Traditional Chinese medicine active coating based on temperature and pH value double-response hydrogel and preparation method

Abstract

The invention relates to the technical field of traditional Chinese medicine active coatings, and provides a traditional Chinese medicine active coating based on temperature and pH value double-response hydrogel and a preparation method of the traditional Chinese medicine active coating. The invention relates to an anti-aging agent, which is prepared from the following components in parts by weight: 10 to 20 parts of chitosan, 25 to 50 parts of hyaluronic acid, 5 to 15 parts of sodium alginate, 30 to 80 parts of poly (N-isopropylacrylamide), 10 to 25 parts of polyacrylic acid, 5 to 10 parts of polyethylene glycol, 10 to 20 parts of nano cellulose and 20 to 40 parts of baicalein. By means of the technical scheme, the problem that the sterilization effect on filtered air is not ideal due to the fact that an existing air filtering device is not provided with a traditional Chinese medicine active coating is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine active coatings, and in particular to a traditional Chinese medicine active coating based on a temperature and pH value dual-responsive hydrogel and a preparation method thereof. Background Art

[0002] The TCM active coating optimizes the efficacy and safety of the active ingredients of TCM by adding a layer of material to the surface. This technology combines traditional Chinese medicine with modern nanotechnology to improve the release characteristics of the drug, making it more efficient in the body. In particular, the TCM active coating is used in industrial plants where gas with bacteria is produced.

[0003] Although existing industrial plants are equipped with air filtration devices, these devices only filter harmful gases in the air and cannot kill bacteria in the air. If the air cannot be disinfected in time, the bacterial content in the air in some internal circulation plants will only increase, and in serious cases it will even threaten the health of operators.

[0004] Therefore, a Chinese medicine coating is continued to be used on the filter net to sterilize the filtered space. Summary of the invention

[0005] The present invention proposes a traditional Chinese medicine active coating based on a temperature and pH value dual-responsive hydrogel and a preparation method, which solves the problem that the existing air filter device has an unsatisfactory sterilization effect on filtered air due to the lack of a traditional Chinese medicine active coating.

[0006] The technical scheme of the present invention is as follows: a traditional Chinese medicine active coating based on temperature and pH value dual-responsive hydrogel comprises, by weight: 10-20 parts of chitosan, 25-50 parts of hyaluronic acid, 5-15 parts of sodium alginate, 30-80 parts of poly N-isopropylacrylamide, 10-25 parts of polyacrylic acid, 5-10 parts of polyethylene glycol, 10-20 parts of nanocellulose, and 20-40 parts of baicalein.

[0007] As a further technical solution, the invention comprises, by weight: 15 parts of chitosan, 35 parts of hyaluronic acid, 10 parts of sodium alginate, 55 parts of poly (N-isopropylacrylamide), 15 parts of polyacrylic acid, 8 parts of polyethylene glycol, 15 parts of nanocellulose and 30 parts of baicalein.

[0008] As a further technical solution, the invention further comprises, by weight: 20-40 parts of fluorescent markers.

[0009] As a further technical solution, the invention comprises, by weight: 15 parts of chitosan, 35 parts of hyaluronic acid, 10 parts of sodium alginate, 55 parts of poly (N-isopropylacrylamide), 15 parts of polyacrylic acid, 8 parts of polyethylene glycol, 15 parts of nanocellulose, 30 parts of baicalein and 30 parts of fluorescent marker.

[0010] The present invention also provides a method for preparing a traditional Chinese medicine active coating based on a temperature and pH value dual-responsive hydrogel, comprising the following steps:

[0011] Step 1: Add chitosan, hyaluronic acid and sodium alginate to distilled water in proportion and mix. After fully mixing, add 0.5% hydrogen peroxide liquid, the liquid accounts for 1%-2% of the total mixture. After fully mixing, let stand for 1-2 hours;

[0012] Step 2: After the standing time is completed, poly (N-isopropylacrylamide), polyacrylic acid, polyethylene glycol and nanocellulose are added in proportion, mixed thoroughly and allowed to stand for 20-30 minutes;

[0013] Step 3, adjusting the pH value of the mixed solution to 4-5 and the temperature value to 22-28°C, and then maintaining the pH value and temperature value for 0.5-1h;

[0014] Step 4: air-dry the mixture to remove excess liquid until the mixture becomes a semi-dry film;

[0015] Step 5: Apply the semi-dry film evenly on the surface of the base. No bubbles should appear during the application process. Apply it in layers until the required Chinese medicine active coating is formed.

[0016] As a further technical solution, in the step 1, chitosan, hyaluronic acid and sodium alginate are mixed by stirring, and bubbles are prevented from being mixed into the mixture during the mixing process.

[0017] As a further technical solution, in step 4, the wind speed and wind temperature of air drying can be adjusted, the wind speed is less than 1 m / s; the wind temperature is less than 50°C.

[0018] As a further technical solution, in step five, the number of layered coating is greater than three layers.

[0019] The invention has the following beneficial effects: chitosan is a polysaccharide with good hydrophilicity and biocompatibility; hyaluronic acid and sodium alginate are both inorganic high molecular polymers and are generally used for thickening hydrogels; poly N-isopropylacrylamide is a temperature-sensitive material and is used for cross-linking with baicalein to form a stable combination; polyacrylic acid forms a conjugate with baicalein to enhance the cross-linking ability; polyethylene glycol is used as a cross-linking agent to promote the cross-linking between poly N-isopropylacrylamide and baicalein; and nanocellulose can provide physical support to avoid ineffective release of drugs. DETAILED DESCRIPTION

[0020] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Example 1

[0022] This embodiment proposes a traditional Chinese medicine active coating based on temperature and pH dual-responsive hydrogel, which includes, by weight: 10 parts of chitosan, 25 parts of hyaluronic acid, 5 parts of sodium alginate, 30 parts of poly (N-isopropylacrylamide), 10 parts of polyacrylic acid, 5 parts of polyethylene glycol, 10 parts of nanocellulose, and 20 parts of baicalein.

[0023] The preparation method of the traditional Chinese medicine active coating comprises the following steps:

[0024] Step 1: Add chitosan, hyaluronic acid and sodium alginate to distilled water in proportion and mix. After fully mixing, add 0.5% hydrogen peroxide liquid, the liquid accounts for 1%-2% of the total mixture. After fully mixing, let stand for 1-2 hours;

[0025] Step 2: After the standing time is completed, poly (N-isopropylacrylamide), polyacrylic acid, polyethylene glycol and nanocellulose are added in proportion, mixed thoroughly and allowed to stand for 20-30 minutes;

[0026] Step 3, adjusting the pH value of the mixed solution to 4-5 and the temperature value to 22-28°C, and then maintaining the pH value and temperature value for 0.5-1h;

[0027] Step 4: air-dry the mixture to remove excess liquid until the mixture becomes a semi-dry film;

[0028] Step 5: Apply the semi-dry film evenly on the surface of the base. No bubbles should appear during the application process. Apply it in layers until the required Chinese medicine active coating is formed.

[0029] Example 2

[0030] This embodiment proposes a traditional Chinese medicine active coating based on temperature and pH dual-responsive hydrogel, which includes, by weight: 20 parts of chitosan, 50 parts of hyaluronic acid, 15 parts of sodium alginate, 80 parts of poly (N-isopropylacrylamide), 25 parts of polyacrylic acid, 10 parts of polyethylene glycol, 20 parts of nanocellulose, and 40 parts of baicalein.

[0031] The preparation method of the traditional Chinese medicine active coating comprises the following steps:

[0032] Step 1: Add chitosan, hyaluronic acid and sodium alginate to distilled water in proportion and mix. After fully mixing, add 0.5% hydrogen peroxide liquid, the liquid accounts for 1%-2% of the total mixture. After fully mixing, let stand for 1-2 hours;

[0033] Step 2: After the standing time is completed, poly (N-isopropylacrylamide), polyacrylic acid, polyethylene glycol and nanocellulose are added in proportion, mixed thoroughly and allowed to stand for 20-30 minutes;

[0034] Step 3, adjusting the pH value of the mixed solution to 4-5 and the temperature value to 22-28°C, and then maintaining the pH value and temperature value for 0.5-1h;

[0035] Step 4: air-dry the mixture to remove excess liquid until the mixture becomes a semi-dry film;

[0036] Step 5: Apply the semi-dry film evenly on the surface of the base. No bubbles should appear during the application process. Apply it in layers until the required Chinese medicine active coating is formed.

[0037] Example 3

[0038] This embodiment proposes a traditional Chinese medicine active coating based on temperature and pH dual-responsive hydrogel, which includes, by weight: 15 parts of chitosan, 35 parts of hyaluronic acid, 10 parts of sodium alginate, 55 parts of poly (N-isopropylacrylamide), 15 parts of polyacrylic acid, 8 parts of polyethylene glycol, 15 parts of nanocellulose, and 30 parts of baicalein.

[0039] The preparation method of the traditional Chinese medicine active coating comprises the following steps:

[0040] Step 1: Add chitosan, hyaluronic acid and sodium alginate to distilled water in proportion and mix. After fully mixing, add 0.5% hydrogen peroxide liquid, the liquid accounts for 1%-2% of the total mixture. After fully mixing, let stand for 1-2 hours;

[0041] Step 2: After the standing time is completed, poly (N-isopropylacrylamide), polyacrylic acid, polyethylene glycol and nanocellulose are added in proportion, mixed thoroughly and allowed to stand for 20-30 minutes;

[0042] Step 3, adjusting the pH value of the mixed solution to 4-5 and the temperature value to 22-28°C, and then maintaining the pH value and temperature value for 0.5-1h;

[0043] Step 4: air-dry the mixture to remove excess liquid until the mixture becomes a semi-dry film;

[0044] Step 5: Apply the semi-dry film evenly on the surface of the base. No bubbles should appear during the application process. Apply it in layers until the required Chinese medicine active coating is formed.

[0045] In Examples 1-3, since chitosan is a polysaccharide with good hydrophilicity and biocompatibility, it is well compatible with baicalin; hyaluronic acid and sodium alginate are both inorganic high molecular polymers and are usually used to thicken hydrogels, so they are also well compatible with baicalin; poly (N-isopropylacrylamide) is a thermosensitive material, which is used to cross-link with baicalin to form a stable combination, and polyacrylic acid will form a conjugate with baicalin to enhance the cross-linking ability; polyethylene glycol is used as a cross-linking agent to promote the cross-linking between poly (N-isopropylacrylamide) and baicalin; nanocellulose can provide physical support to avoid ineffective release of drugs, so nanocellulose is well compatible with baicalin.

[0046] Comparative Example 1

[0047] This embodiment proposes a traditional Chinese medicine active coating based on temperature and pH dual-responsive hydrogel, which includes, by weight: 15 parts of chitosan, 35 parts of hyaluronic acid, 10 parts of sodium alginate, 55 parts of poly (N-isopropylacrylamide), 15 parts of polyacrylic acid, 8 parts of polyethylene glycol, and 30 parts of baicalein.

[0048] The preparation method of the traditional Chinese medicine active coating comprises the following steps:

[0049] Step 1: Add chitosan, hyaluronic acid and sodium alginate to distilled water in proportion and mix. After fully mixing, add 0.5% hydrogen peroxide liquid, the liquid accounts for 1%-2% of the total mixture. After fully mixing, let stand for 1-2 hours;

[0050] Step 2: After the standing time is completed, poly (N-isopropylacrylamide), polyacrylic acid and polyethylene glycol are added in proportion, mixed thoroughly and allowed to stand for 20-30 minutes;

[0051] Step 3, adjusting the pH value of the mixed solution to 4-5 and the temperature value to 22-28°C, and then maintaining the pH value and temperature value for 0.5-1h;

[0052] Step 4: air-dry the mixture to remove excess liquid until the mixture becomes a semi-dry film;

[0053] Step 5: Apply the semi-dry film evenly on the surface of the base. No bubbles should appear during the application process. Apply it in layers until the required Chinese medicine active coating is formed.

[0054] Comparative Example 2

[0055] This embodiment proposes a traditional Chinese medicine active coating based on temperature and pH dual-responsive hydrogel, which includes, by weight: 15 parts of chitosan, 35 parts of hyaluronic acid, 10 parts of sodium alginate, 55 parts of poly (N-isopropylacrylamide), 8 parts of polyethylene glycol, 15 parts of nanocellulose, and 30 parts of baicalein.

[0056] The preparation method of the traditional Chinese medicine active coating comprises the following steps:

[0057] Step 1: Add chitosan, hyaluronic acid and sodium alginate to distilled water in proportion and mix. After fully mixing, add 0.5% hydrogen peroxide liquid, the liquid accounts for 1%-2% of the total mixture. After fully mixing, let stand for 1-2 hours;

[0058] Step 2: After the standing time is completed, poly (N-isopropylacrylamide), polyethylene glycol and nanocellulose are added in proportion, mixed thoroughly and allowed to stand for 20-30 minutes;

[0059] Step 3, adjusting the pH value of the mixed solution to 4-5 and the temperature value to 22-28°C, and then maintaining the pH value and temperature value for 0.5-1h;

[0060] Step 4: air-dry the mixture to remove excess liquid until the mixture becomes a semi-dry film;

[0061] Step 5: Apply the semi-dry film evenly on the surface of the base. No bubbles should appear during the application process. Apply it in layers until the required Chinese medicine active coating is formed.

[0062] Comparative Example 3

[0063] This embodiment proposes a traditional Chinese medicine active coating based on temperature and pH dual-responsive hydrogel, which includes, by weight: 15 parts of chitosan, 35 parts of hyaluronic acid, 10 parts of sodium alginate, 55 parts of poly (N-isopropylacrylamide), 15 parts of polyacrylic acid, 15 parts of nanocellulose, and 30 parts of baicalein.

[0064] The preparation method of the traditional Chinese medicine active coating comprises the following steps:

[0065] Step 1: Add chitosan, hyaluronic acid and sodium alginate to distilled water in proportion and mix. After fully mixing, add 0.5% hydrogen peroxide liquid, the liquid accounts for 1%-2% of the total mixture. After fully mixing, let stand for 1-2 hours;

[0066] Step 2: After the standing time is completed, poly (N-isopropylacrylamide), polyacrylic acid, and nanocellulose are added in proportion, mixed thoroughly, and allowed to stand for 20-30 minutes;

[0067] Step 3, adjusting the pH value of the mixed solution to 4-5 and the temperature value to 22-28°C, and then maintaining the pH value and temperature value for 0.5-1h;

[0068] Step 4: air-dry the mixture to remove excess liquid until the mixture becomes a semi-dry film;

[0069] Step 5: Apply the semi-dry film evenly on the surface of the base. No bubbles should appear during the application process. Apply it in layers until the required Chinese medicine active coating is formed.

[0070] The present invention monitors the anti-inflammatory index in real time in a simulated wound microenvironment by combining temperature and pH changes, wherein the anti-inflammatory index is expressed by white blood cell counts, and finally the following table is obtained:

[0071] temperature pH Example 1 5.0×10^9 / L 4.0×10^9 / L Example 2 5.5×10^9 / L 5.5×10^9 / L Example 3 9.0×10^9 / L 8.0×10^9 / L Comparative Example 1 12.0×10^9 / L 14.0×10^9 / L Comparative Example 2 15.0×10^9 / L 10.5×10^9 / L Comparative Example 3 4.5×10^9 / L 6.5×10^9 / L

[0072] The following conclusions can be drawn from the above table:

[0073] By comparing Example 1, Example 2 and Example 3 together, it can be found that with the increase of temperature and pH value, the anti-inflammatory effect will decrease; by comparing Example 3 and Comparative Example 1 together, it can be found that under the same temperature and pH value, the lack of nanocellulose will cause a certain offset of the ostomy bag bottom plate, which will cause the patient's wound to be infected again; by comparing Example 3 and Comparative Example 2 together, it can be found that under the same temperature and pH value, the lack of polyacrylic acid will reduce the cross-linking degree of poly (N-isopropylacrylamide) and baicalein, thereby affecting the release rate and release effect of baicalein; by comparing Example 3 and Comparative Example 3 together, it can be found that under the same temperature and pH value, the lack of polyethylene glycol will reduce the cross-linking degree of poly (N-isopropylacrylamide) and baicalein to a certain extent, but the effect is not significant.

[0074] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A traditional Chinese medicine active coating based on temperature and pH dual-responsive hydrogel, characterized in that: The invention comprises, by weight: 10-20 parts of chitosan, 25-50 parts of hyaluronic acid, 5-15 parts of sodium alginate, 30-80 parts of poly (N-isopropylacrylamide), 10-25 parts of polyacrylic acid, 5-10 parts of polyethylene glycol, 10-20 parts of nanocellulose and 20-40 parts of baicalein.

2. The Chinese medicine active coating based on temperature and pH value dual-responsive hydrogel according to claim 1, characterized in that: The invention comprises, by weight: 15 parts of chitosan, 35 parts of hyaluronic acid, 10 parts of sodium alginate, 55 parts of poly (N-isopropylacrylamide), 15 parts of polyacrylic acid, 8 parts of polyethylene glycol, 15 parts of nanocellulose and 30 parts of baicalein.

3. The Chinese medicine active coating based on temperature and pH value dual-responsive hydrogel according to claim 1, characterized in that: The invention also comprises, by weight: 20-40 parts of fluorescent markers.

4. The Chinese medicine active coating based on temperature and pH value dual-responsive hydrogel according to claim 3, characterized in that: The composition comprises, by weight: 15 parts of chitosan, 35 parts of hyaluronic acid, 10 parts of sodium alginate, 55 parts of poly (N-isopropylacrylamide), 15 parts of polyacrylic acid, 8 parts of polyethylene glycol, 15 parts of nanocellulose, 30 parts of baicalein and 30 parts of fluorescent marker.

5. A method for preparing a traditional Chinese medicine active coating based on a temperature and pH value dual-responsive hydrogel, applied to the traditional Chinese medicine active coating based on a temperature and pH value dual-responsive hydrogel according to any one of claims 1 to 4, characterized in that: The following steps are involved: Step 1: Add chitosan, hyaluronic acid and sodium alginate to distilled water in proportion and mix. After fully mixing, add 0.5% hydrogen peroxide liquid, the liquid accounts for 1%-2% of the total mixture. After fully mixing, let stand for 1-2 hours; Step 2: After the standing time is completed, poly (N-isopropylacrylamide), polyacrylic acid, polyethylene glycol and nanocellulose are added in proportion, mixed thoroughly and allowed to stand for 20-30 minutes; Step 3, adjusting the pH value of the mixed solution to 4-5 and the temperature value to 22-28°C, and then maintaining the pH value and temperature value for 0.5-1h; Step 4: air-dry the mixture to remove excess liquid until the mixture becomes a semi-dry film; Step 5: Apply the semi-dry film evenly on the surface of the base. No bubbles should appear during the application process. Apply it in layers until the required Chinese medicine active coating is formed.

6. The method for preparing the active coating of traditional Chinese medicine based on temperature and pH value dual-responsive hydrogel according to claim 5, characterized in that: In the step 1, chitosan, hyaluronic acid and sodium alginate are mixed by stirring, and bubbles are prevented from being mixed into the mixture during the mixing process.

7. The method for preparing the traditional Chinese medicine active coating based on the temperature and pH value dual-responsive hydrogel according to claim 5, characterized in that: In step 4, the wind speed and wind temperature of air drying can be adjusted, the wind speed is less than 1 m / s; the wind temperature is less than 50°C.

8. The method for preparing the traditional Chinese medicine active coating based on temperature and pH value dual-responsive hydrogel according to claim 5, characterized in that: In step five, the number of layers applied in layers is greater than three.