Preparation method of active aloe vera leaf water and application thereof

Abstract

The present application relates to the field of cosmetic technology, and more particularly to a preparation method of active aloe vera leaf water and application thereof. The preparation method comprises the following steps: (1) washing and air-drying aloe vera leaves, crushing the aloe vera leaves to obtain a slurry, adding the slurry to a 2xYT culture medium added with a pseudomonas fluorescens fermentation product extract and glucose, sterilizing the slurry, inoculating bacillus smithii liquid to ferment, and obtaining a first fermentation product; (2) sterilizing the first fermentation product of step (1), inoculating bacillus natto liquid to ferment, obtaining a second fermentation product, and centrifuging and sterilizing the second fermentation product to obtain the aloe vera leaf water. The aloe vera leaf water obtained by the method has excellent tyrosinase activity inhibition ability. Further, the aloe vera leaf water enhances the anti-aging effect of the cosmetic by improving the tyrosinase activity inhibition ability.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to a preparation method of active aloe vera leaf water and application thereof. BACKGROUND

[0002] With the increasing improvement of people's living standards, skin problems gradually attract people's attention. Skin is the largest organ of the human body, which is affected by internal and external factors, and is prone to dryness, relaxation, yellowish and deepening of wrinkles and other symptoms. Therefore, anti-aging products have become hot-selling products.

[0003] Aloe (Aloe vera (L.) Burm.f.) is a herbaceous plant of the Aloe family in the Liliales order. Literature records that aloe can be used to make high-quality cosmetics. For a long time, aloe leaf juice has been applied in cosmetics. The prior art CN118717555A A composition with firming and anti-wrinkle effects and application thereof discloses that aloe vera leaf water is used in cosmetics to promote skin cell metabolism and keratinocyte turnover, and has the effects of moisturizing, skin tendering, whitening and brightening. The prior art CN118340705A A repair composition containing aloe vera leaf water and ceramide, and a preparation method and application thereof discloses that aloe vera leaf water has the effects of repair and skin moisturizing. The prior art CN115463073A A spirogyra water mask liquid and a preparation method thereof discloses that aloe vera leaf water, rose water, chondrus crispus extract and spirogyra extract cooperate with each other, so that the mask liquid has the effects of whitening, anti-wrinkle and moisturizing. As can be seen from the above, aloe vera leaf water has broad application prospects in cosmetics. Commonly used extraction methods of aloe vera leaf water include acid extraction, alkali extraction and enzyme extraction. With the development of science and technology, fermentation extraction gradually enters the field of view of scholars. At present, there are problems of low content of key active ingredients in the extraction process of aloe vera leaf water by fermentation technology, which limits its application in the field of cosmetics.

[0004] Therefore, the present application improves the extraction method of aloe vera leaf water to improve its inhibitory ability on tyrosinase activity and accelerate its application in the field of cosmetics. SUMMARY

[0005] In order to overcome the shortcomings of the prior art, the primary purpose of the present application is to provide a preparation method of active aloe vera leaf water, which is simple and easy to operate.

[0006] Another purpose of the present application is to provide an application of active aloe vera leaf water, which has broad application prospects.

[0007] The purpose of the present application is achieved by the following technical solutions:

[0008] A preparation method of active aloe vera leaf water comprises the following steps:

[0009] (1) Aloe vera leaves are washed, dried and crushed to obtain a slurry; the slurry is added to a 2xYT culture medium added with a Pseudoalteromonas ferment product extract and glucose, and sterilized; then Bacillus subtilis strain liquid is added for fermentation to obtain a first fermentation product;

[0010] (2) The first fermentation product of step (1) is inoculated with Bacillus natto strain liquid after sterilization and fermented to obtain a second fermentation product, which is centrifuged and sterilized.

[0011] The Bacillus subtilis strain (MultiShot 550) is preserved in the China Industrial Microbial Culture Collection Center;

[0012] The Bacillus natto strain is preserved in the China Typical Culture Collection Center, and the strain preservation number is CCTCC KB20081433.

[0013] Further, the inoculation amount of the first fermentation product and the Bacillus natto strain liquid in step (2) is 1: (0.05-0.1) by mass ratio; the viable bacterial count of the Bacillus natto strain liquid is (1-5) x 10 7 CFU / mL; the fermentation temperature is 30-40℃, the fermentation time is 2-3d, and the fermentation pH is 6.5-7.

[0014] Further, in the 2xYT culture medium added with the Pseudoalteromonas ferment product extract and glucose in step (1), the mass fraction of the Pseudoalteromonas ferment product extract is 1-2%, and the mass fraction of glucose is 0.5-1%.

[0015] Further, in step (1), the mass ratio of the slurry, the 2xYT culture medium, and the Bacillus subtilis strain liquid is 1: (0.1-0.3): (0.01-0.02); the viable bacterial count of the Bacillus subtilis strain liquid is (1-5) x 10 7 CFU / mL.

[0016] Further, in step (1), the fermentation temperature is 36-37℃, and the fermentation time is 2-3d.

[0017] Further, in step (1), the extraction method of the Pseudoalteromonas ferment product extract is as follows: water, phosphate buffer solution, and glucose solution are mixed in a volume ratio of 1: (0.2-0.3): (0.4-0.6) to obtain a fermentation substrate; Pseudoalteromonas is added to the fermentation substrate and cultured at 25-28℃ for 8-10d; the supernatant is obtained by centrifugation, and freeze-dried to obtain the Pseudoalteromonas ferment product extract.

[0018] Further, the concentration of the phosphate buffer solution is 0.3-0.4 mmol / L, and the concentration of the glucose aqueous solution is 30-50 wt%.

[0019] Further, the aloe vera leaf in step (1) is aloe vera barbadensis leaf.

[0020] The application of the active aloe vera leaf water in the preparation of anti-aging cosmetics.

[0021] Further, the mass percentage of the active aloe vera leaf water in the total mass of the cosmetic is 1-8%.

[0022] The present application has the following effects relative to the prior art:

[0023] (1) The present application obtains aloe vera leaf water by multiple fermentation, specifically, Bacillus smithii is used to ferment aloe vera barbadensis leaf, which helps the aloe vera barbadensis leaf to produce a variety of bioactive substances including antioxidants, enzymes, polysaccharides, etc., and provides rich substrates and suitable environment for subsequent fermentation; Bacillus natto is used for secondary fermentation, which can produce a series of bioactive metabolites, these metabolites can inhibit the active center of tyrosinase, and then affect its activity. Furthermore, the synergistic effect between the metabolites produced by Bacillus natto and the fermentation products of Bacillus smithii enhances the inhibitory capacity of the final fermentation product on tyrosinase activity.

[0024] (2) The present application also adds Pseudoalteromonas ferment product extract and other components in the culture medium for the first fermentation, the Pseudoalteromonas ferment product extract contains a variety of enzymes and other bioactive substances, which can on the one hand speed up the fermentation rate and improve the fermentation efficiency; on the other hand, it can also promote Bacillus smithii to convert the effective components in the serum into more bioactive substances, thereby enhancing the inhibition of tyrosinase. Experimental results show that the aloe vera leaf water obtained by the present application has excellent tyrosinase activity inhibition capacity, reaching 50.78%.

[0025] (3) The aloe vera leaf water obtained by the present application has excellent anti-aging effect, and the skin elasticity parameter R5 difference is above 0.73 and the absolute value of the skin tightening parameter F4 difference is above 0.65 after use. DETAILED DESCRIPTION

[0026] The present application will be further described in conjunction with the following examples, but the embodiments of the present application are not limited thereto. Unless otherwise specified, the reagents, methods and devices used in the present application are conventional reagents, methods and devices in the technical field. Unless otherwise specified, the experimental methods in the following examples are usually carried out according to conventional experimental conditions or according to the experimental conditions suggested by the manufacturer. Unless otherwise specified, the reagents and raw materials used in the present application are commercially available.

[0027] Example 1

[0028] Example 1 provides a preparation method of active aloe vera leaf water, comprising the following steps:

[0029] (1) After washing and air-drying the Aloe vera leaves, the leaves are broken by a tissue homogenizer, and the residue is removed by filtering through a 50 μm nylon screen to obtain a slurry; the slurry is added to a 2xYT culture medium to which a Pseudoalteromonas ferment product extract and glucose have been added, the mass fraction of the Pseudoalteromonas ferment product extract in the culture medium being 1.5%, and the mass fraction of the glucose being 0.6%, and the mixture is sterilized; then, a Bacillus subtilis culture solution with a viable bacterial count of 3x10 7 CFU / mL is inoculated, and the mixture is fermented at 36°C under a 200 rpm vibration for 2.5 days to obtain a first fermentation product.

[0030] (2) The first fermentation product of step (1) is sterilized, and a Bacillus natto culture solution with a viable bacterial count of 3x10 7 CFU / mL is inoculated according to a mass ratio of 1:0.06 between the first fermentation product and the Bacillus natto culture solution, and the mixture is fermented at 36°C and pH 6.7 for 2.5 days to obtain a second fermentation product, which is centrifuged and sterilized.

[0031] The preparation process of the Pseudoalteromonas ferment product extract is as follows:

[0032] Water, 0.32 mmol / L phosphate buffer solution, and 34 wt% glucose aqueous solution are mixed according to a volume ratio of 1:0.25:0.5 to obtain a fermentation substrate, and Pseudoalteromonas is added to the fermentation substrate, which is cultured at 26°C for 9 days, centrifuged to obtain a supernatant, and freeze-dried to obtain the Pseudoalteromonas ferment product extract.

[0033] Example 2

[0034] Example 2 provides a preparation method of active aloe vera leaf water, comprising the following steps:

[0035] (1) After washing and air-drying the Aloe vera leaves, the leaves are broken by a tissue homogenizer, and the residue is removed by filtering through a 50 μm nylon screen to obtain a slurry; the slurry is added to a 2xYT culture medium to which a Pseudoalteromonas ferment product extract and glucose have been added, the mass fraction of the Pseudoalteromonas ferment product extract in the culture medium being 1%, and the mass fraction of the glucose being 0.5%, and the mixture is sterilized; then, a Bacillus subtilis culture solution with a viable bacterial count of 1x10 7The Bacillus smithii bacterial liquid with a CFU / mL of 1.0x108 was fermented for 3 days at 36℃ in an environment with 200 rpm oscillation to obtain a first fermentation product.

[0036] (2) The first fermentation product of step (1) was sterilized, and then inoculated with the Bacillus natto bacterial liquid with a mass ratio of 1:0.05, and a viable bacterial number of 1x107CFU / mL. 7 The Bacillus natto bacterial liquid with a CFU / mL of 1.0x108 was fermented for 3 days at 30℃ in an environment with a pH of 6.5 to obtain a second fermentation product, which was centrifuged and sterilized.

[0037] The preparation process of the Pseudomonas alternanobacter fermentation product extract is as follows:

[0038] Water, 0.3 mmol / L phosphate buffer solution and 30 wt% glucose aqueous solution were mixed in a volume ratio of 1:0.2:0.4 to obtain a fermentation substrate, and Pseudomonas alternanobacter was added to the fermentation substrate, which was cultured at 25℃ for 10 days. The supernatant was obtained by centrifugation, and was freeze-dried to obtain the Pseudomonas alternanobacter fermentation product extract.

[0039] Example 3

[0040] Example 3 provides a preparation method of active aloe vera leaf water, which comprises the following steps:

[0041] (1) The leaves of Aloe barbadensis were washed and dried, and then were crushed by a tissue homogenizer. The residue was removed by filtering through a 50 μm nylon screen to obtain a slurry. The slurry was added to 2xYT culture medium added with the Pseudomonas alternanobacter fermentation product extract and glucose, and the medium was sterilized. The mass ratio of the slurry, the 2xYT culture medium and the Bacillus smithii bacterial liquid was 1:0.3:0.02, the volume fraction of the Pseudomonas alternanobacter fermentation product extract in the medium was 2%, and the volume fraction of glucose was 1%. Then, the Bacillus smithii bacterial liquid with a viable bacterial number of 5x107CFU / mL was inoculated. 7 The Bacillus smithii bacterial liquid with a CFU / mL of 1.0x108 was fermented for 2 days at 37℃ in an environment with 200 rpm oscillation to obtain a first fermentation product.

[0042] (2) The first fermentation product of step (1) was sterilized, and then inoculated with the Bacillus natto bacterial liquid with a mass ratio of 1:0.1, and a viable bacterial number of 5x107CFU / mL. 7 The Bacillus natto bacterial liquid with a CFU / mL of 1.0x108 was fermented for 2 days at 40℃ in an environment with a pH of 7 to obtain a second fermentation product, which was centrifuged and sterilized.

[0043] The preparation process of the Pseudomonas alternanobacter fermentation product extract is as follows:

[0044] A fermentation substrate was prepared by mixing water, 0.4 mmol / L phosphate buffer solution and 50 wt% glucose aqueous solution in a volume ratio of 1:0.3:0.6, and Pseudoalteromonas bacteria were added to the fermentation substrate, which was then cultured at 28°C for 8 days. After centrifugation, the supernatant was obtained and freeze-dried to obtain the product.

[0045] Comparative Example 1

[0046] Comparative Example 1 differs from Example 1 in that:

[0047] The culture medium of Step (1) does not contain Pseudoalteromonas fermentation product extract, and the rest is the same as Example 1.

[0048] Comparative Example 2

[0049] Comparative Example 2 provides a method for preparing aloe vera leaf water, comprising the following steps:

[0050] (1) The leaves of Aloe barbadensis were washed and dried, and then crushed using a tissue homogenizer. The residue was removed by filtering through a 50 μm nylon screen to obtain a slurry. The slurry was added to 2xYT medium containing Pseudoalteromonas fermentation product extract and glucose, and the medium was sterilized. The mass ratio of the slurry, 2xYT medium and Bacillus smithii bacterial solution was 1:0.2:0.015. The mass fraction of Pseudoalteromonas fermentation product extract in the medium was 1.5%, and the mass fraction of glucose was 0.6%. Then, 3x10 7 CFU / mL of Bacillus smithii bacterial solution was inoculated, and the fermentation was carried out at 36°C with 200 rpm shaking for 2.5 days to obtain a first fermentation product.

[0051] (2) The first fermentation product of Step (1) was sterilized, and 3x10 7 CFU / mL of Bacillus smithii bacterial solution was inoculated, and the fermentation was carried out at 36°C and pH 6.7 for 2.5 days to obtain a second fermentation product. After centrifugation and sterilization, the product was obtained.

[0052] Comparative Example 3

[0053] Comparative Example 3 provides a method for preparing aloe vera leaf water, comprising the following steps:

[0054] (1) The aloe vera leaf is washed, dried and then crushed by a tissue homogenizer, and the residue is removed by filtering through a nylon screen with a mesh size of 50 μm to obtain a slurry. The slurry is added to a 2×YT medium to which a fermentation product extract of Pseudoalteromonas fermentans and glucose are added, and the medium is sterilized. Then, the medium is inoculated with a Bacillus natto bacterial liquid with a viable bacterial count of 3×10 7 CFU / mL, and the fermentation is carried out at 36°C in an environment with a pH of 6.7 and at a shaking speed of 200 rpm for 2.5 days to obtain a first fermentation product.

[0055] (2) The first fermentation product obtained in step (1) is sterilized, and then inoculated with a Bacillus natto bacterial liquid with a viable bacterial count of 3×10 7 CFU / mL, and the fermentation is carried out at 36°C in an environment with a pH of 6.7 for 2.5 days to obtain a second fermentation product, which is sterilized by centrifugation.

[0056] Comparative Example 4

[0057] Comparative Example 4 provides a method for preparing an aloe vera leaf water, which comprises the following steps:

[0058] The aloe vera leaf is washed, dried and then crushed by a tissue homogenizer, and the residue is removed by filtering through a nylon screen with a mesh size of 50 μm to obtain a slurry. The slurry is added to a 2×YT medium to which a fermentation product extract of Pseudoalteromonas fermentans and glucose are added, and the medium is sterilized. Then, the medium is inoculated with a Bacillus natto bacterial liquid with a viable bacterial count of 3×10 7 CFU / mL and a Bacillus smithii bacterial liquid with a viable bacterial count of 3×10 7 CFU / mL, and the fermentation is carried out at 36°C in an environment with a pH of 6.7 and at a shaking speed of 200 rpm for 5 days to obtain a fermentation product, which is sterilized by centrifugation.

[0059] Experimental Example

[0060] The tyrosinase activity inhibiting ability of the aloe vera leaf water obtained in Examples 1-3 and Comparative Examples 1-4 is detected, and the specific process is as follows:

[0061] Preparation of a test solution

[0062] The aloe leaf water obtained in Examples 1-3 and Comparative Examples 1-4 was added to water to prepare an aloe leaf water test solution with a mass percentage of 5%.

[0063] Experimental setup and grouping

[0064] Solvent background wells (T1): 110 μL of PBS buffer was added to the 96-well plate;

[0065] Solvent reaction wells (T2): 70 μL of PBS buffer and 40 μL of L-tyrosine solution were added to the 96-well plate;

[0066] Test solution background wells (T3): 40 μL of each test solution and 70 μL of PBS solution were added to each well of the 96-well plate;

[0067] Test solution reaction wells (T4): 40 μL of L-tyrosine solution, 40 μL of each test solution, and 30 μL of PBS solution were added to each well of the 96-well plate.

[0068] Experimental process

[0069] After the 96-well plate was incubated at 37°C for 10 min, 20 μL of tyrosinase solution (200 U / mL) was added to each well. After reaction at 37°C for 5 min, the absorbance value of each well at a wavelength of 475 nm was detected using an enzyme label meter, and the tyrosinase activity inhibition rate of the aloe leaf water obtained in Examples 1-3 and Comparative Examples 1-4 was calculated. The calculation formula for tyrosinase activity inhibition is: inhibition rate (%) = [1-(T4-T3) / (T2-T1)] x 100%, and the experimental results are shown in Table 1.

[0070] Table 1

[0071]

[0072] As can be seen from Table 1, the aloe leaf water obtained in Examples 1-3 has a high tyrosinase activity inhibition rate.

[0073] The preparation method of the aloe leaf water obtained in Comparative Example 1 was changed from that of Examples 1-3, which resulted in a decrease in the tyrosinase activity inhibition rate. As can be seen above, changing the formula of the fermentation medium can affect the tyrosinase activity inhibition ability of aloe leaf water. Further analysis shows that the Pseudoalteromonas ferment product extract contains various enzymes and other bioactive substances. On the one hand, these substances can accelerate the fermentation speed and improve the fermentation efficiency; on the other hand, they can also promote Bacillus smithii to convert the effective components in the serum into more bioactive substances, thereby enhancing the inhibition effect on tyrosinase.

[0074] Compared with Examples 1-3, the aloe vera leaf water obtained by using only one kind of bacteria liquid for fermentation culture in Comparative Example 2-3 and using two kinds of bacteria liquid for fermentation culture in Comparative Example 4 has a significantly reduced ability to inhibit the activity of tyrosinase. As can be seen from the above, changing the fermentation bacteria and the adding time of the fermentation bacteria can affect the ability of the aloe vera leaf water to inhibit the activity of tyrosinase. Further analysis shows that fermentation of Aloe vera barbadensis Miller leaves by Bacillus smithii helps the Aloe vera barbadensis Miller leaves to produce a variety of bioactive substances including antioxidants, enzymes, polysaccharides and the like, and provides rich substrates and a suitable environment for subsequent fermentation; the secondary fermentation using Bacillus natto can produce a series of metabolites with biological activity, which can inhibit the active center of tyrosinase and thus affect the activity of tyrosinase. Furthermore, the synergistic effect between the metabolites produced by Bacillus natto and the fermentation products of Bacillus smithii enhances the ability of the final fermentation product to inhibit the activity of tyrosinase.

[0075] Application Example

[0076] The effects of the serum made of the aloe vera leaf water obtained in Examples 1-3 and Comparative Examples 1-4 are described below.

[0077] A serum, which is made of the following raw materials in the mass percentage: 5% of the aloe vera leaf water, 0.2% of carbomer, 4% of trehalose, 0.8% of squalane, 0.04% of p-hydroxyacetophenone, and the balance of water.

[0078] Application Example 1

[0079] The safety of the serum made of the aloe vera leaf water obtained in Examples 1-3 and Comparative Examples 1-4 is tested, and the process is as follows:

[0080] Volunteers and grouping: 140 volunteers aged 18-45 years old are randomly divided into 7 groups, with a male to female ratio of 1:1.

[0081] 0.020 g of each group of serum is added to the patch tester, and after 24 h, the test object is removed. The skin reactions of the volunteers in each group are observed at 0.5 h, 24 h and 48 h after the test object is removed, respectively. The results are recorded according to the skin grading standard in the “Cosmetic Safety Technology Standard” (2015 edition), and the results of the patch test are judged according to the skin reaction grading standard in Table 2. The results are shown in Table 3.

[0082] Table 2

[0083]

[0084] Table 3

[0085]

[0086] In combination with Table 3, the aloe leaf water obtained by the present application is safe to use and does not irritate the skin.

[0087] Application Example 2

[0088] The anti-aging performance of the serum prepared from the aloe leaf water obtained by Examples 1-3 and Comparative Examples 1-4 was tested, and the process was as follows:

[0089] Volunteers and grouping: 70 volunteers aged 18-45 years old were randomly divided into 7 groups.

[0090] The volunteers evenly applied the serum prepared from the aloe leaf water obtained by Examples 1-3 and Comparative Examples 1-4 to the left half of the face in the morning and evening every day, and the right half of the face was used with an equal amount of water, which was continuously used for 8 weeks, and no other skin care products were used during the test period. Each volunteer was followed up once at 0, 8 weeks. Before each test, the volunteers needed to clean the face with water and sit in a constant temperature and humidity environment (temperature 20℃, humidity 52%) for 30 min, and the skin elasticity tester was used to measure the skin at the tail of the left and right eyes of each volunteer, and the difference between the two was calculated. The present application selected the skin elasticity parameter R5 (elasticity of the rebound part / elasticity of the stretching part) and the tightening parameter F4 (area formed by the maximum stretching amount and time) values as the indicators for measuring the anti-aging performance. Each measurement was repeated 5 times at the tail of the left and right eyes, and the average value was taken as the result, which was shown in Table 4.

[0091] Table 4

[0092]

[0093] The difference in skin elasticity parameter R5 was a positive number, and the greater the difference, the better the skin elasticity; the difference in tightening parameter F4 was a negative number, and the greater the absolute value of the difference, the tighter the skin.

[0094] As shown in Table 4, the serum obtained by the present application has excellent anti-aging effect. Specifically, after using the serum of the present application, the difference in skin elasticity parameter R5 is above 0.73, and the absolute value of the difference in tightening parameter F4 is above 0.65. The anti-aging effect of the serum of Examples 1-3 and Comparative Examples 1-4 is reduced to varying degrees, and the preparation process of the aloe leaf water of Comparative Example 1-4 is adjusted. In combination with the result analysis of the tyrosinase activity inhibition ability of the aloe leaf water, the improvement of the tyrosinase activity inhibition ability of the aloe leaf water enhances the anti-aging effect of the cosmetic.

[0095] The above embodiments are only preferred embodiments of the present application, and cannot be used to limit the scope of protection of the present application. Any non-essential changes and substitutions made by those skilled in the art on the basis of the present application are within the scope of protection of the present application.

Claims

1. A method for preparing active aloe leaf water, characterized in that: It comprises the following steps: (1) After the aloe leaves are washed, dried and crushed, a slurry is obtained; the slurry is added to a 2xYT culture medium added with a Pseudoalteromonas ferment product extract and glucose, and sterilized; then Bacillus smithii liquid is inoculated for fermentation, and a first fermentation product is obtained; (2) The first fermentation product of step (1) is sterilized and inoculated with Bacillus natto liquid for fermentation, and a second fermentation product is obtained, which is centrifuged and sterilized.

2. The method for preparing active aloe leaf water according to claim 1, wherein: The mass ratio of the first fermentation product to Bacillus natto bacterial liquid in step (2) is 1:(0.05-0.1); the viable bacterial count of the Bacillus natto bacterial liquid is (1-5)×10 7 CFU / mL; the fermentation temperature is 30-40℃, the fermentation time is 2-3d, and the fermentation pH is 6.5-7.

3. The method for preparing active aloe leaf water according to claim 1, characterized in that: In the 2xYT culture medium added with the Pseudoalteromonas ferment product extract and glucose in step (1), the mass fraction of the Pseudoalteromonas ferment product extract is 1-2%, and the mass fraction of glucose is 0.5-1%.

4. The method for preparing active aloe leaf water according to claim 1, wherein: The mass ratio of the slurry, 2×YT medium and bacillus subtilis liquid in step (1) is 1:(0.1-0.3):(0.01-0.02); the viable bacterial count of the bacillus subtilis liquid is (1-5)×10 7 CFU / mL.

5. The method for preparing active aloe leaf water according to claim 1, characterized in that: The fermentation temperature in step (1) is 36-37℃, and the fermentation time is 2-3d.

6. The method for preparing active aloe leaf water according to claim 1, characterized in that: The extraction method of the Pseudoalteromonas ferment product extract in step (1) is as follows: water, phosphate buffer solution and glucose aqueous solution are mixed uniformly in a volume ratio of 1: (0.2-0.3): (0.4-0.6) to obtain a fermentation substrate; Pseudoalteromonas is added to the fermentation substrate, and cultured at 25-28℃ for 8-10d; the supernatant is obtained by centrifugation, and freeze-dried.

7. The method for preparing active aloe leaf water according to claim 6, characterized in that: The concentration of the phosphate buffer solution is 0.3-0.4mmol / L, and the concentration of the glucose aqueous solution is 30-50wt%.

8. The method for preparing active aloe leaf water according to claim 1, characterized in that: The aloe leaves in step (1) are Aloe barbadensis leaves.

9. Use of the active aloe leaf water of claim 1 in the preparation of anti-aging cosmetics.

10. Use of the active aloe vera leaf water according to claim 9, for the preparation of anti-aging cosmetic products, characterized by the fact that it is used in a concentration of 0.1-10% by weight. The mass percentage of the active aloe leaf water in the total mass of the cosmetic is 1-8%.

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