Gas circulation system of cell incubator

Abstract

The invention belongs to the technical field of cell culture, and particularly relates to a cell culture box gas circulation system which comprises a gas supply cylinder, a gas inlet cylinder is fixed to one end of the gas supply cylinder, a gas outlet cylinder is fixed to the other end of the gas supply cylinder, and the ends, located in the gas supply cylinder, of the gas inlet cylinder and the gas outlet cylinder are sealed; a plurality of connecting air holes are formed in the positions, located in the air supply cylinder, of the outer walls of the air inlet cylinder and the air outlet cylinder. A fixed seat of a disc-shaped structure is fixed between the air inlet cylinder and the air outlet cylinder, the circumferential outer wall of the fixed seat is rotationally connected with a dispersion cylinder, the two ends of the dispersion cylinder are open, and a gap is reserved between the outer wall of the dispersion cylinder and the inner wall of the air supply cylinder; a plurality of air supply pipes are fixed to the end, close to the air inlet cylinder, of the outer wall of the air supply cylinder in a penetrating mode. When oxygen, carbon dioxide or nitrogen is supplemented by circulating gas, the supplemented gas is quickly and uniformly dispersed, so that the gas supply uniformity and the operation effect of cell culture are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and particularly to a gas circulation system for a cell culture incubator. Background Art

[0002] Cell culture mixed gas is one of the essential conditions for the survival of cells in vitro. The main components of the mixed gas are O2 (oxygen), CO2 (carbon dioxide gas), and N2 (nitrogen).

[0003] The Chinese invention patent with the application number 2023108937204 discloses an intake control method for a three-gas supply system of a cell culture incubator, which is applied to a cell culture incubator with three gas sources of CO2, O2, and N2. The intake control method for the three-gas supply system of the cell culture incubator of the present invention adopts a step-by-step adjustment strategy to give priority to ensuring the concentration of carbon dioxide gas. When the concentration of carbon dioxide gas is stable, in order to make the oxygen reach the set concentration and the concentration of carbon dioxide remain unchanged, the subsequent added gas always contains the carbon dioxide gas in this proportion; if the concentration of carbon dioxide is too high, nitrogen is preferentially filled to reduce its concentration to the set value. Through the above method, it is possible to achieve the adjustment of oxygen and carbon dioxide concentrations at any concentration and the requirements of high-precision control;

[0004] In the above patent and the prior art for the gas supply system of a cell culture incubator, generally, the gas in the cell culture incubator is circulated externally through a pipeline, and the corresponding amounts of oxygen, carbon dioxide, or nitrogen are introduced into the circulating gas according to requirements. However, in the prior art, the supplementary gas is often directly introduced along the flow direction of the circulating gas and flows back into the cell culture incubator before being evenly dispersed in time, which easily causes differences in the local area of the cell culture incubator during the supplementary gas supply and affects the cell culture effect. Summary of the Invention

[0005] Based on the technical problems in the background art, the present invention proposes a gas circulation system for a cell culture incubator.

[0006] A gas circulation system for a cell culture incubator proposed by the present invention includes a gas supply cylinder. One end of the gas supply cylinder is fixed with an intake cylinder, and the other end of the gas supply cylinder is fixed with an exhaust cylinder. The ends of the intake cylinder and the exhaust cylinder located inside the gas supply cylinder are blocked. A plurality of connecting air holes are opened at the positions of the outer walls of the intake cylinder and the exhaust cylinder located inside the gas supply cylinder; A fixed seat with a disc-shaped structure is fixed between the intake cylinder and the exhaust cylinder. The circumferential outer wall of the fixed seat is rotatably connected with a dispersion cylinder. Both ends of the dispersion cylinder are open, and a gap is left between the outer wall of the dispersion cylinder and the inner wall of the gas supply cylinder; A plurality of gas supply pipes are fixedly penetrated through one end of the outer wall of the gas supply cylinder close to the intake cylinder.

[0007] Preferably, a connecting gear ring is fixed at one end of the inner wall of the dispersion cylinder close to the air inlet cylinder. One end of the inner wall of the air supply cylinder corresponding to the connecting gear ring is rotatably connected through a bearing to a horizontally placed driving shaft. A driving gear meshing with the connecting gear ring is fixed at one end of the driving shaft. The other end of the driving shaft is drivingly connected to a driving motor. The outer wall of the dispersion cylinder is provided with dispersion grooves distributed at intervals and staggered.

[0008] Preferably, a spirally distributed air supply blade I is fixed at a position of the inner wall of the dispersion cylinder corresponding to the air inlet cylinder, and a spirally distributed air supply blade II is fixed at a position of the inner wall of the dispersion cylinder corresponding to the air outlet cylinder. Both the air supply blade I and the air supply blade II send air towards the air inlet cylinder direction as the dispersion cylinder rotates; the outer diameters of the air inlet cylinder and the air outlet cylinder inside the dispersion cylinder gradually decrease towards the fixed seat direction, and the width of the air supply blade I is greater than the width of the air supply blade II.

[0009] Preferably, a plurality of connecting pipes are horizontally and fixedly penetrated inside between the air inlet cylinder and the air outlet cylinder. One end of the connecting pipe close to the air inlet cylinder is communicated with a flexible pipe, and one end of the flexible pipe is communicated with an air bag. An installation groove is opened at a position of the outer wall of the air inlet cylinder corresponding to the air supply pipe, and the air bag is fixed to the inner wall of the installation groove; a limiting ring is arranged at one end of the connecting pipe away from the flexible pipe, and an extension rod is slidably connected to the inner wall of the limiting ring. A piston slidably connected to the inner wall of the connecting pipe is fixed at one end of the extension rod. A first spring is connected between the piston and the limiting ring. A baffle located inside the air outlet cylinder is fixed at one end of the extension rod away from the piston.

[0010] Preferably, a plurality of dispersion components are arranged at the position between the outer wall of the dispersion cylinder and the inner wall of the air supply cylinder. The dispersion component is provided with a horizontally extending positioning rod. Two mounting blocks are slidably connected to the outer wall of the positioning rod, and the mounting blocks are fixedly connected to the inner wall of the air supply cylinder. Two third springs are fixed at the position between the two mounting blocks on the outer wall of the positioning rod. One end of each of the two third springs away from each other abuts against the mounting block; a plurality of mounting holes are opened on the outer wall of the positioning rod, and a positioning block is rotatably connected to the inner wall of the mounting hole through a torsion spring. An air flow blade is fixed at one end of the positioning block, and the air flow blade is inclined with respect to the axis of the positioning rod.

[0011] Preferably, one end of the air supply pipe facing the inner wall of the air supply cylinder is rotatably connected through a bearing to an extension pipe. A plurality of through holes are opened at a position of the outer wall of the extension pipe corresponding to the opening of the dispersion cylinder. An air groove is opened on the outer wall of the extension pipe and is distributed at intervals with the through holes, and the air groove is inclined.

[0012] Preferably, an embedding groove is opened at a position of the outer wall of the extension pipe corresponding to the positioning rod, and a first magnetic block is fixed to the inner wall of the embedding groove. A second magnetic attracting block is fixed at one end of the positioning rod close to the extension pipe.

[0013] Preferably, a plurality of through holes are formed in the side wall of the fixed seat. A connecting rod is slidably connected to the inner wall of the through hole. Both ends of the connecting rod are fixed with movable members in a fan shape. Elastic cloth is connected between adjacent two movable members, and between the movable members and the outer walls of the air inlet cylinder and the air outlet cylinder. Air flow grooves are formed in the circumferential periphery of the outer walls on both sides of the movable member.

[0014] Preferably, the movable member near the air inlet cylinder is set as the first movable plate, and the movable member near the air outlet cylinder is set as the second movable plate. A second spring is connected between the second movable plate and the fixed seat. The outer diameter of the second movable plate is larger than that of the first movable plate.

[0015] Preferably, auxiliary grooves are formed at the edge positions of the side walls of the movable members close to the dispersion cylinder. Second magnetic blocks are fixed to the inner walls of the auxiliary grooves. First magnetic attraction blocks are fixed to the positions of the outer wall of the positioning rod corresponding to the movable members. The two first magnetic attraction blocks are symmetrically distributed with respect to the positioning rod.

[0016] The beneficial effects in the present invention are as follows:

[0017] 1. In the embodiment of the present invention, by slowing down the speed of the air flow entering the air inlet cylinder and contacting the directly introduced supplementary gas, the directly introduced supplementary gas can be quickly dispersed into the slowed-down air inlet air, so that the gas is quickly and evenly dispersed, and enters the dispersion cylinder from the other end opening of the dispersion cylinder, and enters through the connection air holes outside the air outlet cylinder, and circulates back to the cell culture box along the air outlet cylinder; thus, when circulating gas is used to supplement oxygen, carbon dioxide or nitrogen, the supplementary gas is quickly and evenly dispersed, so as to improve the gas supply uniformity and the operation effect of cell culture.

[0018] 2. In the embodiment of the present invention, by the width difference between the first air supply blade and the second air supply blade in cooperation with the change of the outer diameters of the air inlet cylinder and the air outlet cylinder, the air flow circulates at the connection air hole positions outside the air inlet cylinder and the air outlet cylinder, so as to further improve the uniform dispersion of the gas circulating back to the cell culture box.

[0019] 3. In the embodiment of the present invention, the supplementary gas supplied is dispersed around by the air bag deformed by the gas, and the air bag squeezes the piston and the extension rod to move towards the air outlet cylinder, so that the baffle at the corresponding position moves towards the connection air hole of the air outlet cylinder, so that the staggered baffles further disperse the outflowing air flow when the gas is supplied, and further improve the uniform mixing efficiency of the circulating air flow.

[0020] 4. In the embodiment of the present invention, through the arranged magnetic blocks and magnetic attraction blocks, the air flow movement when the supplementary gas is supplied by the gas supply pipe, the air flow in the two side regions of the fixed seat, and the air flow at the dispersion component position are cooperatively guided and dispersed, so as to avoid the air flow moving in a group due to always moving evenly, thereby further improving the uniformity of the dispersion of the gas supplied to the circulating air flow and the working efficiency of uniform dispersion. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 FIG. 1 is a schematic diagram of the overall structure of a gas circulation system for a cell incubator proposed by the present invention;

[0022] Figure 2 FIG. 2 is a schematic diagram of the internal structure of the air supply cylinder of a gas circulation system for a cell incubator proposed by the present invention;

[0023] Figure 3 FIG. 3 is a schematic diagram of the internal structure of the dispersion cylinder of a gas circulation system for a cell incubator proposed by the present invention;

[0024] Figure 4 FIG. 4 is a schematic diagram of the distribution structure of the first air supply blade and the second air supply blade of a gas circulation system for a cell incubator proposed by the present invention;

[0025] Figure 5 FIG. 5 is a schematic diagram of the connection gear ring structure of a gas circulation system for a cell incubator proposed by the present invention;

[0026] Figure 6 FIG. 6 is a schematic diagram of the extension pipe structure of a gas circulation system for a cell incubator proposed by the present invention;

[0027] Figure 7 FIG. 7 is a schematic diagram of the air inlet cylinder and the air outlet cylinder of a gas circulation system for a cell incubator proposed by the present invention;

[0028] Figure 8 FIG. 8 is a schematic diagram of the internal structure of the air inlet cylinder and the air outlet cylinder of a gas circulation system for a cell incubator proposed by the present invention;

[0029] Figure 9 FIG. 9 is a schematic diagram of the sectional structure of the connecting pipe of a gas circulation system for a cell incubator proposed by the present invention;

[0030] Figure 10 FIG. 10 is a schematic diagram of the fixed seat structure of a gas circulation system for a cell incubator proposed by the present invention;

[0031] Figure 11 FIG. 11 is a schematic diagram of the movable part structure of a gas circulation system for a cell incubator proposed by the present invention;

[0032] Figure 12 FIG. 12 is a schematic diagram of the dispersion component structure of a gas circulation system for a cell incubator proposed by the present invention;

[0033] Figure 13 FIG. 13 is a schematic diagram of the air flow blade structure of a gas circulation system for a cell incubator proposed by the present invention.

[0034] In the figure: 1 air supply cylinder, 2 intake cylinder, 3 outlet cylinder, 4 connecting air holes, 5 fixed seat, 501 limiting sliding groove, 6 dispersion cylinder, 601 slider, 602 dispersion groove, 7 air supply pipe, 8 connecting gear ring, 9 drive shaft, 10 drive motor, 11 drive gear, 12 air supply blade one, 13 air supply blade two, 14 extension pipe, 141 through hole, 142 air groove, 143 magnet one, 15 connecting pipe, 16 airbag, 17 hose, 18 piston, 19 extension rod, 20 baffle, 21 spring one, 22 moving part, 221 moving plate one, 222 moving plate two, 23 connecting rod, 24 spring two, 25 elastic cloth, 26 air flow groove, 27 magnet two, 28 dispersion assembly, 29 mounting block, 30 positioning rod, 31 positioning block, 32 air flow blade, 33 magnetic attracting block one, 34 magnetic attracting block two, 35 spring three. Detailed implementation mode

[0035] Example 1

[0036] Refer to Figures 1 - 5 , a gas circulation system for a cell culture incubator, includes an air supply cylinder 1. One end of the air supply cylinder 1 is fixed with an intake cylinder 2. The intake cylinder 2 is communicated with the outlet end of the cell culture incubator through an air pump. The other end of the air supply cylinder 1 is fixed with an outlet cylinder 3. The outlet cylinder 3 is communicated with the intake end of the cell culture incubator through an air pump. The ends of the intake cylinder 2 and the outlet cylinder 3 located inside the air supply cylinder 1 are blocked. Multiple connecting air holes 4 are opened at the positions of the outer walls of the intake cylinder 2 and the outlet cylinder 3 located inside the air supply cylinder 1; A fixed seat 5 with a disc-shaped structure is fixed between the intake cylinder 2 and the outlet cylinder 3. The circumferential outer wall of the fixed seat 5 is rotatably connected with a dispersion cylinder 6. A limiting sliding groove 501 is opened on the circumferential outer wall of the fixed seat 5. A slider 601 is fixed at the position of the inner wall of the dispersion cylinder 6 corresponding to the limiting sliding groove 501. The slider 601 is limited and slid between the limiting sliding groove 501. Both ends of the dispersion cylinder 6 are open. A gap is left between the outer wall of the dispersion cylinder 6 and the inner wall of the air supply cylinder 1; A plurality of air supply pipes 7 are penetrated and fixed at one end of the outer wall of the air supply cylinder 1 close to the intake cylinder 2. The air supply pipes 7 are arranged at the positions between the end of the dispersion cylinder 6 and the inner wall of the corresponding end of the air supply cylinder 1. Thus, the dispersion cylinder 6 is divided into two chambers corresponding to the positions of the intake cylinder 2 and the outlet cylinder 3 respectively by the blockage of the fixed seat 5. The gas flowing out of the cell culture incubator in the outward circulation enters the air supply cylinder 1 along the intake cylinder 2 and flows out through the connecting air holes 4 outside the intake cylinder 2. The entering gas is blocked and buffered by the fixed seat 5 in the intake direction, so that the entering gas flows reversely towards the position of the air supply pipe 7 to slow down the air flow entering speed;

[0037] The air supply pipes 7 at each position inject corresponding amounts of supplementary gas into the air supply cylinder 1 according to requirements. By slowing down the speed of the air flow entering the intake cylinder 2, the supplementary gas directly introduced comes into contact with the slowed-down intake air, so that the directly introduced supplementary gas is quickly dispersed into the slowed-down intake air, so that the gas is quickly and evenly dispersed;

[0038] Then the mixed gas flows along the gap between the outer casing of the dispersion cylinder 6 and the inside of the air supply cylinder 1 towards the air outlet cylinder 3, enters the dispersion cylinder 6 from the opening at the other end of the dispersion cylinder 6, enters through the connecting air holes 4 outside the air outlet cylinder 3, and circulates back to the cell incubator along the air outlet cylinder 3; thus, when the circulating gas replenishes oxygen, carbon dioxide or nitrogen, the replenishing gas is quickly and evenly dispersed, so as to improve the air supply uniformity and the operation effect of cell culture.

[0039] In the present invention, referring to Figures 1 - 5 , at one end of the inner wall of the dispersion cylinder 6 close to the air inlet cylinder 2, a connecting gear ring 8 is fixed. One end inner wall of the air supply cylinder 1 corresponding to the connecting gear ring 8 is rotationally connected through a bearing with a horizontally placed driving shaft 9. One end of the driving shaft 9 is fixed with a driving gear 11 meshing with the connecting gear ring 8, and the other end of the driving shaft 9 is drivingly connected with a driving motor 10. The outer wall of the dispersion cylinder 6 is provided with dispersion grooves 602 distributed at intervals and staggered, so as to continuously rotate the dispersion cylinder 6 by using the driving motor 10 during the process of circulating and flowing the gas and continuously replenishing the air supply, and cooperate with the dispersion grooves 602 dispersedly arranged on the outer wall of the dispersion cylinder 6 to disturb and disperse the airflow flowing outside the dispersion cylinder 6, so as to further improve the rapid and uniform dispersion of the mixed airflow.

[0040] In the present invention, referring to Figures 1 - 7 , at the position of the inner wall of the dispersion cylinder 6 corresponding to the air inlet cylinder 2, a spirally distributed air supply blade one 12 is fixed. At the position of the inner wall of the dispersion cylinder 6 corresponding to the air outlet cylinder 3, a spirally distributed air supply blade two 13 is fixed. Both the air supply blade one 12 and the air supply blade two 13 send air towards the air inlet cylinder 2 as the dispersion cylinder 6 rotates; the outer diameters of the air inlet cylinder 2 and the air outlet cylinder 3 inside the dispersion cylinder 6 gradually decrease towards the fixed seat 5. The width of the air supply blade one 12 is greater than the width of the air supply blade two 13, so that the inner side of the air supply blade one 12 is closer to the axis positions of the air inlet cylinder 2 and the air outlet cylinder 3 than the air supply blade two 13. By using the air supply blade one 12 that rotates with the dispersion cylinder 6, the incoming airflow is sent towards the position of the air supply pipe 7, and in cooperation with the change in the outer diameter of the air inlet cylinder 2, the airflow blown by the air supply pipe 7 flows along the outer wall of the air inlet cylinder 2 to form a circulating flow outside the air inlet cylinder 2, so that the supplied replenishing gas and the circulating airflow are quickly and evenly distributed;

[0041] At the position of the air outlet cylinder 3, part of the gas is sent towards the fixed seat 5 along the inner wall of the dispersion cylinder 6 through the air supply blade two 13 with a smaller width, and then flows towards the air outlet direction along the inclined outer wall of the air outlet cylinder 3; and because the width of the air supply blade two 13 is smaller, part of the airflow directly flows along the outer wall of the air outlet cylinder 3 towards the connecting air holes 4; and the two airflows collide and disperse at the position of the connecting air holes 4, so as to further improve the uniform dispersion of the gas circulating back to the cell incubator.

[0042] Example 2

[0043] Embodiment 2 includes all the structures and methods of Embodiment 1. Refer to Figures 1 - 9 , a gas circulation system for a cell incubator, further comprising a plurality of connecting pipes 15 horizontally and fixedly penetrating inside between the air inlet cylinder 2 and the air outlet cylinder 3. One end of the connecting pipe 15 close to the air inlet cylinder 2 is communicated with a flexible hose 17, and one end of the flexible hose 17 is communicated with an airbag. An installation groove is formed at a position on the outer wall of the air inlet cylinder 2 corresponding to the air supply pipe 7, and the airbag is fixed to the inner wall of the installation groove; a limiting ring is arranged at one end of the connecting pipe 15 away from the flexible hose 17, and an extension rod 19 is slidably connected to the inner wall of the limiting ring. One end of the extension rod 19 close to the air inlet cylinder 2 is fixed with a piston 18 slidably connected to the inner wall of the connecting pipe 15. A first spring 21 is connected between the piston 18 and the limiting ring. One end of the extension rod 19 away from the piston 18 is fixed with a baffle 20 located inside the air outlet cylinder 3. During the gas circulation process, when a certain air supply pipe 7 supplies air inward, the injected gas squeezes the airbag 16 at the corresponding position, so that the supplementary gas supplied is dispersed around through the airbag 16 deformed by the gas extrusion; and the airbag 16 is squeezed to make the piston 18 and the extension rod 19 move towards the air outlet cylinder 3, so that the baffle 20 at the corresponding position moves towards the connection air hole 4 of the air outlet cylinder 3, so that the staggered baffles 20 further disperse the flowing air when the gas is supplied, and further improve the uniform mixing efficiency of the circulating air flow.

[0044] Embodiment 3

[0045] On the basis of Embodiment 1 or Embodiment 2, refer to Figures 1 - 13 , a gas circulation system for a cell incubator, further comprising a plurality of dispersion components 28 arranged at the position between the outer wall of the dispersion cylinder 6 and the inner wall of the air supply cylinder 1. The dispersion component 28 is provided with a horizontally extending positioning rod 30. Two mounting blocks 29 are slidably connected to the outer wall of the positioning rod 30, and the mounting blocks 29 are fixedly connected to the inner wall of the air supply cylinder 1. Two third springs 35 are fixed at the position between the two mounting blocks 29 on the outer wall of the positioning rod 30. One end of each of the two third springs 35 away from each other abuts against the mounting block 29; a plurality of mounting holes are formed in the outer wall of the positioning rod 30, and a positioning block 31 is rotatably connected to the inner wall of the mounting hole through a torsion spring. One end of the positioning block 31 is fixed with an air flow blade 32, and the air flow blade 32 is inclined with respect to the axis of the positioning rod 30. Thus, during the process of gas circulation, the air flow impacts the inclined air flow blade 32 to drive the positioning rod 30 to rotate, so as to uniformly disperse the air flow in the circumferential direction.

[0046] In the present invention, refer to Figures 1 - 13, one end of the air supply pipe 7 extends to a position close to the inner wall of the air supply cylinder 1. One end of the air supply pipe 7 facing the inner wall of the air supply cylinder 1 is rotatably connected with an extension pipe 14 through a bearing. The end of the extension pipe 14 far from the air supply pipe 7 is close to the outer wall of the intake cylinder 2. A plurality of through holes 141 are provided at the position of the outer wall of the extension pipe 14 corresponding to the opening of the dispersion cylinder 6. An air groove 142 is provided on the outer wall of the extension pipe 14 at intervals with the through holes 141. The air groove 142 is inclined. During the process of gas circulation, part of the gas entering from the air supply pipe 7 is directly sent out from the bottom end of the extension pipe 14 along with the blowing wind force, and part of the gas flows out from the through holes 141 distributed at the bottom end of the periphery of the extension pipe 14 and collides with the air flow guided by the first air supply blade 12 to be dispersed; and as the air flow collides with the annularly distributed and inclined air groove 142, the extension pipe 14 rotates, so as to further improve the rapid and uniform dispersion when supplying supplementary gas.

[0047] In the present invention, referring to Figures 1 - 13 , an embedding groove is provided at the position of the outer wall of the extension pipe 14 corresponding to the positioning rod 30. A first magnet 143 is fixed on the inner wall of the embedding groove. A second magnetic attraction block 34 is fixed at the end of the positioning rod 30 close to the extension pipe 14. When supplying supplementary gas, the extension pipe 14 rotates along with the air flow movement, so that the first magnet 143 rotates and cooperates with the third spring 35 through magnetic attraction, so that the positioning rod 30 reciprocates horizontally along with the second magnetic attraction block 34, so that the positioning rod 30 and the air flow blade 32 rotate and move horizontally at the same time, so as to further improve the dispersion effect of the air flow.

[0048] In the present invention, referring to Figures 1 - 13 , a plurality of through holes are provided on the side wall of the fixed seat 5. A connecting rod 23 is slidably connected to the inner wall of the through hole. Sector-shaped moving parts 22 are fixed at both ends of the connecting rod 23. Elastic cloth 25 is connected between adjacent two moving parts 22, and between the moving parts 22 and the outer walls of the intake cylinder 2 and the outlet cylinder 3. Air flow grooves 26 are provided on the circumferential periphery of the outer walls on both sides of the moving parts 22. The moving part 22 close to the intake cylinder 2 is set as a first moving plate 221, and the moving part 22 close to the outlet cylinder 3 is set as a second moving plate 222. A second spring 24 is connected between the second moving plate 222 and the fixed seat 5. The outer diameter of the second moving plate 222 is larger than that of the first moving plate 221, so that the first moving plate 221 is adapted to the first air supply blade 12 and the second moving plate 222 is adapted to the second air supply blade 13. When a certain air supply pipe 7 injects gas inward, the horizontal impact force received by the corresponding first moving plate 221 is changed by guiding the air flow through the air flow grooves 26, so that the first moving plate 221 drives the second moving plate 222 to move horizontally under the action of air flow guidance, and while supplying supplementary gas, the horizontal movement dispersion effect of the air flow outside the intake cylinder 2 and the outlet cylinder 3 is improved.

[0049] In the present invention, referring to Figures 1 - 13, an auxiliary groove is provided at the edge position of the side wall of the movable member 22 close to the dispersion cylinder 6. A second magnet 27 is fixed to the inner wall of the auxiliary groove. A first magnetic attraction block 33 is fixed to the outer wall of the positioning rod 30 at a position corresponding to the movable member 22. The two first magnetic attraction blocks 33 are symmetrically distributed with respect to the positioning rod 30. Thus, when the movable member 22 moves horizontally, it will drive the positioning rod 30 to move horizontally through magnetic attraction with the first magnetic attraction block 33, so as to interact with the reciprocating movement of the extension pipe 14 driving the positioning rod 30, so that the air flow movement when the supplementary gas is supplied into the air supply pipe 7, the air flow in the two side areas of the fixed seat 5, and the air flow at the position of the dispersion assembly 28 cooperate and guide each other to disperse and flow; and when the first magnetic attraction block 33 rotates with the positioning rod 30 due to the symmetrical arrangement of the first magnetic attraction block 33, the magnetic attraction intensity between the first magnetic attraction block 33 and the second magnet 27 changes; thereby further improving the variability and uniformity of the dispersed supplementary air flow, avoiding the formation of air flow clusters due to always uniform movement, thereby further improving the uniformity of the dispersed air supply when the circulating air flow is supplied with gas and improving the working efficiency of uniform dispersion.

[0050] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered within the protection scope of the present invention.

Claims

1. A gas circulation system for a cell culture chamber, comprising a gas supply cylinder (1), one end of the gas supply cylinder (1) is fixed with a gas inlet cylinder (2), and the other end of the gas supply cylinder (1) is fixed with a gas outlet cylinder (3), characterized in that: The air inlet cylinder (2) and the air outlet cylinder (3) are blocked at one end inside the air supply cylinder (1), and a plurality of connecting air holes (4) are provided on the outer walls of the air inlet cylinder (2) and the air outlet cylinder (3) at positions inside the air supply cylinder (1); A fixed seat (5) of a disc-shaped structure is fixed between the air inlet cylinder (2) and the air outlet cylinder (3), and a dispersion cylinder (6) is rotatably connected to the circumferential outer wall of the fixed seat (5). Both ends of the dispersion cylinder (6) are open, and a gap is left between the outer wall of the dispersion cylinder (6) and the inner wall of the air supply cylinder (1); A plurality of air supply pipes (7) are fixedly penetrated through one end of the outer wall of the air supply cylinder (1) close to the air inlet cylinder (2).

2. A cell culture incubator gas circulation system according to claim 1, characterized in that: A connecting gear ring (8) is fixed to one end of the inner wall of the dispersion cylinder (6) close to the air inlet cylinder (2); a horizontally placed driving shaft (9) is rotatably connected to the inner wall of the air supply cylinder (1) at one end corresponding to the connecting gear ring (8) via a bearing; a driving gear (11) meshing with the connecting gear ring (8) is fixed to one end of the driving shaft (9); a driving motor (10) is transmission-connected to the other end of the driving shaft (9); and dispersion grooves (602) arranged at intervals and staggered are provided on the outer wall of the dispersion cylinder (6).

3. A cell culture incubator gas circulation system according to claim 2, characterized in that: A spirally distributed air supply blade 1 (12) is fixed at a position on the inner wall of the dispersion tube (6) corresponding to the air inlet tube (2), and a spirally distributed air supply blade 2 (13) is fixed at a position on the inner wall of the dispersion tube (6) corresponding to the air outlet tube (3), and both the air supply blade 1 (12) and the air supply blade 2 (13) supply air in the direction of the air inlet tube (2) as the dispersion tube (6) rotates; The outer diameters of the air inlet cylinder (2) and the air outlet cylinder (3) in the dispersion cylinder (6) gradually decrease toward the fixed seat (5), and the width of the air supply blade 1 (12) is greater than the width of the air supply blade 2 (13).

4. A cell culture incubator gas circulation system according to any one of claims 2 to 3, characterized in that: A plurality of connecting pipes (15) are fixedly and horizontally penetrated inside the air inlet cylinder (2) and the air outlet cylinder (3); one end of the connecting pipe (15) close to the air inlet cylinder (2) is connected to a hose (17); one end of the hose (17) is connected to an air bag; a mounting groove is provided at a position on the outer wall of the air inlet cylinder (2) corresponding to the air supply pipe (7); the air bag is fixed to the inner wall of the mounting groove; A limiting ring is provided at one end of the connecting tube (15) away from the hose (17); an extension rod (19) is slidably connected to the inner wall of the limiting ring; a piston (18) slidably connected to the inner wall of the connecting tube (15) is fixed at one end of the extension rod (19); a spring (21) is connected between the piston (18) and the limiting ring; and a baffle (20) located in the air outlet tube (3) is fixed at one end of the extension rod (19) away from the piston (18).

5. A cell culture incubator gas circulation system according to any one of claims 2 to 3, characterized in that: A plurality of dispersion components (28) are arranged between the outer wall of the dispersion cylinder (6) and the inner wall of the air supply cylinder (1), and the dispersion component (28) is provided with a horizontally extending positioning rod (30). The outer wall of the positioning rod (30) is slidably connected to two mounting blocks (29), and the mounting blocks (29) are fixedly connected to the inner wall of the air supply cylinder (1). Two springs (35) are fixedly arranged at a position on the outer wall of the positioning rod (30) between the two mounting blocks (29), and the ends of the two springs (35) that are far away from each other are both in contact with the mounting blocks (29); The outer wall of the positioning rod (30) is provided with a plurality of mounting holes, the inner wall of the mounting hole is rotatably connected to the positioning block (31) via a torsion spring, an air flow blade (32) is fixed to one end of the positioning block (31), and the air flow blade (32) is arranged obliquely with respect to the axis of the positioning rod (30).

6. A cell culture incubator gas circulation system according to claim 5, characterized in that: One end of the air supply pipe (7) facing the inner wall of the air supply cylinder (1) is rotatably connected to an extension pipe (14) via a bearing; a plurality of through holes (141) are provided on the outer wall of the extension pipe (14) at positions corresponding to the opening of the dispersion cylinder (6); and air grooves (142) are provided on the outer wall of the extension pipe (14) at intervals from the through holes (141); the air grooves (142) are arranged obliquely.

7. A cell culture incubator gas circulation system according to claim 6, characterized in that: An embedding groove is provided at a position on the outer wall of the extension tube (14) corresponding to the positioning rod (30), a magnetic block 1 (143) is fixed to the inner wall of the embedding groove, and a magnetic block 2 (34) is fixed to one end of the positioning rod (30) close to the extension tube (14).

8. The cell culture incubator gas circulation system according to claim 5, characterized in that: The side wall of the fixing seat (5) is provided with a plurality of through holes, the inner wall of the through hole is slidably connected with a connecting rod (23), both ends of the connecting rod (23) are fixed with movable parts (22) of fan-shaped structure, elastic cloth (25) is connected between two adjacent movable parts (22), and between the movable parts (22) and the outer walls of the air inlet cylinder (2) and the air outlet cylinder (3), and air flow grooves (26) are provided on the circumference of the outer walls on both sides of the movable parts (22).

9. A cell culture incubator gas circulation system according to claim 8, characterized in that: The movable part (22) near the air inlet (2) is configured as a movable plate 1 (221), and the movable part (22) near the air outlet (3) is configured as a movable plate 2 (222). A spring 2 (24) is connected between the movable plate 2 (222) and the fixed seat (5), and the outer diameter of the movable plate 2 (222) is greater than the outer diameter of the movable plate 1 (221).

10. A cell culture incubator gas circulation system according to claim 9, characterized in that: An auxiliary groove is provided on the side wall of the movable part (22) near the edge of the dispersion tube (6), a second magnetic block (27) is fixed on the inner wall of the auxiliary groove, and a first magnetic block (33) is fixed on the outer wall of the positioning rod (30) at a position corresponding to the movable part (22), and the two first magnetic blocks (33) are symmetrically distributed with respect to the positioning rod (30).

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