High-quality trichogramma breeding method

Abstract

The invention discloses a high-quality trichogramma breeding method, and relates to the technical field of trichogramma breeding, and the method comprises the following steps: host selection: selecting healthy, disease-free, moderate-size and convenient-to-parasitize corcyra cephalonica eggs as hosts; collection and preservation of host eggs: obtaining eggs from healthy host adults, disinfecting the eggs with ultraviolet rays or alcohol to reduce pathogens on the eggs, and preserving the eggs at low temperature after disinfection; inoculation of trichogramma: the mixing ratio of trichogramma to host eggs is 1: 10-1: 20, and the inoculation time is 24-48 hours to ensure full parasitism. According to the invention, the intelligent temperature and humidity recorder is used for monitoring and recording temperature and humidity data in the environment in real time, storing the data in the internal memory, and connecting the data to the PC after the recording function is completed, so that the temperature and humidity data can be checked in time and adjusted remotely in time, and red light is irradiated during inoculation, so that the intelligent temperature and humidity recorder is convenient to use. The red light (620-750 nm) environment can improve the activity and metabolism of the trichogramma and promote the smooth development of the trichogramma from eggs to adults.

Description

Technical Field

[0001] The invention relates to the technical field of trichogrammatid breeding, and in particular to a high-quality trichogrammatid breeding method. Background Art

[0002] Trichogrammatid is a general term for insects of the genus Trichogrammatidae family in the order Hymenoptera. It is named after a pair of large red compound eyes. Trichogrammatid can parasitize the eggs and larvae of many pests, such as rice moth, diamondback moth, pine caterpillar, etc. When the eggs of Trichogrammatid hatch in the pest eggs, they will suck the nutrients in the pest eggs to grow, and eventually break out of the shell, causing the death of the pest eggs or larvae. This parasitic habit makes Trichogrammatid the natural enemy of these pests. The number of pests can be effectively controlled through biological control, so the cultivation of Trichogrammatid is very important.

[0003] However, the current breeding technology of trichogrammatids has certain limitations. The control of temperature and humidity has always been manually controlled, which makes it impossible to detect and regulate drastic changes in temperature and humidity in time, resulting in adverse effects on the breeding of trichogrammatids. Therefore, a high-quality trichogrammatid breeding method is urgently needed. Summary of the invention

[0004] The purpose of the present invention is to provide a high-quality trichogrammatid breeding method to solve the problem that the current trichogrammatid breeding technology has certain limitations, and the control of temperature and humidity has always been manually controlled, so that when the temperature and humidity change drastically, it cannot be discovered in time, and cannot be adjusted in time, resulting in an adverse effect on the breeding of trichogrammatids.

[0005] To achieve the above object, the present invention provides the following technical solution: a method for cultivating high-quality trichogrammatids, comprising: Host selection: Select healthy, disease-free, moderately sized and easy-to-parasitize rice moth eggs as hosts; Collection and preservation of host eggs: obtain eggs from healthy host adults, sterilize with ultraviolet light or alcohol to reduce pathogens on the eggs, and store at low temperatures after sterilization; Inoculation of trichogrammatid: The mixing ratio of trichogrammatid to host eggs is 1:10-1:20, and the inoculation time is 24-48 hours to ensure full parasitism; Cultivation and management: Maintain appropriate temperature and humidity, provide appropriate light, simulate natural conditions, regularly check the parasitic conditions, adjust the parasitic environment in time, and optimize the light cycle; Collection of parasitic eggs: When the parasitic eggs turn black, the trichogrammatids are about to emerge, and collection can begin at this time; Adult emergence: 5-7 days after parasitism, maintain stable temperature and humidity during the emergence process to avoid drastic fluctuations, and provide honey water after emergence; Field release: Before release, spray green muscardine to enhance the control of pest larvae, release the pests in the egg stage to ensure that the trichogrammatids can find a host, avoid rainy or windy days, and choose a clear, windless morning or evening.

[0006] Preferably, the rice moth eggs are selected from multiple aspects including population, collection method, appearance, size, vitality, handling and storage.

[0007] Preferably, when collecting the rice moth, ensure that its population is healthy and disease-free, and that the eggs are naturally laid, and the rice moth eggs are disinfected, screened and preserved.

[0008] Preferably, the Trichogramma inoculation temperature is maintained at 25-28°C, an appropriate light cycle of 12 hours of light and 12 hours of darkness is provided, and the rice moth eggs are spread in a culture dish in the incubator for inoculation.

[0009] Preferably, in the cultivation management, an intelligent temperature and humidity recorder is used based on the Internet of Things technology to monitor the temperature and humidity in real time, and the temperature and humidity of the incubator are automatically adjusted to the optimal range (±1°C error).

[0010] Preferably, in the cultivation management, red light is irradiated during inoculation, and the red light (620-750nm) environment can improve the activity and metabolism of Trichogramma and shorten the development cycle of Trichogramma.

[0011] Preferably, the eclosion conditions require a temperature of 25-28°C, a humidity of 70-80%, and appropriate light.

[0012] Preferably, the release is done on rainy or windy days, and in the clear, windless morning or evening. Depending on the pest density, 10,000 to 20,000 pests are usually released per acre and spread evenly in the field to ensure full coverage.

[0013] Preferably, the Metarhizium muscardine is an insect pathogenic fungus that infects the body surface of pests and causes their death. It is effective against a variety of pests and is applied a few days before the release of the Trichogrammatids to ensure that it works without affecting the Trichogrammatids.

[0014] Compared with the prior art, the present invention has the following beneficial effects: In the present invention, the intelligent temperature and humidity recorder is used to monitor and record the temperature and humidity data in the environment in real time, and store the data in the internal memory. After completing the recording function, it is connected to the PC to facilitate timely viewing of the temperature and humidity data and remote adjustment. Red light (620-750nm) is irradiated during inoculation. The red light environment can improve the activity and metabolism of the trichogrammatids and promote their smooth development from eggs to adults.

[0015] In the present invention, trichogrammatids are often used to prevent and control corn borers, cotton bollworms, etc., and Metarhizium is an insect pathogenic fungus that infects the body surface of pests to cause their death, and is effective against a variety of pests. Trichogrammatids target eggs, and Metarhizium targets larvae and adults, forming a double control. Metarhizium weakens the pest population and enhances the parasitic effect of trichogrammatids; Metarhizium should be applied a few days before the release of trichogrammatids to ensure that it works without affecting the trichogrammatids. Spraying Metarhizium on trichogrammatids can reduce the use of chemical pesticides, reduce environmental pollution, and control pests for a long time and delay drug resistance. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the method flow of the present invention. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] See also Figure 1 , a high-quality trichogrammatid breeding method, comprising: Host selection: Select healthy, disease-free, moderately sized and easy-to-parasitize rice moth eggs as hosts; Collection and preservation of host eggs: obtain eggs from healthy host adults, sterilize with ultraviolet light or alcohol to reduce pathogens on the eggs, and store at low temperatures after sterilization; Inoculation of trichogrammatid: The mixing ratio of trichogrammatid to host eggs is 1:10-1:20, and the inoculation time is 24-48 hours to ensure full parasitism; Cultivation and management: Maintain appropriate temperature and humidity, provide appropriate light, simulate natural conditions, regularly check the parasitic conditions, adjust the parasitic environment in time, and optimize the light cycle; Collection of parasitic eggs: When the parasitic eggs turn black, the trichogrammatids are about to emerge, and collection can begin at this time; Adult emergence: 5-7 days after parasitism, maintain stable temperature and humidity during the emergence process to avoid drastic fluctuations, and provide honey water after emergence; Field release: Before release, spray green muscardine to enhance the control of pest larvae, release the pests in the egg stage to ensure that the trichogrammatids can find a host, avoid rainy or windy days, and choose a clear, windless morning or evening.

[0019] Example 1 As a preferred embodiment of the present invention: the selection of rice moth eggs is based on population, collection method, appearance, size, vitality, processing and storage; Collection of rice moth eggs, population: obtain eggs from healthy and disease-free rice moth populations, ensure that the rice moth breeding environment is clean and avoid pathogens and parasites; collection method: let rice moths lay eggs naturally in a suitable environment, and induce rice moths to lay eggs in a concentrated manner by controlling light and temperature; appearance: healthy rice moth eggs are milky white or light yellow, with a smooth surface, plump eggs, uniform size, and no obvious deformation or damage; size: use a standard sieve to screen out eggs of uniform size, among which eggs with a diameter of 0.5-0.7 mm are most suitable for parasitism by trichogrammatids; vitality: randomly sample and test the hatching rate of eggs, select batches with high hatching rates, use a small amount of trichogrammatids for parasitism tests, and evaluate the parasitism rate of eggs; disinfection: irradiate eggs with ultraviolet rays to kill surface pathogens, storage conditions: the storage temperature is controlled at 4-10℃℃ to delay the development of eggs, and the relative humidity is maintained at 50-60% to prevent the eggs from drying out. The storage time should not be too long, usually not more than 7 days.

[0020] Example 2 As a preferred embodiment of the present invention: when collecting rice moths, ensure that their population is healthy, disease-free, and naturally spawned, and sterilize, screen and preserve the rice moth eggs; Egg-laying environment: Provide a suitable environment, such as suitable temperature (25-28℃) and humidity (70-80%), as well as sufficient light; Collection methods include natural egg-laying and artificial induction. Natural egg-laying: Let the rice moth lay eggs naturally in the egg-laying box. Egg-laying paper or gauze can be placed in the egg-laying box to facilitate the attachment and collection of eggs; Artificial induction: Induce rice moths to lay eggs in a concentrated manner by controlling light and temperature, such as placing rice moths in a dark environment and then allowing them to lay eggs under light conditions; Treatment of rice moths: Use ultraviolet rays to irradiate the eggs to kill surface pathogens. The irradiation time is usually 15-30 minutes. Use a standard sieve to screen out eggs with a diameter of Eggs of 0.5-0.7 mm in size should be removed, and those that are deformed, damaged or have abnormal colors should be removed to ensure that the eggs are healthy; Storage of rice moths: The storage temperature should be controlled at 4-10℃ to delay the development of eggs, and the relative humidity should be maintained at 50-60% to prevent the eggs from shrunken. The storage environment should be well ventilated to avoid the growth of mold. Use containers with good air permeability, such as gauze bags or perforated plastic boxes, to prevent the eggs from suffocating. Mark the collection date, batch and other information on the container for easy management and traceability. The storage time should not be too long, usually not more than 7 days, to ensure the vitality and parasitic rate of the eggs. Check the stored eggs regularly and remove the spoiled or moldy eggs in time.

[0021] Example 3 As a preferred embodiment of the present invention: the inoculation temperature of the trichogrammatid wasp is maintained at 25-28°C, an appropriate light cycle of 12 hours of light and 12 hours of darkness is provided, and the rice moth eggs are spread in a culture dish in the incubator for inoculation; Inoculation environment: The temperature is maintained at 25-28℃, the humidity is maintained at 70-80%, and an appropriate light cycle is provided, usually 12 hours of light and 12 hours of darkness; Inoculation steps: Spread the rice moth eggs evenly in the culture dish, introduce the trichogrammatid wasp into the container containing the rice moth eggs, ensure that the bee and the eggs are in full contact, place the inoculated container in a controlled environment, wait for the trichogrammatid wasp to parasitize the rice moth eggs, check the parasitism regularly, and record the parasitism rate and the development progress of the trichogrammatid wasp.

[0022] Example 4 As a preferred embodiment of the present invention: in the cultivation management, an intelligent temperature and humidity recorder is used based on the Internet of Things technology to monitor the temperature and humidity in real time, and the temperature and humidity of the incubator are automatically adjusted to the optimal range (±1°C error); The smart temperature and humidity recorder is an intelligent device that integrates sensor technology, Internet of Things technology and data processing technology. It is mainly used to monitor and record the temperature and humidity data in the environment in real time. The temperature and humidity recorder measures the temperature and humidity parameters and stores them in the internal memory at predetermined time intervals. After completing the recording function, it is connected to the PC to facilitate timely viewing of temperature and humidity data and make timely adjustments.

[0023] Example 5 As a preferred embodiment of the present invention: during cultivation management, red light is irradiated during inoculation, and the red light (620-750nm) environment can improve the activity and metabolism of trichogrammatids and shorten the development cycle of trichogrammatids; Red light is often used to regulate the diapause (a dormant state) of trichogrammatids. Through red light irradiation, diapause can be broken or induced, helping trichogrammatids to develop normally under specific conditions. Red light regulates the metabolism and hormone levels of trichogrammatids, promoting their smooth development from eggs to adults. Red light can affect the activity patterns of trichogrammatids, such as mating and egg-laying behaviors. In a red light environment, the activities of trichogrammatids may be inhibited, which is convenient for manual operation and management. Red light can also reduce the stress response of trichogrammatids, making them more stable in artificial environments. Red light irradiation operation: Use red light with a wavelength of 620-750nm to ensure uniform lighting. According to the developmental stage and needs of the trichogrammatids, it is usually irradiated for 12-16 hours a day. The specific time needs to be adjusted according to the experimental or production needs. The light intensity should be moderate to avoid excessive or weak light that affects the normal development of the trichogrammatids.

[0024] Example 6 As a preferred embodiment of the present invention: the eclosion conditions require a temperature of 25-28°C, a humidity of 70-80%, and appropriate light; The specific temperature is 25-28℃ during the day and 20-22℃ at night; the relative humidity is maintained at 70-80%, and a humidifier or spray system can be used to maintain stable humidity; provide 12-14 hours of light to simulate the natural light cycle, and the light intensity is controlled at 2000-3000 lux; provide sufficient space to avoid overcrowding, maintain good ventilation, and prevent carbon dioxide accumulation; clean the culture containers regularly to prevent mold and bacterial contamination, disinfect tools and containers, and reduce the spread of pathogens; and regularly observe the growth and reproduction of Trichogramma.

[0025] Example 7 As a preferred embodiment of the present invention: avoid rainy or windy days when releasing, choose a sunny, windless morning or evening, and according to the pest density, usually release 10,000 to 20,000 heads per mu, evenly spread in the field to ensure comprehensive coverage; Release time: choose clear, windless or breezy weather, avoid high temperatures, strong light or rainy days, the best time is early morning or evening; Release method: Mix Trichogramma eggs with a carrier (such as rice husks) and spread them evenly in the field; Set release points evenly according to the distribution of crops in the field and the occurrence of pests, usually 10 to 15 points per mu; After releasing the Trichogramma, try to avoid using broad-spectrum chemical pesticides to avoid killing the Trichogramma. If medication is necessary, choose biological pesticides or low-toxic pesticides that are safe for Trichogramma. Regularly check the parasitism rate of pest eggs and evaluate the control effect of Trichogramma. Generally, a parasitism rate of more than 70% is considered to be good. If the pest occurrence period is longer or the insect population density is higher, a second release can be carried out 7 to 10 days after the first release.

[0026] Example 8 As a preferred embodiment of the present invention: Metarhizium is an insect pathogenic fungus that infects the body surface of pests and causes their death. It is effective against a variety of pests and should be applied a few days before the release of trichogrammatids to ensure that it works without affecting the trichogrammatids. Trichogrammatis is an egg parasitic wasp that reduces the hatching rate of pests by parasitizing their eggs. It is often used to control corn borer, cotton bollworm, etc. Metarhizium is an insect pathogenic fungus that infects the pest's body surface and causes its death. It is effective against many pests. Trichogrammatis targets eggs, while Metarhizium targets larvae and adults, forming a double control. Metarhizium weakens the pest population and enhances the parasitic effect of Trichogrammatis. Metarhizium should be applied a few days before the release of Trichogrammatis to ensure that it works and does not affect the Trichogrammatis. Spraying Metarhizium on Trichogrammatis can reduce the use of chemical pesticides, reduce environmental pollution, and control pests for a long time and delay drug resistance.

[0027] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-restrictive, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations within the meaning and range of equivalents of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A high-quality trichogrammatid breeding method, characterized in that: include: Host selection: Select healthy, disease-free, moderately sized and easy-to-parasitize rice moth eggs as hosts; Collection and preservation of host eggs: obtain eggs from healthy host adults, sterilize with ultraviolet light or alcohol to reduce pathogens on the eggs, and store at low temperatures after sterilization; Inoculation of trichogrammatid: The mixing ratio of trichogrammatid to host eggs is 1:10-1:20, and the inoculation time is 24-48 hours to ensure full parasitism; Cultivation and management: Maintain appropriate temperature and humidity, provide appropriate light, simulate natural conditions, regularly check the parasitic conditions, adjust the parasitic environment in time, and optimize the light cycle; Collection of parasitic eggs: When the parasitic eggs turn black, the trichogrammatids are about to emerge, and collection can begin at this time; Adult emergence: 5-7 days after parasitism, maintain stable temperature and humidity during the emergence process to avoid drastic fluctuations, and provide honey water after emergence; Field release: Before release, spray green muscardine to enhance the control of pest larvae, release the pests in the egg stage to ensure that the trichogrammatids can find a host, avoid rainy or windy days, and choose a clear, windless morning or evening.

2. A high-quality trichogrammatid breeding method according to claim 1, characterized in that: The rice moth eggs are selected from multiple aspects including population, collection method, appearance, size, vitality, processing and storage.

3. A high-quality trichogrammatid breeding method according to claim 1, characterized in that: When the rice moth is collected, it is ensured that the population is healthy and free of diseases and that the eggs are laid naturally, and the rice moth eggs are disinfected, screened and preserved.

4. A high-quality trichogrammatid breeding method according to claim 1, characterized in that: The inoculation temperature of the trichogrammatid was maintained at 25-28° C., an appropriate light cycle of 12 hours of light and 12 hours of darkness was provided, and the rice moth eggs were spread in a culture dish in the incubator for inoculation.

5. A high-quality trichogrammatid breeding method according to claim 1, characterized in that: In the cultivation management, an intelligent temperature and humidity recorder is used based on the Internet of Things technology to monitor the temperature and humidity in real time, and automatically adjust the temperature and humidity of the incubator to the optimal range (±1°C error).

6. A high-quality trichogrammatid breeding method according to claim 1, characterized in that: In the cultivation management, red light is irradiated during inoculation, and the red light (620-750nm) environment can improve the activity and metabolism of trichogrammatids and shorten the development cycle of trichogrammatids.

7. A high-quality trichogrammatid breeding method according to claim 1, characterized in that: The requirements for the emergence conditions are a temperature of 25-28°C, a humidity of 70-80%, and appropriate light.

8. A high-quality trichogrammatid breeding method according to claim 1, characterized in that: The release is performed on rainy or windy days, and in the clear and windless morning or evening. Depending on the pest density, 10,000 to 20,000 insects are usually released per mu, and evenly spread in the field to ensure full coverage.

9. A high-quality trichogrammatid breeding method according to claim 1, characterized in that: The green muscardine is an insect pathogenic fungus that infects the body surface of pests and causes their death. It is effective against a variety of pests and is applied a few days before the release of trichogrammatids to ensure that it works without affecting the trichogrammatids.

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