Lily living body seed ball breeding method

Abstract

The method specifically comprises the following steps that small lily bulbs are planted, 3-4 weeks after budding, an amino acid water-soluble fertilizer containing forchlorfenuron or thidiazuron is used for spraying seedlings, after two months of lily flowering, bulbils growing on plant stalks are mature, the plant stalks are cut off from the roots, and the bulbils grow on the plant stalks. Cutting into small sections according to a planting vessel, burying into nutrient soil, watering and culturing. According to the method for breeding the lily bulbs in vivo, a traditional mode is broken through, another way is opened, plant stalks obtain bulbils through exogenous regulation and control to produce the lily bulbs, the breeding cost of the lily bulbs is greatly reduced, and the method plays a good role in promoting the lily seed industry in China.

Description

Technical Field

[0001] The invention relates to the field of garden plant cultivation and maintenance, in particular to a method for living propagation of lily seed bulbs. Background Art

[0002] As a world-famous bulb flower and one of the five major fresh cut flowers, lily has a very broad development prospect in the flower industry due to its multiple values ​​of medicine, appreciation and food. The development of the lily industry and the continuous expansion of market demand have put forward higher requirements on the quality and quantity of lily bulbs. my country's lily seed industry is basically at the starting stage.

[0003] Bulbula is a form of reproduction in lily, an above-ground organ that grows in axillary buds and originates from axillary meristems, similar to tillers in Arabidopsis and rice. Bulbula and bulbil are essentially the same, both are reproductive organs of lily, but they are in different locations. Bulbs grow in the underground part, while bulbils grow in the above-ground part. When the bulbils mature and fall to the ground, they begin to develop into bulbs. However, not all Lilium species can form bulbils. Currently, four wild lily species, namely Lilium lancifolium, Lilium sulphureum, Lilium sargentiae and Lilium bulbiferum, are known to naturally form bulbils in the axillary buds in the stems, but this method of reproduction is only applicable to some Lilium materials where bulbils can occur.

[0004] Obtaining bulbs through scale cuttings is currently the main method of lily reproduction, but the price of lily bulbs has remained high and is increasing year by year, causing the cost of reproduction to increase dramatically. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a method for living propagation of lily seed bulbs which has low cost, simple operation and excellent effect.

[0006] The method for living propagation of lily bulbs of the present invention comprises the following steps:

[0007] Plant small lily bulbs and spray the seedlings with amino acid water-soluble fertilizer containing chlorforuzonium or thidiazuron 3-4 weeks after germination. Wait for 2 months after the lilies bloom and the bulbils growing on the plant stems mature. Cut the plant stems from the roots, cut them into small sections according to the planting container, bury them in nutrient soil, and water them for cultivation.

[0008] The method for living propagation of lily bulbs of the present invention comprises the following steps: the dilution ratio of the amino acid water-soluble fertilizer is 1:1000, chlorforcarbamide or thidiazuron is added after dilution, the volume ratio of chlorforcarbamide to the diluted amino acid water-soluble fertilizer is 1:50, and the volume ratio of thidiazuron to the diluted amino acid water-soluble fertilizer is 1:50.

[0009] The method for living propagation of lily seed bulbs of the present invention comprises the following steps: the circumference of the lily small seed bulbs is 6-8 cm, the lily small seed bulbs are cultured in a greenhouse, watered normally, and sprayed 3-4 weeks after germination, wherein the spraying is foliar spraying.

[0010] The method for living propagation of lily bulbs of the present invention comprises spraying again at an interval of 3 weeks after spraying, and waiting for two months after the lily blooms for the bulbils grown from the plant stems to mature.

[0011] The method for living propagation of lily seed bulbs of the present invention comprises the following steps: after the bulbils mature, the plant stems are cut off from the roots; during planting, the stems with the bulbils are cut into stem segments of 7-10 cm, which are horizontally placed in nutrient soil and covered with 2-3 cm of nutrient soil; watering is performed using water containing rooting powder to promote the rooting of the bulbils; after 2 weeks, the bulbils grow roots; after 1 month, the bulbils can grow scale leaves; and after 1-2 years of planting, the bulbils grow into lily seed bulbs.

[0012] The method for living propagation of lily bulbs of the present invention is different from the prior art in that:

[0013] The method for living propagation of lily bulbs of the present invention breaks through the traditional way and takes a new approach. Through exogenous regulation, bulbils are obtained from plant stems to produce lily bulbs, thereby greatly reducing the propagation cost of lily bulbs, which will play a good role in promoting my country's lily seed industry.

[0014] The method of the present invention can be used to harvest stalk bulbils and bulbs, and the size and weight of the bulbils are no different from those of the control, indicating that the nutrients and energy consumed by the growth of the bulbils can be compensated by the use of amino acid water-soluble fertilizers, indicating that the method of the present invention can obtain bulbils for lily reproduction without affecting the original yield of the lily.

[0015] The method for living propagation of lily bulbs of the present invention will be further described below in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The lily plant seedlings grown for 3-4 weeks in the embodiment of the present invention;

[0017] Figure 2 The bulbils grow in the axils of the stems of lily plants in the embodiment of the present invention, wherein: CK: untreated; 1: amino acid water-soluble fertilizer; 2: amino acid aqueous solution + chlorfenapyr; 3: amino acid water-soluble fertilizer + thidiazuron;

[0018] Figure 3 To obtain the statistical results of lily bulb harvest using the method of the present invention;

[0019] Figure 4The planting diagram and growth diagram of lily bulbils in the method of the present invention are shown in the figure below: left: cut stem segment; middle: bulbils taking root; right: bulbils growing scale leaves;

[0020] Figure 5 The diagram shows the effects of spraying amino acid water-soluble fertilizer + chlorfenapyr on some series of lily varieties using the method of the present invention. In the diagram, the upper row from left to right are Pol Jessica, Losera's Dream, Scheherazade, Isabella and Zambezi; the lower row from left to right are Pink Flight, Tiger Baby, Two-Player Game, Vanilla Flavour and Comfortable Waltz. DETAILED DESCRIPTION

[0021] 1. Experimental Materials

[0022] Lily bulbs, amino acid water-soluble fertilizer, chlorforamide, thidiazuron, 'Ruqing' brand substrate nutrient soil.

[0023] 2. Experimental methods

[0024] Plant the 'Shuttle' lily bulbs (6-8cm in diameter) in nutrient soil, cultivate in the greenhouse, manage and water normally, and 3-4 weeks after germination ( Figure 1 ), use amino acid water-soluble fertilizer containing chlorfenapyr or thidiazuron to spray the seedlings on the leaves (it is necessary to ensure that the whole plant is sprayed); among them, the dilution volume ratio of the amino acid water-soluble fertilizer is 1:1000, and chlorfenapyr or thidiazuron is added after dilution. The volume ratio of chlorfenapyr and the diluted amino acid water-soluble fertilizer is 1:50, and the volume ratio of thidiazuron and the diluted amino acid water-soluble fertilizer is 1:50. Spraying can be carried out again after an interval of 3 weeks. After 2-3 months of flowering, the bulbils grown on the plant stems are mature and can take root normally. Cut the plant stems from the roots and cut them into 7-10cm stem segments according to the planting container. Place them horizontally in the nutrient soil and cover them with nutrient soil with a thickness of 3-4cm. Use water containing rooting powder for watering to promote the normal rooting and germination of the bulbils in the soil.

[0025] 3. Experimental results

[0026] 3.1 Effects of amino acid water-soluble fertilizer, chlorfenapyr and thidiazuron on the growth of lily bulbils

[0027] Three weeks after lily bulbs sprouted, 1# (amino acid water-soluble fertilizer), 2# (amino acid water-soluble fertilizer + chlorfenapyr), and 3# (amino acid water-soluble fertilizer + thidiazuron) were used for foliar spraying. Among them, 2# and 3# containing chlorfenapyr and thidiazuron began to sprout bulbils in the axils of the stems 3-4 weeks after treatment ( Figure 2 ), the growth of bulbils will delay the flowering of the plant by about 1 week. The statistical results of bulb harvest are shown in the table and Figure 3The results showed that treatment 1# could significantly increase the yield of bulbs, and the size and circumference of the bulbs were significantly increased. Both treatments 2# and 3# could harvest bulbils and bulbs from the stalks, and the size and weight of the bulbs were no different from those of the control. This result showed that the nutrients and energy consumed by the growth of bulbils could be compensated by the use of amino acid water-soluble fertilizers. Therefore, this method can be used to obtain bulbils for lily reproduction without affecting the original yield of lilies.

[0028] Table 1. Effects of amino acid water-soluble fertilizer, chlorfenapyr and thidiazuron on bulb weight

[0029]

[0030] Table 2. Effects of amino acid water-soluble fertilizer, chlorfenapyr and thidiazuron on bulb circumference

[0031]

[0032] 3.2 Planting of lily bulbils

[0033] After the lilies are treated with amino acid water-soluble fertilizer and chlorfenapyr or thidiazuron, the bulbils on the stems will grow for 2-3 months and can be used for planting. When planting, cut the stems with bulbils into 7-10 cm stem segments, place them horizontally in "Ruqing" brand nutrient soil, cover them with 3-4 cm of nutrient soil, and water them with water containing rooting powder to promote the rooting of the bulbils. After 2 weeks, the bulbils can grow roots, and after 1 month, the bulbils can grow scale leaves ( Figure 4 ), after 1-2 years of planting, it can grow into lily bulbs.

[0034] 3.3 Spraying effects of different lily varieties

[0035] The above experiments prove that both chlorfenapyr and thidiazuron can promote the germination of bulbils in the axils of stems when used in the lily seedling stage. This method has been tested on multiple lily varieties and can promote the formation of bulbils in the axils of stems ( Figure 5 ), with an efficiency of over 90%.

[0036] The specific lily series varieties are as follows:

[0037] Asiatic Lily: Rosella's Dream, Dance of the Tribe, Nitty's Pride, Bite Juan, Pol Jessica, Walker, Summer Sunshine, Lollipop, Happy Memories, The Best, Pink Morpho, Sheherazade;

[0038] Pollen-free series: Two-player game, Vanilla, Spotted, Cozy Waltz, Cozy Sunshine;

[0039] Spot series: Strawberry Banquet, Pink Flight, Tiger Baby;

[0040] Trumpet Series: White Planet;

[0041] Iron Cannon Series: Kali, Difus;

[0042] Oriental Series: Tuberose Mountain, Isabela, Editha, Siberia, Zambezi.

[0043] The embodiments described above are merely descriptions of preferred implementation modes of the present invention and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for living propagation of lily bulbs, characterized in that: The steps include: Plant small lily bulbs and spray the seedlings with amino acid water-soluble fertilizer containing chlorforuzonium or thidiazuron 3-4 weeks after germination. Wait for 2 months after the lilies bloom and the bulbils growing on the plant stems mature. Cut the plant stems from the roots, cut them into small sections according to the planting container, bury them in nutrient soil, and water them for cultivation.

2. The method for living propagation of lily bulbs according to claim 1, characterized in that: The dilution volume ratio of the amino acid water-soluble fertilizer is 1:1000, and chlorfenapyr or thidiazuron is added after dilution, the volume ratio of chlorfenapyr and the diluted amino acid water-soluble fertilizer is 1:50, and the volume ratio of thidiazuron and the diluted amino acid water-soluble fertilizer is 1:

50.

3. The method for living propagation of lily bulbs according to claim 1, characterized in that: The lily bulblets have a circumference of 6-8 cm, are cultured in a greenhouse, are watered normally, and are sprayed 3-4 weeks after germination, wherein the spraying is foliar spraying.

4. The method for living propagation of lily bulbs according to claim 1, characterized in that: Spray again after 3 weeks. Two months after the lily blooms, the bulbils growing on the plant stems will mature.

5. The method for living propagation of lily bulbs according to claim 1, characterized in that: After the bulbils mature, cut the plant stems from the roots. When planting, cut the stems with bulbils into 7-10 cm stem segments, place them horizontally in the nutrient soil, cover them with 3-4 cm of nutrient soil, and water them with water containing rooting powder to promote the rooting of the bulbils. After 2 weeks, the bulbils will grow roots, and after 1 month, the bulbils can grow scale leaves. After 1-2 years of planting, they will grow into lily bulbs.

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