Cold resistance detection device and method for evaluating the cold resistance of Pennisetum alopecuroides based on spectral analysis

Abstract

The present invention relates to the technical field of cold resistance evaluation, and particularly to a cold resistance detection device and a method for evaluating the cold resistance of Pennisetum alopecuroides based on spectral analysis. The device includes a containing tube, a reflux condenser tube, an extension tube, a liquid supplement tube, and a control core. When the air flow lifts the weight plugging member, the core body pushes open the valve, thereby realizing the addition of the solvent. The method includes: performing low-temperature treatment on the Pennisetum alopecuroides detection sample, and detecting the low-temperature exudation concentration of potassium ions by atomic absorption spectrometry in a solvent with a preset dosage; after the heat treatment is completed, replenishing the solvent amount in the containing tube to the preset dosage, and detecting the high-temperature exudation concentration of potassium ions by atomic absorption spectrometry; determining the low-temperature exudation rate of potassium ions according to the low-temperature exudation concentration and the high-temperature exudation concentration, and evaluating the cold resistance of the Pennisetum alopecuroides detection sample according to the low-temperature exudation rate. It simultaneously realizes small dosage, high efficiency, and low risk in the detection process, ensuring the efficient and smooth progress of the detection work.

Description

Technical Field

[0001] The present invention relates to the technical field of cold resistance evaluation, and more particularly, to a cold resistance detection device and a method for evaluating the cold resistance of Pennisetum alopecuroides based on spectral analysis. Background Art

[0002] In the research work on cold resistance, it is often necessary to detect the potassium ion leakage rate under different low-temperature environments. In the prior art, when detecting the potassium ion leakage rate, in order to improve the detection efficiency and reduce the detection cost, usually a smaller number of detection samples are used in combination with a smaller amount of solvent for detection. When adopting this method, when detecting the complete release concentration of potassium ions, it is usually necessary to boil and maintain for a period of time, and in this process, the solvent often accidentally dries up, resulting in the failure of the experiment. In order to avoid the accidental drying up of the solvent, it is usually necessary for the staff to keep watch during the heat treatment time, which causes a large physical burden.

[0003] Although using a larger number of detection samples in combination with a larger amount of solvent for detection can reduce the probability of accidental drying up of the solvent, the energy consumed during its heat treatment process will increase significantly. At the same time, due to the large amount of solvent used, the risk of scalding increases, and the volume of the equipment will also increase, reducing the operational convenience.

[0004] In view of this, the present application is specifically proposed. Summary of the Invention

[0005] The first object of the present invention is to provide a cold resistance detection device, which can effectively reduce the probability of accidental drying up of the solvent while realizing the detection with a smaller number of detection samples in combination with a smaller amount of solvent, ensuring the efficient and smooth progress of the detection work.

[0006] The second object of the present invention is to provide a method for evaluating the cold resistance of Pennisetum alopecuroides based on spectral analysis, which simultaneously realizes small consumption, high efficiency and low risk in the detection process, ensuring the efficient and smooth progress of the detection work.

[0007] The embodiments of the present invention are implemented as follows:

[0008] A cold resistance detection device includes: a containing tube, a reflux condenser tube, an extension tube, a liquid supplement tube and a control core.

[0009] The containing tube is used to contain the detection sample and the solvent. The containing tube is communicated with the reflux condenser tube, and the extension tube is connected to the end of the reflux condenser tube far away from the containing tube and is communicated with the reflux condenser tube. A drain port is provided on the side wall of the extension tube. One end of the liquid supplement tube is sealed by a sealing block, and the end of the extension tube far away from the reflux condenser tube is connected to the sealing block. The sealing block is provided with a communication hole for communicating the liquid supplement tube and the extension tube, and a valve is fitted at the end of the communication hole far away from the extension tube. When the liquid supplement tube contains the solvent, the valve is closed.

[0010] The control core includes a core body and a weight plugging member. The core body penetrates through the weight plugging member and is fixedly connected to the weight plugging member. The diameter of the core body is smaller than the inner diameter of the reflux condenser tube. One end of the core body is fitted inside the reflux condenser tube, and the other end of the core body is fitted into the communication hole. The weight plugging member is located inside the extension tube. In the natural state, the weight plugging member abuts against the end of the reflux condenser tube far from the receiving tube, thereby plugging the reflux condenser tube.

[0011] When the air flow lifts the weight plugging member, the core body pushes open the valve.

[0012] Furthermore, the control core further includes: a drainage needle.

[0013] The core body has an axial through-hole extending along its axis and penetrating it. The drainage needle is accommodated inside the core body and is arranged along the axis of the core body. The diameter of the drainage needle is smaller than the inner diameter of the core body, and the drainage needle is fixedly connected to the core body.

[0014] The drainage needle extends beyond the end of the core body far from the receiving tube. When the air flow lifts the weight plugging member, the drainage needle pushes open the valve.

[0015] Furthermore, a radial rod is fixedly connected inside the receiving tube. The radial rod is fixedly connected with a fitting column. The fitting column is coaxially arranged with the core body, and the diameter of the fitting column is adapted to the inner diameter of the core body. The core body extends into the receiving tube, and the fitting column is fitted inside the core body.

[0016] When the air flow lifts the weight plugging member, the core body moves towards the liquid replenishing tube and separates from the fitting column.

[0017] Furthermore, a separator is provided inside the receiving tube. The separator includes a first mesh plate, a connecting column, and a second mesh plate.

[0018] Both the first mesh plate and the second mesh plate are arranged perpendicular to the axis of the receiving tube and are spaced apart. The first mesh plate is located on the side of the second mesh plate close to the bottom of the receiving tube. The connecting column fixedly connects the first mesh plate and the second mesh plate.

[0019] The separator is detachably installed inside the receiving tube, and the separator is located on the side of the fitting column far from the bottom of the receiving tube.

[0020] The connecting column is coaxially arranged with the core body. The connecting column is provided with a fitting through-hole extending along its axis and penetrating it. The core body is slidably fitted inside the fitting through-hole.

[0021] Furthermore, a notch is provided on the side wall of the connecting column. The notch penetrates from the inner side wall of the connecting column to its outer side wall.

[0022] The notch extends along the axis of the connecting column from the end close to the first mesh plate towards the second mesh plate. A stirring rod is fixedly connected to the core body. The stirring rod extends through the notch to between the first mesh plate and the second mesh plate.

[0023] Furthermore, a stop post is fixedly connected to the side of the sealing block away from the extension tube, and the stop post extends along the axial direction of the liquid replenishing tube. A floating plate is disposed in the liquid replenishing tube.

[0024] When the liquid level in the liquid replenishing tube is lower than the stop post, the floating plate fits against the stop post. When the airflow pushes up the weight sealing member, the drainage needle pushes open the valve and lifts the floating plate, so that when the drainage needle resets, the floating plate can strike the stop post to emit a prompt sound.

[0025] A method for evaluating the cold resistance of Pennisetum alopecuroides based on spectral analysis includes the following steps:

[0026] S1. Prepare the Pennisetum alopecuroides detection sample.

[0027] S2. Perform low-temperature treatment on the Pennisetum alopecuroides detection sample, and detect the low-temperature exudation concentration of potassium ions by atomic absorption spectrometry in a solvent with a preset dosage.

[0028] S3. Place the Pennisetum alopecuroides detection sample processed in S2 into the receiving tube of the above cold resistance detection device, add a solvent with a preset dosage to the receiving tube, and add a supplementary solvent to the liquid replenishing tube.

[0029] S4. Pass a cooling medium into the reflux condenser tube, heat the receiving tube and make the solvent boil for a preset duration. After the heat treatment is completed, replenish the solvent volume in the receiving tube to the preset dosage, and detect the high-temperature exudation concentration of potassium ions by atomic absorption spectrometry.

[0030] S5. Determine the low-temperature exudation rate of potassium ions based on the low-temperature exudation concentration and the high-temperature exudation concentration, and evaluate the cold resistance of the Pennisetum alopecuroides detection sample according to the low-temperature exudation rate.

[0031] Furthermore, the method for evaluating the cold resistance of Pennisetum alopecuroides based on spectral analysis further includes the step: S6. Perform low-temperature treatment on the Pennisetum alopecuroides detection sample, obtain the cell activity data of the Pennisetum alopecuroides detection sample by Raman spectroscopy, and evaluate the cold resistance of the Pennisetum alopecuroides detection sample according to the cell activity data.

[0032] The beneficial effects of the technical solution of the embodiment of the present invention include:

[0033] During the use of the cold resistance detection device provided by the embodiment of the present invention, when the heating efficiency increases due to fluctuations, the amount of steam generated per unit time will increase, which may exceed the condensation efficiency of the reflux condenser tube, resulting in a part of the steam directly pushing up the weight sealing member. This part of the steam finally discharges through the exhaust port of the extension tube, which facilitates balancing the air pressure in the receiving tube and ensures safety.

[0034] Meanwhile, since a part of the steam is discharged from the steam discharge port, a part of the solvent is lost in the receiving pipe. When the air flow lifts the weight plugging member, the core body can push open the valve. The solvent in the liquid replenishing pipe is guided by the control core and discharged, and finally drained along the core body into the receiving pipe to replenish the solvent, further preventing the solvent from being accidentally dried out. After the excess steam is discharged, the weight plugging member resets under the action of gravity.

[0035] Generally speaking, the cold resistance detection device provided by the embodiment of the present invention can effectively reduce the probability of accidental drying of the solvent while realizing the detection with a relatively small amount of detection samples and a relatively small amount of solvent, ensuring the efficient and smooth progress of the detection work. The cold resistance evaluation method of Pennisetum based on spectral analysis provided by the embodiment of the present invention realizes the small consumption, high efficiency and low risk of the detection process at the same time, ensuring the efficient and smooth progress of the detection work. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the embodiments. It should be understood that the following drawings only show some embodiments of the present invention, and therefore should not be regarded as limiting the scope. For those of ordinary skill in the art, other related drawings can be obtained based on these drawings without creative efforts.

[0037] Figure 1 It is a schematic diagram of the overall structure of the cold resistance detection device provided by the embodiment of the present invention (when the weight plugging member is not lifted);

[0038] Figure 2 For Figure 1 It is a schematic diagram of the structure of area A in

[0039] Figure 3 For Figure 1 It is a schematic diagram of the structure of area B in

[0040] Figure 4 It is a schematic diagram of the overall structure of the cold resistance detection device provided by the embodiment of the present invention (when the weight plugging member is lifted);

[0041] Figure 5 For Figure 4 It is a schematic diagram of the structure of area C in

[0042] Figure 6 For Figure 4 It is a schematic diagram of the structure of area D in

[0043] Description of the reference numerals:

[0044] Receiving tube 100; Radial rod 110; Fitting column 120; Reflux condenser tube 200; Extension tube 300; Drain port 310; Liquid replenishing tube 400; Sealing block 410; Communication hole 411; Valve 412; Stopping column 420; Floating plate 430; Core body 510; Weight plugging member 520; Drainage needle 530; Stirring rod 540; Isolation member 600; First mesh plate 610; Second mesh plate 620; Connecting column 630; Notch 640. Detailed implementation manner

[0045] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. Usually, the components of the embodiments of the present invention described and illustrated in the accompanying drawings here can be arranged and designed in various different configurations.

[0046] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed present invention, but merely represents selected embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts fall within the scope of protection of the present invention.

[0047] It should be noted that: Similar reference numerals and letters denote similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0048] The terms "first", "second", etc. are only used for distinguishing descriptions and cannot be understood as indicating or implying relative importance.

[0049] In addition, the terms "parallel", "perpendicular", etc. do not mean that the components are required to be absolutely parallel or perpendicular, but can be slightly inclined. For example, "parallel" only means that its direction is more parallel relative to "perpendicular", and does not mean that the structure must be completely parallel, but can be slightly inclined.

[0050] In the description of the present invention, it should also be noted that, unless otherwise clearly defined and limited, the terms "set", "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected, or indirectly connected through an intermediate medium, and can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0051] To solve the deficiencies existing in the prior art, please refer to Figures 1 to 6, this embodiment provides a cold resistance detection device, which includes: a containing tube 100, a reflux condenser 200, an extension tube 300, a liquid supplement tube 400, and a control core.

[0052] The containing tube 100 is used to contain the test sample and the solvent. The solvent can be deionized water.

[0053] The containing tube 100 is communicated with the reflux condenser 200, and the reflux condenser 200 is arranged along the axial direction of the containing tube 100. The extension tube 300 is connected to one end of the reflux condenser 200 far away from the containing tube 100 and is communicated with the reflux condenser 200. The extension tube 300 is arranged along the axial direction of the reflux condenser 200. An evacuation port 310 is opened on the side wall of the extension tube 300.

[0054] One end of the liquid supplement tube 400 is closed by a sealing block 410. One end of the extension tube 300 far away from the reflux condenser 200 is connected to the sealing block 410, and the liquid supplement tube 400 is arranged along the axial direction of the extension tube 300.

[0055] The sealing block 410 is provided with a communication hole 411 that communicates the liquid supplement tube 400 and the extension tube 300. One end of the communication hole 411 far away from the extension tube 300 is fitted with a valve flap 412. When the liquid supplement tube 400 contains the solvent, the valve flap 412 is closed.

[0056] The control core includes a core body 510 and a weight plugging member 520. The core body 510 penetrates through the weight plugging member 520 and is fixedly connected to the weight plugging member 520. The diameter of the core body 510 is smaller than the inner diameter of the reflux condenser 200. One end of the core body 510 is fitted inside the reflux condenser 200, and the other end of the core body 510 is fitted into the communication hole 411. The weight plugging member 520 is located inside the extension tube 300.

[0057] In the natural state, the weight plugging member 520 abuts against one end of the reflux condenser 200 far away from the containing tube 100, thereby blocking the reflux condenser 200.

[0058] When the air flow lifts the weight plugging member 520, the core body 510 pushes open the valve flap 412.

[0059] The cold resistance detection device is used to heat the test sample at a high temperature so as to inactivate the cells of the test sample, thereby fully releasing potassium ions. In this way, by combining the potassium ion release concentration of the test sample after low-temperature treatment, the potassium ion release rate of the test sample after low-temperature treatment can be obtained, so as to be used to evaluate the cold resistance of the test sample.

[0060] During the use of the cold resistance detection device, when the solvent (taking deionized water as an example) in the containing tube 100 is heated and kept boiling, the water vapor condenses in the reflux condenser 200 and flows back into the containing tube 100, which can effectively reduce the volume loss and lower the probability of accidental drying of the solvent.

[0061] Since the cold resistance detection device is mainly for small-scale detection tests, the overall volume of the cold resistance detection device can be made relatively small. The volumes of the containing tube 100, the reflux condenser 200, the extension tube 300, and the liquid supplement tube 400 are all relatively small, reducing the requirement for the flow rate of the cooling medium. Since the amounts of the test sample and the solvent in the containing tube 100 are both small, and when heating the containing tube 100, the heating efficiency cannot be completely stable and unchanged, the heating efficiency may fluctuate within a certain range. Therefore, the amount of solvent vapor is also fluctuating. When the heating efficiency increases due to fluctuations, the amount of vapor generated per unit time will increase, which may exceed the condensation efficiency of the reflux condenser 200, causing a part of the vapor to directly push up the weight plug 520. This part of the vapor is finally discharged through the exhaust port 310 of the extension tube 300, which is convenient for balancing the air pressure in the containing tube 100 and ensuring safety.

[0062] At the same time, since a part of the vapor is discharged through the exhaust port 310, a part of the solvent is lost in the containing tube 100. When the air flow pushes up the weight plug 520, the core 510 can push open the valve 412. The solvent in the liquid supplement tube 400 is guided by the control core and is finally drained along the core 510 into the containing tube 100 to achieve the replenishment of the solvent and further prevent the solvent from being accidentally dried. After the excess vapor is discharged, the weight plug 520 resets under the action of gravity.

[0063] It can be understood that the amount of solvent replenished from the liquid supplement tube 400 when the control core pushes open the valve 412 once can be controlled by adjusting the weight of the weight plug 520 and the aperture of the communication hole 411. Optionally, the amount of solvent replenished from the liquid supplement tube 400 when the control core pushes open the valve 412 once can be set to be less than or equal to the amount of solvent corresponding to the vapor discharged when the weight plug 520 is pushed up.

[0064] Generally speaking, the cold resistance detection device provided in this embodiment can effectively reduce the probability of accidental drying of the solvent while realizing the detection with a relatively small amount of test sample and a relatively small amount of solvent, ensuring the efficient and smooth progress of the detection work.

[0065] In this embodiment, the control core further includes: a drainage needle 530.

[0066] The core body 510 has an axial through-hole extending along its axis and penetrating it. The drainage needle 530 is accommodated in the core body 510 and is arranged to extend along the axis of the core body 510. The diameter of the drainage needle 530 is smaller than the inner diameter of the core body 510, and the drainage needle 530 is coaxially and fixedly connected to the core body 510. Optionally, the drainage needle 530 can be connected to the inner wall of the core body 510 through a connecting wire (not shown in the figure), and the diameter of the connecting wire is smaller than or equal to the diameter of the drainage needle 530.

[0067] The outer diameter of the core body 510 is adapted to the aperture of the communication hole 411, and the core body 510 is slidably fitted in the communication hole 411. The drainage needle 530 extends beyond the end of the core body 510 away from the receiving tube 100. When the air flow pushes up the weight plugging member 520, the core body 510 pushes open the valve 412 through the drainage needle 530. In this way, the solvent in the liquid replenishing tube 400 is finally replenished into the receiving tube 100 under the guiding action of the drainage needle 530 through the axial through-hole of the core body 510. Through this design, the smoothness of solvent replenishment is improved, and the loss of solvent during replenishment is reduced.

[0068] Further, a radial rod 110 is fixedly connected inside the receiving tube 100, the radial rod 110 is fixedly connected with a fitting column 120, the fitting column 120 is coaxially arranged with the core body 510, and the diameter of the fitting column 120 is adapted to the inner diameter of the core body 510. The core body 510 extends into the receiving tube 100, and the fitting column 120 is fitted in the core body 510.

[0069] Among them, when the air flow pushes up the weight plugging member 520, the core body 510 moves towards the liquid replenishing tube 400 and separates from the fitting column 120. That is to say, when the weight plugging member 520 is not pushed up, the fitting column 120 is fitted in the core body 510, thereby closing the lower end of the core body 510 to prevent steam from being lost through the axial through-hole of the core body 510. When the air flow pushes up the weight plugging member 520, the core body 510 separates from the fitting column 120, and the lower end of the core body 510 is opened, which facilitates the drainage needle 530 to smoothly replenish the solvent into the mixing system of the receiving tube 100.

[0070] Specifically, a separator 600 is further provided inside the receiving tube 100. The separator 600 includes a first mesh plate 610, a connecting column 630, and a second mesh plate 620.

[0071] Both the first mesh plate 610 and the second mesh plate 620 are arranged perpendicular to the axis of the receiving tube 100 and are spaced apart from each other. The first mesh plate 610 is located on the side of the second mesh plate 620 closer to the bottom of the receiving tube 100, and the connecting column 630 fixedly connects the first mesh plate 610 and the second mesh plate 620.

[0072] The separator 600 is detachably installed inside the receiving tube 100, and the separator 600 is located on the side of the mating post 120 away from the bottom of the receiving tube 100. The space between the first mesh plate 610 and the second mesh plate 620 is used to hold the test sample, thus preventing the test sample from adhering to the bottom of the receiving tube 100, thereby reducing the possibility of the test sample being burnt due to adhesion.

[0073] The connecting post 630 is coaxially arranged with the core body 510. The connecting post 630 is provided with a mating through hole that runs through it along its axial direction. The outer diameter of the core body 510 is adapted to the aperture of the mating through hole, and the core body 510 is slidably fitted inside the mating through hole.

[0074] A notch 640 is provided on the side wall of the connecting post 630. The notch 640 runs from the inner side wall of the connecting post 630 to its outer side wall.

[0075] The notch 640 extends along the axial direction of the connecting post 630 from the end close to the first mesh plate 610 towards the second mesh plate 620. The core body 510 is fixedly connected with a stirring rod 540. The stirring rod 540 extends through the notch 640 to the space between the first mesh plate 610 and the second mesh plate 620. In this way, when the airflow lifts the weight plug 520, the core body 510 will also drive the stirring rod 540 to move simultaneously, thereby stirring the test sample between the first mesh plate 610 and the second mesh plate 620, which helps the test sample to fully release potassium ions and reduces the possibility of the test sample blocking the mesh holes of the first mesh plate 610 and the second mesh plate 620.

[0076] Furthermore, a stop post 420 is fixedly connected to the side of the sealing block 410 away from the extension tube 300. The stop post 420 extends along the axial direction of the liquid supply tube 400. A floating plate 430 is disposed inside the liquid supply tube 400. The density of the floating plate 430 is lower than the density of the solvent.

[0077] When the liquid level in the liquid supply tube 400 is lower than the stop post 420, the floating plate 430 fits against the stop post 420. When the airflow lifts the weight plug 520, the drainage needle 530 pushes open the valve 412 and lifts the floating plate 430, so that when the drainage needle 530 resets, the floating plate 430 can strike the stop post 420 to emit a prompt sound, which is used to prompt the staff to pay attention to the stock of the solvent, thus playing a reminder role and preventing the solvent from being accidentally dried out due to the staff's forgetting.

[0078] In addition, the floating plate 430 can reduce the oscillation amplitude of the solvent liquid level in the liquid supply tube 400. During the process of the weight plug 520 being repeatedly lifted, the solvent in the liquid supply tube 400 can be prevented from being sloshed out from the mouth of the liquid supply tube 400.

[0079] In this embodiment, the weight plug 520 is arranged as a sphere, and the weight plug 520 is fixedly connected to the core body 510 coaxially.

[0080] This embodiment also provides a method for evaluating the cold resistance of Pennisetum alopecuroides based on spectral analysis. The method includes the following steps:

[0081] S1. Prepare Pennisetum alopecuroides detection samples.

[0082] S2. Perform low-temperature treatment on the Pennisetum alopecuroides detection samples. After the low-temperature treatment, fully release potassium ions from the Pennisetum alopecuroides detection samples with a preset dosage of solvent, and detect the low-temperature exudation concentration of potassium ions by atomic absorption spectrometry.

[0083] S3. Wash and dry the Pennisetum alopecuroides detection samples after being treated in S2, and place them into the receiving tube 100 of the above-mentioned cold resistance detection device. Then add the same preset dosage of solvent as in S2 to the receiving tube 100, and add supplementary solvent to the liquid replenishing tube 400.

[0084] S4. Pass a cooling medium into the reflux condenser 200, heat the receiving tube 100 and make the solvent boil for a preset duration. After the heat treatment, replenish the solvent volume in the receiving tube 100 to the preset dosage, and detect the high-temperature exudation concentration of potassium ions by atomic absorption spectrometry.

[0085] S5. Determine the low-temperature exudation rate of potassium ions according to the low-temperature exudation concentration and the high-temperature exudation concentration, and evaluate the cold resistance of the Pennisetum alopecuroides detection samples according to the low-temperature exudation rate.

[0086] S6. Perform low-temperature treatment on the Pennisetum alopecuroides detection samples, obtain the cell activity data of the Pennisetum alopecuroides detection samples by Raman spectroscopy, and evaluate the cold resistance of the Pennisetum alopecuroides detection samples according to the cell activity data.

[0087] Combining the results of S5 and S6, realize the multi-spectral comprehensive analysis of the cold resistance of the Pennisetum alopecuroides detection samples.

[0088] In summary, the cold resistance detection device provided by the embodiment of the present invention can realize the detection with a relatively small amount of detection samples and a relatively small amount of solvent, effectively reduce the probability of accidental drying of the solvent, and ensure the efficient and smooth progress of the detection work. The method for evaluating the cold resistance of Pennisetum alopecuroides based on spectral analysis provided by the embodiment of the present invention also realizes small dosage, high efficiency and low risk in the detection process, and ensures the efficient and smooth progress of the detection work.

[0089] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cold resistance detection device, characterized in that: include: A containing tube, a reflux condenser tube, an extension tube, a liquid replenishing tube and a control core; The containing tube is used to contain the test sample and the solvent; The receiving tube is connected to the reflux condensing tube, the extension tube is connected to one end of the reflux condensing tube away from the receiving tube and is connected to the reflux condensing tube; a drain port is provided on the side wall of the extension tube; one end of the infusion tube is closed by a sealing block, and one end of the extension tube away from the reflux condensing tube is connected to the sealing block; the sealing block is provided with a connecting hole connecting the infusion tube and the extension tube, and a valve is provided at one end of the connecting hole away from the extension tube; when the infusion tube contains the solvent, the valve is closed; The control core comprises a core body and a counterweight plugging piece, the core body passes through the counterweight plugging piece and is fixedly connected to the counterweight plugging piece; the diameter of the core body is smaller than the inner diameter of the reflux condenser tube, one end of the core body is fitted into the reflux condenser tube, the other end of the core body is fitted into the connecting hole, and the counterweight plugging piece is located in the extension tube; in a natural state, the counterweight plugging piece abuts against one end of the reflux condenser tube away from the receiving tube, thereby plugging the reflux condenser tube; When the airflow pushes up the counterweight blocking member, the core pushes open the valve; The control core further includes: a drainage needle; The core body has an axial through hole extending along the axial direction thereof and penetrating the core body, and the drainage needle is accommodated in the core body and is arranged to extend along the axial direction of the core body; the diameter of the drainage needle is smaller than the inner diameter of the core body, and the drainage needle is fixedly connected to the core body; The drainage needle extends to the outside of one end of the core body away from the accommodating tube; when the airflow pushes up the counterweight blocking member, the drainage needle pushes open the valve; A radial rod is fixedly connected inside the receiving tube, and a matching column is fixedly connected to the radial rod. The matching column is coaxially arranged with the core body, and the diameter of the matching column is adapted to the inner diameter of the core body; the core body extends into the receiving tube, and the matching column is matched in the core body; When the airflow lifts up the counterweight sealing member, the core moves toward the fluid infusion tube and separates from the matching column.

2. The cold resistance detection device according to claim 1, characterized in that: The containing tube is provided with an isolating member, and the isolating member comprises a first mesh plate, a connecting column and a second mesh plate; The first mesh plate and the second mesh plate are both arranged perpendicular to the axis of the containing tube and are spaced apart from each other. The first mesh plate is located on a side of the second mesh plate close to the bottom of the containing tube, and the connecting column fixedly connects the first mesh plate and the second mesh plate. The isolating member is detachably mounted in the containing tube, and the isolating member is located on a side of the matching column away from the bottom of the containing tube; The connecting column is coaxially arranged with the core body, and the connecting column is provided with a matching through hole which is arranged along the axial direction thereof and penetrates the connecting column, and the core body is slidably fitted in the matching through hole.

3. The cold resistance detection device according to claim 2, characterized in that: A notch is formed on the side wall of the connecting column, and the notch extends from the inner side wall of the connecting column to the outer side wall thereof; The notch extends from one end of the connecting column close to the first mesh plate along its axial direction toward the second mesh plate; the core body is fixedly connected with a stirring rod, and the stirring rod extends from the notch to between the first mesh plate and the second mesh plate.

4. The cold resistance detection device according to claim 1, characterized in that: A stop column is fixedly connected to one side of the sealing block away from the extension tube, and the stop column extends along the axial direction of the infusion tube; a floating plate is arranged inside the infusion tube; When the liquid level in the infusion tube is lower than the stop post, the float plate fits against the stop post. When the airflow lifts the counterweight sealing member, the drainage needle pushes open the valve and lifts the float plate, so that when the drainage needle is reset, the float plate can hit the stop post to emit a prompt sound.

5. A method for evaluating the cold resistance of Pennisetum based on spectral analysis, characterized in that: The steps include: S1. Prepare Pennisetum test samples; S2, subjecting the Pennisetum test sample to low temperature treatment, and detecting the low temperature seepage concentration of potassium ions by atomic absorption spectrometry in a preset amount of solvent; S3, placing the Pennisetum test sample processed by S2 into the receiving tube of the cold resistance testing device according to any one of claims 1 to 4, adding the preset amount of the solvent into the receiving tube, and adding the solvent for replenishment into the liquid infusion tube; S4, introducing a cooling medium into the reflux condenser, heating the receiving tube and causing the solvent to boil for a preset time, replenishing the amount of solvent in the receiving tube to the preset amount after the heat treatment is completed, and detecting the high-temperature seepage concentration of potassium ions by atomic absorption spectroscopy; S5. Determine the low-temperature seepage rate of potassium ions according to the low-temperature seepage concentration and the high-temperature seepage concentration, and evaluate the cold resistance of the Pennisetum test sample according to the low-temperature seepage rate.

6. The method for evaluating the cold resistance of Pennisetum truncatum based on spectral analysis according to claim 5, characterized in that: The following steps are also included: S6. Perform low-temperature treatment on the Pennisetum test sample, obtain cell activity data of the Pennisetum test sample using Raman spectroscopy, and evaluate the cold resistance of the Pennisetum test sample based on the cell activity data.

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