Ultraviolet and visible spectrophotometer and detection method thereof

Abstract

The invention relates to the technical field of photometer detection, in particular to an ultraviolet and visible spectrophotometer and a detection method thereof.The method comprises the steps that reference calibration and blank calibration are sequentially conducted on the ultraviolet and visible spectrophotometer, a to-be-detected sample is placed in a sample chamber of the ultraviolet and visible spectrophotometer, and the ultraviolet and visible spectrophotometer is obtained; turning on a light source to measure the to-be-measured sample, and recording the absorbance value of the to-be-measured sample; drawing an absorbance spectrogram of the to-be-detected sample in the ultraviolet and visible spectrophotometer, and calculating the concentration of the to-be-detected sample according to the standard curve; determining the detection accuracy of the ultraviolet and visible spectrophotometer based on the stray light ratio in the detection process; if the detection accuracy does not meet the requirement, adjusting the light source wavelength of an ultraviolet and visible spectrophotometer; and if the measurement reliability does not meet the requirement, adjusting the size of the diaphragm of the ultraviolet and visible spectrophotometer. According to the invention, the detection accuracy of the ultraviolet and visible spectrophotometer is improved.

Description

Technical Field

[0001] The invention relates to the technical field of photometer detection, and in particular to an ultraviolet visible spectrophotometer and a detection method thereof. Background Art

[0002] In the prior art, UV-visible spectrophotometer is an analytical instrument widely used in chemistry, biology, pharmaceuticals, environment and other fields. The accuracy and reliability of its test results are of great importance. In the actual analysis and detection process, many factors will affect the performance of the instrument, thereby interfering with the accuracy and reliability of the measurement.

[0003] Chinese Patent Publication No.: CN110441237A discloses an immersion type UV-visible spectrophotometer detection head and detection method, the method comprises the following steps: during detection, the light source transmits the UV-visible light to the incident optical fiber connector and then to the incident light reflector through the incident optical fiber, the light is turned 90° and then continues to propagate to the incident light collimator to form parallel light after collimation, reaches the detected liquid through the quartz isolation plate, returns to the detection head through the quartz isolation plate, and then converges and focuses the outgoing light through the outgoing light collimator, and focuses on the outgoing optical fiber after 90° reflection through the outgoing light reflector; in actual application, the detection head is directly placed in the water to be detected, and after the light source reaches a stable state, it can continue to work in the water to achieve the purpose of real-time monitoring; at the same time, its position in the water can be controlled by remote control to achieve multi-point detection; there is a cavity between the two isolation plates, which are connected by a structure on the outside, and the distance between the isolation plates can be adjusted by the guide rail, thereby realizing the change of the optical path of the light in the object to be measured. It can be seen that the immersion UV-visible spectrophotometer detection head and detection method have the problem that due to the peeling of paint on the inner wall of the monochromator, the internal wall produces diffuse reflection of light, causing these stray lights to affect the detection, thereby reducing the detection accuracy of the UV-visible spectrophotometer. Summary of the invention

[0004] To this end, the present invention provides an ultraviolet visible spectrophotometer and a detection method thereof, so as to overcome the problem in the prior art that paint peeling off the inner wall of the monochromator causes the internal wall to diffusely reflect light, causing these stray lights to affect the detection, thereby reducing the detection accuracy of the ultraviolet visible spectrophotometer.

[0005] To achieve the above-mentioned object, the present invention provides a detection method for an ultraviolet-visible spectrophotometer, comprising: performing a reference calibration and a blank calibration on the ultraviolet-visible spectrophotometer in sequence, placing a sample to be tested into a sample chamber of the ultraviolet-visible spectrophotometer, turning on a light source to measure the sample to be tested, and recording the absorbance value of the sample to be tested; drawing an absorbance spectrum of the sample to be tested in the ultraviolet-visible spectrophotometer, and calculating the concentration of the sample to be tested according to a standard curve; obtaining the proportion of stray light in the detection process of the sample to be tested; determining the detection accuracy of the ultraviolet-visible spectrophotometer based on the proportion of stray light in the detection process; if the detection accuracy does not meet the requirements, adjusting the wavelength of the light source of the ultraviolet-visible spectrophotometer, or determining the measurement reliability of the ultraviolet-visible spectrophotometer based on the photometric noise intensity of the ultraviolet-visible spectrophotometer; if the measurement reliability does not meet the requirements, adjusting the aperture size of the ultraviolet-visible spectrophotometer, or adjusting the working voltage of the ultraviolet-visible spectrophotometer based on the air humidity in the sample chamber area.

[0006] Further, determining the detection accuracy of the UV-visible spectrophotometer includes:

[0007] Compare the stray light ratio during the detection process with the preset first ratio;

[0008] If the proportion of stray light in the detection process is greater than the preset first proportion, it is determined that the detection accuracy of the UV-visible spectrophotometer does not meet the requirements.

[0009] Further, determining the measurement reliability of the UV-visible spectrophotometer comprises:

[0010] Comparing the stray light ratio in the detection process with the preset first ratio and the preset second ratio respectively;

[0011] If the proportion of stray light in the detection process is greater than the preset first proportion and less than or equal to the preset second proportion, it is preliminarily determined that the measurement reliability of the ultraviolet visible spectrophotometer does not meet the requirements, and whether the measurement reliability of the ultraviolet visible spectrophotometer meets the requirements is determined based on the photometric noise intensity of the ultraviolet visible spectrophotometer.

[0012] Further, adjusting the wavelength of the light source of the ultraviolet visible spectrophotometer includes:

[0013] Comparing the stray light ratio during the detection process with the preset second ratio;

[0014] If the proportion of stray light in the detection process is greater than a preset second proportion, the wavelength of the light source of the ultraviolet visible spectrophotometer is reduced.

[0015] Furthermore, the reduction range of the wavelength of the light source of the ultraviolet-visible spectrophotometer is determined by the difference between the stray light ratio during the detection process and a preset second ratio.

[0016] Further, adjusting the aperture size of the UV-visible spectrophotometer comprises:

[0017] Comparing the photometric noise intensity of the UV-visible spectrophotometer with a preset first noise intensity and a preset second noise intensity respectively;

[0018] If the photometric noise intensity of the ultraviolet-visible spectrophotometer is greater than the preset first noise intensity, it is determined that the measurement reliability of the ultraviolet-visible spectrophotometer does not meet the requirements;

[0019] If the photometric noise intensity of the ultraviolet visible spectrophotometer is greater than the preset first noise intensity and less than or equal to the preset second noise intensity, reducing the aperture size of the ultraviolet visible spectrophotometer;

[0020] If the photometric noise intensity of the UV-visible spectrophotometer is greater than the preset second noise intensity, it is preliminarily determined that the environmental stability measured by the UV-visible spectrophotometer does not meet the requirements, and whether the environmental stability measured by the UV-visible spectrophotometer meets the requirements is determined based on the air humidity in the sample chamber area.

[0021] Furthermore, the reduction range of the aperture size of the ultraviolet-visible spectrophotometer is determined by the difference between the photometric noise intensity of the ultraviolet-visible spectrophotometer and a preset first noise intensity.

[0022] Furthermore, the operating voltage of the UV-visible spectrophotometer is adjusted, including:

[0023] Compare the air humidity in the sample room area with the preset humidity;

[0024] If the air humidity in the sample chamber area is greater than the preset humidity, it is determined that the environmental stability measured by the UV-visible spectrophotometer does not meet the requirements, and the operating voltage of the UV-visible spectrophotometer is increased.

[0025] Furthermore, the increase range of the working voltage of the UV-visible spectrophotometer is determined by the difference between the air humidity in the sample chamber area and a preset humidity.

[0026] The present invention also provides a UV-visible spectrophotometer, comprising:

[0027] A detection module, used for detecting the sample to be tested, comprising a light source for generating ultraviolet and visible light, a monochromator for selecting light of a specific wavelength, a sample chamber for placing the sample to be tested, and a detector for measuring the intensity of light absorbed by the sample to be tested;

[0028] A data processing module, which is connected to the detection module and is used to generate an absorbance spectrum and calculate the absorbance value of the sample to be tested according to the detected light intensity data;

[0029] A display module, connected to the data processing module, for displaying the absorbance spectrum and the absorbance value;

[0030] A power supply module is respectively connected to the detection module, the data processing module and the display module to provide power supply.

[0031] Compared with the prior art, the beneficial effect of the present invention lies in that the method of the present invention adjusts the wavelength of the light source of the ultraviolet visible spectrophotometer according to the proportion of stray light in the detection process. Due to the peeling of the paint on the inner wall of the monochromator, the internal wall surface produces diffuse reflection of the light, which causes the stray light to affect the detection. By reducing the wavelength of the light source of the ultraviolet visible spectrophotometer, the interference of the stray light can be reduced, thereby reducing the relative influence of the stray light. The aperture size of the ultraviolet visible spectrophotometer is adjusted according to the photometric noise intensity of the ultraviolet visible spectrophotometer. After long-term use, the optical components may age and affect their optical performance, thereby causing photometric noise. The sound increases. By reducing the aperture size of the UV-visible spectrophotometer, the total amount of light that generates noise can be reduced, and some stray light can be blocked from entering the detection system, thereby reducing the photometric noise. The operating voltage of the UV-visible spectrophotometer is adjusted according to the air humidity in the sample chamber area. Since the measured sample solution contains volatile components, the air humidity in the sample cell increases, which may penetrate into the electronic components and cause the amplifier component parameters to drift. By increasing the operating voltage of the UV-visible spectrophotometer, it can be ensured that the signal can be correctly amplified, reducing the impact of component performance changes on the accuracy of the measurement results, and improving the detection accuracy of the UV-visible spectrophotometer.

[0032] Furthermore, the method of the present invention adjusts the wavelength of the light source of the ultraviolet visible spectrophotometer by setting a preset first ratio and a preset second ratio. Due to the peeling of paint on the inner wall of the monochromator, the internal wall surface produces diffuse reflection of light, causing these stray lights to affect the detection. By reducing the wavelength of the light source of the ultraviolet visible spectrophotometer, the interference of stray light can be reduced, thereby reducing the relative influence of stray light, and further improving the detection accuracy of the ultraviolet visible spectrophotometer.

[0033] Furthermore, the method of the present invention adjusts the aperture size of the UV-visible spectrophotometer by setting a preset first noise intensity and a preset second noise intensity. After long-term use, the optical components may age and affect their optical performance, thereby increasing the photometric noise. By reducing the aperture size of the UV-visible spectrophotometer, the total amount of light that generates noise can be reduced, and part of the stray light is blocked from entering the detection system, thereby reducing the photometric noise and further improving the detection accuracy of the UV-visible spectrophotometer.

[0034] Furthermore, the method of the present invention adjusts the working voltage of the UV-visible spectrophotometer by setting a preset humidity. Since the measured sample solution contains volatile components, the air humidity in the sample cell increases, which may penetrate into the electronic components and cause the amplifier component parameters to drift. By increasing the working voltage of the UV-visible spectrophotometer, it can be ensured that the signal can be correctly amplified, reducing the impact of component performance changes on the accuracy of the measurement results, and further improving the detection accuracy of the UV-visible spectrophotometer. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 The overall flow chart of the detection method of the ultraviolet visible spectrophotometer according to the embodiment of the present invention;

[0036] Figure 2 The figure is a block diagram of the overall structure of the UV-visible spectrophotometer according to the embodiment of the present invention;

[0037] Figure 3 It is a logic flow chart of the detection method of the ultraviolet visible spectrophotometer according to an embodiment of the present invention;

[0038] Figure 4 The present invention is a specific flow chart of a process for adjusting the wavelength of a light source of an ultraviolet-visible spectrophotometer in a detection method of an ultraviolet-visible spectrophotometer according to an embodiment of the present invention. DETAILED DESCRIPTION

[0039] In order to make the objects and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0040] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the protection scope of the present invention.

[0041] It should be noted that, in the description of the present invention, terms such as "up", "down", "left", "right", "inside" and "outside" indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings. This is merely for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.

[0042] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0043] See also Figure 1 , Figure 2 , Figure 3 as well as Figure 4 As shown, they are respectively an overall flow chart of the ultraviolet visible spectrophotometer and its detection method according to an embodiment of the present invention, an overall structure block diagram, a logic flow chart, and a specific flow chart of the process of adjusting the wavelength of the light source of the ultraviolet visible spectrophotometer. A detection method of an ultraviolet visible spectrophotometer according to the present invention comprises:

[0044] Step S1, performing a reference calibration and a blank calibration on the UV-visible spectrophotometer in sequence, placing a sample to be tested into a sample chamber of the UV-visible spectrophotometer, turning on a light source to measure the sample to be tested, and recording the absorbance value of the sample to be tested;

[0045] Step S2, drawing an absorbance spectrum of the sample to be tested in the UV-visible spectrophotometer, and calculating the concentration of the sample to be tested according to a standard curve;

[0046] Step S3, obtaining the proportion of stray light during the detection process of the sample to be tested;

[0047] Step S4, determining the detection accuracy of the UV-visible spectrophotometer based on the proportion of stray light during the detection process;

[0048] Step S5, if the detection accuracy does not meet the requirement, adjusting the wavelength of the light source of the ultraviolet visible spectrophotometer, or determining the measurement reliability of the ultraviolet visible spectrophotometer based on the photometric noise intensity of the ultraviolet visible spectrophotometer;

[0049] Step S6: if the measurement reliability does not meet the requirement, the aperture size of the UV-visible spectrophotometer is adjusted, or the operating voltage of the UV-visible spectrophotometer is adjusted based on the air humidity in the sample chamber area.

[0050] Specifically, reference calibration includes wavelength calibration and absorbance calibration.

[0051] Specifically, blank calibration includes solvent blank calibration and instrument blank calibration.

[0052] Specifically, the samples to be tested include organic compounds, cell tissue extracts, and industrial wastewater.

[0053] Specifically, the photometric noise intensity of a UV-visible spectrophotometer is the degree of random fluctuation of the absorbance signal during the measurement process when the instrument is in a stable working state.

[0054] Specifically, the aperture size of a UV-visible spectrophotometer is the size of the opening of a device used to control the light flux in the optical path.

[0055] In implementation, the method of the present invention adjusts the wavelength of the light source of the UV-visible spectrophotometer according to the proportion of stray light in the detection process. Due to the peeling of the paint on the inner wall of the monochromator, the internal wall surface produces diffuse reflection of the light, causing the stray light to affect the detection. By reducing the wavelength of the light source of the UV-visible spectrophotometer, the interference of the stray light can be reduced, thereby reducing the relative influence of the stray light. The aperture size of the UV-visible spectrophotometer is adjusted according to the photometric noise intensity of the UV-visible spectrophotometer. After long-term use, the optical components may age and affect their optical performance, thereby increasing the photometric noise. By reducing the wavelength of the light source of the UV-visible spectrophotometer, the interference of the stray light can be reduced, thereby reducing the relative influence of the stray light. The aperture size of the UV-visible spectrophotometer is adjusted according to the photometric noise intensity of the UV-visible spectrophotometer. After long-term use, the optical components may age and affect their optical performance, thereby increasing the photometric noise. Small UV-visible spectrophotometer aperture size can reduce the total amount of light that generates noise, block part of the stray light from entering the detection system, and thus reduce photometric noise. The operating voltage of the UV-visible spectrophotometer is adjusted according to the air humidity in the sample chamber area. Since the measured sample solution contains volatile components, the air humidity in the sample cell increases, which may penetrate into the electronic components and cause the amplifier component parameters to drift. By increasing the operating voltage of the UV-visible spectrophotometer, it can be ensured that the signal can be correctly amplified, reducing the impact of component performance changes on the accuracy of the measurement results, and improving the detection accuracy of the UV-visible spectrophotometer.

[0056] Specifically, determining the detection accuracy of the UV-visible spectrophotometer includes:

[0057] The stray light and total light intensity during the detection of the sample to be tested are respectively obtained, and the proportion of stray light during the detection process is calculated;

[0058] Comparing the stray light ratio during the detection process with a preset first ratio;

[0059] If the proportion of stray light in the detection process is greater than the preset first proportion, it is determined that the detection accuracy of the UV-visible spectrophotometer does not meet the requirements.

[0060] Specifically, determining the measurement reliability of the UV-visible spectrophotometer includes:

[0061] Comparing the stray light ratio in the detection process with the preset first ratio and the preset second ratio respectively;

[0062] If the proportion of stray light in the detection process is greater than the preset first proportion and less than or equal to the preset second proportion, it is preliminarily determined that the measurement reliability of the ultraviolet visible spectrophotometer does not meet the requirements, and whether the measurement reliability of the ultraviolet visible spectrophotometer meets the requirements is determined based on the photometric noise intensity of the ultraviolet visible spectrophotometer.

[0063] It can be understood that the three intervals corresponding to the preset first proportion and the preset second proportion correspond to three situations respectively:

[0064] The first interval is that the stray light ratio during the detection process is less than or equal to the preset first ratio, and the corresponding situation is: the detection accuracy of the UV-visible spectrophotometer meets the requirements;

[0065] The second interval is when the stray light ratio during the detection process is greater than the preset first ratio and less than or equal to the preset second ratio. The corresponding situation is that after long-term use, the optical element may age, affecting its optical performance, thereby increasing the photometric noise.

[0066] The third interval is when the proportion of stray light during the detection process is greater than the preset second proportion. The corresponding situation is: due to the peeling of paint on the inner wall of the monochromator, the internal wall produces diffuse reflection of light, causing the stray light to affect the detection.

[0067] In practice, the preset first ratio is generally selected in the range of [2%, 4%], and the preset second ratio is generally selected in the range of [5%, 7%].

[0068] Preferably, the preferred embodiment of the preset first proportion is 3%, and the preferred embodiment of the preset second proportion is 6%.

[0069] Specifically, the stray light ratio during the detection process is the ratio of the stray light to the total light intensity during the detection of the sample to be tested.

[0070] In implementation, the method of the present invention determines the detection accuracy of the ultraviolet-visible spectrophotometer by setting a preset first ratio and a preset second ratio, thereby reducing the impact of the decrease in detection stability of the ultraviolet-visible spectrophotometer due to inaccurate determination of the detection accuracy of the ultraviolet-visible spectrophotometer, and further improving the detection accuracy of the ultraviolet-visible spectrophotometer.

[0071] Specifically, adjusting the wavelength of the light source of the ultraviolet visible spectrophotometer includes:

[0072] Comparing the stray light ratio during the detection process with the preset second ratio;

[0073] If the proportion of stray light in the detection process is greater than a preset second proportion, the wavelength of the light source of the ultraviolet visible spectrophotometer is reduced.

[0074] Specifically, the reduction range of the wavelength of the light source of the ultraviolet-visible spectrophotometer is determined by the difference between the stray light ratio during the detection process and the preset second ratio.

[0075] Specifically, when the difference between the stray light ratio in the detection process and the preset second ratio is within 2%, the wavelength of the light source of the UV-visible spectrophotometer is reduced to 0.92 times of the original value; when the difference between the stray light ratio in the detection process and the preset second ratio exceeds 2%, on the basis of being reduced to 0.92 times of the original value, the wavelength of the light source of the UV-visible spectrophotometer is further reduced by 20nm for every 1% exceeding it. For example, the difference between the stray light ratio in the detection process and the preset second ratio is 4%, the current wavelength of the light source of the UV-visible spectrophotometer is 350nm, and the wavelength of the light source of the UV-visible spectrophotometer after reduction is 350×0.92-20×2=282nm.

[0076] Specifically, the wavelength of the light source of the UV-visible spectrophotometer is adjusted to be the wavelength of ultraviolet light, and its unit meaning is nanometer.

[0077] In implementation, the method of the present invention adjusts the wavelength of the light source of the ultraviolet visible spectrophotometer by setting a preset first ratio and a preset second ratio. Due to the peeling of paint on the inner wall of the monochromator, the internal wall surface produces diffuse reflection of light, causing these stray lights to affect the detection. By reducing the wavelength of the light source of the ultraviolet visible spectrophotometer, the interference of stray light can be reduced, thereby reducing the relative influence of stray light, and further improving the detection accuracy of the ultraviolet visible spectrophotometer.

[0078] Specifically, adjusting the aperture size of the UV-visible spectrophotometer includes:

[0079] Get the photometric noise intensity of the UV-visible spectrophotometer;

[0080] Comparing the photometric noise intensity of the UV-visible spectrophotometer with a preset first noise intensity and a preset second noise intensity respectively;

[0081] If the photometric noise intensity of the ultraviolet-visible spectrophotometer is greater than the preset first noise intensity, it is determined that the measurement reliability of the ultraviolet-visible spectrophotometer does not meet the requirements;

[0082] If the photometric noise intensity of the ultraviolet visible spectrophotometer is greater than the preset first noise intensity and less than or equal to the preset second noise intensity, reducing the aperture size of the ultraviolet visible spectrophotometer;

[0083] If the photometric noise intensity of the UV-visible spectrophotometer is greater than the preset second noise intensity, it is preliminarily determined that the environmental stability measured by the UV-visible spectrophotometer does not meet the requirements, and whether the environmental stability measured by the UV-visible spectrophotometer meets the requirements is determined based on the air humidity in the sample chamber area.

[0084] It can be understood that the three intervals corresponding to the preset first noise intensity and the preset second noise intensity correspond to three situations respectively:

[0085] The first interval is that the photometric noise intensity of the ultraviolet visible spectrophotometer is less than or equal to the preset first noise intensity, and the corresponding situation is: the measurement reliability of the ultraviolet visible spectrophotometer meets the requirements;

[0086] The second interval is when the photometric noise intensity of the UV-visible spectrophotometer is greater than the preset first noise intensity and less than or equal to the preset second noise intensity. The corresponding situation is that after long-term use, the optical element may age, affecting its optical performance, thereby causing an increase in photometric noise.

[0087] The third interval is when the photometric noise intensity of the UV-visible spectrophotometer is greater than the preset second noise intensity. The corresponding situation is: since the measured sample solution contains volatile components, the air humidity in the sample cell increases, which may penetrate into the electronic components and cause the amplifier component parameters to drift.

[0088] In practice, the preset first noise intensity is generally selected in the range of [0.001Abs / h, 0.003Abs / h], and the preset second noise intensity is generally selected in the range of [0.004Abs / h, 0.006Abs / h].

[0089] Preferably, the preferred embodiment of the preset first noise intensity is 0.002 Abs / h, and the preferred embodiment of the preset second noise intensity is 0.005 Abs / h.

[0090] Specifically, Abs / h is the unit of photometric noise intensity of UV-visible spectrophotometer, which means absorbance unit per hour.

[0091] In implementation, the method of the present invention determines the measurement reliability of the ultraviolet-visible spectrophotometer by setting a preset first noise intensity and a preset second noise intensity, thereby reducing the impact of the decrease in detection accuracy of the ultraviolet-visible spectrophotometer due to inaccurate determination of the measurement reliability of the ultraviolet-visible spectrophotometer, and further improving the detection accuracy of the ultraviolet-visible spectrophotometer.

[0092] Specifically, the reduction range of the aperture size of the ultraviolet-visible spectrophotometer is determined by the difference between the photometric noise intensity of the ultraviolet-visible spectrophotometer and a preset first noise intensity.

[0093] Specifically, when the difference between the photometric noise intensity of the ultraviolet-visible spectrophotometer and the preset first noise intensity is within 0.002Abs / h, the aperture size of the ultraviolet-visible spectrophotometer is reduced to 0.9 times of the original value; when the difference between the photometric noise intensity of the ultraviolet-visible spectrophotometer and the preset first noise intensity exceeds 0.002Abs / h, on the basis of being reduced to 0.9 times of the original value, the aperture size of the ultraviolet-visible spectrophotometer is further reduced by 0.1mm for each 0.001Abs / h exceeding the original value. For example, when the difference between the photometric noise intensity of the ultraviolet-visible spectrophotometer and the preset first noise intensity is 0.004Abs / h, the current aperture size of the ultraviolet-visible spectrophotometer is 2mm, and the aperture size of the ultraviolet-visible spectrophotometer after reduction is 2×0.9-0.1×2=1.6mm.

[0094] In implementation, the method of the present invention adjusts the aperture size of the UV-visible spectrophotometer by setting a preset first noise intensity and a preset second noise intensity. After long-term use, the optical components may age and affect their optical performance, thereby increasing the photometric noise. By reducing the aperture size of the UV-visible spectrophotometer, the total amount of light that generates noise can be reduced, and part of the stray light is blocked from entering the detection system, thereby reducing the photometric noise and further improving the detection accuracy of the UV-visible spectrophotometer.

[0095] Specifically, adjusting the operating voltage of the UV-visible spectrophotometer includes:

[0096] Obtain the air humidity in the sample room area;

[0097] comparing the air humidity in the sample chamber area with a preset humidity;

[0098] If the air humidity in the sample chamber area is greater than the preset humidity, it is determined that the environmental stability measured by the UV-visible spectrophotometer does not meet the requirements, and the operating voltage of the UV-visible spectrophotometer is increased.

[0099] It can be understood that the two intervals corresponding to the preset humidity correspond to two situations:

[0100] The first interval is that the air humidity in the sample room area is less than or equal to the preset humidity, and the corresponding situation is: the environmental stability measured by the UV-visible spectrophotometer meets the requirements;

[0101] The second interval is when the air humidity in the sample chamber area is greater than the preset humidity. The corresponding situation is: since the measured sample solution contains volatile components, the air humidity in the sample pool increases, which may penetrate into the electronic components and cause the amplifier component parameters to drift.

[0102] In practice, the preset humidity is generally selected in the range of [78%, 82%].

[0103] Preferably, the preset humidity is 80%.

[0104] Specifically, the air humidity in the sample chamber area is considered as relative humidity in this solution.

[0105] In implementation, the method of the present invention determines the environmental stability measured by the UV-visible spectrophotometer by setting a preset humidity, thereby reducing the impact of the decrease in detection accuracy of the UV-visible spectrophotometer due to inaccurate determination of the environmental stability measured by the UV-visible spectrophotometer, and further improving the detection accuracy of the UV-visible spectrophotometer.

[0106] Specifically, the increase range of the working voltage of the UV-visible spectrophotometer is determined by the difference between the air humidity in the sample chamber area and the preset humidity.

[0107] Specifically, when the difference between the air humidity in the sample chamber area and the preset humidity is within 20%, the operating voltage of the UV-visible spectrophotometer is increased to 1.1 times of the original value; when the difference between the air humidity in the sample chamber area and the preset humidity exceeds 20%, on the basis of being increased to 1.1 times of the original value, the operating voltage of the UV-visible spectrophotometer is increased by 3V for every 5% exceeding the original value. For example, when the difference between the air humidity in the sample chamber area and the preset humidity is 30%, the current operating voltage of the UV-visible spectrophotometer is 200V, and the increased operating voltage of the UV-visible spectrophotometer is 200×1.1+3×2=226V.

[0108] In implementation, the method of the present invention adjusts the working voltage of the UV-visible spectrophotometer by setting a preset humidity. Since the measured sample solution contains volatile components, the air humidity in the sample cell increases, which may penetrate into the electronic components and cause the amplifier component parameters to drift. By increasing the working voltage of the UV-visible spectrophotometer, it can be ensured that the signal can be correctly amplified, reducing the impact of component performance changes on the accuracy of the measurement results, and further improving the detection accuracy of the UV-visible spectrophotometer.

[0109] A UV-visible spectrophotometer, comprising:

[0110] A detection module, used for detecting the sample to be tested, comprising a light source for generating ultraviolet and visible light, a monochromator for selecting light of a specific wavelength, a sample chamber for placing the sample to be tested, and a detector for measuring the intensity of light absorbed by the sample to be tested;

[0111] A data processing module, which is connected to the detection module and is used to generate an absorbance spectrum and calculate the absorbance value of the sample to be tested according to the detected light intensity data;

[0112] A display module, connected to the data processing module, for displaying the absorbance spectrum and the absorbance value;

[0113] A power supply module is respectively connected to the detection module, the data processing module and the display module to provide power supply.

[0114] Specifically, the display module is a display screen.

[0115] So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

Claims

1. A detection method of ultraviolet visible spectrophotometer, characterized in that, include: Performing a reference calibration and a blank calibration on the UV-visible spectrophotometer in sequence, placing a sample to be tested into a sample chamber of the UV-visible spectrophotometer, turning on a light source to measure the sample to be tested, and recording the absorbance value of the sample to be tested; Drawing an absorbance spectrum of the sample to be tested in the UV-visible spectrophotometer, and calculating the concentration of the sample to be tested according to the standard curve; Obtain the proportion of stray light during the detection of the sample to be tested; Determine the detection accuracy of the UV-visible spectrophotometer based on the proportion of stray light during the detection process; If the detection accuracy does not meet the requirements, the wavelength of the light source of the UV-visible spectrophotometer is adjusted, or the measurement reliability of the UV-visible spectrophotometer is determined based on the photometric noise intensity of the UV-visible spectrophotometer; If the measurement reliability does not meet the requirements, the aperture size of the UV-visible spectrophotometer is adjusted, or the operating voltage of the UV-visible spectrophotometer is adjusted based on the air humidity in the sample chamber area.

2. The detection method of the UV-visible spectrophotometer according to claim 1, characterized in that: Determining the detection accuracy of the UV-visible spectrophotometer includes: Compare the stray light ratio during the detection process with the preset first ratio; If the proportion of stray light in the detection process is greater than the preset first proportion, it is determined that the detection accuracy of the UV-visible spectrophotometer does not meet the requirements.

3. The detection method of the UV-visible spectrophotometer according to claim 2, characterized in that: Determining the measurement reliability of the UV-visible spectrophotometer, including: Comparing the stray light ratio in the detection process with the preset first ratio and the preset second ratio respectively; If the proportion of stray light in the detection process is greater than the preset first proportion and less than or equal to the preset second proportion, it is preliminarily determined that the measurement reliability of the ultraviolet visible spectrophotometer does not meet the requirements, and whether the measurement reliability of the ultraviolet visible spectrophotometer meets the requirements is determined based on the photometric noise intensity of the ultraviolet visible spectrophotometer.

4. The detection method of the UV-visible spectrophotometer according to claim 3, characterized in that: Adjusting the wavelength of the light source of the ultraviolet visible spectrophotometer comprises: Comparing the stray light ratio during the detection process with the preset second ratio; If the proportion of stray light in the detection process is greater than a preset second proportion, the wavelength of the light source of the ultraviolet visible spectrophotometer is reduced.

5. The detection method of the UV-visible spectrophotometer according to claim 4, characterized in that: The reduction range of the wavelength of the light source of the ultraviolet visible spectrophotometer is determined by the difference between the stray light ratio during the detection process and the preset second ratio.

6. The detection method of the UV-visible spectrophotometer according to claim 3, characterized in that: The aperture size of the UV-visible spectrophotometer is adjusted, including: Comparing the photometric noise intensity of the UV-visible spectrophotometer with a preset first noise intensity and a preset second noise intensity respectively; If the photometric noise intensity of the ultraviolet-visible spectrophotometer is greater than the preset first noise intensity, it is determined that the measurement reliability of the ultraviolet-visible spectrophotometer does not meet the requirements; If the photometric noise intensity of the ultraviolet visible spectrophotometer is greater than the preset first noise intensity and less than or equal to the preset second noise intensity, reducing the aperture size of the ultraviolet visible spectrophotometer; If the photometric noise intensity of the UV-visible spectrophotometer is greater than the preset second noise intensity, it is preliminarily determined that the environmental stability measured by the UV-visible spectrophotometer does not meet the requirements, and whether the environmental stability measured by the UV-visible spectrophotometer meets the requirements is determined based on the air humidity in the sample chamber area.

7. The detection method of the UV-visible spectrophotometer according to claim 6, characterized in that: The reduction range of the aperture size of the ultraviolet-visible spectrophotometer is determined by the difference between the photometric noise intensity of the ultraviolet-visible spectrophotometer and a preset first noise intensity.

8. The detection method of the UV-visible spectrophotometer according to claim 7, characterized in that: The operating voltage of the UV-visible spectrophotometer is adjusted, comprising: Compare the air humidity in the sample room area with the preset humidity; If the air humidity in the sample chamber area is greater than the preset humidity, it is determined that the environmental stability measured by the UV-visible spectrophotometer does not meet the requirements, and the operating voltage of the UV-visible spectrophotometer is increased.

9. The detection method of the UV-visible spectrophotometer according to claim 8, characterized in that: The increase range of the working voltage of the ultraviolet visible spectrophotometer is determined by the difference between the air humidity in the sample chamber area and the preset humidity.

10. An ultraviolet-visible spectrophotometer using the detection method of the ultraviolet-visible spectrophotometer according to any one of claims 1 to 9, characterized in that: include: A detection module, used for detecting the sample to be tested, comprising a light source for generating ultraviolet and visible light, a monochromator for selecting light of a specific wavelength, a sample chamber for placing the sample to be tested, and a detector for measuring the intensity of light absorbed by the sample to be tested; A data processing module, which is connected to the detection module and is used to generate an absorbance spectrum and calculate the absorbance value of the sample to be tested according to the detected light intensity data; A display module, connected to the data processing module, for displaying the absorbance spectrum and the absorbance value; A power supply module is respectively connected to the detection module, the data processing module and the display module to provide power supply.

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