TaqMan-MGB multiple fluorescent quantitative PCR (polymerase chain reaction) primer probe group, kit and method for detecting three pathogenic eimeria of goat

Abstract

The invention relates to the technical field of parasite detection, in particular to a TaqMan-MGB multiple fluorescent quantitative PCR (polymerase chain reaction) primer group, a probe, a kit and a method for detecting three pathogenic eimeria of goats. The invention relates to a TaqMan-MGB multiplex fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a probe for detecting Eimeria klebsii, Eimeria adamsii and Eimeria elegans, and a TaqMan-MGB multiplex fluorescent quantitative PCR method for detecting the Eimeria klebsii, the Eimeria adamsii and the Eimeria elegans is established on the basis of the primer group and the probe, so that the TaqMan-MGB multiplex fluorescent quantitative PCR primer group and the probe can be used for detecting the Eimeria klebsii, the Eimeria adamsii and the Eimeria elegans, the Eimeria adamsii and the Eimeria elegans. According to the primer group, the probe and the TaqMan-MGB multiple fluorescent quantitative PCR method disclosed by the invention, the mixed infection and single infection conditions of the Eimeria kirsuki, the Eimeria adamsii and the Eimeria delbrueckii in a clinical sample to be detected can be determined through one TaqMan-MGB multiple fluorescent quantitative PCR reaction; compared with common PCR and nano PCR methods, the method has the advantages of good repeatability, high sensitivity, strong specificity, simple operation and the like.

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