Portable cell culture device for tumor immunotherapy and use method of portable cell culture device

Abstract

The invention provides a portable cell culture device for tumor immunotherapy and a using method thereof.The portable cell culture device comprises a culture shell, a sealing cover is detachably arranged at the top of the culture shell, a plurality of frame bodies used for supporting culture boxes are arranged in the culture shell, the culture boxes are arranged in the frame bodies, a heating body is arranged on the inner wall of the culture shell, and the heating body is arranged on the inner wall of the culture shell. The heating body heats the space around the culture box, a bottom shell is arranged below the culture shell, a rechargeable battery pack and a control mainboard connected with the rechargeable battery pack are arranged in the bottom shell, and the control mainboard is connected with the heating body. When the culture box is placed in the third supporting ring, a heavy object is placed in the center of the third supporting ring, and when the culture box inclines, the culture box can still be kept horizontal all the time under the cooperation of the first supporting ring, the second supporting ring and the third supporting ring, so that when the cell culture device is carried, the culture box is not prone to falling off. No matter the culture shell shakes or inclines, the culture box can be kept stable.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a portable cell culture device for tumor immunotherapy and a use method thereof. Background Art

[0002] Tumor immunotherapy is a treatment method that controls and eliminates tumors by restarting and maintaining the tumor-immune cycle and restoring the body's normal anti-tumor immune response. It includes monoclonal antibody immune checkpoint inhibitors, therapeutic antibodies, cancer vaccines, cell therapy, and small molecule inhibitors. During tumor immunotherapy, cells need to be cultured in order to observe the cell status during tumor immunotherapy.

[0003] However, during the cell culture process, if the cell culture device is carried or transported, when the cell culture device is tilted, the culture dish inside it will also tilt, causing the nutrient solution inside the culture dish to shake and stick to various places inside the culture dish. To this end, we propose a portable cell culture device for tumor immunotherapy and a method of using the same. Summary of the invention

[0004] In view of the disadvantage that if the existing cell culture device tilts during transportation, the culture dish inside it will also tilt, the present invention provides a portable cell culture device for tumor immunotherapy and a method of using the same, which has the advantage of allowing the culture box to always remain horizontally placed without tilting or falling over, thereby solving the problems raised in the above-mentioned background technology.

[0005] The technical solution of the present invention is implemented as follows: a portable cell culture device for tumor immunotherapy is designed, including a culture shell, a sealing cover is detachably provided on the top of the culture shell, a culture box is provided in the culture shell, a plurality of frames for supporting the culture box are provided in the culture shell, the culture box is placed in the frame, a heating element is provided on the inner wall of the culture shell, the heating element heats the space around the culture box, a bottom shell is provided below the culture shell, a rechargeable battery pack and a control mainboard connected to the rechargeable battery pack are provided in the bottom shell, and the control mainboard is connected to the heating element.

[0006] Preferably, a handle is rotatably mounted on the middle of the top of the sealing cover, and the sealing cover is covered on the top of the culture shell and the edges of the two are connected by a connecting buckle.

[0007] Preferably, a temperature sensor is provided in the culture shell, and the temperature sensor is connected to the control main board.

[0008] Preferably, two air circulation channels are symmetrically arranged on the side of the culture shell, filters are arranged in the air circulation channels, and an exhaust fan is installed in one of the air circulation channels, and the exhaust fan is connected to the control mainboard.

[0009] Preferably, a display screen and operation buttons are provided on the side of the bottom shell, and the display screen and the operation buttons are respectively connected to the control mainboard.

[0010] Preferably, each frame includes a first support ring, two first support shafts are symmetrically installed on the side of the first support ring, the first support shaft is rotatably arranged in a fixed frame, the fixed frame is installed on the culture shell, a second support ring is arranged in the first support ring, two third support shafts are symmetrically installed on the side of the second support ring, the third support shaft is perpendicular to the support shaft, and the third support shafts are rotatably connected to the first support ring respectively, a third support ring is arranged in the second support ring, two second support shafts are symmetrically installed on the side of the third support ring, the second support shaft is perpendicular to the third support shaft, and the second support shafts are rotatably connected to the second support ring respectively, wherein each culture box is arranged in the third support ring, and a detachable box cover is provided on the top of the culture box.

[0011] Preferably, the fixing frame comprises a bottom plate located below the culture box, with brackets vertically arranged at both ends of the top of the bottom plate, the tops of the two brackets are rotatably connected to the first support shaft respectively, and the bottom plate is detachably connected to the bottom of the culture shell.

[0012] Preferably, an annular support plate is provided on the inner wall of the third support ring, and an annular limiting plate is provided on the top of the side of the culture box. When the culture box is placed in the third support ring, the annular limiting plate is placed on the annular support plate.

[0013] Preferably, an annular support pad is provided at the bottom edge of the culture box, and a mounting groove is provided in the middle of the bottom surface of the culture box, and a counterweight block is installed in the mounting groove.

[0014] The present invention also provides a method for using a portable cell culture device for tumor immunotherapy, comprising the following steps:

[0015] Step 1: Place the cell culture medium and sampled cells in the culture box, close the box lid, and shake the culture box slightly to allow the cell culture medium and sampled cells to fully contact;

[0016] Step 2: Then number the culture boxes and place them in the frame in the order of the numbers, that is, the culture boxes are placed in the third support circle;

[0017] Step 3: Turn on the heating element to heat the inner space of the culture shell to the culture temperature;

[0018] Step 4: Regular checks are required during the cultivation process.

[0019] Compared with the prior art, when the culture box of the present invention is in use, when it is placed in the third support ring, it is equivalent to placing a heavy object at the center of the third support ring. When the culture box is tilted, the culture box can still remain horizontal with the cooperation of the first support ring, the second support ring and the third support ring. Therefore, when the cell culture device is transported, the culture box can remain stable regardless of whether the culture shell shakes or tilts. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 It is a schematic diagram of the structure of the present invention.

[0022] Figure 2 The internal structure of the present invention is shown in FIG. Figure 1 .

[0023] Figure 3 The internal structure of the present invention is shown in FIG. Figure 2 .

[0024] Figure 4 It is a side view of the present invention.

[0025] Figure 5 It is a structural schematic diagram of the frame of the present invention.

[0026] Figure 6 It is a schematic diagram of the structure of the culture box of the present invention.

[0027] In the figure: 1. bottom shell; 2. air circulation channel; 3. culture shell; 4. handle; 5. sealing cover; 6. connecting buckle; 7. display screen; 8. operation button; 9. first support ring; 10. second support ring; 11. bracket; 12. bottom plate; 13. box cover; 14. rechargeable battery pack; 15. control main board; 16. culture box; 17. first support shaft; 18. second support shaft; 19. annular support plate; 20. third support shaft; 21. annular limit plate; 22. annular support pad; 23. mounting groove; 24. counterweight block; 25. third support ring; 26. heating element. DETAILED DESCRIPTION

[0028] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Reference Figures 1 to 6 The present invention provides a technical solution: a portable cell culture device for tumor immunotherapy, comprising a culture shell 3, a sealing cover 5 is detachably provided on the top of the culture shell 3, a handle 4 is rotatably installed on the middle of the top of the sealing cover 5, and the sealing cover 5 covers the top of the culture shell 3 and the edges of the two are connected by a connecting buckle 6, which is specifically a hasp, so that the cell culture device can be transported and carried after the sealing cover 5 is covered.

[0030] A plurality of fixing frames are arranged in the culture shell 3, and the fixing frames include a bottom plate 12, and brackets 11 are vertically arranged at both ends of the top of the bottom plate 12, and the bottom plate 12 is detachably connected to the bottom of the culture shell 3, that is, the two are fastened by bolts;

[0031] Next, a culture box 16 is provided in the culture housing 3. A plurality of frames for supporting the culture box 16 are also provided in the culture housing 3. The culture box 16 is placed in the frames. Figure 3 and Figure 4 As shown, each frame includes a first support ring 9, and two first support shafts 17 are symmetrically mounted on the side of the first support ring 9. The first support shafts 17 are rotatably arranged in the fixed frame, as shown in FIG. Figure 5 As shown, specifically, the tops of the two brackets 11 are rotatably connected to the first support shaft 17, so that the first support ring 9 can rotate freely around the first support shaft 17;

[0032] A second support ring 10 is provided inside the first support ring 9, and two third support shafts 20 are symmetrically installed on the side of the second support ring 10, the third support shafts 20 are perpendicular to the first support shaft 17, and the third support shafts 20 are rotatably connected to the first support ring 9 respectively, and the second support ring 10 can rotate around the third support shafts 20;

[0033] A third support ring 25 is provided inside the second support ring 10, and two second support shafts 18 are symmetrically installed on the side of the third support ring 25, the second support shafts 18 are perpendicular to the third support shaft 20, and the second support shafts 18 are rotatably connected to the second support ring 10 respectively, and the third support ring 25 can rotate around the second support shafts 18;

[0034] When placing the culture box 16, Figure 3As shown, each culture box 16 is arranged in the third support ring 25, and a box cover 13 is detachably provided on the top of the culture box 16. At this time, the bottom of the culture box 16 is located below the third support ring 25, and the box cover 13 is located above the third support ring 25. When the first support ring 9, the second support ring 10 and the third support ring 25 are placed horizontally, the three rings are coaxially arranged;

[0035] It should be noted that the culture box 16 can always remain stable, and its central axis is always vertically downward, that is, the culture box 16 always remains horizontal and will not tilt. Figure 3 As shown, when the culture box 16 is placed in the third support ring 25, the culture box 16 is located above the bottom plate 12. Because the first support ring 9, the second support ring 10 and the third support ring 25 can all rotate freely, when the culture box 16 is placed in the third support ring 25, it is equivalent to placing a heavy object at the center of the third support ring 25. The culture box 16 always remains horizontal under the drive of gravity. When the culture box 16 is skewed, the culture box 16 can still remain horizontal under the cooperation of the first support ring 9, the second support ring 10 and the third support ring 25. Therefore, when the cell culture device is carried, no matter the culture housing 3 shakes or tilts, the culture box 16 can remain stable.

[0036] Furthermore, because cell culture requires specific temperatures, e.g. Figure 3 As shown, a heating element 26 is provided on the inner wall of the culture shell 3, and the heating element 26 heats the space around the culture box 16. The heat emitted by the heating element 26 does not directly contact the culture box 16, but heats the space around the culture box 16, so that the culture box 16 is heated evenly;

[0037] A bottom shell 1 is provided below the culture shell 3, and a rechargeable battery pack 14 and a control mainboard 15 connected to the rechargeable battery pack 14 are provided in the bottom shell 1, and the control mainboard 15 is connected to the heating element 26. A temperature sensor is provided in the culture shell 3, and the temperature sensor is connected to the control mainboard 15. The temperature sensor is located to detect the temperature in the culture shell 3. Through the feedback value of the temperature sensor, the control mainboard 15 autonomously controls the heating element 26 to generate heat, so that the heating element 26 always maintains constant temperature and heat;

[0038] A display screen 7 and operation buttons 8 are provided on the side of the bottom shell 1. The display screen 7 and the operation buttons 8 are respectively connected to the control main board 15. The operation buttons 8 include temperature adjustment buttons, switch buttons, etc.

[0039] However, in actual operation, if the temperature in the culture shell 3 is too high, it needs to be cooled down, so two air circulation channels 2 are symmetrically arranged on the side of the culture shell 3, and filters are arranged in the air circulation channels 2, and an exhaust fan is installed in one of the air circulation channels 2, and the exhaust fan is connected to the control main board 15. When the exhaust fan is turned on, external air-cooled air can enter the culture shell 3 from the air circulation channel 2, and hot air is discharged to the outside through the exhaust fan. It should be noted that the filter here can filter bacteria and viruses to prevent external pollutants from entering the culture shell 3.

[0040] Further, such as Figure 5 As shown, an annular support plate 19 is provided on the inner wall of the third support ring 25;

[0041] Correspondingly, an annular limiting plate 21 is provided on the top of the side of the culture box 16. When the culture box 16 is placed in the third support ring 25, the annular limiting plate 21 is placed on the annular support plate 19. An annular support pad 22 is provided on the bottom edge of the culture box 16, and a mounting groove 23 is provided in the middle of the bottom surface of the culture box 16. A counterweight block 24 is installed in the mounting groove 23;

[0042] It should be emphasized here that the counterweight 24 can make the culture box 16 more stable. Because the counterweight 24 can make the center of gravity of the culture box 16 heavier, the culture box 16 can be placed more stably in the annular support plate 19, and the gap between the annular support plate 19 and the culture box 16 is matched, so the culture box 16 can be limited by the annular support plate 19. Therefore, no matter how the culture shell 3 is tilted or tilted, the culture box 16 will not fall and will always remain level.

[0043] Furthermore, the present invention also provides a method for using a portable cell culture device for tumor immunotherapy, comprising the following steps:

[0044] Step 1: Place the cell culture medium and sampled cells in the culture box 16, close the box cover 13, and gently shake the culture box 16 to allow the cell culture medium and sampled cells to fully contact;

[0045] Step 2: Then number the culture boxes 16 and place them in the frame in the order of the numbers, that is, the culture boxes 16 are placed in the third support circle 25;

[0046] Step 3: Turn on the heating element 26 to heat the interior space of the culture shell 3 to the culture temperature;

[0047] Step 4: Regular checks are required during the cultivation process.

[0048] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A portable cell culture device for tumor immunotherapy, comprising a culture shell (3), the top of which is detachably provided with a sealing cover (5), characterized in that: A culture box (16) is provided in the culture housing (3); A plurality of frames for supporting the culture box (16) are arranged in the culture shell (3), the culture box (16) is placed in the frames, a heating element (26) is arranged on the inner wall of the culture shell (3), and the heating element (26) heats the space around the culture box (16); A bottom shell (1) is provided below the culture shell (3), and a rechargeable battery pack (14) and a control mainboard (15) connected to the rechargeable battery pack (14) are provided in the bottom shell (1), and the control mainboard (15) is connected to the heating element (26).

2. The portable cell culture device for tumor immunotherapy according to claim 1, characterized in that: A handle (4) is rotatably mounted on the middle of the top of the sealing cover (5), and the sealing cover (5) covers the top of the culture shell (3), and the edges of the two are connected by a connecting buckle (6).

3. The portable cell culture device for tumor immunotherapy according to claim 2, characterized in that: A temperature sensor is provided in the culture shell (3), and the temperature sensor is connected to the control main board (15).

4. The portable cell culture device for tumor immunotherapy according to claim 3, characterized in that: Two air circulation channels (2) are symmetrically arranged on the side of the culture shell (3), filters are arranged in the air circulation channels (2), and an exhaust fan is installed in one of the air circulation channels (2), and the exhaust fan is connected to the control main board (15).

5. The portable cell culture device for tumor immunotherapy according to claim 4, characterized in that: A display screen (7) and operating buttons (8) are provided on the side of the bottom shell (1), and the display screen (7) and operating buttons (8) are respectively connected to the control main board (15).

6. The portable cell culture device for tumor immunotherapy according to claim 5, characterized in that: Each frame body comprises a first support ring (9), two first support shafts (17) are symmetrically mounted on the side of the first support ring (9), the first support shafts (17) are rotatably arranged in a fixed frame, and the fixed frame is mounted on the culture shell (3); A second support ring (10) is arranged inside the first support ring (9), and two third support shafts (20) are symmetrically mounted on the side of the second support ring (10), the third support shafts (20) are perpendicular to the support shaft (17), and the third support shafts (20) are rotatably connected to the first support ring (9) respectively; A third support ring (25) is arranged inside the second support ring (10), and two second support shafts (18) are symmetrically mounted on the side of the third support ring (25), the second support shafts (18) are perpendicular to the third support shaft (20), and the second support shafts (18) are rotatably connected to the second support ring (10) respectively; Each culture box (16) is arranged in the third support circle (25), and a detachable box cover (13) is provided on the top of the culture box (16).

7. The portable cell culture device for tumor immunotherapy according to claim 6, characterized in that: The fixing frame comprises a bottom plate (12) located below the culture box (16), brackets (11) are vertically arranged at both ends of the top of the bottom plate (12), the tops of the two brackets (11) are respectively rotatably connected to the first support shaft (17), and the bottom plate (12) is detachably connected to the bottom of the culture shell (3).

8. The portable cell culture device for tumor immunotherapy according to claim 8, characterized in that: An annular support plate (19) is provided on the inner wall of the third support ring (25); An annular limiting plate (21) is provided on the top of the side of the culture box (16). When the culture box (16) is placed in the third supporting ring (25), the annular limiting plate (21) is placed on the annular supporting plate (19).

9. The portable cell culture device for tumor immunotherapy according to claim 8, characterized in that: An annular support pad (22) is provided at the bottom edge of the culture box (16), a mounting groove (23) is provided in the middle of the bottom surface of the culture box (16), and a counterweight block (24) is installed in the mounting groove (23).

10. A method for using the portable cell culture device for tumor immunotherapy according to claim 9, characterized in that: The steps include: Step 1: Place the cell culture medium and sampled cells in the culture box, close the box lid, and shake the culture box slightly to allow the cell culture medium and sampled cells to fully contact; Step 2: Then number the culture boxes and place them in the frame in the order of the numbers, that is, the culture boxes are placed in the third support circle; Step 3: Turn on the heating element to heat the inner space of the culture shell to the culture temperature; Step 4: Regular checks are required during the cultivation process.

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