Skin care product containing gastrodia elata component and preparation method thereof
By using Gastrodia elata active peptides and other natural extracts in skin care products, the adverse reactions of existing skin care whitening ingredients on the skin are solved, and safer and more effective whitening, anti-inflammatory and moisturizing effects are achieved.
Patent Information
- Application Number
- CN202411860886.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-17
- Publication Date
- 2025-05-02
AI Technical Summary
The whitening ingredients in existing skin care products often have adverse reactions to the skin, leading to inflammation and dry itchy problems.
Using skin care products containing Gastrodia elata ingredients, a skin care product with anti-inflammatory, whitening and moisturizing effects is prepared through a combination of Gastrodia elata active peptide, green tea extract, licorice extract and aloe extract.
Through the antibacterial and anti-inflammatory effects of Gastrodia elata polypeptide, this skin care product inhibits tyrosinase to reduce melanin production, significantly improves the whitening effect of the skin, while enhancing anti-inflammatory and moisturizing properties, and reducing adverse reactions.
Smart Images
Figure CN119908996A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of skin care product manufacturing, and specifically relates to a skin care product containing gastrodia elata ingredients and a preparation method thereof. Background Art
[0002] Skin care products, that is, products that protect the skin. With the continuous progress of social economy and the enrichment of material life, skin care products are no longer something that only the rich can afford. Nowadays, skin care products have entered the homes of ordinary people, and they have played a great role in improving people's spirit and image. First of all, skin care products have the function of nourishing the skin and can enhance the elasticity and vitality of the skin. Regular use can make people young and beautiful. Secondly, skin care products enhance people's self-confidence, improve people's good image, and promote social civilization. There are many types of skin care products with different characteristics. When using them, you must choose them according to your actual situation. Don't follow the crowd. Because people's skin is different, there are oily skin, dry skin, neutral skin, mixed skin and allergic skin. Therefore, the manufacturing ingredients of each skin care product are different according to the nature of different skin.
[0003] Most of the whitening ingredients contained in existing skin care products will have adverse reactions on the skin, causing damage to the skin. The adverse reactions include stimulating inflammation, dry and itchy skin, etc. Summary of the invention
[0004] In view of the deficiencies in the prior art, the present invention provides a skin care product containing Gastrodia elata ingredients and a preparation method thereof to solve the above technical problems.
[0005] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a skin care product containing Gastrodia elata ingredients, characterized in that the skin care product is prepared from the following materials: 20-60 parts of Gastrodia elata active peptides, 30-80 parts of basic skin care products, 10-30 parts of emulsifiers, 10-20 parts of essential oils, 20-40 parts of green tea extracts, 10-30 parts of licorice extracts, and 10-20 parts of aloe extracts.
[0006] Preferably, the skin care product is prepared from the following materials: 40 parts of Gastrodia elata active peptide, 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 30 parts of green tea extract, 20 parts of licorice extract and 10 parts of aloe extract.
[0007] Preferably, the mass ratio of Gastrodia elata active peptide, green tea extract and licorice extract is 4:3:2, and the Gastrodia elata peptide active ingredients include Gastrodia elata polypeptide and gastrodin.
[0008] A skin care product containing gastrodia elata ingredient and a preparation method thereof, the preparation method comprising: S1: prepare 40 parts of Gastrodia elata active peptide, 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 30 parts of green tea extract, 20 parts of licorice extract and 10 parts of aloe extract; S2: Put the basic skin care product and emulsifier into a mixing container, heat and stir until the emulsifier is completely dissolved and a uniform emulsion is formed; S3: Add green tea extract and licorice extract to the mixture of emulsifier and basic skin care products, and stir well; S4: When the temperature of the mixture drops to about 40°C, add Gastrodia elata active peptide and aloe vera extract, and continue stirring until completely mixed; S5: When the mixture is cooled to below 40°C, adding the essential oil; S6: Use a pH meter to detect the pH value of the product, and use an appropriate pH regulator to adjust the pH value to between 4.5 and 5.5; S7: Continue stirring the mixture until the desired texture and consistency are achieved, conduct quality tests on the finished product, including stability testing, skin compatibility testing, and effectiveness testing, fill the prepared skin care product into containers, and seal them.
[0009] Preferably, S2 specifically includes: Prepare stainless steel containers and sterilize them in advance; Weigh 50 parts of basic skin care products and 20 parts of emulsifiers, and pour them into the prepared stainless steel containers respectively; Place the mixing container on a heating device and set the heating temperature to 70-75°C. During the heating process, use a thermometer to monitor the temperature of the mixture in real time; Stir the mixture using a magnetic stirrer at medium speed; The stirring time is 15-30 minutes; Check the state of the mixture regularly to ensure that the emulsifier has been completely dissolved and there are no particles or stratification. If the emulsifier is not completely dissolved and there are lumps, continue heating and stirring until the emulsion reaches a uniform state; When the emulsifier is completely dissolved and the emulsion is uniform, remove the magnetic stirrer from the heating device; Allow the mixture to cool naturally to 40°C-45°C; When the mixture has cooled to the desired temperature of 40°C-45°C, check the viscosity and homogeneity of the emulsion.
[0010] S3 specifically includes: Green tea extract and licorice extract were added in stages; Gradually increase the stirring speed; Use vacuum degassing equipment to remove air bubbles from the mixture; Check the homogeneity and stability of the emulsion.
[0011] Preferably, S4 specifically includes: Use temperature control equipment to monitor the temperature of the mixture and ensure that it drops to 40°C. If the temperature is still above 40°C, wait for it to cool down naturally; Slowly add Gastrodia elata active peptide, and then slowly add aloe vera extract after adding Gastrodia elata active peptide; While adding the extract, start the magnetic stirrer to stir at a low speed, and gradually increase the stirring speed to ensure that the extract is fully mixed into the basic skin care product; Observe the color and texture of the mixture and take a small amount of the mixture for further testing to check the uniform distribution of the extract and the stability of the product; Continue stirring until the mixture reaches a completely homogeneous state, stirring time is 15-30 minutes, and check the final state of the mixture.
[0012] Preferably, S5 specifically includes: Accurately weigh the required amount of essential oil according to the proportion of the recipe; Monitor the temperature of the mixture and let it cool to 25°C-30°C; Use a dropper to add the measured essential oils to the mixture; Start the magnetic stirrer to stir at a low speed and gradually increase the stirring speed; Take a small amount of the mixture to check to ensure that the essential oils are evenly distributed and there is no precipitation or stratification; Fill the final mixture into containers.
[0013] Preferably, S6 specifically includes: Use standard pH buffer to calibrate the pH meter; Take a product sample, immerse the pH meter probe into the sample, wait for a stable reading, and record the current pH value; Add pH adjuster and check the pH value of the product again using a pH meter until the pH value is between 4.5-5.5.
[0014] Preferably, the Gastrodia elata extraction step specifically comprises: Grinding the Gastrodia elata medicinal material into Gastrodia elata powder; Degreasing with 95% ethanol, at a solid-liquid ratio of 1:10 (g / ml), twice, 1 hour each time, recovering the ethanol, collecting the fat-soluble components and defatted Gastrodia elata powder; The defatted Gastrodia elata powder was extracted with 10% ethanol at 80°C, 20 times the amount, twice, 2 hours each time; The extract was filtered, concentrated to 1:2, refrigerated, and filtered; 1% papain and 0.8% trypsin were added to the filtrate in a volume ratio; Add alcohol to 80% and refrigerate overnight; The supernatant was passed through an ultrafiltration membrane and centrifuged at 90,000 rpm for 20 min to obtain the Gastrodia elata polypeptide; The precipitate is collected, vacuum dried, and crushed into fine powder to obtain Gastrodia elata polysaccharide.
[0015] Compared with the prior art, the present invention has the following beneficial effects: The present application can effectively carry out anti-inflammatory and acne removal by adding Gastrodia elata peptides. The Gastrodia elata polypeptide composite extract has a high antibacterial rate against Staphylococcus aureus and Propionibacterium acnes. The addition of Gastrodia elata polysaccharide can effectively inhibit tyrosinase, which is the rate-limiting enzyme in the melanin synthesis pathway. It mainly affects the production of melanin by affecting the conversion of tyrosine into dopa and the oxidation of dopa into dopaquinone. Therefore, inhibiting the activity of tyrosinase can reduce the production of melanin to a certain extent, and play a role in whitening and brightening the skin, thereby making the whitening and spot-removing effect of the skin care product better. The addition of green tea extract can make the effect of Gastrodia elata peptide volatilize more completely. The addition of licorice extract can make the skin care product more anti-inflammatory. The addition of aloe vera extract can make the skin care product more moisturizing. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 It is a preparation process framework diagram of the present invention; Figure 2 This is a test picture of the whitening effect of the active ingredient of Gastrodia elata peptide; Figure 3 This is a graph showing the free radical scavenging rate of the active ingredient of Gastrodia elata peptide; Figure 4 This is a test chart of the antibacterial and anti-inflammatory effects of the active ingredient of Gastrodia elata peptide. DETAILED DESCRIPTION
[0017] In order to better understand the present application, a more detailed description will be made of various aspects of the present application with reference to the accompanying drawings. It should be understood that these detailed descriptions are only descriptions of exemplary embodiments of the present application, and are not intended to limit the scope of the present application in any way. Throughout the specification, the same reference numerals refer to the same elements. The expression "and / or" includes any and all combinations of one or more of the associated listed items.
[0018] In the accompanying drawings, the size, dimensions and shapes of the elements have been slightly adjusted for ease of illustration. The drawings are for illustration only and are not strictly drawn to scale. As used herein, the terms "substantially", "approximately" and similar terms are used as terms of approximation, not as terms of degree, and are intended to illustrate the inherent deviations in measurements or calculations that would be recognized by a person of ordinary skill in the art. In addition, in this application, the order in which the processing of each step is described does not necessarily represent the order in which these processes occur in actual operation, unless otherwise specified or can be derived from the context.
[0019] It should also be understood that expressions such as "include", "including", "have", "contain" and / or "comprising" are open rather than closed expressions in this specification, which indicate the presence of the stated features, elements and / or components, but do not exclude the presence of one or more other features, elements, components and / or combinations thereof. In addition, when expressions such as "at least one of..." appear after a list of listed features, they modify the entire list of features rather than just the individual elements in the list. In addition, when describing embodiments of the present application, "may" is used to mean "one or more embodiments of the present application". And, the term "exemplary" is intended to refer to an example or illustration.
[0020] Unless otherwise defined, all words (including engineering terms and technical terms) used in this article have the same meaning as commonly understood by ordinary technical citizens in the field to which this application belongs. It should also be understood that, unless otherwise clearly stated in this application, words defined in commonly used dictionaries should be interpreted as having the same meaning as their meaning in the context of the relevant technology, and should not be interpreted in an idealized or overly formal sense.
[0021] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments in the present application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.
[0022] The present invention provides a technical solution: a skin care product containing gastrodia elata ingredients, characterized in that the skin care product is prepared from the following materials: 20-60 parts of gastrodia elata active peptides, 30-80 parts of basic skin care products, 10-30 parts of emulsifiers, 10-20 parts of essential oils, 20-40 parts of green tea extracts, 10-30 parts of licorice extracts, and 10-20 parts of aloe extracts.
[0023] Furthermore, the skin care product is specifically prepared from the following materials: 40 parts of Gastrodia elata active peptide, 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 30 parts of green tea extract, 20 parts of licorice extract and 10 parts of aloe extract.
[0024] Furthermore, the mass ratio of Gastrodia elata active peptide, green tea extract and licorice extract is 4:3:2, and the Gastrodia elata peptide active ingredient includes Gastrodia elata polypeptide and gastrodin.
[0025] A skin care product containing gastrodia elata ingredient and a preparation method thereof, the preparation method comprising: S1: prepare 40 parts of Gastrodia elata active peptide, 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 30 parts of green tea extract, 20 parts of licorice extract and 10 parts of aloe extract; S2: Put the basic skin care product and emulsifier into a mixing container, heat and stir until the emulsifier is completely dissolved and a uniform emulsion is formed; S3: Add green tea extract and licorice extract to the mixture of emulsifier and basic skin care products, and stir well; S4: When the temperature of the mixture drops to about 40°C, add Gastrodia elata active peptide and aloe vera extract, and continue stirring until completely mixed; S5: When the mixture is cooled to below 40°C, adding the essential oil; S6: Use a pH meter to detect the pH value of the product, and use an appropriate pH regulator to adjust the pH value to between 4.5 and 5.5; S7: Continue stirring the mixture until the desired texture and consistency are achieved, conduct quality tests on the finished product, including stability testing, skin compatibility testing, and effectiveness testing, fill the prepared skin care product into containers, and seal them.
[0026] Furthermore, S2 specifically includes: Prepare stainless steel containers and sterilize them in advance; Weigh 50 parts of basic skin care products and 20 parts of emulsifiers, and pour them into the prepared stainless steel containers respectively; Place the mixing container on a heating device and set the heating temperature to 70-75°C. During the heating process, use a thermometer to monitor the temperature of the mixture in real time; Stir the mixture using a magnetic stirrer at medium speed; The stirring time is 15-30 minutes; Check the state of the mixture regularly to ensure that the emulsifier has been completely dissolved and there are no particles or stratification. If the emulsifier is not completely dissolved and there are lumps, continue heating and stirring until the emulsion reaches a uniform state; When the emulsifier is completely dissolved and the emulsion is uniform, remove the magnetic stirrer from the heating device; Allow the mixture to cool naturally to 40°C-45°C; When the mixture has cooled to the desired temperature of 40°C-45°C, check the viscosity and homogeneity of the emulsion.
[0027] Furthermore, S3 specifically includes: Green tea extract and licorice extract were added in stages; Gradually increase the stirring speed; Use vacuum degassing equipment to remove air bubbles from the mixture; Check the homogeneity and stability of the emulsion.
[0028] Furthermore, S4 specifically includes: Use temperature control equipment to monitor the temperature of the mixture and ensure that it drops to 40°C. If the temperature is still above 40°C, wait for it to cool down naturally; Slowly add Gastrodia elata active peptide, and then slowly add aloe vera extract after adding Gastrodia elata active peptide; While adding the extract, start the magnetic stirrer to stir at a low speed, and gradually increase the stirring speed to ensure that the extract is fully mixed into the basic skin care product; Observe the color and texture of the mixture and take a small amount of the mixture for further testing to check the uniform distribution of the extract and the stability of the product; Continue stirring until the mixture reaches a completely homogeneous state, stirring time is 15-30 minutes, and check the final state of the mixture.
[0029] Furthermore, S5 specifically includes: Accurately weigh the required amount of essential oil according to the proportion of the recipe; Monitor the temperature of the mixture and let it cool to 25°C-30°C; Use a dropper to add the measured essential oils to the mixture; Start the magnetic stirrer to stir at a low speed and gradually increase the stirring speed; Take a small amount of the mixture to check to ensure that the essential oils are evenly distributed and there is no precipitation or stratification; Fill the final mixture into containers.
[0030] Furthermore, S6 specifically includes: Use standard pH buffer to calibrate the pH meter; Take a product sample, immerse the pH meter probe into the sample, wait for a stable reading, and record the current pH value; Add pH adjuster and check the pH value of the product again using a pH meter until the pH value is between 4.5-5.5.
[0031] Further, the Gastrodia elata extraction step specifically includes: Grinding the Gastrodia elata medicinal material into Gastrodia elata powder; Degreasing with 95% ethanol, at a solid-liquid ratio of 1:10 (g / ml), twice, 1 hour each time, recovering the ethanol, collecting the fat-soluble components and defatted Gastrodia elata powder; The defatted Gastrodia elata powder was extracted with 10% ethanol at 80°C, 20 times the amount, twice, 2 hours each time; The extract was filtered, concentrated to 1:2, refrigerated, and filtered; 1% papain and 0.8% trypsin were added to the filtrate in a volume ratio; Add alcohol to 80% and refrigerate overnight; The supernatant was passed through an ultrafiltration membrane and centrifuged at 90,000 rpm for 20 min to obtain the Gastrodia elata polypeptide; The precipitate is collected, vacuum dried, and crushed into fine powder to obtain Gastrodia elata polysaccharide.
[0032] Embodiment 1: S1: prepare 40 parts of Gastrodia elata active peptide, 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 30 parts of green tea extract, 20 parts of licorice extract and 10 parts of aloe extract; S2: Put the basic skin care product and emulsifier into a mixing container, heat and stir until the emulsifier is completely dissolved and a uniform emulsion is formed; S3: Add green tea extract and licorice extract to the mixture of emulsifier and basic skin care products, and stir well; S4: When the temperature of the mixture drops to 40°C, add Gastrodia elata active peptide and aloe vera extract, and continue stirring until completely mixed; S5: When the mixture is cooled to below 40°C, adding the essential oil; S6: Use a pH meter to detect the pH value of the product, and use an appropriate pH regulator to adjust the pH value to between 4.5 and 5.5; S7: Continue stirring the mixture until the desired texture and consistency are achieved, conduct quality tests on the finished product, including stability testing, skin compatibility testing, and effectiveness testing, fill the prepared skin care product into containers, and seal them.
[0033] Embodiment 2: S1: prepare 20 parts of Gastrodia elata active peptide, 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 10 parts of green tea extract, 25 parts of licorice extract and 5 parts of aloe extract; S2: Put the basic skin care product and emulsifier into a mixing container, heat and stir until the emulsifier is completely dissolved and a uniform emulsion is formed; S3: Add green tea extract and licorice extract to the mixture of emulsifier and basic skin care products, and stir well; S4: When the temperature of the mixture drops to 40°C, add Gastrodia elata active peptide and aloe vera extract, and continue stirring until completely mixed; S5: When the mixture is cooled to below 40°C, adding the essential oil; S6: Use a pH meter to detect the pH value of the product, and use an appropriate pH regulator to adjust the pH value to between 4.5 and 5.5; S7: Continue stirring the mixture until the desired texture and consistency are achieved, conduct quality tests on the finished product, including stability testing, skin compatibility testing, and effectiveness testing, fill the prepared skin care product into containers, and seal them.
[0034] Embodiment three: S1: prepare 50 parts of Gastrodia elata active peptide, 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 10 parts of green tea extract, 30 parts of licorice extract and 20 parts of aloe extract; S2: Put the basic skin care product and emulsifier into a mixing container, heat and stir until the emulsifier is completely dissolved and a uniform emulsion is formed; S3: Add green tea extract and licorice extract to the mixture of emulsifier and basic skin care products, and stir well; S4: When the temperature of the mixture drops to 40°C, add Gastrodia elata active peptide and aloe vera extract, and continue stirring until completely mixed; S5: When the mixture is cooled to below 40°C, adding the essential oil; S6: Use a pH meter to detect the pH value of the product, and use an appropriate pH regulator to adjust the pH value to between 4.5 and 5.5; S7: Continue stirring the mixture until the desired texture and consistency are achieved, conduct quality tests on the finished product, including stability testing, skin compatibility testing, and effectiveness testing, fill the prepared skin care product into containers, and seal them.
[0035] Comparative Example 1: S1: Prepare 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 20 parts of green tea extract, 10 parts of licorice extract and 30 parts of aloe extract; S2: Put the basic skin care product and emulsifier into a mixing container, heat and stir until the emulsifier is completely dissolved and a uniform emulsion is formed; S3: Add green tea extract and licorice extract to the mixture of emulsifier and basic skin care products, and stir well; S4: When the temperature of the mixture drops to 40°C, add the aloe vera extract and continue stirring until completely mixed; S5: When the mixture is cooled to below 40°C, adding the essential oil; S6: Use a pH meter to detect the pH value of the product, and use an appropriate pH regulator to adjust the pH value to between 4.5 and 5.5; S7: Continue stirring the mixture until the desired texture and consistency are achieved, conduct quality tests on the finished product, including stability testing, skin compatibility testing, and effectiveness testing, fill the prepared skin care product into containers, and seal them.
[0036] Table 1 Comparison of properties of loose powders prepared in different embodiments Group Whitening and anti-freckle effect Anti-inflammatory and acne-removing effects Anti-wrinkle and anti-aging effects Moisturizing degree Example 1 good good generally good Example 2 generally generally generally generally Example 3 generally good good good Comparative Example 1 not good generally not good generally Whitening and freckle removal, anti-inflammatory and acne removal, wrinkle removal and anti-aging effect testing A1: Recruit volunteers whose skin type and skin color meet the test requirements, ensure that the volunteers are healthy and have no history of allergies, ensure that the volunteers understand the test process and possible risks, and sign an informed consent form; A2: Use a high-resolution camera to take photos of the volunteers' skin and record the initial condition, including spots and uneven skin color. Use a skin analysis instrument to measure baseline data such as skin pigmentation, skin color uniformity, and skin age. A3: Instruct volunteers to use skin care products correctly according to the recommended dosage and frequency in the product manual, check volunteers' usage regularly, record any side effects or discomfort, take skin photos again after using the product for a period of time, and use skin analysis equipment to measure, compare baseline data with later data, evaluate whitening and freckle removal effects, record changes in spots, skin tone evenness, and wrinkle removal and anti-aging effects, etc.
[0037] A4: Conduct statistical analysis on the collected data, evaluate the significance of the product effect, and write a test report summarizing the test results, including specific data on the effect, feedback from volunteers, any side effects, etc.; A5: Based on the test results, summarize the whitening and freckle removal effects as well as the anti-wrinkle and anti-aging effects of the skin care products, analyze the strengths and weaknesses of the products, and provide product improvement suggestions based on the test results, such as adjusting the formula, instructions for use, etc.
[0038] Moisturizing degree test A1: Use a skin conductivity meter to measure the basic moisture content of the skin and obtain basic data; A2: Perform an initial skin test and record baseline data such as skin moisture content and skin elasticity; A3: Prepare a control group trial and conduct comparative tests. Use the skin care products as directed, record the amount and frequency of use, repeat the test at certain intervals to evaluate the sustainability and changes in the moisturizing effect, measure parameters such as skin moisture content and elasticity to evaluate the actual effect of the skin care products, end the trial after a certain period of time, and conduct a final skin moisture content and elasticity test.
[0039] A4: Compare the data of the initial state and the end state, analyze the effect of skin care products on skin moisturizing, and use statistical tools to evaluate the significance of the results.
[0040] The laboratory test steps for the active ingredients of Gastrodia peptide to scavenge free radicals and inhibit tyrosinase activity are as follows: Prepare gastrodia peptide samples, PBS buffer, DPPH solution, 96-well plates and absorbance meter; According to the concentration gradient of ascorbic acid, the concentration gradient of the gastrodia peptide sample was set; The gastrodia peptide samples were prepared into test solutions of corresponding concentrations, 3 replicate wells were set for each concentration, and 1 background control well was set; Reaction group: the corresponding concentration of sample solution is 500 μL, the corresponding concentration of DPPH ethanol solution is 500 μL; Control group: PBS corresponding concentration is 500 μL, DPPH ethanol solution corresponding concentration is 500 μL; The reaction system was placed at room temperature and protected from light for 20 minutes; After the reaction was completed, 200 μL of the reaction solution was added to a 96-well plate; The absorbance of each well was read at 517 nm using an absorbance meter; Calculate the DPPH free radical scavenging rate; If at a certain test concentration, the clearance rate of Gastrodia peptide after reaction with DPPH is greater than 0% and p<0.05 compared with the negative / solvent control, it means that the sample has the ability to scavenge DPPH free radicals and has an antioxidant effect.
[0041] The steps for testing the antibacterial and anti-inflammatory effects of Gastrodia elata polypeptide compound extract are as follows: The antibacterial rate of the composite extract of Gastrodia elata polypeptides against Staphylococcus aureus and Propionibacterium acnes was obtained by microbroth dilution experiment. The specific operation steps of the experiment are as follows: ① Under aseptic operation conditions, add 100 µL of nutrient broth medium (for Staphylococcus aureus culture) or brain heart infusion broth medium (for Propionibacterium acnes culture) to each well of a 96-well plate; ② Add 100 µL of the composite extract solution of Gastrodia elata polypeptides to the first well, mix it with the culture medium in the well by blowing, then aspirate 100 µL of the suspension and add it to the second well, and do this until the 11th well, then aspirate 100 µL of the suspension from the 11th well and discard it, and do not add the composite extract solution to the 12th well as a positive control; ③ Add 100 µL of the diluted bacterial solution to each well The final concentration of the bacterial solution in each well was 1×106 CFU / mL, and a negative control was set up (containing only the anti-acne prescription extract solution and culture medium at different dilution multiples, with the background OD630 of the Tianma polypeptide extract); ④ After incubation for 24 hours, the OD630 was detected and the bacterial survival rate was calculated. Bacterial survival rate (%) = (OD630 of the drug group - OD630 of the negative control well) / (OD630 of the positive control well - OD630 of the negative control well) × 100%
[14] , antibacterial rate (%) = 100% - bacterial survival rate (%).
[0042] The Gastrodia elata polypeptide compound extract solution was diluted 4 to 256 times to observe their inhibitory effects on Staphylococcus aureus and Propionibacterium acnes. Figure 4It can be seen that the antibacterial rate of the Gastrodia elata polypeptide composite extract solution against Staphylococcus aureus is greater than 90% after being diluted 4 to 8 times, but the antibacterial rate after being diluted 64 times is only 16.11%, and there is almost no antibacterial effect after being diluted 256 times (inhibition rate < 10%). For Propionibacterium acnes, the antibacterial rate of the acne-removing formula extract solution is greater than 90% after being diluted 4 to 16 times, the antibacterial rate after being diluted 64 times is 42.50%, and there is almost no antibacterial effect after being diluted 128 times or more (inhibition rate < 10%).
[0043] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.
[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A skin care product containing Gastrodia elata ingredient and a preparation method thereof, characterized in that: The skin care product is prepared from the following materials: 20-60 parts of gastrodia elata active peptide, 30-80 parts of basic skin care products, 10-30 parts of emulsifier, 10-20 parts of essential oil, 20-40 parts of green tea extract, 10-30 parts of licorice extract and 10-20 parts of aloe extract.
2. The skin care product containing Gastrodia elata component and the preparation method thereof according to claim 1, characterized in that: The skin care product is specifically prepared from the following materials: 40 parts of Gastrodia elata active peptide, 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 30 parts of green tea extract, 20 parts of licorice extract and 10 parts of aloe extract.
3. The skin care product containing Gastrodia elata component and the preparation method thereof according to claim 2, characterized in that: The mass ratio of Gastrodia elata active peptide, green tea extract and licorice extract is 4:3:2, and the Gastrodia elata peptide active ingredients include Gastrodia elata polypeptide and gastrodin.
4. A skin care product containing Gastrodia elata ingredient and a preparation method thereof, characterized in that: The preparation method comprises: S1: prepare 40 parts of Gastrodia elata active peptide, 50 parts of basic skin care products, 20 parts of emulsifier, 10 parts of essential oil, 30 parts of green tea extract, 20 parts of licorice extract and 10 parts of aloe extract; S2: Put the basic skin care product and emulsifier into a mixing container, heat and stir until the emulsifier is completely dissolved and a uniform emulsion is formed; S3: Add green tea extract and licorice extract to the mixture of emulsifier and basic skin care products, and stir well; S4: When the temperature of the mixture drops to 40°C, add Gastrodia elata active peptide and aloe vera extract, and continue stirring until completely mixed; S5: When the mixture is cooled to below 40°C, adding the essential oil; S6: Use a pH meter to detect the pH value of the product, and use an appropriate pH regulator to adjust the pH value to between 4.5 and 5.5; S7: Continue stirring the mixture until the desired texture and consistency are achieved, conduct quality tests on the finished product, including stability testing, skin compatibility testing, and effectiveness testing, fill the prepared skin care product into containers, and seal them.
5. The skin care product containing Gastrodia elata component and the preparation method thereof according to claim 4, characterized in that: S2 specifically includes: Prepare stainless steel containers and sterilize them in advance; Weigh 50 parts of basic skin care products and 20 parts of emulsifiers, and pour them into the prepared stainless steel containers respectively; Place the mixing container on a heating device and set the heating temperature to 70-75°C. During the heating process, use a thermometer to monitor the temperature of the mixture in real time; Stir the mixture using a magnetic stirrer at medium speed; The stirring time is 15-30 minutes; Check the state of the mixture regularly to ensure that the emulsifier has been completely dissolved and there are no particles or stratification. If the emulsifier is not completely dissolved and there are lumps, continue heating and stirring until the emulsion reaches a uniform state; When the emulsifier is completely dissolved and the emulsion is uniform, remove the magnetic stirrer from the heating device; Allow the mixture to cool naturally to 40°C-45°C; When the mixture has cooled to the desired temperature of 40°C-45°C, check the viscosity and homogeneity of the emulsion.
6. The skin care product containing Gastrodia elata component and the preparation method thereof according to claim 5, characterized in that: S3 specifically includes: Green tea extract and licorice extract were added in stages; Gradually increase the stirring speed; Use vacuum degassing equipment to remove air bubbles from the mixture; Check the homogeneity and stability of the emulsion.
7. The skin care product containing Gastrodia elata component and the preparation method thereof according to claim 6, characterized in that: S4 specifically includes: Use temperature control equipment to monitor the temperature of the mixture and ensure that it drops to 40°C. If the temperature is still above 40°C, wait for it to cool down naturally; Slowly add Gastrodia elata active peptide, and then slowly add aloe vera extract after adding Gastrodia elata active peptide; While adding the extract, start the magnetic stirrer to stir at a low speed, and gradually increase the stirring speed to ensure that the extract is fully mixed into the basic skin care product; Observe the color and texture of the mixture and take a small amount of the mixture for further testing to check the uniform distribution of the extract and the stability of the product; Continue stirring until the mixture reaches a completely homogeneous state, stirring time is 15-30 minutes, and check the final state of the mixture.
8. The skin care product containing Gastrodia elata component and the preparation method thereof according to claim 7, characterized in that: S5 specifically includes: Accurately weigh the required amount of essential oil according to the proportion of the recipe; Monitor the temperature of the mixture and let it cool to 25°C-30°C; Use a dropper to add the measured essential oils to the mixture; Start the magnetic stirrer to stir at a low speed and gradually increase the stirring speed; Take a small amount of the mixture to check to ensure that the essential oils are evenly distributed and there is no precipitation or stratification; Fill the final mixture into containers.
9. The skin care product containing Gastrodia elata component and the preparation method thereof according to claim 8, characterized in that: S6 specifically includes: Use standard pH buffer to calibrate the pH meter; Take a product sample, immerse the pH meter probe into the sample, wait for a stable reading, and record the current pH value; Add pH adjuster and check the pH value of the product again using a pH meter until the pH value is between 4.5-5.
5.
10. The skin care product containing Gastrodia elata component and the preparation method thereof according to claim 9, characterized in that: The steps of extracting Gastrodia elata specifically include: Grinding the Gastrodia elata medicinal material into Gastrodia elata powder; Degreasing with 95% ethanol, at a solid-liquid ratio of 1:10 (g / ml), twice, 1 hour each time, recovering the ethanol, collecting the fat-soluble components and defatted Gastrodia elata powder; The defatted Gastrodia elata powder was extracted with 10% ethanol at 80°C, 20 times the amount, twice, 2 hours each time; The extract was filtered, concentrated to 1:2, refrigerated, and filtered; 1% papain and 0.8% trypsin were added to the filtrate in a volume ratio; Add alcohol to 80% and refrigerate overnight; The supernatant was passed through an ultrafiltration membrane and centrifuged at 90,000 rpm for 20 min to obtain the Gastrodia elata polypeptide; The precipitate is collected, vacuum dried, and crushed into fine powder to obtain Gastrodia elata polysaccharide.