Preparation method of nanocarbon-coated titanium dioxide particles and application of nanocarbon-coated titanium dioxide particles in prevention and treatment of fungal diseases of rice

Abstract

The invention discloses a preparation method of nanocarbon-coated titanium dioxide particles. The preparation method comprises the following steps: adding a carbon source into a titanium dioxide precursor solution, and preparing a carbon-coated titanium dioxide nanomaterial by adopting an in-situ solvothermal method, the preparation process is simple, and high-temperature calcination is not needed. The absorption range of the carbon-coated titanium dioxide is expanded from ultraviolet light to visible light, so that the utilization rate of sunlight is improved. The invention further discloses application of the nanocarbon-coated titanium dioxide in preventing and treating fungal diseases of rice, which comprises the following steps: adding the carbon-coated titanium dioxide nanomaterial with a certain concentration into a culture medium, and culturing pathogens of the fungal diseases of rice under visible light for 24-48 hours. The nanocarbon-coated titanium dioxide particles can significantly inhibit the growth of pathogens of fungal diseases of rice under the irradiation of visible light, and provide reference for the development of novel nano pesticide preparations and agricultural application.

Description

Technical Field

[0001] The present invention relates to the technical field of nano materials and plant protection, and more specifically, to a preparation method of nano carbon-coated titanium dioxide particles and application of the nano carbon-coated titanium dioxide particles in preventing and controlling rice fungal diseases. Background Art

[0002] Rice is an important food crop in my country, and its disease prevention and control has always been an important task in my country's agricultural development. Among them, rice fungal diseases are of various types and serious harm, posing a huge threat to rice yield and quality, and are currently an urgent problem to be solved in my country's agriculture. There are more than 10 common fungal diseases of rice, and the main and more serious ones are rice sheath blight, seedling blight, rice false smut and rice blast. The use of photocatalytic antibacterial agents can effectively inhibit the growth of fungal disease pathogens, achieve efficient utilization of light energy, and have fewer side effects than other methods such as disinfectants and antibiotics.

[0003] Titanium dioxide is a type of material that has attracted much attention in the research of antibacterial applications of many photocatalytic materials. Matsunaga et al. (Tadashi M, Ryozo T, Toshiaki N, et al., Photoelectrochemical sterilization of microbial cells by semiconductor powders [J]. FEMS Microbiology Letters, 1985, 29, 211–214.) first used platinum-modified titanium dioxide photocatalysts to kill yeast, Lactobacillus acidophilus and Escherichia coli in water, and explained the sterilization mechanism, proving that titanium dioxide photocatalysts have excellent antibacterial properties. It is generally believed that its mechanism of action is: titanium dioxide, as an N-type semiconductor transition metal oxide, can absorb ultraviolet light to generate photogenerated carriers. After migrating to the surface of nanoparticles, the photogenerated carriers combine with donors or acceptors (for example, forming reactive oxygen species) to participate in redox reactions, or directly react with microorganisms to achieve an antibacterial effect. Pantaroto et al (Pantaroto HN, Ricomini-Filho AP, Bertolini MM, et al. Antibacterial photocatalytic activity of different crystalline TiO 2phases in oral multispecies biofilm[J].DentalMaterials.2018,34,E182–E195.) deposited titanium dioxide film on commercial pure titanium and observed its antibacterial effect. After 1 hour of ultraviolet light irradiation, the number of bacteria on the surface of titanium dioxide film decreased by about 99%, indicating that titanium dioxide film exhibited significant antibacterial activity after ultraviolet light irradiation. In order to prevent the recombination of photogenerated carriers and extend the photoactivity to the range of the solar spectrum, Gomez-Polo et al. (Gomez-Polo C, Larumbe S, Gil A, et al. Improved photocatalytic and antibacterial performance of Cr doped TiO 2 nanoparticles[J].Surface and Interface Analysis.2021,22,100867.) used chromium to modify titanium dioxide photocatalysts and proved that chromium is evenly distributed in titanium dioxide photocatalysts, which can promote the absorption spectrum of titanium dioxide to shift from ultraviolet light to visible light. The antibacterial ability of chromium-modified titanium dioxide photocatalysts under visible light can reach more than 200 times that under dark conditions. However, metallic chromium is a heavy metal and is not suitable for large-scale agricultural applications to prevent and control rice fungal diseases. Summary of the invention

[0004] The first technical problem to be solved by the present invention is to provide a method for preparing nano-carbon-coated titanium dioxide particles. The present invention adopts an in-situ solvothermal method to carbon-coat titanium dioxide, and the prepared nano-carbon-coated titanium dioxide particles can significantly inhibit the growth of rice fungal disease pathogens under visible light irradiation, thereby protecting rice from fungal diseases.

[0005] The second technical problem to be solved by the present invention is to provide an application of nano-carbon coated titanium dioxide particles in preventing and controlling rice fungal diseases.

[0006] In order to solve the above-mentioned first technical problem, the technical solution adopted by the invention is as follows:

[0007] A method for preparing nanocarbon-coated titanium dioxide particles comprises the following steps:

[0008] 1) dissolving a titanium dioxide precursor in ethanol to form a titanium dioxide precursor solution;

[0009] 2) adding acid to the titanium dioxide precursor solution;

[0010] 3) dissolving the carbon source in a mixed solvent of ethanol and water to form a mixed solution;

[0011] 4) slowly adding the mixed solution into the titanium dioxide precursor solution under stirring, and mixing evenly to form a transparent sol;

[0012] 5) transferring the transparent sol to a hydrothermal reactor with a polytetrafluoroethylene reaction liner and heating the reaction;

[0013] 6) After the heating reaction is completed, the reactor is cooled to room temperature to obtain a brown precipitate, which is then centrifuged, washed, and dried to obtain nano-carbon-coated titanium dioxide particles.

[0014] Preferably, in step 1), the titanium dioxide precursor is tetrabutyl titanate;

[0015] Preferably, in step 2), the acid is glacial acetic acid; the volume ratio of tetrabutyl titanate to the acid in the titanium dioxide precursor solution is 10-30:1; more preferably, the volume ratio of tetrabutyl titanate to the acid is 10:1.

[0016] Preferably, in step 3), the amount of water in the mixed solution is 12%-18% of the volume of tetrabutyl titanate; more preferably, the amount of water is 12% of the volume of tetrabutyl titanate.

[0017] Preferably, in step 3), the carbon source is glucose; the amount of the carbon source in the mixed solution is 1%-6% of the amount of tetrabutyl titanate; more preferably, the amount of the carbon source is 3% of the amount of tetrabutyl titanate.

[0018] Preferably, in step 4), the stirring speed is 500-1000 rpm

[0019] Preferably, in step 5), the heating reaction temperature is 150-200°C and the time is 10-20h; more preferably, the heating reaction temperature is 170°C and the time is 10h.

[0020] Preferably, in step 6), the centrifugal speed is 10000-15000 rpm, the washing is 3-5 times with anhydrous ethanol, the drying temperature is 55-65° C., and the drying time is 8-12 h.

[0021] To solve the above second technical problem, the present invention adopts the following technical solution :

[0022] An application of nano-carbon coated titanium dioxide particles in preventing and controlling rice fungal diseases comprises the following specific steps:

[0023] 11) Pick a small amount of mycelium from the frozen strain of rice fungal pathogens with a sterile inoculation needle and inoculate it in the middle of the PDA plate, and culture it in a constant temperature incubator at 28±1°C until the mycelium covers the entire plate, and set aside;

[0024] 12) dispersing the nanocarbon-coated titanium dioxide particles in deionized water to prepare a mother solution of a certain concentration;

[0025] 13) adding different volumes of mother solution to PDA culture medium to obtain poisoned PDA culture medium of different mass concentrations;

[0026] 14) Inoculate the bacterial cake (5 mm in diameter) into the poisonous plate and the blank control; place it in a constant temperature incubator at 28±1℃ with the light on for 24-48 hours, and then measure the colony diameter using the cross method.

[0027] Preferably, in step 11), the rice fungal disease pathogen is any one or more pathogens of rice sheath blight, rice blast, rice false smut and seedling bakanae.

[0028] Preferably, in step 13), the mass concentration of the poisonous PDA culture medium is 10-100 mg / L; more preferably, the mass concentration of the poisonous PDA culture medium is 10 mg / L.

[0029] Any range described in the present invention includes the end value and any numerical value between the end values ​​and any sub-range formed by the end value or any numerical value between the end values.

[0030] Unless otherwise specified, all raw materials in the present invention can be purchased from the market, and the equipment used in the present invention can adopt conventional equipment in the relevant field or refer to the existing technology in the relevant field.

[0031] Compared with the prior art, the present invention has the following beneficial effects: :

[0032] 1) The nanocarbon-coated titanium dioxide particles of the present invention break through the limitation that ordinary titanium dioxide particles mainly exert photocatalytic antibacterial effects under ultraviolet light, and can significantly inhibit the growth of rice fungal disease pathogens under visible light.

[0033] 2) The present invention uses green and non-toxic glucose as a carbon source and adopts an in-situ solvothermal method to prepare nano-carbon-coated titanium dioxide particles; the preparation process is simple and does not require high-temperature calcination; the absorption range of the carbon-coated titanium dioxide particles is expanded from ultraviolet light to visible light, thereby improving the utilization rate of sunlight; the nano-carbon-coated titanium dioxide particle material is used to cultivate rice fungal disease pathogens under visible light, which can significantly inhibit the growth of rice fungal disease pathogens, providing a reference for the development of new nano-pesticide antibacterial agents. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The specific embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0035] Figure 1 This is a TEM image of the nano-carbon-coated titanium dioxide prepared in Example 1;

[0036] Figure 2 The antibacterial results of different concentrations of nano-carbon-coated titanium dioxide particles against Rhizoctonia solani of Examples 1-3 of the present invention are shown;

[0037] Figure 3 The graph is a graph showing the antibacterial effect of nano-carbon-coated titanium dioxide particles on Rhizoctonia solani at different glucose dosages in Examples 7-9 of the present invention;

[0038] Figure 4 The graph is a graph showing the antibacterial effect of titanium dioxide particles of different concentrations on Rhizoctonia solani in Comparative Examples 1-3 of the present invention;

[0039] Figure 5 It is a graph showing the antibacterial results of comparative examples 4-6 of the present invention. DETAILED DESCRIPTION

[0040] In order to more clearly illustrate the present invention, the present invention is further described below in conjunction with preferred embodiments and accompanying drawings. Similar components in the accompanying drawings are represented by the same reference numerals. It should be understood by those skilled in the art that the content specifically described below is illustrative rather than restrictive, and should not be used to limit the scope of protection of the present invention.

[0041] As one aspect of the present invention, a method for preparing nanocarbon-coated titanium dioxide particles comprises the following steps:

[0042] 1) dissolving a titanium dioxide precursor in ethanol to form a titanium dioxide precursor solution;

[0043] 2) adding acid to the titanium dioxide precursor solution;

[0044] 3) dissolving the carbon source in a mixed solvent of ethanol and water to form a mixed solution;

[0045] 4) slowly adding the mixed solution into the titanium dioxide precursor solution under stirring, and mixing evenly to form a transparent sol;

[0046] 5) transferring the transparent sol to a hydrothermal reactor with a polytetrafluoroethylene reaction liner and heating the reaction;

[0047] 6) After the heating reaction is completed, the reactor is cooled to room temperature to obtain a brown precipitate, which is then centrifuged, washed, and dried to obtain nano-carbon-coated titanium dioxide particles.

[0048] In certain embodiments of the present invention, in step 1), the titanium dioxide precursor is tetrabutyl titanate.

[0049] In certain embodiments of the present invention, in step 2), the acid is glacial acetic acid; the volume ratio of tetrabutyl titanate to the acid in the titanium dioxide precursor solution is 10-30:1; more preferably, the volume ratio of tetrabutyl titanate to the acid is 10:1.

[0050] In certain embodiments of the present invention, in step 3), the amount of water in the mixed solution is 12%-18% of the volume of tetrabutyl titanate; more preferably, the amount of water is 12% of the volume of tetrabutyl titanate.

[0051] In certain embodiments of the present invention, in step 3), the carbon source is glucose; the amount of the carbon source in the mixed solution is 1%-6% of the amount of tetrabutyl titanate; more preferably, the amount of the carbon source is 3% of the amount of tetrabutyl titanate.

[0052] In certain embodiments of the present invention, in step 4), the stirring speed is 500-1000 rpm.

[0053] In certain embodiments of the present invention, in step 5), the heating reaction temperature is 150-200° C., and the time is 10-20 h; more preferably, the heating reaction temperature is 170° C., and the time is 10 h.

[0054] In certain embodiments of the present invention, in step 6), the centrifugal speed is 10000-15000 rpm, the washing is 3-5 times with anhydrous ethanol, the drying temperature is 55-65° C., and the drying time is 8-12 h.

[0055] As a second aspect of the present invention, the application of nano-carbon coated titanium dioxide particles in preventing and controlling rice fungal diseases comprises the following specific steps:

[0056] 11) Pick a small amount of mycelium from the frozen strain of rice fungal pathogens with a sterile inoculation needle and inoculate it in the middle of the PDA plate, and culture it in a constant temperature incubator at 28±1°C until the mycelium covers the entire plate, and set aside;

[0057] 12) dispersing the nanocarbon-coated titanium dioxide particles in deionized water to prepare a mother solution of a certain concentration;

[0058] 13) adding different volumes of mother solution to PDA culture medium to obtain poisoned PDA culture medium of different mass concentrations;

[0059] 14) Inoculate the bacterial cake (5 mm in diameter) into the poisonous plate and the blank control; place it in a constant temperature incubator at 28±1℃ with the light on for 24-48 hours, and then measure the colony diameter using the cross method.

[0060] In certain embodiments of the present invention, in step 11), the rice fungal disease pathogen is any one or more pathogens of rice sheath blight, rice blast, rice false smut and seedling bakanae.

[0061] In certain embodiments of the present invention, in step 13), the mass concentration of the poisonous PDA culture medium is 10-100 mg / L; more preferably, the mass concentration of the poisonous PDA culture medium is 10 mg / L.

[0062] Embodiment 1:

[0063] A method for preparing nanocarbon-coated titanium dioxide particles comprises the following steps:

[0064] 1) Accurately measure 60 mL of ethanol in a beaker, slowly add 30 mL of tetrabutyl titanate thereto under stirring at room temperature; then add 3 mL of glacial acetic acid to inhibit its hydrolysis to obtain a titanium source precursor solution;

[0065] 2) Accurately measure 30 mL of ethanol and 3.6 mL of deionized water into a measuring cylinder and mix them;

[0066] 3) weighing 3% of the amount of tetrabutyl titanate and dissolving it in a mixed solvent of ethanol and water to obtain a mixed solution;

[0067] 4) Slowly add the carbon source precursor solution to the titanium source precursor solution under vigorous stirring and stir for 30 minutes to form a transparent sol;

[0068] 5) The transparent sol was transferred to a hydrothermal reactor with a polytetrafluoroethylene reaction liner, and the reaction temperature was set to 170°C and the reaction time was 10 h;

[0069] 6) After the reactor is cooled to room temperature, the brown precipitate is centrifuged, washed three times with ethanol, and dried in an oven at 60° C. to obtain nanocarbon-coated titanium dioxide particles.

[0070] An application of nano-carbon coated titanium dioxide particles in preventing and controlling rice fungal diseases comprises the following specific steps:

[0071] Pour 20 mL of PDA medium into a sterile culture dish, wait for it to solidify to make a PDA plate, pick a small amount of mycelium from the frozen strain of Rhizoctonia solani, the pathogen of rice sheath blight, with a sterile inoculation needle and inoculate it in the middle of the PDA plate, and culture it in a constant temperature incubator at 28±1°C until the mycelium covers the entire plate, and set aside; the above operations are all carried out in a clean bench;

[0072] The carbon-coated titanium dioxide nanoparticles prepared above are dispersed in deionized water to prepare a 10000 mg / L mother solution for later use;

[0073] The PDA culture medium was heated and melted in a microwave oven. After the culture medium was cooled to 55±1°C, 60 μL of the mother solution was added and mixed thoroughly to obtain a poisoned PDA culture medium with a nanoparticle mass concentration of 10 mg / L. After solidification, the poisoned PDA plate was prepared. The PDA plate without nanoparticles was used as a blank control. Each treatment was repeated 3 times.

[0074] Take a bacterial cake (5 mm in diameter) from the edge of the pre-cultured pathogen colony with a sterile puncher and inoculate it into the toxic plate and the blank control. After inoculation, place it in a constant temperature incubator at 28±1℃ and turn on the light for 24-48 hours, and then measure the colony diameter using the cross method.

[0075] The transmission electron microscope photo of the prepared nanocarbon-coated titanium dioxide particles is as follows: Figure 1 As shown, a clear carbon coating can be seen; the antifungal results are shown in Figure 2 As shown, nanocarbon-coated titanium dioxide particles had a significant inhibitory effect on the growth of Rhizoctonia solani at a mass concentration of 10 mg / L.

[0076] Example 2

[0077] Example 1 was repeated, except that the applied mass concentration of the nanoparticles was adjusted to 50 mg / L. The antibacterial results were as follows: Figure 2 As shown, nanocarbon-coated titanium dioxide particles have a significant inhibitory effect on the growth of Rhizoctonia solani at a mass concentration of 50 mg / L.

[0078] Example 3

[0079] Example 1 was repeated, except that the applied mass concentration of the nanoparticles was adjusted to 100 mg / L. The antibacterial results are shown in Figure 2 As shown, nanocarbon-coated titanium dioxide particles had a significant inhibitory effect on the growth of Rhizoctonia solani at a mass concentration of 100 mg / L.

[0080] Example 4

[0081] Example 1 was repeated, except that the pathogen was changed to the rice blast pathogen fungus.

[0082] The result is that nanocarbon-coated titanium dioxide particles also have a significant inhibitory effect on the growth of rice blast pathogen fungi.

[0083] Example 5

[0084] Example 1 was repeated, except that the pathogen was changed to the pathogen of rice false smut fungus.

[0085] The results showed that the nanocarbon-coated titanium dioxide particles also had a significant inhibitory effect on the growth of rice false smut pathogen fungi.

[0086] Example 6

[0087] Example 1 was repeated, except that the pathogen was changed to the fungus pathogen of rice bakanae disease.

[0088] The results showed that the nanocarbon-coated titanium dioxide particles also had a significant inhibitory effect on the growth of the pathogenic fungus of rice seedling disease.

[0089] Example 7

[0090] Example 1 was repeated, except that the amount of glucose was adjusted to 1.5% of the amount of tetrabutyl titanate. The antibacterial results are shown in Figure 3 As shown, when the amount of glucose is 1.5% of the amount of tetrabutyl titanate, the nano-carbon coated titanium dioxide particles have a significant inhibitory effect on the growth of Rhizoctonia solani.

[0091] Example 8

[0092] Example 1 was repeated, except that the amount of glucose was adjusted to 4.5% of the amount of tetrabutyl titanate. The antibacterial results are shown in Figure 3 As shown, when the amount of glucose is 4.5% of the amount of tetrabutyl titanate, the nano-carbon coated titanium dioxide particles have a significant inhibitory effect on the growth of Rhizoctonia solani.

[0093] Example 9

[0094] Example 1 was repeated, except that the amount of glucose was adjusted to 6% of the amount of tetrabutyl titanate. The antibacterial results are shown in Figure 3 As shown, when the amount of glucose is 6% of the amount of tetrabutyl titanate, the nano-carbon-coated titanium dioxide particles have a significant inhibitory effect on the growth of Rhizoctonia solani.

[0095] Comparative Example 1

[0096] The difference between this comparative example and Example 1 is that glucose was not added during the preparation process. The other methods were the same as Example 1 to obtain titanium dioxide nanoparticles. The antibacterial results are shown in FIG. Figure 4 shown.

[0097] It can be seen that when the mass concentration of titanium dioxide particles is 10 mg / L, the titanium dioxide particles have no significant inhibitory effect on the growth of Rhizoctonia solani under visible light.

[0098] Comparative Example 2

[0099] The difference between this comparative example and comparative example 1 is that the mass concentration of titanium dioxide particles is adjusted to 50 mg / L. The antibacterial results are as follows: Figure 4 shown.

[0100] It can be seen that when the mass concentration of titanium dioxide particles is 50 mg / L, the titanium dioxide particles have no significant inhibitory effect on the growth of Rhizoctonia solani under visible light.

[0101] Comparative Example 3

[0102] The difference between this comparative example and comparative example 1 is that the mass concentration of titanium dioxide particles is adjusted to 100 mg / L. The antibacterial results are as follows: Figure 4 shown.

[0103] It can be seen that when the mass concentration of titanium dioxide particles is 100 mg / L, the titanium dioxide particles have no significant inhibitory effect on the growth of Rhizoctonia solani under visible light.

[0104] Comparative Example 4

[0105] The difference between this comparative example and Example 1 is that the ratio of tetrabutyl titanate to acid is adjusted to 60:1, and the product is obtained without titanium dioxide complete crystal form. The antibacterial results are as follows Figure 5 shown.

[0106] It can be seen that when the ratio of titanium dioxide precursor to acid is 60:1, there is no significant inhibitory effect on the growth of Rhizoctonia solani.

[0107] Comparative Example 5

[0108] The difference between this comparative example and Example 1 is that the amount of water is adjusted to 8% of the volume of tetrabutyl titanate, and the product is obtained without titanium dioxide complete crystal form. The antibacterial results are as follows: Figure 5 shown.

[0109] It can be seen that when the amount of water is 8% by volume of tetrabutyl titanate, it has no significant inhibitory effect on the growth of Rhizoctonia solani.

[0110] Comparative Example 6

[0111] The difference between this comparative example and Example 1 is that the heating reaction temperature is adjusted to 130°C, and the product is obtained without titanium dioxide complete crystal form. The antibacterial results are as follows Figure 5 shown.

[0112] It can be seen that when the heating reaction temperature is 130℃, there is no significant inhibitory effect on the growth of Rhizoctonia solani.

[0113] Obviously, the above embodiments of the present invention are merely examples for clearly explaining the present invention, and are not intended to limit the implementation methods of the present invention. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is impossible to list all implementation methods here. Any obvious changes or modifications derived from the technical solution of the present invention are still within the scope of protection of the present invention.

Claims

1. A method for preparing nanocarbon-coated titanium dioxide particles, characterized in that: The steps include: 1) dissolving a titanium dioxide precursor in ethanol to form a titanium dioxide precursor solution; 2) adding acid to the titanium dioxide precursor solution; 3) dissolving the carbon source in a mixed solvent of ethanol and water to form a mixed solution; 4) slowly adding the mixed solution into the titanium dioxide precursor solution under stirring, and mixing evenly to form a transparent sol; 5) transferring the transparent sol to a hydrothermal reactor with a polytetrafluoroethylene reaction liner and heating the reaction; 6) After the heating reaction is completed, the reactor is cooled to room temperature to obtain a brown precipitate, which is then centrifuged, washed, and dried to obtain nano-carbon-coated titanium dioxide particles.

2. The method for preparing nanocarbon-coated titanium dioxide particles according to claim 1, characterized in that: In step 1), the titanium dioxide precursor is tetrabutyl titanate.

3. The method for preparing nanocarbon-coated titanium dioxide particles according to claim 2, characterized in that: In step 2), the acid is glacial acetic acid; the volume ratio of tetrabutyl titanate to the acid in the titanium dioxide precursor solution is 10-30:1; more preferably, the volume ratio of tetrabutyl titanate to the acid is 10:

1.

4. The method for preparing nanocarbon-coated titanium dioxide particles according to claim 2, characterized in that: In step 3), the amount of water in the mixed solution is 12%-18% of the volume of tetrabutyl titanate; more preferably, the amount of water is 12% of the volume of tetrabutyl titanate.

5. The method for preparing nanocarbon-coated titanium dioxide particles according to claim 2, characterized in that: In step 3), the carbon source is glucose; the amount of the carbon source in the mixed solution is 1%-6% of the amount of tetrabutyl titanate; more preferably, the amount of the carbon source is 3% of the amount of tetrabutyl titanate.

6. The method for preparing nanocarbon-coated titanium dioxide particles according to claim 1, characterized in that: In step 4), the stirring speed is 500-1000 rpm.

7. The method for preparing nanocarbon-coated titanium dioxide particles according to claim 1, characterized in that: In step 5), the heating reaction temperature is 150-200°C and the time is 10-20h; more preferably, the heating reaction temperature is 170°C and the time is 10h.

8. The method for preparing nanocarbon-coated titanium dioxide particles according to claim 1, characterized in that: In step 6), the centrifugal speed is 10000-15000 rpm, the washing is 3-5 times with anhydrous ethanol, the drying temperature is 55-65° C., and the drying time is 8-12 h.

9. Application of nanocarbon-coated titanium dioxide particles in preventing and controlling fungal diseases of rice, characterized in that: The specific steps include: 11) Pick a small amount of mycelium from the frozen strain of rice fungal pathogens with a sterile inoculation needle and inoculate it in the middle of the PDA plate, and culture it in a constant temperature incubator at 28±1°C until the mycelium covers the entire plate, and set aside; 12) dispersing the nanocarbon-coated titanium dioxide particles in deionized water to prepare a mother solution of a certain concentration; 13) adding different volumes of mother solution to PDA culture medium to obtain poisoned PDA culture medium of different mass concentrations; 14) Inoculate the bacterial cake into the poisonous plate and the blank control; place it in a constant temperature incubator at 28±1℃ with the light on for 24-48 hours, and then measure the colony diameter using the cross method.

10. The use according to claim 9, characterized in that: In step 11), the rice fungal disease pathogen is any one or more pathogens selected from rice sheath blight, rice blast, rice false smut and bakanae disease; Preferably, in step 13), the mass concentration of the poisonous PDA culture medium is 10-100 mg / L; more preferably, the mass concentration of the poisonous PDA culture medium is 10 mg / L.

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