Multi-wavelength non-phase line beam generation device, generation method and line scanning imaging equipment
Through the multi-wavelength non-coherent line beam generation device, the LED light source of multiple colors combines the beam to generate the line beam, which solves the problem of poor speckle noise and color reduction in laser imaging, and achieves high power density and true color imaging effects.
Patent Information
- Application Number
- CN202311485181.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-11-09
- Publication Date
- 2025-05-09
AI Technical Summary
In line scanning imaging, the coherence of the laser leads to speckle noise, and the narrow spectral bandwidth leads to poor color reduction, making true color images unable to be obtained.
A multi-wavelength non-coherent beam generation device, including an LED light source module and a control device, is used to generate an incoherent beam with a high power density and multi-wavelength component through the beam-combination of three different colors of LED light sources (blue, green, and red).
The generated line beam has a high power density and multi-wavelength component, avoiding speckle noise, and obtaining true color sample images, which meets the requirements of high power density.
Smart Images
Figure CN119960158A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of line scanning imaging, and in particular to a multi-wavelength incoherent linear light beam generating device, a generating method and a line scanning imaging device. Background Art
[0002] Line scanning imaging, such as laser line scanning confocal microscopy, requires the generation of a thinner line beam with a higher power density. Generally, a cylindrical mirror is introduced after the laser beam is collimated to generate a thinner line beam with a higher power density. There are two problems with using line beam laser for scanning imaging: first, the laser has strong coherence, which will cause laser speckle noise during imaging; second, the spectral bandwidth of the laser is very narrow, and the color reproduction during imaging is poor, so it is impossible to obtain a true color image of the sample.
[0003] LED light source is a kind of light source with high brightness and incoherent nature. It is a potential light source for generating high-power density line beams. LED light source has a large divergence angle. Simply adding a slit in front of the LED light source to generate a line beam effectively utilizes very little light energy. The light power density of the generated line beam is small. When used for line scanning confocal imaging, the light intensity is weak and the image signal-to-noise ratio is poor. Summary of the invention
[0004] In view of this, it is necessary to provide a multi-wavelength incoherent linear light beam generating device, generating method and line scanning imaging equipment to address the defect of poor image signal-to-noise ratio in the prior art line scanning imaging.
[0005] To solve the above problems, this application adopts the following technical solutions:
[0006] One of the purposes of the present application is to provide a multi-wavelength incoherent linear light beam generating device, comprising: an LED light source module and a control device, wherein:
[0007] The LED light source module comprises: a first LED light source unit, a second LED light source unit and a third LED light source unit, wherein the first LED light source unit comprises a first LED light source, a first condenser, a first slit aperture and a first dichroic mirror; the second LED light source unit comprises a second LED light source, a second condenser, a second dichroic mirror and a second slit aperture; the third LED light source unit comprises a third LED light source and a third condenser; a light beam emitted by the first LED light source is irradiated on the first slit aperture after passing through the first condenser, a line light beam emitted from the first slit aperture is incident on the first dichroic mirror and is reflected by the first dichroic mirror; a light beam emitted by the second LED light source is reflected by the second dichroic mirror after passing through the second condenser and is irradiated on the second slit aperture; a light beam emitted by the third LED light source is transmitted by the second dichroic mirror after passing through the third condenser and is irradiated on the second slit aperture, and light beams of the second LED light source and the third LED light source emitted from the second slit aperture are incident on the first dichroic mirror and are transmitted by the first dichroic mirror;
[0008] The control module is electrically connected to the first LED light source unit, the second LED light source unit and the third LED light source unit, and is used to control the power and switching of the first LED light source, the second LED light source and the third LED light source.
[0009] In some embodiments, the first LED light source, the second LED light source, and the third LED light source are light sources of different colors.
[0010] In some embodiments, the first LED light source is blue, the second LED light source is green, and the third LED light source is red.
[0011] In some of the embodiments, when the first LED light source, the second LED light source and the third LED light source are turned on at full power at the same time, a white light beam can be generated; when the first LED light source, the second LED light source and the third LED light source are turned on individually, a monochrome line beam can be generated; when the first LED light source, the second LED light source and the third LED light source are all turned on at a certain ratio of light power, a line beam of a specific color can be generated.
[0012] In some of the embodiments, the slit widths of the first slit aperture and the second slit aperture can be set to different widths to generate line light beams of different widths.
[0013] In some embodiments, the first condenser, the second condenser and the third condenser are all collimators, and the LED light source is collimated by the corresponding collimator and then irradiated onto the corresponding slit aperture.
[0014] In some embodiments, the first condenser, the second condenser and the third condenser are all in the form of a combination of a collimator and a cylindrical lens, and the LED light source is collimated by the corresponding collimator and focused by the corresponding cylindrical lens to irradiate the corresponding slit aperture.
[0015] The second object of the present application is to provide a method for generating a multi-wavelength incoherent linear light beam generating device, comprising the following steps:
[0016] The control module controls the power and switching of the first LED light source, the second LED light source and the third LED light source;
[0017] The light beam emitted by the first LED light source passes through the first condenser and is irradiated on the first slit diaphragm, and the line light beam emitted from the first slit diaphragm is incident on the first dichroic mirror and reflected by the first dichroic mirror; the light beam emitted by the second LED light source passes through the second condenser and is reflected by the second dichroic mirror and is irradiated on the second slit diaphragm; the light beam emitted by the third LED light source passes through the third condenser and is transmitted by the second dichroic mirror and is irradiated on the second slit diaphragm, and the light beams of the second LED light source and the third LED light source emitted from the second slit diaphragm are incident on the first dichroic mirror and are transmitted by the first dichroic mirror.
[0018] The third object of the present application is to provide a line scanning imaging device, including the multi-wavelength incoherent linear light beam generating device.
[0019] This application adopts the above technical solution, and its beneficial effects are as follows:
[0020] The present application provides a multi-wavelength incoherent line light beam generating device, generating method and line scanning imaging device, wherein the control module controls the power and switch of the first LED light source, the second LED light source and the third LED light source, the light beam emitted by the first LED light source passes through the first condenser and is irradiated on the first slit diaphragm, the line light beam emitted from the first slit diaphragm is incident on the first dichroic mirror and reflected by the first dichroic mirror; the light beam emitted by the second LED light source passes through the second condenser and is reflected by the second dichroic mirror and then irradiated on the second slit diaphragm; the light beam emitted by the third LED light source passes through the third condenser and is transmitted by the second dichroic mirror and then irradiated on the second slit diaphragm, the light beams of the second LED light source and the third LED light source emitted from the second slit diaphragm are incident on the first dichroic mirror and transmitted by the first dichroic mirror, the present application uses LED as the light source for generating the line light beam, the LED has incoherence, there is no speckle noise, and a thin incoherent line light beam with a higher power density can be generated, and the light beam has multiple wavelength components, and when the generated line light beam is used for scanning imaging, there is no speckle noise in the image.
[0021] In addition, the line light beam generated by the present application has a higher power density or brightness. By using three high-power LED light sources of different colors and combining the light beams, the generated line light beam has a higher power density than directly generating a line light beam using a white light LED alone. This can better meet the requirements of line scanning confocal imaging with higher power density requirements.
[0022] In addition, the line light beam generated by the present application can obtain a true color sample image when used for line scanning imaging. The LED light source has a wider spectral width than the laser. The line light beam generated by combining red, green and blue LED light sources can be used for line scanning imaging to obtain a true color image of the sample. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments of the present application or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0024] Figure 1 A schematic diagram of the structure of a multi-wavelength incoherent linear light beam generating device provided in one embodiment of the present application.
[0025] Figure 2 A schematic structural diagram of a multi-wavelength incoherent linear light beam generating device provided in another embodiment of the present application. DETAILED DESCRIPTION
[0026] Embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present application, and should not be construed as limiting the present application.
[0027] In the description of the present application, it should be understood that the terms "upper", "lower", "horizontal", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.
[0028] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.
[0029] In order to make the objectives, technical solutions and advantages of the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments.
[0030] See also Figure 1 The multi-wavelength incoherent linear light beam generating device provided in the embodiment of the present application includes: an LED light source module 10 and a control device 20. The specific implementation of each component is described in detail below.
[0031] The LED light source module 10 includes a first LED light source unit 110 , a second LED light source unit 120 and a third LED light source unit 130 .
[0032] The first LED light source unit 110 includes a first LED light source 111 , a first condenser 112 , a first slit aperture 113 and a first dichroic mirror 114 .
[0033] The second LED light source unit 120 includes a second LED light source 121 , a second condenser 122 , a second dichroic mirror 123 , and a second slit aperture 124 .
[0034] The third LED light source unit 130 includes a third LED light source 131 and a third condenser lens 132 .
[0035] The control module 20 is electrically connected to the first LED light source unit 110 , the second LED light source unit 120 and the third LED light source unit 130 , and is used to control the power and switching of the first LED light source 111 , the second LED light source 121 and the third LED light source 131 .
[0036] The multi-wavelength incoherent linear light beam generating device provided in the present application works as follows:
[0037] The light beam emitted by the first LED light source 111 passes through the first condenser 112 and is irradiated on the first slit aperture 113. The line light beam emitted from the first slit aperture 113 is incident on the first dichroic mirror 114 and is reflected by the first dichroic mirror 114. The light beam emitted by the second LED light source 121 passes through the second condenser 122 and is reflected by the second dichroic mirror 122 and is irradiated on the second slit aperture 124. The light beam emitted by the third LED light source 131 passes through the third condenser 132 and is transmitted by the second dichroic mirror 123 and is irradiated on the second slit aperture 124. The light beams of the second LED light source 121 and the third LED light source 131 emitted from the second slit aperture 124 are incident on the first dichroic mirror 114 and are transmitted by the first dichroic mirror 114.
[0038] In some embodiments, the first LED light source 111 , the second LED light source 121 , and the third LED light source 131 are light sources of different colors.
[0039] Furthermore, the first LED light source 111 is blue, the second LED light source 121 is green, and the third LED light source 131 is red.
[0040] It can be understood that LED light sources have a wider spectrum than lasers. When the line light beam generated by combining red, green and blue LED light sources is used for line scanning imaging, a true color image of the sample can be obtained.
[0041] Furthermore, when the first LED light source 111, the second LED light source 121 and the third LED light source 131 are turned on at full power at the same time, a white light beam can be generated; when the first LED light source 111, the second LED light source 121 and the third LED light source 131 are turned on separately, a monochrome line beam can be generated; when the first LED light source 111, the second LED light source 121 and the third LED light source 131 are all turned on at a certain proportion of light power, a line beam of a specific color can be generated.
[0042] It can be understood that the line light beam generated in this embodiment has a higher power density or brightness. By using three high-power LED light sources of different colors and combining the light beams, the generated line light beam has a higher power density than directly generating a line light beam using a white light LED alone. This can better meet the requirements of line scanning confocal imaging with higher power density requirements.
[0043] Furthermore, the slit widths of the first slit aperture 113 and the second slit aperture 124 may be set to different widths to generate line light beams of different widths.
[0044] Furthermore, the first condenser 112, the second condenser 122 and the third condenser 132 are all collimators, and the LED light source is collimated by the corresponding collimator and then irradiated onto the corresponding slit diaphragm.
[0045] See also Figure 2 The first condenser 112, the second condenser 122 and the third condenser 132 are all in the form of a combination of a collimator and a cylindrical lens (115, 125, 123 in the figure), and the LED light source is collimated by the corresponding collimator and focused by the corresponding cylindrical lens and then irradiated on the corresponding slit aperture.
[0046] The multi-wavelength incoherent line light beam generating device and generating method provided in the above-mentioned embodiments of the present application adopt LED as the light source for generating the line light beam. The LED is incoherent and does not have speckle noise. It can generate a relatively thin incoherent line light beam with a higher power density. At the same time, the light beam has multi-wavelength components. When the generated line light beam is used for scanning imaging, there is no speckle noise in the image.
[0047] It can be understood that the technical features of the above-described embodiments can be arbitrarily combined. In order to make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0048] The above are only preferred embodiments of the present application, and only specifically describe the technical principles of the present application. These descriptions are only for explaining the principles of the present application and cannot be interpreted as limiting the scope of protection of the present application in any way. Based on the explanation here, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application, and other specific implementation methods of the present application that can be associated with the technicians in this field without creative work, should be included in the scope of protection of the present application.
Claims
1. A multi-wavelength incoherent linear beam generating device, characterized in that: include: LED light source module and control device, wherein: The LED light source module comprises: a first LED light source unit, a second LED light source unit and a third LED light source unit, wherein the first LED light source unit comprises a first LED light source, a first condenser, a first slit aperture and a first dichroic mirror; the second LED light source unit comprises a second LED light source, a second condenser, a second dichroic mirror and a second slit aperture; the third LED light source unit comprises a third LED light source and a third condenser; a light beam emitted by the first LED light source is irradiated on the first slit aperture after passing through the first condenser, a line light beam emitted from the first slit aperture is incident on the first dichroic mirror and is reflected by the first dichroic mirror; a light beam emitted by the second LED light source is reflected by the second dichroic mirror after passing through the second condenser and is irradiated on the second slit aperture; a light beam emitted by the third LED light source is transmitted by the second dichroic mirror after passing through the third condenser and is irradiated on the second slit aperture, and light beams of the second LED light source and the third LED light source emitted from the second slit aperture are incident on the first dichroic mirror and are transmitted by the first dichroic mirror; The control module is electrically connected to the first LED light source unit, the second LED light source unit and the third LED light source unit, and is used to control the power and switching of the first LED light source, the second LED light source and the third LED light source.
2. The multi-wavelength incoherent linear light beam generating device according to claim 1, characterized in that: The first LED light source, the second LED light source and the third LED light source are light sources of different colors.
3. The multi-wavelength incoherent linear light beam generating device according to claim 2, characterized in that: The first LED light source is blue, the second LED light source is green, and the third LED light source is red.
4. The multi-wavelength incoherent linear light beam generating device according to claim 3, characterized in that: When the first LED light source, the second LED light source and the third LED light source are turned on at full power at the same time, a white light beam can be generated; when the first LED light source, the second LED light source and the third LED light source are turned on separately, a monochrome line beam can be generated; when the first LED light source, the second LED light source and the third LED light source are all turned on at a certain proportion of light power, a line beam of a specific color can be generated.
5. The multi-wavelength incoherent linear light beam generating device according to claim 1, characterized in that: The slit widths of the first slit aperture and the second slit aperture can be set to different widths to generate line light beams of different widths.
6. The multi-wavelength incoherent linear light beam generating device according to claim 1, characterized in that: The first condenser, the second condenser and the third condenser are all collimators, and the LED light source is collimated by the corresponding collimator and then irradiated on the corresponding slit diaphragm.
7. The multi-wavelength incoherent linear light beam generating device according to claim 1, characterized in that: The first condenser, the second condenser and the third condenser are all in the form of a combination of a collimator and a cylindrical lens. The LED light source is collimated by the corresponding collimator and focused by the corresponding cylindrical lens before irradiating the corresponding slit aperture.
8. A method for generating a multi-wavelength incoherent linear beam according to claim 1, characterized in that: The steps include: The control module controls the power and switching of the first LED light source, the second LED light source and the third LED light source; The light beam emitted by the first LED light source passes through the first condenser and is irradiated on the first slit diaphragm, and the line light beam emitted from the first slit diaphragm is incident on the first dichroic mirror and reflected by the first dichroic mirror; the light beam emitted by the second LED light source passes through the second condenser and is reflected by the second dichroic mirror and is irradiated on the second slit diaphragm; the light beam emitted by the third LED light source passes through the third condenser and is transmitted by the second dichroic mirror and is irradiated on the second slit diaphragm, and the light beams of the second LED light source and the third LED light source emitted from the second slit diaphragm are incident on the first dichroic mirror and are transmitted by the first dichroic mirror.
9. A line scanning imaging device, characterized in that: The invention comprises the multi-wavelength incoherent linear light beam generating device as described in any one of claims 1 to 7.