Multi-row micro-fluidic chip monomer and chip set

Abstract

The invention belongs to the field of micro-fluidic chips, and particularly relates to a multi-row micro-fluidic chip monomer and a chip set. The micro-fluidic chip monomer comprises a reaction carrier and a plurality of three-way valves integrated on the reaction carrier, and is characterized in that each three-way valve is provided with a first opening, a second opening and a third opening, each air hole in the reaction carrier is connected with the second opening of one three-way valve, and the first opening and the third opening of each three-way valve are located on the same straight line. The first and third opening directions of the three-way valves are vertical to the plane of the reaction carrier, and the three-way valves are arranged in parallel; connection matching structures are arranged on the reaction carriers and are used for connecting the reaction carriers in a laminated manner; the first opening and the third opening of the three-way valve are provided with matched connection structures. All the monomers are sequentially connected through a connection matching structure arranged on the reaction carrier and matching connection structures of the first opening and the third opening of the three-way valve at the air holes in the same position to form a chip group. According to the multi-row micro-fluidic chip provided by the invention, a plurality of micro-fluidic chip monomers can be freely assembled according to actual working requirements of various working scenes and the number of samples, so that simultaneous processing of multiple samples is realized.

Description

Technical Field

[0001] The invention belongs to the field of microfluidic chips, and in particular relates to a multi-connected microfluidic chip monomer and a chip set. Background Art

[0002] Miniaturized microfluidic chip nucleic acid extraction technology can concentrate complex nucleic acid extraction processes on one chip and has become one of the increasingly important tools in the field of nucleic acid extraction.

[0003] The microfluidic nucleic acid extraction chip devices currently in use directly connect a single microfluidic chip to the device and can only process one microfluidic chip (one sample) at a time. The sample processing efficiency is low and it is difficult to meet the actual needs of simultaneous detection of multiple samples. Summary of the invention

[0004] In order to solve the deficiencies of the prior art, the present invention provides a multi-connected microfluidic chip monomer and a chip set, which can simultaneously process multiple samples after being loaded after being connected to a device.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is: A multi-connected microfluidic chip monomer comprises a reaction carrier and a plurality of three-way valves integrated on the reaction carrier, wherein the three-way valve has a first, a second and a third opening, each pore on the reaction carrier is connected to the second opening of a three-way valve, the first and the third openings of the three-way valve are on the same straight line, the first and the third opening directions of the three-way valve are perpendicular to the plane of the reaction carrier, and the three-way valves are arranged in parallel; a connection matching structure is provided on the reaction carrier for stacking and connecting the reaction carriers; the first and the third openings of the three-way valve have a matching connection structure.

[0006] A precision microvalve is provided in the air hole, and the flow direction of gas and liquid in the chip channel is precisely controlled by the microvalve together with the micropump of the host.

[0007] Other settings on the reaction carrier can be conventionally designed according to the needs of the extraction process, for example, a plurality of independent liquid storage chambers, sample addition chambers, a main reaction chamber, a plurality of air holes, a waste liquid chamber and a storage chamber are provided; each independent liquid storage chamber, sample addition chamber and main reaction chamber is connected to one air hole respectively through a microfluidic channel, a plurality of microfluidic channels are integrated on the reaction carrier, and the main reaction chamber is connected to each independent liquid storage chamber, sample addition chamber, waste liquid chamber and storage chamber respectively through the microfluidic channels.

[0008] More specifically, taking the magnetic bead method as an example, there are 5 liquid storage chambers, namely the lysis liquid chamber, the magnetic bead suspension liquid chamber, the washing liquid chamber, the elution liquid chamber, and the buffer liquid chamber; then there are 7 pores.

[0009] On the basis of the above, the present invention further provides a multi-row microfluidic chip group, which includes a plurality of the above-mentioned multi-row microfluidic chip monomers, each monomer is connected in sequence through a connecting and matching structure arranged on a reaction carrier, and a matching connection structure of the first and third openings of a three-way valve at the same position of the pore, thereby forming a chip group with multiple layers of reaction carriers, and the three-way valves at the same position on each reaction carrier are also connected in sequence to form a gas channel.

[0010] During use, after the microfluidic chip is loaded with samples and placed in the microfluidic chip extraction device, the gas source channel of the device is connected to the chip pores through a three-way valve, and the connecting channels between the liquid storage chamber and the pores, the liquid storage chamber and the reaction pool, the liquid adding chamber and the pores, and the liquid adding chamber and the reaction pool. The device can achieve pressure difference and control the liquid flow between the liquid storage chamber and the reaction pool, the liquid adding chamber and the reaction pool, the magnetic bead chamber and the reaction pool, the reaction pool and the waste liquid chamber, and the reaction pool and the storage chamber by micro-controlling the air pressure of each pore, thereby realizing the cleavage, washing, elution, purification and other processes in the nucleic acid extraction process, producing a high-purity nucleic acid final product, which is temporarily stored in the liquid storage chamber of the microfluidic chip, waiting for transfer or detection.

[0011] Each air hole has a built-in precision microvalve that uses positive and negative pressure to achieve liquid drive, which is used to control the precise flow of airflow while preventing cross-flow of gas between different chips.

[0012] A specific connection and matching structure on a monomer reaction carrier of a microfluidic chip can be in the form of a fixed snap-on: for example, there are four snaps on the four corners of each microfluidic chip for fixing the relative position. When several chips need to be assembled, precise positioning can be achieved through the corresponding snaps on the four corners of each microfluidic chip, and they are tightly fastened to each other to form a parallel whole.

[0013] The three-way valve in the present invention is the key to the technology. When the single microfluidic chip is connected to the device, the gas source channel of the device is connected to the chip pores through the improved three-way valve, the valves in the first and second opening directions involved in the connection are automatically opened, and the valves in the third opening direction not involved in the connection are automatically closed. Furthermore, a sealing facility is provided on the three-way valve.

[0014] When the microfluidic nucleic acid extraction chip device needs to process multiple samples at a time, after each sample is added to a single microfluidic chip, multiple microfluidic chip monomers are connected through a three-way valve, all holes involved in the connection are automatically opened, and the third opening hole of the last microfluidic chip that is not involved in the connection is automatically closed. All chips involved in the reaction are smoothly connected in parallel to the microfluidic chip extraction device through the three-way valve, achieving connectivity with the gas source of the device. The sealing facility integrated on the three-way valve can automatically achieve sealing.

[0015] Compared with the prior art, the present invention has the following beneficial effects: the multi-row microfluidic chip provided by the present invention can be freely assembled according to the actual working needs of various working scenarios and the number of samples, so as to realize the simultaneous processing of multiple samples, thus overcoming the disadvantage that the previous equipment can only process one chip (one sample) at a time. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Schematic diagram of a single microfluidic chip of the present invention.

[0017] Figure 2 Schematic diagram of the reaction carrier plane of the microfluidic chip monomer.

[0018] Figure 3 This is a working diagram of a single three-way valve.

[0019] Figure 4 This is a schematic diagram of connecting multiple three-way valves.

[0020] 1: sample loading chamber, 2: air hole, 3: lysis liquid chamber, 4: magnetic bead suspension liquid chamber, 5: washing liquid chamber, 6: elution liquid chamber; 7: buffer liquid chamber; 8: fixing buckle, 9: main reaction chamber; 10: waste liquid chamber; 11: storage chamber; 21: first opening, 22: second opening, 23: third opening, 100: reaction carrier; 200: three-way valve. DETAILED DESCRIPTION

[0021] In order to better understand the technical solution of the present invention, the following is a detailed description with reference to the accompanying drawings, but it should not be construed as limiting the protection scope of the present invention.

[0022] Embodiment 1 A multi-linked microfluidic chip monomer includes a reaction carrier and a plurality of three-way valves integrated on the reaction carrier, the three-way valve has a first, a second, and a third opening, each pore on the reaction carrier is connected to the second opening of a three-way valve, the first and third openings of the three-way valve are on the same straight line, the first and third opening directions of the three-way valve are perpendicular to the plane of the reaction carrier, and the three-way valves are arranged in parallel; a connection matching structure is provided on the reaction carrier for stacking the reaction carriers; the first and third openings of the three-way valve have a matching connection structure; and a microvalve is provided in the pore. The following is an explanation in conjunction with the accompanying drawings.

[0023] Schematic diagram of a multi-connected microfluidic chip unit Figure 1, including a reaction carrier 100 and 7 micro three-way valves 200 integrated and installed outside the 7 air holes of the reaction carrier, each three-way valve is arranged in parallel; the length of the three-way valve is substantially the same as the thickness of the reaction carrier; a connection and matching structure is provided on the reaction carrier for stacking and fixing the reaction carriers, and the three-way valves on each air hole are also connected to each other through the self-provided bayonet. The specific planar schematic diagram of the reaction carrier is shown in FIG. Figure 2 ,, Taking five liquid storage chambers as an example, the reaction carrier 100 has five liquid storage chambers (lysate chamber 3, magnetic bead suspension chamber 4, washing chamber 5, elution chamber 6, buffer chamber 7), sample loading chamber 1, main reaction chamber 9, 7 air holes 2, waste liquid chamber 10, and storage chamber 11; the air holes 2 are provided with microvalves, and the 7 air holes 2 are respectively connected with the lysate chamber 3, magnetic bead suspension chamber 4, washing chamber 5, elution chamber 6, buffer chamber 7, sample loading chamber 1, and main reaction chamber 9 through microfluidic channels; a plurality of microfluidic channels are integrated inside the microfluidic chip, and the main reaction chamber 9 is respectively connected with the liquid storage chambers (lysate chamber 3, magnetic bead suspension chamber 4, washing chamber 5, elution chamber 6, buffer chamber 7), sample loading chamber 1, waste liquid chamber 10, and storage chamber 11 through microfluidic channels. The blue line is the airway passage, and the green line is the liquid passage. The collected sample is added to the sample loading chamber 1, and the sample loading hole is provided with a structure to prevent the sample from flowing back. After the sample is added, the lid is closed; there are 7 air holes 2, each of which is provided with a precision microvalve for controlling the precise flow of the airflow and for switching positive pressure and negative pressure to realize liquid drive; after the sample is added, the lysate in the lysate chamber 3 is pushed by the air pressure and injected into the sample loading chamber 1; there are 4 fixing buckles 8 for fixing the relative position on the 4 corners of each microfluidic chip monomer, and when several chip monomers need to be assembled, the corresponding buckles on the 4 corners of each microfluidic chip are used to achieve precise positioning, and they are tightly buckled to form a parallel whole.

[0024] Figure 3 It is a schematic diagram of a single three-way valve. The three-way valve 200 has a first opening 21, a second opening 22 and a third opening 23. The first opening 21 is an airway inlet, and is a mutually matched connection structure with the third opening 23. The equipment gas source interface structure is the same as the third opening 23, and can smoothly achieve the matching connection with the first opening 21 and the third opening 23. When working, the three-way valve is connected to the equipment gas source through the first opening 21, and the gas flows in from the first opening 21 of the three-way valve; the second opening 22 of the three-way valve is an airway outlet, which is connected to the pores of the microfluidic chip, and the gas flows into the microfluidic chip through the second opening 22 and controls the flow direction of the liquid inside the chip. The arrow indicates the flow direction of the working airflow of a single microfluidic chip. The third opening 23 of the three-way valve is also a gas channel. When a single microfluidic chip is used for nucleic acid extraction, the third opening 23 is automatically closed.

[0025] Figure 4Schematic diagram of the connection of multiple three-way valves. Taking the assembly of three three-way valves 200 as an example, the three-way valves are connected to each other through the matching connection structure of the first opening 21 and the third opening 23; the first opening 21 of the first three-way valve of each group of row chips is connected to the equipment gas source; the second opening 22 of the three-way valve is the airway outlet, which is connected to the internal airway of the microfluidic chip monomer; the third opening 23 of the three-way valve is the airway outlet. When the microfluidic chip is assembled, after the third opening 23 of the previous three-way valve is connected to the first opening 21 of the next three-way valve, the airway outlets of the third openings 23 of the three-way valves from the first to the second to last are automatically opened, while the third opening 23 of the three-way valve of the last microfluidic chip monomer remains closed, thereby realizing the connection between the chips in the row chip group and the equipment gas source through the improved three-way valve; the gas flow direction is as shown in FIG. Figure 4 Indicated by the arrow.

[0026] A microfluidic chip set is provided. Taking the assembly of three microfluidic chip monomers as an example, each microfluidic chip monomer has seven air holes, and seven micro three-way valves are integrated. When the chip is assembled, the reaction carriers 100 of the three microfluidic chip monomers are stacked and connected through the fixing buckle 8. The three-way valves at the same position on each reaction carrier are connected in sequence through the first opening and the third opening to form a group of seven three-way valves. The first opening of the first three-way valve of each group of three-way valves is directly connected to the airway port of the host.

Claims

1. A multi-connected microfluidic chip monomer, comprising a reaction carrier and a plurality of three-way valves integrated on the reaction carrier, characterized in that: The three-way valve has a first, a second and a third opening, each pore on the reaction carrier is connected to the second opening of a three-way valve, the first and third openings of the three-way valve are on the same straight line, the first and third opening directions of the three-way valve are perpendicular to the plane of the reaction carrier, and the three-way valves are arranged in parallel; a connecting and matching structure is provided on the reaction carrier for stacking and connecting the reaction carriers; the first and third openings of the three-way valve have a matching connecting structure.

2. The multi-connected microfluidic chip monomer according to claim 1, characterized in that: A precision microvalve is provided in the air hole, and the flow direction of gas and liquid in the chip channel is precisely controlled by the microvalve together with the micropump of the host.

3. The multi-connected microfluidic chip monomer according to claim 2, characterized in that: The reaction carrier is provided with a plurality of independent liquid storage chambers, sample addition chambers, a main reaction chamber, a plurality of air holes, a waste liquid chamber and a storage chamber; each independent liquid storage chamber, sample addition chamber and main reaction chamber is connected with one air hole respectively through a microfluidic channel, a plurality of microfluidic channels are integrated on the reaction carrier, and the main reaction chamber is connected with each independent liquid storage chamber, sample addition chamber, waste liquid chamber and storage chamber respectively through the microfluidic channels.

4. The multi-connected microfluidic chip monomer according to claim 3, characterized in that: The reaction carrier is provided with 5 independent liquid storage chambers, namely, a lysis liquid chamber, a magnetic bead suspension liquid chamber, a washing liquid chamber, an elution liquid chamber, and a buffer liquid chamber; and the number of the air holes is 7.

5. A multi-row microfluidic chipset, characterized in that: The invention comprises a plurality of multi-row microfluidic chip monomers for nucleic acid extraction as described in claim 1, wherein each monomer is connected in sequence through a connecting and matching structure arranged on a reaction carrier and a matching and connecting structure of the first and third openings of a three-way valve at an air hole at the same position.

6. The multi-row microfluidic chipset according to claim 5, characterized in that: A micro valve is arranged in the air hole.

7. The multi-row microfluidic chipset according to claim 6, characterized in that: The reaction carrier is provided with a plurality of independent liquid storage chambers, sample addition chambers, a main reaction chamber, a plurality of air holes, a waste liquid chamber and a storage chamber; each independent liquid storage chamber, sample addition chamber and main reaction chamber is connected with one air hole respectively through a microfluidic channel, a plurality of microfluidic channels are integrated on the reaction carrier, and the main reaction chamber is connected with each independent liquid storage chamber, sample addition chamber, waste liquid chamber and storage chamber respectively through the microfluidic channels.

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