Prompting system and method suitable for precise sample adding of microwell plate

Abstract

The invention discloses a prompting system and method suitable for precise sample adding of a microwell plate, the prompting system comprises a pore plate positioning device, the interior of the pore plate positioning device is used for placing the microwell plate with X transverse rows and Y longitudinal columns of sample adding holes, the pore plate positioning device comprises X + Y pairs of sensors, the height of the sensors is higher than that of the microwell plate, the X pairs of sensors are in one-to-one correspondence with X transverse-row sample adding holes of the microwell plate, the Y pairs of sensors are in one-to-one correspondence with Y longitudinal-row sample adding holes of the microwell plate, each pair of sensors comprises an emitter and a receiver, and the emitter and the receiver are located at the two ends of the same transverse row or the same longitudinal row respectively; the control display panel comprises a processor, a position display unit, a sample adding frequency display unit, a prompt unit, a continuous sample adding button and a sample adding completion button, wherein the processor is electrically connected with the sensors, and the position display unit, the sample adding frequency display unit, the prompt unit, the continuous sample adding button and the sample adding completion button are electrically connected with the processor. The condition that the same sample adding hole needs to add samples for multiple times can be distinguished, the light-transmitting microwell plate and the light-proof microwell plate are both suitable, manual positioning is not needed, and the device can be used for occasions where multiple reagents need to be added.

Description

Technical Field

[0001] The invention relates to the technical field of microplate sample addition, and in particular to a prompt system and method suitable for accurate sample addition on a microplate. Background Art

[0002] 384-well plate is a plate with 384 sample addition holes, mostly composed of 16 horizontal rows and 24 vertical columns, and is mainly used in biotechnology experiments. By adding the test solution on the template into the 384-well plate, it is convenient to carry out multiple groups of experiments, achieve the advantages of rapid sample addition, multiple sample addition groups, etc. However, for the sample addition of the 384-well plate, since the sample addition holes of the 384-well plate are large, the problem of missing or adding more samples is prone to occur when carrying out multiple groups of sample addition, causing the failure of the test results. A variety of auxiliary sample addition technologies have appeared in the prior art, which assist in sample addition and reduce the error rate, but also bring new problems, which are illustrated below.

[0003] In one of the auxiliary sample loading technologies, each sample loading well is equipped with a sample loading sensor and a sample loading indicator light. The sample loading sensor and the sample loading indicator light cooperate with each other to prompt whether the sample has been loaded in the sample loading well. However, it is difficult to distinguish and prompt the situation where the same sample loading well needs to be loaded multiple times, and the sample loading sensor and the sample loading indicator light are installed corresponding to each sample loading well, which makes the processing and manufacturing difficult.

[0004] In another auxiliary sample loading technology, 24 and 16 light-emitting strips are provided in the horizontal and vertical directions of the 384-well plate mounting slot, respectively, and the positions of the sample loading wells are located by the horizontal and vertical light-emitting strips; however, the horizontal and vertical light-emitting strips are provided at the bottom of the 384-well plate mounting slot, which cannot be applied to the opaque 384-well plate.

[0005] In another auxiliary loading technology, the position of the loading wells is located by means of the intersection of the horizontal plate and the vertical plate on the upper part of the plate; however, the movement of the horizontal plate and the vertical plate requires manual operation, which increases the workload greatly, and the loading wells of the 384-well plate are relatively densely packed, and the accuracy of the positioning device and the shielding effect both increase the difficulty of the loading work.

[0006] In another auxiliary sample loading technology, the indicator light is turned on by pressing a button on the marking cover to indicate the sample loading wells that have been loaded. After the loading of a column of sample loading wells is completed, all the indicator lights are turned off by the main switch, and the sample loading wells in the next column are moved horizontally to load the sample. However, this solution can only load samples in sequence by column and cannot be used in applications where multiple reagents need to be added. Summary of the invention

[0007] To this end, the present invention provides a prompt system and method suitable for accurate sample addition in a microplate to solve one or more of the above-mentioned technical problems.

[0008] In order to achieve the above object, the present invention provides the following technical solutions:

[0009] The first aspect of the present invention provides a prompt system suitable for accurate sample addition in a microplate, comprising:

[0010] A well plate positioning device, the inside of which is used to place a microplate with X horizontal rows and Y vertical columns of sample wells, the well plate positioning device includes X+Y pairs of sensors, the height of the sensors is higher than the microplate, the X pairs of sensors correspond to the X horizontal rows of sample wells of the microplate one by one, the Y pairs of sensors correspond to the Y vertical columns of sample wells of the microplate one by one, each pair of sensors includes a transmitter and a receiver, and the transmitter and the receiver are respectively located at the two ends of the same horizontal row or the same vertical column;

[0011] A control display panel, comprising a processor electrically connected to each of the sensors, and a position display unit, a sample loading times display unit, a prompt unit, a continue loading button, and a sample loading completion button electrically connected to the processor;

[0012] Wherein, the processor determines the position of the current sample loading hole based on the horizontal row and vertical column where the two pairs of sensors sensing the sample loading signal are located and displays it on the position display unit; each time the current sample loading hole is loaded, the processor adds one to the total number of sample loadings of the current sample loading hole and displays it on the sample loading number display unit; after the current sample loading hole is completed, if the total number of sample loadings is equal to the sample loading number threshold, the sample loading completion button is clicked, and the processor records that the total sample loading of the current sample loading hole has been completed; if the total number of sample loadings is less than the sample loading number threshold, the continue sample loading button is clicked, and the processor records that the total sample loading of the current sample loading hole has not been completed; before the current sample loading hole is loaded, the processor determines whether the current sample loading hole has completed the total sample loading, and if it has been completed, the prompt unit is controlled to prompt.

[0013] Furthermore, the well plate positioning device also includes an upper cover plate, which is spaced apart above the microplate, and is provided with auxiliary positioning holes corresponding to the sample loading holes one by one. The edge of each auxiliary positioning hole extends downward to form a conical limiting cylinder that is larger at the top and smaller at the bottom to limit the movable space of the pipette in the horizontal and vertical directions. The lower port of the limiting cylinder is higher than the sensing area of ​​the sensor, and the lower port of the limiting cylinder is used for the gun tip of the pipette to extend downward and extend into the sensing area.

[0014] Furthermore, the orifice plate positioning device also includes a base, the upper cover plate is fixed to the base via a support column, and the sensor is fixed to the base via a bracket.

[0015] Furthermore, reinforcement ribs are arranged between adjacent support columns.

[0016] Furthermore, the sensor includes a laser transmitter and a laser receiver.

[0017] Furthermore, the prompt unit includes a light prompter and a sound prompter.

[0018] Furthermore, the control display panel also includes two groups of prompt words, and the two groups of prompt words are respectively located on one side of the continue adding sample button and the sample adding completion button, for prompting "continue adding" and "completed".

[0019] Furthermore, the microplate with X horizontal rows and Y vertical columns of sample addition wells is a 96-well plate with 8 horizontal rows and 12 vertical columns or a 384-well plate with 16 horizontal rows and 24 vertical columns.

[0020] The second aspect of the present invention provides a prompting method suitable for accurate sample addition on a microplate, which uses the prompting system provided by the first aspect of the present invention, and comprises the following steps:

[0021] Step S1, the pipette is aimed at the sample wells in the xth horizontal row and yth vertical column to add samples;

[0022] Step S2, the sensors in the xth row and the yth column sense the pipette gun and send x and y signals to the processor;

[0023] Step S3, the processor determines the position of the current sample loading hole corresponding to the pipette gun based on the x and y signals, and sends the position signal to the position display unit and the total number of sample loadings to the sample loading number display unit;

[0024] Step S4, the position display unit displays the position of the current sample well, and the sample number display unit displays the total number of sample additions of the current sample well; in the initial state, the total number of sample additions of each sample well is 0;

[0025] Step S5, the processor determines whether the total sample addition of the current sample well has been completed. If not, step S6 is executed; if completed, step S7 is executed. In the initial state, each sample well is in the state of not completing the total sample addition;

[0026] Step S6, adding a sample to the current sample well, and the processor adds one to the total number of sample additions of the current sample well; after adding one, if the total number of sample additions is equal to the sample addition number threshold, the sample addition completion button is clicked, and the processor records that the total sample additions of the current sample well have been completed; if the total number of sample additions is less than the sample addition number threshold, the sample addition continuation button is clicked, and the processor records that the total sample additions of the current sample well have not been completed;

[0027] Step S7, the prompting unit prompts not to add sample to the current sample well;

[0028] Step S8, repeat the above steps S1-S7.

[0029] The present invention has the following advantages:

[0030] 1. Compared with the method of "configuring one sample adding sensor and one sample adding indicator light for each sample adding well", the present invention records whether the total sample adding has been completed in the current sample adding well and gives a prompt through a prompt unit, which can distinguish the situation where the same sample adding well needs multiple sample adding, avoids over-addition or missed addition, and uses fewer sensors (X+Y in the present invention, X×Y in the prior art) and prompt units (one light prompter and one sound prompter in the present invention, X×Y indicator lights in the prior art), and the difficulty of processing and manufacturing is relatively low;

[0031] 2. Compared with the method of "locating the sample well position by luminous bands in both horizontal and vertical directions", the present invention forms a sensing area on the upper side of the microplate (the height of the sensor is higher than the microplate, the X pairs of sensors correspond to the X horizontal rows of sample wells of the microplate, the Y pairs of sensors correspond to the Y vertical columns of sample wells of the microplate, each pair of sensors includes a transmitter and a receiver, and the transmitter and the receiver are respectively located at the two ends of the same horizontal row or the same vertical column), which is not affected by whether the microplate is light-transmissive, and has a wider range of applications;

[0032] 3. Compared with the method of "locating the position of the sample loading hole by means of the intersection of the horizontal plate and the vertical plate placed on the upper part of the plate", the present invention can quickly determine the position of the current sample loading hole by obtaining the information of the horizontal row and vertical column where the two pairs of sensors that sense the sample loading signal are located. There is no need to manually move the horizontal plate and the vertical plate. The positioning is performed by the sensor, which also avoids the problem of increased difficulty in the sample loading work due to the shielding effect during the positioning process of the plate structure;

[0033] 4. Compared with the method of "lighting the indicator light to indicate the sample wells that have been loaded with samples by pressing the button on the marking cover, turning off all the indicator lights by the main switch after the loading of a column of sample wells is completed, and moving horizontally to the next column of sample wells to load samples", the present invention can load samples in disorder, is not limited to the form of "loading samples row by row" or "loading samples column by column", and is suitable for occasions where multiple reagents need to be added.

[0034] The above summary is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the present application will be readily apparent by reference to the accompanying drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0036] The structures, proportions, sizes, etc. illustrated in this specification are only used to match the contents disclosed in the specification so as to facilitate understanding and reading by persons familiar with the technology. They are not used to limit the conditions under which the present invention can be implemented, and therefore have no substantial technical significance. Any structural modification, change in proportion or adjustment of size shall still fall within the scope of the technical contents disclosed in the present invention without affecting the effects and purposes that can be achieved by the present invention.

[0037] Figure 1 A schematic diagram of the structure of a prompt system suitable for accurate sample addition on a microplate provided by an embodiment of the present invention;

[0038] Figure 2 A schematic side cross-sectional view of a well plate positioning device for a prompt system for accurate sample addition to a microplate provided by an embodiment of the present invention;

[0039] Figure 3 A horizontal cross-sectional schematic diagram (viewed from top to bottom) of a well plate positioning device of a prompt system for accurate sample addition to a microwell plate provided in an embodiment of the present invention;

[0040] Figure 4 A schematic diagram of a prompt method for accurate sample addition in a microplate provided in an embodiment of the present invention.

[0041] In the figure: 1. well plate positioning device; 2. signal transmission cable; 3. control display panel; 4. 384-well plate; 101. base; 102. support column; 103. upper cover plate; 104. laser transmitter; 105. laser receiver; 106. laser line; 107. auxiliary sample loading hole; 108. limit cylinder; 109. bracket; 110. long reinforcement rib; 111. short reinforcement rib; 301. position display unit; 302. sample loading number display unit; 303. prompt word; 304. light prompter; 305. sound prompter; 306. sample loading completion button; 307. continue sample loading button; 401. sample loading hole. DETAILED DESCRIPTION

[0042] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the present application. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0043] like Figure 1-3 As shown, this embodiment provides a prompt system suitable for accurate sample addition in a microplate, which includes a microplate positioning device 1 and a control display panel 3 , and the microplate positioning device 1 and the control display panel 3 are connected via a signal transmission cable 2 .

[0044] The interior of the well plate positioning device 1 is used to place a microplate with X horizontal rows and Y vertical columns of sample wells 401. The microplate is a 96-well plate with 8 horizontal rows and 12 vertical columns or a 384-well plate 4 with 16 horizontal rows and 24 vertical columns. In this embodiment, the 384-well plate 4 with 16 horizontal rows and 24 vertical columns is taken as an example. The 16 horizontal rows are marked as "A", "B", "C", "D", ..., "M", "N", "O", "P", and the 24 vertical columns are marked as "1", "2", "3", "4", ..., "22", "23", "24", respectively.

[0045] The orifice plate positioning device 1 includes a base 101, a plurality of support columns 102, a plurality of brackets 109, 40 pairs of sensors and an upper cover plate 103. The base 101 serves as the foundation of the orifice plate positioning device 1 and is used to install the support columns 102, the brackets 109, the sensors, the upper cover plate 103, etc. It is also used to install the 384-hole plate 4. Generally, the 384-hole plate 4 is installed in the center of the bottom plate. Since the 384-hole plate 4 is a rectangular plate, the bottom plate is preferably a rectangular plate, but the length and width of the bottom plate are respectively greater than the length and width of the 384-hole plate 4. A vertical support column 102 is arranged at each of the four corners of the rectangular bottom plate, and a long reinforcement rib 110 is arranged between two adjacent support columns 102 in the length direction of the bottom plate, and a short reinforcement rib 111 is arranged between two adjacent support columns 102 in the length direction of the bottom plate, so as to improve the stability of the four support columns 102. The bracket 109 surrounds the bottom plate and surrounds the area where the 384-well plate 4 is installed. The sensor is installed on the bracket 109. The bracket 109 makes the height of the sensor higher than the upper surface of the 384-well plate 4. In this way, the sensing area of ​​the sensor can be located above the sample addition hole 401, wherein 16 pairs of sensors correspond to the 16 horizontal rows of the microplate, and 24 pairs of sensors correspond to the 24 vertical columns of the microplate. The sensor in this embodiment takes a laser sensor as an example, and other forms of sensors can also be used. Each pair of laser sensors includes a laser transmitter 104 and a laser receiver 105, respectively. The transmitter and the receiver are respectively located at the two ends of the same horizontal row or the same vertical column, and all the transmitters and receivers are in the same plane. In this way, the lasers crisscross to form 384 intersections, which correspond to the 384 sample addition holes 401 one by one, and the criss-crossed laser lines 106 form the sensing area mentioned above. The upper cover plate 103 is installed on the top of the support column 102, and its area can cover the 384-well plate 4 below. There is a gap between the upper cover plate 103 and the 384-well plate 4; 384 auxiliary positioning holes are arranged on the upper cover plate 103, and the 384 auxiliary positioning holes are arranged one by one corresponding to the 384 sample addition holes 401 of the 384-well plate 4. A conical limiting cylinder 108 with a larger upper part and a smaller lower part extends downward from the edge of each auxiliary positioning hole to limit the activity of the pipette gun in the horizontal and vertical directions. The lower end of the limiting cylinder 108 is higher than the sensing area of ​​the sensor to avoid affecting the laser positioning; the lower end of the limiting cylinder 108 is used for the tip of the pipette to extend downward. When the tip is extended downward, it will extend into the sensing area, and at this time, it will block the horizontal and vertical laser lines 106 on the current sample loading hole 401; wherein, the spacing between the upper cover plate 103 and the 384-well plate 4 is approximately equal to the length of the pipette tip, and the height of the limiting cylinder 108 accounts for approximately 2 / 3 of the length of the pipette tip.

[0046] The control display panel 3 includes a processor electrically connected to each sensor and a position display unit 301 electrically connected to the processor, a sampling number display unit 302, a prompt unit, a continue sampling button 307 and a sampling completion button 306; wherein the prompt unit includes a light prompter 304 and a sound prompter 305, a "continue adding" prompt word 303 is set below the continue sampling button 307, and a "completed" prompt word 303 is set below the sampling completion button 306.

[0047] The processor determines the position of the current sample loading hole 401 based on the horizontal row and vertical column where the two pairs of sensors sensing the sample loading signal are located, and displays it on the position display unit 301; each time the current sample loading hole 401 is loaded, the processor adds one to the total number of sample loadings of the current sample loading hole 401 and displays it on the sample loading number display unit 302; after the current sample loading hole 401 is completed, if the total number of sample loadings is equal to the sample loading number threshold, the sample loading completion button 306 is clicked, and the processor records that the current sample loading hole 401 has completed the total sample loading; if the total number of sample loadings is less than the sample loading number threshold, the continue sample loading button 307 is clicked, and the processor records that the current sample loading hole 401 has not completed the total sample loading; before the current sample loading hole 401 is loaded, the processor determines whether the current sample loading hole 401 has completed the total sample loading, and if it has been completed, the prompt unit is controlled to prompt.

[0048] Based on the above prompt system, this embodiment also provides a prompt method suitable for accurate sample addition on a microplate, such as Figure 4 As shown, the prompt method takes the addition of samples to the sample well 401 in the 4th row (i.e., row D) and the 10th column in the 384-well plate 4 as an example, and includes the following steps:

[0049] Step S1, aiming the pipette at the sample well 401 in the 4th horizontal row and the 10th vertical column to add samples.

[0050] Step S2, the sensors in the 4th row and the 10th column sense the pipette gun and send 4 (D) row and 10 column signals to the processor. Specifically, after the pipette gun is extended downward, the laser line 106 emitted by the laser emitter 104 in the 4th row and the 10th column is blocked by the pipette gun tip, and the laser receiver 105 in the 4th row and the 10th column cannot receive the laser line 106, and sends a "0" signal to the controller (when the laser receiver 105 can receive the laser line 106, it sends a "1" signal to the controller, and the "1" signal here is not a "column" signal).

[0051] In step S3 , the processor determines the position of the current sample loading hole 401 corresponding to the pipette based on the 4 (D) row and 10 column signals, that is, the current sample loading hole 401 is D10 , and sends the position signal to the position display unit 301 and the total number of loading times to the loading times display unit 302 .

[0052] Step S4, the position display unit 301 displays the position of the current sample well 401, and the sample number display unit 302 displays the total number of sample additions of the current sample well 401; in the initial state, the total number of sample additions of each sample well 401 is 0. Specifically, the screen of the position display unit 301 corresponds to 16 horizontal rows and 24 vertical columns of squares, and the numbers "1"-"24" are marked from left to right on the top of the squares, and the letters "A"-"P" are marked from top to bottom on the left side of the squares. At this time, the small squares corresponding to the letter "D" and the number "10" are highlighted (for example, different colors can be displayed); the sample number display unit 302 displays the total number of sample additions of the sample well 401 corresponding to "D10". For the first sample addition, the total number of sample additions at this time is "0", and for the sample well 401 that has been loaded with samples for multiple times, the total number of sample additions at this time is the actual number of sample additions.

[0053] In step S5, the processor determines whether the total loading of the current sample loading hole 401 has been completed. If not, step S6 is executed; if completed, step S7 is executed; in the initial state, each sample loading hole 401 is in the state of not completing the total loading. This prompt system can meet the needs of different reagents loaded in different sample loading holes 401 (different loading times), so each sample loading hole 401 is set with its own loading times threshold. For example, the loading times threshold of some sample loading holes 401 is "1", which means that the sample loading hole 401 only needs to be loaded once, and the loading times threshold of some sample loading holes 401 is "5", which means that the sample loading hole 401 needs to be loaded 5 times; when the total loading times is equal to the loading times threshold, it means that the current sample loading hole 401 has completed the total loading, and by clicking the loading completion button 306, the processor records the position information of the sample loading hole 401.

[0054] Step S6, add sample to the current sample well 401, and the processor adds one to the total number of sample additions of the current sample well 401; after adding one, if the total number of sample additions is equal to the sample addition number threshold, the sample addition completion button 306 is clicked, and the processor records that the total sample addition of the current sample well 401 has been completed; if the total number of sample additions is less than the sample addition number threshold, the continue sample addition button 307 is clicked, and the processor records that the total sample addition of the current sample well 401 has not been completed.

[0055] Step S7, the prompting unit gives a prompt and does not add a sample to the current sample adding hole 401. Specifically, the light prompter 304 intermittently emits a red light prompt, and the sound prompter 305 continuously emits an "error" prompt sound.

[0056] Step S8, repeat the above steps S1-S7.

[0057] The prompting system and method provided in this embodiment have at least the following advantages:

[0058] 1. Compared with the method of "configuring one sample adding sensor and one sample adding indicator light for each sample adding well", the present invention records whether the total sample adding has been completed in the current sample adding well and gives a prompt through a prompt unit, which can distinguish the situation where the same sample adding well needs multiple sample adding, avoids over-addition or missed addition, and uses fewer sensors (40 in the present invention, 384 in the prior art) and prompt units (one light prompter and one sound prompter in the present invention, 384 indicator lights in the prior art), and is less difficult to manufacture;

[0059] 2. Compared with the method of "locating the sample well position by luminous bands in both horizontal and vertical directions", the present invention forms a sensing area on the upper side of the microplate (the height of the sensor is higher than the microplate, 16 pairs of sensors correspond to the 16 horizontal rows of sample wells of the microplate, 24 pairs of sensors correspond to the 24 vertical columns of sample wells of the microplate, each pair of sensors includes a transmitter and a receiver, and the transmitter and the receiver are respectively located at the two ends of the same horizontal row or the same vertical column), which is not affected by whether the microplate is light-transmissive, and has a wider range of applications;

[0060] 3. Compared with the method of "locating the position of the sample loading hole by means of the intersection of the horizontal plate and the vertical plate placed on the upper part of the plate", the present invention can quickly determine the position of the current sample loading hole by obtaining the information of the horizontal row and vertical column where the two pairs of sensors that sense the sample loading signal are located. There is no need to manually move the horizontal plate and the vertical plate. The positioning is performed by the sensor, which also avoids the problem of increased difficulty in the sample loading work due to the shielding effect during the positioning process of the plate structure;

[0061] 4. Compared with the method of "lighting the indicator light to indicate the sample wells that have been loaded with samples by pressing the button on the marking cover, turning off all the indicator lights by the main switch after the loading of a column of sample wells is completed, and moving horizontally to the next column of sample wells to load samples", the present invention is not limited to the form of "loading samples row by row" or "loading samples column by column", and can load samples in disorder without error, which is suitable for occasions where multiple reagents need to be added.

[0062] In the description of this specification, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0063] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0064] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or a communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0065] In the present application, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0066] The disclosure above provides many different embodiments or examples to realize the different structures of the present application. In order to simplify the disclosure of the present application, the parts and settings of specific examples are described above. Of course, they are only examples, and the purpose is not to limit the present application. In addition, the present application can repeat reference numerals and / or reference letters in different examples, and this repetition is for the purpose of simplification and clarity, and does not indicate the relationship between the various embodiments and / or settings discussed in itself.

[0067] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can easily think of various changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. A prompt system suitable for accurate sample addition in a microplate, characterized in that: include: A well plate positioning device (1), the interior of which is used to place a microplate having X horizontal rows and Y vertical columns of sample wells (401), the well plate positioning device (1) comprising X+Y pairs of sensors, the height of the sensors being higher than the microplate, the X pairs of sensors corresponding one-to-one to the X horizontal rows of sample wells (401) of the microplate, the Y pairs of sensors corresponding one-to-one to the Y vertical columns of sample wells (401) of the microplate, each pair of sensors comprising a transmitter and a receiver, the transmitter and the receiver being located at two ends of the same horizontal row or the same vertical column; A control display panel (3) comprising a processor electrically connected to each of the sensors, and a position display unit (301), a sample loading times display unit (302), a prompt unit, a continue loading button (307), and a sample loading completion button (306) electrically connected to the processor; The processor determines the position of the current sample loading hole (401) based on the horizontal row and vertical column where the two pairs of sensors sensing the sample loading signal are located, and displays it on the position display unit (301); each time the current sample loading hole (401) is loaded with a sample, the processor adds one to the total number of sample loadings of the current sample loading hole (401) and displays it on the sample loading number display unit (302); after the current sample loading hole (401) is loaded with a sample, if the total number of sample loadings is equal to the sample loading number threshold, the sample loading completion button (306) is pressed, and the processor records that the current sample loading hole (401) has completed the total sample loading; if the total number of sample loadings is less than the sample loading number threshold, the continue sample loading button (307) is pressed, and the processor records that the current sample loading hole (401) has not completed the total sample loading; before the current sample loading hole (401) is loaded with a sample, the processor determines whether the current sample loading hole (401) has completed the total sample loading, and if it has been completed, the prompt unit is controlled to prompt.

2. The prompt system for accurate sample addition on a microplate according to claim 1, characterized in that: The well plate positioning device (1) also includes an upper cover plate (103), which is arranged at intervals above the microplate, and is provided with auxiliary positioning holes corresponding to the sample loading holes (401) one by one on the upper cover plate (103). The edge of each auxiliary positioning hole extends downward to form a conical limiting cylinder (108) that is larger at the top and smaller at the bottom to limit the movable space of the pipette in the horizontal and vertical directions. The lower end of the limiting cylinder (108) is higher than the sensing area of ​​the sensor, and the lower end of the limiting cylinder (108) is used for the tip of the pipette to extend downward and extend into the sensing area.

3. The prompt system for accurate sample addition on a microplate according to claim 2, characterized in that: The orifice plate positioning device (1) further comprises a base (101), the upper cover plate (103) is fixed to the base (101) via a support column (102), and the sensor is fixed to the base (101) via a bracket (109).

4. The prompt system for accurate sample addition on a microplate according to claim 3, characterized in that: Reinforcement ribs are arranged between adjacent support columns (102).

5. The prompt system for accurate sample addition in a microplate according to claim 1, characterized in that: The sensor comprises a laser transmitter (104) and a laser receiver (105).

6. The prompt system for accurate sample addition in a microplate according to claim 1, characterized in that: The prompting unit comprises a light prompter (304) and a sound prompter (305).

7. The prompt system for accurate sample addition in a microplate according to claim 1, characterized in that: The control display panel (3) further comprises two groups of prompt words (303), the two groups of prompt words (303) being respectively located on one side of the continue adding sample button (307) and the sample adding completion button (306), for prompting "continue adding" and "complete".

8. The prompt system for accurate sample addition on a microplate according to any one of claims 1 to 7, characterized in that: The microwell plate with X-row and Y-column sample addition wells (401) is a 96-well plate with 8-row and 12-column wells or a 384-well plate with 16-row and 24-column wells (4).

9. A prompt method suitable for accurate sample addition in a microplate, characterized in that: Using the prompt system according to any one of claims 1 to 8 comprises the following steps: Step S1, aiming the pipette at the sample well (401) in the xth horizontal row and yth vertical column to add sample; Step S2, the sensors in the xth row and the yth column sense the pipette gun and send x and y signals to the processor; Step S3, the processor determines the position of the current sample loading hole (401) corresponding to the pipette based on the x and y signals, and sends the position signal to the position display unit (301), and sends the total number of sample loadings to the sample loading number display unit (302); Step S4, the position display unit (301) displays the position of the current sample loading hole (401), and the sample loading number display unit (302) displays the total number of sample loadings of the current sample loading hole (401); in the initial state, the total number of sample loadings of each sample loading hole (401) is 0; Step S5, the processor determines whether the total sample loading of the current sample loading hole (401) has been completed, if not, step S6 is executed, if completed, step S7 is executed; In the initial state, each sample loading hole (401) is in an uncompleted total loading state; Step S6, adding a sample to the current sample well (401), and the processor adds one to the total number of sample additions of the current sample well (401); after adding one, if the total number of sample additions is equal to the sample addition number threshold, the sample addition completion button (306) is clicked, and the processor records that the total sample addition of the current sample well (401) has been completed; if the total number of sample additions is less than the sample addition number threshold, the sample addition continuation button (307) is clicked, and the processor records that the total sample addition of the current sample well (401) has not been completed; Step S7, the prompting unit prompts not to add sample to the current sample adding hole (401); Step S8, repeat the above steps S1-S7.

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