Vibration device and separation method

Abstract

A vibration device and a separation method belong to the technical field of cell culture, the vibration device comprises a workbench, the workbench is provided with a mounting groove for placing a culture vessel, the vibration device is characterized in that the workbench is provided with a vibration mechanism and a limiting mechanism, and the vibration mechanism and the limiting mechanism are oppositely arranged on the upper side and the lower side of the mounting groove; the vibrating mechanism comprises a vertically arranged motor, an output shaft of the motor is fixedly connected with a first rotating cylinder, a first guide groove is formed in the outer wall of the first rotating cylinder in the circumferential direction of the first rotating cylinder, the first guide groove is in a continuous broken line shape, the first guide groove is slidably connected with a connecting shaft, and the connecting shaft is fixedly connected with an impact assembly. The rotary motion of the motor can be converted into reciprocating vertical motion of the connecting shaft and the impact assembly, periodic rigid impact is generated, the diversified requirements of different cell culture experiments for vibration strength and frequency are met, and a high-cost high-rotating-speed motor does not need to be used.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, and in particular to a vibration device and a separation method. Background Art

[0002] Adherent cells are a type of cells that need to attach to a solid surface to grow and reproduce when cultured in vitro. Adherent cells secrete extracellular matrix, interact with the surface of the culture vessel, and achieve cell spreading and migration through the regulation of the cytoskeleton. During the culture process, when the cell proliferation reaches a certain density, contact inhibition occurs, that is, the cells stop proliferating and migrating after contacting each other to maintain the cell monolayer state.

[0003] When the adherent cells proliferate to the point where they cover the entire surface of the culture vessel, they need to be separated from the culture vessel and collected and processed after the cells are dispersed. There are many ways to separate and disperse adherent cells, such as centrifugal dispersion, vortex dispersion or physical vibration. Among them, the physical vibration method will not produce strong convection and shear force in the culture vessel, and will cause less damage to the cells. Therefore, physical vibration is currently used to separate and disperse adherent cells from the culture vessel.

[0004] In order to avoid convection and shear force, the existing physical vibration method is to place the culture vessel on a vibration device, and use a motor to drive a reciprocating vibration rod or other vibration structure to impact the bottom of the culture vessel. Due to the limitation of the motor speed, the frequency of the reciprocating movement of the motor-driven vibration rod is limited. When high-frequency vibration is required, it is necessary to replace it with a high-speed motor, which is costly. Summary of the invention

[0005] In order to solve the technical problem that the existing vibration device in the above-mentioned background technology is limited by the motor speed, resulting in the frequency of the reciprocating movement of the vibration rod being limited, and when high-frequency vibration is required, a high-speed motor needs to be used, which has high cost of use, the present invention provides a vibration device.

[0006] The technical solution of the present invention is as follows: The present invention provides a vibration device, including a workbench, a mounting groove for placing a culture vessel is provided on the workbench, a vibration mechanism and a limit mechanism are installed on the workbench, and the vibration mechanism and the limit mechanism are relatively arranged on the upper and lower sides of the mounting groove; the vibration mechanism includes a vertically arranged motor, the output shaft of the motor is fixedly connected to a first rotating drum, a first guide groove is opened on the outer wall of the first rotating drum along its circumferential direction, the first guide groove is a continuous broken line shape, the first guide groove is slidably connected to a connecting shaft, the connecting shaft is fixedly connected to an impact assembly, the connecting shaft is fixedly connected to the impact assembly, and the rotational motion of the motor can be converted into reciprocating vertical motion of the connecting shaft and the impact assembly, generating periodic rigid impact, and providing a stable power source for the culture vessel, the broken line-shaped first guide groove can accurately control the motion trajectory and impact strength of the impact assembly, and meet the diverse requirements of different cell culture experiments for vibration intensity and frequency, without using a high-cost high-speed motor.

[0007] Preferably, the impact assembly includes a fixed block fixedly arranged on the workbench, the fixed block is provided with a vertically arranged guide groove, a guide rod is slidably connected in the guide groove, the outer wall of the guide rod is fixedly connected to the connecting shaft, and a plurality of vibration protrusions are fixedly provided on the top of the guide rod. The arrangement of the fixed block and the guide groove ensures the stability and accuracy of the guide rod movement, so that it can reciprocate in a specified vertical direction. The vibration protrusion increases the contact area between the impact assembly and the culture vessel and the unevenness of the impact force, thereby improving the efficiency and effect of vibration transmission, which is conducive to promoting the detachment of adherent cells.

[0008] Preferably, two impact assemblies are provided, the two impact assemblies are relatively arranged on both sides of the first rotating drum, the two impact assemblies are respectively connected to the first guide groove through a connecting shaft, the two connecting shafts are arranged up and down, and the two relatively arranged impact assemblies can generate a more balanced impact force, avoiding uneven force on the device that may be caused by a single impact assembly. The connecting shafts arranged up and down cooperate with the first guide groove so that the movements of the two impact assemblies have a certain phase difference, resulting in an alternating impact effect, further enhancing the impact frequency and effect on the culture vessel, and improving the efficiency of cell dispersion.

[0009] Preferably, the output shaft of the motor is fixedly connected to the first rotating drum and the second rotating drum in sequence along the axial direction, and a second guide groove is provided on the outer wall of the second rotating drum along its circumferential direction, and the second guide groove is a continuous broken line shape, and the first guide groove and the second guide groove are respectively connected to an impact assembly through a connecting shaft, and the two impact assemblies are distributed on both sides of the motor output shaft, and the highest point of the first guide groove is close to the lowest point of the second guide groove. The impact assembly connected by the two rotating drums and different guide grooves produces an alternating impact effect, which can further optimize the impact effect on the culture vessel, adapt to the separation requirements of different types of adherent cells, and improve the success rate and quality of cell separation.

[0010] Preferably, the top of the vibration protrusion is hemispherical, which can reduce the damage of the vibration protrusion to the culture vessel.

[0011] Preferably, the top of the vibration protrusion is a buffer head, which plays a buffering role and reduces damage to the culture vessel.

[0012] Preferably, the limiting mechanism includes a first limiting plate and a second limiting plate which are relatively arranged. The bottom of the first limiting plate and the second limiting plate are respectively fixedly connected to a pressure plate by a spring. The pressure plate can be used to press and limit the culture vessel to ensure that the culture vessel remains stable during the vibration process, avoiding the vibration effect and cell separation effect affected by the movement of the culture vessel, and further ensuring the use effect of the vibration device.

[0013] Preferably, a bubble filling mechanism is provided on the workbench, the bubble filling mechanism includes a nano bubble generating unit, the nano bubble generating unit is respectively connected to an air source, a water source and a filling head, the bubble filling mechanism can add a mixed solution containing nano bubbles into the culture vessel, the nano bubbles and the vibration work synergistically to generate microjets to impact cells, synergistically promote cell detachment, greatly improve the efficiency and quality of cell dispersion, and provide better cell samples for cell culture and subsequent experiments.

[0014] A separation method, the specific process is: Place the culture vessel in the installation slot and press it with the limit mechanism, start the bubble filling mechanism, and use the filling head to fill the mixed liquid containing nanobubbles into the culture vessel. The nanobubbles and vibration work together to produce microjets to impact cells and promote cell detachment, which greatly improves the efficiency and quality of cell dispersion and provides better cell samples for cell culture and subsequent experiments. The vibration mechanism is started and impacts the bottom of the culture vessel. During the vibration process, the vibration induces the nanobubbles to burst, generating microjets to impact the cells. The bubbles carry digestive enzymes and release them in a directional manner, which synergistically promotes cell detachment. Through the synergistic effect of the vibration mechanism and nanobubbles, the adherent cells are effectively caused to detach from the culture vessel and disperse. Specifically, the vibration mechanism provides vibration, and the nanobubbles burst during the vibration process to generate microjets and directional release of digestive enzymes, which greatly improves the efficiency of cell dispersion, while reducing damage to cells, ensuring cell activity, and providing high-quality cell samples for subsequent cell experiments.

[0015] Preferably, the vibration mechanism uses two impact components, and the two guide rods of the two double-click components make opposite movements. The two guide rods are used to perform alternating reciprocating impacts on the bottom of the culture vessel, thereby enhancing the uniformity of the impact frequency and force on the culture vessel, thereby improving the vibration transmission efficiency and cell dispersion effect of the entire device, ensuring that the adherent cells can be detached and dispersed from the culture vessel more comprehensively and quickly.

[0016] It can be seen from the above technical solutions that the advantages of the present invention are: 1. A continuous zigzag first guide groove is provided on the outer wall of the first rotating drum, which can convert the rotational motion of the motor into the reciprocating vertical motion of the connecting shaft and the impact assembly, generate periodic rigid impact, and provide a stable power source for the culture dish. The zigzag first guide groove can accurately control the motion trajectory and impact force of the impact assembly, meet the diverse requirements of different cell culture experiments on vibration intensity and frequency, and do not need to use high-cost high-speed motors.

[0017] 2. Two impact components set relatively to each other can produce a more balanced impact force, avoiding the uneven force on the device that may be caused by a single impact component. The movement of the two impact components has a certain phase difference, producing an alternating impact effect. Without increasing the motor speed, the impact frequency and effect on the culture vessel are further enhanced, thereby improving the efficiency of cell dispersion. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solution of the present invention, the accompanying drawings required for use in the description will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0019] Figure 1 is a schematic diagram of the overall structure of a vibration device according to one or more embodiments of the present invention; Figure 2 is a schematic structural diagram of a vibration mechanism according to one or more embodiments of the present invention; Figure 3 It is a schematic structural diagram of a limiting mechanism according to one or more embodiments of the present invention; Figure 4 A schematic diagram of the principle of a bubble filling mechanism according to one or more embodiments of the present invention; The components represented by the reference numerals in the figure are: 1. Workbench; 2. Vibration mechanism; 3. Limiting mechanism; 4. Bubble filling mechanism; 5. Motor; 6. First rotating drum; 7. Second rotating drum; 8. First guide groove; 9. Second guide groove; 10. Connecting shaft; 11. Impact assembly; 12. Guide rod; 13. Fixed block; 14. Guide groove; 15. Connecting plate; 16. Vibration protrusion; 17. First limiting plate; 18. Second limiting plate; 19. Pressing plate; 20. Spring; 21. First pillar; 22. Second pillar; 23. Slide plate; 24. Mounting groove; 25. Nano bubble generating unit; 26. Air source; 27. Water source; 28. Filling head; 29. ​​Slide groove. DETAILED DESCRIPTION

[0020] In order to make the purpose, features and advantages of the present invention more obvious and easy to understand, the technical scheme of the present invention will be clearly and completely described below in conjunction with the drawings in this specific embodiment. Obviously, the embodiments described below are only part of the embodiments of the present invention, not all of them. Based on the embodiments in this patent, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this patent.

[0021] Example 1 In a typical embodiment of the present invention, Figure 1-Figure 4 As shown, a vibration device is proposed, comprising: a workbench 1, a vibration mechanism 2, a limiting mechanism 3 and a bubble filling mechanism 4, wherein the vibration mechanism 2, the limiting mechanism 3 and the bubble filling mechanism 4 are all installed on the workbench 1, the limiting mechanism 3 is installed above the vibration mechanism 2, and the limiting mechanism 3 is used to limit the position of the culture vessel; the bubble filling mechanism 4 is used to artificially inject nanobubbles into the culture vessel, and during operation, the vibration induces the nanobubbles to rupture, generating microjets to impact cells, so as to reduce the required mechanical amplitude and improve the detachment and dispersion efficiency of the cells, and digestive enzymes can also be added to the bubbles, so that the bubbles carry digestive enzymes (such as pancreatin) and are released in a directional manner, so as to further synergistically promote cell detachment.

[0022] Specifically, a mounting groove 24 is provided on the workbench 1, and the mounting groove 24 runs through the table top of the workbench 1 for placing a culture vessel. The mounting groove 24 is a circular groove, and the diameter of the mounting groove 24 is smaller than the diameter of the ear plate of the culture vessel, so that the culture vessel can be placed in the mounting groove 24, and the culture vessel can use its own ear plate to support and position the culture vessel; the vibration mechanism 2 is located below the table top of the workbench 1 and directly below the mounting groove 24, so as to impact the bottom of the culture vessel; the limiting mechanism 3 is installed on the table top of the workbench 1, so as to press the ear plate of the culture vessel to further limit the culture vessel.

[0023] like Figure 2As shown, the vibration mechanism 2 includes a motor 5, a first rotating drum 6, a connecting shaft 10 and an impact assembly 11. The motor 5 is vertically arranged, and the impact assembly 11 is connected to the first rotating drum 6 through the connecting shaft 10. Specifically, the impact assembly 11 includes a guide rod 12, a guide groove 14 and a vibration protrusion 16. The first rotating drum 6 is fixedly provided with a rotating shaft, and the first rotating drum 6 is fixedly connected to the output shaft of the motor 5 through the rotating shaft, so that the first rotating drum 6 is driven by the motor 5 to rotate around the axis. A first guide groove 8 is opened on the outer wall of the first rotating drum 6 along its annular direction. The first guide groove 8 is a continuous broken line shape, and the first guide groove 8 contains the highest point and the lowest point. The guide rod 12 is slidably arranged in the guide groove 14, and the guide groove 14 is vertically opened in the fixed block 13. The upper and lower ends of the guide groove 14 The fixing block 13 is fixedly installed on the workbench 1 by welding or bolt connection, a connecting shaft 10 is fixedly connected to the outer wall of the guide rod 12, the connecting shaft 10 is slidably arranged in the first guide groove 8, the guide rod 12 is vertically arranged and the axial direction of the guide rod 12 is parallel to the axial direction of the first rotating drum 6, the top of the guide rod 12 contacts the bottom of the culture vessel through the vibration protrusion 16, when the motor 5 drives the first rotating drum 6 to rotate around the axis, the connecting shaft 10 can move along the trajectory of the first guide groove 8, so that the position of the connecting shaft 10 is switched back and forth between the highest point and the lowest point of the first guide groove 8, the connecting shaft 10 is used to drive the guide rod 12 to move vertically back and forth, and the vibration protrusion 16 can reciprocate and impact the bottom of the culture vessel to generate vibration.

[0024] It is understandable that, in actual use, the amplitude and period of the first guide groove 8 can be changed to meet different vibration frequency and intensity requirements, which can be confirmed according to actual design requirements, and no excessive restrictions are made here.

[0025] In this embodiment, the cross-sections of the guide rod 12 and the guide groove 14 are both rectangular. In other embodiments, the cross-sections of the guide rod 12 and the guide groove 14 may also be circular, triangular or other shapes. There are no excessive restrictions on the specific shapes, as long as the cross-sections of the guide rod 12 and the guide groove 14 are the same.

[0026] In order to further increase the vibration frequency, a second rotating drum 7 is fixedly provided on the output shaft of the motor 5. The first rotating drum 6 and the second rotating drum 7 are coaxially arranged and arranged in sequence along the axial direction of the output shaft. The first rotating drum 6 and the second rotating drum 7 are fixedly connected to the output shaft of the motor 5 through the same rotating shaft, so that the first rotating drum 6 and the second rotating drum 7 can be driven to rotate synchronously around the axis under the drive of the motor 5. A second guide groove 9 is opened on the outer wall of the second rotating drum 7 along its circumferential direction. The second guide groove 9 is also a continuous broken line shape. The second rotating drum 7 is slidably connected to a A connecting shaft 10 is provided, and the connecting shaft 10 is fixedly connected with an impact assembly 11. The impact assembly 11 connected to the second rotating drum 7 also includes a guide rod 12. The guide rod 12 is slidably connected to a guide groove 14, and a vibration protrusion 16 is fixedly provided on the top of the guide rod 12 to reciprocately impact the bottom of the culture vessel through the vibration protrusion 16 to generate vibration. In this embodiment, the two impact assemblies 11 are relatively arranged on both sides of the axial direction of the first rotating drum 6 and the second rotating drum 7 (which can also be understood as both sides of the output shaft of the motor 5) to alternately reciprocate and impact the culture vessel.

[0027] The first guide groove 8 and the second guide groove 9 have opposite folding directions. Figure 2 As shown, the highest point of the first guide groove 8 is close to the lowest point of the second guide groove 9, and the lowest point of the first guide groove 8 is close to the highest point of the second guide groove 9, so that during the synchronous rotation of the first rotating drum 6 and the second rotating drum 7 around the axis, the two guide rods 12 can make opposite movements, that is, when one guide rod 12 moves upward, the other guide rod 12 moves downward, so that when a low-speed motor 5 is used instead of replacing the high-speed motor 5, the vibration frequency of the guide rod 12 on the culture vessel can be effectively improved, and the use cost is effectively reduced.

[0028] In order to simplify the structure, in other embodiments, the second drum 7 may be eliminated so that the first drum 6 is connected to two impact assemblies 11 at the same time. In this case, the two impact assemblies 11 are relatively distributed on both sides of the first drum 6 .

[0029] Specifically, the two impact assemblies 11 are relatively distributed on both sides of the first rotating drum 6, and the guide rods 12 of the two impact assemblies 11 are vertically arranged. The two guide rods 12 are slidably connected to the first guide groove 8 on the first rotating drum 6 through a connecting shaft 10 respectively, and the two connecting shafts 10 are arranged up and down. In this embodiment, in the non-working state, one connecting shaft 10 is located at the highest point of the first guide groove 8, and the other connecting shaft 10 is located at the lowest point of the first guide groove 8. Therefore, during the rotation of the first rotating drum 6 around the axis, the two guide rods 12 can make opposite movements, that is, when one guide rod 12 moves upward, the other guide rod 12 moves downward. Therefore, under the condition of using one motor 5 and not increasing the speed of the motor 5, the vibration frequency of the guide rod 12 on the culture vessel can be effectively improved, and there is no need to set up two rotating drums, which simplifies the overall structure of the vibration mechanism 2 and is more convenient for later maintenance work.

[0030] In this embodiment, a motor 5 is used to directly drive the rotating drum to rotate around the axis, and the guide groove on the rotating drum is used to drive the connecting shaft 10 connected thereto to move along the trajectory of the guide groove, thereby driving the guide rod 12 to move vertically back and forth to achieve impact on the bottom of the culture vessel. Compared with the existing eccentric shaft and connecting rod structure, the vertical reciprocating movement of the guide rod 12 is directly driven by the guide groove, which reduces the use of complex structures, simplifies the overall structure, reduces the production and use costs, and does not use intermediate transmission structures such as eccentric shafts and connecting rods. Through the setting of the guide groove, the moving trajectories of the connecting shaft 10 and the guide rod 12 can be accurately controlled to achieve high-precision reciprocating motion, and the equipment has high operating efficiency.

[0031] There are a plurality of vibration protrusions 16, which are fixedly arranged at intervals on the upper surface of the connecting plate 15 by welding or integral molding, and the connecting plate 15 is horizontally fixedly installed on the top of the guide rod 12 by welding or threaded connection. The vibration protrusions 16 increase the contact area with the culture vessel, so that more energy can be transmitted during the impact process, thereby enhancing the vibration effect. In addition, the arrangement of a plurality of vibration protrusions 16 can perform multiple impacts on the culture vessel when the guide rod 12 moves back and forth vertically, thereby increasing the frequency and intensity of the vibration.

[0032] In actual use, in order to facilitate maintenance, the connecting plate 15 can be connected to the guide rod 12 by a threaded connection. Specifically, the top of the guide rod 12 is provided with an external thread, and the bottom of the connecting plate 15 is provided with a threaded hole, so that the connecting plate 15 and the guide rod 12 can be quickly disassembled and assembled by the threaded connection.

[0033] It should be noted that the top of the vibration protrusion 16 cannot be a pointed structure. Its top can be set to a hemispherical shape, a round cake shape, etc. to reduce the damage of the vibration protrusion 16 to the culture vessel. In addition, a rubber buffer head can be set on the top of the vibration protrusion 16 to reduce damage to the bottom of the culture vessel.

[0034] like Figure 3 As shown, the limiting mechanism 3 includes a first limiting plate 17, a second limiting plate 18, a pressure plate 19, a spring 20, a first pillar 21, a second pillar 22 and a slide plate 23, wherein the first limiting plate 17, the second limiting plate 18 and the pressure plate 19 are all arc-shaped plate structures, the bottom of the first limiting plate 17 is fixedly connected to a pressure plate 19 through a plurality of springs 20, and the first limiting plate 17 is fixedly installed on the workbench 1 through the first pillar 21; the second limiting plate 18 is arranged opposite to the first limiting plate 17, and the bottom of the second limiting plate 18 is fixedly connected to a pressure plate 19 through a plurality of springs 20. The second limiting plate 18 is fixedly connected, and the bottom of the second limiting plate 18 is fixedly connected to the second pillar 22, and the bottom of the second pillar 22 is fixedly connected to the slide plate 23; a slide groove 29 is opened on the table top of the workbench 1, and the slide plate 23 is slidably set in the slide groove 29, and the slide plate 23 is provided with a mounting hole. A plurality of bolt holes are arranged at intervals along the length direction of the slide groove 29. The second limiting plate 18 can adjust the relative position with the first limiting plate 17, and the position of the second limiting plate 18 can be fixed by bolts; the pressing plate 19 is used to press and limit the ear plate of the culture vessel, and at the same time plays a buffering role.

[0035] The bubble filling mechanism 4 includes a nano bubble generating unit 25, a gas source 26, a water source 27 and a filling head 28, wherein the nano bubble generating unit 25 is an existing nano bubble generator, which can mix gas (such as air, nitrogen, oxygen, etc.) with water to form tiny bubbles with a diameter between tens of nanometers and several micrometers; the gas source 26 includes a gas cylinder, a compressor and a gas regulating valve, the compressor is connected to the gas cylinder and the air inlet of the nano bubble generating unit 25 through a pipeline, and the gas regulating valve is arranged on the pipeline to transport gas to the nano bubble generating unit 25; the water source 27 includes a water tank, a water pump and a valve, the water pump is arranged in the water tank and connected to the water inlet of the nano bubble generating unit 25 through a circulation pipeline, and the valve is arranged on the circulation pipeline for water delivery, so that the nano bubble generating unit 25 outputs a mixed liquid containing nano bubbles; the filling head 28 is connected to the output port of the nano bubble generating unit 25 through a pipeline, and the filling head 28 is in the shape of a slender nozzle, so as to form a fine liquid flow, and can receive the mixed liquid containing nano bubbles and inject it into the culture vessel.

[0036] It is understandable that digestive enzymes may be added to the water of the water source 27 as required to facilitate the subsequent detachment and dispersion of the auxiliary cells.

[0037] Example 2 In another typical embodiment of the present invention, a separation method is proposed, using the vibration device mentioned in Example 1, and the specific process is as follows: A culture vessel for culturing adherent cells is placed in the mounting groove 24, and the ear plates of the culture vessel are pressed and limited by two pressing plates 19, the nanobubble generating unit 25 is started, the gas source 26 and the water source 27 work simultaneously, the gas source 26 delivers the required gas to the nanobubble generating unit 25 through a pipeline, and the water source 27 delivers the required water to the nanobubble generating unit 25 through a circulation pipeline, the nanobubble generating unit 25 mixes the gas with the water to form nanobubbles, and then the culture vessel is opened, and the mixed liquid containing nanobubbles is added to the culture vessel by using the filling head 28, and the culture vessel is closed after the filling is completed; After the nanobubble filling is completed, the nanobubble generating unit 25 is stopped and the vibration mechanism 2 is started. The vibration mechanism 2 impacts the bottom of the culture vessel from below, causing the adherent cells to detach from the culture vessel and disperse. During the vibration process, the vibration induces the nanobubbles to rupture, generating microjets to impact the cells, and the bubbles carry the digestive enzymes for directional release, which synergistically promotes the cell detachment while reducing the required mechanical amplitude.

[0038] Specifically, when the motor 5 is connected to a first rotating drum 6, and the first rotating drum 6 is connected to an impact assembly 11, the motor 5 drives the first rotating drum 6 to rotate around the axis, and the connecting shaft 10 can move along the trajectory of the first guide groove 8, so that the position of the connecting shaft 10 switches back and forth between the highest point and the lowest point of the first guide groove 8, and the connecting shaft 10 drives the corresponding guide rod 12 to move back and forth vertically, and the vibration protrusion 16 is used to reciprocate and impact the bottom of the culture vessel; When the motor 5 is connected to a first rotating drum 6, an impact assembly 11 is respectively connected to both sides of the first rotating drum 6, and the two connecting shafts 10 are arranged up and down, the motor 5 drives the first rotating drum 6 to rotate around the axis, and the two connecting shafts 10 arranged up and down respectively move along the trajectory of the first guide groove 8, so that the positions of the two connecting shafts 10 are alternately switched back and forth between the highest point and the lowest point of the first guide groove 8, that is, one connecting shaft 10 is located at the highest point of the first guide groove 8, and the other connecting shaft 10 is located at the lowest point of the first guide groove 8, so as to drive the two guide rods 12 to do opposite movements, that is, when one guide rod 12 moves upward, the other guide rod 12 moves downward, and the two guide rods 12 are used to perform alternating reciprocating impacts on the bottom of the culture vessel, thereby improving the vibration efficiency; When the motor 5 is connected to a first rotating drum 6 and a second rotating drum 7 at the same time, the first rotating drum 6 and the second rotating drum 7 are arranged up and down, the first rotating drum 6 is connected to an impact assembly 11, the second rotating drum 7 is connected to an impact assembly 11, and the two impact assemblies 11 are arranged oppositely; the motor 5 drives the first rotating drum 6 and the second rotating drum 7 to rotate synchronously around the axis, and the connecting shafts 10 of the two impact assemblies 11 move along the corresponding first guide groove 8 and the second guide groove 9 respectively to drive the two guide rods 12 to make opposite movements, that is, when one guide rod 12 moves upward, the other guide rod 12 moves downward, and the two guide rods 12 are used to perform alternating reciprocating impacts on the bottom of the culture vessel to improve the vibration efficiency. The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A vibration device, comprising: A workbench (1), wherein a mounting groove (24) for placing a culture vessel is provided on the workbench (1), characterized in that a vibration mechanism (2) and a limiting mechanism (3) are installed on the workbench (1), and the vibration mechanism (2) and the limiting mechanism (3) are arranged relatively on the upper and lower sides of the mounting groove (24); The vibration mechanism (2) comprises a vertically arranged motor (5), the output shaft of the motor (5) being fixedly connected to a first rotating drum (6), a first guide groove (8) being provided on an outer wall of the first rotating drum (6) along its circumferential direction, the first guide groove (8) being in a continuous broken line shape, a connecting shaft (10) being slidably connected to the first guide groove (8), and the connecting shaft (10) being fixedly connected to an impact assembly (11).

2. The vibration device according to claim 1, characterized in that The impact assembly (11) comprises a fixed block (13) fixedly mounted on the workbench (1), the fixed block (13) being provided with a vertically arranged guide groove (14), the guide groove (14) being slidably connected to a guide rod (12), the outer wall of the guide rod (12) being fixedly connected to the connecting shaft (10), and a plurality of vibration protrusions (16) being fixedly mounted on the top of the guide rod (12).

3. The vibration device according to claim 1, characterized in that Two impact assemblies (11) are provided. The two impact assemblies (11) are arranged oppositely on two sides of the first rotating drum (6). The two impact assemblies (11) are connected to the first guide groove (8) via a connecting shaft (10) respectively. The two connecting shafts (10) are arranged up and down.

4. The vibration device according to claim 1, characterized in that The output shaft of the motor (5) is fixedly connected to the first rotating drum (6) and the second rotating drum (7) in sequence along the axial direction. A second guide groove (9) is provided on the outer wall of the second rotating drum (7) along its circumferential direction. The second guide groove (9) is in a continuous broken line shape. The first guide groove (8) and the second guide groove (9) are respectively connected to an impact assembly (11) via a connecting shaft (10). The two impact assemblies (11) are distributed on both sides of the output shaft of the motor (5). The highest point of the first guide groove (8) is close to the lowest point of the second guide groove (9).

5. The vibration device according to claim 2, characterized in that The top of the vibration protrusion (16) is hemispherical.

6. The vibration device according to claim 2, characterized in that The top of the vibration protrusion (16) is a buffer head.

7. The vibration device according to claim 1, characterized in that The limiting mechanism (3) comprises a first limiting plate (17) and a second limiting plate (18) which are arranged opposite to each other, and the bottoms of the first limiting plate (17) and the second limiting plate (18) are respectively fixedly connected to a pressing plate (19) via a spring (20).

8. The vibration device according to claim 1, characterized in that A bubble filling mechanism (4) is provided on the workbench (1), the bubble filling mechanism (4) comprising a nano bubble generating unit (25), and the nano bubble generating unit (25) is respectively connected to an air source (26), a water source (27) and a filling head (28).

9. A separation method, characterized in that: The vibration device according to any one of claims 1 to 8 is used, and the specific process is as follows: The culture vessel is placed in the installation groove (24) and pressed tightly by the limiting mechanism (3), the bubble filling mechanism (4) is started, and the mixed liquid containing nanobubbles is filled into the culture vessel by the filling head (28); The vibration mechanism (2) is started to impact the bottom of the culture vessel. During the vibration process, the vibration induces the nanobubbles to burst, generating microjets to impact the cells. The bubbles carry digestive enzymes for directional release, thereby synergistically promoting cell detachment.

10. The separation method according to claim 9, characterized in that The vibration mechanism (2) uses two impact components (11), and two guide rods (12) of the two double-acting components move in opposite directions, so that the two guide rods (12) are used to perform alternating reciprocating impacts on the culture vessel.

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