally connected to a clamp 5, the clamp 5 comprises a locking ring B51, the locking ring B51 is sleeved on the outside of the connection portion D14 in a threaded connection manner, and a clamp 55 is provided at the middle section of the inner ring of the locking ring B51, the locking ring B51 is externally rotatably connected to a conical ring edge 52, and the outer ring of the conical ring edge 52 is connected to a plurality of clamp arms 53, the plurality of clamp arms 53 are circumferentially distributed, and one side of the clamp arm 53 away from the conical ring edge 52 is provided with a clamp. A clamping block 54 is fixed at the end. When in use, the pressing ring 55 is put on the outside of the connecting part D14 and is located on the top of the ring cover 42, and then the locking ring B51 is screwed on the outside of the connecting part D14 in a threaded connection manner. The connecting part D14 is further screwed downward and the clamping arm 53 is driven to press downward through the conical ring edge 52. The clamping arm 53 further drives the clamping block 54 to press downward on the top of the grinding tube located in the placement hole 24 and locked by the locker 3, thereby realizing the re-compression and positioning of the grinding tube.
[0028] Reference Figure 1-11 A molecular biology experimental sample grinder, a connection portion D14 is provided with a placement hole 141 at the top, and a placement seat 6 is provided at the bottom of the placement hole 141, and a plurality of embedding grooves with increasingly shallow depths are provided on the top of the placement seat 6 from the middle to the outside, and the embedding grooves of different depths are used to match the bottom ends of grinding tubes / centrifuge tubes of different diameters, a positioning cover 7 is screwed on the top of the connection portion D14 in a threaded manner, and a clamping portion A71 is provided at the middle position of the bottom end of the positioning cover 7, and a clamping portion B72 is also provided in the placement hole 141, When in use, the grinding tube / centrifuge tube can be placed in the placement hole 141, and the bottom end of the grinding tube / centrifuge tube can be embedded in the corresponding embedding groove. Then, the clamping part B72 of different thicknesses selected according to the height of the grinding tube / centrifuge tube can be placed in the placement hole 141 and pressed on the top of the grinding tube / centrifuge tube. Then, the positioning cover 7 is screwed on the top of the connecting part D14 in a threaded connection manner, and the clamping part A71 is pressed on the clamping part B72 to further clamp the top of the grinding tube / centrifuge tube, so that the top of the grinding tube / centrifuge tube is positioned in the placement hole 141.
[0029] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.
[0030] In the description of the present invention, it is necessary to understand that the terms indicating orientation or positional relationship are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.
Claims
1. A molecular biology experimental sample grinder, comprising a grinder (8), wherein a mounting cavity (81) is provided on the top of the grinder (8), and a protective cover (82) is provided on the top of the grinder (8), characterized in that: It also includes an oscillation column (1) arranged in the installation cavity (81), the oscillation column (1) comprising a connection portion A (11), a connection portion B (12), a connection portion C (13) and a connection portion D (14); The connection portion B (12) is provided with a bracket (2), the bracket (2) comprising a collar A (21) and a supporting ring (23), the collar A (21) being provided outside the connection portion B (12) and overlapping the top of the connection portion A (11), a plurality of connecting blocks (22) being connected between the outer ring of the collar A (21) and the inner ring of the supporting ring (23) and being arranged in a circumferential manner, a plurality of placement holes (24) being provided on the supporting ring (23), and an annular connecting groove (241) being provided on the supporting ring (23) and being located at the outer ring position of the placement hole (24), and a locking device (3) being connected to the supporting ring (23); The connecting portion B (12) is provided with a locking cover (4) on the outside and is pressed against the top of the collar A (21).
2. A molecular biology experiment sample grinder according to claim 1, characterized in that: The locking device (3) comprises an annular connecting portion (31) connected to the annular connecting groove (241) in a threaded manner, the annular connecting portion (31) and the placement hole (24) are generally located on the same axial center line, and the annular connecting portion (31) has a plurality of circumferentially distributed clamping portions (32), the relative surfaces of the clamping portions (32) are all provided with clamping rubbers (33), and the outer surfaces of the plurality of clamping portions (32) are provided with a same locking ring A (34).
3. A molecular biology experiment sample grinder according to claim 1, characterized in that: The connection portion B (12) is provided with a circle of positioning grooves A (121) along the outside, and the collar A (21) is provided with a circle of positioning protrusions A (211) along the inner circle, wherein the positioning protrusions A (211) match the positioning grooves A (121).
4. A molecular biology experiment sample grinder according to claim 1, characterized in that: The locking cover (4) comprises a ring cover (42) sleeved on the connecting portion D (14), the ring cover (42) being screwed onto the connecting portion B (12) via a thread, a damping member (41) being provided on the inner ring of the ring cover (42), and the damping member (41) comprising a damping ring A (411), the damping ring A (411) being sleeved on the connecting portion C (13).
5. The molecular biology experiment sample grinder according to claim 1, characterized in that: The connection portion C (13) is provided with a circle of positioning grooves B (131) along the outside, and the inner circle of the damping ring A (411) is provided with a plurality of positioning protrusions B (412). The positioning protrusions B (412) are distributed in a circular pattern along the inner circle of the damping ring A (411), and the positioning protrusions B (412) match the positioning grooves B (131).
6. A molecular biology experiment sample grinder according to claim 1, characterized in that: The connection portion D (14) is externally connected to a clamp (5), the clamp (5) comprising a locking ring B (51), the locking ring B (51) being sleeved on the connection portion D (14) in a threaded manner, and a clamping ring (55) is provided at the middle of the inner ring of the locking ring B (51), the locking ring B (51) is externally rotatably connected to a conical ring edge (52), and the outer ring of the conical ring edge (52) is connected to a plurality of clamping arms (53), the plurality of clamping arms (53) being distributed in a circular pattern, and a clamping block (54) is fixed to one end of the clamping arm (53) away from the conical ring edge (52).
7. A molecular biology experiment sample grinder according to claim 1, characterized in that: The top of the connecting portion D (14) is provided with a placement hole (141), and the bottom of the placement hole (141) is provided with a placement seat (6), and the top of the placement seat (6) is provided with a plurality of placement grooves with increasingly shallow depths in sequence from the middle to the outside. The top of the connecting portion D (14) is screwed with a positioning cover (7) in a threaded manner, and a clamping portion A (71) is provided at the middle position of the bottom end of the positioning cover (7), and a clamping portion B (72) is also provided in the placement hole (141).
8. The molecular biology experiment sample grinder according to claim 1, characterized in that: The connection part A (11), the connection part B (12), the connection part C (13) and the connection part D (14) are integrated from bottom to top.