Filtering device and filtering method for biological sample detection

Abstract

The invention relates to the technical field of solid-liquid separation, in particular to a filtering device for biological sample detection and a filtering method.The filtering device comprises a machine base, a sample carrying table, a filtering unit, a liquid transferring unit and a control unit.The sample carrying table is provided with a filtering pipe containing area, a to-be-treated sample area, a filtered sample area, a clean suction head storage area and a waste area at intervals; a reverse filter pipe is arranged in the filter pipe placing area, and an outer sleeve is arranged in the to-be-treated sample area. Through the cooperation of the reverse filter tube, the outer sleeve, the filter unit and the pipetting unit, the pretreatment process of a biological sample is realized, meanwhile, the whole treatment process is convenient and rapid, the time is saved, and the purpose of high-flux solid-liquid separation is also realized.

Description

Technical Field

[0001] This application relates to the technical field of solid-liquid separation, and in particular to a filtering device and a filtering method for biological sample detection. Background Art

[0002] The detection of biological samples is an important category in the field of detection. Biological samples mainly include animal samples, plant samples, grain samples, feed samples, body fluid samples, and some soil samples, etc. In the process of biological sample detection, the pre-treatment process of the sample is a very important link. More than 70% of the errors in the detection results come from the sampling and sample processing process. Therefore, efficient and unbiased pre-treatment of samples is of great significance for ensuring the accuracy of detection results.

[0003] In the pre-treatment process of samples, the steps usually included are: sample crushing, extraction of substances to be detected in the sample, solid-liquid separation, purification and enrichment of substances to be detected, elution and purification of substances to be detected, etc. Among them, the centrifugation operation is usually adopted in the solid-liquid separation link, that is, in a centrifuge, centrifugation is carried out at a certain rotational speed for 10 - 30 minutes to achieve solid-liquid separation. For subsequent operations, either the precipitate generated after centrifugation is taken, or the supernatant is taken for the next operation. In the detection process, the usual centrifugation is to centrifuge to remove impurities or other substances that affect detection, such as soil particles, large grain fragments of grain, cell fragments, large particle plant residues after crushing, etc. For example, in the national standard GB 5009.22 for the detection of aflatoxin in grains, it is clearly required to centrifuge at 6000 rpm for 10 min, and then take the supernatant for the next operation. There are many similar requirements, such as GB5009.111, GB5009.118, GB5009.240, etc.

[0004] The existing centrifugation operation method has several disadvantages. First, it takes a long time. Centrifugation usually takes 10 minutes and even longer. Adding the acceleration and deceleration time of the centrifuge during the centrifugation process, the time consumption will be even longer. Especially when multiple steps in the operation process require centrifugation, the operation time will be very long, seriously affecting the efficiency of sample detection. Second, for biological samples, the precipitate obtained after centrifugation is not firm. In the subsequent operations of taking and placing the centrifuge tube and sucking the supernatant, the precipitate is easily shaken, resulting in the centrifugation product being in a turbid or partially turbid state again, seriously affecting the subsequent operations. Third, due to the strong centrifugal force generated during centrifugation, a large counterweight module needs to be set in the centrifuge, which limits the automation of the centrifugation action in the sample pre-treatment operation. Finally, the rotor volume of the centrifuge also limits the number of samples that can be processed, thus affecting the throughput of sample pre-treatment.

[0005] The problems encountered in centrifuging some samples can be solved by using filtration technology. Common filtration devices mainly perform forward filtration, that is, after crushing the sample, an extraction solution is added, and then the mixture is loaded into a filtration tube or filtration column with a filter membrane for filtration. This filtration method requires a filtration pressure to make the filtrate pass through the filtration medium under pressure; if it is an ultrafiltration tube, it is usually used in combination with a centrifuge. The substance to be separated is loaded into the ultrafiltration tube, and the ultrafiltration tube is loaded into a sleeve, and separation is achieved under the action of the centrifuge; if filtration methods such as ultrafiltration membrane packages and hollow fiber membranes are used, first, this kind of filtration method also requires a peristaltic pump or corresponding equipment to provide the filtration pressure. Second, since the particle size distribution of the crushed biological sample is non-uniform, multiple filtrations with filter membranes of different pore sizes are required to achieve the filtration effect, which not only increases the time but also increases the consumables; if the filtration column method is used, a filter element or filter membrane is loaded inside the column lumen, then pressure needs to be applied at the top of the column or the column needs to be connected to a powered filtration system to achieve this. This kind of filtration operation also requires a filtration device that provides pressure. In the pretreatment process of biological sample detection, solid-liquid separation is a relatively rough step, only the particulate impurities need to be removed, and it is not necessary to finely separate the impurities that are invisible to the naked eye in the liquid to be detected.

[0006] Based on the above background, in the sample pretreatment process of biological sample detection, there is an urgent need for a convenient, fast, time-saving and high-throughput solid-liquid separation method. Summary of the Invention

[0007] In order to achieve the purpose of ensuring a convenient, fast, time-saving and high-throughput solid-liquid separation in the sample pretreatment process of biological sample detection, the present application provides a filtration device and a filtration method for biological sample detection.

[0008] In the first aspect, a filtration device for biological sample detection provided by the present application adopts the following technical solutions: A filtration device for biological sample detection includes a machine base, a sample loading platform arranged on the machine base, a filtration unit, a pipetting unit and a control unit. The sample loading platform is provided with a filtration tube placement area, a to-be-treated sample area, a filtered sample area, a clean pipette tip storage area and a waste area at intervals. A reverse filtration tube is arranged in the filtration tube placement area, and an outer sleeve tube is arranged in the to-be-treated sample area. The filtration unit is used to clamp the reverse filtration tube and insert it into the outer sleeve tube to filter the sample. The pipetting unit is used to suck the filtered sample in the outer sleeve tube and inject it into a test tube in the filtered sample area. The control unit is used to control the start of the filtration unit and the pipetting unit.

[0009] By adopting the above technical solution, during use, the staff places the clean reverse filtration tube into the filtration tube placement area, places the outer tube containing the sample mixture that has been added with sample extraction solution and vortex-treated into the area of the sample to be processed, places the clean centrifuge tube or test tube into the area of the filtered sample, and places the clean liquid suction pipette into the clean pipette storage area. Then, the filtration unit and the pipetting unit can be started through the control unit. Thus, the clean reverse filtration tube is clamped and inserted into the outer tube in the area of the sample to be processed through the filtration unit, so as to filter the sample mixture in the outer tube through the reverse filtration tube. The sample mixture in the outer tube enters the reverse filtration tube after filtration. Then, the pipetting unit is connected to the clean liquid suction pipette head to suck the filtered liquid in the reverse filtration tube in the area of the sample to be processed, and the sucked filtered liquid is discharged into the area of the filtered sample. Then, the used liquid suction pipette is moved into the waste area for recycling. The overall use process is simple and convenient. At the same time, rapid filtration can be achieved through the cooperation of multiple groups of the above units, which is beneficial to saving time and also achieves the purpose of high-throughput solid-liquid separation.

[0010] Preferably, the reverse filtration tube includes a tube body, a grid bottom plate arranged at the bottom end of the tube body, and a filter sieve plate. The grid bottom plate is provided with two layers, and the filter sieve plate is located between the two layers of grid bottom plates. A tube edge is fixed to the outer wall of the top end of the tube body. A positioning structure for positioning the tube body is arranged between the tube body and the outer tube.

[0011] By adopting the above technical solution, during use, the tube edge is provided to facilitate the staff to pick up the tube body. Through the cooperation of the two layers of grid bottom plates and the filter sieve plate, it is beneficial to ensure the filtration effect on the sample mixture. With the cooperation of the positioning structure, it can be applied to filtration with different requirements.

[0012] Preferably, a plurality of the reverse filtration tubes and outer tubes are provided. The plurality of reverse filtration tubes are distributed in a rectangular array. A connecting plate is arranged between the plurality of reverse filtration tubes, and the connecting plate connects the tops of the plurality of reverse filtration tubes together. The outer tubes are arranged in one-to-one correspondence with the reverse filtration tubes. A tube rack is arranged between the plurality of outer tubes, and the tube rack fixes the plurality of outer tubes together.

[0013] By adopting the above technical solution, during use, the plurality of reverse filtration tubes are connected into a whole through the connecting plate, and the plurality of outer tubes are connected into a whole through the tube rack. Thus, on the premise of ensuring the supporting use of the reverse filtration tubes and the outer tubes, the sample volume filtered by the device at one time is increased, thereby improving the filtration efficiency and also saving time.

[0014] Preferably, a first conveyor belt, a second conveyor belt, and a third conveyor belt are arranged at intervals on the sample loading platform. The conveying directions of the first conveyor belt, the second conveyor belt, and the third conveyor belt are parallel to the width direction of the machine base. A plurality of filter tube placement areas, areas for samples to be processed, areas for filtered samples, and clean pipette tip storage areas are provided. The plurality of filter tube placement areas are arranged at intervals and evenly along the conveying direction of the first conveyor belt on the first conveyor belt. The plurality of areas for samples to be processed and areas for filtered samples are arranged at intervals and evenly along the conveying direction of the second conveyor belt on the second conveyor belt. The plurality of clean pipette tip storage areas are arranged at intervals and evenly along the conveying direction of the third conveyor belt on the third conveyor belt. The conveying directions of the first conveyor belt and the third conveyor belt are opposite. A positioning unit for positioning the first conveyor belt, the second conveyor belt, and the third conveyor belt is further provided on the machine base.

[0015] By adopting the above technical solution, during use, through the coordinated arrangement of a plurality of filter tube placement areas, areas for samples to be processed, areas for filtered samples, and clean pipette tip storage areas, and in combination with the first conveyor belt, the second conveyor belt, the third conveyor belt, and the positioning unit, the automation of the sample pretreatment process is realized. The staff only needs to replace clean experimental instruments, which is simple and convenient to use.

[0016] Preferably, the positioning unit includes positioning protrusions arranged in the filter tube placement areas, areas for samples to be processed, areas for filtered samples, and clean pipette tip storage areas, and positioning sensors arranged in the filtering unit and the pipetting unit. The positioning sensors are arranged in cooperation with the positioning protrusions.

[0017] By adopting the above technical solution, during use, through the cooperation of the positioning protrusions and the positioning sensors, the transmission of the first conveyor belt, the second conveyor belt, and the third conveyor belt is positioned, so as to ensure that the transmission positions of the filter tube placement areas, areas for samples to be processed, areas for filtered samples, and clean pipette tip storage areas correspond one by one, thereby ensuring the normal progress of the experimental process.

[0018] Preferably, the filtering unit includes a first sliding seat slidably arranged on the machine base along the length direction of the machine base, a first driving component arranged on the machine base for driving the first sliding seat to slide, a second sliding seat slidably arranged on the first sliding seat along the height direction of the machine base, a clamping jaw arranged on the second sliding seat, and a second driving component arranged on the first sliding seat for driving the clamping jaw to lift and lower. The clamping jaw is used for clamping the reverse filter tube.

[0019] By adopting the above technical solution, during use, through the combined use of the first driving component and the second driving component, the purpose of driving the clamping jaw to move and lift is realized, so as to realize clamping and moving the reverse filter tube to be connected to the outer tube in the area for samples to be processed, which is simple and convenient to use.

[0020] Preferably, the pipetting unit includes a third sliding seat slidably disposed on the machine base along the length direction of the machine base, a third driving assembly disposed on the machine base for driving the third sliding seat to slide, a fourth sliding seat slidably disposed on the third sliding seat along the height direction of the machine base, a fourth driving assembly disposed on the third sliding seat for driving the fourth sliding seat to slide, a liquid suction tube head disposed on the fourth sliding seat, a liquid extraction pump disposed on the machine base, and an infusion hose for connecting the liquid extraction pump and the liquid suction tube head. The liquid suction tube head is inserted and matched with the liquid extraction pipette in the clean pipette storage area. A pipette removal assembly for removing the liquid extraction pipette connected to the liquid suction tube head is further disposed on the fourth sliding seat.

[0021] By adopting the above technical solution, during use, through the combined use of the third driving assembly and the fourth driving assembly, the liquid suction tube head is driven to move between the sample area to be processed, the filtered sample area, the clean pipette storage area, and the waste area. Thus, the liquid suction tube head is connected to the clean liquid extraction pipette, and then through the cooperation of the liquid extraction pump and the infusion hose, the filtered liquid in the sample area to be processed is sucked and transferred to the filtered sample area. When moving above the waste area, the used liquid extraction pipette can be removed from the liquid suction tube head through the pipette removal assembly, and the used liquid extraction pipette is collected through the waste area, thereby completing the sample pretreatment operation. The overall process is simple and convenient, and the quality of sample processing is ensured.

[0022] Preferably, the pipette removal assembly includes a first servo motor installed on the fourth sliding seat, a first lead screw rotatably disposed on the fourth sliding seat, a slider slidably disposed along the height direction of the machine base, a push rod fixed to the slider, a contact plate disposed at the end of the push rod away from the slider, and a return spring disposed between the contact plate and the fourth sliding seat. A guide post is disposed on the contact plate, and the guide post is slidably inserted and matched with the fourth sliding seat. The return spring is sleeved on the guide post, and one end of the return spring is fixedly connected to the contact plate, and the other end of the return spring is fixedly connected to the fourth sliding seat. The bottom end of the liquid suction tube head passes through the contact plate and is inserted and matched with the liquid extraction pipette in the clean pipette storage area, and the contact plate is in contact with the top end of the liquid extraction pipette.

[0023] By adopting the above technical solution, during use, when it is necessary to remove the liquid extraction pipette from the liquid suction tube head, the output shaft of the first servo motor rotates forward to drive the first lead screw to rotate, so that the slider drives the push rod to move downward, thereby driving the contact plate to move downward. Then, through the contact of the contact plate with the liquid extraction pipette, the purpose of removing the liquid extraction pipette from the liquid suction tube head can be achieved. When the output shaft of the first servo motor rotates in the reverse direction to drive the push rod to reset, under the pulling force of the return spring, the contact plate slides upward to reset, and the overall use is simple and convenient.

[0024] Preferably, the liquid extraction pump includes a plunger pump cylinder fixed on the machine base, a piston slidably disposed in the inner cavity of the plunger pump cylinder, a connecting rod fixed on the piston, a push plate fixed at the end of the connecting rod away from the piston, and a fifth driving assembly disposed on the machine base for driving the push plate to slide. The liquid outlet of the plunger pump cylinder is connected to an infusion hose.

[0025] By adopting the above technical solution, during use, the push plate is driven to slide by the fifth driving assembly, so as to drive the connecting rod and the piston to move in the inner cavity of the plunger pump cylinder, and then in cooperation with the infusion hose and the liquid suction tube head, the suction and discharge of the sample filtrate can be realized.

[0026] In a second aspect, the present application provides a filtering method for biological sample detection, adopting the following technical solution: A filtering method for biological sample detection includes the following steps: S1. Place a clean reverse filtering tube in the filtering tube placement area, place the outer sleeve in the area of the sample to be processed, and load the sample mixture that has been added with the sample extraction solution and subjected to vortex treatment into the outer sleeve. Place a clean centrifuge tube in the filtered sample area, and place a clean liquid extraction pipette tip in the clean pipette tip storage area; S2. Control the first conveyor belt, the second conveyor belt, and the third conveyor belt to drive. Under the cooperation of the positioning protrusion and the positioning sensor, the first conveyor belt, the second conveyor belt, and the third conveyor belt pause driving after driving to the designated position; S3. Under the action of the second driving assembly, the clamping jaws move downward and clamp the connecting plate, and synchronously take out several reverse filtering tubes. Under the action of the first driving assembly, move the reverse filtering tubes above the outer sleeve in the area of the sample to be processed, and insert the reverse filtering tubes into the outer sleeve, so that the sample mixture in the outer sleeve enters the reverse filtering tube after filtration, and then control the clamping jaws to move out of the area of the sample to be processed; S4. Through the cooperation of the third driving assembly and the fourth driving assembly, align the liquid suction tube head with the liquid extraction pipette tip in the clean pipette tip storage area, and sleeved the liquid extraction pipette tip on the liquid suction tube head; S5. Control the liquid suction tube head with the liquid extraction pipette tip to move above the reverse filtering tube in the area of the sample to be processed, control the liquid extraction pipette tip to extend into the reverse filtering tube, and suck the filtrate through the liquid extraction pump; S6. Control the liquid extraction pipette tip that has sucked the filtrate to move above the centrifuge tube in the filtered sample area, so as to transfer the filtrate into the centrifuge tube; S7. Control the liquid suction tube head to move the used liquid extraction pipette tip above the clean pipette tip storage area, and remove the liquid extraction pipette tip from the liquid suction tube head through the pipette tip removal assembly. The used liquid extraction pipette tip falls into the clean pipette tip storage area to complete the recovery; S8. Control the first conveyor belt, the second conveyor belt, and the third conveyor belt to continue to move to the next position, repeat the above steps for a new round of sample filtration, and the staff can take the filtered sample from the filtered sample area.

[0027] By adopting the above technical solution, during use, through the cooperation of the first conveyor belt, the second conveyor belt, the third conveyor belt, the positioning protrusions and the positioning sensors, the filtering unit and the liquid transfer unit, the automated processing is realized during the pretreatment process of biological samples. The overall use is convenient and fast, which is conducive to saving processing time, and also realizes the purpose of high-throughput solid-liquid separation.

[0028] In summary, the present application includes at least one of the following beneficial technical effects: 1. During use, the staff only needs to place the clean reverse filtration tube in the filtration tube placement area, place the outer tube containing the sample mixture that has been added with the sample extraction solution and vortex-treated in the area of the sample to be processed, place the clean centrifuge tube in the filtered sample area, and place the clean liquid extraction pipette tip in the clean pipette tip storage area. Then, the filtration unit and the liquid transfer unit can be started through the control unit to achieve the purpose of filtering, collecting the liquid of the sample mixture. The overall use process is simple and convenient, and at the same time, rapid filtration is realized, which is conducive to saving time, and also realizes the purpose of high-throughput solid-liquid separation; 2. Through the setting of the two-layer grid bottom plate and the filter sieve plate, the effect of filtering the sample mixture is ensured, thereby ensuring the processing effect of the sample pretreatment link; 3. Through the coordinated use of the first conveyor belt, the second conveyor belt, the third conveyor belt, and the positioning unit, the liquid extraction process of the whole device is automated, thereby improving the processing efficiency of the sample pretreatment process and being conducive to saving time. Description of the Drawings

[0029] Figure 1 is an isometric schematic diagram mainly showing the overall structure in the embodiment of the present application; Figure 2 is an isometric schematic diagram mainly showing the structure of the machine base in the embodiment of the present application; Figure 3 is an isometric schematic diagram mainly showing the structure of the sample loading platform in the embodiment of the present application; Figure 4 is a sectional view mainly showing the structure of the reverse filtration tube in the embodiment of the present application; Figure 5 is an exploded view mainly showing the structure of the reverse filtration tube in the embodiment of the present application; Figure 6 is an exploded view mainly showing the combined structure of the reverse filtration tubes in the embodiment of the present application; Figure 7 is an isometric schematic diagram mainly showing the structure of the filtering unit in the embodiment of the present application; Figure 8 It is an axonometric schematic diagram mainly showing the structure of the pipetting unit in the embodiment of the present application; Figure 9 It is an axonometric schematic diagram mainly showing the structure of the tip retraction assembly in the embodiment of the present application; Figure 10 It is an axonometric schematic diagram mainly showing the structure of the liquid extraction pump in the embodiment of the present application.

[0030] Reference numerals: 1, machine base; 11, lower base body; 12, column; 13, cross beam; 2, sample stage; 21, filter tube placement area; 22, sample to be processed area; 23, filtered sample area; 24, clean tip storage area; 25, waste area; 26, first conveyor belt; 27, second conveyor belt; 28, third conveyor belt; 3, filtration unit; 31, first sliding seat; 32, first driving assembly; 33, second sliding seat; 34, clamping jaw; 35, second driving assembly; 4, pipetting unit; 41, third sliding seat; 42, third driving assembly; 43, fourth sliding seat; 44, fourth driving assembly; 441, second servo motor; 442, second lead screw; 443, guide rail; 45, liquid suction pipe head; 46, liquid extraction pump; 461, plunger pump cylinder body; 462, piston; 463, connecting rod; 464, push plate; 465, fifth driving assembly; 47, infusion hose; 48, tip retraction assembly; 481, first servo motor; 482, first lead screw; 483, slider; 484, push rod; 485, abutting plate; 486, return spring; 487, guide post; 5, control unit; 51, control panel; 6, reverse filter tube; 61, tube body; 62, grid bottom plate; 63, filter screen plate; 64, tube edge; 7, outer sleeve tube; 8, connecting plate; 9, tube rack; 10, positioning unit; 101, positioning protrusion; 102, positioning sensor; 20, collection box. Detailed implementation manners

[0031] The following will further describe the present application in detail with reference to the attached Figure 1 - attached Figure 10 drawings.

[0032] The embodiment of the present application discloses a filtering device and a filtering method for biological sample detection.

[0033] Refer to Figure 1 and Figure 2, a filtering device for biological sample detection, comprising a horizontally placed base 1, a sample stage 2, a filtering unit 3, a pipetting unit 4 and a control unit 5. Among them, the base 1 includes a lower base body 11, columns 12 and a cross beam 13. There are two columns 12, which are symmetrically arranged at both ends of the lower base body 11 in the length direction. The cross beam 13 is located between the two columns 12, and one end of the cross beam 13 is welded and fixed to the side wall of the top of one column 12, and the other end is welded and fixed to the side wall of the top of the other column 12.

[0034] Refer to Figure 1 and Figure 3 , the sample stage 2 is fixed to the lower base body 11 by bolts, and the sample stage 2 is located between the two columns 12. On the sample stage 2, a filter tube placement area 21, a sample to be processed area 22, a filtered sample area 23, a waste area 25, and a clean pipette tip storage area 24 are sequentially arranged at intervals from left to right. A clean reverse filter tube 6 is placed in the filter tube placement area 21, and a jacket tube 7 used in cooperation with the reverse filter tube 6 is placed in the sample to be processed area 22. The filtered sample area 23 is used to place a centrifuge tube storage rack containing clean centrifuge tubes, and the clean pipette tip storage area 24 is used to place a pipette tip box containing clean liquid-taking pipette tips.

[0035] Refer to Figure 1 and Figure 3 , on the sample stage 2, a first conveyor belt 26, a second conveyor belt 27 and a third conveyor belt 28 are arranged at intervals along its own length direction. The conveying directions of the first conveyor belt 26, the second conveyor belt 27 and the third conveyor belt 28 are parallel to the width direction of the sample stage 2, and the first conveyor belt 26, the second conveyor belt 27 and the third conveyor belt 28 are all controlled to drive by a separate servo motor. The filter tube placement area 21 is arranged on the first conveyor belt 26, the sample to be processed area 22 and the filtered sample area 23 are arranged on the second conveyor belt 27, and the clean pipette tip storage area 24 is arranged on the third conveyor belt 28. In this embodiment, the conveying direction of the third conveyor belt 28 is opposite to the conveying direction of the first conveyor belt 26, and the conveying directions of the first conveyor belt 26 and the second conveyor belt 27 are the same.

[0036] Refer to Figure 1 and Figure 3, In addition, in the present application, there are several filter tube placement areas 21, areas 22 for samples to be processed, areas 23 for filtered samples, and clean pipette tip storage areas 24. The several filter tube placement areas 21 are evenly distributed at intervals along the conveying direction of the first conveyor belt 26. The several areas 22 for samples to be processed are evenly distributed at intervals along the conveying direction of the second conveyor belt 27. The several areas 23 for filtered samples are distributed in one-to-one correspondence with the several areas 22 for samples to be processed. The clean pipette tip storage areas 24 are evenly distributed at intervals along the conveying direction of the third conveyor belt 28. At the same time, there is also a positioning unit 10 on the machine base 1. The positioning unit 10 is used to position the first conveyor belt 26, the second conveyor belt 27, and the third conveyor belt 28.

[0037] Refer to Figure 1 and Figure 3 , The positioning unit 10 includes positioning protrusions 101 and positioning sensors 102. There are several groups of positioning protrusions 101. The several groups of positioning protrusions 101 are correspondingly arranged with the filter tube placement areas 21, the areas 22 for samples to be processed, the areas 23 for filtered samples, and the clean pipette tip storage areas 24. That is, one group of positioning protrusions 101 is arranged in each of the filter tube placement areas 21, the areas 22 for samples to be processed, the areas 23 for filtered samples, and the clean pipette tip storage areas 24. Each group of positioning protrusions 101 includes two positioning protrusions 101. The two groups of positioning protrusions 101 are arranged at intervals. There are two groups of positioning sensors 102. One group of positioning sensors 102 cooperates with the filtering unit 3, and the other group of positioning sensors 102 cooperates with the pipetting unit 4. In this embodiment, one group of positioning sensors 102 also includes two positioning sensors 102, and the two positioning sensors 102 are used in cooperation with the two positioning protrusions 101 in one group of positioning protrusions 101.

[0038] Refer to Figure 1 and Figure 3 , During use, the staff only need to place the prepared experimental instruments corresponding to the above-mentioned filter tube placement areas 21, areas 22 for samples to be processed, areas 23 for filtered samples, and clean pipette tip storage areas 24. Then, the corresponding experimental instruments can be moved to the designated positions through the first conveyor belt 26, the second conveyor belt 27, and the third conveyor belt 28. That is, when both positioning sensors 102 detect different positioning protrusions 101 within the same group of positioning protrusions 101, the samples in the experimental instruments can be filtered, sampled, and pipetted through the filtering unit 3 and the pipetting unit 4. The overall process is automated. By adopting this operation mode and combining with other sample processing equipment, it can be used as a part of the automated sample processing and detection pipeline in an intelligent laboratory. The overall operation process is simple and convenient, and it is also beneficial to save time.

[0039] Refer to Figure 1 and Figure 4, the reverse filtration tube 6 includes two parts: a tube body 61 and a filtration layer. In this embodiment, the outer diameter of the tube body 61 is between 5 mm and 50 mm, preferably between 8 mm and 60 mm, including but not limited to different specifications such as 8 mm to 9.5 mm, 13 mm to 16 mm, 15 mm to 18 mm, 26 mm to 30 mm, etc. The height of the tube body 61 is between 15 mm and 200 mm, preferably between 20 mm and 150 mm. At the top end of the tube body 61, there is an integrally formed tube flange 64 with an outer diameter larger than that of the tube body 61. The outer diameter of the tube flange 64 is between 5 mm and 60 mm, preferably between 8.5 mm and 62 mm. The thickness of the tube flange 64 is between 1 mm and 5 mm, preferably between 1.5 mm and 3 mm. The height of the tube flange 64 is between 1 mm and 10 mm, preferably between 2 mm and 5 mm.

[0040] Refer to Figure 4 and Figure 5 , the lower end of the tube body 61 is a bottomless hollow structure, and the filtration layer is fixed at the position near the lowermost end inside the tube body 61, that is, there is a spacing between the filtration layer and the bottom end of the tube body 61. In this embodiment, the set height of the filtration layer is 1 cm to 5 cm from the lowermost end of the tube body 61, preferably the set height of the filtration layer is 1.5 cm to 3 cm from the lowermost end of the tube body 61. The filtration layer includes two layers of grid bottom plates 62 and one layer of filtration sieve plate 63. The pore size of the grid bottom plate 62 is between 20 mesh and 400 mesh, preferably between 50 mesh and 170 mesh. The gap between the two layers of grid bottom plates 62 is between 1 mm and 10 mm, preferably between 1.5 mm and 4 mm. The filtration sieve plate 63 is located between the two layers of grid bottom plates 62. The pore size of the filtration sieve plate 63 is between 2 μm and 100 μm, preferably between 10 μm and 50 μm. The thickness of the filtration sieve plate 63 is between 1 mm and 10 mm, preferably between 1.5 mm and 4 mm.

[0041] Refer to Figure 4 and Figure 5 , in this embodiment, one layer of grid bottom plate 62 above the filtration sieve plate 63 is preferably made of polystyrene with a mesh size of 50 - 170 mesh. The layer of grid bottom plate 62 below the filtration sieve plate 63 is also made of polystyrene with a mesh size of 50 - 170 mesh. The lower grid bottom plate 62 and the upper grid bottom plate 62 can be set to the same mesh number or different mesh numbers as needed, and the distance between the lowermost grid bottom plate 62 and the lowermost end of the tube body 61 is preferably set to 2 cm.

[0042] Refer to Figure 1 and Figure 4, during use, the above-mentioned reverse filtration tube 6 can be used in conjunction with a common laboratory centrifuge tube. That is, the centrifuge tube is placed on the corresponding centrifuge tube storage rack. After crushing the biological sample to be detected and adding it to the centrifuge tube, and adding the sample extraction solution to be detected, after vortex mixing, the reverse filtration tube 6 matching the centrifuge tube is placed into the centrifuge tube, and it is pressed down so that the bottom of the reverse filtration tube 6 drops to the solid layer of the sample mixture in the centrifuge tube. At this time, the liquid part of the extraction solution enters the reverse filtration tube 6 through the filter layer in the reverse filtration tube 6, while the solid impurities remain in the centrifuge tube. Then, a certain amount of filtered liquid can be aspirated from the reverse filtration tube 6 for subsequent operations.

[0043] Refer to Figure 1 and Figure 4 , the outer sleeve 7 can also be used in combination with the reverse filtration tube 6. The bottom of the outer sleeve 7 can be set to a pointed bottom, a round bottom or a flat bottom. In this embodiment, it is preferably set to a flat bottom. The inner diameter of the outer sleeve 7 is between 5 mm and 50 mm, preferably between 8 mm and 60 mm. The height of the outer sleeve 7 is lower than the height of the reverse filtration tube 6, and the height difference between the two is between 2 mm and 20 mm, preferably between 5 mm and 15 mm.

[0044] Refer to Figure 1 and Figure 4 , when the above-mentioned reverse filtration tube 6 and the outer sleeve 7 are used in combination, the staff puts the crushed biological sample into the outer sleeve 7, adds the sample extraction solution, vortex mixes it, then puts the reverse filtration tube 6 into the outer sleeve 7, and presses the reverse filtration tube 6 to the bottom. Then, a fixed volume of filtered sample can be aspirated from the reverse filtration tube 6 for subsequent processing.

[0045] Refer to Figure 1 and Figure 4 , when the above-mentioned reverse filtration tube 6 and the outer sleeve 7 are used in combination, the solid is left in the outer sleeve 7, and the filtered liquid enters the reverse filtration tube 6. This filtration method does not require external forces such as a centrifuge, and the reverse filtration tube 6 can complete the filtration by itself, simplifying the solid-liquid separation process; at the same time, it can very cleanly separate the solid phase and the liquid phase, avoiding the problem that the precipitation is unstable during the centrifugation process, and the precipitation part dissolves to form turbidity during subsequent operations, thus affecting the subsequent detection steps; furthermore, it ensures the accuracy of the detection and reduces the error caused by incomplete solid-liquid separation during the sample pretreatment process.

[0046] Refer to Figure 1 and Figure 6, in order to improve the overall detection efficiency and increase the throughput of sample processing, in this application, a plurality of reverse filtration tubes 6 and outer tubes 7 are provided. The plurality of reverse filtration tubes 6 are distributed in a rectangular array. The positions of the plurality of outer tubes 7 are adapted to the reverse filtration tubes 6. A connecting plate 8 is also provided between the plurality of reverse filtration tubes 6. The connecting plate 8 fixedly connects the tops of the plurality of reverse filtration tubes 6 together, so that the plurality of reverse filtration tubes 6 form a whole. Similarly, a pipe rack 9 is also provided between the outer tubes 7. The pipe rack 9 combines the plurality of outer tubes 7 into a whole.

[0047] Referring to Figure 1 and Figure 6 , in this application, the reverse filtration tubes 6 can be set in a variety of specifications according to actual usage requirements, such as 16 holes, 24 holes, 32 holes, 48 holes, etc. In this embodiment, 48 holes are taken as an example for description. When in use, 48 reverse filtration tubes 6 can be a whole, or can adopt a multi-group split combination form of a group of 4 reverse filtration tubes 6 or a group of 8 reverse filtration tubes 6. The setting form of the outer tube 7 matches that of the reverse filtration tube 6; the height difference between the above-mentioned outer tube 7 and the reverse filtration tube 6 is between 5 mm and 20 mm, preferably 5 mm to 15 mm, and the width and length of the connecting plate 8 both exceed those of the pipe rack 9, and the width difference and length difference between the two are between 5 mm and 20 mm, preferably 5 mm - 10 mm.

[0048] Referring to Figure 1 and Figure 6 , when in use, put the reverse filtration tubes 6 combined into a whole into the filter tube placement area 21, place the outer tubes 7 combined into a whole in the area of the sample to be processed 22, weigh 48 different samples to be processed, and place each sample in an independent outer tube 7, add the extraction solution respectively, then put a cover plate on the outer tube 7, after vortex oscillation, take the reverse filtration tubes 6 combined into a whole, place them on the above-mentioned outer tube 7 according to the positioning, press down the reverse filtration tubes 6, and the sample processing solution filters into the reverse filtration tubes 6 after passing through, and the solid residue remains in the outer tube 7, so as to realize the filtration operation of multiple samples synchronously and improve the overall processing efficiency.

[0049] Referring to Figure 1 and Figure 7, the filtering unit 3 includes a first sliding seat 31, a first driving component 32, a second sliding seat 33, a clamping jaw 34 and a second driving component 35. The first sliding seat 31 is slidably connected to the cross beam 13 and slides along the length direction of the cross beam 13. The first driving component 32 is arranged at one end of the cross beam 13 and is used to drive the cross beam 13 to slide. The second sliding seat 33 is slidably connected to the first sliding seat 31 and slides in the vertical direction. The second driving component 35 is arranged on the first sliding seat 31 and is used to drive the second sliding seat 33 to move up and down. The clamping jaw 34 is installed on the second sliding seat 33 and is used to clamp the connecting plate 8 or a single reverse filtering tube 6. In this embodiment, the clamping jaw 34 is used to clamp the connecting plate 8, and a group of positioning sensors 102 are arranged on the clamping jaw 34.

[0050] Refer to Figure 1 and Figure 7 , during use, first drive the first sliding seat 31 to move above the filter tube placement area 21 through the first driving component 32. When the positioning sensor 102 on the clamping jaw 34 detects the positioning protrusion 101 in the filter tube placement area 21, the reverse filtering tubes 6 combined into a whole in the filter tube placement area 21 are located directly below the clamping jaw 34. At this time, the clamping jaw 34 can be driven to move down through the second driving component 35, so as to clamp the connecting plate 8 through the clamping jaw 34 and take out the reverse filtering tube 6 in one of the filter tube placement areas 21. Then, through the first driving component 32, move the first sliding seat 31 above the sample area to be processed 22. When the positioning sensor 102 on the clamping jaw 34 detects the positioning protrusion 101 in the sample area to be processed 22, the clamping jaw 34 can be driven to descend through the second driving component 35 until the reverse filtering tube 6 is inserted into the corresponding outer sleeve 7, so as to filter the sample mixture in the outer sleeve 7 through the reverse filtering tube 6. Finally, control the clamping jaw 34 to release the connecting plate 8 and move away from the sample area to be processed 22.

[0051] Refer to Figure 1 and Figure 8, the pipetting unit 4 includes a third slide 41, a third driving assembly 42, a fourth slide 43, a fourth driving assembly 44, a pipette tip 45, a liquid extraction pump 46 and an infusion hose 47. Among them, the third slide 41 is slidably connected to the cross beam 13, and the sliding direction of the third slide 41 is parallel to the length direction of the cross beam 13. The third driving assembly 42 is arranged at one end of the cross beam 13 and is used to drive the third slide 41 to slide. The fourth slide 43 is slidably connected to the third slide 41 in the vertical direction. The fourth driving assembly 44 is arranged on the third slide 41 and is used to drive the fourth slide 43 to move up and down. The pipette tip 45 is installed on the fourth slide 43 and is in interference fit with the liquid extraction tip in the clean tip storage area 24. There are several pipette tips 45, and several pipette tips 45 are spaced along the length direction of the fourth slide 43. In this embodiment, the pipette tips 45 are preferably set to 8, and the positions of the 8 pipette tips 45 correspond one by one to the positions of a column of reverse filter tubes 6 arranged in the length direction of the connecting plate 8.

[0052] Referring to Figure 8 and Figure 9 , a tip ejection assembly 48 is further arranged on the fourth slide 43, and the tip ejection assembly 48 is used to eject the liquid extraction tip from the pipette tip 45. The corresponding positioning sensor 102 is arranged on the fourth slide 43. During use, first drive the third slide 41 to move through the third driving assembly 42 to drive the pipette tip 45 to move above the clean tip storage area 24. When the positioning sensor 102 detects the positioning protrusion 101 in the clean tip storage area 24, the pipette tip 45 is directly above the clean liquid extraction tip. At this time, the fourth driving assembly 44 can be used to drive the fourth slide 43 to descend, so that the pipette tip 45 gradually inserts into the liquid extraction tip to a certain depth. Then when the fourth slide 43 rises, under the friction force between the pipette tip 45 and the liquid extraction tip, the liquid extraction tip is connected to the pipette tip 45 and rises synchronously, thereby realizing the connection between the liquid extraction tip and the pipette tip 45. When it is necessary to separate the liquid extraction tip and the pipette tip 45, it can be realized through the tip ejection assembly 48.

[0053] Referring to Figure 8 and Figure 9, the desorption head assembly 48 includes a first servo motor 481, a first lead screw 482, a slider 483, a push rod 484, a contact plate 485 and a return spring 486. The first servo motor 481 is installed on the fourth slide 43, the first lead screw 482 rotates on the fourth slide 43, the output shaft of the first servo motor 481 is coaxially and fixedly connected to the first lead screw 482. The first lead screw 482 is vertically arranged, the slider 483 is threadedly connected to the first lead screw 482, the top end of the push rod 484 is fixed to the slider 483, the push rod 484 is slidably inserted and matched with the first lead screw 482. The bottom end of the push rod 484 passes through the fourth slide 43 and abuts against the contact plate 485. A guide post 487 is arranged on the contact plate 485, the guide post 487 is slidably inserted and matched with the fourth slide 43. The return spring 486 is sleeved on the guide post 487, and one end of the return spring 486 is fixedly connected to the bottom end of the fourth slide 43, and the other end of the return spring 486 is fixedly connected to the contact plate 485. The bottom end of the liquid suction tube head 45 passes through the contact plate 485 and is connected to the liquid extraction pipette. The positioning sensor 102 is located on the bottom end surface of the contact plate 485.

[0054] Referring to Figure 8 and Figure 10 , the liquid extraction pump 46 is installed on the column 12 on one side. The liquid extraction pump 46 includes a piston pump body 461, a piston 462, a connecting rod 463, a push plate 464 and a fifth driving assembly 465. In this application, the piston pump body 461, the infusion hose 47 and the liquid suction tube head 45 are provided in the same number and are arranged in one-to-one correspondence, that is, both the piston pump body 461 and the infusion hose 47 are provided with 8. The number of the pistons 462 and the connecting rods 463 is the same as that of the piston pump body 461 and is arranged in one-to-one correspondence. The piston pump body 461 is installed on the column 12, the piston 462 is slidably arranged in the inner cavity of the piston pump body 461. One end of the connecting rod 463 is integrally formed with the rod of the piston 462, and the other end is fixedly connected to the push plate 464. The push plate 464 is slidably arranged along the vertical direction on the vertical rod. The fifth driving assembly 465 is used to drive the push plate 464 to move. One end of the infusion hose 47 is communicated with the liquid outlet of the piston pump body 461, and the other end is communicated with the liquid suction tube head 45.

[0055] Referring to Figure 8 and Figure 10, during use, the third slider 41 is driven to slide by the third driving component 42, and the liquid suction tube head 45 connected with the liquid extraction pipette is moved to the area 22 of the sample to be processed. The positioning sensor 102 on the abutting plate 485 detects the positioning protrusion 101 in the area 22 of the sample to be processed, so that the liquid extraction pipette is aligned with the reverse filtration tube 6. Then, the fourth slider 43 descends to insert the liquid extraction pipette into the reverse filtration tube 6. After that, the fifth driving component 465 drives the push plate 464 to move upward, so as to drive 8 pistons 462 to move upward synchronously, thereby sucking the sample filtrate in the reverse filtration tube 6 into the liquid extraction pipette. Then, through the cooperation of the third driving component 42 and the fourth driving component 44, the liquid extraction pipette is moved above the area 23 of the filtered sample until the positioning sensor 102 on the abutting plate 485 detects the positioning protrusion 101 in the area 23 of the filtered sample, and then the liquid extraction pipette can be inserted into the centrifuge tube or test tube. Thus, the fifth driving component 465 drives the push plate 464 to descend to discharge the filtrate in the liquid extraction pipette into the centrifuge tube or test tube. Finally, the third driving component 42 drives the used liquid extraction pipette to move above the waste area 25, and the pipette withdrawal component 48 withdraws the liquid extraction pipette from the liquid suction tube head 45, so that the liquid extraction pipette falls into the waste area 25.

[0056] Refer to Figure 1 and Figure 9 , in this application, the implementation structures of the first driving component 32, the second driving component 35, the third driving component 42, the fourth driving component 44 and the fifth driving component 465 are the same, and all adopt the method of controlling the rotation of the lead screw by a servo motor to realize the movement of the corresponding module. Taking the fourth driving component 44 as an example, the fourth driving component 44 includes a second servo motor 441, a second lead screw 442 and a guide rail 443. The second servo motor 441 is installed on the third slider 41, the second lead screw 442 is rotatably arranged on the third slider 41, the second lead screw 442 is vertically arranged, the guide rail 443 is fixed on the third slider 41, and the fourth slider 43 is slidably matched with the guide rail 443; during use, the output shaft of the second servo motor 441 rotates to drive the second lead screw 442 to rotate, so as to realize the purpose of driving the fourth slider 43 to lift and lower.

[0057] Refer to Figure 1 , in this application, a collection box 20 is arranged below the waste area 25, and the waste area 25 is arranged in a through manner, that is, after the liquid extraction pipette falls into the waste area 25, it finally falls into the collection box 20, so as to recycle the used liquid extraction pipette through the collection box 20, which is more convenient to use.

[0058] Refer to Figure 1 and Figure 2, the control unit 5 includes, but is not limited to, algorithm software installed on a tablet computer or a desktop computer, as well as circuits, sensors, and solenoid valve systems at all levels. The software controls the start, stop, movement, and parameters such as rotational speed, temperature, and residence time of the corresponding servo motors in the overall device through the circuits, sensors, etc., so as to achieve the purpose of controlling the entire filtration system; at the same time, the control unit 5 realizes the setting of various parameters through the control panel 51 to achieve the control function.

[0059] A filtration method for biological sample detection includes the following steps: S1. The experimenter places a clean reverse filtration tube 6 in the filtration tube placement area 21, places an outer sleeve tube 7 in the sample to be processed area 22, and loads a sample mixture that has been added with sample extraction solution and vortex-treated into the outer sleeve tube 7. A clean centrifuge tube is placed in the filtered sample area 23, a pipette tip box is placed in the clean pipette tip storage area 24, and a plurality of clean liquid-taking pipette tips are installed in the pipette tip box; S2. The control unit 5 controls the transmission of the first conveyor belt 26, the second conveyor belt 27, and the third conveyor belt 28. With the cooperation of the positioning protrusion 101 and the positioning sensor 102, the first conveyor belt 26, the second conveyor belt 27, and the third conveyor belt 28 stop transmitting after being driven to the designated position. At this time, the first group of reverse filtration tubes 6, outer sleeve tubes 7, centrifuge tubes, and liquid-taking pipette tips are located in the corresponding working areas below the filtration unit 3 and the liquid transfer unit 4; S3. Under the action of the second driving component 35, the clamping jaw 34 moves downward and clamps the connecting plate 8, and synchronously takes out several reverse filtration tubes 6. Under the action of the first driving component 32, in cooperation with the corresponding positioning protrusion 101 and the positioning sensor 102 in the sample to be processed area 22, several reverse filtration tubes 6 are moved above the outer sleeve tube 7 in the sample to be processed area 22, and by controlling the second sliding seat 33 to descend, the reverse filtration tubes 6 are inserted into the outer sleeve tube 7. Thus, during the downward pressing process of the reverse filtration tubes 6, the sample mixture in the outer sleeve tube 7 is filtered and enters the reverse filtration tubes 6, and the solids in the outer sleeve tube 7 remain in the outer sleeve tube 7. Then, the control unit controls the clamping jaw 34 to release the connecting plate 8 and moves out of the sample to be processed area 22, waiting for the next clamping operation; S4. Through the cooperation of the third driving component 42 and the fourth driving component 44, the liquid suction tube head 45 is moved above the clean pipette tip storage area 24. In cooperation with the corresponding positioning sensor 102 and the positioning protrusion 101 in the clean pipette tip storage area 24, the liquid suction tube head 45 is aligned with the liquid-taking pipette tip below. Subsequently, through the descent of the fourth sliding seat 43, the liquid suction tube head 45 is gradually inserted into the liquid-taking pipette tip, and the liquid-taking pipette tip is sleeved on the liquid suction tube head 45; S5. By controlling the upward movement of the fourth slide 43, the pipette tip head 45 with the liquid extraction pipette tip is moved above the reverse filtration tube 6 in the sample area to be processed 22. In cooperation with the corresponding positioning sensor 102 and the positioning protrusion 101 in the sample area to be processed 22, the liquid extraction pipette tip is aligned with the reverse filtration tube 6 below. Then, the liquid extraction pipette tip is controlled to descend into the reverse filtration tube 6, so that the filtration liquid in 8 reverse filtration tubes 6 is simultaneously sucked into the corresponding liquid extraction pipette tip through the liquid extraction pump 46; S6. Control the liquid extraction pipette tip with the sucked filtration liquid to move above the filtered sample area 23, and through the cooperation of the positioning sensor 102 on the fourth slide 43 and the positioning protrusion 101 in the filtered sample area 23, align the liquid extraction pipette tip with the centrifuge tube. Then, drive the liquid extraction pipette tip to insert into the centrifuge tube by the downward movement of the fourth slide 43, so that the filtration liquid is transferred into the centrifuge tube through the liquid extraction pump 46, thereby completing the filtration and liquid transfer process in one sample pretreatment; S7. Control the pipette tip head 45 to move the used liquid extraction pipette tip above the clean pipette tip storage area 24, and remove the liquid extraction pipette tip from the pipette tip head 45 through the pipette tip withdrawal assembly 48. The used liquid extraction pipette tip falls into the clean pipette tip storage area 24 and finally falls into the collection box 20 to complete the recycling; S8. Control the first conveyor belt 26, the second conveyor belt 27, and the third conveyor belt 28 to continue to drive to the next position, and repeat the above steps for a new round of sample filtration and liquid transfer. The staff only needs to take the filtered sample from the filtered sample area 23 and timely supplement the new reverse filtration tube 6 to the filtration tube placement area 21, supplement the outer tube 7 filled with the sample mixture to the sample area to be processed 22, and supplement the new centrifuge tube to the filtered sample area 23.

[0060] The implementation principle of the embodiment of this application is as follows: During use, the staff loads the sample mixture to be processed into the outer sleeve 7, places the outer sleeve 7 in the sample area 22 to be processed on the second conveyor belt 27, places the clean reverse filtration tube 6 of the corresponding specification in the filtration tube storage area on the first conveyor belt 26, places the clean centrifuge tube of the corresponding specification on the centrifuge tube storage rack in the filtered sample area 23 on the second conveyor belt 27, and places the clean pipette tip of the corresponding specification in the clean pipette tip storage area 24 on the third conveyor belt 28. Then, the corresponding parameters are adjusted through the control panel 51 to control the operation of the overall device. Under the action of the filtration unit 3, the sample mixture is filtered by inserting the reverse filtration tube 6 into the outer sleeve 7. Under the action of the liquid transfer unit 4, a clean liquid-taking pipette tip is installed on the liquid suction tube head 45, and the filtered liquid in the reverse filtration tube 6 is sucked through the liquid-taking pump 46 and transferred into the centrifuge tube. Then, the used liquid-taking pipette tip is withdrawn from the liquid suction tube head 45 through the pipette tip withdrawal assembly 48, thus completing the filtration and liquid transfer of the sample mixture. The overall processing process is automated, with simple and convenient operation. When used for solid-liquid separation during the sample pretreatment process, it avoids long-term centrifugation, greatly saves operation time, especially when multiple-step centrifugation is involved, and will greatly improve the separation efficiency.

[0061] The above are all the preferred embodiments of this application. Without restricting the protection scope of this application based on this, therefore: All equivalent changes made according to the structure, shape, and principle of this application shall be covered within the protection scope of this application.

Claims

1. A filtering device for biological sample detection, characterized in that: The invention comprises a machine base (1), a sample loading platform (2) arranged on the machine base (1), a filtering unit (3), a liquid transfer unit (4) and a control unit (5); the sample loading platform (2) is provided with a filter tube placement area (21), a sample area to be processed (22), a sample area after filtration (23), a clean pipette tip storage area (24) and a waste area (25) at intervals; a reverse filter tube (6) is arranged in the filter tube placement area (21); an outer sleeve (7) is arranged in the sample area to be processed (22); the filtering unit (3) is used to clamp the reverse filter tube (6) and insert it into the outer sleeve (7) to filter the sample; the liquid transfer unit (4) is used to absorb the sample after filtration in the outer sleeve (7) and inject it into the test tube in the sample area after filtration (23); and the control unit (5) is used to control the start of the filtering unit (3) and the liquid transfer unit (4).

2. A filtering device for biological sample detection according to claim 1, characterized in that: The reverse filtering tube (6) comprises a tube body (61), a grid bottom plate (62) arranged inside the tube body (61), and a filtering screen plate (63); the grid bottom plate (62) is provided with two layers, the filtering screen plate (63) is located between the two layers of grid bottom plates (62), and a tube edge (64) is fixed to the outer wall of the top end of the tube body (61).

3. A filtering device for biological sample detection according to claim 1, characterized in that: A plurality of the reverse filter tubes (6) and the outer sleeve (7) are provided, and the plurality of the reverse filter tubes (6) are distributed in a rectangular array. A connecting plate (8) is provided between the plurality of the reverse filter tubes (6), and the connecting plate (8) connects the top ends of the plurality of the reverse filter tubes (6) together. The outer sleeves (7) are provided in one-to-one correspondence with the reverse filter tubes (6), and a pipe rack (9) is provided between the plurality of the outer sleeves (7), and the pipe rack (9) fixes the plurality of the outer sleeves (7) together.

4. A filtering device for biological sample detection according to claim 1, characterized in that: A first conveyor belt (26), a second conveyor belt (27) and a third conveyor belt (28) are arranged at intervals on the sample loading platform (2); the conveying directions of the first conveyor belt (26), the second conveyor belt (27) and the third conveyor belt (28) are parallel to the width direction of the machine base (1); a plurality of filter tube placement areas (21), a sample area to be processed (22), a filtered sample area (23) and a clean suction head storage area (24) are arranged; and the plurality of filter tube placement areas (21) are evenly arranged on the first conveyor belt (26) along the conveying direction of the first conveyor belt (26). A plurality of the sample areas (22) to be processed and the sample areas (23) after filtration are evenly arranged on the second conveyor belt (27) along the conveying direction of the second conveyor belt (27); a plurality of the clean suction head storage areas (24) are evenly arranged on the third conveyor belt (28) along the conveying direction of the third conveyor belt (28); the conveying directions of the first conveyor belt (26) and the third conveyor belt (28) are opposite; and a positioning unit (10) for positioning the first conveyor belt (26), the second conveyor belt (27) and the third conveyor belt (28) is also arranged on the machine base (1).

5. A filtering device for biological sample detection according to claim 4, characterized in that: The positioning unit (10) comprises positioning protrusions (101) arranged in a filter tube placement area (21), a sample area to be processed (22), a filtered sample area (23), and a clean pipette tip storage area (24), and positioning sensors (102) arranged in the filter unit (3) and the pipetting unit (4), wherein the positioning sensors (102) are arranged in cooperation with the positioning protrusions (101).

6. A filtering device for biological sample detection according to claim 1, characterized in that: The filtering unit (3) comprises a first slide (31) slidably arranged on the machine base (1) along the length direction of the machine base (1), a first driving component (32) arranged on the machine base (1) for driving the first slide (31) to slide, a second slide (33) slidably arranged on the first slide (31) along the height direction of the machine base (1), a clamp (34) arranged on the second slide (33), and a second driving component (35) arranged on the first slide (31) for driving the clamp (34) to rise and fall, wherein the clamp (34) is used to clamp the reverse filtering tube (6).

7. The filtering device for biological sample detection according to claim 1, characterized in that: The liquid transfer unit (4) comprises a third slide (41) slidably arranged on the machine base (1) along the length direction of the machine base (1), a third driving assembly (42) arranged on the machine base (1) for driving the third slide (41) to slide, a fourth slide (43) slidably arranged on the third slide (41) along the height direction of the machine base (1), a fourth driving assembly (44) arranged on the third slide (41) for driving the fourth slide (43) to slide, a pipette head (45) arranged on the fourth slide (43), a liquid collection pump (46) arranged on the machine base (1), and a liquid infusion hose (47) for connecting the liquid collection pump (46) and the pipette head (45), the pipette head (45) is plugged into and matched with a liquid collection head in a clean head storage area (24), and a head removal assembly (48) for removing a liquid collection head connected to the pipette head (45) is also arranged on the fourth slide (43).

8. A filtering device for biological sample detection according to claim 7, characterized in that: The suction head removal assembly (48) comprises a first servo motor (481) mounted on the fourth slide (43), a first screw rod (482) rotating on the fourth slide (43), a slider (483) sliding along the height direction of the machine base (1), a push rod (484) fixed on the slider (483), an abutment plate (485) arranged at one end of the push rod (484) away from the slider (483), and a return spring (486) arranged between the abutment plate (485) and the fourth slide (43), wherein the abutment plate (485) is arranged to contact the fourth slide (43). A guide column (487) is provided on the fourth slide (43), and the guide column (487) is slidably inserted and matched with the fourth slide (43). The return spring (486) is sleeved on the guide column (487), and one end of the return spring (486) is fixedly connected to the abutment plate (485), and the other end of the return spring (486) is fixedly connected to the fourth slide (43). The bottom end of the pipette head (45) passes through the abutment plate (485) and is inserted and matched with the liquid collection head in the clean suction head storage area (24), and the abutment plate (485) is abutted and matched with the top end of the liquid collection head.

9. The filtering device for biological sample detection according to claim 7, characterized in that: The liquid pump (46) comprises a plunger pump column (461) fixed on the machine base (1), a piston (462) slidably arranged in the inner cavity of the plunger pump column (461), a connecting rod (463) fixed on the piston (462), a push plate (464) fixed on the end of the connecting rod (463) away from the piston (462), and a fifth driving component (465) arranged on the machine base (1) for driving the push plate (464) to slide. The liquid outlet of the plunger pump column (461) is connected to the infusion hose (47).

10. A filtration method for biological sample detection, implemented using a filtration device for biological sample detection according to any one of claims 1 to 9, characterized in that: The steps include: S1. Place a clean reverse filter tube (6) in the filter tube placement area (21), place the outer sleeve (7) in the sample area to be processed (22), and put the sample mixture that has been added with the sample extract and vortexed into the outer sleeve (7), place a clean centrifuge tube in the filtered sample area (23), and place a clean liquid collection pipette tip in the clean pipette tip storage area (24); S2, controlling the transmission of the first conveyor belt (26), the second conveyor belt (27) and the third conveyor belt (28) through the control unit (5), and under the cooperation of the positioning protrusion (101) and the positioning sensor (102), the first conveyor belt (26), the second conveyor belt (27) and the third conveyor belt (28) are stopped after being transmitted to the designated position; S3. Under the action of the second driving component (35), the clamping jaw (34) moves downward and clamps the connecting plate (8), and simultaneously takes out a plurality of reverse filter tubes (6). Under the action of the first driving component (32), the reverse filter tube (6) is moved to above the outer sleeve (7) in the sample area to be processed (22), and the reverse filter tube (6) is inserted into the outer sleeve (7), so that the sample mixture in the outer sleeve (7) is filtered and enters the reverse filter tube (6), and then the clamping jaw (34) is controlled to move out of the sample area to be processed (22); S4, through the cooperation of the third drive assembly (42) and the fourth drive assembly (44), the pipette head (45) is aligned with the liquid collection head in the clean tip storage area (24), and the liquid collection head is placed on the pipette head (45); S5, controlling the pipette head (45) with the liquid collection pipette tip to move to the top of the reverse filter tube (6) of the sample area (22) to be processed, controlling the liquid collection pipette tip to extend into the reverse filter tube (6), and sucking the filtrate through the liquid collection pump (46); S6, controlling the liquid taking pipette tip with the filtrate to move to the top of the centrifuge tube in the filtered sample area (23), thereby transferring the filtrate into the centrifuge tube; S7, controlling the pipette head (45) to move the used liquid pickup tip to the top of the clean tip storage area (24), and withdrawing the liquid pickup tip from the pipette head (45) through the tip withdrawal assembly (48), so that the used liquid pickup tip falls into the clean tip storage area (24) to complete the recovery; S8, control the first conveyor belt (26), the second conveyor belt (27) and the third conveyor belt (28) to continue to be transmitted to the next position, repeat the above steps to perform a new round of sample filtering, and the staff can take the filtered samples from the filtered sample area (23).

Images