Pogostemon cablin cuttage culture solution and cultivation method

Abstract

The invention discloses a pogostemon cablin cuttage nutrient solution and a cultivation method. The pogostemon cablin cuttage nutrient solution comprises a rooting solution, a nutrient solution A, a nutrient solution B and a nutrient solution C, the rooting liquid comprises the following components: 0.6-2.0 g / L of naphthylacetic acid; the nutrient solution A is prepared from the following components: 1.0 to 3.0 g / L of monopotassium phosphate; the nutrient solution B is prepared from the following components: 1.5 to 2.5 g / L of urea; the nutrient solution C is prepared from the following components: 0.5 to 2.0 g / L of monopotassium phosphate and 0.5 to 2.0 g / L of organic water-soluble fertilizer, and the organic water-soluble fertilizer is prepared from the following components: fish protein peptide, polyglutamic acid and seaweed essence. By optimizing pogostemon cablin cuttage culture conditions and a culture solution formula, the rooting speed and the survival rate of pogostemon cablin are remarkably increased, the environment adaptability of pogostemon cablin cuttings is improved while the breeding cycle is shortened, large-scale planting and industrial production of pogostemon cablin are facilitated, and the pogostemon cablin cuttage method has good market popularization prospects.

Description

Technical Field

[0001] The invention relates to the technical field of patchouli cutting seedling cultivation, and more particularly to a patchouli cutting culture solution and a cultivation method. Background Art

[0002] Patchouli, also known as thorny grass, wild patchouli, raixiang grass, and big-leaf mint, is a perennial herbaceous patchouli cutting of the genus Patchouli in the Lamiaceae family. It has an upright stem, 0.3-1 meters high, quadrangular, branched, and covered with villi. It is one of the authentic southern medicines. Patchouli is pungent, slightly warm, enters the spleen, stomach, and lung meridians, has aromatic turbidity, stops vomiting, and relieves heat. A series of patchouli Chinese medicine preparations have been developed. Patchouli is not only the main raw material for more than 30 kinds of Chinese patent medicines, but also an important raw material for the pharmaceutical and light chemical industries with its extracted patchouli essential oil.

[0003] The propagation of patchouli is extremely restricted by my country's climatic conditions, and the market demand for patchouli is rising year by year. Therefore, artificial cuttings for patchouli breeding has become the main way to solve the problem of resource supply. Cuttings breeding is a method of raising seedlings by taking advantage of the fact that some parts of certain plants are prone to produce adventitious roots. These parts are treated with patchouli cutting growth regulators and cultured under suitable environmental conditions to promote rooting and sprouting to form new seedlings. Cuttings seedlings can increase the plant's reproduction coefficient, accelerate the breeding process, and maintain the purity of the variety; cuttings seedlings save seeds compared to sowing seedlings, have a short seedling raising time, are easy to manage, have low costs, and can be grown vertically, saving space, and have great market promotion value. Cuttings methods and culture solutions have an important influence on the survival rate of patchouli cuttings breeding, but currently patchouli cuttings breeding has problems such as low survival rate and low seedling quality. Summary of the invention

[0004] In view of the above technical problems, the present invention provides a patchouli cutting culture solution and a cultivation method, which have the characteristics of improving the survival rate of patchouli after cutting and high seedling quality.

[0005] In order to achieve the above objectives, this application adopts the following technical solutions:

[0006] A patchouli cutting culture solution, comprising: rooting solution, nutrient solution A, nutrient solution B and nutrient solution C;

[0007] The rooting solution contains: 0.6-2.0 g / L of naphthaleneacetic acid;

[0008] The components of the nutrient solution A include: 1.0-3.0 g / L of potassium dihydrogen phosphate;

[0009] The components of the nutrient solution B are: urea 1.5-2.5 g / L;

[0010] The components of the nutrient solution C are: 0.5-2.0 g / L of potassium dihydrogen phosphate and 0.5-2.0 g / L of organic water-soluble fertilizer, and the components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

[0011] Naphthaleneacetic acid can promote cell division and expansion as a rooting solution for patchouli cuttings, and induce the formation of adventitious roots. Potassium dihydrogen phosphate in nutrient solution A can not only provide nutrients, but also has a certain antibacterial effect. It can provide the phosphorus and potassium elements required for the growth of patchouli cuttings, promote cell division and root growth, thereby increasing the rooting rate and survival rate of the cuttings, and can also improve the disease resistance and stress resistance of patchouli cuttings, and reduce the occurrence of diseases and insect pests. Urea in nutrient solution B can provide the nitrogen source required for the growth of patchouli cuttings, support cell division and tissue growth, and enhance the activity of soil microorganisms to improve the matrix structure. The combination of potassium dihydrogen phosphate in nutrient solution C and organic water-soluble fertilizers can promote the better absorption and utilization of nutrients by patchouli cuttings, further enhance the stress resistance of patchouli cuttings, and better adapt to various environmental changes. Fish protein peptides in organic water-soluble fertilizers can promote the development of the root system of patchouli cuttings, and enhance the ability of patchouli cuttings to absorb and conduct nutrients. Polyglutamic acid has super hydrophilic and water-retaining properties, which promotes the absorption and utilization of trace elements and nutrients such as phosphorus fertilizers, and enhances the resistance of patchouli cuttings to frost damage, drought and waterlogging, salinity and other stresses and diseases. Seaweed essence can promote the development of taproots, lateral roots, and capillary roots, improve the root system, and thus improve the ability of patchouli cuttings to absorb nutrients.

[0012] Cultivating patchouli cuttings with rooting solution combined with nutrient solution can improve the environmental adaptability of the cuttings and promote the healthy development of the root system. The nutrient solution promotes rooting solution to induce cell division and root growth, increase the rooting rate of the cuttings, and promote patchouli to absorb various nutrients in the nutrient solution, including nitrogen, phosphorus, potassium, trace elements, etc., thereby increasing the survival rate of the cuttings and improving the disease resistance and stress resistance of patchouli cuttings.

[0013] Furthermore, the components of the nutrient solution A also include 0.8-2.5 g / L of a fungicide, and the fungicide is a neutral fungicide and / or an acidic fungicide.

[0014] Potassium dihydrogen phosphate can be mixed with acidic or neutral fungicides, but cannot be mixed with alkaline fungicides. Potassium dihydrogen phosphate can be specifically mixed with fungicides such as chloramphenicol, promethazine, chlorhexidine, etc., which are suitable for preventing and treating various diseases of patchouli cuttings. In the embodiment of the present application, carbendazim is used after being mixed with potassium dihydrogen phosphate. Carbendazim is an acidic broad-spectrum systemic fungicide that can prevent and treat various diseases of various crops. Potassium dihydrogen phosphate is a high-quality phosphorus and potassium fertilizer. The combination of the two can comprehensively promote the growth and health maintenance of patchouli cuttings.

[0015] Furthermore, the rooting solution comprises: 1.0 g / L of α-naphthyl acetic acid;

[0016] The components of the nutrient solution A are: 2.0 g / L potassium dihydrogen phosphate and 1.25 g / L carbendazim;

[0017] The components of the nutrient solution B are: urea 2.0 g / L;

[0018] The components of the nutrient solution C are: 1.0 g / L potassium dihydrogen phosphate and 1.0 g / L organic water-soluble fertilizer, and the components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

[0019] The concentration of naphthylacetic acid has an important influence on the growth of patchouli cuttings. Too low a concentration will not achieve the expected effect, while too high a concentration will harm the growth of patchouli cuttings and even cause the death of patchouli cuttings. 1.0g / L of α-naphthylacetic acid can promote the division and expansion of patchouli cuttings cells, increase cell activity, thereby promoting rooting and enhancing the ability of patchouli cuttings to resist cold, drought and acidic and alkaline soils. In nutrient solution A, a mixture of 2.0g / L of potassium dihydrogen phosphate and 1.25g / L of carbendazim can effectively promote the rooting of patchouli cuttings, prevent diseases, and promote the healthy growth of patchouli cuttings. In nutrient solution B, 2.0g / L of urea can avoid the problem of root burn and ensure that crops can effectively absorb nitrogen. In nutrient solution C, 1.0 g / L of potassium dihydrogen phosphate and 1.0 g / L of organic water-soluble fertilizer are mixed to ensure that patchouli cuttings absorb the required nutrients while avoiding root damage caused by excessive concentration or antagonism between components.

[0020] Furthermore, the rooting solution is prepared by dissolving naphthylacetic acid in a 0.1 mol / L sodium hydroxide solution and then making up the volume with tap water.

[0021] Naphthaleneacetic acid is difficult to dissolve in water. Using sodium hydroxide solution to dissolve it can increase the solubility of naphthaleneacetic acid, improve the absorption and utilization rate of naphthaleneacetic acid by patchouli cuttings, and induce the formation of adventitious roots. Before planting, patchouli cuttings only need to be quickly dipped in rooting liquid, without soaking treatment, and the amount of reagents used is small, which can greatly save time and cost.

[0022] A cultivation method for patchouli cuttings comprises the following steps:

[0023] Planting patchouli cuttings dipped in rooting liquid in a substrate; the rooting liquid contains: 0.6-2.0 g / L α-naphthylacetic acid;

[0024] Before the patchouli cuttings take root, nutrient solution A is sprayed at 60-150 g / mu every 5-7 days; the components of the nutrient solution A are: 1.0-3.0 g / L of potassium dihydrogen phosphate and 0.8-2.5 g / L of carbendazim; after the patchouli cuttings take root, nutrient solution B is sprayed at 60-150 g / mu and nutrient solution C is sprayed at 160-250 g / mu every 5-7 days; the components of the nutrient solution B are: 1.5-2.5 g / L of urea; the components of the nutrient solution C are: 0.5-2.0 g / L of potassium dihydrogen phosphate and 0.5-2.0 g / L of organic water-soluble fertilizer, and the components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid and seaweed essence.

[0025] Dipping patchouli cuttings in naphthaleneacetic acid before rooting can accelerate the rooting speed. Applying nutrient solution A before rooting can effectively promote the rooting of patchouli cuttings, prevent diseases, and promote the healthy growth of patchouli cuttings. After rooting, the patchouli cuttings' demand for nitrogen fertilizer increases sharply. The urea in nutrient solution B can provide the nitrogen source required for the growth of the cuttings, promote their cell division and tissue growth, and enhance the activity of soil microorganisms to improve the matrix structure. The combination of potassium dihydrogen phosphate and organic water-soluble fertilizers in nutrient solution C can promote the better absorption and utilization of nutrients by patchouli cuttings, further enhance the stress resistance of patchouli cuttings, and enhance the ability of patchouli cuttings to adapt to the environment after rooting.

[0026] Furthermore, the cultivation method comprises the following steps:

[0027] Sterilize the patchouli cuttings, dip the cuts into rooting liquid, and plant them in a substrate; the rooting liquid contains: 1.0 g / L α-naphthyl acetic acid;

[0028] Before the patchouli cuttings take root, spray nutrient solution A at 100 g / mu every 6 days; the components of the nutrient solution A are: 2.0 g / L potassium dihydrogen phosphate and 1.25 g / L carbendazim;

[0029] After the patchouli cuttings take root, nutrient solution B is sprayed at 100 ɡ / mu and nutrient solution C is sprayed at 200 ɡ / mu every 6 days; the components of the nutrient solution C are: 1.0 g / L potassium dihydrogen phosphate and 1.0 g / L organic water-soluble fertilizer, and the components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

[0030] The concentration of naphthaleneacetic acid has an important influence on the growth of patchouli cuttings. Too low a concentration may not achieve the expected effect, while too high a concentration may harm the growth of patchouli cuttings or even cause the death of patchouli cuttings. 1.0g / L α-naphthaleneacetic acid can promote the division and expansion of patchouli cuttings cells, increase cell activity, thereby promoting rooting and enhancing the ability of patchouli cuttings to resist cold, drought and acid and alkaline soils. Before rooting, nutrient solution A, 2.0g / L potassium dihydrogen phosphate and 1.25g / L carbendazim mixed can effectively promote the rooting of patchouli cuttings, prevent diseases and promote the healthy growth of patchouli cuttings. After rooting, nutrient solution B and 2.0g / L urea can avoid root burn and ensure that crops effectively absorb nitrogen. After rooting, apply nutrient solution C, a mixture of 1.0g / L potassium dihydrogen phosphate and 1.0g / L organic water-soluble fertilizer, to ensure that the patchouli cuttings absorb the required nutrients while avoiding root damage caused by excessive concentration or antagonism between components.

[0031] Furthermore, the temperature of the matrix is ​​20° C.-30° C., and the water content of the matrix is ​​60%-70%.

[0032] Suitable substrate temperature and humidity have a significant impact on the growth of patchouli cuttings. Too high or too low temperatures will hinder root growth; too high humidity will cause root hypoxia and root rot, while too low humidity will prevent the roots from absorbing enough water.

[0033] Furthermore, before the patchouli cuttings take root, the daytime air humidity is 75%-85% RH; after the patchouli cuttings take root, the daytime air humidity is 55%-65% RH.

[0034] Before rooting, patchouli cuttings need a higher air humidity, which will be reduced after rooting. The appropriate air humidity range is conducive to the normal growth and root development of patchouli cuttings. Too high humidity will reduce the oxygen content in the soil, affect the respiration of the root system, and inhibit the development of the root system; too low humidity will increase the transpiration of the leaves, causing the patchouli cuttings to lose water, which is not conducive to growth.

[0035] Furthermore, the matrix configuration method is: take 3 parts of peat soil, 2 parts of earthworm castings and 1 part of perlite by weight, mix them, and then sterilize them with carbendazim.

[0036] This matrix ratio has the characteristics of complete nutrition, good air permeability and strong water retention. Combined with carbendazim, it can not only kill harmful microorganisms in the soil, but also improve the fertility and tax-bearing capacity of the soil, help improve the structure and air permeability of the soil, and promote the reproduction and activity of beneficial microorganisms.

[0037] Furthermore, the patchouli cuttings have buds and leaves.

[0038] The leaves and buds on the cuttings can produce auxin, which helps the cuttings to take root and grow. The higher the auxin content on the cuttings, the faster the rooting speed and the higher the rooting rate, and the higher the survival rate after planting.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] The present invention significantly improves the rooting speed, cutting survival rate and seedling hardening and transplanting survival rate of patchouli by optimizing the patchouli cutting culture conditions and culture solution formula, wherein the cutting survival rate reaches 99%, the seedling hardening and transplanting survival rate reaches 100%, and the seedling quality is greatly improved. The propagation cycle of patchouli cuttings is shortened, and the rooting liquid is combined with the nutrient solution to cultivate the patchouli cuttings to improve the environmental adaptability of the cuttings and promote the healthy development of the root system. The nutrient solution promotes the rooting liquid to induce cell division and root growth, increase the survival rate of the cuttings, and improve the disease resistance and stress resistance of the patchouli cuttings; the rooting liquid promotes patchouli to absorb various nutrients in the nutrient solution, including nitrogen, phosphorus, potassium, trace elements, etc., and increases the rooting rate of the cuttings. Among them, fish protein peptides, polyglutamic acid and seaweed essence are rich in a variety of rooting active factors and trace elements, which can promote the development of the root system. The water retention of polyglutamic acid increases the affinity of fish protein peptides and seaweed essence to the root system. Fish protein peptides and seaweed essence act together on the roots of the cuttings to improve the ability of patchouli cuttings to absorb and conduct nutrients, support root cell division, promote the development of main roots, lateral roots, and capillary roots, and enhance the ability of patchouli cuttings to resist frost damage, drought, waterlogging, salinity and other stress and disease resistance. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0042] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0043] In the attached figure:

[0044] Figure 1 The patchouli cuttings of Example 1;

[0045] Figure 2 The first day of planting the patchouli cuttings in Example 1;

[0046] Figure 3 The root condition of the patchouli cuttings of Example 1 on the 7th day;

[0047] Figure 4The growth status of the patchouli cuttings of Example 1 on the 7th day;

[0048] Figure 5 The root condition of the patchouli cuttings of Example 1 on the 10th day;

[0049] Figure 6 The growth status of the patchouli cuttings of Example 1 on the 10th day;

[0050] Figure 7 The root condition of the patchouli cuttings of Example 1 on the 25th day;

[0051] Figure 8 The growth status of the patchouli cuttings of Example 1 on the 25th day;

[0052] Fig. 9 The root condition of the patchouli cuttings after hardening and transplanting on the 35th day in Example 1;

[0053] Fig.10 The growth status of the patchouli cuttings after hardening and transplanting on the 35th day in Example 1;

[0054] Fig.11 The growth status of the patchouli cuttings after hardening in Example 2;

[0055] Fig.12 This is the growth condition of patchouli cuttings after hardening in comparative example 1;

[0056] Fig.13 This is the growth condition of the patchouli cuttings after seedling hardening in Comparative Example 3. DETAILED DESCRIPTION

[0057] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Modifications or substitutions made to the methods, steps or conditions of the present invention without departing from the spirit and essence of the present invention are within the scope of protection of the present invention. Unless otherwise specified, the experimental materials, reagents, instruments, etc. used in the examples of the present invention are all commercially available; unless otherwise specified, all technical means in the examples of the present invention are conventional means well known to those skilled in the art.

[0058] The following is a method for preparing the matrix in the embodiments of the present invention and the comparative examples:

[0059] Take 3 parts of peat soil, 2 parts of earthworm castings and 1 part of perlite by weight, mix them evenly, put them in a 32-hole seedling sieve, and water them with 800 times of carbendazim for sterilization.

[0060] Example 1

[0061] S1: Select Patchouli cuttings that are vigorous, free of pests and diseases, and have dense branches and nodes, cut the branches above the middle, cut the cut branches into cuttings with one bud and one leaf, and spray the cuttings with 800 times carbendazim solution for sterilization.

[0062] S2: Preparation of rooting solution: Dissolve 1 g of α-naphthylacetic acid in 20 ml of 0.1 mol / L sodium hydroxide solution, add tap water to make up to 1 L, and mix well to obtain 1 g / L α-naphthylacetic acid rooting solution.

[0063] S3: Planting cuttings: Dip the cut end of the cuttings into the rooting solution and insert it into the substrate about 0.5 cm to ensure that the cuttings are tightly combined with the substrate.

[0064] 1) Nutritional management:

[0065] After the cuttings are inserted into the substrate, spray nutrient solution A at 100 ɡ / mu immediately, and then spray it every 6 days until the cuttings take root. After the cuttings take root, spray nutrient solution B at 100 ɡ / mu and nutrient solution C at 200 ɡ / mu every 6 days.

[0066] The components of nutrient solution A are: 2.0 g / L potassium dihydrogen phosphate and 1.25 g / L carbendazim;

[0067] The components of nutrient solution B are: urea 2.0 g / L;

[0068] The components of nutrient solution C are: 1.0 g / L potassium dihydrogen phosphate and 1.0 g / L organic water-soluble fertilizer. The components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

[0069] 2) Temperature and humidity management:

[0070] Keep the substrate temperature between 20℃ and 30℃. When the temperature is below 15℃, install electric heating wires to increase the substrate temperature. When the temperature is above 30℃, increase ventilation to lower the temperature.

[0071] Before the cuttings take root, the air humidity is kept at about 80% RH during the day, and after germination, the air humidity is kept at about 60% RH during the day. Stop the spray system at night or on rainy days, and keep the leaves of the cuttings moist at any time. The moisture content of the substrate is controlled at 60-70%.

[0072] S4: Hardening and transplanting: Hardening of seedlings begins on the 25th day after planting, and transplanting to the field 10 days after hardening.

[0073] Example 2

[0074] S1: Select Patchouli cuttings that are vigorous, free of pests and diseases, and have dense branches and nodes, cut the branches above the middle, cut the cut branches into cuttings with one bud and one leaf, and spray the cuttings with carbendazim solution for sterilization.

[0075] S2: Preparation of rooting solution: Dissolve 0.6 g of α-naphthylacetic acid in 20 ml of 0.1 mol / L sodium hydroxide solution, add tap water to make up to 1 L, and mix well to obtain 0.6 g / L α-naphthylacetic acid rooting solution.

[0076] S3: Planting cuttings: Dip the cut end of the cuttings into the rooting solution and insert it into the substrate about 0.5 cm to ensure that the cuttings are tightly combined with the substrate.

[0077] 1) Nutritional management:

[0078] After the cuttings are inserted into the substrate, spray nutrient solution A at 150 ɡ / mu immediately, and then spray once every 7 days until the cuttings take root. After the cuttings take root, spray nutrient solution B at 150 ɡ / mu and nutrient solution C at 250 ɡ / mu every 7 days.

[0079] The components of nutrient solution A are: 1.0 g / L potassium dihydrogen phosphate and 0.8 g / L carbendazim;

[0080] The components of nutrient solution B are: urea 1.5 g / L;

[0081] The components of nutrient solution C are: 0.5 g / L potassium dihydrogen phosphate and 0.5 g / L organic water-soluble fertilizer. The components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

[0082] 2) Temperature and humidity management:

[0083] Keep the substrate temperature between 15℃ and 30℃. When the temperature is below 15℃, install electric heating wires to increase the substrate temperature. When the temperature is above 30℃, increase ventilation to lower the temperature.

[0084] Before the cuttings take root, the air humidity is kept at 75%-85% RH during the day, and after germination, the air humidity is kept at 55%-65% RH during the day. Stop the spray system at night or on rainy days, and keep the leaves of the cuttings moist at any time. The moisture content of the substrate is controlled at 60-70%.

[0085] S4: Hardening and transplanting: Hardening of seedlings begins on the 24th day after planting, and transplanting to the field 10 days after hardening.

[0086] Example 3

[0087] S1: Select Patchouli cuttings that are vigorous, free of pests and diseases, and have dense branches and nodes, cut the branches above the middle, cut the cut branches into cuttings with one bud and one leaf, and spray the cuttings with carbendazim solution for sterilization.

[0088] S2: Preparation of rooting solution: Dissolve 2.0 g of α-naphthylacetic acid in 20 ml of 0.2 mol / L sodium hydroxide solution, add tap water to make up to 1 L, and mix well to obtain 2.0 g / L α-naphthylacetic acid rooting solution.

[0089] S3: Planting cuttings: Dip the cut end of the cuttings into the rooting solution and insert it into the substrate about 0.5 cm to ensure that the cuttings are tightly combined with the substrate.

[0090] 1) Nutritional management:

[0091] After the cuttings are inserted into the substrate, spray nutrient solution A at 60 ɡ / mu immediately, and then spray once every 5 days until the cuttings take root. After the cuttings take root, spray nutrient solution B at 60 ɡ / mu and nutrient solution C at 160 ɡ / mu every 5 days.

[0092] The components of nutrient solution A are: 3.0 g / L potassium dihydrogen phosphate and 2.5 g / L carbendazim;

[0093] The components of nutrient solution B are: urea 2.5 g / L;

[0094] The components of nutrient solution C are: 2.0 g / L potassium dihydrogen phosphate and 2.0 g / L organic water-soluble fertilizer. The components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

[0095] 2) Temperature and humidity management:

[0096] Keep the substrate temperature between 20℃ and 30℃. When the temperature is below 15℃, install electric heating wires to increase the substrate temperature. When the temperature is above 30℃, increase ventilation to lower the temperature.

[0097] Before the cuttings take root, the air humidity is kept at 75%-85% RH during the day, and after germination, the air humidity is kept at 55%-65% RH during the day. Stop the spray system at night or on rainy days, and keep the leaves of the cuttings moist at any time. The moisture content of the substrate is controlled at 60-70%.

[0098] S4: Hardening and transplanting: Hardening of seedlings begins on the 26th day after planting, and transplanting to the field 10 days after hardening.

[0099] Comparative Example 1

[0100] The difference between this comparative example and Example 1 is that no nutrition management is performed after the cuttings are planted, and the other steps are the same as Example 1.

[0101] Comparative Example 2

[0102] The difference between this comparative example and Example 1 is that no rooting liquid is used, and the other steps are the same as Example 1.

[0103] Comparative Example 3

[0104] The difference between this comparative example and Example 1 is that no rooting liquid is used and no nutrition management is performed after the cuttings are planted. The other steps are the same as in Example 1.

[0105] Result analysis:

[0106] The survival rate, rooting time and leaf yellowing rate of the patchouli cuttings recorded in the above examples and comparative examples were statistically analyzed, and the statistical results are shown in Table 1.

[0107] Table 1

[0108]

[0109] From Table 1, we can see that:

[0110] (1) The survival rates of the cuttings of Examples 1-3 are all higher than those of Comparative Examples 1-3, indicating that the cultivation method of the present application can significantly improve the survival rate of the cuttings of patchouli cuttings. The comparison between Example 1 and Comparative Examples 1-3 shows that compared with using rooting solution or nutrient solution alone, cultivating patchouli cuttings with rooting solution combined with nutrient solution can improve the environmental adaptability of the cuttings and promote the healthy development of the root system. The nutrient solution promotes the rooting solution to induce cell division and root growth, increase the survival rate of the cuttings, and improve the disease resistance and stress resistance of the patchouli cuttings; the rooting solution promotes patchouli to absorb various nutrients in the nutrient solution, including nitrogen, phosphorus, potassium, trace elements, etc., and increase the rooting rate of the cuttings.

[0111] (2) The rooting time of Examples 1-3 is shorter than that of Comparative Examples 1-3, indicating that the combined use of the rooting solution and the nutrient solution of the present application for cultivating patchouli cuttings can promote their rapid rooting. Although the use of the rooting solution or the nutrient solution alone has a certain rooting promoting effect compared with the blank control group (Comparative Example 3), the rooting speed is far lower than the combination of the two. Before the patchouli cuttings take root, the potassium dihydrogen phosphate and carbendazim in the nutrient solution A are mixed and used to promote the plant's absorption of nutrients, enhance the disease resistance of the patchouli cuttings, and promote naphthaleneacetic acid to quickly regulate the activity of biological enzymes in the cuttings, thereby prompting the plant to take root quickly and produce more roots, and the roots produced are stronger. Figure 1-Figure 7 It can be seen that before hardening and transplanting, the roots and leaves of the patchouli cuttings in Example 1 are in good growth and development condition, the seedling quality is high, and the patchouli cuttings have good environmental adaptability and health condition.

[0112] (3) The survival rates of the seedling hardening and transplanting of Examples 1-3 are all higher than those of Comparative Examples 1-3, indicating that the patchouli cuttings cultivated by the cultivation method of the present application have extremely strong environmental adaptability. Not only can the seedlings be enhanced in their tolerance to seedling hardening, but they can also quickly adapt to adverse environmental conditions on land after being planted, and enhance their resistance to adversities such as low temperature and strong winds. By comparing Example 1 with Comparative Examples 1 and 2, it can be seen that compared with the use of rooting solution or nutrient solution alone, the combination of rooting solution and nutrient solution can synergistically promote the growth and development of patchouli seedlings, and significantly improve the environmental adaptability and disease resistance of patchouli cuttings. After the patchouli cuttings take root, the combined use of nutrient solution B and nutrient solution C can enhance the plant's nutrient absorption and stress resistance. Among them, fish protein peptides, polyglutamic acid, and seaweed essence are rich in a variety of rooting active factors and trace elements, which can promote the growth of a more developed root system. The water retention of polyglutamic acid increases the affinity of fish protein peptides and seaweed essence to the root system. Fish protein peptides and seaweed essence work together on the roots of the cuttings to improve the patchouli cuttings' ability to absorb and conduct nutrients, support root cell division, promote the development of taproots, lateral roots, and capillary roots, and enhance the patchouli cuttings' resistance to frost damage, drought and waterlogging, salinity, and other stresses and diseases. Figure 7-Figure 13 It can be seen that after hardening and transplanting, the root system of the patchouli cuttings in the embodiment is thick, the leaves are bright green, the seedling quality is high, and the growth and development conditions of the roots and leaves of the patchouli cuttings are significantly better than those of the patchouli cuttings in the comparative example.

[0113] Compared with the prior art, the present invention has the following beneficial effects:

[0114] The present invention significantly improves the rooting speed, cutting survival rate and seedling hardening and transplanting survival rate of patchouli by optimizing the patchouli cutting culture conditions and culture solution formula, wherein the cutting survival rate reaches 99%, the seedling hardening and transplanting survival rate reaches 100%, and the seedling quality is greatly improved. The propagation cycle of patchouli cuttings is shortened, and the rooting liquid is combined with the nutrient solution to cultivate the patchouli cuttings to improve the environmental adaptability of the cuttings and promote the healthy development of the root system. The nutrient solution promotes the rooting liquid to induce cell division and root growth, increase the survival rate of the cuttings, and improve the disease resistance and stress resistance of the patchouli cuttings; the rooting liquid promotes patchouli to absorb various nutrients in the nutrient solution, including nitrogen, phosphorus, potassium, trace elements, etc., and increases the rooting rate of the cuttings. Among them, fish protein peptides, polyglutamic acid and seaweed essence are rich in a variety of rooting active factors and trace elements, which can promote the development of the root system. The water retention of polyglutamic acid increases the affinity of fish protein peptides and seaweed essence to the root system. Fish protein peptides and seaweed essence act together on the roots of the cuttings to improve the ability of patchouli cuttings to absorb and conduct nutrients, support root cell division, promote the development of main roots, lateral roots, and capillary roots, and enhance the ability of patchouli cuttings to resist frost damage, drought, waterlogging, salinity and other stress and disease resistance.

[0115] It can be understood that the above embodiments only express the preferred implementation modes of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the patent scope of the present invention. It should be pointed out that, for ordinary technicians in this field, the above technical features can be freely combined without departing from the concept of the present invention, and several deformations and improvements can be made, which all belong to the protection scope of the present invention. Therefore, all equivalent changes and modifications made to the scope of the claims of the present invention should belong to the coverage of the claims of the present invention.

Claims

1. A patchouli cutting culture solution, characterized in that: include: Rooting solution, nutrient solution A, nutrient solution B and nutrient solution C; The rooting solution contains: 0.6-2.0 g / L of naphthaleneacetic acid; The components of the nutrient solution A include: 1.0-3.0 g / L of potassium dihydrogen phosphate; The components of the nutrient solution B are: urea 1.5-2.5 g / L; The components of the nutrient solution C are: 0.5-2.0 g / L of potassium dihydrogen phosphate and 0.5-2.0 g / L of an organic water-soluble fertilizer solution, and the components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

2. The patchouli cutting culture solution according to claim 1, characterized in that: The components of the nutrient solution A also include 0.8-2.5 g / L of a fungicide, and the fungicide is a neutral fungicide and / or an acidic fungicide.

3. The patchouli cutting culture solution according to claim 2, characterized in that: The rooting solution contains: 1.0 g / L α-naphthyl acetic acid; The components of the nutrient solution A are: 2.0 g / L potassium dihydrogen phosphate and 1.25 g / L carbendazim; The components of the nutrient solution B are: urea 2.0 g / L; The components of the nutrient solution C are: 1.0 g / L potassium dihydrogen phosphate and 1.0 g / L organic water-soluble fertilizer, and the components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

4. The patchouli cutting culture solution according to claim 1, characterized in that: The rooting liquid is prepared by dissolving naphthylacetic acid in a 0.1-0.2 mol / L sodium hydroxide solution and then fixing the volume with tap water.

5. A method for cultivating patchouli by cuttings, characterized in that: The following steps are involved: Planting patchouli cuttings dipped in rooting liquid in a substrate; the rooting liquid contains: 0.6-2.0 g / L α-naphthylacetic acid; Before the patchouli cuttings take root, spray nutrient solution A at 60-150 g / mu every 5-7 days; the components of the nutrient solution A are: 1.0-3.0 g / L potassium dihydrogen phosphate and 0.8-2.5 g / L carbendazim; After the patchouli cuttings take root, nutrient solution B is sprayed at 60-150 g / mu and nutrient solution C is sprayed at 160-250 g / mu every 5-7 days; the components of the nutrient solution B are: 1.5-2.5 g / L of urea; the components of the nutrient solution C are: 0.5-2.0 g / L of potassium dihydrogen phosphate and 0.5-2.0 g / L of organic water-soluble fertilizer, and the components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

6. The cultivation method according to claim 5, characterized in that: The following steps are involved: Sterilize the patchouli cuttings, dip the cuts into rooting liquid, and plant them in a substrate; the rooting liquid contains: 1.0 g / L α-naphthyl acetic acid; Before the patchouli cuttings take root, spray nutrient solution A at 100 g / mu every 6 days; the components of the nutrient solution A are: 2.0 g / L potassium dihydrogen phosphate and 1.25 g / L carbendazim; After the patchouli cuttings take root, nutrient solution B is sprayed at 100 ɡ / mu and nutrient solution C is sprayed at 200 ɡ / mu every 6 days; the components of the nutrient solution C are: 1.0 g / L potassium dihydrogen phosphate and 1.0 g / L organic water-soluble fertilizer, and the components of the organic water-soluble fertilizer include: fish protein peptide, polyglutamic acid, and seaweed essence.

7. The cultivation method according to claim 5, characterized in that: The temperature of the matrix is ​​20° C.-30° C., and the water content of the matrix is ​​60%-70%.

8. The cultivation method according to claim 5, characterized in that: Before the patchouli cuttings take root, the daytime air humidity is 75%-85% RH; after the patchouli cuttings take root, the daytime air humidity is 55%-65% RH.

9. The cultivation method according to claim 5, characterized in that: The matrix preparation method is: take 3 parts of peat soil, 2 parts of earthworm castings and 1 part of perlite by weight, mix them, and then sterilize them with carbendazim.

10. The cultivation method according to claim 5, characterized in that: The patchouli cuttings are provided with buds and leaves.

Images