Compound composition for removing pet earwax as well as preparation method and application of compound composition

Abstract

The invention discloses a composite composition for removing pet earwax as well as a preparation method and application of the composite composition. The compound composition is prepared from the following components in parts by weight: 0.5 to 1.5 parts of rhamnolipid, 0.1 to 0.5 part of glucoside compound, 0.01 to 0.1 part of compound enzyme and 90 to 99 parts of solvent, the compound enzyme comprises lysozyme, lipase and protease in a mass ratio of (0.01-1.5): (0.1-1.5): (0.1-1.5). The composite composition provided by the invention has excellent dissolving, wetting and emulsifying effects on the animal ear earwax, and can play an excellent effect of removing the animal ear earwax.

Description

Technical Field

[0001] The invention belongs to the technical field of cleaning compositions, and more specifically, relates to a composite composition for removing pet earwax, a preparation method and application thereof. Background Art

[0002] Pet cleaning and hygiene products are pet necessities derived from pet food and medicines, such as cat litter, deodorant spray, pet body cleaning and pet facial cleaning, etc. Affected by the living environment and space, pet owners pay more and more attention to pet hygiene. For example, they hope that their pets can keep their facial features and hair clean, reduce the risk of disease, and keep their living places clean and odor-free. However, my country's pet cleaning and hygiene products market is not segmented enough, and the technical research and safety of related products need deeper exploration.

[0003] Pet body cleaning mainly includes facial cleaning and hair cleaning. Facial cleaning plays an extremely important role in pet health. If the pet's eyes, ears, mouth and nose are not cleaned and cared for in time, there will be potential risks to the pet's health, which puts higher requirements on facial cleaning products. Pet ear health is one of the key issues that most pet-raising families pay attention to. Ear diseases caused by bacterial, fungal and mite infections frequently occur in pets. The root cause is that pets have not developed a habit of daily ear cleaning.

[0004] For cleaning of pet earwax, one of the existing schemes is to use an oil-soluble system to clean the pet earwax. For example, patent publication number CN103142701A discloses an ear drop oil for mixed infection of pet ear canal and its preparation method, wherein the ear drop oil comprises ciprofloxacin hydrochloride, metronidazole, phoxim, lidocaine, tea tree oil, glycerin and distilled water. The ear drop oil softens ear cerumen by dissolving oil with oil, utilizing the strong lipophilicity and permeability of the active ingredients, and avoids further deposition of oily ears and earwax. It has a significant effect on the prevention and treatment of bacterial and fungal infections and secondary otitis media in the pet ear canal. However, this type of ear drop oil is suitable for the treatment of ear diseases and has a certain irritation. It is not the best choice for daily cleaning of pet earwax. Summary of the invention

[0005] In view of the above-mentioned existing technical problems, the primary purpose of the present invention is to provide a composite composition, which has excellent dissolving, wetting and emulsifying effects on animal ear wax and has excellent effect of removing animal ear wax.

[0006] The second object of the present invention is to provide a method for preparing the composite composition.

[0007] The third object of the present invention is to provide a use of the composite composition in removing animal earwax.

[0008] In order to achieve the above object, the present invention is implemented by the following technical solutions:

[0009] The present invention claims protection for a composite composition, which comprises, by weight: 0.5-1.5 parts of rhamnolipid, 0.1-0.5 parts of glucoside compounds, 0.01-0.1 parts of complex enzymes, and 90-99 parts of solvents; the complex enzymes comprise lysozyme, lipase, and protease in a mass ratio of 0.01-1.5:0.1-1.5:0.1-1.5.

[0010] The present invention provides a composite composition, which is combined with rhamnolipid, glucoside compounds and specific composite enzymes to synergistically improve the effect of removing earwax from the ears of animals. Specifically:

[0011] Rhamnolipids are usually fermented by microorganisms in the form of complex mixtures, and there are many isomeric homologues. They have excellent wetting, emulsifying and decontamination effects on the ear canals of pets; in addition, rhamnolipids can also reduce surface tension and penetrate the ear plaque biofilm, and can quickly disintegrate animal earwax. Furthermore, the glucoside compounds and rhamnolipids in the present invention can synergistically wetting and emulsify, can fully penetrate the ear canal grooves of animals, and anchor the oil interface to peel off earwax. Furthermore, the inventors have found through research that the composite enzyme of a specific composition has a good catalytic dissolution effect on dirt such as ear wax. After it is compounded with glucoside compounds, the decontamination effect is further improved. The catalytic dissolution effect of the composite enzyme on ear wax and the good water solubility of glucoside compounds can promote the disintegrated earwax to be easily thrown out by the animal.

[0012] The present invention adopts rhamnosyl ester, glucoside compound and specific complex enzyme combination, which can play an excellent role of decontamination, wetting and emulsification, and has an excellent effect of removing earwax from animal ears.

[0013] Specifically, the dosage of the rhamnolipid can be 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1.0 parts, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, etc., or the range formed by any of the above values, such as 0.5-0.8 parts, 0.7-0.9 parts, etc., and the present invention is not limited thereto. Specifically, the dosage of the glucoside compound can be 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, 0.45 parts, etc., or the range formed by any of the above values, such as 0.3-0.45 parts, 0.4-0.45 parts, etc., and the present invention is not limited thereto. Specifically, the dosage of the complex enzyme can be 0.02 parts, 0.03 parts, 0.04 parts, 0.05 parts, 0.06 parts, 0.07 parts, 0.08 parts, 0.09 parts, etc., or an interval range formed by any of the above values, such as 0.30-0.04 parts, 0.04-0.08 parts, etc., but the present invention is not limited thereto.

[0014] Preferably, the complex enzyme comprises lysozyme, lipase and protease in a mass ratio of 0.5-1.2:0.5-1.2:0.5-1.2. More preferably, the complex enzyme comprises lysozyme, lipase and protease in a mass ratio of 0.8-1:0.8-1:0.8-1.

[0015] Preferably, the solvent is selected from one or more of water, alcohol solvents (such as 1,3-propylene glycol, ethoxydiglycol, etc.), glycerol polyether solvents, and polyethylene glycol solvents.

[0016] Preferably, in parts by weight, the composite composition comprises: 0.5-1.5 parts of rhamnolipid, 0.1-0.5 parts of glucoside compounds, 0.01-0.1 parts of complex enzymes, and 95-97 parts of water. Preferably, preferably, in parts by weight, the composite composition comprises: 0.5-1.5 parts of rhamnolipid, 0.1-0.5 parts of glucoside compounds, 0.01-0.1 parts of complex enzymes, and 96.45-96.9 parts of water.

[0017] Preferably, the glucoside compound is selected from one or more of decyl glucoside, lauryl glucoside and coconut glucoside. Further preferably, the glucoside compound is decyl glucoside.

[0018] Preferably, the composite composition further comprises, by weight: 0.1-1.5 parts of an oil-control deodorant. Specifically, the amount of the oil-control deodorant can be 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1.0 parts, 1.2 parts, 1.4 parts, etc., or an interval range formed by any of the above values, such as 0.2-0.8 parts, 0.6-1.2 parts, etc., but the present invention is not limited thereto.

[0019] Further preferably, the oil control deodorant is a zinc ion oil control deodorant. Zinc ions can effectively reduce sebum secretion by inhibiting 5α reductase, prevent the rapid deposition of oil and the breeding of bacteria, and then decompose foreign matter produced by pets and reduce sour odors. On the other hand, zinc ions chelate ammonia and hydrogen sulfide molecules through the action of electric charges, and wrap odor factors through the hydrogen bonding action of castor oil or glucose, thereby playing a role in reducing odor. More specifically, the zinc ion oil control deodorant can be a zinc ion oil control deodorant conventionally used in the art, including but not limited to zinc ricinoleate, zinc gluconate, zinc chloride, zinc lactate, zinc maleate, zinc sulfate, etc.

[0020] Preferably, the composite composition further comprises, by weight, 0.1-1.5 parts of anti-inflammatory and antipruritic components. Specifically, the anti-inflammatory and antipruritic components may be used in an amount of 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1.0 parts, 1.2 parts, 1.4 parts, etc., or in an interval range formed by any of the above values, such as 0.4-0.6 parts, 0.2-1.4 parts, etc., but the present invention is not limited thereto.

[0021] Further preferably, the anti-inflammatory and antipruritic components are selected from one or more of bisabolol, dipotassium glycyrrhizinate, and gentian extract.

[0022] Preferably, the gentian extract is extracted by water extraction. Specifically, the extraction temperature is 45-60°C; the extraction time is 1-3 hours; and the number of extractions is 1-6 times.

[0023] Preferably, the composite composition further comprises, by weight, 0.1-1.5 parts of an antibacterial agent. Specifically, the amount of the antibacterial agent may be 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts, 1.0 parts, 1.2 parts, 1.4 parts, etc., or an interval range formed by any of the above values, such as 0.6-1.0 parts, 0.2-1.4 parts, etc., and the present invention is not limited thereto.

[0024] More preferably, the antibacterial agent is selected from one or more of cetylpyridinium chloride, benzalkonium chloride, parachloroxylenol, and antibacterial plant essential oils. More preferably, the antibacterial plant essential oil is selected from one or more of lemongrass oil, neem essential oil, and thymol.

[0025] Preferably, the composite composition further comprises a solubilizer. The solubilizer can be selected from solubilizers conventionally used in the art, including but not limited to PEG-40 hydrogenated castor oil, Tween 20, etc. More specifically, the solubilizer comprises, by weight, 0.1-0.5 parts of PEG-40 hydrogenated castor oil and 0.5-1 parts of Tween 20.

[0026] Furthermore, the present invention claims protection for a method for preparing a composite composition, comprising the following preparation steps: mixing rhamnolipid, glucoside compound, complex enzyme, solvent and other components contained in the system to obtain the composite composition.

[0027] Furthermore, the present invention claims the use of the composite composition in removing earwax of animals.

[0028] Specifically, the composite composition has an excellent cleaning effect on pet earwax, and can be used as an ear cleaning liquid to clean pet earwax. The composite composition is particularly suitable for cleaning earwax of pets such as dogs, cats, etc. that have higher requirements for earwax cleaning effects.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention provides a composite composition, which can play an excellent wetting, emulsifying and decontamination role by adopting rhamnolipids, glucoside compounds, and a specific composite enzyme combination, and then matching other components in the system. It can fully penetrate the ear canal grooves of animals, anchor the oil and fat interface, and peel off earwax, thereby synergistically improving the removal effect of earwax in the ears of animals. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is the appearance of the pigskin before testing.

[0032] Figure 2 Pigskin that has been smeared with simulated pet ear wax and colored.

[0033] Figure 3 This is the appearance of the pigskin after the test.

[0034] Figure 4 This is the appearance picture of the pet experiment. DETAILED DESCRIPTION

[0035] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0036] Lysozyme, Delvo Zyme G, DSM (China) Co., Ltd.

[0037] Lipase, LL100, Evonik Specialty Chemicals (Shanghai) Co., Ltd.

[0038] Proteases, 8000, Evonik Specialty Chemicals (Shanghai) Co., Ltd.

[0039] Lemongrass oil, Gezhi Chemical Technology Co., Ltd.

[0040] Neem essential oil, Gezhi Chemical Technology Co., Ltd.

[0041] Thymol, Gezhi Chemical Technology Co., Ltd.

[0042] Rhamnolipid, Evonik Specialty Chemicals (Shanghai) Co., Ltd., trade name RL100.

[0043] The extraction method of gentian extract is as follows: cut 50g of gentian root and grind it into coarse powder, add 200g of boiling water each time to cover the root and cook at 55℃ for 2 hours, pour out the extract, repeat the extraction 3 times, boil for 1 hour, repeat the extraction 2 times, combine the extracts and filter, and concentrate under reduced pressure to obtain a thick liquid, thereby obtaining the gentian extract.

[0044] Example 1 Composite composition

[0045] The invention discloses a composite composition comprising, by weight: 1.5 parts of rhamnolipid, 0.3 parts of decyl glucoside, 0.05 parts of complex enzyme, 0.1 parts of cetylpyridinium chloride, 0.2 parts of neem essential oil, 0.1 parts of zinc ricinoleate, 0.5 parts of zinc gluconate, 0.2 parts of bisabolol, 96.45 parts of water, 0.1 parts of PEG-40 hydrogenated castor oil, and 0.5 parts of Tween 20; in the complex enzyme, the mass ratio of lysozyme, lipase and protease is 1:1:1.

[0046] The composite composition is prepared by the following preparation method: first, water, cetylpyridinium chloride and zinc gluconate are added to a main beaker and stirred to dissolve; rhamnolipid, decyl glucoside, neem essential oil, castor oil zinc oleate and bisabolol are added to another beaker, and 0.1 parts of PEG-40 hydrogenated castor oil and 0.5 parts of Tween 20 are added, and stirred until clear and transparent; then the mixture is poured into the main beaker and stirred to dissolve; finally, a composite enzyme is added and stirred to dissolve to prepare the composite composition.

[0047] Example 2 Composite composition

[0048] In this embodiment, a composite composition includes, by weight: 1.0 part of rhamnolipid, 0.5 part of decyl glucoside, 0.01 part of composite enzyme, 0.1 part of cetylpyridinium chloride, 0.2 part of neem essential oil, 0.05 part of zinc ricinoleate, 0.6 part of zinc gluconate, 0.1 part of dipotassium glycyrrhizinate, 96.84 parts of water, 0.1 part of PEG-40 hydrogenated castor oil, and 0.5 part of Tween 20. In the composite enzyme, the mass ratio of lysozyme, lipase and protease is 1:1:1.

[0049] The preparation method of the composite composition refers to the preparation method in Example 1.

[0050] Example 3 Composite composition

[0051] In this embodiment, a composite composition includes, by weight: 0.5 parts of rhamnolipid, 0.1 parts of decyl glucoside, 0.1 parts of composite enzyme, 0.1 parts of cetylpyridinium chloride, 0.2 parts of neem essential oil, 0.2 parts of zinc ricinoleate, 0.3 parts of zinc gluconate, 1 part of gentian extract, 96.9 parts of water, 0.1 parts of PEG-40 hydrogenated castor oil, and 0.5 parts of Tween 20. In the composite enzyme, the mass ratio of lysozyme, lipase and protease is 1:1:1.

[0052] The preparation method of the composite composition refers to the preparation method in Example 1.

[0053] Comparative Example 1

[0054] The difference between this comparative example and Example 1 is that the composite composition does not contain rhamnolipid.

[0055] Comparative Example 2

[0056] The difference between this comparative example and Example 1 is that the composite composition does not contain decyl glucoside.

[0057] Comparative Example 3

[0058] The difference between this comparative example and Example 1 is that the composite composition does not contain composite enzyme.

[0059] Comparative Example 4

[0060] The difference between this comparative example and Example 1 is that the complex enzyme contains only lysozyme and lipase.

[0061] Comparative Example 5

[0062] The difference between this comparative example and Example 1 is that the complex enzyme contains only lysozyme and protease.

[0063] Comparative Example 6

[0064] The difference between this comparative example and Example 1 is that the complex enzyme contains only lipase and protease.

[0065] Test Example 1: Pet Simulated Earwax Experiment

[0066] Preparation method of pet simulated earwax: Since the main component of pet ear fat is over-secreted oil, 5g of pet bionic sebum was taken to simulate the pet ear fat component (purchased from Dongguan Chuangwei Testing Instrument Co., Ltd.), and a cotton swab was used to wipe cats infected with ear mites (20 cats) to extract the reddish-brown ear mite secretions in the pet ear canal, and then mixed evenly with the pet bionic sebum (5g of pet bionic sebum simulated pet ear fat components were mixed with 0.5g of ear mite secretions) to prepare pet simulated earwax.

[0067] 20 mg of simulated pet earwax was weighed in the experimental beaker, and 15 g of the composite composition prepared in the embodiment and the comparative example was added respectively. After manual shaking for 30 seconds (the shaking frequency was kept consistent each time), the liquid was poured out, and the experimental beaker was placed in a 55°C oven for 24 hours. After returning to room temperature, the weight of the remaining simulated pet earwax in the experimental beaker was weighed. The test results are shown in Table 1 below.

[0068] Table 1

[0069]

[0070]

[0071] As shown in Table 1 above, the composite composition provided by the present invention can play an excellent role in wetting, emulsifying and cleaning pet simulated earwax. The removal rate of the composite composition provided by the present invention for pet simulated earwax is ≥82.5%; more preferably, the removal rate of pet simulated earwax is ≥85%.

[0072] From Comparative Examples 1 to 3, it can be seen that when the composite composition contains only any two components, the removal rate of simulated earwax of pets is ≤56%, and it is difficult to achieve the technical effect of the present invention. From Comparative Examples 4 to 6, it can be seen that when any one of the enzymes in the composite enzyme is missing, the removal rate of simulated earwax of pets is ≤68%, and it is difficult to achieve the technical effect of the present invention.

[0073] Test Example 2: Animal skin simulation use experiment

[0074] Two pieces of pigskin were cut into 3*3 cm squares, and the pet simulated earwax prepared in Test Example 1 was used to simulate the earwax component. 20 mg of pet simulated earwax was applied to each of them (the pet simulated earwax was colored for obvious contrast) and put into a beaker. 10 drops of the composite composition prepared in Example 1 (experimental group) and pure water (control group) were dropped into each of them. After shaking for 10 seconds, the pigskin was taken out, wiped dry with absorbent paper, and weighed. The weight of the pigskin before and after the test was calculated.

[0075] Figure 1 This is the appearance of the pigskin before the test. The pigskin on the left is the experimental group, and the pigskin on the right is the control group.

[0076] Figure 2 Pigskin that has been smeared with simulated pet earwax and colored. The pigskin on the left is the experimental one, and the pigskin on the right is the control one.

[0077] Figure 3 This is the appearance of the pigskin after the test. Figure 3 The pigskin on the left is the experimental group, and the pigskin on the right is the control group. Figure 3It can be seen that the dirt (pet simulated earwax) on the surface of the pigskin in the experimental group has been significantly removed, while the control group still contains a lot of pet simulated earwax. By testing the weight of the pigskin in the experimental and control groups before and after the test, the pet simulated earwax in the pigskin of the experimental group after the test was only 1.8 mg, while the pet simulated earwax in the pigskin of the control group after the test was still 19.2 mg.

[0078] Test Example 3: Pet Experiment

[0079] Test subjects: cats with earwax in their ears. Operation method: pour 5-8 drops of the composite composition prepared in Example 1, gently massage the cat's ears for 30 seconds, release your hands, let the pet shake it out freely, and wipe the earwax out of the external ear canal with a paper towel.

[0080] like Figure 4 As shown, after using the composite composition prepared in Example 1, a large amount of dirt can be clearly seen being thrown out of the external auditory canal of the pet. After gently wiping with a paper towel, the external auditory canal and the deep part of the cochlea are clean and free of obvious foreign matter. The above experimental phenomenon shows that the composite composition provided by the present invention can fully penetrate the ear canal grooves of the animal, anchor the oil interface, peel off the earwax, and achieve the effect of removing earwax from the animal's ear.

[0081] The foregoing examples are merely illustrative and are used to explain some features of the method of the present invention. The appended claims are intended to require the widest possible range that can be imagined, and the embodiments presented herein are demonstrated by the applicant's actual test results. Therefore, the applicant's intention is that the appended claims are not limited by the selection of examples that illustrate the features of the present invention. Some numerical ranges used in the claims also include sub-ranges therein, and changes in these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. A composite composition, characterized in that The composition comprises, by weight: 0.5-1.5 parts of rhamnolipid, 0.1-0.5 parts of glucoside compound, 0.01-0.1 parts of complex enzyme, and 90-99 parts of solvent; The complex enzyme comprises lysozyme, lipase and protease in a mass ratio of 0.01-1.5:0.1-1.5:0.1-1.

5.

2. The composite composition according to claim 1, characterized in that: The complex enzyme comprises lysozyme, lipase and protease in a mass ratio of 0.5-1.2:0.5-1.2:0.5-1.

2.

3. The composite composition according to claim 1, characterized in that: Calculated by weight, the invention comprises: 0.5-1.5 parts of rhamnolipid, 0.1-0.5 parts of glucoside compound, 0.01-0.1 parts of complex enzyme and 95-97 parts of water.

4. The composite composition according to claim 1, characterized in that: The glucoside compound is selected from one or more of decyl glucoside, lauryl glucoside and coconut glucoside.

5. The composite composition according to claim 1, characterized in that: Calculated by weight, it also includes: 0.1-1.5 parts of oil control deodorant.

6. The composite composition according to claim 5, characterized in that: The oil-control deodorant is a zinc ion oil-control deodorant.

7. The composite composition according to claim 1, characterized in that: By weight, it also includes 0.1-1.5 parts of anti-inflammatory and antipruritic components.

8. The composite composition according to claim 7, characterized in that: The anti-inflammatory and antipruritic components are selected from one or more of bisabolol, dipotassium glycyrrhizinate, and gentian extract.

9. The method for preparing the composite composition according to any one of claims 1 to 8, characterized in that: The method comprises the following preparation steps: mixing rhamnolipid, glucoside compound, complex enzyme, solvent and other components contained in the system to obtain the complex composition.

10. Use of the composite composition according to any one of claims 1 to 8 in removing earwax from animals.

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