Nano calcium molybdate as well as preparation method and application thereof

Abstract

The invention discloses nano calcium molybdate as well as a preparation method and application thereof, and the preparation method comprises the step of reacting nano molybdenum trioxide with nano calcium carbonate to obtain the nano calcium molybdate. The nano calcium molybdate can promote the growth of saccharomycetes and has a good promotion effect on the growth of the saccharomycetes.

Description

Technical Field

[0001] The invention belongs to the technical field of nano materials, and more specifically, relates to nano calcium molybdate and a preparation method and application thereof. Background Art

[0002] Yeast (saccharomycetes) is a microorganism widely used in food, beverage, pharmaceutical and bioengineering industries. Due to its rapid reproduction ability, strong metabolic capacity and good industrial production characteristics, it has become a key organism in important fermentation production processes. The reproduction rate and growth state of yeast directly affect the quality and yield of fermentation products. Therefore, how to effectively promote the growth and reproduction of yeast is an important issue in the yeast fermentation industry.

[0003] At present, common methods for promoting yeast reproduction include optimizing the composition of the culture medium, adjusting the temperature and pH value, supplementing nitrogen and carbon sources, etc. For example, growth promoters are added to the culture medium. Metal ions play an important role in many physiological processes in organisms, especially in cell metabolism, enzyme activity, and redox reactions. Certain metal ions, such as zinc, copper, and iron, have been shown to have a positive effect on the growth and reproduction of yeast. However, research in the prior art on how to use metal salts to regulate the reproduction mechanism and growth rate of yeast is still relatively scarce, and there is a lack of effective metal salt additives to improve the production efficiency and economy of yeast. Summary of the invention

[0004] The purpose of the present invention is to provide a nano calcium molybdate and a preparation method and application thereof. The nano calcium molybdate of the present invention can promote the growth of yeast and has a good promoting effect on the growth of yeast.

[0005] In order to achieve the above object, the first aspect of the present invention provides a method for preparing nano-calcium molybdate, which comprises: reacting nano-molybdenum trioxide and nano-calcium carbonate to obtain the nano-calcium molybdate.

[0006] In the present invention, the reaction is preferably a dry reaction.

[0007] According to the present invention, preferably, the molar ratio of the nano-molybdenum trioxide to the nano-calcium carbonate is 1:(1.02-2).

[0008] According to the present invention, preferably, the particle size of the nano molybdenum trioxide is 90-110 nm;

[0009] The particle size of the nano calcium carbonate is 90-110 nm.

[0010] According to the present invention, preferably, the reaction temperature is 500-600° C. and the reaction time is 4-8 h.

[0011] In the present invention, as a preferred embodiment, the preparation method of the present invention comprises: mixing nano calcium molybdate and nano calcium carbonate in a molar ratio of 1:1.02, and dry reacting at a temperature of 560 degrees for 6 hours.

[0012] The second aspect of the present invention provides nano calcium molybdate prepared by the above preparation method.

[0013] According to the present invention, preferably, the nano calcium molybdate is elliptical.

[0014] According to the present invention, preferably, the particle size of the nano calcium molybdate is 50-80 nm.

[0015] The third aspect of the present invention provides the use of the above-mentioned nano calcium molybdate as a yeast growth promoter.

[0016] According to the present invention, preferably, the nano-calcium molybdate is added to the culture medium in the form of a nano-calcium molybdate aqueous solution, the volume ratio of the nano-calcium molybdate aqueous solution to the yeast culture medium is 50:(5-7), the concentration of the nano-calcium molybdate aqueous solution is 50-200ppm, and the OD value of the yeast culture medium is 0.9-1.2.

[0017] The technical solution of the present invention has the following beneficial effects: the nano calcium molybdate of the present invention can promote the growth of yeast and has a good promoting effect on the growth of yeast.

[0018] Other features and advantages of the present invention will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The above and other objects, features and advantages of the present invention will become more apparent through a more detailed description of exemplary embodiments of the present invention in conjunction with the accompanying drawings, wherein like reference numerals generally represent like components throughout the exemplary embodiments of the present invention.

[0020] Figure 1 The XRD diagram of nano-CaMoO4 according to a test example of the present invention is shown; wherein, Indensity represents intensity, and degree represents angle.

[0021] Figure 2 A SEM scan of nano-CaMoO4 according to a test example of the present invention is shown.

[0022] Figure 3A laboratory test of nano-CaMoO4 promoting yeast growth according to a test example of the present invention is shown. Among them, (a)(b), (c)(d), (e)(f), (g)(h), (i)(j), (k)(l), (m)(n) are a group of experiments respectively. And the last figure of each group of experiments is the result after the culture dish of the previous figure is left to stand for 24 hours. Two groups of experiments are set for each concentration. Among them, ck represents the control group. DETAILED DESCRIPTION

[0023] The preferred embodiments of the present invention will be described in more detail below. Although the preferred embodiments of the present invention are described below, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to make the present invention more thorough and complete, and to fully convey the scope of the present invention to those skilled in the art.

[0024] The present invention is further described below by examples:

[0025] The particle size of the nano-molybdenum trioxide used in the following examples is 100 nm, and the particle size of the nano-calcium carbonate is 100 nm.

[0026] Example

[0027] After mixing nano molybdenum trioxide and nano calcium carbonate in a molar ratio of 1:1.02, place them in a material boat, send the material boat into a preheated tubular furnace, and dry react at 560°C for 6 hours. After cooling, take out the material boat to obtain pure nano calcium molybdate.

[0028] Test Example 1

[0029] The nano calcium molybdate prepared in the embodiment was subjected to XRD analysis, and the test results are as follows: Figure 1 shown by Figure 1 It can be seen that the synthetic product of the embodiment is calcium molybdate.

[0030] Test Example 2

[0031] The nano calcium molybdate prepared in the example was scanned by electron microscope, and the test results are as follows: Figure 2 As shown. Figure 2 It can be seen that nano calcium molybdate is oval in shape and has a particle size of 50-80nm.

[0032] Test Example 3

[0033] Preparation of 50ppm, 100ppm and 200ppm concentrations of calcium molybdate aqueous solution: 10ml of ultrapure water and a measured amount of nano-CaMoO4 prepared in the above embodiment are mixed, and the solution is shaken for 10min to mix well to obtain a calcium molybdate aqueous solution of the target concentration.

[0034] The promoting effect of nano-CaMoO4 on yeast was studied, and its promoting effect was tested in the laboratory through Y187 yeast competent state. Y187 yeast competent state was purchased from Weidi Biology, and YPDA medium (mainly used for the cultivation of yeast in molecular biology experiments) was selected. After high-temperature sterilization of YPDA medium, calcium molybdate aqueous solutions with concentrations of 50ppm, 100ppm and 200ppm were added to the medium, and the amount of calcium molybdate aqueous solution added was 50μL. After it was naturally dried, an equal amount of yeast liquid with the same OD value was absorbed, and the yeast was inoculated by the streaking method (the OD value of the yeast liquid was 1.0, and the coating amount on the culture medium was 6μL). It was cultured in a sterile incubator under a dark environment at 28°C for 24 hours; among them, two experimental groups were set up for each concentration of calcium molybdate aqueous solution. Figure 3 As shown, it was found that the calcium molybdate aqueous solution had the best promoting effect on the growth of yeast at a concentration of 100 ppm, and had a certain promoting effect on the growth of yeast at 50 ppm and 200 ppm, but it was not very obvious.

[0035] The embodiments of the present invention have been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments.

Claims

1. A method for preparing nano calcium molybdate, characterized in that: The preparation method comprises: reacting nano molybdenum trioxide and nano calcium carbonate to obtain the nano calcium molybdate.

2. The preparation method according to claim 1, wherein The molar ratio of the nano molybdenum trioxide to the nano calcium carbonate is 1:(1.02-2).

3. The preparation method according to claim 1, wherein The particle size of the nano molybdenum trioxide is 90-110nm; The particle size of the nano calcium carbonate is 90-110 nm.

4. The preparation method according to claim 1, wherein The reaction temperature is 500-600°C and the reaction time is 4-8h.

5. Nano calcium molybdate prepared by the preparation method described in any one of claims 1 to 4.

6. The nano calcium molybdate according to claim 5, wherein: The nano calcium molybdate is oval.

7. The nano calcium molybdate according to claim 5, wherein: The particle size of the nano calcium molybdate is 50-80nm.

8. Use of the nano calcium molybdate described in any one of claims 5 to 7 as a yeast growth promoter.

9. The use according to claim 8, wherein: The nano calcium molybdate is added to the culture medium in the form of a nano calcium molybdate aqueous solution, the volume ratio of the nano calcium molybdate aqueous solution to the yeast culture liquid is 50:(5-7), the concentration of the nano calcium molybdate aqueous solution is 50-200 ppm, and the OD value of the yeast culture liquid is 0.9-1.2.

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