Anti-allergic composition, preparation method thereof and application of anti-allergic composition as skin repairing agent

Abstract

The invention relates to the technical field of biological medicine, in particular to an anti-allergic composition, a preparation method thereof and application of the anti-allergic composition as a skin repairing agent, and the anti-allergic composition comprises a Sophora davurica DAVIDII seed extract, an ERIGERON BREVISCAPUS extract and a Dendrobium nobile (DENDROBIUM NOBILE) extract. Meanwhile, the invention further provides the anti-allergic composition, a preparation method of the anti-allergic composition and application of the anti-allergic composition as a skin repairing agent, and the anti-allergic composition is derived from whole plants and can achieve a good synergistic effect in the anti-allergic aspect.

Description

Technical Field

[0001] The present invention relates to the field of biomedicine technology, and in particular to an anti-allergic composition and a preparation method thereof and application thereof as a skin repair agent. Background Art

[0002] Sensitive skin (SS) refers to a highly reactive state of the skin under physiological or pathological conditions. It is a special skin type and is common on the facial skin. Clinically, when stimulated by physical, chemical, mental and other factors, the skin is prone to feelings of burning, stinging, itching and tightness, and may be accompanied by erythema, papules, desquamation, capillary dilation and other conditions. The cause of SS is not yet fully understood. The "Guidelines for Clinical Diagnosis and Treatment of Sensitive Skin in China (2024 Edition)" mentions that frequent changes in cosmetics or the use of multiple brands of cosmetics at the same time, excessive facial cleansing, repeated use of disinfectant products, topical irritant drugs, local long-term topical glucocorticoids, and certain laser treatments can all induce SS.

[0003] At present, there have been a large number of research reports on the use of single plant extracts or a combination of a few plant extracts for anti-allergy. For example, some studies have found that purslane extract has a good soothing effect on skin allergies. The polysaccharides, flavonoids and other ingredients it contains can inhibit the production of inflammatory cytokines and reduce skin inflammatory reactions; other studies have shown that the active ingredients in chamomile extracts, such as apigenin and matricaria, have anti-allergic, anti-inflammatory and antioxidant properties, and can relieve allergic symptoms by regulating the function of immune cells. However, these studies mostly focus on the mechanism of action and efficacy verification of single plant extracts. There are relatively few studies on the combination of multiple plant extracts in specific proportions to form a comprehensive anti-allergic composition. At the same time, new combinations of anti-allergic plant active ingredients are urgently needed to be developed. Summary of the invention

[0004] In order to solve the above technical problems, the purpose of the present invention is to provide an anti-allergic composition and a preparation method thereof and application as a skin repair agent. The anti-allergic composition is derived from whole plants and can play a good synergistic role in anti-allergy.

[0005] In order to achieve the above technical effects, the present invention adopts the following technical solutions: In a first aspect, the present invention provides an antiallergic composition, which comprises the following raw material components: a Sophora DAVIDII seed extract, an Erigeron BREVISCAPUS extract and a Dendrobium nobile extract.

[0006] Preferably, the antiallergic composition comprises the following raw material components in parts by mass: 2 to 7 parts of Sophora DAVIDII seed extract, 10 to 19 parts of Erigeron breviscapus extract and 2 to 7 parts of DENDROBIUM NOBILE extract.

[0007] Further preferably, the antiallergic composition comprises the following raw material components in parts by mass: 3 parts of Sophora DAVIDII seed extract, 14 parts of Erigeron breviscapus extract and 3 parts of DENDROBIUM NOBILE extract.

[0008] In a second aspect, the present invention further provides a method for preparing the aforementioned antiallergic composition, the preparation method comprising at least the following steps: S1: weighing raw material components, wherein the raw material components at least include: Sophora DAVIDII seed extract, Erigeron BREVISCAPUS extract and DENDROBIUM NOBILE extract; S2: Put the raw material components into a mixing container with a stirring device, add a suitable solvent, and fully mix the extracts and the solvent at a temperature below 70°C.

[0009] Preferably, in step S1: The SOPHORA DAVIDII seed extract is prepared by the following method: The dried seeds of the white thorn flower are crushed and placed in an extraction container, and an ethanol solution with a volume fraction of 70% to 80% is added at a solid-liquid ratio of 1:10 to 1:15, and the reflux extraction method is adopted. The extraction is performed 2 to 3 times at 80 to 90°C, and the extraction time for each time is 1.5 to 2.5 hours. The extracts are combined, concentrated under reduced pressure, and dried to obtain the white thorn flower seed extract; The Erigeron breviscapus extract is prepared by the following method: Take the whole dried Erigeron breviscapus herb, cut it into small pieces, put it into an ultrasonic extraction device, add 8 to 10 times the amount of water, set the ultrasonic power to 200 to 300W, the ultrasonic temperature to 40 to 50°C, and the extraction time to 30 to 40 minutes for extraction. After the extraction is completed, filter the extract, and the filtrate is concentrated under reduced pressure and dried to obtain the Erigeron breviscapus extract; The DENDROBIUM NOBILE extract is prepared by the following method: Take dried Dendrobium nobile and grind it, add 5 to 8 times the amount of water, soak it for 1 to 2 hours, and then decoct it for extraction. The decoction temperature is maintained at 95 to 100°C, and the extraction is performed 2 to 3 times, each time for 1 to 1.5 hours; the decoctions are combined and filtered to obtain a filtrate, and the filtrate is concentrated and dried to obtain a Dendrobium nobile extract.

[0010] In a third aspect, the present invention further provides a use of the aforementioned anti-allergic composition as a skin repair agent.

[0011] Preferably, the application is specifically: use of the anti-allergic composition as a skin repairing agent in the preparation of daily chemical products.

[0012] Furthermore, the antiallergic composition is added in an amount of 1 to 10 wt % in the daily chemical product.

[0013] Preferably, the daily chemical product is a facial skin care product.

[0014] In a fourth aspect, based on the aforementioned antiallergic composition, the present invention further provides a facial cream, wherein the facial cream components are as follows, calculated by weight: 3 parts of Sophora DAVIDII seed extract, 14 parts of Erigeron brevicapus extract and 3 parts of Dendrobium nobile extract, 10 parts of sweet almond oil, 5 parts of cetearyl alcohol, 7.5 parts of petrolatum, 450 parts of deionized water, 5 parts of glycerin, 2 parts of polysorbate 80 and 0.5 parts of phenoxyethanol.

[0015] The preparation method of the facial cream is as follows: Accurately weigh the various ingredients in the above formula; heat and stir sweet almond oil, cetearyl alcohol and vaseline to completely melt and mix the oil components evenly, and control the temperature at 70-75°C to obtain the oil phase; then mix deionized water and glycerin evenly, and then heat to 70-75°C, stirring while heating to ensure that glycerin and water are evenly mixed, and set aside as the water phase. Slowly pour the water phase into the oil phase, turn on the stirrer at the same time, stir at medium speed, and stir for about 15-20 minutes to fully emulsify the oil phase and the water phase to form a uniform emulsion. After the temperature drops, add the pre-prepared white thorn flower seed extract, short-stemmed fleabane extract, nobile hairpin extract and phenoxyethanol, continue to stir evenly, and stir for about 5-10 minutes to complete the preparation of the cream.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The present invention provides an antiallergic composition comprising the following active ingredients: White thorn flower seed extract: Modern research shows that white thorn flower seeds contain a variety of alkaloids, flavonoids and other active ingredients. These ingredients have antioxidant and anti-inflammatory effects, can reduce the damage of free radicals to the skin, inhibit the release of inflammatory mediators, thereby relieving skin allergy symptoms and enhancing skin tolerance.

[0017] Erigeron shortstem extract: Erigeron shortstem mainly contains flavonoids, which have strong antioxidant and anti-inflammatory activities. It can remove free radicals in the skin, reduce inflammatory reactions, promote blood circulation in the skin, enhance the skin's self-repair ability, and help improve skin redness, swelling, itching and other problems caused by allergies.

[0018] And Dendrobium nobile extract: Dendrobium nobile is rich in dendrobium polysaccharide, dendrobium alkaloids and other ingredients. Dendrobium polysaccharide has a good moisturizing effect, which can increase the moisture content of the skin and keep the skin moisturized; at the same time, it can also regulate the immune function of the skin, enhance the barrier function of the skin, and reduce the stimulation of external allergens to the skin. Dendrobium alkaloids have certain anti-inflammatory and anti-allergic effects.

[0019] The anti-allergic composition provided by the present invention reasonably compounds the above three components, so that the composition as a whole has the following advantages: First, natural ingredients with high safety: the anti-allergic composition of Whitethorn Seed, Erigeron breviscapus and Dendrobium nobile are all natural plants, and each component has good safety. Their extracts are used in skin care products, which are safer than chemical synthetic ingredients, are less likely to cause skin allergies or other adverse reactions, and are suitable for sensitive skin.

[0020] Second, multi-component synergistic effect: In this anti-allergic composition, the active ingredients of the three extracts work synergistically with each other to play a role in multiple aspects such as anti-oxidation, anti-inflammatory, moisturizing, and strengthening the skin barrier, which can comprehensively improve the skin's allergic conditions and improve the health of the skin.

[0021] Third, it has diverse functions: the anti-allergic composition not only has anti-allergic effects, but also can achieve multiple functions such as moisturizing, anti-oxidation, and repair. While improving skin allergy problems, it can also improve the overall quality of the skin, making the skin smoother, more delicate, and elastic.

[0022] The anti-allergic composition shows good anti-allergic effect in laboratory and human body tests, and there is an obvious synergistic effect between the components. DETAILED DESCRIPTION

[0023] The following embodiments are only used to more clearly illustrate the technical solutions of the present invention and are therefore only used as examples and are not intended to limit the scope of protection of the present invention.

[0024] Example 1 This embodiment provides an anti-allergic composition, which includes the following raw material components: Sophora davidii seed extract, Erigeron breviscapus extract and Dendrobium nobile extract. The preparation method of the anti-allergic composition is as follows: S1: Prepare and weigh the following raw material components respectively by mass: 3 parts of Sophora DAVIDII seed extract, 14 parts of Erigeron BREVISCAPUS extract and 3 parts of DENDROBIUM NOBILE extract, wherein: The Sophora Davidii seed extract was prepared as follows: The dried white thorn flower seeds are crushed and placed in an extraction container, and a 75% ethanol solution is added at a solid-liquid ratio of 1:10 to 1:15, and a reflux extraction method is used to extract 2 to 3 times at 90°C, each extraction time is 2 hours, and the extracts are combined, concentrated under reduced pressure, and dried to obtain a white thorn flower seed extract; The Erigeron breviscapus extract is prepared by the following method: Take the whole dried Erigeron short-stemmed herb, cut it into small pieces and put it into the ultrasonic extraction equipment, add 10 times the amount of water, set the ultrasonic power to 250 W, the ultrasonic temperature to 50 ℃, and the extraction time to 40 minutes for extraction. After the extraction is completed, filter the extract, and the filtrate is concentrated under reduced pressure and dried to obtain the Erigeron short-stemmed extract; The DENDROBIUM NOBILE extract is prepared by the following method: The dried Dendrobium nobile was crushed, 7 times the amount of water was added, and the mixture was soaked for 2 hours, followed by decoction extraction. The decoction temperature was maintained at 95 to 100° C., and the extraction was performed twice, each time for 1.5 hours. The decoctions were combined and filtered to obtain a filtrate, and the filtrate was concentrated and dried to obtain a Dendrobium nobile extract.

[0025] S2: Put the raw material components in S1 into a mixing container with a stirring device, use water as a solvent, and fully mix the extracts with water at below 70° C. to prepare the antiallergic composition I.

[0026] Example 2 This embodiment provides an anti-allergic composition, and the preparation method of the anti-allergic composition is as follows: S1: Prepare and weigh the following raw material components according to the mass proportions respectively according to Example 1: 2 parts of Sophora scabra seed extract, 16 parts of Erigeron breviscapus extract and 2 parts of Dendrobium nobile extract; S2: Put the raw material components in S1 into a mixing container with a stirring device, use water as a solvent, and fully mix the extracts with water at below 70° C. to prepare the antiallergic composition II.

[0027] Example 3

[0028] This embodiment provides an anti-allergic composition, and the preparation method of the anti-allergic composition is as follows: S1: Prepare and weigh the following raw material components according to the mass proportions respectively with reference to Example 1: 5 parts of Sophora scabra seed extract, 10 parts of Erigeron breviscapus extract and 5 parts of Dendrobium nobile extract; S2: Put the raw material components in S1 into a mixing container with a stirring device, use water as a solvent, and fully mix the extracts with water at below 70° C. to prepare the anti-allergic composition III.

[0029] Example 4 This embodiment provides an anti-allergic composition, and the preparation method of the anti-allergic composition is as follows: S1: Prepare and weigh the following raw material components according to the mass fractions: 6 parts of Sophora avidii seed extract and 14 parts of Erigeron breviscapus extract, respectively, with reference to Example 1; S2: Put the raw material components in S1 into a mixing container with a stirring device, use water as a solvent, and fully mix the extracts with water at below 70° C. to prepare the antiallergic composition IV.

[0030] Example 5 This embodiment provides an anti-allergic composition, and the preparation method of the anti-allergic composition is as follows: S1: Prepare and weigh the following raw material components according to the mass ratio according to Example 1: 6 parts of DENDROBIUM NOBILE extract and 14 parts of ERIGERON BREVISCAPUS extract; S2: Put the raw material components in S1 into a mixing container with a stirring device, use water as a solvent, and fully mix the extracts with water at a temperature below 70° C. to prepare the antiallergic composition V.

[0031] Test Example This test example tests the efficacy of the antiallergic compositions provided in Examples 1 to 5, specifically including: 1 Observation of the effects of each composition on atopic dermatitis (AD) in mice 1.1 Experimental materials and groups Experimental animals: Healthy female BALB / c mice, 6-8 weeks old, weighing 18-22 g, were kept in a constant temperature and humidity environment with free access to food and water. The mice were randomly divided into 7 groups, 5 mice in each group, including a normal control group, Example 1 to Example 5 groups, and a model control group. The experimental operation was as follows: 1.2 Modeling and drug administration The model was established by using 2,4-dinitrochlorobenzene (DNCB) solution as a sensitizer (dissolved in a mixed solution of acetone and olive oil (acetone: olive oil = 4:1, v / v)). The specific method is as follows: One day before the start of the experiment, a special animal depilatory cream was used to remove hair on both sides of the mouse spine on the back, and the depilatory area was about 4cm×4cm. From the 1st to the 7th day after depilation, 50μL of 1% DNCB solution was evenly applied to the depilated area on the back of the mouse every day to allow the drug to fully contact the skin. Starting from the second week, 100 μL of 0.2% DNCB solution was applied to the depilated area twice a week for 3 weeks to maintain the dermatitis symptoms. The model was judged to be successful when erythema, papules, edema, and scales appeared on the mouse skin, accompanied by itching.

[0032] Drug administration: The mice in Example 1 group to Example 5 group were administered with anti-allergic composition I to anti-allergic composition V, respectively (anti-allergic composition I to anti-allergic composition V were prepared at 4 wt%), the mice in the normal control group were only smeared with solvent during the entire experiment, and the model control group was not given any therapeutic drugs during the entire modeling process.

[0033] 1.3 Experimental Results and Analysis The dosage of each group was controlled to be consistent, with the dosage frequency being once a day starting from the fourth week of modeling for 7 consecutive days. The single dosage was 50uL, which was evenly applied to the dermatitis area. The efficacy was evaluated after the experiment.

[0034] (01) Observation of skin symptoms The allergic symptoms of the mouse skin were observed and recorded on the 0th, 3rd and 7th day after administration, and scored according to the following scoring criteria: 0 points, no significant change; 1 point, slight redness and swelling; 2 points, moderate redness and swelling, accompanied by mild itching; 3 points, severe redness and swelling, with obvious exudation and scratching marks; After the therapeutic administration, the average symptom score of each group of mice was calculated. The experimental results are shown in Table 1: Table 1 Symptom score table of each group of mice after the end of therapeutic administration

[0035] (02) Scratching score The continuous scratching of the lesion area by the mouse after lifting its paw was counted as 1 time, the scratching again after the mouse put down its paw or paused or licked was counted as the second time, and continuous scratching was counted as 1 time. The statistical time was 10 min. During the observation process, the mice were placed in a transparent polypropylene box with holes, and the environment was kept quiet and unmanned. The camera recorded and counted the scratching times of the mice in each group. The experimental results are shown in Table 2 (0th day of administration, 3rd day of administration, and 7th day of administration): Table 2 Effects of therapeutic drug administration on the scratching scores of mice in each group (times)

[0036] The above experimental results show that the effects of different anti-allergic compositions on the AD response of mice vary greatly, especially the anti-allergic composition I, whose anti-AD effect is significantly different from that of other groups.

[0037] Observe the effects of each composition on 2,4-dinitrochlorobenzene (DNCB)-induced ear swelling in mice 2.1 Experimental Materials and Grouping Referring to Example 1, the number of mice in each group was 7; 2.2 Modeling and drug administration Use a pipette to apply 20 μL of xylene evenly on both sides of the right ear of the mouse, and the left ear is used as a normal control to induce acute ear swelling.

[0038] After modeling, each group of mice was administered with a volume of 10 uL of drugs, the drug concentration was referred to Example 1, and the drug was administered once a day for 3 consecutive days, specifically: Normal control group: no treatment.

[0039] Model control group: xylene was applied only on the right ear to induce inflammation, and the left ear served as a normal control.

[0040] The other five groups were treated with anti-allergic combination I to anti-allergic combination V respectively.

[0041] 2.3 Experimental Results and Analysis (01) Determination of ear swelling On the 0th day (after modeling), 1st day, and 3rd day of administration, the thickness of the same part of the right ear of the mouse was measured with a vernier caliper, and the ear swelling was calculated as follows: Ear swelling (%) = [(right ear thickness - left ear thickness) / left ear thickness] × 100%; The experimental results are shown in Table 3: Table 3 Effects of therapeutic administration on ear swelling of mice in each group (%) Group Day 0 (after modeling) Day 1 Day 3 Normal control group 0.0 ± 0.0 0.0 ± 0.0 0.0 ± 0.0 Model control group 100.0 ± 5.2 105.3 ± 6.1 112.5 ± 7.8 Anti-allergic combination group I 100.3 ± 5.3 44.7 ± 3.5*# 10.4 ± 1.9*# Anti-allergy combination II 99.8 ± 4.9 65.9 ± 3.9* 32.1 ± 2.7* Anti-allergic combination group III 100.1 ± 5.0 72.3 ± 4.2* 43.5 ± 3.5* Anti-allergy combination IV group 99.2 ± 5.1 78.6 ± 4.7* 55.7 ± 3.8* Anti-allergy combination V group 98.5 ± 4.8 88.4 ± 5.3* 75.2 ± 4.9* Note: * indicates P < 0.05 compared with the model control group, # indicates P < 0.05 compared with the treatment group II; (02) Determination of inflammatory factor content in skin tissue After administration for 3 days, the skin tissue of mouse ears was taken, homogenized and centrifuged to obtain the supernatant. The ELISA method was used to detect the content of interleukin-4 (IL-4), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the skin tissue. The specific operation was carried out according to the instructions of the kit. The data were expressed as mean ± standard deviation, and the inflammatory factor level of the model control group was taken as 100%. The experimental results are shown in Table 4: Table 4 Effects of therapeutic administration on the levels of inflammatory factors in the skin tissue of mice in each group (%) Group IL-4 (%) IL-6 (%) TNF-α(%) Normal control group 10.2 ± 1.1 8.5 ± 0.9 9.8 ± 1.0 Model control group 100.0 ± 5.8 100.0 ± 6.2 100.0 ± 7.1 Anti-allergic combination group I 23.6 ± 1.8 20.1 ± 1.5*# 22.4 ± 1.7*# Anti-allergy combination II 37.5 ± 2.5 34.8 ± 2.3* 35.9 ± 2.4* Anti-allergic combination group III 54.7 ± 3.2 49.5 ± 3.0* 51.1 ± 3.1* Anti-allergy combination IV group 68.9 ± 3.9 63.2 ± 3.7* 65.3 ± 3.8* Anti-allergy combination V group 82.4 ± 4.3 75.6 ± 4.1* 78.9 ± 4.5* Note: * indicates P < 0.05 compared with the model control group, # indicates P < 0.05 compared with the treatment group II; The above experimental results show that: In the ear swelling experiment, the ear swelling of the model control group gradually increased over time, and its inflammation was not suppressed), while the swelling of the treatment group decreased significantly as the administration time prolonged, and the anti-allergic composition I had the best effect; In the experiment to observe the effect of therapeutic administration on the content of inflammatory factors in the skin tissue of each group of mice, the content of inflammatory factors (IL-4, IL-6, TNF-α) in the treatment group was significantly reduced, and the anti-allergic combination I had the strongest inhibitory effect, which was significantly different compared with other groups.

[0042] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all known technologies.

Claims

1. An antiallergic composition, characterized in that: The invention comprises the following raw material components: Sophora DAVIDII seed extract, Erigeron BREVISCAPUS extract and Dendrobium NOBILE extract.

2. An antiallergic composition according to claim 1, characterized in that: The invention comprises the following raw material components in parts by weight: 2 to 7 parts of Sophora davidii seed extract, 10 to 19 parts of Erigeron breviscapus extract and 2 to 7 parts of Dendrobium nobile extract.

3. An antiallergic composition according to claim 1, characterized in that: The invention comprises the following raw material components in parts by weight: 3 parts of Sophora DAVIDII seed extract, 14 parts of Erigeron BREVISCAPUS extract and 3 parts of DENDROBIUM NOBILE extract.

4. The method for preparing an antiallergic composition according to any one of claims 1 to 3, characterized in that: At least the following steps are included: S1: weighing raw material components, wherein the raw material components at least include: Sophora DAVIDII seed extract, Erigeron BREVISCAPUS extract and DENDROBIUM NOBILE extract; S2: Put the raw material components into a mixing container with a stirring device, add a suitable solvent, and fully mix the extracts and the solvent at a temperature below 70°C.

5. The method for preparing an antiallergic composition according to claim 4, characterized in that: In step S1: The SOPHORA DAVIDII seed extract is prepared by the following method: The dried white thorn flower seeds are crushed and placed in an extraction container, and an ethanol solution with a volume fraction of 70% to 80% is added at a solid-liquid ratio of 1:10 to 1:15, and a reflux extraction method is adopted to extract 2 to 3 times at 80 to 90° C., and each extraction time is 1.5 to 2.5 hours; the extracts are combined, concentrated under reduced pressure, and dried to obtain a white thorn flower seed extract; The Erigeron breviscapus extract is prepared by the following method: Take the whole dried Erigeron breviscapus herb, cut it into small pieces, put it into an ultrasonic extraction device, add 8 to 10 times the amount of water, set the ultrasonic power to 200 to 300W, the ultrasonic temperature to 40 to 50°C, and the extraction time to 30 to 40 minutes for extraction. After the extraction is completed, filter the extract, and the filtrate is concentrated under reduced pressure and dried to obtain the Erigeron breviscapus extract; The DENDROBIUM NOBILE extract is prepared by the following method: Take dried Dendrobium nobile and grind it, add 5 to 8 times the amount of water, soak it for 1 to 2 hours, and then decoct it for extraction. The decoction temperature is maintained at 95 to 100°C, and the extraction is performed 2 to 3 times, each time for 1 to 1.5 hours; the decoctions are combined and filtered to obtain a filtrate, and the filtrate is concentrated and dried to obtain a Dendrobium nobile extract.

6. Use of the antiallergic composition according to any one of claims 1 to 3 as a skin repairing agent.

7. Use of the antiallergic composition according to any one of claims 1 to 3 as a skin repairing agent in the preparation of daily chemical products.

8. The use according to claim 7, characterized in that: The antiallergic composition is added in an amount of 1 to 10 wt % in the daily chemical product.

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