Biological fermentation sewage treatment agent and preparation process thereof

Abstract

The invention discloses a biological fermentation sewage treatment agent and a preparation process thereof, relates to the technical field of sewage treatment, and aims at preparing the biological fermentation sewage treatment agent which takes biological fermentation as main treatment and has an adsorption treatment mode in order to treat huge environmental hazards generated by wastewater production. Through cooperation of various strains, the defect of activity failure of the sewage treatment agent caused by environmental change can be effectively avoided, bentonite is further used as an adsorbent, dopamine modification is performed on the bentonite, and the bentonite is matched with aldehyde dextran to generate a Schiff base structure, so that the sewage treatment agent has a good treatment effect. Therefore, the bentonite also has a relatively strong chelating capability on metal ions, and the removal of heavy metal ions in a water body is accelerated; and the glucan is used as a polysaccharide, and can be degraded into glucose to serve as a carbon source of the strain, so that the breeding of the sewage treatment agent in the early stage of the strain is accelerated, and the working efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of sewage treatment, in particular to a biological fermentation sewage treatment agent and a preparation process thereof. Background Art

[0002] At present, with the continuous advancement of industrialization, various types of production wastewater have also increased significantly. Among them, the largest amount of wastewater is generated by industries such as chemical industry and metal smelting and processing. This type of wastewater often contains a large amount of complex organic matter and has a very high COD value. Due to different industries, it also contains different types of heavy metals, which pose a serious threat to the ecological environment. Therefore, it is often necessary to treat the wastewater before it can be discharged; At present, biological fermentation treatment of wastewater is a technology that uses microorganisms to treat and degrade pollutants in wastewater. Microorganisms can effectively utilize organic pollutants in wastewater as their own nutrition and energy and continuously degrade the pollutants. It has a very high cost-effectiveness among many wastewater treatment processes. Summary of the invention

[0003] The purpose of the present invention is to provide a biological fermentation sewage treatment agent and a preparation process thereof to solve the problems raised in the prior art.

[0004] To achieve the above object, the present invention provides the following technical solution: a preparation process of a biological fermentation sewage treatment agent, comprising the following steps: S1. Preparation of bio-fermentation microcapsules; S11. Bacillus subtilis, yeast, and Pseudomonas alternifolia were inoculated into the culture solution that was sterilized at high temperature and cooled, and cultured under aerobic conditions until the fermentation was completed. The culture solution was centrifuged and the supernatant was removed to obtain Bacillus subtilis culture solution, yeast culture solution, and Pseudomonas alternifolia culture solution, respectively; S12. After boiling and sterilizing deionized water and cooling to room temperature, sodium alginate was added thereto, and the mixture was stirred to obtain a sodium alginate solution, which was divided into three portions and set aside; The Bacillus subtilis solution, the yeast solution, and the Pseudomonas alternifolia solution prepared in step S11 are added to the sodium alginate solution respectively, and after being mixed evenly, the sodium alginate mixed solution of the three is sprayed respectively to prepare the bio-fermentation bacterial agent microcapsules; S2. Disperse the dextran in deionized water, stir and completely melt, add sulfuric acid dropwise thereto, adjust the pH of the mixed solution to 1-1.5, add sodium periodate thereto, stir and react for 4-8 hours in a dark environment, dialyze to remove the remaining sodium periodate in the mixed solution, dry the remaining product, wash with deionized water at a temperature of 0-4°C, and dry to constant weight to obtain aldehyde-modified dextran; S3. Disperse dopamine hydrochloride in deionized water, add potassium hydroxide dropwise thereto to adjust the pH value to 8-9, add nano-bentonite, heat to 75-85°C, perform ultrasonic dispersion reaction for 4-12h, centrifuge and precipitate, and vacuum dry to constant weight to obtain dopamine bentonite; S4. Disperse the aldehyde-modified glucan into deionized water, stir and mix evenly, add the bio-fermentation bacteria microcapsules therein, continue stirring and mixing for 10-20 minutes, add the dopamine-modified bentonite, mix evenly, soak for 2-12 hours, vacuum dry the mixture to a moisture content of 1-3.5%, crush the resulting product, and pass it through a 40-mesh sieve to obtain a bio-fermentation wastewater treatment agent.

[0005] Furthermore, in step S1, the culture solution consists of 10-15 parts of beef peptone, 10-15 parts of glucose, 0.2-0.5 parts of potassium dihydrogen phosphate, and 65-80 parts of deionized water, by weight.

[0006] Furthermore, in step S1, the concentration of Bacillus subtilis in the Bacillus subtilis liquid is 6-10×1012 cells / mL; the concentration of yeast in the yeast liquid is 3-6×1012 cells / mL; and the concentration of Pseudomonas alternata in the Pseudomonas alternata liquid is 3-6×1012 cells / mL.

[0007] Furthermore, in step S12, the sodium alginate concentration in the sodium alginate solution is 4-6wt%.

[0008] Furthermore, in step S12, during spraying, a container containing a saturated calcium chloride solution is used as a receiving container, and the distance between the spray head and the liquid surface of the saturated calcium chloride solution is 10-15 cm. After the spraying is completed, the saturated calcium chloride solution is filtered, the bacterial liquid microspheres therein are collected, and after washing with a saturated sodium chloride solution, vacuum drying to constant weight, the three products are mixed in equal proportions to prepare bio-fermentation bacterial agent microcapsules.

[0009] Furthermore, in step S2, the mass ratio of dextran to sodium periodate is 10:(2-5).

[0010] Furthermore, in step S3, the mass ratio of dopamine hydrochloride to nano-bentonite is (0.5-1):10.

[0011] Furthermore, in step S4, the mass ratio of the aldehyde-modified glucan, the bio-fermentation bacterial agent microcapsules, and the dopamine-modified bentonite is (1-3): (0.2-1):10.

[0012] Furthermore, a biological fermentation sewage treatment agent is prepared by the above method.

[0013] Compared with the prior art, the present invention has the following beneficial effects: In order to treat the huge environmental hazards caused by industrial wastewater, the present invention prepares a biological fermentation sewage treatment agent that uses biological fermentation as the main treatment method and also has an adsorption treatment method; the present invention uses Bacillus subtilis, yeast, and Pseudomonas alternifolia as the main bacterial species of the sewage treatment agent. These three types of bacterial species can produce and secrete a variety of enzymes that degrade macromolecular organic matter in their life activities, which can effectively accelerate the reduction of COD in sewage, and through the coordination of multiple bacterial species, the activity failure defect of the sewage treatment agent caused by environmental changes can be effectively avoided, so that the sewage treatment agent prepared by the present invention can cope with most sewage environments; On this basis, the present invention further uses microcapsule embedding technology to embed the bacterial solution, thereby avoiding the inactivation problem caused by direct contact of the bacterial strain with sewage and environmental changes during storage, thereby reducing the loss of bacterial strains; In addition, the present invention also uses bentonite as an adsorbent. The particles of bentonite itself are relatively small, and colloids can be formed in water bodies to adsorb and fix large particle pollutants in sewage, thereby purifying the water body. On this basis, the present invention also performs dopamine modification on the bentonite, thereby introducing amino groups into the bentonite and improving the hydrophilicity of the bentonite, thereby enhancing its dispersion stability in the water body. In addition, the present invention further uses dextran as a raw material, and generates aldehyde groups therein after oxidation treatment with sodium periodate. The aldehyde-modified dextran is mixed with the dopamine-modified bentonite, and a Schiff base structure can be generated between the amino groups and the aldehyde groups, thereby making the bentonite have a strong chelating ability for metal ions, thereby accelerating the removal of heavy metal ions in the water body. Moreover, dextran itself, as a polysaccharide, can be degraded into glucose as a carbon source for bacteria, thereby accelerating the reproduction of early bacteria in the sewage treatment agent and improving work efficiency. DETAILED DESCRIPTION

[0014] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present invention.

[0015] Example 1. A process for preparing a biological fermentation sewage treatment agent, comprising the following steps: S1. Preparation of bio-fermentation microcapsules; S11. Bacillus subtilis, yeast, and Pseudomonas alternifolia were inoculated into the culture medium that had been sterilized at high temperature and cooled. Under aerobic conditions, the culture temperature was maintained at 36±1.5℃. After the culture and fermentation, the culture medium was centrifuged and the supernatant was removed to obtain the concentrations of 7.42×10 12 / mL of Bacillus subtilis liquid, with a concentration of 3.54×10 12 / mL yeast solution, with a concentration of 3.67×10 12 Pseudomonas alternata bacterial solution of 100 / mL; Wherein, the culture solution is composed of 12 parts of beef peptone, 14 parts of glucose, 0.4 parts of potassium dihydrogen phosphate, and 70 parts of deionized water in parts by weight; S12. After boiling deionized water for 15 min, standing and cooling to 25 ° C, sodium alginate was added thereto, and after stirring and mixing, a sodium alginate solution with a concentration of 4 wt% was obtained, which was divided into 3 parts and set aside; The Bacillus subtilis solution, yeast solution, and Pseudomonas alternifolia solution prepared in step S11 are added to the sodium alginate solution respectively, and after being evenly mixed, the sodium alginate mixed solution of the three is sprayed respectively. During the spraying, a container containing a saturated calcium chloride solution is used as a receiving container, and the distance between the spray head and the liquid surface of the saturated calcium chloride solution is 10 cm. After the spraying is completed, the saturated calcium chloride solution is filtered, and the bacterial liquid microspheres therein are collected, and after being washed with a saturated sodium chloride solution, vacuum dried to constant weight, and the three dried products are mixed in equal proportions to obtain biological fermentation bacterial agent microcapsules; The volume ratio of the sodium alginate solution to the Bacillus subtilis solution, the yeast solution, and the Pseudomonas alternifolia solution is 1:1. S2. Disperse 10 parts of dextran in deionized water by weight, heat to 75-80°C, stir to completely melt, add sulfuric acid dropwise, adjust the pH of the mixed solution to 1, add 2 parts of sodium periodate, stir to react for 8 hours in a dark environment, dialyze to remove the remaining sodium periodate in the mixed solution, dry the remaining product, wash it 3 times with deionized water at a temperature of 0-4°C, and dry it to constant weight to obtain aldehyde dextran; S3. Disperse 0.5 parts of dopamine hydrochloride in deionized water by weight, stir until dissolved, add potassium hydroxide dropwise to adjust the pH value to 8.5, add 10 parts of nano-bentonite, heat to 80 ° C, disperse by ultrasonic vibration for 8 hours, centrifuge and precipitate, and vacuum dry to constant weight to obtain dopamine bentonite; S4. According to the weight percentage, 1 part of formaldehyded dextran was dispersed in deionized water, the temperature was raised to 45°C, and the mixture was stirred and mixed evenly. The mixture was then cooled to room temperature. 0.2 parts of bio-fermentation bacteria microcapsules were added thereto. The mixture was stirred and mixed for 10 minutes. Then, 10 parts of dopamine-modified bentonite were added and mixed evenly. The mixture was immersed for 8 hours, and the mixture was vacuum dried to a moisture content of 1.5%. The obtained product was crushed and passed through a 40-mesh sieve to obtain a bio-fermentation wastewater treatment agent.

[0016] Example 2. A process for preparing a biological fermentation sewage treatment agent, comprising the following steps: Compared with Example 1, this embodiment increases the concentration of the sodium alginate solution in step S12; S1. Preparation of bio-fermentation microcapsules; S11. Bacillus subtilis, yeast, and Pseudomonas alternifolia were inoculated into the culture medium that had been sterilized at high temperature and cooled. Under aerobic conditions, the culture temperature was maintained at 36±1.5℃. After the culture and fermentation, the culture medium was centrifuged and the supernatant was removed to obtain the concentrations of 7.42×10 12 / mL of Bacillus subtilis liquid, with a concentration of 3.54×10 12 / mL yeast solution, with a concentration of 3.67×10 12 Pseudomonas alternata bacterial solution of 100 / mL; Wherein, the culture solution is composed of 12 parts of beef peptone, 14 parts of glucose, 0.4 parts of potassium dihydrogen phosphate, and 70 parts of deionized water in parts by weight; S12. After boiling deionized water for 15 min, standing and cooling to 25 ° C, sodium alginate was added thereto, and after stirring and mixing, a sodium alginate solution with a concentration of 6 wt% was obtained, which was divided into 3 parts and set aside; The Bacillus subtilis solution, yeast solution, and Pseudomonas alternifolia solution prepared in step S11 are added to the sodium alginate solution respectively, and after being evenly mixed, the sodium alginate mixed solution of the three is sprayed respectively. During the spraying, a container containing a saturated calcium chloride solution is used as a receiving container, and the distance between the spray head and the liquid surface of the saturated calcium chloride solution is 10 cm. After the spraying is completed, the saturated calcium chloride solution is filtered, and the bacterial liquid microspheres therein are collected, and after being washed with a saturated sodium chloride solution, vacuum dried to constant weight, and the three dried products are mixed in equal proportions to obtain biological fermentation bacterial agent microcapsules; The volume ratio of the sodium alginate solution to the Bacillus subtilis solution, the yeast solution, and the Pseudomonas alternifolia solution is 1:1. S2. Disperse 10 parts of dextran in deionized water by weight, heat to 75-80°C, stir to completely melt, add sulfuric acid dropwise, adjust the pH of the mixed solution to 1, add 2 parts of sodium periodate, stir to react for 8 hours in a dark environment, dialyze to remove the remaining sodium periodate in the mixed solution, dry the remaining product, wash it 3 times with deionized water at a temperature of 0-4°C, and dry it to constant weight to obtain aldehyde dextran; S3. Disperse 0.5 parts of dopamine hydrochloride in deionized water by weight, stir until dissolved, add potassium hydroxide dropwise to adjust the pH value to 8.5, add 10 parts of nano-bentonite, heat to 80 ° C, disperse by ultrasonic vibration for 8 hours, centrifuge and precipitate, and vacuum dry to constant weight to obtain dopamine bentonite; S4. According to the weight percentage, 1 part of formaldehyded dextran was dispersed in deionized water, the temperature was raised to 45°C, and the mixture was stirred and mixed evenly. The mixture was then cooled to room temperature. 0.2 parts of bio-fermentation bacteria microcapsules were added thereto. The mixture was stirred and mixed for 10 minutes. Then, 10 parts of dopamine-modified bentonite were added and mixed evenly. The mixture was immersed for 8 hours, and the mixture was vacuum dried to a moisture content of 1.5%. The obtained product was crushed and passed through a 40-mesh sieve to obtain a bio-fermentation wastewater treatment agent.

[0017] Example 3. A process for preparing a biological fermentation sewage treatment agent, comprising the following steps: Compared with Example 2, this example increases the concentration of sodium periodate in step S2; S1. Preparation of bio-fermentation microcapsules; S11. Bacillus subtilis, yeast, and Pseudomonas alternifolia were inoculated into the culture medium that had been sterilized at high temperature and cooled. Under aerobic conditions, the culture temperature was maintained at 36±1.5℃. After the culture and fermentation, the culture medium was centrifuged and the supernatant was removed to obtain the concentrations of 7.42×10 12 / mL of Bacillus subtilis liquid, with a concentration of 3.54×10 12 / mL yeast solution, with a concentration of 3.67×10 12 Pseudomonas alternata bacterial solution of 100 / mL; Wherein, the culture solution is composed of 12 parts of beef peptone, 14 parts of glucose, 0.4 parts of potassium dihydrogen phosphate, and 70 parts of deionized water in parts by weight; S12. After boiling deionized water for 15 min, standing and cooling to 25 ° C, sodium alginate was added thereto, and after stirring and mixing, a sodium alginate solution with a concentration of 6 wt% was obtained, which was divided into 3 parts and set aside; The Bacillus subtilis solution, yeast solution, and Pseudomonas alternifolia solution prepared in step S11 are added to the sodium alginate solution respectively, and after being evenly mixed, the sodium alginate mixed solution of the three is sprayed respectively. During the spraying, a container containing a saturated calcium chloride solution is used as a receiving container, and the distance between the spray head and the liquid surface of the saturated calcium chloride solution is 10 cm. After the spraying is completed, the saturated calcium chloride solution is filtered, and the bacterial liquid microspheres therein are collected, and after being washed with a saturated sodium chloride solution, vacuum dried to constant weight, and the three dried products are mixed in equal proportions to obtain biological fermentation bacterial agent microcapsules; The volume ratio of the sodium alginate solution to the Bacillus subtilis solution, the yeast solution, and the Pseudomonas alternifolia solution is 1:1. S2. Disperse 10 parts of dextran in deionized water by weight, heat to 75-80°C, stir to completely melt, add sulfuric acid dropwise, adjust the pH of the mixed solution to 1, add 5 parts of sodium periodate, stir to react for 8 hours in a dark environment, dialyze to remove the remaining sodium periodate in the mixed solution, dry the remaining product, wash it 3 times with deionized water at a temperature of 0-4°C, and dry it to constant weight to obtain aldehyde dextran; S3. Disperse 0.5 parts of dopamine hydrochloride in deionized water by weight, stir until dissolved, add potassium hydroxide dropwise to adjust the pH value to 8.5, add 10 parts of nano-bentonite, heat to 80 ° C, disperse by ultrasonic vibration for 8 hours, centrifuge and precipitate, and vacuum dry to constant weight to obtain dopamine bentonite; S4. According to the weight percentage, 1 part of formaldehyded dextran was dispersed in deionized water, the temperature was raised to 45°C, and the mixture was stirred and mixed evenly. The mixture was then cooled to room temperature. 0.2 parts of bio-fermentation bacteria microcapsules were added thereto. The mixture was stirred and mixed for 10 minutes. Then, 10 parts of dopamine-modified bentonite were added and mixed evenly. The mixture was immersed for 8 hours, and the mixture was vacuum dried to a moisture content of 1.5%. The obtained product was crushed and passed through a 40-mesh sieve to obtain a bio-fermentation wastewater treatment agent.

[0018] Example 4. A process for preparing a biological fermentation sewage treatment agent, comprising the following steps: Compared with Example 3, this example increases the concentration of dopamine hydrochloride in step S3; S1. Preparation of bio-fermentation microcapsules; S11. Bacillus subtilis, yeast, and Pseudomonas alternifolia were inoculated into the culture medium that had been sterilized at high temperature and cooled. Under aerobic conditions, the culture temperature was maintained at 36±1.5℃. After the culture and fermentation, the culture medium was centrifuged and the supernatant was removed to obtain the concentrations of 7.42×10 12 / mL of Bacillus subtilis liquid, with a concentration of 3.54×10 12 / mL yeast solution, with a concentration of 3.67×10 12 Pseudomonas alternata bacterial solution of 100 / mL; Wherein, the culture solution is composed of 12 parts of beef peptone, 14 parts of glucose, 0.4 parts of potassium dihydrogen phosphate, and 70 parts of deionized water in parts by weight; S12. After boiling deionized water for 15 min, standing and cooling to 25 ° C, sodium alginate was added thereto, and after stirring and mixing, a sodium alginate solution with a concentration of 6 wt% was obtained, which was divided into 3 parts and set aside; The Bacillus subtilis solution, yeast solution, and Pseudomonas alternifolia solution prepared in step S11 are added to the sodium alginate solution respectively, and after being evenly mixed, the sodium alginate mixed solution of the three is sprayed respectively. During the spraying, a container containing a saturated calcium chloride solution is used as a receiving container, and the distance between the spray head and the liquid surface of the saturated calcium chloride solution is 10 cm. After the spraying is completed, the saturated calcium chloride solution is filtered, and the bacterial liquid microspheres therein are collected, and after being washed with a saturated sodium chloride solution, vacuum dried to constant weight, and the three dried products are mixed in equal proportions to obtain biological fermentation bacterial agent microcapsules; The volume ratio of the sodium alginate solution to the Bacillus subtilis solution, the yeast solution, and the Pseudomonas alternifolia solution is 1:1. S2. Disperse 10 parts of dextran in deionized water by weight, heat to 75-80°C, stir to completely melt, add sulfuric acid dropwise, adjust the pH of the mixed solution to 1, add 5 parts of sodium periodate, stir to react for 8 hours in a dark environment, dialyze to remove the remaining sodium periodate in the mixed solution, dry the remaining product, wash it 3 times with deionized water at a temperature of 0-4°C, and dry it to constant weight to obtain aldehyde dextran; S3. Disperse 1 part of dopamine hydrochloride in deionized water by weight, stir until dissolved, add potassium hydroxide dropwise to adjust the pH value to 8.5, add 10 parts of nano-bentonite, heat to 80 ° C, disperse by ultrasonic vibration for 8 hours, centrifuge and precipitate, and vacuum dry to constant weight to obtain dopamine bentonite; S4. According to the weight percentage, 1 part of formaldehyded dextran was dispersed in deionized water, the temperature was raised to 45°C, and the mixture was stirred and mixed evenly. The mixture was then cooled to room temperature. 0.2 parts of bio-fermentation bacteria microcapsules were added thereto. The mixture was stirred and mixed for 10 minutes. Then, 10 parts of dopamine-modified bentonite were added and mixed evenly. The mixture was immersed for 8 hours, and the mixture was vacuum dried to a moisture content of 1.5%. The obtained product was crushed and passed through a 40-mesh sieve to obtain a bio-fermentation wastewater treatment agent.

[0019] Example 5. A process for preparing a biological fermentation sewage treatment agent, comprising the following steps: Compared with Example 4, this example increases the concentration of aldehyde-deoxyglucan in step S4; S4. Disperse 3 parts of formaldehyded dextran in deionized water by weight, heat to 45°C, stir and mix evenly, cool to room temperature, add 0.2 parts of bio-fermentation bacteria microcapsules, continue stirring and mixing for 10 minutes, add 10 parts of dopamine bentonite, mix evenly, soak for 8 hours, vacuum dry the mixture to a moisture content of 1.5%, crush the resulting product, and pass it through a 40-mesh sieve to obtain a bio-fermentation wastewater treatment agent.

[0020] Example 6. A process for preparing a biological fermentation sewage treatment agent, comprising the following steps: Compared with Example 5, this embodiment increases the concentration of the bio-fermentation agent microcapsules in step S4; S4. Disperse 3 parts of formaldehyded dextran in deionized water by weight, heat to 45°C, stir and mix evenly, cool to room temperature, add 1 part of bio-fermentation bacteria microcapsules, continue stirring and mixing for 10 minutes, add 10 parts of dopamine-modified bentonite, mix evenly, soak for 8 hours, vacuum dry the mixture to a moisture content of 1.5%, crush the resulting product, and pass it through a 40-mesh sieve to obtain a bio-fermentation wastewater treatment agent.

[0021] Comparative Example 1. A process for preparing a biological fermentation sewage treatment agent, comprising the following steps: Compared with Example 1, this comparative example only prepared bacterial liquid, but did not prepare microcapsules; S1. Preparing a biological fermentation broth; S11. Bacillus subtilis, yeast, and Pseudomonas alternifolia were inoculated into the culture medium that had been sterilized at high temperature and cooled. Under aerobic conditions, the culture temperature was maintained at 36±1.5℃. After the culture and fermentation, the culture medium was centrifuged and the supernatant was removed to obtain the concentrations of 7.42×10 12 / mL of Bacillus subtilis liquid, with a concentration of 3.54×10 12 / mL yeast solution, with a concentration of 3.67×10 12 Pseudomonas alternata bacterial solution of 100 / mL; Wherein, the culture solution is composed of 12 parts of beef peptone, 14 parts of glucose, 0.4 parts of potassium dihydrogen phosphate, and 70 parts of deionized water in parts by weight; The volume ratio of the sodium alginate solution to the Bacillus subtilis solution, the yeast solution, and the Pseudomonas alternifolia solution is 1:1. S2. Disperse 10 parts of dextran in deionized water by weight, heat to 75-80°C, stir to completely melt, add sulfuric acid dropwise, adjust the pH of the mixed solution to 1, add 2 parts of sodium periodate, stir to react for 8 hours in a dark environment, dialyze to remove the remaining sodium periodate in the mixed solution, dry the remaining product, wash it 3 times with deionized water at a temperature of 0-4°C, and dry it to constant weight to obtain aldehyde dextran; S3. Disperse 0.5 parts of dopamine hydrochloride in deionized water by weight, stir until dissolved, add potassium hydroxide dropwise to adjust the pH value to 8.5, add 10 parts of nano-bentonite, heat to 80 ° C, disperse by ultrasonic vibration for 8 hours, centrifuge and precipitate, and vacuum dry to constant weight to obtain dopamine bentonite; S4. According to the weight percentage, 1 part of formaldehyded dextran was dispersed in deionized water, the temperature was raised to 45°C, the mixture was stirred and mixed evenly, and then the mixture was cooled to room temperature. 0.2 parts of biological fermentation bacteria liquid was added thereto, the mixture was stirred and mixed for 10 minutes, and then 10 parts of dopamine-modified bentonite were added and mixed evenly. After immersion for 8 hours, the mixture was vacuum dried to a moisture content of 1.5%, and the obtained product was crushed and passed through a 40-mesh sieve to obtain a biological fermentation wastewater treatment agent.

[0022] Comparative Example 2. A process for preparing a biological fermentation sewage treatment agent, comprising the following steps: Compared with Example 1, the bentonite was not subjected to dopamination treatment in this comparative example; S1. Preparation of bio-fermentation microcapsules; S11. Bacillus subtilis, yeast, and Pseudomonas alternifolia were inoculated into the culture medium that had been sterilized at high temperature and cooled. Under aerobic conditions, the culture temperature was maintained at 36±1.5℃. After the culture and fermentation, the culture medium was centrifuged and the supernatant was removed to obtain the concentrations of 7.42×10 12 / mL of Bacillus subtilis liquid, with a concentration of 3.54×10 12 / mL yeast solution, with a concentration of 3.67×10 12 Pseudomonas alternata bacterial solution of 100 / mL; Wherein, the culture solution is composed of 12 parts of beef peptone, 14 parts of glucose, 0.4 parts of potassium dihydrogen phosphate, and 70 parts of deionized water in parts by weight; S12. After boiling deionized water for 15 min, standing and cooling to 25 ° C, sodium alginate was added thereto, and after stirring and mixing, a sodium alginate solution with a concentration of 4 wt% was obtained, which was divided into 3 parts and set aside; The Bacillus subtilis solution, yeast solution, and Pseudomonas alternifolia solution prepared in step S11 are added to the sodium alginate solution respectively, and after being evenly mixed, the sodium alginate mixed solution of the three is sprayed respectively. During the spraying, a container containing a saturated calcium chloride solution is used as a receiving container, and the distance between the spray head and the liquid surface of the saturated calcium chloride solution is 10 cm. After the spraying is completed, the saturated calcium chloride solution is filtered, and the bacterial liquid microspheres therein are collected, and after being washed with a saturated sodium chloride solution, vacuum dried to constant weight, and the three dried products are mixed in equal proportions to obtain biological fermentation bacterial agent microcapsules; The volume ratio of the sodium alginate solution to the Bacillus subtilis solution, the yeast solution, and the Pseudomonas alternifolia solution is 1:1. S2. Disperse 10 parts of dextran in deionized water by weight, heat to 75-80°C, stir to completely melt, add sulfuric acid dropwise, adjust the pH of the mixed solution to 1, add 2 parts of sodium periodate, stir to react for 8 hours in a dark environment, dialyze to remove the remaining sodium periodate in the mixed solution, dry the remaining product, wash it 3 times with deionized water at a temperature of 0-4°C, and dry it to constant weight to obtain aldehyde dextran; S3. According to the weight percentage, 1 part of formaldehyded dextran was dispersed in deionized water, the temperature was raised to 45°C, and the mixture was stirred and mixed evenly. The mixture was then cooled to room temperature. 0.2 parts of bio-fermentation bacteria microcapsules were added thereto. The mixture was stirred and mixed for 10 minutes. Then, 10 parts of nano-bentonite were added and mixed evenly. The mixture was immersed for 8 hours, and the mixture was vacuum dried to a moisture content of 1.5%. The obtained product was crushed and passed through a 40-mesh sieve to obtain a bio-fermentation wastewater treatment agent.

[0023] Detection: Take the wastewater produced by a factory, add the biological fermentation wastewater treatment agent prepared in Example 1-6 and Comparative Example 1-2 at a ratio of 20 g / L, treat it for 72 hours, and then detect the water condition. The test results are shown in the following table;

[0024] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A preparation process of a biological fermentation sewage treatment agent, characterized in that: The following steps are involved: S1. Preparation of bio-fermentation microcapsules; S11. Bacillus subtilis, yeast, and Pseudomonas alternifolia were inoculated into the culture solution that was sterilized at high temperature and cooled, and cultured under aerobic conditions until the fermentation was completed. The culture solution was centrifuged and the supernatant was removed to obtain Bacillus subtilis culture solution, yeast culture solution, and Pseudomonas alternifolia culture solution, respectively; S12. After boiling and sterilizing deionized water and cooling to room temperature, sodium alginate was added thereto, and the mixture was stirred and mixed to obtain a sodium alginate solution, which was divided into three portions and set aside; The Bacillus subtilis solution, the yeast solution, and the Pseudomonas alternifolia solution prepared in step S11 are added to the sodium alginate solution respectively, and after being mixed evenly, the sodium alginate mixed solution of the three is sprayed respectively to prepare the bio-fermentation bacterial agent microcapsules; S2. Disperse the dextran in deionized water, stir and completely melt, add sulfuric acid dropwise thereto, adjust the pH of the mixed solution to 1-1.5, add sodium periodate thereto, stir and react for 4-8 hours in a dark environment, dialyze to remove the remaining sodium periodate in the mixed solution, dry the remaining product, wash with deionized water at a temperature of 0-4°C, and dry to constant weight to obtain aldehyde-modified dextran; S3. Disperse dopamine hydrochloride in deionized water, add potassium hydroxide dropwise thereto to adjust the pH value to 8-9, add nano-bentonite, heat to 75-85°C, perform ultrasonic dispersion reaction for 4-12h, centrifuge and precipitate, and vacuum dry to constant weight to obtain dopamine bentonite; S4. Disperse the aldehyde-modified glucan into deionized water, stir and mix evenly, add the bio-fermentation bacteria microcapsules therein, continue stirring and mixing for 10-20 minutes, add the dopamine-modified bentonite, mix evenly, soak for 2-12 hours, vacuum dry the mixture to a moisture content of 1-3.5%, crush the resulting product, and pass it through a 40-mesh sieve to obtain a bio-fermentation wastewater treatment agent.

2. The preparation process of a biological fermentation sewage treatment agent according to claim 1, characterized in that: In step S1, the culture solution consists of 10-15 parts of beef peptone, 10-15 parts of glucose, 0.2-0.5 parts of potassium dihydrogen phosphate, and 65-80 parts of deionized water, by weight.

3. The preparation process of a biological fermentation sewage treatment agent according to claim 1, characterized in that: In step S1, the concentration of Bacillus subtilis in the Bacillus subtilis liquid is 6-10×10 12 The yeast concentration in the yeast solution is 3-6×10 12 The concentration of Pseudomonas alternata in the Pseudomonas alternata bacterial solution is 3-6×10 12 Pieces / mL.

4. The preparation process of a biological fermentation sewage treatment agent according to claim 1, characterized in that: In step S12, the sodium alginate concentration in the sodium alginate solution is 4-6wt%.

5. The preparation process of a biological fermentation sewage treatment agent according to claim 1, characterized in that: In step S12, during spraying, a container containing a saturated calcium chloride solution is used as a receiving container, and the distance between the spray head and the liquid surface of the saturated calcium chloride solution is 10-15 cm. After the spraying is completed, the saturated calcium chloride solution is filtered, the bacterial liquid microspheres therein are collected, and after washing with a saturated sodium chloride solution, vacuum drying to constant weight, the three products are mixed in equal proportions to prepare bio-fermentation bacterial agent microcapsules.

6. The preparation process of a biological fermentation sewage treatment agent according to claim 1, characterized in that: In step S2, the mass ratio of dextran to sodium periodate is 10:(2-5).

7. The process for preparing a biological fermentation sewage treatment agent according to claim 1, characterized in that: In step S3, the mass ratio of dopamine hydrochloride to nano-bentonite is (0.5-1):

10.

8. The process for preparing a biological fermentation sewage treatment agent according to claim 1, characterized in that: In step S4, the mass ratio of the aldehyde-modified glucan, the bio-fermentation bacterial agent microcapsules, and the dopamine-modified bentonite is (1-3): (0.2-1):

10.

9. A biological fermentation wastewater treatment agent prepared by the preparation process according to any one of claims 1 to 8.

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