Preparation method of self-adaptive rhizoma polygonati component extracting solution

Abstract

The invention relates to the technical field of rhizoma polygonati extraction, in particular to a preparation method of a self-adaptive rhizoma polygonati component extracting solution. The method comprises the following steps: cleaning and steaming; determining a steaming end point; performing concentration treatment to obtain extract; mixing the extract with an excipient to obtain a soft material, sieving the soft material, and analyzing the sieving effect; and determining whether to adjust the addition amount of the excipient or the single steaming time according to the screening effect. According to the method, the apparent color change of rhizoma polygonati after each steaming is monitored, and the steaming times are accurately monitored, so that the processing reaches the optimal state, the invalid steaming time is shortened, the steaming end point is intelligently judged, the extraction efficiency is improved, and the product quality is guaranteed; in each steaming process, correlation analysis is carried out on the apparent color change and condensation amount of the rhizoma polygonati to optimize steaming setting parameters, realize adaptive control, maximally extract effective components in the rhizoma polygonati, optimize the production process and ensure the quality and stability of a rhizoma polygonati extracting solution.

Description

Technical Field

[0001] The invention relates to the technical field of polygonatum extraction, and in particular to a method for preparing an adaptive polygonatum component extract. Background Art

[0002] Polygonatum sibiricum is a perennial herbaceous plant of the genus Polygonatum in the monocotyledonous Liliaceae family, including Yunnan Polygonatum sibiricum, Polygonatum sibiricum and Polygonatum sibiricum. It is mainly distributed in East Asia and has been widely used as a food and medicine product for thousands of years. It has immunomodulatory, hypoglycemic, anti-inflammatory, antioxidant, anti-tumor and anti-osteoporosis activities. Raw Polygonatum sibiricum usually has throat irritation, accompanied by tongue numbness, poor taste, and is not conducive to direct consumption. Therefore, Polygonatum sibiricum is often processed and used as medicine, which tastes better.

[0003] As a food pasteurization technology, high-pressure steam sterilization is easy to operate and can inhibit the production of bacteria and fungi at high temperatures. Compared with the traditional method of nine steaming and nine drying, it avoids the contamination of harmful microorganisms and other compounds in the environment, prolongs the shelf life of food, kills food pathogens, removes residual heavy metals such as lead and cadmium, and improves the safety of food and medicine products. High-pressure steam heat processing, as an atypical processing method, is usually used for the processing of meat products to prolong their shelf life, but it is rarely used in the active ingredients of natural products. Therefore, high-temperature steam sterilization is used to heat process Polygonatum sibiricum to achieve batch extraction of the effective ingredients of Polygonatum sibiricum.

[0004] The Chinese patent authorization document with the announcement number CN113786453B discloses a method for preparing a polygonatum extract rich in polygonatum polysaccharides and multiple active ingredients, comprising the following steps: A method for preparing a polygonatum extract rich in polygonatum polysaccharides and multiple active ingredients, comprising the following steps: (1) enzymatic hydrolysis; (2) low-temperature instantaneous extraction: after adding water to the polygonatum enzymatic hydrolyzate obtained in step (1) and mixing evenly, extracting 1-3 times using a high-efficiency high-pressure difference low-temperature continuous extraction device under the conditions of an extraction pressure of 20-40MPa, an extraction temperature of 20-30°C, and an extraction rate of 5-60L / min to obtain a polygonatum extract; (3) separation and purification: centrifuging and microfiltrating the polygonatum extract obtained in step (2) to obtain a microfiltration clear liquid; (4) concentrating; (5) sterilizing and filtering; and obtaining a polygonatum extract. It can be seen that the existing traditional Chinese medicine polygonatum effective ingredient extraction method lacks the determination of the processing end point, and it is difficult to ensure that the quality of products of different batches is the same, resulting in the difficulty in consistent quality of polygonatum and unstable active ingredients, which makes it difficult for polygonatum to be used as a medicine in a standardized manner. Summary of the invention

[0005] To this end, the present invention provides a method for preparing an adaptive polygonatum component extract, which is used to overcome the problem in the prior art that there is a lack of adaptive control in the polygonatum steaming process, it is difficult to ensure the maximum extraction of effective ingredients in polygonatum, and the quality of the polygonatum extract is low.

[0006] To achieve the above object, the present invention provides a method for preparing an adaptive polygonatum component extract, comprising:

[0007] Step S1, washing and steaming, taking fresh polygonatum, slicing and steaming for several times, monitoring each steaming process in real time to determine whether to adjust the steaming temperature, and collecting condensed liquid to obtain polygonatum extract;

[0008] Step S2, sampling and measuring the polygonatum sibiricum extract after each steaming, analyzing the quality of polysaccharides, and determining the steaming end point according to the change in the quality of polysaccharides;

[0009] The steaming end point is reached when the number of steaming times is six;

[0010] Step S3, when the steaming end point is reached, the polygonatum sibiricum extract is concentrated to obtain an extract;

[0011] Step S4, mixing the extract with the excipient in a preset ratio to obtain a soft material, sieving the soft material, and analyzing the sieving effect;

[0012] Step S5, determining whether to adjust the amount of excipient added or the single steaming time according to the screening effect.

[0013] Furthermore, real-time monitoring of each steaming process includes:

[0014] Analyze the apparent color change of polygonatum sibiricum in a preset monitoring period to obtain a real-time chromaticity difference, and calculate a real-time correlation coefficient according to the real-time chromaticity difference and the real-time condensation amount change rate;

[0015] The real-time correlation coefficient is judged according to the standard correlation coefficient, and whether the steaming process is abnormal is determined according to the judgment result;

[0016] When an abnormality occurs during the steaming process, the temperature distribution in the smart steamer is analyzed to determine whether the temperature in the smart steamer is uniform;

[0017] When it is determined that the temperature in the smart steamer is uneven, it is determined whether to adjust the opening of the exhaust valve in combination with the water level analysis result;

[0018] When it is determined that the temperature in the smart steamer is uniform, the steaming temperature is adjusted down.

[0019] Further, calculating the real-time correlation coefficient according to the real-time chromaticity difference and the real-time condensation amount change rate includes:

[0020] Obtain the real-time chromaticity value of the steamed polygonatum in the current monitoring period, calculate the percentage of the real-time chromaticity difference value and the standard chromaticity value, and obtain the color change percentage;

[0021] Obtain the volume change and initial volume of the polygonatum sibiricum extract in the current monitoring period, calculate the ratio of the volume change to the initial volume, and obtain the real-time condensation volume change rate;

[0022] The ratio of the color change percentage to the real-time condensation amount change rate is calculated to obtain the real-time correlation coefficient.

[0023] Further, determining whether the steaming process is abnormal according to the determination result includes:

[0024] When the real-time correlation coefficient is less than the standard correlation coefficient, it is determined that the steaming process is abnormal;

[0025] When the real-time correlation coefficient is greater than or equal to the standard correlation coefficient, it is determined that the steaming process is normal.

[0026] Further, analyzing the temperature distribution in the smart steamer to determine whether the temperature in the smart steamer is uniform includes:

[0027] Calculate the maximum temperature deviation value according to the corresponding real-time unit temperature in each monitoring area in the intelligent steamer;

[0028] Compare the standard temperature deviation value with the maximum temperature deviation value:

[0029] If the maximum temperature deviation value is less than or equal to the standard temperature deviation value, it is determined that the temperature in the smart steamer is uniform;

[0030] If the maximum temperature deviation value is greater than the standard temperature deviation value, it is determined that the temperature in the smart steamer is uneven.

[0031] Further, the maximum temperature deviation value is calculated according to the corresponding real-time unit temperature in each monitoring area in the intelligent steamer, including:

[0032] Obtain the real-time unit temperature corresponding to each monitoring area in the smart steamer;

[0033] Add the temperatures of each real-time unit and divide by the number of monitoring areas to get the average temperature;

[0034] Subtract the average temperature from each real-time unit temperature to obtain several unit temperature deviation values;

[0035] Take the absolute value of each unit temperature deviation value, obtain the value with the largest absolute value, and obtain the maximum temperature deviation value.

[0036] Further, determining whether to adjust the opening of the exhaust valve in combination with the water level analysis result includes:

[0037] Get the real-time water level in the steamer and compare it with the standard water level:

[0038] If the real-time water level is lower than the standard water level, the heating mode will be started and stopped;

[0039] If the real-time water level is greater than or equal to the standard water level, the initial opening of the exhaust valve is increased to a corrected opening;

[0040] Among them, the corrected opening is the product of the initial opening and the adjustment coefficient, and the adjustment coefficient is 1.2.

[0041] Furthermore, the excipient is hydroxypropyl methylcellulose or starch or lactose, and the preset ratio is extract: excipient = 1:2.

[0042] Furthermore, the screening process of the soft material includes:

[0043] The soft material is sieved through a 12-mesh screen to obtain particles, obtain the uniformity of the particles, and analyze whether the particle size meets the standard.

[0044] Further, determining whether to adjust the amount of excipient added or the single steaming time according to the sieving effect includes:

[0045] If the particles are uneven, screen out the particles that do not meet the standard particle size, and compare the particle size corresponding to each particle with the standard particle size:

[0046] If the particle sizes are all smaller than or equal to the standard particle size, adjust the amount of excipient added;

[0047] If the particle size of the particles is larger than the standard particle size, adjust the single steaming time.

[0048] Compared with the prior art, the beneficial effect of the present invention lies in that, since high temperature causes polysaccharides to degrade and destroy the carboxyl groups in the polysaccharides, thereby reducing the polysaccharide extraction rate, the apparent color change of the polygonatum after each steaming is monitored, and the number of steaming times is accurately monitored, so that the processing reaches the optimal state, and the invalid steaming time is shortened, the steaming end point is intelligently determined, the extraction efficiency is improved, and the product quality is guaranteed; the type of polygonatum selected is Yunnan polygonatum, and the fresh Yunnan polygonatum is washed to remove the soil, and the fibrous roots are removed before being steamed in an intelligent steamer. During each steaming process, the apparent color change of the polygonatum and the condensation amount are correlated and analyzed to determine whether to adjust the set parameters for the next steaming, so as to realize adaptive control, maximize the extraction of the effective ingredients in the polygonatum, optimize the production process, and ensure the quality and stability of the polygonatum extract.

[0049] Furthermore, by correlating the degree of color change of steamed Polygonatum sibiricum and the degree of change of condensation amount, it is determined whether the steaming process is normal, and the steaming process is intelligently monitored to ensure product quality and improve steaming efficiency. When it is determined that the steaming process is abnormal, it is analyzed whether the temperature distribution in the intelligent steamer is uniform, that is, whether the local temperature in the intelligent steamer is increased due to uneven temperature distribution, which destroys the structure of Polygonatum sibiricum polysaccharide, causes partial polysaccharide degradation, reduces the polysaccharide extraction rate, and thus reduces the condensation amount. The relationship between the color change of Polygonatum sibiricum and the change of condensation amount is weakened. By real-time monitoring of the steaming process and the status in the intelligent steamer, the safety and effectiveness of the extraction process are ensured.

[0050] Furthermore, since the change in steam flow in the steamer will lead to uneven temperature distribution in the steamer, causing the temperature in some areas to be too high, thereby causing the temperature in the steamer to rise suddenly, thereby destroying the structure of Polygonatum sibiricum polysaccharide, causing some polysaccharides to degrade and reducing the polysaccharide extraction rate, the smart steamer is divided into grids to form corresponding monitoring areas, and corresponding temperature sensors are set in each monitoring area to accurately analyze the temperature distribution in the smart steamer and improve monitoring accuracy.

[0051] Furthermore, the antioxidant activity of the samples was evaluated by converting their antioxidant capacity into a value equivalent to vitamin C (VC). BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 The figure is a schematic flow chart of a method for preparing an adaptive polygonatum component extract according to an embodiment of the present invention;

[0053] Figure 2 This is a schematic diagram of a process for real-time monitoring of each steaming process according to an embodiment of the present invention;

[0054] Figure 3 A schematic diagram of a flow chart of calculating a real-time correlation coefficient according to an embodiment of the present invention;

[0055] Figure 4 The figure is a schematic diagram of a process for determining whether the temperature in the smart steamer is uniform according to an embodiment of the present invention. DETAILED DESCRIPTION

[0056] In order to make the objects and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0057] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the protection scope of the present invention.

[0058] It should be noted that, in the description of the present invention, terms such as "up", "down", "left", "right", "inside" and "outside" indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings. This is merely for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.

[0059] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0060] See also Figure 1 As shown, it is a schematic flow chart of a method for preparing an adaptive polygonatum component extract according to an embodiment of the present invention. The present invention provides a method for preparing an adaptive polygonatum component extract, comprising:

[0061] Step S1, washing and steaming, taking fresh polygonatum, slicing and steaming for several times, monitoring each steaming process in real time to determine whether to adjust the steaming temperature, and collecting condensed liquid to obtain polygonatum extract;

[0062] Step S2, sampling and measuring the polygonatum sibiricum extract after each steaming, analyzing the quality of polysaccharides, and determining the steaming end point according to the change in the quality of polysaccharides;

[0063] The steaming end point is reached when the number of steaming times is six;

[0064] Step S3, when the steaming end point is reached, the polygonatum sibiricum extract is concentrated to obtain an extract;

[0065] Step S4, mixing the extract with the excipient in a preset ratio to obtain a soft material, sieving the soft material, and analyzing the sieving effect;

[0066] Step S5, determining whether to adjust the amount of excipient added or the single steaming time according to the screening effect.

[0067] In this embodiment, high-temperature steam processing is used to process polygonatum. Since high temperature causes polysaccharides to degrade and destroy the carboxyl groups in the polysaccharides, thereby reducing the polysaccharide extraction rate, the apparent color change of polygonatum after each steaming is monitored, and the number of steaming times is accurately monitored to achieve the optimal processing state, shorten the invalid steaming time, and intelligently determine the steaming end point, thereby improving the extraction efficiency and ensuring the product quality. The selected polygonatum species is Yunnan polygonatum, and the fresh Yunnan polygonatum is washed to remove the soil, and the fibrous roots are removed before being steamed in an intelligent steamer. During each steaming process, the apparent color change of polygonatum and the condensation amount are correlated and analyzed to determine whether to adjust the setting parameters for the next steaming, thereby realizing adaptive control, maximizing the extraction of effective ingredients in polygonatum, and optimizing The production process ensures the quality and stability of the polygonatum extract; the total steaming time reflects the dynamic changes in the antioxidant activity of the polysaccharide, that is, the antioxidant activity of the polygonatum polysaccharide first increases and then decreases. This is because the optimization of the molecular structure of the polysaccharide in the early stage of processing enhances its antioxidant properties, while excessive processing leads to the destruction of active sites or structural degradation, thereby reducing the antioxidant capacity. Therefore, the condensate after each steaming is sampled and measured, and the polysaccharide quality is analyzed. The steaming end point is determined according to the change in polysaccharide quality, and the polysaccharide quality is characterized by the measured antioxidant activity of the polysaccharide; the number of steaming is 6 times, at which time the inside of the polygonatum appears black, the weight no longer increases, and the polysaccharide antioxidant activity is the highest; after each steaming, it is ventilated and dried outdoors for 1 day before continuing the next steaming.

[0068] See also Figure 2 As shown, it is a schematic diagram of a process of real-time monitoring of each steaming process according to an embodiment of the present invention;

[0069] Specifically, real-time monitoring of each steaming process includes:

[0070] Step S1001, analyzing the apparent color change of polygonatum sibiricum in a preset monitoring period to obtain a real-time chromaticity difference, and calculating a real-time correlation coefficient according to the real-time chromaticity difference and the real-time condensation amount change rate;

[0071] Step S1002, judging the real-time correlation coefficient according to the standard correlation coefficient, and determining whether the steaming process is abnormal according to the judgment result;

[0072] Step S1003, when an abnormality occurs during the steaming process, analyzing the temperature distribution in the smart steamer to determine whether the temperature in the smart steamer is uniform;

[0073] Step S1004, when it is determined that the temperature in the smart steamer is uneven, determine whether to adjust the opening of the exhaust valve in combination with the water level analysis result;

[0074] When it is determined that the temperature in the smart steamer is uniform, the steaming temperature is adjusted down to the corrected steaming temperature;

[0075] The corrected steaming temperature is the difference between the steaming temperature and the first correction step, and the first correction step is 5°C.

[0076] The real-time chromaticity difference in the present embodiment indicates the amount of change in the apparent color of Polygonatum. As the steaming time of Polygonatum increases, the color difference between steamed Polygonatum and raw Polygonatum becomes greater and greater, and the amount of condensation becomes greater and greater. Therefore, by associating the degree of color change of steamed Polygonatum and the degree of change of condensation, it is possible to determine whether the steaming process is normal, and intelligently monitor the steaming process to ensure product quality and improve steaming efficiency. When it is determined that the steaming process is abnormal, it is analyzed whether the temperature distribution in the intelligent steamer is uniform, that is, whether the local temperature in the intelligent steamer is increased due to uneven temperature distribution, thereby destroying the structure of Polygonatum polysaccharide, causing partial degradation of polysaccharides, and reducing the polysaccharide extraction rate, thereby reducing the condensation amount, and weakening the relationship between the color change of Polygonatum and the change in condensation amount. By real-time monitoring of the steaming process and the state in the intelligent steamer, the safety and effectiveness of the extraction process are ensured.

[0077] See also Figure 3 As shown, it is a schematic diagram of the process of calculating the real-time correlation coefficient according to an embodiment of the present invention;

[0078] Specifically, the real-time correlation coefficient is calculated based on the real-time chromaticity difference and the real-time condensation amount change rate, including:

[0079] Step S1011, obtaining the real-time chromaticity value of the steamed polygonatum in the current monitoring period, calculating the percentage of the real-time chromaticity difference value and the standard chromaticity value, and obtaining the color change percentage;

[0080] Step S1021, obtaining the volume change and initial volume of the polygonatum sibiricum extract in the current monitoring period, calculating the ratio of the volume change to the initial volume, and obtaining the real-time condensation volume change rate;

[0081] Step S1031, calculating the ratio of the color change percentage to the real-time condensation amount change rate to obtain a real-time correlation coefficient.

[0082] The calculation of the initial chromaticity value of the steamed polygonatum in this embodiment is to measure the chromaticity of the sample powder by a colorimeter. In each monitoring period, the steamed polygonatum is sampled, crushed, and sieved. The sieving is to ensure that the particles of the powder are uniform, thereby ensuring the accuracy of the measurement. The taken powder is placed under the colorimeter for measurement, and each sample powder is flattened, and the measurement position is randomly changed under the colorimeter to ensure that the data at different positions are representative. After each sample is flattened, 6 images are taken in parallel to avoid measurement errors caused by local factors (such as the distribution of particles). A standard colorimetric plate is used for correction. The function of the standard colorimetric plate is to calibrate the colorimeter to ensure the measurement accuracy. The benchmark is accurate. For each sample, record the three chromaticity parameters of brightness value (L*), red-green phase (a*) and yellow-blue phase (b*). L* represents the brightness of the sample. The larger the value, the higher the brightness. The range of L* is usually 0 (black) to 100 (white). a* represents the red-green preference of the sample. The larger the value, the redder the color. The smaller the value, the greener the color. Usually, the range of a* is negative to positive, with negative values ​​tending to green and positive values ​​tending to red. b*: represents the yellow-blue preference of the sample. The larger the value, the yellower the color. The smaller the value, the bluer the color. The range of b* is usually negative to positive, with negative values ​​tending to blue and positive values ​​tending to yellow. Calculate the total color value (E*) of each sample. ab ) as the real-time color difference value, which indicates the color difference between the sample and the standard sample, E* ab =(a* 2 +b* 2 +L* 2 ) 1 / 2 , the calculated E* ab The larger the value, the greater the difference between the sample color and the standard color; E* ab The smaller the value, the smaller the color difference.

[0083] Specifically, determining whether the steaming process is abnormal according to the determination result includes:

[0084] When the real-time correlation coefficient is less than the standard correlation coefficient, it is determined that the steaming process is abnormal;

[0085] When the real-time correlation coefficient is greater than or equal to the standard correlation coefficient, it is determined that the steaming process is normal.

[0086] The standard correlation coefficient in this embodiment is a threshold value set for judging the quality of the extraction effect. The standard correlation coefficient is set to 0.5, and can be adjusted according to the actual type of raw materials. The standard correlation coefficient is pre-measured in the experiment. 50 g of Polygonatum odoratum is taken and processed at a steaming temperature of 105°C for two hours. During the processing, the real-time correlation coefficient is calculated every 10 minutes until the end, and the minimum value of the real-time correlation coefficient is taken as the standard correlation coefficient.

[0087] In this embodiment, the chromaticity value of Polygonatum sibiricum and the volume of condensate during the steaming process are recorded in each monitoring cycle to analyze the relationship between the apparent color change and the change in condensate amount, that is, the real-time correlation coefficient is calculated to analyze the relationship between the apparent color change and the change in condensate amount. The higher the value, the stronger the relationship between the color change and the change in condensate amount, and the better the extraction effect. For example, in any monitoring cycle, the color change of Polygonatum sibiricum changes from the initial 80 to 100, and the color change percentage is (100-80) / 100=20%, the volume of condensate changes from 200 ml to 150 ml, and the condensate change percentage is (200-150) / 200=25%, and the real-time correlation coefficient is 20% / 25%=0.8. Since 0.8 is higher than the preset value of 0.5, it means that the extraction effect is good and the current steaming parameters can be continued.

[0088] By setting the standard correlation coefficient, the steaming extraction process of Polygonatum sibiricum polysaccharide can be effectively monitored and optimized to ensure that the extracted active ingredients meet the expected quality standards and that the quality of different batches of products is consistent.

[0089] See also Figure 4 As shown, it is a schematic diagram of a process of determining whether the temperature in the smart steamer is uniform according to an embodiment of the present invention;

[0090] Specifically, analyzing the temperature distribution in the smart steamer to determine whether the temperature in the smart steamer is uniform includes:

[0091] Step S1013, calculating the maximum temperature deviation value according to the corresponding real-time unit temperature in each monitoring area in the intelligent steamer;

[0092] Step S1023, comparing the standard temperature deviation value with the maximum temperature deviation value, and determining whether the temperature in the smart steamer is uniform according to the comparison result;

[0093] If the maximum temperature deviation value is less than or equal to the standard temperature deviation value, it is determined that the temperature in the smart steamer is uniform;

[0094] If the maximum temperature deviation value is greater than the standard temperature deviation value, it is determined that the temperature in the smart steamer is uneven.

[0095] The standard temperature deviation value set in this embodiment represents the threshold value for allowing temperature fluctuations. The standard temperature deviation value is set between 3% and 5% of the steaming temperature. When the maximum temperature deviation value is less than or equal to the standard temperature deviation value, it indicates that the temperature distribution in the steamer is uniform. In this case, the set steaming temperature is reduced to avoid high temperature causing degradation of polysaccharides and destroying the carboxyl groups in the polysaccharides, thereby reducing the polysaccharide extraction rate.

[0096] Specifically, the maximum temperature deviation value is calculated according to the corresponding real-time unit temperature in each monitoring area in the smart steamer, including:

[0097] Obtain the real-time unit temperature corresponding to each monitoring area in the smart steamer;

[0098] Add the temperatures of each real-time unit and divide by the number of monitoring areas to get the average temperature;

[0099] Subtract the average temperature from each real-time unit temperature to obtain several unit temperature deviation values;

[0100] Take the absolute value of each unit temperature deviation value, obtain the value with the largest absolute value, and obtain the maximum temperature deviation value.

[0101] Since the change of steam flow in the steamer will lead to uneven temperature distribution in the steamer and make the temperature in some areas too high, the temperature in the steamer will suddenly rise, which will destroy the structure of Polygonatum sibiricum polysaccharide, degrade some polysaccharides and reduce the polysaccharide extraction rate. Therefore, the smart steamer is divided into grids to form corresponding monitoring areas, and corresponding temperature sensors are set in each monitoring area to accurately analyze the temperature distribution in the smart steamer and improve the monitoring accuracy.

[0102] Specifically, determining whether to adjust the opening of the exhaust valve in combination with the water level analysis results includes:

[0103] Get the real-time water level in the steamer and compare it with the standard water level:

[0104] If the real-time water level is lower than the standard water level, the heating mode will be started and stopped;

[0105] If the real-time water level is greater than or equal to the standard water level, the initial opening of the exhaust valve is increased to a corrected opening;

[0106] Among them, the corrected opening is the product of the initial opening and the adjustment coefficient, and the adjustment coefficient is 1.2.

[0107] In this embodiment, the initial opening is 0.5 and the standard water level height is 2 / 3.

[0108] By monitoring the temperature distribution in the steamer and analyzing whether the steam flow is uniform, if the real-time water level is determined to be lower than the standard water level, it means that the water level in the steamer is too low, resulting in insufficient steam generation, uneven steam flow, and affecting the steaming effect. In this way, the heating mode will be started and stopped, and an alarm will be issued to enable relevant staff to check and handle the situation, thereby improving the intelligence of the steaming process. By increasing the opening of the exhaust valve, the uniformity of the steam flow can be effectively improved, the stability of the steaming process can be improved, and temperature fluctuations can be reduced, thereby increasing the extraction rate of Polygonatum sibiricum polysaccharide.

[0109] Specifically, the excipient is hydroxypropyl methylcellulose or starch or lactose, and the preset ratio is extract: excipient = 1:2.

[0110] Specifically, the screening process of the soft material includes:

[0111] The soft material is sieved through a 12-mesh screen to obtain particles, obtain the uniformity of the particles, and analyze whether the particle size meets the standard.

[0112] In this embodiment, a vibrating screen is used to screen the soft material, and the screening time is 2-5 minutes. The laser particle size analyzer is used to detect whether the particle size distribution of the soft material is uniform. If the particles are found to be uneven or the particle size does not meet the requirements during the screening process, the proportion of the excipient is appropriately adjusted. If it is found that the soft material particles contain unqualified coarse particles, the steaming setting parameters, that is, the single steaming time, are adjusted to improve the particle size of the extract.

[0113] Specifically, whether to adjust the amount of excipients or the duration of single steaming according to the sieving effect includes:

[0114] If the particles are uneven, screen out the particles that do not meet the standard particle size, and compare the particle size corresponding to each particle with the standard particle size:

[0115] If the particle sizes are all smaller than or equal to the standard particle size, adjust the amount of excipient added;

[0116] If there are particles with a diameter larger than the standard diameter, the single steaming time is adjusted to the corrected steaming time;

[0117] Among them, adjusting the amount of excipient added includes increasing the amount of excipient added by adjusting the preset ratio to 1:3; correcting the steaming time to be the sum of a single steaming time and a second correction step, and the second correction step is 5 minutes.

[0118] The single steaming time in this embodiment is the initial single steaming setting time, which is 60 minutes; the standard particle size is 100 mesh. When it is found that some particles are larger than 100 mesh during the steaming process, the steaming time is extended to promote the release of more effective ingredients, thereby making the particles of the extract finer, promoting a more uniform release of the ingredients of the extract, reducing the phenomenon of excessively large particles, optimizing the production process, and improving the quality of the polygonatum component extract and the uniformity of the particles.

[0119] Specifically, the condensate after each steaming is sampled and measured to analyze the polysaccharide quality. The steaming end point is determined based on the change in polysaccharide quality, including:

[0120] The antioxidant activity of the polysaccharide is obtained in a preset collection period, an activity change curve is generated, and the activity change curve is judged.

[0121] If a peak appears in the activity change curve, it is determined that the steaming process is completed and the steaming end point is obtained;

[0122] If no peak appears in the activity change curve, continue with the next steaming.

[0123] In this example, the antioxidant activity of polysaccharides was evaluated by calculating the VC equivalent antioxidant capacity, including the preparation of FRAP working solution, the drawing of standard curve, the FRAP measurement of samples, and the calculation of VC equivalent antioxidant capacity (VCEAC).

[0124] Preparation of FRAP working solution: 300mmol / L sodium acetate buffer solution (pH 3.6), 10mmol / L TPTZ solution (2,4,6-tripyridyl-s-triazine), and 20mmol / L ferric chloride solution (FeCl3). Mix the above three solutions in a volume ratio of 1:1:10 to obtain FRAP working solution.

[0125] Draw a standard curve: Prepare different concentrations of VC standard working solution (VC is vitamin C). Take 0.1mL of each VC standard working solution and mix them with the FRAP working solution. Leave them to react for 30 minutes at room temperature in the dark. Measure the absorbance (A value) of each mixed solution at 593nm, and repeat the experiment three times. According to the linear relationship between VC concentration and A value, regression analysis is performed to obtain the linear regression equation: Y = 0.1612X + 0.100, R 2 =0.9987;

[0126] Wherein, Y represents the measured absorbance (A value), and X represents the concentration of VC (unit: μmol / L). The linear range is between 142 and 994 μmol / L.

[0127] Sample FRAP assay: Take 0.1 mL of each test solution and mix it thoroughly with 3.9 mL of FRAP working solution. Avoid light and oscillate for 30 minutes. Use 95% ethanol as a blank control, measure the absorbance (A value) of the sample at 593 nm, and repeat the experiment three times.

[0128] Calculation of VC equivalent antioxidant capacity (VCEAC):

[0129] According to the DPPH, ABTS+· free radical scavenging rate and A value in the FRAP assay results, the VC equivalent antioxidant capacity (VCEAC) of the sample was calculated using the following formula: X = [(Ab) / (a×m)];

[0130] Wherein, X represents the VC equivalent antioxidant capacity of the sample (unit: mmol / mg); A represents the free radical scavenging ability of the sample or the absorbance of the sample in the FRAP assay (A value); b represents the intercept of each standard curve; a represents the slope of each standard curve; and m represents the sample mass (unit: mg).

[0131] The antioxidant activity of the samples was evaluated by converting their antioxidant capacity into a value equivalent to vitamin C (VC).

[0132] Specifically, the preparation method of this embodiment was adopted, and the total sugar content of Polygonatum sibiricum was measured as group 6-6 after steaming 6 times, and compared with the control group, 100℃ group, 110℃ group, and 4-4 group, respectively; the steaming temperature of the 100℃ group was fixed at 100℃, the steaming temperature of the 110℃ group was fixed at 110℃, and the steaming times of the 4-4 group were fixed at 4 times.

[0133] The experimental results are shown in Table 1, wherein Table 1 is a comparison of the steamed polygonatum indexes and results of the embodiments of the present invention.

[0134] Table 1

[0135] Group Total sugar content (mg / g) Control group 52.47±3.81 100℃ Group 82.76±5.95 130℃ Group 32.05±3.85 4-4 groups 64.26±5.17 Group 6-6 83.52±6.31

[0136] The adaptive method used in this embodiment avoids excessive steaming through real-time monitoring of the process, which results in the hydrolysis of polysaccharides and part of carbohydrates under high temperature to form oligosaccharides and monosaccharides, and the Maillard reaction of monosaccharides with amino acids, thereby reducing the total sugar content. Experimental data are provided to confirm that the above preparation method can maximize the extraction of Polygonatum sibiricum polysaccharides.

[0137] So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

[0138] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing an adaptive polygonatum component extract, characterized in that: include, Step S1, washing and steaming, taking fresh polygonatum, slicing and steaming for several times, monitoring each steaming process in real time to determine whether to adjust the steaming temperature, and collecting condensed liquid to obtain polygonatum extract; Step S2, sampling and measuring the polygonatum sibiricum extract after each steaming, analyzing the quality of polysaccharides, and determining the steaming end point according to the change in the quality of polysaccharides; The steaming end point is reached when the number of steaming times is six; Step S3, when the steaming end point is reached, the polygonatum sibiricum extract is concentrated to obtain an extract; Step S4, mixing the extract with the excipient in a preset ratio to obtain a soft material, sieving the soft material, and analyzing the sieving effect; Step S5, determining whether to adjust the amount of excipient added or the single steaming time according to the screening effect.

2. The method for preparing the adaptive polygonatum component extract according to claim 1, characterized in that: Real-time monitoring of each steaming process includes: Analyze the apparent color change of polygonatum sibiricum in a preset monitoring period to obtain a real-time chromaticity difference, and calculate a real-time correlation coefficient according to the real-time chromaticity difference and the real-time condensation amount change rate; The real-time correlation coefficient is judged according to the standard correlation coefficient, and whether the steaming process is abnormal is determined according to the judgment result; When an abnormality occurs during the steaming process, the temperature distribution in the smart steamer is analyzed to determine whether the temperature in the smart steamer is uniform; When it is determined that the temperature in the smart steamer is uneven, it is determined whether to adjust the opening of the exhaust valve in combination with the water level analysis result; When it is determined that the temperature in the smart steamer is uniform, the steaming temperature is adjusted down.

3. The method for preparing the adaptive polygonatum component extract according to claim 2, characterized in that: The real-time correlation coefficient is calculated based on the real-time chromaticity difference and the real-time condensation amount change rate, including: Obtain the real-time chromaticity value of the steamed polygonatum in the current monitoring period, calculate the percentage of the real-time chromaticity difference value and the standard chromaticity value, and obtain the color change percentage; Obtain the volume change and initial volume of the polygonatum sibiricum extract in the current monitoring period, calculate the ratio of the volume change to the initial volume, and obtain the real-time condensation volume change rate; The ratio of the color change percentage to the real-time condensation amount change rate is calculated to obtain the real-time correlation coefficient.

4. The method for preparing the adaptive polygonatum component extract according to claim 2, characterized in that: Determine whether the steaming process is abnormal based on the judgment results, including: When the real-time correlation coefficient is less than the standard correlation coefficient, it is determined that the steaming process is abnormal; When the real-time correlation coefficient is greater than or equal to the standard correlation coefficient, it is determined that the steaming process is normal.

5. The method for preparing the adaptive polygonatum component extract according to claim 2, characterized in that: Analyze the temperature distribution in the smart steamer to determine whether the temperature in the smart steamer is uniform, including: Calculate the maximum temperature deviation value according to the corresponding real-time unit temperature in each monitoring area in the intelligent steamer; Compare the standard temperature deviation value with the maximum temperature deviation value: If the maximum temperature deviation value is less than or equal to the standard temperature deviation value, it is determined that the temperature in the smart steamer is uniform; If the maximum temperature deviation value is greater than the standard temperature deviation value, it is determined that the temperature in the smart steamer is uneven.

6. The method for preparing the adaptive polygonatum component extract according to claim 5, characterized in that: The maximum temperature deviation value calculated based on the corresponding real-time unit temperature in each monitoring area in the smart steamer includes: Obtain the real-time unit temperature corresponding to each monitoring area in the smart steamer; Add the temperatures of each real-time unit and divide by the number of monitoring areas to get the average temperature; Subtract the average temperature from each real-time unit temperature to obtain several unit temperature deviation values; Take the absolute value of each unit temperature deviation value, obtain the value with the largest absolute value, and obtain the maximum temperature deviation value.

7. The method for preparing the adaptive polygonatum component extract according to claim 2, characterized in that: Determine whether to adjust the opening of the exhaust valve in combination with the water level analysis results, including: Get the real-time water level in the steamer and compare it with the standard water level: If the real-time water level is lower than the standard water level, the heating mode will be started and stopped; If the real-time water level is greater than or equal to the standard water level, the initial opening of the exhaust valve is increased to a corrected opening; Among them, the corrected opening is the product of the initial opening and the adjustment coefficient, and the adjustment coefficient is 1.

2.

8. The method for preparing the adaptive polygonatum component extract according to claim 1, characterized in that: The excipient is hydroxypropyl methylcellulose or starch or lactose, and the preset ratio is extract: excipient = 1:

2.

9. The method for preparing the adaptive polygonatum component extract according to claim 1, characterized in that: The screening process for the soft material comprises: The soft material is sieved through a 12-mesh screen to obtain particles, obtain the uniformity of the particles, and analyze whether the particle size meets the standard.

10. The method for preparing the adaptive polygonatum component extract according to claim 1, characterized in that: Determine whether to adjust the amount of excipients added or the single steaming time according to the sieving effect, including: If the particles are uneven, screen out the particles that do not meet the standard particle size, and compare the particle size corresponding to each particle with the standard particle size: If the particle sizes are all smaller than or equal to the standard particle size, adjust the amount of excipient added; If the particle size of the particles is larger than the standard particle size, adjust the single steaming time.

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