Electrolyte ion concentration measurement method and analysis equipment

Abstract

The invention discloses an electrolyte ion concentration measurement method and analysis equipment, and relates to the technical field of electrolyte ion concentration detection. Comprising the following steps: measuring a potential value Eb of a mixed solution of an internal standard solution and a buffer solution, a potential value Els of a mixed solution of a low-value calibration solution and the buffer solution, a potential value Ehs of a mixed solution of a high-value calibration solution and the buffer solution, and a potential value Es of a mixed solution of a sample solution and the buffer solution; according to the potential value Eb of the mixed solution of the internal standard solution and the buffer solution, the potential value Els of the mixed solution of the low-value calibration solution and the buffer solution, the potential value Ehs of the mixed solution of the high-value calibration solution and the buffer solution and the potential value Es of the mixed solution of the sample solution and the buffer solution, the electrolyte concentration Cs of the sample solution is obtained; according to the electrolyte ion concentration measurement method and analysis equipment, the internal standard solution is introduced, and the sample solution measurement result and the internal standard solution measurement result are compared and calculated, so that the influence of temperature fluctuation on the final result is eliminated.

Description

Technical Field

[0001] The present invention relates to the technical field of electrolyte ion concentration detection, and more specifically, to a method for measuring electrolyte ion concentration. In addition, the present invention also relates to an electrolyte analysis device for implementing the above electrolyte ion concentration measurement method. Background Art

[0002] The electrolyte concentration measurement in high-speed biochemical analyzers generally uses the indirect ion method to dilute the sample before measurement. Ion-selective electrodes have high sensitivity, fast measurement speed, and require less sample volume.

[0003] However, the measurement results need to be calculated based on the Nernst equation, which is easily affected by the temperature fluctuation of the solution, which may cause inaccurate test results.

[0004] Therefore, the prior art adopts a method of setting a constant temperature device in the liquid filling pipeline to keep the solution at a constant temperature, and adding a temperature measuring device to the electrode assembly to collect the temperature of the solution when passing through the electrode assembly, and then performing temperature compensation. This makes the overall structure and calculation method complicated, which is not conducive to rapid implementation and has limited improvement on the accuracy of the final calculation result.

[0005] In summary, how to solve the problem that the electrolyte ion concentration measurement is affected by temperature fluctuations, resulting in a decrease in the accuracy of the final result, is an urgent problem to be solved by technical personnel in this field. Summary of the invention

[0006] In view of this, the purpose of the present invention is to provide a method for measuring electrolyte ion concentration, by introducing an internal standard solution, comparing and calculating the measurement results of the sample solution with the measurement results of the internal standard solution, thereby eliminating the influence of temperature fluctuations on the final result.

[0007] Another object of the present invention is to provide an electrolyte analysis device for implementing the above-mentioned electrolyte ion concentration measurement method, eliminating the constant temperature module, simplifying the system, and adding a degassing module, further reducing the impact of the gas content in the solution on the effectiveness of the final result.

[0008] In order to achieve the above object, the present invention provides the following technical solutions:

[0009] A method for measuring electrolyte ion concentration, comprising:

[0010] Measure the potential value Eb of the mixture of internal standard solution and buffer solution,

[0011] The potential value Els of the mixture of low-value calibration solution and buffer solution,

[0012] The potential value Ehs of the mixture of high value calibration solution and buffer solution,

[0013] The potential value Es of the mixture of sample solution and buffer solution;

[0014] The electrolyte concentration Cs of the sample solution is obtained according to the nominal concentration Ch of the high-value calibration solution, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the internal standard solution and buffer solution, the potential value Els of the low-value calibration solution and buffer solution, the potential value Ehs of the high-value calibration solution and buffer solution, and the potential value Es of the sample solution and buffer solution.

[0015] Preferably, the electrolyte concentration Cs of the sample solution is obtained according to the nominal concentration Ch of the high-value calibration solution, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the internal standard solution and the buffer solution, the potential value Els of the low-value calibration solution and the buffer solution, the potential value Ehs of the high-value calibration solution and the buffer solution, and the potential value Es of the sample solution and the buffer solution, and further comprises:

[0016] According to the potential value Eb of the internal standard solution and the buffer solution mixture, the potential value Els of the low-value calibration solution and the buffer solution mixture, and the potential value Ehs of the high-value calibration solution and the buffer solution mixture, a calibration slope value SLOP is obtained;

[0017] According to the calibration slope value SLOP, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the internal standard solution and the buffer mixture, and the potential value Els of the low-value calibration solution and the buffer mixture, the actual concentration CM1 of the internal standard solution is obtained;

[0018] The electrolyte concentration Cs of the sample solution is obtained according to the potential value Eb of the mixture of the internal standard solution and the buffer solution, the potential value Es of the mixture of the sample solution and the buffer solution, the actual concentration CM1 of the internal standard solution and the calibration slope value SLOP.

[0019] Preferably, the calibration slope value SLOP is obtained according to the potential value Eb of the internal standard solution and the buffer mixture, the potential value Els of the low-value calibration solution and the buffer mixture, and the potential value Ehs of the high-value calibration solution and the buffer mixture;

[0020] According to the calibration slope value SLOP, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the internal standard solution and the buffer mixture, and the potential value Els of the low-value calibration solution and the buffer mixture, the actual concentration CM1 of the internal standard solution is obtained;

[0021] According to the potential value Eb of the mixture of the internal standard solution and the buffer solution, the potential value Es of the mixture of the sample solution and the buffer solution, the actual concentration CM1 of the internal standard solution and the calibration slope value SLOP, the electrolyte concentration Cs of the sample solution is obtained, further comprising:

[0022] According to the formula:

[0023]

[0024] The electrolyte concentration Cs of the sample solution is obtained.

[0025] Preferably, it also includes,

[0026] According to the potential value Eb of the mixture of the internal standard solution and the buffer solution, the potential value Els of the mixture of the low-value calibration solution and the buffer solution, the potential value Ehs of the mixture of the high-value calibration solution and the buffer solution, the nominal concentration Ch of the high-value calibration solution, the nominal concentration Cl of the low-value calibration solution and the nominal concentration CM of the internal standard solution, a solution factor d is obtained;

[0027] The validity of the electrolyte concentration Cs of the sample solution is determined according to the value of the solution factor d.

[0028] Preferably, judging the validity of the electrolyte concentration Cs of the sample solution according to the value of the solution factor d further includes:

[0029] According to the formula:

[0030]

[0031] Obtaining the solution factor d;

[0032] like , then the value of Cs is judged to be valid, otherwise, the value of Cs is judged to be invalid.

[0033] An electrolyte analysis device, used to implement any one of the above-mentioned electrolyte ion concentration measurement methods, comprising:

[0034] A mixing tank, comprising a mixing chamber, a liquid inlet and a liquid discharge port connected to each other, wherein the liquid inlet is provided with at least two groups of liquid injection mechanisms, and the liquid discharge port is provided with at least one group of liquid discharge mechanisms, wherein a pipe for the sample liquid to flow in the liquid discharge mechanism is serially connected with an electrode assembly for measuring the potential value of the sample liquid;

[0035] A stirring mechanism, disposed in the mixing chamber, for stirring the sample liquid in the mixing chamber;

[0036] The degassing module is arranged at the inlet end of the liquid injection mechanism and is used for separating and removing the gas in the liquid in the liquid injection mechanism.

[0037] Preferably, it further comprises a syringe and a stepping motor, wherein the syringe is provided with a plurality of independent piston chambers, and the stepping motor is used to drive the pistons in the piston chambers to move;

[0038] The injection mechanism and the discharge mechanism both include a two-position three-way electromagnetic reversing valve, the piston chamber is connected to the common end interface of the electromagnetic reversing valve in a one-to-one correspondence, the normally closed port of the electromagnetic reversing valve is connected to the mixing tank, and the normally open port of the electromagnetic reversing valve is used to connect the internal standard solution supply device, the buffer solution supply device or the waste liquid storage device.

[0039] Preferably, the degassing module comprises a connected liquid inlet hole, a degassing cavity and a liquid discharge hole, a negative pressure hole is provided on the top of the degassing cavity, a degassing pump is connected in series to the negative pressure hole, and a degassing membrane is provided at one end of the degassing cavity near the liquid inlet hole.

[0040] Preferably, the mixing chamber is a circular cavity with a smooth inner wall;

[0041] The stirring mechanism comprises a stirring head arranged in the mixing chamber; the stirring head is a strip-shaped structure, and the end of the stirring head is provided with a chamfer;

[0042] The stirring mechanism further comprises a stirring motor arranged outside the mixing chamber, and a magnetic torque transmission mechanism is arranged between the output shaft end of the stirring motor and the stirring head.

[0043] Preferably, a rotating shaft is fixedly provided at the rotating axis of the stirring head, and the rotating shaft is rotatably mounted with respect to the axis position of the mixing chamber.

[0044] Preferably, an inverted conical liquid collecting trough is provided at the bottom of the mixing chamber, a downwardly inclined flow channel is provided between the liquid collecting trough and the liquid discharge port, and the electrode assembly is integrated in the flow channel.

[0045] Compared with the prior art, the electrolyte ion concentration measurement method provided by the present invention has at least the following beneficial effects:

[0046] 1. Introduce the internal standard solution, measure the potential value of the mixture of the internal standard solution and the buffer solution, and then measure the potential value of the mixture of the sample solution and the buffer solution. Through the comprehensive calculation of the two sets of potential values, the influence of temperature fluctuation on the final result is eliminated, which effectively improves the validity of the final result.

[0047] 2. At the same time, the actual calculated internal standard concentration value and calibration slope value of the internal standard solution are introduced into the calculation formula of the electrolyte concentration to further reduce the influence of the error between the actual concentration and the nominal concentration of the internal standard solution on the validity of the final result.

[0048] The electrolyte analysis device provided by the present invention is used to implement the above-mentioned electrolyte ion concentration measurement method and has the following beneficial effects:

[0049] By adding a degassing mechanism, the solution entering the mixing tank is degassed, further reducing the impact of the gas content in the solution on the effectiveness of the final result;

[0050] At the same time, the degassing mechanism is arranged at the inlet end of the liquid injection mechanism, which can shorten the solution transmission path between the degassing mechanism and the mixing tank and reduce the amount of gas dissolved in the degassed solution during the transmission process. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0052] Figure 1 A flow chart of the electrolyte ion concentration measurement method provided by the present invention;

[0053] Figure 2 A flow chart of another embodiment of the method for measuring electrolyte ion concentration provided by the present invention;

[0054] Figure 3 This is a schematic diagram of the structure of the electrolyte analysis device provided by the present invention;

[0055] Figure 4 A schematic diagram of the structure of the mixing tank provided by the present invention;

[0056] Figure 5 A schematic diagram of the structure of the mixing chamber provided by the present invention;

[0057] Figure 6 This is a schematic diagram of the structure of the stirring head provided by the present invention;

[0058] Figure 7 A cross-sectional view of the stirring head provided by the present invention;

[0059] Figure 8 This is a schematic structural diagram of the degassing module provided by the present invention.

[0060] Figure 1-Figure 8 middle:

[0061] 1. Mixing tank; 11. Filling port; 12. Liquid collecting tank; 13. Flow channel;

[0062] 2. Electrode assembly;

[0063] 3. Solenoid valve group; 31. First solenoid reversing valve; 32. Second solenoid reversing valve; 33. Third solenoid reversing valve; 34. Solenoid valve;

[0064] 4. Syringe;

[0065] 5. Degassing module; 51. First degassing module; 511. Liquid inlet hole; 512. Liquid discharge hole; 513. Degassing cavity; 514. Degassing membrane; 515. Negative pressure hole; 52. Second degassing module; 53. Pressure sensor;

[0066] 6. Degassing pump;

[0067] 7. Control module;

[0068] 8. stirring mechanism; 81. stirring head; 82. magnet; 83. rotating shaft; 84. stirring motor. DETAILED DESCRIPTION

[0069] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0070] The core of the present invention is to provide a method for measuring electrolyte ion concentration, by introducing an internal standard solution, comparing and calculating the measurement results of the sample solution with the measurement results of the internal standard solution, thereby eliminating the influence of temperature fluctuations on the final result.

[0071] Another core of the present invention is to provide an electrolyte analysis device for implementing the above-mentioned electrolyte ion concentration measurement method, eliminating the constant temperature module, simplifying the system, and adding a degassing module, further reducing the impact of the gas content in the solution on the effectiveness of the final result.

[0072] Please refer to Figure 1-Figure 2 , a method for measuring electrolyte ion concentration, comprising:

[0073] Measure the potential value Eb of the mixture of internal standard solution and buffer solution,

[0074] The potential value Els of the mixture of low-value calibration solution and buffer solution,

[0075] The potential value Ehs of the mixture of high value calibration solution and buffer solution,

[0076] The potential value Es of the mixture of sample solution and buffer solution;

[0077] The electrolyte concentration Cs of the sample solution is obtained according to the nominal concentration Ch of the high-value calibration solution, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the internal standard solution and the buffer solution, the potential value Els of the low-value calibration solution and the buffer solution, the potential value Ehs of the high-value calibration solution and the buffer solution, and the potential value Es of the sample solution and the buffer solution.

[0078] like Figure 1 As shown, by measuring the potential value Eb of the internal standard solution and the buffer solution, the potential value Els of the low value calibration solution and the buffer solution, the potential value Ehs of the high value calibration solution and the buffer solution, and the potential value Es of the sample solution and the buffer solution, and combining the existing data of the nominal concentration Ch of the high value calibration solution and the nominal concentration Cl of the low value calibration solution, and according to the formula:

[0079]

[0080] The electrolyte concentration Cs of the sample solution is obtained.

[0081] During the process, the potential value of the mixture of the internal standard solution and the buffer solution is used as a reference, and the potential values ​​of the low-value calibration solution and the high-value calibration solution and the buffer solution are measured respectively, and the potential difference between the low-value calibration solution and the high-value calibration solution and the internal standard solution is obtained, and then the calibration slope value SLOP is calculated;

[0082] The potential value of the sample solution and the buffer solution mixture is measured, and the potential difference between the sample solution and the internal standard solution is brought into the calculation to obtain the electrolyte concentration of the sample solution, thereby eliminating the influence of temperature fluctuations on the validity of the final result.

[0083] In some embodiments, the electrolyte concentration Cs of the sample solution is obtained according to the nominal concentration Ch of the high value calibration solution, the nominal concentration Cl of the low value calibration solution, the potential value Eb of the internal standard solution and the buffer solution, the potential value Els of the low value calibration solution and the buffer solution, the potential value Ehs of the high value calibration solution and the buffer solution, and the potential value Es of the sample solution and the buffer solution, further comprising:

[0084] The calibration slope value SLOP is obtained according to the potential value Eb of the mixture of the internal standard solution and the buffer solution, the potential value Els of the mixture of the low-value calibration solution and the buffer solution, and the potential value Ehs of the mixture of the high-value calibration solution and the buffer solution;

[0085] According to the calibration slope value SLOP, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the mixture of the internal standard solution and the buffer solution, and the potential value Els of the mixture of the low-value calibration solution and the buffer solution, the actual concentration CM1 of the internal standard solution is obtained;

[0086] The electrolyte concentration Cs of the sample solution is obtained according to the potential value Eb of the mixture of the internal standard solution and the buffer solution, the potential value Es of the mixture of the sample solution and the buffer solution, the actual concentration CM1 of the internal standard solution and the calibration slope value SLOP.

[0087] Specifically according to the formula:

[0088]

[0089] The electrolyte concentration Cs of the sample solution is obtained.

[0090] During the process, the potential value of the mixture of the internal standard solution and the buffer solution is used as a reference, and the potential values ​​of the low-value calibration solution, the high-value calibration solution and the buffer solution are measured respectively, and the potential difference between the low-value calibration solution, the high-value calibration solution and the internal standard solution is obtained, and then the calibration slope value SLOP is calculated, and the actual electrolyte concentration CM1 of the internal standard solution is calculated based on the potential value of the low-value calibration solution and the calibration slope value SLOP, thereby eliminating the influence of the error between the nominal concentration and the actual concentration of the internal standard solution on the validity of the final result.

[0091] In some embodiments, Figure 2 As shown, it also includes,

[0092] The solution factor d is obtained according to the potential value Eb of the mixture of the internal standard solution and the buffer solution, the potential value Els of the mixture of the low-value calibration solution and the buffer solution, the potential value Ehs of the mixture of the high-value calibration solution and the buffer solution, the nominal concentration Ch of the high-value calibration solution, the nominal concentration Cl of the low-value calibration solution and the nominal concentration CM of the internal standard solution;

[0093] The effectiveness of the electrolyte concentration Cs of the sample solution is determined based on the value of the solution factor d.

[0094] During the process, according to the formula:

[0095]

[0096] Get the solution factor d;

[0097] like , then the value of Cs is judged to be valid, otherwise, the value of Cs is judged to be invalid.

[0098] Before the test, the actual concentration and nominal concentration of the internal standard solution are first calculated in proportion. When the ratio of the actual concentration of the calibration solution to the nominal concentration exceeds the set range, it indicates that there is a large error in the reagent preparation process, and the validity of the final measurement result cannot be guaranteed. Therefore, the measurement result must be deemed invalid, thereby eliminating the problem of loss of validity of the final result caused by the large error between the nominal concentration and the actual concentration of the internal standard solution.

[0099] like Figure 3-Figure 8 As shown, in addition to the electrolyte ion concentration measurement methods disclosed in the above embodiments, the present invention also provides an electrolyte analysis device for implementing the above electrolyte ion concentration measurement method, including:

[0100] The mixing tank 1 includes a mixing chamber, a liquid inlet and a liquid discharge port connected to each other. The liquid inlet is provided with at least two liquid injection mechanisms, and the liquid discharge port is provided with at least one liquid discharge mechanism. The pipe for the sample liquid to flow in the liquid discharge mechanism is connected in series with an electrode assembly 2 for measuring the potential value of the sample liquid.

[0101] A stirring mechanism 8 is disposed in the mixing chamber and is used to stir the sample liquid in the mixing chamber;

[0102] The degassing module 5 is arranged at the inlet end of the liquid injection mechanism and is used to separate and remove the gas in the liquid in the liquid injection mechanism.

[0103] like Figure 3 As shown, a mixing tank 1 is used as a container for uniformly mixing multiple solutions, and an electrode assembly 2 is arranged in a pipe of a discharge mechanism. When the sample liquid is discharged from the mixing tank 1, it can flow through the electrode assembly 2, and then the potential value of the sample liquid is measured, wherein the electrode assembly 2 includes a ground plate, a C1 electrode, a K electrode, a Na electrode and a Ref electrode arranged in sequence, wherein the C1 electrode, the K electrode, the Na electrode and the Ref electrode are electrically connected to a calculation unit in a control module 7 through an amplifier and an analog-to-digital conversion module, thereby satisfying the potential value measurement of the sample liquid.

[0104] Meanwhile, a stirring mechanism 8 is integrated in the mixing tank 1 to stir the sample liquid entering the mixing tank 1 so as to fully blend the multiple sample liquids and avoid inaccurate measurement results caused by single-point electrolyte aggregation.

[0105] Moreover, a degassing module 5 is integrated at the inlet end of the liquid injection mechanism to degas the sample liquid that enters the mixing tank 1 in advance, so as to prevent the dissolved gas components in the sample liquid from affecting the validity of the final test calculation results. At the same time, arranging the degassing module 5 at the inlet end of the liquid injection mechanism helps to shorten the length of the pipeline between the degassing module 5 and the mixing tank 1, so that the degassed sample liquid can quickly enter the mixing tank 1, reducing the amount of dissolved gas during its re-transmission process.

[0106] In some embodiments, a syringe 4 and a stepper motor are also included, wherein the syringe 4 is provided with a plurality of independent piston chambers, and the stepper motor is used to drive the piston movement in the piston chamber;

[0107] The injection mechanism and the discharge mechanism both include a two-position three-way electromagnetic reversing valve, the piston chamber and the common end interface of the electromagnetic reversing valve are connected one by one, the normally closed port of the electromagnetic reversing valve is connected to the mixing tank 1, and the normally open port of the electromagnetic reversing valve is used to connect the internal standard liquid supply device, the buffer solution supply device or the waste liquid storage device.

[0108] like Figure 3As shown, both the injection mechanism and the discharge mechanism use the negative pressure or positive pressure generated by the action of the piston in the syringe 4 in the piston cavity as the power source, and the piston power of the syringe 4 comes from the drive of the stepper motor, and the stepper motor is controlled by the control module 7. The control module 7 is preferably an ISE control board, which integrates a single-chip microcomputer, a position sensor and a liquid level sensor. It can monitor the current position of the piston and the current volume of the sample liquid in the mixing tank 1 in real time, thereby ensuring the effective control of the sample liquid filling amount in the mixing tank 1.

[0109] During the test, the working steps include:

[0110] The normally open ends of the first electromagnetic reversing valve 31, the second electromagnetic reversing valve 32 and the third electromagnetic reversing valve 33 are all turned on, the syringe 4 starts suction, and the internal standard solution and the buffer solution enter the syringe 4;

[0111] The normally closed ends of the first electromagnetic reversing valve 31 and the second electromagnetic reversing valve 32 are both connected, and the normally open end of the third electromagnetic reversing valve 33 is connected, and the syringe 4 is injected and discharged, and the internal standard solution and buffer solution in the syringe 4 enter the mixing chamber;

[0112] The stirring mechanism 8 is activated to stir and mix the internal standard solution and the buffer solution in the mixing chamber to form a mixed solution;

[0113] The normally open ends of the first electromagnetic reversing valve 31 and the second electromagnetic reversing valve 32 are both turned on, and the normally closed end of the third electromagnetic reversing valve 33 is turned on. The syringe 4 performs suction operation, and the mixed liquid in the mixing chamber enters the syringe 4 and passes through the electrode assembly 2 during the process to measure the potential value.

[0114] The normally open ends of the first electromagnetic reversing valve 31, the second electromagnetic reversing valve 32 and the third electromagnetic reversing valve 33 are all connected, the syringe 4 is injected and discharged, and the mixed liquid in the syringe 4 enters the storage device of the waste liquid;

[0115] The normally open ends of the first electromagnetic reversing valve 31, the second electromagnetic reversing valve 32 and the third electromagnetic reversing valve 33 are all turned on, the syringe 4 starts suction, and the internal standard solution and the buffer solution enter the syringe 4;

[0116] The normally closed end of the first electromagnetic reversing valve 31 is turned on, and the normally open ends of the second electromagnetic reversing valve 32 and the third electromagnetic reversing valve 33 are turned on, the syringe 4 is injected and discharged, and the buffer solution in the syringe 4 enters the mixing chamber;

[0117] Use a pipette to add the sample liquid into the mixing chamber through the filling port 11 of the mixing tank 1, and the stirring mechanism 8 is activated to stir and mix the sample liquid and the buffer solution in the mixing chamber to form a sample mixed liquid;

[0118] The normally open ends of the first electromagnetic reversing valve 31 and the second electromagnetic reversing valve 32 are both turned on, and the normally closed end of the third electromagnetic reversing valve 33 is turned on. The syringe 4 performs suction operation, and the sample mixed liquid in the mixing chamber enters the syringe 4 and passes through the electrode assembly 2 during the process to measure the potential value.

[0119] The normally open ends of the first electromagnetic reversing valve 31 , the second electromagnetic reversing valve 32 and the third electromagnetic reversing valve 33 are all connected, the syringe 4 is injected and discharged, and the sample mixed liquid in the syringe 4 enters the waste liquid storage device.

[0120] The potential value measurement of the internal standard solution and the buffer solution mixture and the potential value measurement of the sample solution and the buffer solution mixture are then completed, and the electrolyte concentration Cs of the sample solution can be calculated.

[0121] During the process, since a plurality of piston chambers are provided in the syringe 4, which are respectively connected to the common ends of the first electromagnetic reversing valve 31, the second electromagnetic reversing valve 32 and the third electromagnetic reversing valve 33, the sample liquid entering the syringe 4 through the common ends of the first electromagnetic reversing valve 31, the second electromagnetic reversing valve 32 and the third electromagnetic reversing valve 33 will be stored separately and will not be mixed with each other.

[0122] In some embodiments, the degassing module 5 includes a connected liquid inlet hole 511, a degassing cavity 513 and a liquid discharge hole 512, a negative pressure hole 515 is provided at the top of the degassing cavity 513, a degassing pump 6 is connected in series to the negative pressure hole 515, and a degassing membrane 514 is provided at one end of the degassing cavity 513 close to the liquid inlet hole 511.

[0123] like Figure 8 As shown, the degassing module 5 adopts an internal degassing membrane 514 to degas the sample liquid flowing through, effectively reducing the gas content in the sample liquid, wherein the degassing membrane 514 adopts hydrophobic hollow fibers to effectively increase the actual working area and improve the degassing efficiency, and a negative pressure hole 515 is arranged on the top of the degassing chamber 513, and the negative pressure hole 515 is connected to the degassing pump 6 through the solenoid valve 34, and at the same time, a pressure sensor 53 is integrated in the degassing chamber 513 to detect the pressure in the degassing chamber 513. When the pressure is lower than the set range, the degassing pump 6 is started and the solenoid valve 34 is turned on, so that the pressure in the degassing chamber 513 is maintained within the set range for a long time, thereby ensuring the degassing effect of the sample liquid in the degassing chamber 513.

[0124] The solenoid valve 34 , the first solenoid reversing valve 31 , the second solenoid reversing valve 32 and the third solenoid reversing valve 33 together constitute a solenoid valve group 3 , which is directly electrically connected to the valve control unit in the control module 7 to achieve automatic control.

[0125] like Figure 1As shown, the degassing module 5 includes a first degassing module 51 and a second degassing module 52. The first degassing module 51 is arranged upstream of the first electromagnetic reversing valve 31, and the second degassing module 52 is arranged upstream of the second electromagnetic reversing valve 32. The first degassing module 51 and the second degassing module 52 share the same degassing pump 6, which helps to streamline the equipment.

[0126] In some embodiments, the mixing chamber is a circular cavity with a smooth inner wall;

[0127] The stirring mechanism 8 includes a stirring head 81 disposed in the mixing chamber; the stirring head 81 is a strip-shaped structure, and the end of the stirring head 81 is provided with a chamfer;

[0128] The stirring mechanism 8 further includes a stirring motor 84 disposed outside the mixing chamber, and a magnetic torque transmission mechanism is disposed between the output shaft end of the stirring motor 84 and the stirring head 81 .

[0129] like Figure 4 and Figure 5 As shown, the mixing chamber is set as a circular cavity, and the inner wall is kept smooth, which can reduce the residual amount of sample liquid on the inner wall of the mixing chamber. At the same time, the stirring head 81 can fully stir in the circular cavity to reduce the stirring dead angle;

[0130] like Figure 6 and Figure 7 As shown, the stirring head 81 is in the shape of an elongated strip, and a chamfer is set at the end to facilitate the sample liquid to pass quickly through the end of the stirring head 81 and reduce the stirring resistance. A magnet 82 is set inside the stirring head 81, and together with the auxiliary magnet at the end of the output shaft of the stirring motor 84, a magnetic torque transmission mechanism is formed, so that the stirring head 81 is inside the mixing chamber, and the stirring motor 84 is outside the mixing chamber. The two are not directly rigidly connected, but the torque is transmitted by magnetic force, so as to facilitate improving the sealing of the mixing chamber.

[0131] In some embodiments, a rotating shaft 83 is fixedly disposed at the rotation axis of the stirring head 81 , and the rotating shaft 83 is rotatably mounted with respect to the axis of the mixing chamber.

[0132] like Figure 6 and Figure 7 As shown, by setting a rotating shaft 83 in the stirring head 81 and rotatably installing it with the mixing chamber, the stirring head 81 is always in a set position, which is convenient for mutual attraction with the auxiliary magnet of the stirring motor 84, so that the stirring head 81 generates a rotational torque.

[0133] In some embodiments, an inverted conical liquid collecting trough 12 is disposed at the bottom of the mixing chamber, a downwardly inclined flow channel 13 is disposed between the liquid collecting trough 12 and the liquid discharge port, and the electrode assembly 2 is integrated in the flow channel 13 .

[0134] like Figure 5As shown, an inverted conical liquid collecting groove 12 is provided at the bottom of the mixing chamber. When the sample liquid in the mixing chamber is small, the sample liquid can be collected in the liquid collecting groove 12, and with the help of the downward inclined flow channel 13, a certain siphon effect can be generated during injection and discharge, thereby completely discharging the sample liquid in the mixing chamber and reducing the residual sample liquid in the mixing chamber.

[0135] During actual operation, in the early stage of injection and discharge, the syringe 4 is slowly aspirated to allow a large amount of sample liquid in the mixing chamber to enter the syringe 4. In the late stage of injection and discharge, after waiting for the sample liquid to accumulate in the liquid collecting tank 12, the syringe 4 is quickly aspirated to allow the liquid in the liquid collecting tank 12 to quickly enter the syringe 4, thereby reducing the residue in the mixing chamber.

[0136] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0137] The electrolyte ion concentration measurement method and analysis equipment provided by the present invention are introduced in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the present invention, the present invention can also be improved and modified, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A method for measuring electrolyte ion concentration, characterized in that: include: Measure the potential value Eb of the mixture of internal standard solution and buffer solution, The potential value Els of the mixture of low-value calibration solution and buffer solution, The potential value Ehs of the mixture of high value calibration solution and buffer solution, The potential value Es of the mixture of sample solution and buffer solution; The electrolyte concentration Cs of the sample solution is obtained according to the nominal concentration Ch of the high-value calibration solution, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the internal standard solution and buffer solution, the potential value Els of the low-value calibration solution and buffer solution, the potential value Ehs of the high-value calibration solution and buffer solution, and the potential value Es of the sample solution and buffer solution.

2. The method for measuring electrolyte ion concentration according to claim 1, characterized in that: The electrolyte concentration Cs of the sample solution is obtained according to the nominal concentration Ch of the high-value calibration solution, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the internal standard solution and the buffer solution, the potential value Els of the low-value calibration solution and the buffer solution, the potential value Ehs of the high-value calibration solution and the buffer solution, and the potential value Es of the sample solution and the buffer solution, further comprising: According to the potential value Eb of the internal standard solution and the buffer solution mixture, the potential value Els of the low-value calibration solution and the buffer solution mixture, and the potential value Ehs of the high-value calibration solution and the buffer solution mixture, a calibration slope value SLOP is obtained; According to the calibration slope value SLOP, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the internal standard solution and the buffer mixture, and the potential value Els of the low-value calibration solution and the buffer mixture, the actual concentration CM1 of the internal standard solution is obtained; The electrolyte concentration Cs of the sample solution is obtained according to the potential value Eb of the mixture of the internal standard solution and the buffer solution, the potential value Es of the mixture of the sample solution and the buffer solution, the actual concentration CM1 of the internal standard solution and the calibration slope value SLOP.

3. The method for measuring electrolyte ion concentration according to claim 2, characterized in that: According to the potential value Eb of the internal standard solution and the buffer solution mixture, the potential value Els of the low-value calibration solution and the buffer solution mixture, and the potential value Ehs of the high-value calibration solution and the buffer solution mixture, a calibration slope value SLOP is obtained; According to the calibration slope value SLOP, the nominal concentration Cl of the low-value calibration solution, the potential value Eb of the internal standard solution and the buffer mixture, and the potential value Els of the low-value calibration solution and the buffer mixture, the actual concentration CM1 of the internal standard solution is obtained; According to the potential value Eb of the mixture of the internal standard solution and the buffer solution, the potential value Es of the mixture of the sample solution and the buffer solution, the actual concentration CM1 of the internal standard solution and the calibration slope value SLOP, the electrolyte concentration Cs of the sample solution is obtained, further comprising: According to the formula: The electrolyte concentration Cs of the sample solution is obtained.

4. The method for measuring electrolyte ion concentration according to claim 1, characterized in that: Also includes, According to the potential value Eb of the mixture of the internal standard solution and the buffer solution, the potential value Els of the mixture of the low-value calibration solution and the buffer solution, the potential value Ehs of the mixture of the high-value calibration solution and the buffer solution, the nominal concentration Ch of the high-value calibration solution, the nominal concentration Cl of the low-value calibration solution and the nominal concentration CM of the internal standard solution, a solution factor d is obtained; The validity of the electrolyte concentration Cs of the sample solution is determined according to the value of the solution factor d.

5. The method for measuring electrolyte ion concentration according to claim 4, characterized in that: Judging the validity of the electrolyte concentration Cs of the sample solution according to the value of the solution factor d, further comprising: According to the formula: Obtaining the solution factor d; like , then the value of Cs is judged to be valid, otherwise, the value of Cs is judged to be invalid.

6. An electrolyte analysis device, characterized in that: The method for measuring electrolyte ion concentration according to any one of claims 1 to 5 comprises: A mixing pool (1) comprises a mixing chamber, a liquid inlet and a liquid discharge port which are connected to each other, wherein at least two groups of liquid injection mechanisms are arranged at the liquid inlet, and at least one group of liquid discharge mechanisms are arranged at the liquid discharge port, wherein a pipe in the liquid discharge mechanism for circulating a sample liquid is connected in series with an electrode assembly (2) for measuring the potential value of the sample liquid; A stirring mechanism (8), disposed in the mixing chamber, and used for stirring the sample liquid in the mixing chamber; A degassing module (5) is arranged at the inlet end of the liquid injection mechanism and is used to separate and remove gas from the liquid in the liquid injection mechanism.

7. The electrolyte analysis device according to claim 6, characterized in that: It also includes a syringe (4) and a stepping motor, wherein a plurality of independent piston chambers are arranged in the syringe (4), and the stepping motor is used to drive the pistons in the piston chambers to move; The injection mechanism and the discharge mechanism both comprise a two-position three-way electromagnetic reversing valve, the piston chamber is connected to the common end interface of the electromagnetic reversing valve in a one-to-one correspondence, the long-closed port of the electromagnetic reversing valve is connected to the mixing tank (1), and the normally open port of the electromagnetic reversing valve is used to connect the internal standard solution supply device, the buffer solution supply device or the waste liquid storage device.

8. The electrolyte analysis device according to claim 6, characterized in that: The degassing module (5) comprises a liquid inlet hole (511), a degassing cavity (513) and a liquid discharge hole (512) which are connected to each other, the liquid discharge hole (512) being connected to the liquid injection mechanism, and the liquid inlet hole being connected to the solution supply device; A negative pressure hole (515) is provided at the top of the degassing cavity (513), a degassing pump (6) is connected in series to the negative pressure hole (515), and a degassing membrane (514) is provided at one end of the degassing cavity (513) close to the liquid inlet hole (511).

9. The electrolyte analysis device according to claim 6, characterized in that: The mixing cavity is a circular cavity with a smooth inner wall; The stirring mechanism (8) comprises a stirring head (81) arranged in the mixing chamber; the stirring head (81) is a strip-shaped structure, and the end of the stirring head (81) is provided with a chamfer; The stirring mechanism (8) further comprises a stirring motor (84) arranged outside the mixing chamber, and a magnetic torque transmission mechanism is arranged between the output shaft end of the stirring motor (84) and the stirring head (81).

10. The electrolyte analysis device according to claim 9, characterized in that: A rotating shaft (83) is fixedly arranged at the rotation axis of the stirring head (81), and the rotating shaft (83) is rotatably mounted at the axis position of the mixing chamber.

11. The electrolyte analysis device according to any one of claims 6 to 10, characterized in that: An inverted conical liquid collecting trough (12) is provided at the bottom of the mixing chamber, a downwardly inclined flow channel (13) is provided in communication between the liquid collecting trough (12) and the liquid discharge port, and the electrode assembly (2) is integrated in the flow channel (13).

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