Microorganism partitioned culture method, equipment and storage medium

Abstract

The invention provides a microbial partitioned culture method and equipment and a storage medium, and relates to the technical field of microbial culture. The microbial partitioned culture method comprises the following steps: S1, dividing an incubator into a plurality of partitions; s2, an independent environmental parameter control system is arranged in each partition; s3, configuring a corresponding environmental parameter standard interval for each partition before culturing the microorganisms; and S4, in the microbial culture process, periodically detecting the environmental parameters of each subarea, and respectively controlling the environmental parameters of each subarea within the corresponding environmental parameter standard interval through an environmental parameter control system. According to the method, the environmental parameters of each subarea are periodically controlled to be within the set standard interval, so that cross infection caused by different microorganisms in the culture process is avoided, and meanwhile, adaptive environmental parameters can be configured for different microorganisms.

Description

Technical Field

[0001] The present application relates to the technical field of microbial cultivation, and in particular to a microbial zoning cultivation method, equipment and storage medium. Background Art

[0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms. Microorganisms include bacteria, viruses, fungi, etc. They are widely distributed in nature, with large numbers and many types. They can benefit humans or cause diseases. Most microorganisms can be cultured artificially, that is, they are inoculated on culture media to grow and reproduce. The cultured microorganisms are used for research, identification and application. Existing microbial culture sometimes culture different microorganisms in the same incubator, which is prone to cross-infection.

[0003] In addition, when cultivating different microorganisms in the same incubator, the required environmental parameters such as temperature, humidity, carbon dioxide concentration, etc. are different. How to control the cultivation environment parameters of these different microorganisms is one of the problems that need to be solved urgently. Summary of the invention

[0004] The present application provides a microbial zoning culture method, equipment and storage medium to at least solve the above technical problems existing in the prior art.

[0005] According to the first aspect of the present application, a method for culturing microorganisms by partitioning is provided, comprising the following steps: S1, divide the incubator into several partitions; S2, set up an independent environmental parameter control system in each partition; S3, before culturing microorganisms, configure the corresponding standard range of environmental parameters for each partition; S4, during the microbial cultivation process, the environmental parameters of each partition are periodically detected, and the environmental parameters of each partition are controlled within the corresponding environmental parameter standard range through the environmental parameter control system.

[0006] In certain embodiments of the first aspect of the present application, the environmental parameter includes at least one of temperature, humidity, and carbon dioxide concentration.

[0007] In certain embodiments of the first aspect of the present application, the standard interval of the environmental parameter includes a lower threshold value TH1 and an upper threshold value TH2.

[0008] In certain embodiments of the first aspect of the present application, a method for controlling the environmental parameters of each partition within the corresponding environmental parameter standard range by using the environmental parameter control system is as follows: Compare the detected environmental parameters with the corresponding lower threshold TH1 and upper threshold TH2; If the detected environmental parameter is less than the lower threshold TH1 or greater than the upper threshold TH2, the environmental parameter control system changes the power to make the environmental parameter move closer to the environmental parameter standard range; If the detected environmental parameter is between the lower threshold value TH1 and the upper threshold value TH2, the environmental parameter control system keeps the current power unchanged.

[0009] In certain embodiments of the first aspect of the present application, the method in which the environmental parameter control system changes the power so that the environmental parameter moves closer to the environmental parameter standard range is as follows: Pre-test the proportional relationship k1 between the unit power change ΔP of the environmental parameter control system and the unit change ΔTH of the environmental parameter, that is, k1=ΔP / ΔTH; Calculate the difference DTH between the currently detected environmental parameter TH0 and the parameter TH3 within the standard range of environmental parameters, that is, DTH=TH0-TH3; The power change P of the environmental parameter control system is calculated by multiplying the proportional relationship k1 by the difference DTH, that is, P=k1×DTH; According to the calculated power change P, the power of the environmental parameter control system is adjusted.

[0010] In certain embodiments of the first aspect of the present application, the parameter TH3 is equal to the middle value of the lower threshold TH1 and the upper threshold TH2, that is, TH3=(TH1+TH2) / 2.

[0011] In certain embodiments of the first aspect of the present application, if the detected environmental parameter is less than the lower threshold value TH1 or greater than the upper threshold value TH2, the interval of periodic detection is shortened, and after the detected environmental parameter is between the lower threshold value TH1 and the upper threshold value TH2, the interval of periodic detection is restored.

[0012] According to a second aspect of the present application, an electronic device is provided, including: at least one processor; and a memory communicatively connected to the at least one processor; wherein, The memory stores instructions that can be executed by the at least one processor, and the instructions are executed by the at least one processor to enable the at least one processor to perform the method described in the present application.

[0013] According to a third aspect of the present application, a non-transitory computer-readable storage medium storing computer instructions is provided, wherein the computer instructions are used to enable the computer to execute the method described in the present application.

[0014] Compared with the prior art, this application has the following beneficial effects: 1. The present application divides the incubator into several partitions and sets an independent environmental parameter control system for each partition, periodically controlling the environmental parameters of each partition within the set standard range, thereby avoiding cross-infection of different microorganisms during the culture process, and at the same time being able to configure appropriate environmental parameters for different microorganisms.

[0015] 2. This application periodically detects the environmental parameters of the partitions and compares them with the set environmental parameter standard range, so as to accurately control the environmental parameters of each partition within the corresponding environmental parameter standard range, thereby realizing automatic control of the environmental parameters of each partition.

[0016] It should be understood that the content described in this section is not intended to identify the key or important features of the embodiments of the present application, nor is it intended to limit the scope of the present application. Other features of the present application will become easily understood through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] By reading the detailed description below with reference to the accompanying drawings, the above and other purposes, features and advantages of the exemplary embodiments of the present application will become readily understood. In the accompanying drawings, several embodiments of the present application are shown in an exemplary and non-limiting manner, wherein: In the drawings, the same or corresponding reference numerals represent the same or corresponding parts.

[0018] Figure 1 A schematic diagram of the implementation process of the microbial zoning culture method according to an embodiment of the present application is shown.

[0019] Figure 2 A schematic diagram of the structure of an electronic device according to an embodiment of the present application is shown. DETAILED DESCRIPTION

[0020] In order to make the purpose, features, and advantages of the present application more obvious and easy to understand, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present application.

[0021] Embodiment 1: This embodiment provides a method for culturing microorganisms in different zones, comprising the following steps: S1, divide the incubator into several independent partitions, and physically isolate different partitions to prevent cross-infection of microorganisms in each partition.

[0022] S2, an independent environmental parameter control system is set in each partition, so as to realize the corresponding environmental parameters for the cultivation of microorganisms in each partition, wherein the environmental parameters include at least one of temperature, humidity, and carbon dioxide concentration. Taking temperature as an example, the corresponding environmental parameter control system is a refrigerator, such as a small air-conditioning compressor.

[0023] S3, before culturing microorganisms, a corresponding standard range of environmental parameters is configured for each partition, specifically, the configuration is performed according to the type and characteristics of the microorganisms cultured in the partition; the standard range of environmental parameters includes a lower threshold TH1 and an upper threshold TH2.

[0024] Taking temperature as an example, the standard temperature range is [TH1, TH2], where TH1 is the lower temperature threshold and TH2 is the upper temperature threshold.

[0025] S4, during the microbial cultivation process, the environmental parameters of each partition are periodically detected, and the environmental parameters of each partition are controlled within the corresponding environmental parameter standard range through the environmental parameter control system.

[0026] Specifically, the default time interval T0 of periodic detection can be 5 minutes or 10 minutes. The shorter the time interval, the more intensive the environmental parameter detection in the partition. Combined with the regulation of the environmental parameter control system, the cultivation environment of the microorganisms in the partition can be more accurately controlled.

[0027] The method of controlling the environmental parameters of each partition within the corresponding environmental parameter standard range through the environmental parameter control system is as follows: Compare the detected environmental parameter S with the corresponding lower threshold TH1 and upper threshold TH2; If the detected environmental parameter S is less than the lower threshold TH1 or greater than the upper threshold TH2, it means that the environmental parameters in the partition deviate from the normal culture conditions of the microorganisms. Therefore, the environment needs to be regulated, and the environmental parameter control system changes the power to make the environmental parameters move closer to the standard range of environmental parameters.

[0028] The specific method is as follows: Pre-test the proportional relationship k1 between the unit power change ΔP of the environmental parameter control system and the unit change ΔTH of the environmental parameter, that is, k1=ΔP / ΔTH; Calculate the difference DTH between the currently detected environmental parameter TH0 and the parameter TH3 within the standard range of environmental parameters, that is, DTH=TH0-TH3; Preferably, the parameter TH3 is equal to the middle value of the lower threshold TH1 and the upper threshold TH2, that is, TH3=(TH1+TH2) / 2.

[0029] The power change P of the environmental parameter control system is calculated by multiplying the proportional relationship k1 by the difference DTH, that is, P=k1×DTH; According to the calculated power change P, the power of the environmental parameter control system is adjusted.

[0030] Let’s take temperature as an example. Pre-test the proportional relationship k1 between the unit power change ΔP and the unit temperature change ΔTH of the refrigerator, that is, k1=ΔP / ΔTH; Calculate the difference DTH between the currently detected temperature TH0 and the parameter TH3 in the temperature standard interval [TH1, TH2], that is, DTH=TH0-TH3; The power change P of the refrigerator is calculated by multiplying the proportional relationship k1 by the difference DTH, that is, P=k1×DTH; By adjusting the power of the refrigerator according to the calculated power change P, the temperature in the partition can be controlled to the standard temperature range [TH1, TH2] in a short time.

[0031] In addition, in order to verify the result of the regulation as soon as possible after the regulation, the present embodiment also adjusts the time interval of the periodic detection. The specific method is: if the detected environmental parameter is less than the lower threshold TH1 or greater than the upper threshold TH2, the interval of the periodic detection is shortened to T1, and T1 < T0, and after the detected environmental parameter is between the lower threshold TH1 and the upper threshold TH2, the interval of the periodic detection is restored to reduce the overall power consumption of the detection system.

[0032] If the detected environmental parameters are between the lower threshold TH1 and the upper threshold TH2, it means that the current environmental parameters meet the cultivation of the corresponding microorganisms, and the environmental parameter control system maintains the current power unchanged.

[0033] Through the above method, the zoning cultivation of microorganisms can be achieved to avoid cross infection of different microorganisms during the cultivation process, and at the same time, appropriate environmental parameters can be configured for different microorganisms.

[0034] Embodiment 2: According to an embodiment of the present application, the present application also provides an electronic device and a readable storage medium.

[0035] Figure 2A schematic block diagram of an example electronic device that can be used to implement an embodiment of the present application is shown. The electronic device is intended to represent various forms of digital computers, such as laptop computers, desktop computers, workbenches, personal digital assistants, servers, blade servers, mainframe computers, and other suitable computers. The electronic device can also represent various forms of mobile devices, such as personal digital processing, cellular phones, smart phones, wearable devices, and other similar computing devices. The components shown herein, their connections and relationships, and their functions are merely examples and are not intended to limit the implementation of the present application described and / or required herein.

[0036] like Figure 2 As shown, the device includes a computing unit, which can perform various appropriate actions and processes according to a computer program stored in a read-only memory (ROM) or a computer program loaded from a storage unit into a random access memory (RAM). In the RAM, various programs and data required for the operation of the device can also be stored. The computing unit, ROM, and RAM are connected to each other through a bus. An input / output (I / O) interface is also connected to the bus.

[0037] Multiple components in the device are connected to the I / O interface, including: input units, such as keyboards, mice, etc.; output units, such as various types of displays, speakers, etc.; storage units, such as disks, optical disks, etc.; and communication units, such as network cards, modems, wireless communication transceivers, etc. The communication unit allows the device to exchange information / data with other devices through computer networks such as the Internet and / or various telecommunication networks.

[0038] The computing unit may be a variety of general and / or special processing components with processing and computing capabilities. Some examples of computing units include, but are not limited to, central processing units (CPUs), graphics processing units (GPUs), various dedicated artificial intelligence (AI) computing chips, various computing units running machine learning model algorithms, digital signal processors (DSPs), and any appropriate processors, controllers, microcontrollers, etc. The computing unit performs the various methods and processes described above, such as the microbial partitioning culture method described in Example 1. For example, in some embodiments, the microbial partitioning culture method may be implemented as a computer software program, which is tangibly contained in a machine-readable medium, such as a storage unit. In some embodiments, part or all of the computer program may be loaded and / or installed on the device via a ROM and / or a communication unit. When the computer program is loaded into RAM and executed by the computing unit, one or more steps of the microbial partitioning culture method described above may be performed. Alternatively, in other embodiments, the computing unit may be configured to perform the microbial partitioning culture method by any other appropriate means (e.g., by means of firmware).

[0039] Various implementations of the systems and techniques described above herein can be implemented in digital electronic circuit systems, integrated circuit systems, field programmable gate arrays (FPGAs), application specific integrated circuits (ASICs), application specific standard products (ASSPs), systems on chips (SOCs), load programmable logic devices (CPLDs), computer hardware, firmware, software, and / or combinations thereof. These various implementations can include: being implemented in one or more computer programs that can be executed and / or interpreted on a programmable system including at least one programmable processor, which can be a special purpose or general purpose programmable processor that can receive data and instructions from a storage system, at least one input device, and at least one output device, and transmit data and instructions to the storage system, the at least one input device, and the at least one output device.

[0040] The program code for implementing the method of the present application can be written in any combination of one or more programming languages. These program codes can be provided to a processor or controller of a general-purpose computer, a special-purpose computer, or other programmable data processing device, so that the program code, when executed by the processor or controller, implements the functions / operations specified in the flow chart and / or block diagram. The program code can be executed entirely on the machine, partially on the machine, partially on the machine and partially on a remote machine as a stand-alone software package, or entirely on a remote machine or server.

[0041] In the context of the present application, a machine-readable medium may be a tangible medium that may contain or store a program for use by or in conjunction with an instruction execution system, device, or equipment. A machine-readable medium may be a machine-readable signal medium or a machine-readable storage medium. A machine-readable medium may include, but is not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, device, or device, or any suitable combination of the foregoing. A more specific example of a machine-readable storage medium may include an electrical connection based on one or more lines, a portable computer disk, a hard disk, a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disk read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the foregoing.

[0042] To provide interaction with a user, the systems and techniques described herein can be implemented on a computer having: a display device (e.g., a CRT (cathode ray tube) or LCD (liquid crystal display) monitor) for displaying information to the user; and a keyboard and pointing device (e.g., a mouse or trackball) through which the user can provide input to the computer. Other types of devices can also be used to provide interaction with the user; for example, the feedback provided to the user can be any form of sensory feedback (e.g., visual feedback, auditory feedback, or tactile feedback); and input from the user can be received in any form (including acoustic input, voice input, or tactile input).

[0043] The systems and techniques described herein may be implemented in a computing system that includes back-end components (e.g., as a data server), or a computing system that includes middleware components (e.g., an application server), or a computing system that includes front-end components (e.g., a user computer with a graphical user interface or a web browser through which a user can interact with implementations of the systems and techniques described herein), or a computing system that includes any combination of such back-end components, middleware components, or front-end components. The components of the system may be interconnected by any form or medium of digital data communication (e.g., a communication network). Examples of communication networks include: a local area network (LAN), a wide area network (WAN), and the Internet.

[0044] A computer system may include a client and a server. The client and the server are generally remote from each other and usually interact through a communication network. The relationship of client and server is generated by computer programs running on respective computers and having a client-server relationship with each other. The server may be a cloud server, a server of a distributed system, or a server combined with a blockchain.

[0045] It should be understood that the various forms of processes shown above can be used to reorder, add or delete steps. For example, the steps recorded in this application can be executed in parallel, sequentially or in different orders, as long as the expected results of the technical solution of this application can be achieved, and this document is not limited here.

[0046] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0047] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art who is familiar with the present technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. A method for culturing microorganisms in different zones, characterized in that: The following steps are involved: S1, divide the incubator into several partitions; S2, set up an independent environmental parameter control system in each partition; S3, before culturing microorganisms, configure the corresponding standard range of environmental parameters for each partition; S4, during the microbial cultivation process, the environmental parameters of each partition are periodically detected, and the environmental parameters of each partition are controlled within the corresponding environmental parameter standard range through the environmental parameter control system.

2. The microbial zoning culture method according to claim 1, characterized in that: The environmental parameters include at least one of temperature, humidity, and carbon dioxide concentration.

3. The microbial zoning culture method according to claim 1, characterized in that: The environmental parameter standard interval includes a lower threshold value TH1 and an upper threshold value TH2.

4. The microbial zoning culture method according to claim 3, characterized in that: The method of controlling the environmental parameters of each partition within the corresponding environmental parameter standard range through the environmental parameter control system is as follows: Compare the detected environmental parameters with the corresponding lower threshold TH1 and upper threshold TH2; If the detected environmental parameter is less than the lower threshold TH1 or greater than the upper threshold TH2, the environmental parameter control system changes the power to make the environmental parameter move closer to the environmental parameter standard range; If the detected environmental parameter is between the lower threshold value TH1 and the upper threshold value TH2, the environmental parameter control system keeps the current power unchanged.

5. The microbial zoning culture method according to claim 4, characterized in that: The method for the environmental parameter control system to change the power and make the environmental parameters move closer to the environmental parameter standard range is as follows: Pre-test the proportional relationship k1 between the unit power change ΔP of the environmental parameter control system and the unit change ΔTH of the environmental parameter, that is, k1=ΔP / ΔTH; Calculate the difference DTH between the currently detected environmental parameter TH0 and the parameter TH3 within the standard range of environmental parameters, that is, DTH=TH0-TH3; The power change P of the environmental parameter control system is calculated by multiplying the proportional relationship k1 by the difference DTH, that is, P=k1×DTH; According to the calculated power change P, the power of the environmental parameter control system is adjusted.

6. The microbial zoning culture method according to claim 4, characterized in that: The parameter TH3 is equal to the middle value of the lower threshold TH1 and the upper threshold TH2, that is, TH3=(TH1+TH2) / 2.

7. The microbial zoning culture method according to claim 4, characterized in that: If the detected environmental parameter is less than the lower threshold TH1 or greater than the upper threshold TH2, the periodic detection interval is shortened, and after the detected environmental parameter is between the lower threshold TH1 and the upper threshold TH2, the periodic detection interval is restored.

8. An electronic device, characterized in that: include: at least one processor; as well as a memory communicatively connected to the at least one processor; wherein, The memory stores instructions that can be executed by the at least one processor, and the instructions are executed by the at least one processor to enable the at least one processor to perform the method according to any one of claims 1 to 7.

9. A non-transitory computer-readable storage medium storing computer instructions, characterized in that: The computer instructions are used to cause the computer to execute the method according to any one of claims 1 to 7.

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